Etiology Of Septicemia Research Articles

Molecular epidemiological studies on avian pathogenic Escherichia coli associated with septicemia in chickens in India

Bacterial septicemia causes huge economic losses in the poultry industry and there is no systematic research available in India on the connection of various pathogens associated with septicemia. The present molecular epidemiological study was conducted to investigate the association of different bacterial and immunosuppressive viral pathogens in septicemia suspected chickens. A total of 443 chicken carcasses with septicemic conditions from 71 different flocks were included in this study. Heart blood swabs were subjected to bacterial culture for Salmonella spp., Pasteurella multocida, Escherichia coli, and Gallibacterium anatis. Of these 51 flocks tested for E. coli, 49 (96.1%) flocks were found positive. Among flocks tested for Salmonella spp., 2 flocks were found positive. All tested flocks were found negative for G. anatis and P. multocida as well as air sac swabs tested negative for Mycoplasma spp. Bacterial cultural examination revealed that majority of septicemic chickens were found to be infected with E. coli and these E. coli isolates showed the highest resistance to vancomycin (60%), followed by erythromycin (50%) and cefotaxime (38%) and maximum sensitivity to cefotaxime and clavulanic acid combinations (81.5%), followed by chloramphenicol (69.6%) and ertapenem (67.2%). Among the 5 avian pathogenic E. coli (APEC) virulence genes were detected in 36 flocks and highest frequency of iss (100%), followed by ompT or iutA (97.2%), hly (61.1%) and iroN (47.2%) genes. On polymerase chain reaction (PCR) screening, 10.5, 4.5, 52.2, 19.4, 9.0, 4.5, 20.1 and 19.4% of the flocks were positive for G. anatis, Ornithobacterium rhinotracheale, APEC, Salmonella spp., Mycoplasma gallisepticum, Mycoplasma synoviae, chicken infectious anemia virus and Marek's disease virus, respectively. To our knowledge, the present study is first on the etiology of septicemia in chicken flocks in India. The present study infers that the majority of septicemic deaths in broiler chickens less than 8 weeks have been connected with APEC and majority of E. coli isolates are multidrug resistance, suggesting the need for surveillance and intervention to curb the inadvertent use of antibiotics. Although, incidence of G. anatis association with septicemia was reported, still requires a rigorous epidemiological study to determine the actual prevalence. However, more detailed studies encompassing vast geographical area with large sample size and long duration of the studies are necessary to provide a clear picture of the interaction of different pathogens causing septicemia in chicken.

Etiology of Septicemia in Children With Acute Leukemia

Bloodstream infection is an important cause of death among leukemia patients, and the etiologic agent surveillance is important for the prophylaxis and treatment. This study aims to identify the common bloodstream isolates in hospitalized leukemia patients with septicemia in our hospital, to choose the ideal combination of antimicrobial agents for infection prophylaxis and to clarify the appropriate time for antibiotic prophylaxis. To know this information, a retrospective analysis was conducted over a 9-year period from July 2001 to July 2010 by reviewing medical records of leukemia children admitted to our hospital. The overall frequencies of isolation were 45% in Gram-positive bacteria, 53.8% in Gram-negative bacteria, and 1.2% in fungi, respectively. Coagulase-negative Staphylococci were the most common organisms isolated, accounting for 32.7% of the total blood culture isolates, followed by Escherichia coli (15.7%) and Klebsiella pneumoniae (7.1%). The incidence of septicemia caused by extended-spectrum β-lactamase-producing E. coli and K. pneumoniae was high (69.2% and 58.8% of total isolates, respectively). The coverage rate of antimicrobial combinations of "vancomycin+cefoperazone-sulbactam" and "vancomycin+piperacillin-tazobactam" to blood culture isolates of leukemia patients in our hospital were 91.88% and 90.27%, respectively. More than 90% of septicemia occurred when the absolute neutrophil count was lower than 1.6×10⁹/L and 83.05% when absolute neutrophil count was lower than 1.0×10⁹/L. These results suggest that ongoing surveillance for antimicrobial susceptibility in leukemia children remains essential. Vancomycin+cefoperazone-sulbactam and vancomycin+piperacillin-tazobactam are the good choice for leukemia children to prevent bacterial infections in our hospital. In an effort to reduce total consumption of antibiotics and to elevate the therapeutic efficacy, antibiotics prophylaxis should be started with the appearance of neutropenia in leukemia children.

Septicaemia in a population-based HIV clinical cohort in rural Uganda, 1996-2007: incidence, aetiology, antimicrobial drug resistance and impact of antiretroviral therapy

To describe the incidence and aetiology of septicaemia, and antimicrobial drug resistance in HIV-infected and uninfected individuals, and the impact of antiretroviral therapy (ART) on septicaemia. Between 1996 and 2007, we followed up a rural population-based cohort of HIV-infected and uninfected participants. The aetiology and incidence of septicaemia, and antimicrobial drug resistances were determined. ART became available in 2004, and its impact on the incidence of septicaemia was examined. The overall septicaemia incidence (per 1000 pyrs) was 32.4 (95% CI 26.2-40.6) but was only 2.6 (95% CI 1.3-6.2) in HIV-negative patients and 67.1 (95% CI 53.4-85.4) in HIV-positive patients not on ART. Among those on ART, the overall incidence was 71.5 (95% CI 47.1-114.3), although it was 121.4 (95%CI 77.9-200.4) in the first year on ART and 37.4 (95%CI 18.9-85.2) in the subsequent period. Septicaemia incidence was significantly associated with lower CD4 counts. The commonest isolates were Streptococcus pneumoniae (SPN, n = 68) and Non-typhi salmonellae (NTS, n = 42). Most SPN isolates were susceptible to ceftriaxone and erythromycin, while resistance to cotrimoxazole and penicillin was common. All NTS isolates were susceptible to ciprofloxacin, but resistance to cotrimoxazole and chloramphenicol was common. Septicaemia incidence was higher in HIV-infected than in HIV-uninfected participants, and it remained high for some time among those who started ART. Starting ART earlier at higher CD4 counts is likely to lead to lower septicaemia incidence. Both SPN and NTS, the commonest isolates, were resistant to most commonly available antimicrobials. Blood culture laboratory surveillance systems to monitor antibiotic susceptibility and inform treatment guidelines are needed in Africa.

Septicemia in patients with hematological disorders and neutropenia: A retrospective study of causative agents and their resistance profile

In order to identify the cause of septicemia and the resistance patterns of bacteria in Swedish patients with hematological disorders, all positive blood cultures collected at a hematological ward during 1980-1986 were evaluated retrospectively. 198 episodes of septicemia in 129 patients were recorded. 54% were males and 46% women with a median age of 67 years (range 16-88). Patients with acute leukemia (46%), lymphoma (19%) and myeloma (19%) dominated. The absolute neutrophil count (ANC) was less than 0.5 x 10(9)/l in 76% of the bacteremic episodes. A total of 253 consecutive isolates were found with 53% Gram-negatives and 47% Gram-positives. The dominating pathogens were Escherichia coli (27%), klebsiella/enterobacter (15%), pseudomonas (7%), coagulase negative staphylococci (13%), alpha-streptococci (13%), Staphylococcus aureus (10%) and anaerobes (6%). Coagulase negative staphylococci showed a significant increase in isolation rate during the study period. The majority of E. coli were resistant to ampicillin. The susceptibility of klebsiella/enterobacter to ceftazidime and cefuroxime was reduced, while no imipenem resistant strains occurred. Among coagulase negative staphylococci 61% were resistant to isoxazolylpenicillin, none to vancomycin. No dramatic changes in the etiology of septicemia or the susceptibility pattern during the study period were noticed. Coagulase negative staphylococci, S. epidermidis in particular, constitute an increasing problem among granulocytopenic patients.

Changes in Incidence and Etiology of Septicemia in an Infectious Unit

AbstractIn a retrospective study from a department of internal medicine and infectious diseases, 81 patients with septicemia were recorded in a 5-year-period (1963–1967). This constitutes 1.25 cases per 100 admissions. 64 (79%) of the patients had positive blood cultures. The isolated bacteria were: Staphylococcus aureus (23%), gram-negative bacilli except salmonella (24%), gram-positive cocci other than S. aureus (40%), and Neisseria meningitidis and salmonella species (13%). 77 (95%) of the patients were not in hospital when they fell ill. The overall mortality was 25%. The mortality was highest among those infected with gram-negative bacilli (40%). There was a significant increase in the annual number of septicemias, mainly due to an increase in the frequency of septicemias caused by S. aureus. No significant increase in the septicemias caused by gram-negative bacilli was found. The frequency of antibiotic-resistant strains was unchanged during the survey. All isolated gram-negative strains except salm...

HEMOLYTIC STREPTOCOCCIC SEPTICEMIA

Septicemias caused by the streptococcus are among the most frequently encountered, as well as the most dreaded, of all general infections. In the greater percentage of cases, there is a fatal termination. Cooke 1 noted: All hemolytic streptococcic septicemias, with only occasional exceptions are characterized by being terminal, and are accompanied by an extremely severe toxemia. He tabulated the incidence of septicemia with positive blood culture that he observed at the St. Louis Children's Hospital, and noted that there were forty-eight cases of hemolytic streptococcic septicemia out of a total of 104 cases. This shows the great frequency of this particular strain of organism in the etiology of septicemia in infants and children. Again, he stated that in a group of sixty patients with streptococcic septicemia, fifty-one were due to hemolytic and nine to nonhemolytic strains of the organism. In only one instance in this series did recovery follow the