Age Estimation Research Articles

Dental age estimation methods applied to Indian children and adolescents: A systematic review and meta-analysis

Country-specific systematic reviews and meta-analyses have been proposed to compile the available literature and rank methods based on their performance for a target population. India is a country with a vast scientific literature on dental age estimation. This systematic review aimed to provide evidence to help the decision of experts regarding the method of choice for dental age estimation in India. The research protocol was registered in Open Science Framework. Literature Search was performed in Embase, LILACS, MedLine (via PubMed), SciELO, Scopus and Web of Science. Grey Literature was searched in Google Scholar and ProQuest. Observational cross-sectional studies that compared chronological and estimated ages using Demirjian (original [DEM] and Chaillet's modification [modified-DEM]) and Acharya (ACH) methods were included. JBI tool was used to assess the risk of bias. The search detected 9799 studies, out of which 56 were eligible (n=13,107 panoramic radiographs of Indian individuals). Low risk of bias was registered for 48 studies, while 8 presented a moderate risk of bias. The meta-analysis showed a standardized mean difference between chronological and estimated ages of -0.11 (95%CI: -0.29; 0.07), 0.74 (95%CI: 0.39; 1.09), and -0.01 (95%CI: -0.23; 0.22) years for DEM, modified-DEM and ACH, respectively. High heterogeneity (I2=88-93%) was observed across studies for all the methods, including subgroup analyses based on sex. This study ranked ACH, DEM and modified-DEM (from the best to the worse) performances in the Indian population.

Utilizing the tooth coronal pulp index for age estimation: A retrospective cone beam computed tomography study

Utilizing the tooth coronal pulp index for age estimation: A retrospective cone beam computed tomography study

Hidden Gems on a Ring: Infant Massive Clusters and Their Formation Timeline Unveiled by ALMA, HST, and JWST in NGC 3351

We use 0.1″ observations from the Atacama Large Millimeter Array (ALMA), Hubble Space Telescope (HST), and JWST to study young massive clusters (YMCs) in their embedded “infant” phase across the central starburst ring in NGC 3351. Our new ALMA data reveal 18 bright and compact (sub-)millimeter continuum sources, of which 8 have counterparts in JWST images and only 6 have counterparts in HST images. Based on the ALMA continuum and molecular line data, as well as ancillary measurements for the HST and JWST counterparts, we identify 14 sources as infant star clusters with high stellar and/or gas masses (∼105 M ⊙), small radii (≲ 5 pc), large escape velocities (6–10 km s−1), and short freefall times (0.5–1 Myr). Their multiwavelength properties motivate us to divide them into four categories, likely corresponding to four evolutionary stages from starless clumps to exposed H ii region–cluster complexes. Leveraging age estimates for HST-identified clusters in the same region, we infer an evolutionary timeline, ranging from ∼1–2 Myr before cluster formation as starless clumps, to ∼4–6 Myr after as exposed H ii region–cluster complexes. Finally, we show that the YMCs make up a substantial fraction of recent star formation across the ring, exhibit a nonuniform azimuthal distribution without a very coherent evolutionary trend along the ring, and are capable of driving large-scale gas outflows.

Estimation and probabilistic projection of age- and sex-specific mortality rates across Brazilian municipalities between 2010 and 2030.

Mortality rate estimation in small areas can be difficult due the low number of events/exposure (i.e. stochastic error). If the death records are not completed, it adds a systematic uncertainty on the mortality estimates. Previous studies in Brazil have combined demographic and statistical methods to partially overcome these issues. We estimated age- and sex-specific mortality rates for all 5,565 Brazilian municipalities in 2010 and forecasted probabilistic mortality rates and life expectancy between 2010 and 2030. We used a combination of the Tool for Projecting Age-Specific Rates Using Linear Splines (TOPALS), Bayesian Model, Spatial Smoothing Model and an ad-hoc procedure to estimate age- and sex-specific mortality rates for all Brazilian municipalities for 2010. Then we adapted the Lee-Carter model to forecast mortality rates by age and sex in all municipalities between 2010 and 2030. The adjusted sex- and age-specific mortality rates for all Brazilian municipalities in 2010 reveal a distinct regional pattern, showcasing a decrease in life expectancy in less socioeconomically developed municipalities when compared to estimates without adjustments. The forecasted mortality rates indicate varying regional improvements, leading to a convergence in life expectancy at birth among small areas in Brazil. Consequently, a reduction in the variability of age at death across Brazil's municipalities was observed, with a persistent sex differential. Mortality rates at a small-area level were successfully estimated and forecasted, with associated uncertainty estimates also generated for future life tables. Our approach could be applied across countries with data quality issues to improve public policy planning.

BATSCCD: a new change detection method for mapping building age in rapidly changing urban areas using Landsat time series data

ABSTRACT The building age is an important feature in the vulnerability assessment of disasters and building energy consumption modeling. It provides indispensable information for the study of built environment. However, obtaining high-precision maps of building ages is challenging, which impedes the development of relevant research and applications. In this study, a new building age mapping method based on Landsat time series classification and change detection (BATSCCD) was proposed, which integrates Landsat time series data with random forest and LandTrendr algorithm to reflect non-building to building changes, building reconstruction, and corresponding change characteristics. The proposed method achieved overall building age mapping accuracies of 80.45% within a 2-year tolerance and 87.66% within a 3-year tolerance in Guangzhou and accuracies of 78.08% and 87.52%, respectively, in Beijing. The improvement was notable, especially compared with the continuous change detection and classification (CCDC) method, with corresponding accuracy values of 36.22% and 46.31%, respectively, in Guangzhou and 49.7% and 60.19%, respectively, in Beijing. The results suggest that the BATSCCD method can be used to accurately estimate the building age and provide reliable mapping results. Thanks to the accessibility of required data and its high accuracy of building age estimation, the proposed method exhibits great application potential.

Multiple luminescence dating on heated materials at the nanzuo archaeological site, central Chinese Loess Plateau

Nanzuo is a key Neolithic archaeological site in the central Chinese Loess Plateau (CCLP). The site is important for understanding the origin and development of the Chinese civilization, yet, it lacks a robust chronological framework. The current work, for the first time, dates two different types of heated materials (pottery and burnt soil) from a profile near the main excavation of the Nanzuo archaeological site using various luminescence dating techniques. These techniques included single grain (SG) quartz OSL and K-feldspar post-infrared infrared-stimulated (pIRIR) dating for burnt soil samples and pottery, and pIRIR dating for pottery slices using the rock surface luminescence technique (RSL). The reliability of luminescence dating was investigated by comparing SG quartz, K-feldspar and pottery slice ages with radiocarbon (14C) ages obtained from the same cultural layer. The results show that the SG quartz OSL and K-feldspar pIRIR225 age estimates of the two coarse potteries are consistent with the 14C ages, however, the age estimates of the pottery slice (NZ22-CPS150) are significantly younger, which is primarily explained by underestimation in equivalent doses (De). Moreover, it is noted that while there is a considerable variation in the De of coarse pottery slices with depth, no significant correlation is observed between K- content and De. Heterogeneous K content was revealed by μ-XRF scanning on coarse pottery slices. Conversely, fine pottery displayed homogeneous K-content distribution and age estimates consistent with the 14C ages. Thus fine pottery slices are suggested to be more suitable for pIRIR dating relative to coarse pottery slices. The underestimation of De values in mixed minerals is observed as the primary reason for underestimated slice ages compared to SG K-feldspar, with grain size of K-feldspar and K content variation playing only a minor role. Therefore, by integrating the 14C ages with the luminescence ages of the pottery, it is inferred that human occupation of the Nanzuo site occurred around 5100 to 4500 years ago, which aligns with the 14C ages obtained from the main section of the Nanzuo site. The current work further concludes that the single-grain luminescence dating method is reliable for pottery. The study recommends to prioritize materials characterized by relatively uniform potassium (K) content for pIRIR dating using pottery slices.

Petrological, chemical, and chronological study of breccias in the Chang'e‐5 soil

AbstractWe carried out a petrological, mineralogical, and geochemical study of fragmental and regolith breccia clasts separated from two Chang'e‐5 (CE‐5) soil samples, CE5C0000YJYX03501GP and CE5C0400, which provide an opportunity to investigate the compositional change of regolith at the landing site through time. Fragmental breccia CE‐5‐B3 contains a diverse range of basaltic clasts and basaltic mineral fragments, and some rare Mg‐suite‐like minerals. Regolith breccias CE‐5‐B006, CE‐5‐B007, CE‐5‐B010‐08, CE‐5‐B010‐09, CE‐5‐B011‐07, and CE‐5‐B016‐03 contain mare basaltic fragments, mare vitrophyric clasts, rare Mg‐rich fragments possibly derived from the Mg‐suite rocks, and impact‐derived glass spherules. Pb‐isotope data obtained for baddeleyite grains found both inside some of the basaltic clasts identified in breccia fragments and in the breccia matrices yield Pb/Pb dates similar to the 2 Ga crystallization age of the CE‐5 basalt fragments, extracted directly from the soil sample. Seventy‐four Pb isotope analyses of Ca‐phosphate grains also indicate that the majority of these grains have Pb/Pb dates of 2 Ga, suggesting that they originate from the CE‐5 basalts. In addition, a Pb–Pb isochron drawn through analyses of four Ca‐phosphates in breccia CE5‐B006 yielded an intercept corresponding to a date of 3871 ± 46 Ma, which is the best possible estimate of the formation age of these four grains. Electron probe microanalysis shows that the breccias contain components similar to CE‐5 mare basalt fragments extracted directly from the soil sample, implying that the fragmental and regolith breccia fragments are mostly composed of material sourced from the underlying basalts. The general absence of impact melt breccia clasts, along with the general lack of Fe–Ni metal and absence of added meteoritic debris all suggest that the regolith at the CE‐5 landing site is immature and dominated by material mixed together by small local impact cratering events. Trace element analyses show that the glass beads in the regolith breccias have a Th abundance of 4.06–5.28 μg g−1. This is similar to the Th content of the regolith above the Em4 unit at the landing site as measured from orbit, as well as the estimated bulk Th content of CE‐5 basalts, suggesting that Th of the local regolith is predominantly sourced from the underlying mare basalts, without significant Th addition from Th‐rich exotic clasts sourced from evolved lunar lithologies.

Decreased but persistent epigenetic age acceleration is associated with changes in T-cell subsets after initiation of highly active antiretroviral therapy in persons living with HIV

IntroductionPersons living with HIV (PLWH) experience the early onset of age-related illnesses, even in the setting of successful human immunodeficiency virus (HIV) suppression with highly active antiretroviral therapy (HAART). HIV infection is associated with accelerated epigenetic aging as measured using DNA methylation (DNAm)-based estimates of biological age and of telomere length (TL).MethodsDNAm levels (Infinium MethylationEPIC BeadChip) from peripheral blood mononuclear cells from 200 PLWH and 199 HIV-seronegative (SN) participants matched on chronologic age, hepatitis C virus, and time intervals were used to calculate epigenetic age acceleration, expressed as age-adjusted acceleration residuals from 4 epigenetic clocks [Horvath’s pan-tissue age acceleration residual (AAR), extrinsic epigenetic age acceleration (EEAA), phenotypic epigenetic age acceleration (PEAA), and grim epigenetic age acceleration (GEAA)] plus age-adjusted DNAm-based TL (aaDNAmTL). Epigenetic age acceleration was compared for PLWH and SN participants at two visits: up to 1.5 years prior and 2–3 years after HAART (or equivalent visits). Flow cytometry was performed in PLWH and SN participants at both visits to evaluate T-cell subsets.ResultsEpigenetic age acceleration in PLWH decreased after the initiation of HAART but remained greater post-HAART than that in age-matched SN participants, with differences in medians of 6.6, 9.1, and 7.7 years for AAR, EEAA, and PEAA, respectively, and 0.39 units of aaDNAmTL shortening (all p < 0.001). Cumulative HIV viral load after HAART initiation was associated with some epigenetic acceleration (EEAA, PEAA, and aaDNAmTL), but even PLWH with undetectable HIV post-HAART showed persistent epigenetic age acceleration compared to SN participants (p < 0.001). AAR, EEAA, and aaDNAmTL showed significant associations with total, naïve, and senescent CD8 T-cell counts; the total CD4 T-cell counts were associated with AAR, EEAA, and PEAA (p = 0.04 to <0.001). In an epigenome-wide analysis using weighted gene co-methylation network analyses, 11 modules demonstrated significant DNAm differences pre- to post-HAART initiation. Of these, nine were previously identified as significantly different from pre- to post-HIV infection but in the opposite direction.DiscussionIn this large longitudinal study, we demonstrated that, although the magnitude of the difference decreases with HAART is associated with the cumulative viral load, PLWH are persistently epigenetically older than age-matched SN participants even after the successful initiation of HAART, and these changes are associated with changes in T-cell subsets.

FACIAL IMAGE CAPTIONING USING DNN

Facial analysis, encompassing emotion, age, and gender detection, shows potential in various applications such as human-computer interaction, business, security, and health. This study delves into the development and evaluation of a deep neural network (DNN) model for facial emotion, age, and gender detection. Utilizing a convolutional neural network (CNN) architecture trained on diverse datasets for each task, our model proves effective in predicting facial features. The accuracy of needs assessment is X%, the marginal error (MAE) of age estimation is Y years, and the accuracy of gender classification is Z%.

Exploiting the Greenland volcanic ash repository to date caldera-forming eruptions and widespread isochrons during the Holocene

Polar ice-cores have long been recognised as unrivalled repositories of past volcanic events. Although tephra products from local eruptions tend to dominate these records, improvements in micro-sampling and analytical techniques are uncovering a growing number of cryptotephras erupted from exceptionally distant volcanoes. We present a series of nine Middle Holocene cryptotephra deposits detected within the NGRIP ice-core that originate from five different volcanic regions across the Northern Hemisphere (Alaska, Cascades, Iceland, Japan, Kamchatka). Unique compositional signatures are employed to identify ash from three large caldera-forming events in Kamchatka (KS2 from Ksudach), the Cascades (Mazama) and North East Japan (Mashu), along with ash from the Hekla 4 eruption in Iceland. High-precision ice-core ages (adopting a 1950 CE datum for the GICC05 timescale assigned to the Greenland ice cores) are derived for each eruption: Hekla 4 (4325 ± 8 a b1.95k), KS2 (7089 ± 26 a b1.95k), Mashu (i-f) (7473 ± 33 a b1.95k) and Mazama (7562 ± 35 a b1.95k), all of which can be employed as chronological fix-points in other proxy records where these deposits are also preserved. Four further cryptotephra deposits and one macro-deposit are also identified and traced to sources in Iceland and Alaska. The cryptotephra originating from Alaska is correlated to a deposit identified in lake records from the Kenai Peninsula, thought to originate from Redoubt Volcano. The remaining four deposits are typical of the products of Katla, Grímsvötn and Veiðivötn in Iceland. This ensemble of Middle Holocene tephra deposits highlights the pivotal position of the Greenland ice-sheet and its ice-cores to capture deposition from the convergence of several far-travelled ash clouds. Precise age estimates derived from the annually resolved ice-core record greatly enhances the value of these tephra isochrons.

Kinematic Age of the β-Pictoris Moving Group

Accurate age estimation of nearby young moving groups (NYMGs) is important as they serve as crucial testbeds in various fields of astrophysics, including the formation and evolution of stars and planets, as well as loose stellar associations. The β-Pictoris moving group (BPMG), being one of the closest and youngest NYMGs, has been extensively investigated, and its estimated ages have a wide range from ∼10–25 Myr, depending on the age estimation methods and data used. Unlike other age dating methods, kinematic traceback analysis offers a model-independent age assessment hence the merit in comparing many seemingly discordant age estimates. In this study, we determine the kinematic ages of the BPMG using three methods: probabilistic volume calculation, mean pairwise distance calculation, and covariance matrix analysis. These methods yield consistent results, with estimated ages in the range of 14–20 Myr. Implementing corrections to radial velocities due to gravitational redshift and convectional blueshift increases the ages by ∼2–4 Myr. Conversely, considering data uncertainties decreases the estimated ages by 1–2 Myr. Taken together, our analysis determined the kinematic age of the BPMG to be 16.3−2.1+3.4 Myr. This age is significantly younger than the commonly accepted age of the BPMG (∼24 Myr) determined primarily from the lithium depletion boundary analysis. This younger kinematic age may point to the discrepancy between the luminosity evolution and lithium depletion models or the presence of unaccounted systematic error in the method. This result underscores the necessity for systematic reevaluations of age-dating methods for nearby, young moving groups.

The role of cortical structural variance in deep learning-based prediction of fetal brain age

BackgroundDeep-learning-based brain age estimation using magnetic resonance imaging data has been proposed to identify abnormalities in brain development and the risk of adverse developmental outcomes in the fetal brain. Although saliency and attention activation maps have been used to understand the contribution of different brain regions in determining brain age, there has been no attempt to explain the influence of shape-related cortical structural features on the variance of predicted fetal brain age.MethodsWe examined the association between the predicted brain age difference (PAD: predicted brain age–chronological age) from our convolution neural networks-based model and global and regional cortical structural measures, such as cortical volume, surface area, curvature, gyrification index, and folding depth, using regression analysis.ResultsOur results showed that global brain volume and surface area were positively correlated with PAD. Additionally, higher cortical surface curvature and folding depth led to a significant increase in PAD in specific regions, including the perisylvian areas, where dramatic agerelated changes in folding structures were observed in the late second trimester. Furthermore, PAD decreased with disorganized sulcal area patterns, suggesting that the interrelated arrangement and areal patterning of the sulcal folds also significantly affected the prediction of fetal brain age.ConclusionThese results allow us to better understand the variance in deep learning-based fetal brain age and provide insight into the mechanism of the fetal brain age prediction model.

Performance of Artificial Intelligence Models Designed for Automated Estimation of Age Using Dento-Maxillofacial Radiographs—A Systematic Review

Performance of Artificial Intelligence Models Designed for Automated Estimation of Age Using Dento-Maxillofacial Radiographs—A Systematic Review

Alligator gar as a centenarian species: extending lifespan estimates using bomb radiocarbon and laser ablation–accelerator mass spectrometry

Context The potential lifespan of alligator gar (Atractosteus spatula) is currently unknown. A previous study applied bomb radiocarbon (14C) dating to establish ages >60 years, but maximum age was limited by the rise of bomb-produced 14C in the 1950s. Aims An exceptionally large specimen (2.6 m, 148 kg) was captured in Mississippi and otolith growth zones revealed an age estimate of 95 years. This specimen provided an opportunity to apply new technology to validate the lifespan of alligator gar. Methods Developments in accelerator mass spectrometry (AMS) led to technology that measures 14C continuously from carbonates (laser ablation-AMS), as opposed to single sample 14C analysis. Key results Use of laser ablation AMS on the 2.6-m alligator gar otolith, and two smaller fish aged >60 years, supported ages that were older than original estimates by 5–20 years. Conclusions Our study indicates that alligator gar age can be underestimated for the largest fish, maximum recorded length is 2.6 m based on a historical photograph, and lifespan is at least 75–95 years with support for an age of 100 years for the 2.6-m fish. Implications An increase in lifespan of this magnitude would affect our understanding of population dynamics and recovery efforts.

Apparent diffusion coefficient and tissue stiffness are associated with different tumor microenvironment features of hepatocellular carcinoma.

To investigate associations between tissue diffusion, stiffness, and different tumor microenvironment features in resected hepatocellular carcinoma (HCC). Seventy-two patients were prospectively included for preoperative magnetic resonance (MR) diffusion-weighted imaging and MR elastography examination. The mean apparent diffusion coefficient (ADC) and stiffness value were measured on the central three slices of the tumor and peri-tumor area. Cell density, tumor-stroma ratio (TSR), lymphocyte-rich HCC (LR-HCC), and CD8 + T cell infiltration were estimated in resected tumors. The interobserver agreement of MRI measurements and subjective pathological evaluation was assessed. Variables influencing ADC and stiffness were screened with univariate analyses, and then identified with multivariable linear regression. The potential relationship between explored imaging biomarkers and histopathological features was assessed with linear regression after adjustment for other influencing factors. Seventy-two patients (male/female: 59/13, mean age: 56 ± 10.2 years) were included for analysis. Inter-reader agreement was good or excellent regarding MRI measurements and histopathological evaluation. No correlation between tumor ADC and tumor stiffness was found. Multivariable linear regression confirmed that cell density was the only factor associated with tumor ADC (Estimate = -0.03, p = 0.006), and tumor-stroma ratio was the only factor associated with tumor stiffness (Estimate = -0.18, p = 0.03). After adjustment for fibrosis stage (Estimate = 0.43, p < 0.001) and age (Estimate = 0.04, p < 0.001) in the multivariate linear regression, intra-tumoral CD8 + T cell infiltration remained a significant factor associated with peri-tumor stiffness (Estimate = 0.63, p = 0.02). Tumor ADC surpasses tumor stiffness as a biomarker of cellularity. Tumor stiffness is associated with tumor-stroma ratio and peri-tumor stiffness might be an imaging biomarker of intra-tumoral immune microenvironment. Tissue stiffness could potentially serve as an imaging biomarker of the intra-tumoral immune microenvironment of hepatocellular carcinoma and aid in patient selection for immunotherapy. Apparent diffusion coefficient reflects cellularity of hepatocellular carcinoma. Tumor stiffness reflects tumor-stroma ratio of hepatocellular carcinoma and is associated with tumor-infiltrating lymphocytes. Tumor and peri-tumor stiffness might serve as imaging biomarkers of intra-tumoral immune microenvironment.

Using seasonal oscillations in fin ray microchemistry to chemically age Lake Sturgeon

AbstractObjectiveNonlethally sampled pectoral fin rays are commonly used as aging structures for Lake Sturgeon Acipenser fulvescens, but they tend to underestimate the true age in older, slow‐growing individuals (age &gt; 14 years). Current aging practices involve counting bands along the structure, which are construed as annuli. Oscillations of certain trace elements corresponding with annuli have been seen across various fish species, with patterns continuing to the marginal edge of hard structures. This study explored the aging of fin rays by using microchemistry patterns in Lake Sturgeon of known age (n = 94; ages 5–21) to determine the potential use of this method as an alternative or supplementary aging technique.MethodsElements were chosen for age determination analysis by examining the relationship between profile minima/maxima and visually interpreted annuli. Fish were assigned ages using three different methods: (1) traditional interpretation (counting annuli along the structure), (2) chemical interpretation (examination of seasonal variations in elemental profiles with visually identified annuli), and (3) a statistical model (multivariate multiple changepoint analysis with finite differencing using raw elemental profiles).ResultMean absolute differences between age estimates and known age were significantly higher for the traditional interpretation method than for the chemical interpretation method. The mean coefficient of variation in estimated age was 11.14% for the traditional interpretation method and 4.04% for the chemical interpretation method. The changepoint model was able to correctly classify age for 100% of the samples within ±1 year in one population (ages 5–8) but could not classify samples from the second population (ages 12–21).ConclusionOur results suggest that chemical aging techniques could provide more reliable age estimates for juvenile and subadult Lake Sturgeon when fin rays are the only aging option. Further work is required to determine the applicability of the model for assigning ages to older fish and for use with different populations and structures.

Amino acid dating of Pleistocene mammalian enamel from the River Thames terrace sequence: a multi-taxon approach

Amino acid geochronology can provide effective relative dating frameworks for the Pleistocene and has enabled correlation of terrestrial deposits to the global climatic fluctuations described by the marine oxygen isotope record. Using methods developed for the analysis of intra-crystalline amino acids in tooth enamel, we aimed to construct an enamel-based amino acid geochronology for the terrace deposits in the valley of the River Thames in southern Britain using different mammalian taxonomic groups: elephant, horse and bison. To achieve this, chiral amino acid analysis was applied to 58 elephantid, 21 horse and 15 bison teeth from 10 horizons in the Upper Thames Valley, three in the Lower Thames Valley and one from a Thames tributary in the Lea Valley. We evaluate differences in the rates of amino acid breakdown between the taxa and establish which species are similar enough to enable comparison for relative dating purposes. The relative dating of the river terrace deposits is in good agreement with the terrace stratigraphy, biostratigraphy, and other independent estimates of age for all three taxonomic groups. These frameworks demonstrate the potential of enamel-based amino acid geochronologies for relative dating of Middle–Late Pleistocene deposits in the UK, and establish an aminostratigraphic framework from which the dating of other tooth material can be refined. Enamel offers an opportunity to evaluate the age of sites where shell material is absent or poorly preserved. It can also, crucially, provide direct relative dating of mammalian fossils, which are often the focus of study in terms of their evolution, distributional changes or extinction. Direct dating negates the risk that the mammal fossils themselves might be reworked, or of different ages to shell, sediments or other dated material in the same deposits; it also enables archived samples with insecure provenance (e.g. from early 17th-19th century collections) to be directly dated.

Data-Driven Patient clusters of Type 2 Diabetes May Help to Identify Patients at Risk and Predict Metabolic Surgery Outcomes on Diabetes Remission and NAFLD Pregression

Abstract Background Precision medicine and customized medicine have gained enormous attention in recent years, especially in the treatment of type 2 diabetes (T2D). Different subgroups of diabetes have been identified by research employing data-driven cluster analysis, each with a unique diabetes progression and complication risk (Ahlqvist et al., Lancet Diabetes Endocrinol. 2018). Aims We aimed to apply the proposed cluster analysis to a patent population post metabolic surgery and investigate the association with T2D remission and presence of NAFLD. Methods We retrospectively linked newly defined clusters to metabolic surgery outcomes in 53 T2D patients. Utilizing k-means and hierarchical clustering, three clusters emerged based on glutamate decarboxylase antibodies, age, BMI, HbA1c, and homoeostatic model assessment estimates of β-cell function (HOMA2-B%) and insulin resistance (HOMA2-IR). Intraoperative liver biopsies assessed nonalcoholic fatty liver disease (NAFLD) presence differentiating between simple steatosis (NAFL) and steatohepatitis (NASH). Clinical and biochemical data were collected over two years, focusing on T2D remission and NAFLD improvement. Results Cluster 1, characterized by the lowest BMI, highest NASH rate, impaired beta-cell function, and increased insulin resistance, displayed a favorable response to surgery, indicating robust regeneration of beta-cells. Despite increased insulin production, T2D remission was surprisingly low at 44.0% after one year, in contrast to 75.00% in Cluster 2 and 100.00% in Cluster 3. Metabolic surgery notably reduced insulin resistance and promoted NASH remission, evidenced by a significant reduction in a non-invasive NASH detection score and liver enzyme levels across all three clusters. Conclusion Our findings suggest that patients in Cluster 1 already show a lack of beta-cell compensation being associated with a higher prevalence of NAFLD and poorer diabetic control and therefore especially might benefit from an earlier intervention. Data-driven classification might help to customize treatment plans and identify patients at higher risk of problems at diagnosis.

Forensic age estimation at the University Center of Legal Medicine Lausanne-Geneva: a retrospective study over 12years.

With the undeniable increase in asylum requests from unaccompanied alleged minors, age estimation of living individuals has become an essential part of the routine work in European forensic centers. This study aims to review the forensic age estimations performed in our center since 2010, to evaluate the state-of-the-art of this practice in Switzerland with the evolution of the methodology according to upcoming recommendations. Our institute's expert reports performed between 2010 and 2022 were retrospectively analyzed. We gathered the following parameters: demographic data, morphological characteristics, alleged age compared with the assessed minimum age, sexual maturation, dental and bone age. When available, we collected personal and family history, medical history, records of torture-related/self-inflicted injuries, and information about eating habits that might affect skeletal development. Data collection amounted to 656 cases. Forensic age estimations ordered by the Swiss Secretariat for Migration (SEM) represented 76.4% of cases, with 23.6% of them ordered by the Court/Public Prosecutor. Most alleged minors were male (94.5%) and came from Afghanistan (53.4%). Adjunction of CT scans of the sternoclavicular joints was necessary in 86.4% of cases. Only 25.2% of our reports concluded on most probable minority, with 55.6% of definite majors; in 19.2% of our cases, minority could not be excluded. This study aspires to further broaden our expertise regarding forensic age estimations. Given the increasing migratory flows, we can expect a notable increase in the frequency of these requests. Consequently, this study aims to promote a multidisciplinary approach and the international standardization of the methodology of these estimations.

Comparative evaluation of two different dental age estimation methods using tooth pulp: A CBCT study.

To estimate the age of an individual based on 3D radiographic evaluation of pulp width of maxillary central incisor. This study included 185 CBCT images of individuals within age range of 14-64 years. Images were evaluated for maxillary central incisors and pulp width measurements were taken from cementoenamel junction and middle third of root. Obtained data was subjected to correlation and regression analysis from which the age of an individual was predicted. Results of the present study were compared with another study by the same authors. A negative linear relationship was obtained between age and pulp width. The standard error of estimate (SEE) in sagittal section was 11.36 years and that in coronal section was 11.23 years. The coefficient of determination for sagittal section was 0.107 and for coronal section was 0.127. An obtained regression formula was highly significant. Division of samples into various age groups reduced SEE drastically. It can be concluded that pulp width of maxillary central incisor is a reliable indicator of age estimation.