Cumin Seeds Essential Oil Research Articles

Vaginal suppositories of cumin seeds essential oil for treatment of vaginal candidiasis: Formulation, in vitro, in vivo, and clinical evaluation

Fungal infections by resistant Candida species continue to be a significant health problem. Novel antifungal agents such as essential oils of cumin seeds (EOCS) are tested against vulvovaginal candidiasis (VVC). The aim of this study was to develop coated polyethylene glycol (PEG) vaginal suppositories containing EOCS for treatment of VVC. PEG suppositories containing EOCS were prepared ppearance, weight variation, drug content, hardness, dissolution time, release, stability and anticandida activity were evaluated. Biocompatibility of selected formulation was tested in female rabbits, followed by clinical evaluation. Coated suppositories showed complete release of the oil after 30 min, in vitro anti-candida activity, enhanced stability and sufficient safety on the vaginal tissues of rabbits. Clinical results showed significant lower rates of vaginal itching, discharge and dyspareunia combined with negative cultures in 70% of patients, revealing efficacy of EOCS-containing vaginal suppositories for treatment of VVC.

Formation and characterization of calcium alginate hydrogel beads filled with cumin seeds essential oil

Preparation and characterization of novel encapsulation system based on calcium alginate hydrogels filled with cumin essential oil has been investigated. Firstly, the effect of sodium alginate concentration, CaCl2 level, hardening time, encapsulation and emulsion fabrication methods was studied on loading capacity of the hydrogels using a Resolution-V fractional factorial design (2 V5−1 FFD), followed by response surface methodology (RSM). At the optimum point, the in-vitro release of phenolic compounds in simulated gastric and intestinal mediums were 96.02 ± 0.96% and 10.65 ± 1.23% after 180 min, respectively. The Scanning electron microscopy (SEM) images demonstrated a relatively smooth surface with small pore size. Based on SEM images and Fourier-transform infrared spectrums, the cumin essential oil was encapsulated successfully in calcium alginate beads. Thus, calcium alginate hydrogel could be introduced as a promising carrier for encapsulating biochemical active compounds with favorable features.

Yield, Quality and Composition of Cumin Essential Oil as Affected by Storage Period

Essential oils from newly produced (2017) and previous season crop (2016) of cumin (Cuminum cyminum L.) seeds collected from local farmers in Dongla area, north Sudan were extracted and analyzed to determine the effect of storage period on volatile oil yield and quality. The essential oil was extracted using hydro distillation method. Identification of the volatile compounds was performed using gas chromatography-mass spectrometry (GC/MS). Results revealed that oil obtained from cumin (Cuminum cyminum L.) was pale yellow. With respect to oil characteristics, the specific gravity, refractive index, acid value, ester value and other quality characters were not significantly different between the stored and newly harvested seeds. Thirty-eight compounds were separated and identified in cumin seeds essential oil in both seasons with some minor differences in some components between 2016 and 2017 samples. Major effective components of cumin seeds volatile oil were cumin alcohol (4-isopropylbenzyl alcohol), cumin aldehydes as 4-Isopropylbenzaldehyd and 7,7-dimethylbicyclo (4.1.0) hept-3-ene-4-carbaldehyde known as carenal, terpinenes as γ-terpinene and α-terpinene (1,4-Cyclohexadiene, 1-methyl-4-(1-methylethyl) and 1,3-cyclohexadine-1-methanol, 4(1-methethyle)-), β-pinene, o-cymene and p-menthadienol in both stored and newly harvested samples. Differences between the samples season wise were very minor with respect to type of volatile components but percentage oil yield was largely decreased by storage period.

Effect of Drying Methods on Chemical Composition and Biological Activity of Essential Oil from Cumin (Cuminum cyminum L.)

The effect of air drying (AD), hot-air oven drying (OD), and infrared drying (ID) on chemical composition and bioactivity of cumin seeds essential oil are investigated in this study. The results showed that different drying methods resulted in the differences in yield, chemical composition, antioxidant and antibacterial activity as well as DNA damage protective effect of essential oil from cumin seeds. Drying resulted in losses or increases of some volatile components or production of new composition. However, no difference in main compositions was found among samples. In general, OD40 (at 40°C) possessed the higher antioxidant, antibacterial activities and DNA damage protective effect, followed by AD, ID40 (at 40°C), and OD60 (at 60°C), the lowest for ID60 (at 60°C) sample. In addition, higher drying temperature was easier to result in the decrease of yield and biological activity of essential oil from cumin seeds.

The analysis of cumin seeds essential oil and total polyphenols from postdestillation waste material

The essential oil content in cumin samples from Serbian market ranged between 2.0 and 4.0%, with 22 identified compounds, among which the most abundant were cumin aldehyde, β-pinene, γ-terpinene, γ-terpinene-7-al and p-cymene. Postdistillation cumin seeds waste material that remained after the essential oil extraction contains total polyphenols of between 30.1 and 47.5 mg GAE/g dry extract, as estimated by the Folin-Ciocalteu method. Hydroxybenzoic and hydroxycinnamic acids, as well as glycosides of flavonones and flavonoles, are the dominant polyphenols. However, according to DPPH method, the antioxidative potential of cumin postdistillation seeds waste was poor and it ranged between 0.02 and 0.04 mM TE/g. Further research will be focused on agro-food implementation of postdistillation waste material of cumin and other plants which are used for the essential oil production.