Eucalyptus Globulus Essential Oil Research Articles

Eucalyptus globulus Essential Oil modulates Genotoxicity and Histological Alterations Induced by Mitomycin C in Mice

Eucalyptus globulus Essential Oil modulates Genotoxicity and Histological Alterations Induced by Mitomycin C in Mice

In Vitro Effect of Eucalyptus Essential Oils and Antiseptics (Chlorhexidine Gluconate and Povidone-Iodine) against Bacterial Isolates from Equine Wounds.

Considering the increasing antibiotics resistance, there has been a propensity to replace them with antiseptics when it comes to wound management and treatment. Nevertheless, in recent years, there have been reports regarding resistance to antiseptics by some bacterial strains. There is also concern about the environmental impact of these substances. The aim of this study was to compare the antimicrobial efficacy of antiseptics and eucalyptus essential oils on bacterial strains from horse's wounds. We used twelve Escherichia coli, eight Staphylococcus aureus, two Staphylococcus pseudintermedius, one Staphylococcus vitulinus and one Staphylococcus saprophyticus strains from equine wounds. The effect of Eucalyptus radiata essential oil, Eucalyptus globulus essential oil, povidone-iodine and chlorhexidine gluconate against the isolated strains was evaluated applying the Kirby-Baüer method. Regarding the Escherichia coli strains, E. radiata and the mixture of E. radiata and E. globulus had a better inhibitory effect than antiseptics. E. globulus had a better effect against most Staphylococcus spp. compared to E. radiata. For both Gram-negative and Gram-positive strains tested, chlorhexidine gluconate had a better inhibitory effect than povidone-iodine. The antibacterial efficacy of essential oils highlights their potential to substitute or complement the use of antiseptics and so reduce resistance to antiseptics.

Bioactive, antioxidant and antimicrobial properties of chemically fingerprinted essential oils extracted from Eucalyptus globulus: in-vitro and in-silico investigations.

Innovative approaches are urgently required to treat divestating bacterial diseases in the face of rising bacterial resistance rates. The current investigative work focused on hydro-distilling Tasmanian blue gum (Eucalyptus globulus) to isolate the essential oil, which was then tested for bioactivity, antioxidant capacity, and antibacterial activity using in-vitro and in silico assays. The antioxidant activity was avualated against DPPH and FRAP. With results of 69.63 RSA (%) (µL/L AAE) at a concentration of 5mL/L and 51.56 (µL/L AAE) at concentration of 90ppm in the 2,20-diphenyl-1-picryl hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays, respectively, the extracted oil indicated considerable antioxidant activity. The extracted oil demonstrated powerful antibacterial activity in in-vitro tests against both Gram-positive and Gram-negative bacterial strains, including Bordetella bronchiseptica (21mm), Staphylococcus epidermidis (19mm), and Staphylococcus aureus (19mm), with significant minimal inhibitory (MIC) and minimum bactericidal (MIB) concentrations. Additionally, GC-MS analysis of the oil from E. globulus identified several low-molecular-weight compounds, including Eucalyptol, γ-Terpinene, Shisool acetate, 1,3-trans,5-cis-Octatriene, 2,6-Dimethyl-1,3,5,7-octatetraene, E,E, Cyclohexene, 1-methyl-4-(1-methylethylidene), Benzene, 1-methyl-4-(1-methylethenyl), Butanoic acid, 3-methyl-, 3-methylbutyl ester, and 1,3,8-p-Menthatriene. Several other compounds were also identified, including Fenchol, 2-Methyl-trans-3a,4,7,7a-tetrahydroindane, (E,E,E)-2,4,6-Octatriene, 1,2,3,6-Tetrahydrobenzylalcohol, acetate, Alloaromadendrene, Phenol, 2-ethyl-4,5-dimethyl, Phenol, 2-methyl-5-(1-methylethyl)-, p-Cymen-7-ol, 1,5,5-Trimethyl-6-methylene-cyclohexene, 1,3-Cyclohexadiene, 1-methyl-4-(1-methylethyl)-, 2,6-Octadien-1-ol, 3,7-dimethyl-, acetate, (Z), and more. The bioactive potential of Eucalyptus globulus essential oil against 1AJ6 and 1R4U was highlighted by molecular docking analyses, suggesting its utility as a natural source of antioxidant and antibacterial compounds with the potential to replace chemical disinfectants in a variety of applications.

Electrospun polyvinyl alcohol/chitosan nanofibers incorporated with 1,8-cineole/cyclodextrin inclusion complexes: Characterization, release kinetics and application in strawberry preservation.

Development of food packaging systems containing essential oils (EOs) has gained increased attention recently. However, the instability of EOs restricts their application. Therefore, effective encapsulation of EOs is demanded for their protection and controlled release. In this work, 1,8-cineole, the major component in Eucalyptus globulus essential oil, was encapsulated into hydroxypropyl-β-cyclodextrin to form an inclusion complex, which was then incorporated into polyvinyl alcohol and chitosan composite polymer to fabricate nanofibrous film via electrospinning. The film with 40% (w/w) of inclusion complexes showed enhanced barrier and mechanical properties, and the release of 1,8-cineole from the film was sustained and dominated by the non-Fick diffusion. Moreover, this film could extend the shelf life of strawberries to 6days at 25 ℃. This work suggested dual encapsulation of EOs by cyclodextrin and electrospun nanofibers is an ideal strategy to improve the availability of EOs, and the produced film is promising for food preservation.

Eucalyptus globulus essential oil thin film polymerized by cold plasma on Ti6Al4V: Sterilization effect, antibacterial activity, adhesion, and viability of mesenchymal stem cells

AbstractThis work aims to evaluate the characterization, antibacterial activity, and mesenchymal stem cell (MSC) viability in films obtained by cold plasma polymerization, from the essential oil of Eucalyptus globulus on Ti6Al4V alloy. The film presented low roughness values and a contact angle of 85°. The process incorporated a coating with oxygenated functional groups (OH and C═O). The effect of ultraviolet radiation, ethylene oxide, autoclave, and air plasma sterilization on polymer degradation was evaluated. Biofilm formation by Staphylococcus aureus was reduced on the film surface with no toxicity to MSCs. These effects can be attributed to functional groups, combined with the surface roughness and contact angle. These results suggest a potential for the application of this coating in biomaterials.

Insecticidal efficacy and physiological effects of Eucalyptus globulus essential oil and its constituent, 1,8-Cineole against Tribolium confusum (Jacquelin du Val, 1868) (Coleoptera, Tenebrionidae)

Insecticidal efficacy and physiological effects of Eucalyptus globulus essential oil and its constituent, 1,8-Cineole against Tribolium confusum (Jacquelin du Val, 1868) (Coleoptera, Tenebrionidae)

Anti-inflammatory mechanisms of eucalyptol rich Eucalyptus globulus essential oil alone and in combination with flurbiprofen.

Inflammation is the core contributor in the pathogenesis of various acute and chronic illness including appendicitis, bronchitis, arthritis, cancer and neurological diseases. NSAIDs, commonly used medications for inflammatory diseases, on prolonged use cause GI bleeding, ulcers and many more issues. Plant-based therapeutic agents including essential oils in combination with low-dose synthetic drugs have been shown to produce synergistic effects and reduce complications of synthetic drugs. This study was designed to evaluate the anti-inflammatory, analgesic and anti-pyretic properties of Eucalyptus globulus essential oil alone and in combination with flurbiprofen. GC-MS analysis was performed to screen chemical composition of oil. In vitro anti-inflammatory assay (membrane stabilization assay) and in vivo inflammatory acute(carrageenan and histamine-induced paw oedema)and chronic (cotton pellet-induced granuloma and Complete Freund's adjuvant-induced arthritis)modelswere performed to check anti-inflammatoryproperties. Acetic acid-induced algesia and yeast-induced pyrexia models were performed to check analgesic and anti-pyretic properties. qRT-PCR was performed to study the effect of treatments on the expression of inflammatory biomarkers. GC-MS analysis of E. globulus essential oil showed the presence of eucalyptol along with other active biomolecules. 500 + 10mg/kg of oil-drug combination showed significantly (p < 0.05) better in vitro membrane stabilization effects as compared with groups treated with 500mg/kg of E. globulus oil and10mg/kg of Flurbiprofenalone. 500 + 10mg/kg of oil-drug combination showed significantly (p < 0.05) better anti-inflammatory, analgesic and antipyretic effects as compared to 500mg/kg of E. globulus oil alone in all in vivo models. When comparison wasdone between 500 + 10mg/kg of oil-drug combination-treated and 10mg/kg Flurbiprofen-treated group, the former group showed significantly (p < 0.05) better anti-inflammatory and anti-pyretic effects, but there were non-significant differences in the analgesic model. Animal grouptreated with10mg/kg of Flurbiprofen showed significantly (p < 0.05) better anti-inflammatory and analgesic effects than group treated with500mg/kg of oil alone while, there were non-significant differences in anti-pyretic effects. qRT-PCR analysis showed significant (p < 0.05)down-regulation in the expressionof IL-4 and TNF-α in serum samples of animals treated with 500 + 10mg/kg of oil-drug combination as compared to thediseased control (arthritic)group. Overall, the current research demonstrates that Eucalyptus globulus essential oil in combination with flurbiprofen showed better anti-inflammatory, analgesic and anti-pyretic effects than oil and flurbiprofen alone which is attributed to the down-regulation of pro-inflammatory biomarkers (IL-4 and TNF-α). Further studies are required to formulate a stable dosage form and to check the anti-inflammatory efficacy in different inflammatory disorders.

Synergistic Inhibition of Methicillin-Resistant Staphylococcus aureus (MRSA) by Melaleuca alternifolia Chell (Tea Tree) and Eucalyptus globulus Labill. Essential Oils in Association with Oxacillin.

The presence of antibiotic-resistant bacteria has become a major therapeutic priority. This trend indicates the need for alternative agents to antibiotics, such as natural compounds of plant origin. By assessing membrane permeability, we investigated the antimicrobial activity of Melaleuca alternifolia and Eucalyptus globulus essential oils (EOs) against three strains of methicillin-resistant Staphylococcus aureus (MRSA). Using the checkerboard method, the efficacy of single EOs, in association with each other or in combination with oxacillin, was quantified by calculating the fractional inhibitory concentrations (FIC Index). All EOs showed a reduction in bacterial load, an alteration of membrane permeability which leads to an increase in its function, resulting in the release of nucleic acids and proteins. The treatment with EO-oxacillin combinations and associated EO-EO resulted in a synergistic effect in most of the tests performed. EO-EO association showed a high activity in the alteration of the membrane, increasing the permeability to about 80% in all the MRSA strains treated. In conclusion, the combination of EOs and antibiotics represents a valid therapeutic support against MRSA bacteria, allowing for a decrease in the antibiotic concentration needed for therapeutic use.

Climatological effects on Chemical Composition, Biological Activity of Essential oil from Eucalyptus globulus Leaves and Seeds

In the last decade, there has been interest worldwide concern about the antibacterial and&nbsp;antioxidant properties of plants. This work aims to study the antibacterial, antioxidant activities&nbsp;and chemical composition of Eucalyptus globulus essential oil leaves and seeds. Essential oil was extracted from Eucalyptus globulus seeds and leaves by hydrodistillation giving yields of 1.8% and 0.8% respectively, Chemical composition analysis by gas chromatography coupled with mass spectrometry (GC-MS) revealed the presence of a total of 33 compounds in seeds and 28 compounds in leaves. Antibacterial activity showed that essential oils was active against all tested bacterial strains. Antioxidant propriety measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) showed that essential oils obtained from leaves have higher antioxidant activity when compared to essential oils of seeds. However, the total antioxidant capacity revealed that seeds (22,68 mg EAA/g MS) have high activity with respect to leaves (5,44 mg EAA/g MS). These results confirmed the richness of the two essential oils in phenolic compounds responsible for their biological activity. A comparative bioclimatic study was carried out in order to investigate the effect of climate on chemical composition of Eucalyptus globulus leaves and seeds in Morocco. Factorial component analysis between chemical composition of essential oils from different countries and climatic parameters revealed that 1.8 cineole is strongly correlated with minimal (m) and maximal temperature values (M) and Emberger quotient (Q). Whereas, essential oils extracted from seeds is not affected by these parameters.&nbsp

Controle de fungos com óleo de eucalipto e transmissão de Fusarium sp. em sementes de Mimosa caesalpiniifolia

The objective of this work was to evaluate the effect of Eucalyptus globulus essential oil on the health and physiological quality of seeds of Mimosa caesalpiniifolia Benth. and the transmission of Fusarium sp. associated with seeds. The seeds were immersed in four concentrations of E. globulus essential oil (0.25%; 0.50%; 0.75% and 1.0%). The seeds were also treated with Captana fungicide (240g for 100 kg of seeds) and immersed in sterile distilled water (control). After being treated, the seeds were evaluated by the health test, using the filter paper method (blotter test) and by the physiological quality through germination, first count, germination speed index and seedling length. The transmission of Fusarium sp. via seeds was also assessed, as it is considered potentially pathogenic. The identified fungi were: Cladosporium sp. (16.5%), Aspergillus sp. (15.5%), Fusarium sp. (7.5%), Penicillium sp. (4.0%), Rhizopus sp. (2.5%) and Periconia sp. (0.5%). The essential oil of E. globulus, in any concentration, reduced efficiently the incidence of fungi associated with M. caesalpiniifolia seeds and did not affect the physiological quality of the treated seeds. Fusarium sp. was transmitted via seeds and caused root rot and hypocotyl necrosis.

Chemical Composition and Biological Activities of Eucalyptus globulus Essential Oil

Eucalyptus globulus essential oil (EGEO) is considered as a potential source of bioactive compounds with significant biological activity. The aim of this study was to analyze the chemical composition of EGEO, in vitro and in situ antimicrobial activity, antibiofilm activity, antioxidant activity, and insecticidal activity. The chemical composition was identified using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The main components of EGEO were 1,8-cineole (63.1%), p-cimene (7.7%), a-pinene (7.3%), and a-limonene (6.9%). Up to 99.2% of monoterpenes were present. The antioxidant potential of essential oil and results indicate that 10 μL of this sample can neutralize 55.44 ± 0.99% of ABTS•+, which is equivalent to 3.22 ± 0.01 TEAC. Antimicrobial activity was determined via two methods: disk diffusion and minimum inhibitory concentration. The best antimicrobial activity was shown against C. albicans (14.00 ± 1.00 mm) and microscopic fungi (11.00 ± 0.00 mm-12.33 ± 0.58 mm). The minimum inhibitory concentration showed the best results against C. tropicalis (MIC 50 2.93 µL/mL, MIC 90 3.17 µL/mL). The antibiofilm activity of EGEO against biofilm-forming P. flourescens was also confirmed in this study. The antimicrobial activity in situ, i.e., in the vapor phase, was significantly stronger than in the contact application. Insecticidal activity was also tested and at concentrations of 100%, 50%, and 25%; the EGEO killed 100% of O. lavaterae individuals. EGEO was comprehensively investigated in this study and information regarding the biological activities and chemical composition of the essential oil of Eucalyptus globulus was expanded.

Antibacterial Properties of Eucalyptus globulus Essential Oil against MRSA: A Systematic Review

Antimicrobial resistance is a huge threat against the public health sphere and is a major cause of global mortality and morbidity. Antibiotic misuse and overuse have led to the development of many resistant bacterial strains. One particular bacterium of concern is methicillin-resistant Staphylococcus aureus (MRSA), which is the most common resistant bacteria in humans. Antibiotic development has been unable to keep up with the rapid evolution of antibiotic-resistant organisms, and there is an urgent need to identify alternative agents to combat this problem. The purpose of this systematic review is to explore the literature on the antibacterial properties of Eucalyptus globulus essential oil against MRSA. The articles used in this review were obtained through a systematic search of the literature across four databases, with the timeline being between 2002 and 2022. Twenty studies were included in this review, which used various methods to investigate the antibacterial properties of E. globulus essential oil, alone or in combination with other agents, against MRSA. The findings suggest that E. globulus essential oil has antibacterial properties against MRSA, which can be enhanced when used in combination with other agents, such as other essential oils and antibiotics.

Low Concentrations of Eucalyptus Essential Oil Induce Age, Sex, and Mating Status-Dependent Stimulatory Responses in Drosophila suzukii

Plant-extracted essential oils are generally suggested as potential sources for alternatives to synthetic insecticides in insect pest control strategies. The increased interest in the use of essential oils derives from the generalized perception of their safety for the environment, human health, and non-target organisms as well as a lower risk of resistance development. However, studies on essential oils have largely focused on their activity on targeted insect pests while overlooking their potential unintended effects on insect biological and reproductive traits, especially with sublethal exposures. Here, we first determined the toxicity of Eucalyptus globulus essential oil to adults of Drosophila suzukii and assessed the effects of low concentrations (i.e., LC5 and LC20) in old (5–7 days) and mated flies. Subsequently, we assessed longevity and fecundity in newly emerged virgin flies from four couples’ combinations: unexposed couples, exposed females, exposed males, and exposed couples to the low concentration LC20. Our results show that eucalyptus essential oil has good insecticidal activity against adults of D. suzukii. However, compared to untreated flies, the exposure to low concentrations enhanced the females’ fecundity only when both old and mated female and male flies were exposed, while the females’ but not males’ life span was extended only in couples where newly emerged virgin females were exposed. Our findings suggest that although the eucalyptus essential oil may be a good control alternative for adult D. suzukii, its age-, sex-, and mating status-dependent stimulatory responses mediated by exposure to low concentrations need to be considered and further investigated.

Design Process and Economic Evaluation of an Innovative Healing Plaster in Gel made from Eucalyptus Globulus Essential Oil

The main goal of this work was to design a new product containing an active ingredient of the Eucalyptus Globulus tree - its essential oil. This work was divided into four steps: analysis of the raw material, chemical product design (needs, ideas, and product selection), manufacture, and economic analysis. After investigating the potential of all the ideas, the product selected was a plaster in gel, named Eucatrigel, with triple action: it protects the wound, accelerates the healing (due to the essential oil addition), and waterproofs the region. In the manufacturing step, it was defined the mass percentage of the essential oil in the gel as 0.5 %. The formula of the gel was based on a patent (US8563604B2) owned by Bausch Health Companies. A business case was set for the economic evaluation of this product; in this case, considering an initial investment of 681 k€, the expected payback period is four years, and the internal rate of return is 35 %.

Chemical Product Design of a Sports Performance T-shirt using Eucalyptus Globulus Essential Oil

This project consists of developing a Eucalyptus-based product. The selected product is a sports performance T-shirt impregnated with eucalyptus essential oil. A Chemical Product Design approach was used to translate customer needs into the new product. This procedure is divided into four stages: Needs, Ideas, Selection and Manufacture. This product’s target audience is athletics and hiking enthusiasts. Its main purpose is to valorize Eucalyptus Globulus essential oil’s unique properties, providing fresh and decongestion sensations to the user. Finally, an economic analysis was performed to evaluate the product viability.

Nanoemulsion and Nanogel Containing Eucalyptus globulus Essential Oil; Larvicidal Activity and Antibacterial Properties

Eucalyptus globulus essential oil (EGEO) possesses many biological effects such as antibacterial, antifungal, and insecticide properties. In the current study, the chemical composition of EGEO was first investigated using GC-MS analysis. Then, a nanoemulsion and nanogel containing EGEO (EGEO-nanoemulsion and EGEO-nanogel) were prepared. After that, the successful loading of EGEO was confirmed using ATR-FTIR analysis. EGEO-nanoemulsion and EGEO-nanogel with LC50 values of 27 and 32 μg/mL showed promising efficacies against Anopheles stephensi larvae. Besides, the efficacy of EGEO-nanogel (IC50 187 μg/mL) was significantly more potent than EGEO-nanoemulsion (IC50 3732 μg/mL) against Staphylococcus aureus. However, no significant difference was observed in the efficacy of EGEO-nanoemulsion and EGEO-nanogel against Pseudomonas aeruginosa. Natural components, straightforward preparation, and proper efficacy are some of the advantages of EGEO-nanogel; it could be considered for further consideration against other pathogens and mosquito larvae.

Eucalyptus oil nanoemulsions against eggs and larvae of Haemonchus contortus

Haemonchus contortus is a highly pathogenic and prevalent helminth that causes many deaths in sheep herds. Anthelmintics are usually employed to overcome this issue; however, they do not guarantee immediate and lasting efficacy because of the occurrence of drug-resistant parasites. Among substances that are used in scientific studies for parasitic control, essential oils are known to have different pharmacological properties. However, they demonstrate instability owing to several factors, and therefore, nanoemulsification is considered an alternative to control the instability and degradability of these compounds. The objective of this study was to evaluate the cytotoxicity of nanoemulsions containing essential oil of Eucalyptus globulus against the blood of healthy sheep and to verify their activity against the parasite H. contortus in sheep. The results presented adequate nanotechnological characteristics (diameter 72 nm, PDI 0.2, zeta −11 mV, and acidic pH) and adequate morphology. Further, the corona effect and cytotoxic profiles of the free oil and nanoemulsion against blood cells from healthy sheep were evaluated. The tests results did not present a toxicity profile. For evaluating efficacy, we observed an important anthelmintic action of the nanoemulsion containing oil in comparison to the free oil; the results demonstrate a potential role of the nanoemulsion in the inhibition of egg hatchability and the development of larvae L1 to L3 (infective stage). Based on these results, we developed an important and potential anthelmintic alternative for the control of the parasite H. contortus.

Development of a Natural Preservative System in Fluticasone Propionate Nasal Spray Formulation Using Eucalyptus globulus Essential Oil

Background: Replacing all or part of synthetic preservatives with natural antimicrobials in pharmaceutical formulations can be a way to improve the safety and quality of products. Evaluating the preservative effectiveness of essential oils is valuable since most of them are generally known to be safe. Objectives: In the current research, the efficacy of Eucalyptus globulus essential oil was investigated as an antimicrobial agent individually and in combination with benzalkonium chloride in fluticasone propionate nasal spray. Methods: To determine the minimum inhibitory concentration (MIC) of E. globulus essential oil, in vitro antimicrobial activity was assayed. The stability of nasal sprays was studied in intended preservation conditions at 40°C and RH: 25 %, and also the microbial challenge test was performed according to the United States Pharmacopoeia procedure, using reference strains: Staphylococcus aureus ATCC 1112, Escherichia coli ATCC 1330, Pseudomonas aeruginosa ATCC 1074, Candida albicans ATCC 5027, and Aspergillus brasiliensis ATCC 5011. Results: The combined application of E. globulus essential oil and benzalkonium chloride at a concentration of 0.675/0.005% v/v, in nasal spray formulation could decrease the bacterial and fungal populations, in compliance with United States Pharmacopoeia criteria, with considerable preservation within 28 days of the study compared to those preserved with only benzalkonium chloride (0.02%). Conclusions: In the current research, notable antimicrobial effectiveness of the combinational preservative system was observed and followed by a decrease in the functional level of the synthetic preservative. Therefore, after further studies, a combinational preservative system can be considered as an alternative preservative for pharmaceutical formulations.

Protective Effect of Some Essential Oils Against Gamma-Radiation Damages in Tetrahymena pyriformis Exposed to Cobalt-60 Source.

The main purpose of this study was to investigate the protective effect of Rosmarinus officinalis, Origanum compactum, Lavandula angustifolia, and Eucalyptus globulus essential oils (EOs) against gamma-radiation-induced damages on Tetrahymena pyriformis growing in presence of cobalt-60 source. The chemical composition of the 4 EOs was analyzed by gas chromatography-mass spectrometry. The protective effects of EOs on growth, on morphology, and on some metabolic enzymes and antioxidant markers have been evaluated. Thus, addition of EOs significantly improves the growth parameters (generation number and time) in irradiating conditions. All EOs allowed restoring growth parameters over more than 90% compared to the controls. The morphological analysis indicated that T. pyriformis cells growing in irradiating conditions were able to regain their normal form in presence of the different EOs. Our results indicate that the 4 EOs also have protective effects on some metabolic enzymes. They allowed recovering totally or partially the glyceraldehyde 3-phosphate dehydrogenase and the succinate dehydrogenase activities compared to the controls. Moreover, the addition of EOs reduced the lipid peroxidation level and decreased the activities of catalase and superoxide dismutase induced by the gamma-radiation exposure. A more pronounced protective effect was found for O. compactum and L. angustifolia EOs compared to R. officinalis and E. globulus EOs. These results suggest that the studied EOs are efficient natural antioxidants that could offer protection against gamma-radiation-induced damages and can therefore be useful in clinical medicine.

Efficiency of Ocimum basilicum and Eucalyptus globulus essential oils on anesthesia and histopathology of rainbow trout, Oncorhynchus mykiss

AbstractIn this study, anesthetic effects of Ocimum basilicum and Eucalyptus globulus essential oils were investigated on rainbow trout, Oncorhynchus mykiss. Fish (average 10 g) were exposed to 20, 50, 70, 100, 150, 200, 300, 400, 500, and 600 mg L−1 essential oils, anesthesia induction, and recovery times were recorded separately for each fish. Acute toxicities of essential oils (10 min LC50 concentration) on rainbow trout were determined at 70–400 mg L−1. In addition, histopathological effects of the essential oils in fish tissues were determined after deep anesthesia. As a result of this study, O. basilicum at 300 mg L−1 dose and E. globulus essential oils at 400 mg L−1 dose showed ideal anesthetic effects on rainbow trout. Anesthesia induction (stage 4) and recovery times at this concentration for E. globulus were found as 186 and 117.5 s, respectively. Anesthesia induction (stage 4) and recovery times were 220.5 and 61 s for this concentration in O. basilicum essential oil, respectively. The toxic effects of essential oils for rainbow trout were not found. No pathological finding in gill, liver, and kidney was observed at the histopathological examination. Consequently, this study demonstrated that O. basilicum and E. globulus essential oils can be used as an effective and safe anesthetic in rainbow trout.