Red Blood Cell Erythrocyte Research Articles

Influence of the NAT2 gene polymorphic markers on the effectiveness and safety of treatment in patients with newly diagnosed pulmonary tuberculosis based on peripheral red blood cell dynamics

Background. Individual sensitivity to isoniazid in tuberculosis patients is determined by the presence of N-acetyltransferase 2 (NAT2) enzyme gene allelic variants in genome. Evaluation of quantitative and qualitative alterations in peripheral blood can be used for diagnosis, disease severity estimation, or as a clue for estimation of anti-tuberculosis chemotherapy effectiveness and safety.Aim: Find associations between acetylation type and peripheral red blood cell (RBC) dynamics; determine the effect of NAT2 acetylation rate on the effectiveness and safety of treatment in patients with newly identified pulmonary tuberculosis (TB) residing in the Sakha Republic (Yakutia).Methods. This study included 146 patients with various clinical forms of newly diagnosed pulmonary TB. Oral isoniazid, rifampicin, pyrazinamide, and ethambutol were administered patients. Genotyping was performed via real time PCR.Results. Rapid and intermediate acetylators showed an increase in hemoglobin concentrations and RBC erythrocyte hemoglobin content by the end of chemotherapy (P<0.05). Incidence of anemia was lower in intermediate acetylators, compared to rapid or slow acetylators (P=0.013). Negative correlation was established between absolute RBC count and slow acetylation type (P=0.017). Patients with rapid acetylation type showed increased RBC distribution width indexes RDW-CV and RDW-SD (P<0.05).Conclusions. An adequate therapeutic effect was achieved with standard doses of anti-TB medications in patients with intermediate acetylation type. Rapid and slow acetylators required anti-TB medication dose correction. Genotyping for NAT2 gene in patients with pulmonary TB enables clinicians to choose the optimal dose of anti-TB medications, specifically, isoniazid dose.

Lithium erythrocyte:Plasma ratio in primary affective disorder

The purpose of this paper is to discuss the metabolism of the lithium ion, particularly the erythrocyte-plasma lithium ratio, in relation to clinical studies of affective disorder. The chronic administration of lithium salts has been shown to be effective in the long-term management of patients with recurrent affective illness. However, the mechanism of action of lithium ion relating to its therapeutic efficacy is unknown. Recent studies of lithium ion metabolism have focused on the ratio of lithium ion in erythrocytes to plasma. 1–5 The erythrocyte red blood cell (RBC) is used as a model for intracellular lithium metabolism, and the ratio of lithium ion concentration in RBCs to plasma has been shown to be under genetic control. 4,6 Furthermore, it has been reported that patients who demonstrate an antidepressant response to lithium administration have higher RBC-plasma lithium ratios, although not all studies agree. 1,3,5 Also, studies have suggested that women with affective disorder may have higher RBC-plasma lithium ratios than women controls or males with manic depressive illness. 2

Haematological Profile Of Thai And Indigenous Male And Female Air Breathing Climbing Perch

This study evaluated the haematological profile of air breathing Thai and indigenous male and female climbing perch (in Bangla, koi) Anabus testudineus to improve understanding the differences between two strains at blood parameters level. Haemoglobin (Hb), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), erythrocyte red blood cells (RBC) or erythrocytes, lymphocytes, monocytes, neutrophils and eosinophils were identified by mythic 18 automated haematology analyzer. Most haematological parameters indicated significant (p < 0.05) intra species sex variations. Higher Hb concentration, RBC counts and HCT percentages were found in Thai and indigenous males compared to their counterparts but higher MCH values, WBC counts and ESR levels were detected in both Thai and indigenous females than in the males. Similar concentration of Hb was detected in the same sex between fish. In contrast, higher level of ESR was observed in both female than did the males. Total counts of RBC observed in both Thai and indigenous male was significantly higher than in the females. MCV was found higher in indigenous male and female than in Thai one. However, no significant differences were observed in neutrophils, lymphocytes and eosinophils. Findings of this study showed sex variations in the haematological parameters between Thai and indigenous climbing perch. DOI: http://dx.doi.org/10.3329/dujbs.v21i1.9746 DUJBS 2012 21(1): 67-77