Erugosquilla Massavensis Research Articles

Growth, mortality, and exploitation rates of the mantis shrimp Erugosquilla massavensis (Kossman, 1880) (Malacostraca: Stomatopoda) in Iskenderun Bay, north-eastern Mediterranean of Türkiye

ABSTRACT Lessepsian Erugosquilla massavensis is one of the three Stomatopod species reported on the Levantine coast of Türkiye. Its natural habitat is the Red Sea and it is the first Red Sea stomatopod species reported from the Mediterranean. This study aims to determine the morphometric relationships, growth parameters, sex ratio, age, mortality, and exploitation rates of non-native E. massavensis in the north-eastern Mediterranean. Samples were collected monthly by commercial trawl surveys between September 2016 and March 2017 in Iskenderun Bay. Relationships between the carapace length (CL)–total length (TL) and the total length (TL)–total weight (TW) were estimated. Negative allometric growth was observed between the CL–TL and TL–TW relationship for females, males, and all. The von Bertalanffy growth parameters (vBGP) were found L ∞ = 155.4 mm, K = 0.520 year−1, t 0 = −0.199, ø′ = 4.099 for females, L ∞ = 157.5 mm, K = 0.600 year−1, t 0 = −0.127, ø′ = 4.173 for males. This research provided the first-ever data on the vBGP of E. massavensis. The study found that both sexes had a higher fisheries mortality coefficient than natural mortality (Z = 2.01, M = 0.71, F = 1.3) and were exploited (E = 0.65).

Biodiversity of macro-benthic invertebrates in the Eastern Egyptian Mediterranean coast

The present research paper aims to study the biodiversity of macro-benthic invertebrates caught by bottom trawl nets along the eastern 180 km of the Egyptian Mediterranean coast. A total of 21 hauls were collected seasonally from July 2017 to May 2018. A total of 10765 individuals representing 118 taxa were collected during the study, affiliated with eight phyla. The maximum diversity (70 taxa) was recorded in spring; meanwhile, the highest abundance (3856 individuals) was registered in winter. Mollusca was the most diverse phylum with 46 species, while Arthropoda was the most dominant, constituting 87.89% of the total amount of benthos. The stomatopod Erugosquilla massavensis was the most prevalent species (about 41.8% of total benthos). Of the 118 recorded taxa, 11 species were regarded as new records in the Egyptian Mediterranean Sea. The maximum diversity index value (Hˊ=2.81) was estimated for haul 10 (autumn); meanwhile, a minimum value of (0.26) was estimated for haul 15 (winter). Excluding the 10 taxa identified at the genus level, there were 71 indigenous species (66%). In contrast, alien, or non-indigenous species (NIS) contributed a relatively high number (37 species), constituting 34% of the total number of identified species. Additionally, 24.2% were of Indo-Pacific and Indian origin. So, planned comprehensive national monitoring programs for biodiversity and NIS in the study area are recommended for recording any new alien species.

Fabrication and assessment of potent anticancer nanoconjugates from chitosan nanoparticles, curcumin, and eugenol.

In cancer management and control, the most challenging difficulties are the complications resulting from customized therapies. The constitution of bioactive anticancer nanoconjugates from natural derivatives, e.g., chitosan (Ct), curcumin (Cur), and eugenol (Eug), was investigated for potential alternatives to cancer cells' treatment. Ct was extracted from Erugosquilla massavensis (mantis shrimp); then, Ct nanoparticles (NCt) was fabricated and loaded with Cur and/or Eug using crosslinking emulsion/ionic-gelation protocol and evaluated as anticancer composites against CaCo2 "colorectal adenocarcinoma" and MCF7 "breast adenocarcinoma" cells. Ct had 42.6kDa molecular weight and 90.7% deacetylation percentage. The conjugation of fabricated molecules/composites and their interactions were validated via infrared analysis. The generated nanoparticles (NCt, NCt/Cur, NCt/Eug, and NCt/Cur/Eug composites) had mean particle size diameters of 268.5, 314.9, 296.4, and 364.7nm, respectively; the entire nanoparticles carried positive charges nearby ≥30mV. The scanning imaging of synthesized nanoconjugates (NCt/Cur, NCt/Eug, and NCt/Cur/Eug) emphasized their homogenous distributions and spherical shapes. The cytotoxic assessments of composited nanoconjugates using the MTT assay, toward CaCo2 and MCF7 cells, revealed elevated anti-proliferative and dose-dependent activities of all nanocomposites against treated cells. The combined nanocomposites (NCt/Eug/Cur) emphasized the highest activity against CaCo2 cells (IC50 = 11.13μg/ml), followed by Cur/Eug then NCt/Cur. The exposure of CaCo2 cells to the nanocomposites exhibited serious DNA damages and fragmentation in exposed cancerous cells using the comet assay; the NCt/Eug/Cur nanocomposite was the most forceful with 9.54nm tail length and 77.94 tail moment. The anticancer effectuality of innovatively combined NCt/Cur/Eug nanocomposites is greatly recommended for such biosafe, natural, biocompatible, and powerful anticancer materials, especially for combating colorectal adenocarcinoma cells, with elevated applicability, efficiency, and biosafety.

Biometric study, sex ratio and potential biological activities of the edible mantis shrimp Erugosquilla massavensis

Biometric study, sex ratio and potential biological activities of the edible mantis shrimp Erugosquilla massavensis

Beneficial Bioapplications of Silver Nanoparticles Synthesized by a Marine Crustacean (Erugosquilla massavensis)

Beneficial Bioapplications of Silver Nanoparticles Synthesized by a Marine Crustacean (Erugosquilla massavensis)

Molecular identification and phylogenetic relationship of Erugosquilla massavensis (Kossmann, 1880) from the Mediterranean Sea, Egypt

The Erythraean species of mantis shrimp, Erugosquilla massavensis, has been reported as the first Lessepsian migrant stomatopod to the Mediterranean Sea in 1933, off Alexandria, Egypt via the Suez Canal. Currently, it extended to the middle and western Mediterranean competing with the native spot-tail mantis shrimp Squilla mantis (Linnaeus, 1758). The continuous increase of invasive species to the Mediterranean claimed the necessity to update and revise the identification of those early migrants. The present study aimed to confirm the morphological identification of E. massavensis from the coast of the Egyptian Mediterranean Sea using the DNA barcoding gene cytochrome oxidase subunit I (COI) and conducting a phylogenetic analysis. Genomic DNA of samples collected from the Mediterranean Sea at Port Said in 2016 was extracted and COI was amplified and sequenced. Our phylogenetic analysis revealed that E. massavensis (MH447072 and MH447073) constitutes a single monophyletic clade and appeared more linked with E. woodmasoni than the Japanese mantis shrimp Oratosquilla oratoria (divergence value < 3%). The phylogenetic relationship between E. massavensis, E. woodmasoni, and O. oratoria was confirmed by the Neighbor-Joining tree. The present study confirmed the morphological identification and document, for the first time in GenBank/EMBL/ DDBJ genetic databases, the genetic status of E. massavensis from the Mediterranean of Egypt using COI.

First record of the Red Sea Mantis shrimp Erugosquilla massavensis (Kossmann, 1880) in the Greek Ionian Sea

The presence of the Red Sea Mantis shrimp Erugosquilla massavensis (Kossmann, 1880) is here reported for the first time from the southeastern Ionian Sea (Zakynthos Island, Greece). This record is the first evidence of the presence of a Lessepsian migrant crustacean in the aforementioned area while it fills the gap in the ongoing westward and northward distribution range expansion of this wide spread invader of the Mediterranean basin.

Analyses of the non-target catch from the Egyptian Mediterranean trawlers, off Port Said

The present study aimed to analyze the non-target catch in the Egyptian Mediterranean waters, off Port Said, during the period from spring 2017 to winter 2018. The analysis revealed that from 75 non-target species, fish constituted about 69.4% of the total catch weight, whereas invertebrates reached about 30.6%. For non-target fish, 51 fishes were identified (50 bony fishes and one elasmobranch), of which 17 lessepsian species were found. The high percentage of fish (82.67%) was with an average length between 4.0 and <9.0 cm. The highly represented fish species by numbers were: Engraulis encrasicolus (25.608%), Herklotsichthys punctatus (23.711%), Terapon puta (9.572%), Equulites klunzingeri (9.342%), Gobius niger (7.177%), Argyrosomus regius (6.280%), Alepes djedaba (4.155%), Gobius paganellus (3.397%), Sardinella aurita (2.525%), Ariosoma balearicum (1.093%) and Diplodus annularis (0.803%). Total analysis, seasonal analysis and an index of diversity (H′) for the non-target catch species were provided. On the other hand, length-weight relationship, condition factor and age composition were described for the most dominant species. For non-target invertebrates, 24 species were identified among which 13 lessepsian species were found. Shrimps and mantis shrimp Erugosquilla massavensis were the main constituents during the period of study. The authors propose to change the cod end mesh size from 2.5 cm to 4.0 cm to minimize the high percentage of non-target catch and preserve recruits of valuable species from over fishing along the Egyptian coast of the Mediterranean Sea while doing experiments to ensure the best results. Further, continuous monitoring and managements of trawlers must be put into consideration.

Nutritive value and safety aspects of acidified mantis shrimp during ambient storage

In this study effects of acidification with an organic acid (3 %, formic acid - FA) and an organic-inorganic acid mixture (1.5 % FA + 1.5 % sulphuric acid - FASA) were evaluated on a non-target species (mantis shrimp - Erugosquilla massavensis). Nutritional composition (proximate analysis and fatty acid composition), chemical (biogenic amine concentrations, non-protein nitrogen -NPN and pH) and microbiological assessments (total viable counts -TVC and lactic acid bacteria counts -LAB) were conducted under 27-28 °C. The analysis conducted for a 60 day period at 1st, 7th, 14th, 30th, 42nd and 60th day intervals. Moisture contents of acidified shrimp were significantly lower than the raw material. No changes in protein contents and an increase in lipid content only in FASA group were observed. Palmitic acid, stearic acid, palmitoleic acid, oleic acid and docosahexaenoic acid (DHA) were observed to be the dominant fatty acids in raw and acidified mantis shrimp. The initial pH value of mantis shrimp was 7.71 and became stable (4.14-3.97) throughout the storage period. An increase was observed in NPN contents and FA and FASA were 0.56 and 0.51 g 100 g-1, respectively at the end of the storage. Putrescine (3.00 mg 100 g-1), tyramine (2.94 mg 100 g-1) and serotonin (2.71 mg 100 g-1) were found to be the dominant biogenic amines in raw mantis shrimp. No significant changes in biogenic amine concentrations were observed in general during the storage period. TVC was found as 4.16 log cfu g-1 at the beginning of the storage period. Bacterial load was decreased after the addition of acids and stayed low throughout the storage period. Increases were observed in LAB and this value were 4.50 and 5.68 log cfu g-1 for FA and FASA group, respectively at the end of the storage period. The results showed acid treated mantis shrimp could be considered potential feed component due to its high nutritional value and safe in regards of biogenic amines.

Much can change in a year: the Massawan mantis shrimp, Erugosquilla massavensis (Kossmann, 1880) in Sicily, Italy

Much can change in a year: the Massawan mantis shrimp, Erugosquilla massavensis (Kossmann, 1880) in Sicily, Italy

Assessment of antioxidant, antimicrobial and anticancer activities of carotenoid extracted from Erugosquilla massavensis and Procambarus clarkii exoskeletons

The present study was carried out on both marine and fresh water edible crustaceans namely; Erugosquilla massavensis and Procambarus clarkii, respectively, which are predominant in the local aquatic fauna in Egypt. The present study was conducted to determine the total antioxidant capacity (TAC), total phenolic, total flavonoid and carotenoid content in muscle and exoskeleton of E. massavensis and P. clarkii crustaceans. The study also evaluate the antioxidant, antimicrobial and antitumor activities of the carotenoid extracted from these species. The results showed that TAC, phenolic and flavonoid contents in both muscle and exoskeleton of E. massavensis are higher than those contents in P. clarkii. The results showed also that the carotenoid content extracted from the males and females exoskeleton of E. massavensis are higher than those of males and females of P. clarkii. The antioxidant activity of carotenoid extracted from male and female of P. clarkii showed higher activity than those extracted from E. massavensis. By contrast, carotenoids extracted from females and males of E. massavensis showed higher antimicrobial activity than those of females and males P. clarkii. The carotenoid extract shows relatively weak antitumor activity in vitro against the Earlich cell lines. In conclusion, both of E. massavensis and P. clarkii have a bioactive compound with antimicrobial and antioxidant activities.

First occurrence of Erugosquilla massavensis (Kossmann, 1880) in Italian waters (Ionian Sea)

First occurrence of Erugosquilla massavensis (Kossmann, 1880) in Italian waters (Ionian Sea)

Heavy Metals Monitoring Using Commercially Important Crustacean and Mollusks collected from Egyptian and Saudi Arabia Coasts

The objectives of this study was to determine and compare the concentration levels of five selective essential trace metals ( Fe, Cu, Zn, Co and Se) and three non-essential metals (Hg, Pb and Cd) in the edible muscle tissues of commercially important species of male and female crustaceans (Erugosquilla massavensis, Penaeus semisulcatus, Metapenaeus monoceros, Portunus pelagicus) and mollusks (Sepia spp. And Cardium edule). It was carried out also to evaluate the bioaccumulation process of the elements based on the Metal Pollution Index (MPI) as an attempt to use these organisms as bioindicators of pollution of Mediterranean Sea, Red Sea and the Arabian gulf and to ensure the seafood safety from these regions. The obtained results revealed that significant variations of Fe, Cu, Zn, Co, Hg, Pb and Cd levels in edible muscles of crustacean and mollusk organisms were observed at different localities surveyed, as well as between sex of each species and environmental areas. The present data also, show that MPI factor of essential metals were higher than that of non-essential heavy metals in all studied organisms. Moreover, MPI values suggested that mantis shrimps E. massavensis and bivalve mollusks C. edule have a greater capacity for metal bioaccumulation than shrimps, crab and cephalopod mollusks, so E. massavensis and C. edule are more vulnerable to metal pollution than the other studied species. Therefore, it is suggested that E. massavensis and C. edule can be used as bioindicators of metal pollution

Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs

Crustaceans and mollusks are important components of the aquatic fauna. Furthermore, crustaceans and mollusks consumption have been increasing worldwide during the past decades. So, therefore, the present study was investigated the variation between total protein content and protein electrophoretic pattern in edible muscle of commercially important crustaceans (Erugosquilla massavensis, Peaneus semisulcatus, Metapenaeus monoceros and Portunus pelagicus) and mollusks (Sepia spp., Cardium edule) as an attempt to rank them as an alternative rich animal protein source for human. The recorded data declare that the highest protein percentage was observed in males E. massavensis followed by Sepia spp then P. pelagicus and shrimps. On the other hand, females E. massavensis and bivalve mollusks (C. edule) had less total protein contents in their muscles, additionally, the recorded data showed a variation in band numbers and estimated molecular weight of muscle protein between sexes of each species and among the studied species. The results of the present work clearly indicate that there are differences in the protein structure of the muscles in different species of crustaceans and mollusks with reference to the protein fractions and their molecular weights.

Tagmatization in Stomatopoda – reconsidering functional units of modern-day mantis shrimps (Verunipeltata, Hoplocarida) and implications for the interpretation of fossils

IntroductionWe describe the tagmatization pattern of the anterior region of the extant stomatopod Erugosquilla massavensis. For documentation we used the autofluorescence capacities of the specimens, resulting in a significant contrast between sclerotized and membranous areas.ResultsThe anterior body region of E. massavensis can be grouped into three tagmata. Tagma I, the sensorial unit, comprises the segments of the eyes, antennules and antennae. This unit is set-off anteriorly from the posterior head region. Ventrally this unit surrounds a large medial sclerite, interpreted as the anterior part of the hypostome. Dorsally the antennular and antennal segments each bear a well-developed tergite. The dorsal shield is part of tagma II, most of the ventral part of which is occupied in the midline by the large, partly sclerotized posterior part of a complex combining hypostome and labrum. Tagma II includes three more segments behind the labrum, the mandibular, maxillulary and maxillary segments. Tagma III includes the maxillipedal segments, bearing five pairs of sub-chelate appendages. The dorsal sclerite of the first of these tagma-III segments, the segment of the first maxillipeds, is not included in the shield, so this segment is not part of tagma II as generally thought. The second and third segments of tagma III form a unit dorsally and ventrally. The tergites of the segments of tagma III become progressively larger from the anterior to the posterior, possibly resulting from a paedomorphic effect during evolution, which caused this reversed enlargement.ConclusionsThe described pattern of tagmosis differs from current textbook knowledge. Therefore, our re-description of the anterior body area of stomatopods is of considerable impact for understanding the head evolution of Stomatopoda. Likewise, it has a bearing upon any comparisons with fossil stomatopods, as mainly sclerotized areas are fossilized, and, on a wider scale, upon larger-scale comparisons with other malacostracans and eucrustaceans in general.

English

Both fresh and marine edible crustaceans Procambarus clarkii and Erugosquilla massavensis, respectively, are now important components of our local aquatic fauna in Egypt that have a small yet growing economic importance in our markets. So, the purpose of the present work was, therefore, to assess the protein, amino acids, fatty acids, minerals, vitamins, protein electrophoresis and dendrogram analysis of their extracts for the first time in Egypt which may be in the future play an important role in some pharmaceutical industries and may be used as a specific health foods (functional supplement). In the present study, nutritional analysis of muscle and exoskeleton extracts of P. clarkii and E. massavensisindicated the presence of a high amount of protein, 9 essential amino acids and 9 non essential amino acids including taurine which has a very scientifical action and benefit for men. Also, the results showed the presence of 9 unsaturated fatty acids which included essential fatty acids linoleic acid (Omega 6) and alpha linolenic acid (Omega 3) which have many functions for man (food supplement) in addition to 4 saturated fatty acids. The minerals recorded were Cu, Zn, Mn, Fe, Mg, Ca, K and P and the vitamins were Vitamims A, D, E, B1, B2, Bp, B6 and B12. From the electrophoretic analysis of protein, the dendrogram analysis showed the highest similarity between the muscle and exoskeleton extracts of P. clarkii. Key words: Procambarus clarkii, Erugosquilla massavensis, extracts, total protein, amino acids, fatty acids, minerals, vitamins, protein electrophoresis, dendrogram analysis.

Expanding distribution and occurence of the Indo-Pacific Stomatopod, Erugosquilla massavensis (Kossmann, 1880) on the Aegean coast of Turkey

The indo-Pacific mantis shrimp, Erugosquilla massavensis was recently collected from Sigacik Bay,located on the Aegean coast of Turkey. It is the first record of the species along the Aegean coast of Turkey

First Mediterranean record of the Indo-West Pacific mantis shrimp, Clorida albolitura Ahyong &amp; Naiyanetr, 2000 (Stomatopoda, Squillidae)

To date, only one species of Erythrean stomatopod, Erugosquilla massavensis (Kossmann), has been reported from the Mediterranean Sea. We report the first appearance of a second Erythrean stomatopod species from the Mediterranean coast of Israel, Clorida albolitura Ahyong & Naiyanetr, on 24 May 2006.