Ergodic Research Articles

Feed-induced hypersalivation in horses from Austria, Germany and Switzerland.

While previous reports come mostly from the southern Americas, several outbreaks of hypersalivation in horses were observed in Middle Europe from 2016 to 2018. To describe feed-induced hypersalivation in European horses. Analysis of feedstuffs. Veterinarians and horse or stable owners were encouraged to submit feedstuffs from case outbreaks of hypersalivation in which, infectious diseases or other systemic causes of the syndrome were ruled out and intoxication was suspected. Feedstuff analysis was performed, including gross examination, microscopic analysis of fine particles, and for hay and forage mycological culturing and mycotoxin testing. Eleven case outbreaks were investigated. Typical clinical findings in the horses were either foamy saliva around the mouth or serous salivation with puddles forming on the ground. Some horses also showed lesions of the gingival mucosa and/or the tongue. Foamy hypersalivation, sometimes combined with lesions of tongue and/or gingiva, was associated with finding of ergot sclerotia and ergot alkaloids in hay or pasture plants (ergocornin and ergocorninin having the highest concentrations). Serous hypersalivation with massive loss of fluid was associated with the fungus Rhizoctonia spp. As indicated by the detection of traces of swainsonine, mycotoxin production on the forage was seen as likely cause. The cessation of clinical signs took days to weeks after diet change, probably depending on the duration, type and amount of toxin intake. Small number of case outbreaks, records of clinical findings and horses' management were incomplete and were reported by horse owners. Hypersalivation due to mycotoxins in the feed has become an emerging problem for horses in middle Europe.

Optimization, Validation, and Application of Cleanup-Coupled Liquid Chromatography–Tandem Mass Spectrometry for the Simultaneous Analyses of 35 Mycotoxins and Their Derivatives in Cereals

Mycotoxins occur singly or as co-contaminants and are primarily present in carbohydrate-rich foods such as cereals and cereal-based products. To effectively monitor mycotoxin co-contamination in cereals and cereal-based products, the simultaneous analysis of mycotoxins and their derivatives is required. Therefore, we coupled cleanup with LC-MS/MS for the rapid and robust quantitation of 35 analytes in wheat samples, including ergot alkaloids (EAs), which are rarely included in such analyses. To investigate the effects of different mycotoxin types on adsorbents, various dispersive solid-phase extraction sorbents were evaluated; a C18 end-capped sorbent exhibited the most effective cleanup performance. The method was validated by analyzing samples fortified with the mycotoxins at three concentration levels. The results exhibited high linearity, high recoveries, and repeatability. The methodology was applied for commercial cereal samples. The cereal samples were found to be 74% contaminated, and two samples measured levels of EAs at 609.63 μg/kg and 294.93 μg/kg, exceeding the limits defined by the EU for rye milling products. These findings highlight the validity of our novel method and the necessity of continuously monitoring mycotoxin levels in cereals to ensure food safety.

Polysubstituted Pyridines from 1,4-Oxazinone Precursors.

This study describes a general method for the preparation of 1,4-oxazin-2-one intermediates from acetylene dicarboxylate and β-amino alcohol precursors. Oxazinones prepared in this manner were employed in a tandem cycloaddition/cycloreversion reaction sequence with a model alkyne (phenyl acetylene) to give substituted pyridine products. Fundamental reactivity and selectivity studies are complemented by the synthesis of the polycyclic ergot alkaloid natural product xylanigripone A.

Ergot alkaloids: comparison of extraction efficiencies for their monitoring in several cereal-solvent combinations by UPLC-MS/MS.

To date, there are more than 80 ergot alkaloids identified; their distribution depends on different factors (e.g. geographic regions, host plants). These toxins can cause acute and chronic toxic effects on human health and commonly infect cereal crops such as triticale and rye, wheat, barley and oats. Considering the growing consumption of plant-based foods, the European Food Safety Authority has highlighted the need to develop risk assessment strategies. This work focused on the optimization of extraction efficiency, to quantify the main ergot alkaloids and their epimers, that are available on the market without any legal restriction (ergosine, ergocristine, ergocriptyne, ergocornine, ergosinine, ergocristinine, ergocriptinine and ergocorninine). Considering the quantification of 8 out of 12 regulated compounds by EU (sum of -ine and -inine forms), this approach can be defined as a screening method for a reliable estimation of the risk, specifically devoted to industrial stakeholders that can then possibly outsource to authorized external labs only the samples suspected of significant positivity. The effectiveness of three different extraction conditions (acidic, alkaline and neutral) followed by a rapid clean-up using dispersive solid-phase extraction with C18 sorbent was evaluated by ultra-performance liquid chromatography tandem quadrupole mass spectrometry (UPLC-MS/MS), resulting in a short chromatographic run (16min). The method was developed and validated in five different cereal production chains (rye, oat, wheat, wheat gluten and baby food). The applicability of the method was examined by analyzing a set of 54 samples, including also other cereals like spelt, tritordeum and triticale, and evaluating also some reference materials.

Recent Advances in Mycotoxin Detection: A Review

Mycotoxins, toxic compounds produced by fungi like Aspergillus and Penicillium, pose significant health risks even at low concentrations. Detecting these contaminants in food requires intricate methods due to their low levels and complex sample matrices. Historically, toxic fungi research began with mushrooms but expanded in the mid-1800s to include other fungi, such as Claviceps purpurea, which caused ergotism through contaminated rye. Mycotoxins severely impact both human and animal health, causing issues like cancer, immune suppression, and nervous system damage, while also disrupting global food trade, affecting around 25% of crops worldwide. Traditional detection methods include chromatography (TLC, LC, GC, HPLC) and immunological assays like ELISA and LFI, though these methods have limitations. Recent advances in mycotoxin detection feature innovations like biosensors, fluorescence-based techniques, phage display technology, and smartphone-enabled systems, which offer enhanced sensitivity, specificity, and rapid detection capabilities. These methods represent a promising shift towards more efficient and precise mycotoxin analysis in food safety.

Contaminants and residues have varied distributions in large volumes of wheat

Analysis of bulk wheat consignments for naturally-occurring contaminants and residues from plant protection products is common, and helps manage potential health risks to consumers. The heterogeneous distribution of some mycotoxins in wheat has been described, however the distribution of other contaminants and residues has not yet been reported. This study characterized distributions of deoxynivalenol, ochratoxin A, ergot alkaloids, cadmium, and glyphosate in nine large consignments of wheat by analysing composite samples representing sub-lots prepared from increments obtained during the entire loading process. The widest span of concentrations within a consignment occurred for ochratoxin A (<0.5–22.9 µg/kg) and ergot alkaloids (0.009–0.486 mg/kg), followed by deoxynivalenol (<0.05–0.76 mg/kg) and glyphosate (<0.3–5.01 mg/kg), and then cadmium (0.022–0.102 mg/kg). Experimental semivariograms were plotted to model the spatio-temporal correlation of analytes within consignments during loading. Analyses demonstrated that distributions of contaminants and residues within a particular consignment differed, and that distributions of a particular contaminant or residue differed among consignments. The results indicate that sampling during only a portion of a loading or unloading process can result in a composite sample that is not representative of the consignment and thus increase the risk of misclassifying a consignment as compliant.

An Alternative Method of Reversed-Phase HPLC for Identification and Quantitative Determination 0f Ergot Alkaloids

An Alternative Method of Reversed-Phase HPLC for Identification and Quantitative Determination 0f Ergot Alkaloids

On the linear response theory of vortex meandering and its statistical verification in experiments

Meandering designates the main manifestation of unsteady vortex dynamics observed in experiments. This study has the twofold objective to (i) develop a theoretical model describing vortex meandering and (ii) conduct a quantitative and objective evaluation of the model against experimental data. Based on an analogy with Brownian motion, we derive the theoretical model in the framework of linear response theory. Taking the form of a Langevin equation, our model explains meandering as the competition between external excitation by free-stream perturbations, counteracted by stabilising intrinsic vortex dynamics. As such, it contains the previous approaches to explaining the phenomenon as limiting cases, and clearly highlights their shortcomings. The statistical identification of characteristic regularities in experimental data as well as the assessment of their consistency with theoretical models are important problems in physics. For samples obtained from finite-length records of correlated data, these statistical characteristics are not unique and may show spurious behaviour merely induced by the finiteness of the sample. Statistical inference provides a systematic and quantitative methodology to objectively assess the reproducibility of statistical characteristics and to evaluate their consistency with theoretical models. Their systematic application to the analysis of vortex meandering has not been done before and provides statistical evidence for our proposed Brownian-motion-like model. That is, experimental vortex meandering constitutes the manifestation of a stationary Gauss–Markov random process, which implies that the dynamics admits an ergodic probability measure.

Ergot alkaloid control in biotechnological processes and pharmaceuticals (a mini review).

The control of ergot alkaloids in biotechnological processes is important in the context of obtaining new strain producers and studying the mechanisms of the biosynthesis, accumulation and secretion of alkaloids and the manufacturing of alkaloids. In pharmaceuticals, it is important to analyze the purity of raw materials, especially those capable of racemization, quality control of dosage forms and bulk drugs, stability during storage, etc. This review describes the methods used for qualitative and quantitative chemical analysis of ergot alkaloids in tablets and pharmaceutic forms, liquid cultural media and mycelia from submerged cultures of ergot and other organisms producing ergoalkaloid, sclerotias of industrial Claviceps spp. parasitic strains. We reviewed analytical approaches for the determination of ergopeptines (including their dihydro- and bromine derivatives) and semisynthetic ergot-derived medicines such as cabergoline, necergoline and pergolide, including precursors for their synthesis. Over the last few decades, strategies and approaches for the analysis of ergoalkaloids for medical use have changed, but the general principles and objectives have remained the same as before. These changes are related to the development of new genetically improved strains producing ergoalkaloids and the development of technologies for the online control of biotechnological processes and pharmaceutical manufacturing ("process analytical technologies," PAT). Overall, the industry is moving toward "smart manufacturing." The development of approaches to production cost estimation and product quality management, manufacturing management, increasing profitability and reducing the negative impact on personnel and the environment are integral components of sustainable development. Analytical approaches for the analysis of ergot alkaloids in pharmaceutical raw materials should have high enough specificity for the separation of dihydro derivatives, enantiomers and R-S epimers of alkaloids, but low values of the quantitative detection limit are less frequently needed. In terms of methodology, detection methods based on mass spectrometry have become more developed and widespread, but NMR analysis remains in demand because of its high accuracy and specificity. Both rapid methods and liquid chromatography remain in demand in routine practice, with rapid analysis evolving toward higher accuracy owing to improved analytical performance and new equipment. New composite electrochemical sensors (including disposable sensors) have demonstrated potential for real-time process control.

Directional Pinsker algebra and its applications

In this paper, we introduce the directional Pinsker algebra, and construct a skew product to study it. As applications, we show that (i) if a Z2-system with positive directional measure-theoretic entropy then it is multivariant directional mean Li–Yorke chaotic along the corresponding direction; (ii) for any ergodic measure on a Z2-system, the intersection of the set of directional measure-theoretic entropy tuples with the set of directional asymptotic tuples is dense in the set of directional measure-theoretic entropy tuples.

Rank-one non-singular actions of countable groups and their odometer factors

Abstract For an arbitrary countable discrete infinite group G, non-singular rank-one actions are introduced. It is shown that the class of non-singular rank-one actions coincides with the class of non-singular $(C,F)$ -actions. Given a decreasing sequence of cofinite subgroups in G with $\bigcap _{n=1}^\infty \bigcap _{g\in G}g\Gamma _ng^{-1}=\{1_G\}$ , the projective limit of the homogeneous G-spaces $G/\Gamma _n$ as $n\to \infty $ is a G-space. Endowing this G-space with an ergodic non-singular non-atomic measure, we obtain a dynamical system which is called a non-singular odometer. Necessary and sufficient conditions are found for a rank-one non-singular G-action to have a finite factor and a non-singular odometer factor in terms of the underlying $(C,F)$ -parameters. Similar conditions are also found for a rank-one non-singular G-action to be isomorphic to an odometer. Minimal Radon uniquely ergodic locally compact Cantor models are constructed for the non-singular rank-one extensions of odometers. Several concrete examples are constructed and several facts are proved that illustrate a sharp difference of the non-singular non-commutative case from the classical finite measure preserving one: odometer actions which are not of rank-one and factors of rank-one systems which are not of rank one; however, each probability preserving odometer is a factor of an infinite measure preserving rank-one system, etc.

A gene regulating ergot alkaloid biosynthesis in Metarhizium brunneum.

Ergot alkaloids produced by several species of fungi are important as contaminants of food and feed in agriculture and also as the foundation of numerous pharmaceuticals prescribed for dementia, migraines, hyperprolactinemia, and several other disorders. Information on control of the ergot alkaloid pathway may contribute to strategies to limit their production in agricultural settings or increase their yield for pharmaceutical production. Our results demonstrate that a previously uncharacterized gene clustered with the ergot alkaloid synthesis genes is required for the sufficient transcription of the ergot alkaloid biosynthesis genes. This observation suggests the gene encodes a factor regulating transcription of those biosynthetic genes.

Catalytic Asymmetric Approach to the Naturally Occurring Clavine Alkaloid (+)-Lysergine

AbstractAn efficient asymmetric approach to the ergot alkaloids has been achieved via a highly regioselective Heck cyclization. Asymmetric induction of the key intermediate was achieved via a catalytic enantioselective α-aminoxylation catalyzed by d-proline (98% ee). Utilizing the aforementioned strategy, formal total synthesis of the ergot alkaloids (+)-lysergine and (+)-isolysergine has been achieved. Importantly, an asymmetric approach to the unnatural analogues (–)-lysergine and (–)-isolysergine has also been achieved via catalytic enantioselective α-aminoxylation using l-proline.

Evaluation of Ergot Resistance in Hard Red Spring Wheat in North Dakota.

Higher levels of ergot (Claviceps purpurea [Fr.] Tul.) were reported in North Dakota hard red spring wheat (HRSW) in 2018, leading to questions pertaining to management and cultivar resistance. To better understand pathogen and HRSW cultivar responses, greenhouse experiments were conducted from 2020 to 2021 to evaluate the aggressiveness of nine C. purpurea isolates and ergot resistance in 21 HRSW cultivars. Results from the aggressiveness assay indicated significant cultivar-by-isolate interactions for the total weight of sclerotia produced and ergot incidence. Mean data across all cultivars by isolate combinations suggested isolates CC-3 and IA-Tim were the most aggressive, and these were subsequently used in ergot resistance experiments. Results from ergot resistance screening indicated that none of the HRSW cultivars were immune to C. purpurea because all cultivars produced sclerotia. However, differences in ergot incidence, kernel incidence, aborted kernel incidence, total sclerotia weight, sclerotia length, and sclerotia width occurred among cultivars. Both 'ND-Frohberg' and 'TCG-Spitfire' had the lowest ergot incidence values and were among the lowest in total sclerotia weight. 'Waldron' and 'LCS-Trigger' had the highest ergot incidence and the highest total sclerotia weight. Given that most concerns with ergot occur postharvest, we suggest two categories to describe ergot resistance: host resistance (fate of inoculation for a stigma) and logistical resistance (size characteristics of a sclerotium that influence its ability to remain with a seed lot after harvest and cleaning). This research provides a strong foundation for our understanding of HRSW resistance to ergot that will influence variety decisions in ergot-prone areas in North Dakota.

281 Changes in satellite cell number in maternal and fetal muscle with 5-hydroxy tryptophan supplementation during late gestation

Abstract Toxic tall fescue contains ergot alkaloids, which induce vasoconstriction in livestock, leading to fescue toxicosis. This condition can impact muscle development due to altered blood flow. Supplementation with 5-HTP may counteract the vasoconstrictive effects of fescue alkaloids. We aimed to investigate the influence of 5-HTP supplementation on satellite cells, myoblast, and preadipocyte numbers in maternal and fetal muscle during late gestation in Suffolk ewes. We hypothesized that 5-HTP supplementation would mitigate the effects of fescue toxicosis on muscle cell populations, potentially promoting muscle development and regeneration in maternal and fetal tissues. Suffolk ewes (n = 75), naïve to toxic tall fescue, were blocked by body condition score and body weight into 10 groups for synchronization. Ewes were confirmed pregnant using transabdominal ultrasound, and lamb number was estimated at gestational day (gd) 60. Pregnant ewes carrying twins (n = 30) were randomly assigned to one of three treatments: 1) negative control (CON, n = 10), 2) toxic tall fescue seed containing 1.77 mg ewe-1ּ d-1 ergovaline/ergovalinine plus 5-HTP (2.5 mg/kg BW) supplement (5HTP, n = 10), or 3) positive control, fed toxic tall fescue seed containing 1.77 mg ewe-1ּ d-1 ergovaline/ergovalinine (E+, n = 10). Ewes were individually fed a total mixed ration from gd 78 to gd 85 of pregnancy and then TMR plus treatment (CON, E+ or 5HTP) from gd 86 to d 111. On gd 112, terminal necropsies were performed, and semimembranosus muscle (SM) from the ewe and fetus was removed. Weights were obtained, and then samples were digested using pronase (1.5mg/mL) to isolate preadipocyte (PP1), myoblasts (PP2) and satellite cell (PP3) fractions using a pre-plating technique. Cells were quantified using a Neubauer-hemocytometer and trypan blue to also assess cell viability and changes in cell number. Fetal SM had a greater (P &amp;lt; 0.05) number of pre-adipocytes, myoblasts, and satellite cells per gram of tissue digested in comparison with the ewes. Pre-adipocyte and myoblast number per gram of tissue digested did not differ (P &amp;gt; 0.05) by treatment. The 5HTP group had the greater (P &amp;lt; 0.05) number of satellite cells per gram of tissue digested in comparison with the CON group. Satellite cell numbers tended (P &amp;lt; 0.10) to be greater in the 5HTP group in comparison to the E+ group. Satellite cell numbers from CON and E+ treatment groups did not differ (P &amp;gt; 0.05). In conclusion, 5-HTP supplementation during late gestation in Suffolk ewes exposed to toxic tall fescue significantly increased satellite cell numbers in maternal and fetal muscle tissues. This suggests a potential mitigation of the impact of fescue toxicosis on muscle cell populations.

226 Serotonin stimulates relaxation in ovine saphenous vein precontracted with ergovaline

Abstract The predominant ergot alkaloid found in wild type endophyte-infected tall fescue grass is ergovaline. Ergovaline is a potent vasoconstrictor in livestock that consume it. Previous research has shown that ergovaline can bind serotonin (5-HT) receptors and blood vessels exposed to ergovaline become unresponsive to subsequent 5-HT exposure. Serotonin can stimulate vascular contraction or relaxation depending on the receptor subtype targeted. The objective of this experiment was to evaluate 5HT as vasorelaxant in the ovine saphenous artery and vein that were precontracted with ergovaline. Blood vessels were collected from mixed breed market rams (n = 7) at slaughter, cleaned of surrounding adipose and connective tissue, sliced into 2-mm cross-sections, and mounted in a multimyograph. Artery and vein cross-sections were equilibrated to 1 g tension in continuously gassed (95% O2/5% CO2) Krebs-Henseleit buffer (pH 7.4; 37.5 °C) for 90 min, precontracted with 1.0 μM ergovaline (in a tall fescue seed extract) for 30 min, exposed to increasing concentrations of 5-HT that ranged from 1 x 10-9 to 1 x 10-4 M for a 5-min interval. Response data were normalized to the 1.0 μM ergovaline response and were analyzed as a completely randomized design in SAS. The saphenous vein was larger in diameter blood vessel than the saphenous artery. Both vessel types contracted in response to exposure to 1 μM ergovaline. The saphenous artery had a much lower maximal response to ergovaline than did the vein (P &amp;lt; 0.05) that could be attributed to the size difference. The lateral saphenous vein relaxed to increasing concentrations of 5-HT (P &amp;lt; 0.05) with 87 % relaxation occurring by 1 x 10-6 M 5-HT. Conversely, the lateral saphenous artery contracted in response to increasing concentrations of 5-HT (P &amp;lt; 0.05) resulting in a 43% increase from the ergovaline response by the 1 x 10-4 M addition. This response did not become significant in the artery until 1 x 10-5 M 5-HT compared with 1 x 10-8 M for the vein. This resulted in a 50% effective concentration for 5-HT in the artery of 4.5 compared with 8.1 M for the vein (P &amp;lt; 0.05). The differing responses across vessels are likely a result of different receptor populations and this should be verified in future research. This is the first reported observation of stimulated relaxation of a blood vessel constricted ergovaline. Additional research is needed to understand the mechanism of how 5HT can be used to stimulate vasorelaxation in livestock suffering from ergot alkaloid-induced vasoconstriction.

Bishop–Jones’ theorem and the ergodic limit set

Abstract For a proper, Gromov-hyperbolic metric space and a discrete, non-elementary, group of isometries, we define a natural subset of the limit set at infinity of the group called the ergodic limit set. The name is motivated by the fact that every ergodic measure which is invariant for the geodesic flow on the quotient metric space is concentrated on geodesics with endpoints belonging to the ergodic limit set. We refine the classical Bishop–Jones theorem proving that the packing dimension of the ergodic limit set coincides with the critical exponent of the group.

A Mañé-Manning formula for expanding measures for endomorphisms of ℙ^{𝕜}

Let k ≥ 1 k \ge 1 be an integer and f f a holomorphic endomorphism of P k ( C ) \mathbb {P}^k (\mathbb {C}) of algebraic degree d ≥ 2 d\geq 2 . We introduce a dynamical volume dimension for ergodic f f -invariant probability measures with strictly positive Lyapunov exponents. In particular, this class of measures includes all ergodic measures whose measure-theoretic entropy is strictly larger than ( k − 1 ) log ⁡ d (k-1)\log d , a natural generalization of the class of measures of positive measure-theoretic entropy in dimension 1. The volume dimension is equivalent to the Hausdorff dimension when k = 1 k=1 , but depends on the dynamics of f f to incorporate the absence of an analogue of Koebe’s theorem and the non-conformality of holomorphic endomorphisms for k ≥ 2 k\geq 2 . If ν \nu is an ergodic f f -invariant probability measure with strictly positive Lyapunov exponents, we prove a generalization of the Mañé-Manning formula relating the volume dimension, the measure-theoretic entropy, and the sum of the Lyapunov exponents of ν \nu . As a consequence, we give a characterization of the first zero of a natural pressure function for such expanding measures in terms of their volume dimensions. For hyperbolic maps, such zero also coincides with the volume dimension of the Julia set, and with the exponent of a natural (volume-)conformal measure. This generalizes results by Denker-Urbański [Nonlinearity 4 (1991), pp. 365–384; Trans. Amer. Math. Soc. 328 (1991), pp. 563–587] and McMullen [Comment. Math. Helv. 75 (2000), pp. 535–593] in dimension 1 to any dimension k ≥ 1 k\geq 1 . Our methods mainly rely on a theorem by Berteloot-Dupont-Molino [Ann. Inst. Fourier (Grenoble) 58 (2008), pp. 2137–2168], which gives a precise control on the distortion of inverse branches of endomorphisms along generic inverse orbits with respect to measures with strictly positive Lyapunov exponents.

Variational principle for nonlinear weighted topological pressure

In this paper, we introduce nonlinear weighted topological pressure and establish variational principle for nonlinear weighted topological pressure under the condition that ( π , E ) has an abundance of ergodic measures.

Simultaneous Determination of Alternaria Toxins, Ergot Alkaloid Epimers, and Other Major Mycotoxins in Various Food Matrices by LC‐MS/MS

Simultaneous Determination of Alternaria Toxins, Ergot Alkaloid Epimers, and Other Major Mycotoxins in Various Food Matrices by LC‐MS/MS