Enzyme-induced Carbonate Precipitation Research Articles

Mechanical characterization of calcareous sand reinforced by EICP multivariate tests and synergistic biochar reinforcement

The foundation bearing capacity in island reef infrastructure construction is often inadequate due to the poor engineering properties of calcareous sand. Enzyme-induced carbonate precipitation (EICP) has emerged as an efficient and environmentally friendly method to improve these properties, significantly enhancing the mechanical behavior of calcareous sand. However, the effectiveness of EICP is constrained by environmental factors that affect free urease activity and the limited availability of nucleation sites. To further improve the mechanical properties of EICP-reinforced calcareous sand, this study explores the addition of biochar to the sand. The characteristics of the samples were evaluated using unconfined compressive strength tests, calcium carbonate content measurements, and scanning electron microscopy. The results indicate that incorporating biochar into EICP significantly increases calcium carbonate production, strengthens the reinforced sand, and reduces surface porosity. This improvement is attributed to biochar’s surface properties, which provide additional nucleation sites for calcium carbonate formation, enhancing calcium carbonate content and sand strength. However, controlling the biochar content at approximately 5% is crucial to avoid excessive porosity, which could weaken the material. These findings offer a theoretical foundation for applying EICP-biochar-reinforced calcareous sand in engineering projects.

Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

A comparative study of low-cost crude ureases extracted from multi-species of natural plant sources in enzyme-induced carbonate precipitation

A comparative study of low-cost crude ureases extracted from multi-species of natural plant sources in enzyme-induced carbonate precipitation

Crack Repair in In-Service Tunnel Linings Using Chitosan-Combined Enzyme-Induced Carbonate Precipitation

Crack Repair in In-Service Tunnel Linings Using Chitosan-Combined Enzyme-Induced Carbonate Precipitation

Soil-water retention capacity of expansive soil improved through enzyme induced carbonate precipitation-eggshell powder

Enzyme Induced Carbonate Precipitation (EICP) has been extensively investigated as a promising approach to improve engineering properties of soil, while Eggshell Powder (ESP) is an agricultural waste that effectively fills soil pores. The ESP provides abundant nucleation at sites for the EICP process, further promoting the effective precipitation of calcium carbonate. The research presented in this paper investigated the Soil Water Characteristic Curves (SWCC), permeability coefficient, and microstructure of expansive soil before and after EICP and EICP+ESP modification. A series of laboratory experiments were conducted, including soil water characteristic tests, permeability tests and Scanning Electron Microscopy (SEM). The results proved that the addition of EICP and EICP+ESP into natural expansive soil resulted in a gradual decline in air entry value, residual water content, and permeability coefficient, indicating an increase in water retention capacity and a decrease in permeability. Furthermore, with the intrusion of EICP and EICP+ESP, the contact between particles becomes smoother, and the soil pores become more equally distributed. Ultimately, there was an enhancement in water retention capacity of the natural expansive soil. This study emphasizes the synergistic potential of combining EICP and EICP+ESP as stabilizing additives to enhance the water retention capacity of expansive soil. Moreover, the reuse of ESP provides a sustainable solution for the resource utilization of agricultural waste and the improvement of expansive soil using bio-inspired methods.

Study on the physical properties of red clay cured by different external admixtures combined with enzyme induced carbonate precipitation technology

Study on the physical properties of red clay cured by different external admixtures combined with enzyme induced carbonate precipitation technology

Microscale insights into enzyme-induced carbonate precipitation in rock-based microfluidic chips

Biomineralisation technology shows promise for sealing subsurface fractures, but understanding its microscale mechanisms in real rock fractures remains incomplete. In this study, a microfluidic chip incorporating real rock slices was utilised to conduct enzymatically induced carbonate precipitation (EICP) experiments using a one-phase continuous injection strategy. Real-time observations revealed grey flocs forming and clustering into aggregates susceptible to transport by flow. Calcium carbonate (CaCO3) crystals that formed on rock and polydimethylsiloxane surfaces effectively filled and bridged fractures, leading to a significant pressure drop. Rough surfaces can provide additional sites for solute attachment and heterogeneous nucleation of calcium carbonate. High velocities and small apertures generally promoted floc formation and reduced crystal growth. Precipitation content gradually increased with a decreasing rate. Lower estimated precipitation efficiency was obtained in smaller, high-flow fractures. Precipitates on rock interfaces induced eddies and significant pressure drops within narrow pore throats. Crystal growth displayed a two-stage development: an initial increase followed by a decrease, varying across different facets (the surfaces or orientations of the growing crystals). Growth competition will occur when two or more neighbouring crystals come into contact with diverse grain orientations. The findings provide valuable microscale insights into microscale processes governing EICP within real rock fractures, contributing to evaluation of its efficacy in subsurface fracture sealing applications.

Reducing non-cohesive soil erodibility through enzyme-induced carbonate precipitation

Reducing non-cohesive soil erodibility through enzyme-induced carbonate precipitation

Effect of acid type on biomineralization of soil using crude soybean urease solution

The one-phase-low-pH method is a simple, efficient, and user-friendly biogrouting technique that can effectively improve the biomineralization of enzyme-induced carbonate precipitation (EICP) using free urease enzyme. One of the most significant advantages of this method is its capacity to effectively delay calcium carbonate (CaCO3) precipitation by reducing the pH of the solution through the addition of acid. This prevents bioclogging during the biogrouting process and improves the biomineralization effect. However, the biomineralization of the one-phase-low-pH based EICP method may be influenced by the specific acid used. To investigate the impact of acid type on the one-phase-low-pH EICP method using crude soybean urease solution (CSUS), four types of acids, including hydrochloric acid (HCl), nitric acid (HNO3), acetic acid (CH3COOH), and lactic acid (C3H6O3), were used to adjust the pH of CSUS. A series of macroscopic and microscopic experiments were conducted to evaluate the effect of acid type on the one-phase-low-pH EICP method. The results indicate that the acid has an inhibition on the urease activity (UA) of CSUS. Among the acids tested, HNO3 exhibits the most pronounced inhibitory effect on the UA of CSUS, followed by HCl, and the least pronounced inhibitory effect for CH3COOH and C3H6O3 under the same pH conditions. Meanwhile, CH3COOH and C3H6O3 could provide a longer delay duration of CaCO3 precipitation than HNO3 and HCl. Therefore, the one-phase-low-pH EICP method based on CH3COOH and C3H6O3 can significantly improve the effective biocementation depth compared to that based on HNO3 and HCl. Nevertheless, the different types of acids appear to have no obvious effect on the polymorph and crystalline of the precipitated CaCO3 crystals.

Evaluation of wind erosion resistance of EICP solidified desert sand based on response surface methodology

Wind erosion is the primary cause of land desertification in arid and semi-arid regions. Enzyme-induced carbonate precipitation (EICP) will produce a solid coating on the surface of desert sand that inhibits wind erosion. Three critical variables for EICP treatment, including enzyme-cementation ratio A, cementation solution concentration B, and spraying volume of reaction solution C, were evaluated with three wind erosion-resistance indexes (surface strength, calcium carbonate content, and solidified layer thickness) of desert sand, and further optimized with the response surface methodology (RSM). The mass loss of the EICP-treated desert sand was studied by wind tunnel tests with wind speeds of 8–14 m/s and erosion times of 0–30 min. Material composition and microstructure of the EICP precipitates were investigated with X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Results indicate that the three wind erosion-resistance indexes tend to rise with greater A and B values followed by a decline while monotonically increasing with C but the increments decrease when C>5 L/m2. The optimal solidification effect was reached when A=1.082:1, B=1.282 mol/L and C=8.70 L/m2, which corresponds to a maximum wind speed of 14 m/s for solidified desert sand. Large amounts of calcite crystals were produced in the EICP-treated sand and filled soil pores. The particle contacts transform from point contact to surface contact and the enhanced particle bonding induces a more compact soil skeleton. The above experiment evidence corresponds to increased surface strength and wind erosion resistance. The results of this study will provide an experimental foundation for the application of EICP technology to enhance wind erosion resistance of desert sands.

Assessing the viability of different bio-polymers and synthetic-copolymers with modified enzyme-induced carbonate precipitation solutions for sand consolidation applications

Assessing the viability of different bio-polymers and synthetic-copolymers with modified enzyme-induced carbonate precipitation solutions for sand consolidation applications

Reinforcement of silty sand riverbank using hydroxyethyl cellulose-assisted enzyme-induced carbonate precipitation

Reinforcement of silty sand riverbank using hydroxyethyl cellulose-assisted enzyme-induced carbonate precipitation

Enzyme-Induced Carbonate Precipitation as a Novel Remedy for Expansive Soils: Assessing Microfabric and Swelling Characteristics

Enzyme-Induced Carbonate Precipitation as a Novel Remedy for Expansive Soils: Assessing Microfabric and Swelling Characteristics

Proposing a new sustainable approach for sand improvement using biologically-derived calcium phosphate cement

Bio-mediated soil improvement methods keep on gaining the attention of geotechnical engineers and researchers globally due to their nature-based elegance and eco-friendliness. Most prevalent bio-mediated soil improvement methods include microbial induced carbonate precipitation (MICP) and enzyme induced carbonate precipitation (EICP). During their processes, the bacteria/ free urease hydrolyzes the urea into ammonium and carbonic acid, which is accompanied by a considerable increase of alkalinity (about pH 9.0). The major problem associated with the above techniques is the release of gaseous ammonia that is extremely detrimental. Therefore, this study aims to propose a new sustainable approach involving lactic acid bacteria to facilitate the calcium phosphate mineralization for the strengthening of sand matrix. The major objectives of this investigation are (i) to evaluate the urease activity of the lactic acid bacteria under different temperatures, pH conditions and additions of metal ions, (ii) to assess the treated sand matrix, (iii) to perform cost analysis. The outcomes indicated that Limosilactobacillus sp. could effectively facilitate the urea hydrolysis, hence increase in pH from acidic to neutral, and provided a desirable environment for the calcium phosphate to mineralize within the voids of the sand. The addition of 0.01% Ni2+ in culture media was found to enhance the urease activity by 38.8% and compressive strength over 40%. A combined formation of amorphous- and whisker-like precipitates could bridge a larger area at particle-particle contact points, facilitated a strong force-network in sand matrix. The mineralized calcium phosphate compound was found to be brushite. The cost herein for producing 1L treatment solution was estimated to be about 2.5-folds and 11.8-folds lower compared to that of MICP and EICP treatment solutions, respectively. Moreover, since the treatment pH could potentially be regulated between acidic – neural range, it would greatly control the release of gaseous ammonia. With several environmental and economical benefits, the study has disclosed a new sustainable direction for sand improvement via the use of lactic acid bacteria.

An Enzyme-Induced Carbonate Precipitation Method for Zn2+, Ni2+, and Cr(VI) Remediation: An Experimental and Simulation Study

Heavy metal contamination has long been a tough challenge. Recently, enzyme-induced carbonate precipitation (EICP) has been proposed to handle this problem. This paper aims to explore the efficacy, process, and mechanisms of EICP using crude sword bean urease extracts to remediate Zn2+, Ni2+, and Cr(VI) contamination. A series of liquid batch tests and geochemical simulations, as well as microscopic analyses, were conducted. The liquid batch test results show that Zn2+, Ni2+, and Cr(VI) can be effectively immobilized by the EICP method, and the highest immobilization percentage was observed for Zn2+, reaching up to 99%. Ni2+ and Cr(VI) were immobilized at 62.4% and 24.4%, respectively. Additionally, the immobilization percentage of heavy metals increased with the concentration of added Ca2+. The simulation results and XRD results reveal that the organic molecules in crude sword bean urease can promote ZnCO3, Zn(OH)2, Zn5(CO3)2(OH)6, and NiCO3 precipitation. The FTIR and SEM-EDS results provide evidence for heavy metal adsorption by the functional groups in crude urease and calcium carbonate. The liquid batch test results, as well as the simulation results and the microscopic analysis results, indicate that the mechanism of EICP in heavy metal remediation can be summarized as biomineralization to form heavy metal carbonate precipitates and metal hydroxide precipitates, adsorption by calcium carbonate, and adsorption or complexation or promoting nucleation by organic molecules.

Effect of calcium sources on enzyme-induced carbonate precipitation to solidify desert aeolian sand

Enzyme-induced carbonate precipitation (EICP) is a promising technique for soil reinforcement. To select a suitable calcium source and a suitable solution amount for aeolian sand stabilization using EICP, specimens treated with different solution amounts (1.5, 2, 2.5, 3, and 3.5 L/m2). Surface strength, crust thickness, calcium carbonate content (CCC) and water vapor adsorption tests were performed to evaluate the effect of two calcium sources (calcium acetate and calcium chloride) on aeolian sand solidification. The plant suitability of solidified sand was investigated by the sea buckthorn growth test. The suitable calcium source was then used for the laboratory wind tunnel test and the field test to examine the erosion resistance of solidified sand. The results demonstrated that Ca(CH3COO)2-treated specimens exhibited higher strength than CaCl2-treated specimens at the same EICP solution amount, and the water vapor equilibrium adsorption mass of Ca(CH3COO)2-treated specimens was less, indicating that Ca(CH3COO)2-solidified sand was more effective and had better long-term stability. In addition, plants grown in Ca(CH3COO)2-treated sand had greater seedling emergence percentage and higher average height, which indicated that calcium acetate is a more suitable calcium source for EICP treatment. Furthermore, the surface strength and crust thickness of solidified sand increased with increasing the solution amount. For sand treated with 3 L/m2 of solution, the excessive strength and thickness of the crust made plants growth difficult, and the performance of sand treated with more than 2 L/m2 of solution significantly improved. Thus, the solution amount of 2–3 L/m2 is suggested for engineering applications. The sand solidified using EICP in the field could effectively mitigate wind erosion and facilitate the growth of native plants. Therefore, EICP can be combined with vegetative method to achieve long-term wind erosion control in the future.

Effects of enzyme-induced carbonate precipitation technique on multiple heavy metals immobilization and unconfined compressive strength improvement of contaminated sand

Enzyme-induced carbonate precipitation (EICP) has been studied in remediation of heavy metal contaminated water or soil in recent years. This paper aims to investigate the immobilization mechanism of Zn2+, Ni2+, and Cr(VI) in contaminated sand, as well as strength enhancement of sand specimens by using EICP method with crude sword bean urease extracts. A series of liquid batch tests and artificially contaminated sand remediation experiments were conducted to explore the heavy metal immobilization efficacy and mechanisms. Results showed that the urea hydrolysis completion efficiency decreased as the Ca2+ concentration increased and the heavy metal immobilization percentage increased with the concentration of Ca2+ and treatment cycles in contaminated sand. After four treatment cycles with 0.5 mol/L Ca2+ added, the immobilization percentage of Zn2+, Ni2+, and Cr(VI) were 99.99 %, 86.38 %, and 75.18 %, respectively. The microscale analysis results presented that carbonate precipitates and metallic oxide such as CaCO3, ZnCO3, NiCO3, Zn(OH)2, and CrO(OH) were generated in liquid batch tests and sand remediation experiments. The SEM-EDS and FTIR results also showed that organic molecules and CaCO3 may adsorb or complex heavy metal ions. Thus, the immobilization mechanism of EICP method with crude sword bean urease can be considered as biomineralization, as well as adsorption and complexation by organic matter and calcium carbonate. The unconfined compressive strength of EICP-treated contaminated sand specimens demonstrated a positive correlation with the increased generation of carbonate precipitates, being up to 306 kPa after four treatment cycles with shear failure mode. Crude sword bean urease with 0.5 mol/L Ca2+ added is recommended to immobilize multiple heavy metal ions and enhance soil strength.

Porosity and bedding controls on bio-induced carbonate precipitation and mechanical properties of shale and dolomitic rocks: EICP vs MICP

Biocementation is an emerging field within geotechnical engineering that focuses on harnessing microbiological activity to enhance the mechanical properties and behavior of rocks. It often relies on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP) which utilizes biomineralization by promoting the generation of calcium carbonate (CaCO3) within the pores of geomaterials (rock and soil). However, there is still a lack of knowledge about the effect of porosity and bedding on biocementation in rocks from a mechanistic view. This experimental study investigated the impact of porosity and bedding orientations on the mechanical response of rocks due to biocementations, using two distinct biocementation strategies (MICP and EICP) and characteristically low porosity but interbedded rocks (shale) and more porous but non-bedded (dolostone) rocks. We first conducted biocementation treatments (MICP and EICP) of rock samples over a distinct period and temperature. Subsequently, the rock strength (uniaxial compressive strength, UCS) was measured. Finally, we analyzed the pre- and post-treatment changes in the rock samples to better understand the effect of MICP and EICP biocementations on the mechanical response of the rock samples. The results indicate that biocementations in dolostones can improve the rock mechanical integrity (EICP: +58% UCS; MICP: +25% UCS). In shales, biocementations can either slightly improve (EICP: +1% UCS) or weaken the rock mechanical integrity (MICP: −39% UCS). Further, results suggest that the major controlling mechanisms of biogeomechanical alterations due to MICP and EICP in rocks can be attributed to the inherent porosity, biocementation type, and bedding orientations, and in few cases the mechanisms can be swelling, osmotic suction, or pore pressurization. The findings in this study provide novel insights into the mechanical responses of rocks due to MICP and EICP biocementations.

Feasibility Study of Applying Enzyme-Induced Carbonate Precipitation (EICP) without Calcium Source for Remediation of Lead-Contaminated Loess

Feasibility Study of Applying Enzyme-Induced Carbonate Precipitation (EICP) without Calcium Source for Remediation of Lead-Contaminated Loess

Extraction of high activity bacterial urease and its application to biomineralization of soil

Biomineralization based on bacterial enzyme induced carbonate precipitation (BEICP) process is a promising alternative to cement-based ground treatment technology. The bacterial urease used in BEICP process is usually ultrasonic extracted from urease-producing bacteria. To efficiently extract urease with relatively higher activity from bacterial cells, the ultrasonic extraction parameters of urease were optimized in this study. Next, a series of bacterial urease extraction tests and sand column treatment tests were conducted to investigate the effects of vibration amplitude, upper temperature limit, and cooling method on the urease extraction process and biomineralization of sand. The results show that the upper temperature limit is an important factor affecting the extraction efficiency and the activity of the extracted urease solution, and the optimum upper temperature limit is 50 °C. The results indicate that increasing vibration amplitude could improve the extraction efficiency, but it hardly affects the urease activity (UA) under the optimal temperature. Continuous cooling could effectively simplify the operation and further improve the efficiency of urease extraction. Under the same urease activity of biotreatment solution, there is no marked difference in calcium carbonate content (CCC) and unconfined compressive strength of biomineralized sand columns prepared by urease solution extracted with different vibration amplitudes and upper temperature limits. The results of this study could provide a reference for application of BEICP technology of urease extraction to large-scale soil treatment.