Enzymatic Hydrolysis Of Sugarcane Bagasse Research Articles

Optimizing the Conditions of Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse for Bioethanol Production.

The agricultural waste sugarcane bagasse (SCB) is a kind of plentiful biomass resource. In this study, different pretreatment methods (NaOH, H2SO4, and sodium percarbonate/glycerol) were utilized and compared. Among the three pretreatment methods, NaOH pretreatment was the most optimal method. Response surface methodology (RSM) was utilized to optimize NaOH pretreatment conditions. After optimization by RSM, the solid yield and lignin removal were 54.60 and 82.30% under the treatment of 1% NaOH, a time of 60 min, and a solid-to-liquid ratio of 1:15, respectively. Then, the enzymolysis conditions of cellulase for NaOH-treated SCB were optimized by RSM. Under the optimal enzymatic hydrolysis conditions (an enzyme dose of 18 FPU/g, a time of 64 h, and a solid-to-liquid ratio of 1:30), the actual yield of reducing sugar in the enzyme-treated hydrolysate was 443.52 mg/g SCB with a cellulose conversion rate of 85.33%. A bacterium, namely, Bacillus sp. EtOH, which produced ethanol and Baijiu aroma substances, was isolated from the high-temperature Daqu of Danquan Baijiu in our previous study. At last, when the strain EtOH was cultured for 36 h in a fermentation medium (reducing sugar from cellulase-treated SCB hydrolysate, yeast extract, and peptone), ethanol concentration reached 2.769 g/L (0.353%, v/v). The sugar-to-ethanol and SCB-to-ethanol yields were 13.85 and 11.81% in this study, respectively. In brief, after NaOH pretreatment, 1 g of original SCB produced 0.5460 g of NaOH-treated SCB. Then, after the enzymatic hydrolysis, reducing sugar yield (443.52 mg/g SCB) was obtained. Our study provided a suitable method for bioethanol production from SCB, which achieved efficient resource utilization of agricultural waste SCB.

Enhancing fermentable sugar production from sugarcane bagasse through surfactant-assisted ethylene glycol pretreatment and enzymatic hydrolysis: Reduced temperature and enzyme loading

In this study, an efficient coupling surfactant to ethylene glycol pretreatment (EG) and enzymatic hydrolysis of sugarcane bagasse (SCB) was proposed to enhance fermentable sugar production with lower pretreatment energy consumption and reduced enzyme loading. Under optimized conditions, 5 % Tween 80-assisted EG pretreatment of SCB achieved 80.5 % delignification while retaining cellulose (91.6 %) and hemicellulose (81.6 %) content. This led to an enhanced glucose yield of 81.3 %, compared to 65.1 % without Tween 80. The addition of Tween 80 to pretreatment modified residual lignin through etherification, reduced phenolic hydroxyl groups and enhanced hydrophilicity by 23.7 % and 9.4 %, respectively, compared to the lignin sample without Tween 80. Consequently, this modification alleviated non-productive adsorption between lignin and enzymes, improving substrate hydrolyzability. Moreover, when 4.5 % Triton-X 100 was introduced during the hydrolysis of Tween 80-assisted EG-pretreated substrates, a maximum glucose yield of 91.8 % and xylose yield of 92.6 % were achieved. Energy and enzyme cost analyses revealed a reduction of 35 % in pretreatment energy consumption and a 58.8 % decrease in enzyme costs, thanks to the synergistic action of surfactants in EG pretreatment and enzymatic hydrolysis. Overall, integrating surfactants into pretreatment and enzymatic hydrolysis holds promise for highly efficient conversion of SCB into fermentable sugars in enzyme-mediated lignocellulosic biorefineries.

Quantitative correlation analysis between particle liquefaction and saccharification through dynamic changes of slurry rheological behavior and particle characteristics during high-solid enzymatic hydrolysis of sugarcane bagasse

This study identified the intrinsic relationships among slurry rheology, particle characteristics, and lignocellulosic liquefaction/saccharification based on correlation analysis and principal component analysis during the hydrolysis of sugarcane bagasse pretreated by deep eutectic solvents (DES) and mechanical milling (MM). The DES-MM pretreated lignocellulosic slurry (20% solids) exhibited high apparent viscosity of 1.4 × 104 Pa·s and shear stress of 929.0 Pa under steady state. Glucose production had a negative linear correlation with slurry viscosity (R2, 0.69–0.97), whereas its correlation with yield stress (R2, 0.85–0.98) depended on the particle liquefaction rate. The availability of free water provided a major contribution to improving slurry rheology. However, the size reduction of submillimeter particles and the changes in particle hydrophilicity during liquefaction were not significantly correlated with rheological changes. Various interrelated particle characteristics and rheological changes were integrated into two simple principal variables to predict glucose production with a high R2 of 0.96.

Comparative study for enzymatic hydrolysis of sugarcane bagasse using free and nanoparticle immobilized holocellulolytic enzyme cocktail

This study aimed at a comparative analysis of enzymatic hydrolysis of sugarcane bagasse biomass by using a free holocellulase cocktail of an enzyme (Cellic CTec2) and immobilized enzyme system on IOMNP@SiO2-NH2 support to make the process cost-effective. The immobilization was substantiated by examining the nano biocatalysts using FTIR, TEM, and DLS analysis. IOMNP@SiO2 –NH2 and immobilized enzyme system have average particle sizes of 81.1, and 455.9 nm confirmed by DLS. Response surface methodology (RSM) based on the Box Behnken design was employed to optimize the saccharification process conditions. The free cellulase cocktail showed a maximum hydrolysis yield of 70.71 % at 12.5FPU/gm enzyme dose, while the immobilized enzyme at a dose of 20 FPU/gm achieved a conversion rate of 74.19 % under optimized parameters likes hydrolysis time, surfactant and substrate concentration. Pretreated sugarcane bagasse substrate was used to evaluate the reusability potential of magnetically immobilized cellulase enzyme compared to recovered free enzyme up to 5 cycles. In the second and third cycles, the conversion rates of cellulose to glucose were determined to be 68.21 %, and 52.6 %, respectively. The findings of this study demonstrate that the immobilized Cellic CTec2 enzyme cocktail on functionalized IOMNP is effective for hydrolyzing sugarcane bagasse which also provides the advantages of easy recovery and reusability of enzyme for multiple hydrolysis cycles.

A Novel Kinetic Modeling of Enzymatic Hydrolysis of Sugarcane Bagasse Pretreated by Hydrothermal and Organosolv Processes.

Lignocellulosic biomasses have a complex and compact structure, requiring physical and/or chemical pretreatments to produce glucose before hydrolysis. Mathematical modeling of enzymatic hydrolysis highlights the interactions between cellulases and cellulose, evaluating the factors contributing to reactor scale-up and conversion rates. Furthermore, this study evaluated the influence of two pretreatments (hydrothermal and organosolv) on the kinetics of enzymatic hydrolysis of sugarcane bagasse. The kinetic parameters of the model were estimated using the Pikaia genetic algorithm with data from the experimental profiles of cellulose, cellobiose, glucose, and xylose. The model considered the phenomenon of non-productive adsorption of cellulase on lignin and inhibition of cellulase by xylose. Moreover, it included the behavior of cellulase adsorption on the substrate throughout hydrolysis and kinetic equations for obtaining xylose from xylanase-catalyzed hydrolysis of xylan. The model for both pretreatments was experimentally validated with bagasse concentration at 10% w/v. The Plackett-Burman design identified 17 kinetic parameters as significant in the behavior of process variables. In this way, the modeling and parameter estimation methodology obtained a good fit from the experimental data and a more comprehensive model.

Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80

Delignification pretreatment with alkali under various conditions (25–160 °C for 1–12 h) or sodium chlorite at 75 °C for 4 h was applied to improve the enzymatic digestibility of sugarcane bagasse by removing hemicellulose and lignin. Compared with the elimination of hemicellulose, delignification contributed more in achieving a higher glucose yield. In addition, the characterization of untreated and pretreated sugarcane bagasse was conducted to determine the influence of hemicellulose and lignin degradation on subsequent enzymatic digestibility. Furthermore, Polysorbate 80 was added to reduce the enzyme loading, shorten the hydrolysis time, and enhance the efficiency of enzymatic hydrolysis, suggesting that the glucose yield of 92.2% was obtained with enzyme loading of 5 FPU/g substrate. However, the increased yield of glucose with Polysorbate 80 occurred with an increased lignin content and a reduction of enzyme loading, and the yield decreased sharply as the hydrolysis time was prolonged from 6 h to 24 h.

Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action

This study evaluated innovative approaches for the enzymatic hydrolysis of lignocellulosic biomass. More specifically, assays were performed to evaluate the supplementation of the commercial cellulolytic cocktail Cellic® CTec2 (CC2) with LPMO (GcLPMO9B), H2O2, or cello-oligosaccharide dehydrogenase (CelDH) FgCelDH7C in order to boost the LPMO action and improve the saccharification efficiency of biomass into monosaccharides. The enzymatic hydrolysis was carried out using sugarcane bagasse pretreated by hydrodynamic cavitation-assisted oxidative process, 10% (w/w) solid loading, and 30 FPU CC2/g dry biomass. The results were compared in terms of sugars release and revealed an important influence of the supplementations at the initial 6 h of hydrolysis. While the addition of CelDH led to a steady increase in glucose production to reach 101.1 mg of glucose/g DM, accounting for the highest value achieved after 72 h of hydrolysis, boosting the LPMOs activity by the supplementation of pure LPMOs or the LPMO co-substrate H2O2 were also effective to improve the cellulose conversion, increasing the initial reaction rate of the hydrolysis. These results revealed that LPMOs play an important role on enzymatic hydrolysis of cellulose and boosting their action can help to improve the reaction rate and increase the hydrolysis yield. LPMOs-CelDH oxidative pairs represent a novel potent combination for use in the enzymatic hydrolysis of lignocellulose biomass. Finally, the strategies presented in this study are promising approaches for application in lignocellulosic biorefineries, especially using sugarcane bagasse as a feedstock.

Wastes recycling of non-sterile cellulosic ethanol production from low-temperature pilot-scale enzymatic saccharification of alkali-treated sugarcane bagasse

High energy consumption has been one of bottlenecks for bioconversion of lignocellulose into biofuels and biochemicals. A self-designed pilot-scale device with recycling of black liquor (BL) and waste washing water (WWW) was constructed to conduct low-temperature pretreatment and high-solid enzymatic hydrolysis of sugarcane bagasse (SCB) at ordinary atmospheric pressure. Results showed that enzymatic hydrolysis of WWW-washed BL-WWW-NaOH-treated SCB at 30% solid concentration for 72 h achieved 91.59 g/L glucose with glucan conversion of 70.94%. The pretreatment unit shared only 19.68%–22.43% of total energy consumption and 8.32%–8.81% of total cost. The non-sterile enzymatic hydrolysate could be directly fermented by BL-adapted yeasts to maximally produce 44.82 g/L ethanol at 48 h, while the sterile hydrolysate could not be fermented. 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, furancarboxaldehyde and 5-hydroxymethylfurfural might be the dominant inhibitors in the sterile enzymatic hydrolysate for ethanol production. Finally, a low-emission strategy for cellulosic ethanol production with low energy consumption was proposed.

Ultrasonic-Assisted Dual-Alkali Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse Followed by Candida tropicalis Fermentation to Produce Xylitol.

In this work, the investigation mainly focused on ultrasonic-assisted dual-alkali pretreatment and enzymatic hydrolysis of sugarcane bagasse followed by Candida tropicalis fermentation to produce xylitol. The results showed that the combination of NaOH and ammonia water had the best effect by comparing the effects of the four single-alkali (NaOH, KOH, ammonia water, Ca(OH)2) and their mixed double-alkali pretreatments on xylose content. Then, the optimal conditions for ultrasonic-assisted pretreatment and enzymatic hydrolysis of sugarcane bagasse were obtained by response surface methodology. When the ratio of NaOH and ammonia water was 2:1, the mixed alkali concentration (v/v) was 17%, the ultrasonic temperature was 45°C, the ultrasonic power was 300 W, and the ultrasonic time was 40 min, the content of xylose reached a maximum of 2.431 g/L. Scanning electron microscopy showed that sugarcane bagasse by ultrasonic-assisted alkali pretreatment aggravated with more folds and furrows. Moreover, the fermentation results showed that the concentration ratio of enzymatic hydrolysate of sugarcane bagasse affected the xylitol yield, and when concentrated three times, the highest yield of xylitol (54.42%) was obtained.

When the order matters: Impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse

Dilute acid and alkaline pretreatments are commonly used to fractionate lignocellulosic biomass for its conversion into biofuels and renewable materials. In the current work, we compare the structure and morphology of one and two-step alkaline and acid pretreated sugarcane bagasse (SCB) and differences in their enzymatic hydrolysis rates and yields. We used physical techniques such as nuclear magnetic resonance (NMR) and X-ray diffraction to shed light on physical and morphological changes introduced by the pretreatments in SCB. We also applied solid state NMR procedures which allowed us to separate xylan structural conformations inside pretreated plant biomass samples. Our results reveal that practically all xylan in two-step “alkaline first” (NaOH 1% + H2SO4 1%) pretreated samples adopts two-fold ribbon-like conformation, tightly associated with crystalline cellulose. On the contrary, a main part of xylan in the SCB samples after “acid first” (H2SO4 1% + NaOH 1%) combined pretreatment has a three-fold screw conformation characteristic of xylan bound to lignin. Modifications in the biomass composition, physical structure and xylan conformation introduced by the alternative pretreatments contribute to the observed differences in the enzymatic hydrolysis rates and yields. These results could be relevant for analysis and optimization of pretreatments and enzymatic hydrolysis of lignocellulosic biomass in general.

Alkaline hydrogen peroxide pretreatment combined with bio-additives to boost high-solids enzymatic hydrolysis of sugarcane bagasse for succinic acid processing

Alkaline hydrogen peroxide (AHP) pretreatment of sugarcane bagasse (SCB) at mild conditions was optimized with response surface methodology (RSM), then enzymatic hydrolysis was performed at high-solids substrate loading (30 %, w/v), followed by fed-batch fermentation to convert the fermentable sugars into succinic acid (SA). Results showed the AHP pretreatment conditions of H2O2 concentration 5.5 % (v/v), solid-to-liquid ratio 0.08, pretreatment temperature 65 °C and time 5 h could achieve the highest sugar yield (74.3 %); both additives and fed-batch strategy were favored to boost enzymatic hydrolysis, the concentration and yield of total sugars reached to 195 g/L and 70 % with cellulase dosage of only 6 FPU/g dry biomass (DM); all glucose and xylose could be utilized after fed-batch fermentation, and the obtained concentration and yield of SA reached 41.4 g/L and 63.8 %. In summary, a SA conversion rate high to 0.29 g/g SCB raw material could be achieved via the developed process.

High-solid enzymatic hydrolysis of sugarcane bagasse and ethanol production in repeated batch process using column reactors

The online version contains supplementary material available at 10.1007/s13205-021-02932-3.

Characteristics of sugarcane bagasse fibers after xylan extraction and their high-solid hydrolysis cellulase-catalyzed

The enzymatic hydrolysis of sugarcane bagasse to obtain a high efficiency conversion of polysaccharides in monosaccharides is dependent on factors related to the morphology and chemical composition. Therefore, a pre-treatment step is necessary to obtain a less recalcitrant substrate. Here, alkaline sulfite with ethanol (ASE) was employed to pretreat sugarcane bagasse aiming to produce substrates enriched in carbohydrates with greater cellulose accessibility. A xylanase stage was evaluated as a strategy to remove arabinoxylan fraction from pretreated bagasse and to verify the influence of the addition of the enzyme xylanase (5, 50, and 500 IU/g material) on the characteristics of ASE pretreated bagasse from 5 to 15% dry matter. The highest xylan extraction yield of 59% was achieved when working at 15% solids and xylanase dosage of 500 IU/g substrate. The interaction of enzyme and solid loadings was positive to arabinoxylan extraction, however the excessive removal of arabinoxylans caused changes on biomass fiber surface and a decrease in water absorption. The cellulase-catalyzed hydrolysis of ASE bagasse ASE pre-extracted by xylanase was nearly paralleled to the control sample, at 15% solids. This study shows that the enzymatic removal of xylan from pretreated bagasse provides a promising condition for the generation of the value-added product in the biorefinery process.

Intensification of sugar production by using Tween 80 to enhance metal-salt catalyzed pretreatment and enzymatic hydrolysis of sugarcane bagasse

In this study, different metal-salt catalyzed pretreatment was presented to disorganize the obstinate structure by eliminating the majority of hemicellulose, fractional of lignin, and improve the enzymatic saccharification of sugarcane bagasse. With the accession of Tween 80 during enzymolysis, all metal-salt pretreated substrates presented higher glucose yields, especially for CuCl2. Furthermore, Tween 80 was added to the pretreatment, enhancing the elimination of hemicellulose and lignin, decreasing the degradation of sugars to inhibitors, and presenting superior performance on improving glucose yield. In addition, the maximum glucose yield of 88.0% was achieved by using Tween 80 concomitantly with AlCl3 pretreatment and enzymolysis. It was also found that adding Tween 80 during pretreatment or/and enzymolysis after 24 h could liberate the similar glucose without Tween 80 after 72 h. However, the enhancement of Tween 80 at 6 h was higher than that at 72 h.

The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse

The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse

Multi-efficient thermostable endoxylanase from Bacillus velezensis AG20 and its production of xylooligosaccharides as efficient prebiotics with anticancer activity

In this study, we have unveiled the novel potential of xylanase production by Bacillus velezensis, previously known only for its PGPR traits. According to our investigations, Bacillus velezensis AG20 produced extracellular xylanase with a fold purification of 5.3 and a yield of 21 %. The purified xylanase (45 kDa) aptly cleaved linear β-(1→4)-xylan with a maximum velocity of 21.0 ± 3.0 U/mL, a turnover of 1.75/s, and a catalytic cycle of 0.571 at optimum pH 7. The temperature of 50 °C was found optimum for enzyme activity. Purified endoxylanase was thermostable and retained its significant residual activities at 50 °C and 60 °C. Metal cations such as Ca2+ and Mg2+ enhanced the substrate catalysis up to 1.5 fold. The purified endoxylanase also demonstrated multi-substrate hydrolyzing properties. However, the enzymatic hydrolysis of sugarcane bagasse produced xylooligosaccharides (XOS), including xylobiose, xylotriose, and xylotetrose. The mixture of released XOS has profound stability in gastric juice, intestinal fluid, and α-amylase and facilitates probiotic bacteria Bifidobacterium. The mixed XOS (300 μg/mL) significantly inhibited the HT-29 and Caco-2 cell proliferation by 90 % and 75 %, respectively, at 48 h. The possible mechanism of XOS uptake through the BIAXP receptor of Bifidobacterium has been contemplated and established using the caver analysis, Molecular Dynamics (MD) simulation, and Density Functional Theory (DFT) studies. Thus, here we reveal the utility of this novel bacterial strain in industries as high-temperature catalysts.

Co-production of fermentable glucose, xylose equivalents, and HBS-lignin from sugarcane bagasse through a FeCl3-catalyzed EG/H2O pretreatment

In this study, a FeCl3-catalyzed ethylene glycol/water (FeCl3-EG/H2O) pretreatment was developed and applied for the enzymatic hydrolysis of sugarcane bagasse. The results indicated that FeCl3-EG/H2O pretreatment showed excellent selectivity for the removal of both hemicellulose (∼100 %) and lignin (∼65.3 %), leading to the residual pretreated solid with high cellulose content (∼74.7 %) that could be easily for subsequent enzymatic saccharification. The yield of glucose after enzymatic hydrolysis was almost linearly positively correlated with the removal of lignin and xylan, and the maximum glucose yield obtained the using method was 91.5 %, which was 4.1-fold as large as that without pretreatment. The SEM, FT-IR, and XRD analysis indicated that the improvement of enzyme digestibility of sugarcane bagasse was not only related to the selectively remove of some component barriers, but was also correlated with the changes of surface morphology and internal structure. Furthermore, part of the hemicellulose and lignin in sugarcane bagasse also can be recovered in xylose equivalents, furfural, and high boiling solvent lignin (HBS-lignin) with the yields of 84.0 %, 12.5 %, and 60.1 %, respectively. It can be concluded that the FeCl3-EG/H2O pretreatment developed in this study was effective in achieving the hierarchic conversion and utilization of bagasse.

Impact of Alkaline Pretreatment Condition on Enzymatic Hydrolysis of Sugarcane Bagasse and Pretreatment Cost.

A combined severity factor (RCSF) which is usually used to evaluate the effectiveness of hydrothermal pretreatment at above 100 °C had been developed to assess the influence of temperature, time, and alkali loading on pretreatment and enzymatic hydrolysis of lignocellulose. It is not suitable for evaluating alkaline pretreatment effectiveness at lower than 100 °C. According to the reported deducing process, this study modified the expression of [Formula: see text] as [Formula: see text] which is easier and more reasonable to assess the effectiveness of alkaline pretreatment. It showed that RCSF exhibited linear trend with lignin removal, and quadratic curve relation with enzymatic hydrolysis efficiency (EHE) at the same temperature. The EHE of alkali-treated SCB could attain the maximum value at lower RCSF, which indicated that it was not necessary to continuously enhance strength of alkaline pretreatment for improving EHE. Within a certain temperature range, the alkali loading was more important than temperature and time to influence pretreatment effectiveness and EHE. Furthermore, the contribution of temperature, time, and alkali loading to pretreatment cost which was seldom concerned was investigated in this work. The alkali loading contributed more than 70% to the pretreatment cost. This study laid the foundation of further optimizing alkaline pretreatment to reduce cost for its practical application.

Sequential fed batch extractive fermentation for enhanced bioethanol production using recycled Spathaspora passalidarum and mixed sugar composition

The simultaneous ethanol production and removal during sequential cell recycle fed batch fermentation provides a complementary route to produce this biofuel from sugar mixtures, which may greatly improve yields and productivity from lignocellulosic hydrolysates. Spathaspora passalidarum is a wild-type strain able to naturally convert glucose, fructose, xylose and arabinose into ethanol. Therefore, the present work has focused on 2G bioethanol production by S. passalidarum aiming at the consumption of all sugars released after pre-treatment and enzymatic hydrolysis of sugarcane bagasse in a single fermentation step. The fermentation strategy with sequential cell recycle, fed-batch mode and ethanol removal in situ was performed on a hemicellulosic hydrolysate medium supplemented with molasses. This strategy gave improved fermentation performance and enabled the co-fermention of all sugars under microaerobic conditions. The maximum ethanol yield and productivity was 0.482 g.g−1 and 9.5 g·L-1·h−1, respectively, showing a process efficiency of 94.3%. The selective ethanol removal enables the operation of the bioreactor at low levels of ethanol (20–30 g·L-1), even with high sugar concentration inputs, accelerating the fermentation performance and avoiding inhibitory effects on yeast metabolism. Applying the cell recycle strategy, S. passalidarum was able to increase its robustness, as shown by a 10-fold increase in ethanol productivity, and it was also able to tolerate a high acetic acid concentration (4.5 g·L-1) during long-term fermentations. These results demonstrate that the bioprocess strategy has a strong potential to improve bioethanol production of rich mixed sugar from lignocellulosic hydrolysates in a single fermentation step.

Production of lignocellulolytic enzymatic complex using pretreated carnauba straw as carbon source and application on sugarcane bagasse hydrolysis

Lignocellulolytic enzymes have a great biotechnological potential. Their commercial exploitation has been considerably increased in recent years. However, its industrial application in the conversion of lignocellulosic biomass to biofuels depends on the development of more economical processes and technologies for the lignocellulolytic enzyme production. In this study, the improvement of cellulases and xylanases by Trichoderma reesei CCT-2768 production induced by pretreated carnauba straw with alkaline hydrogen peroxide (A-HP) was performed and its crude extract produced was applied in the enzymatic hydrolysis of sugarcane bagasse. Herein, three conditions were carried out consisting of changing solid loading, pretreatment time, and hydrogen peroxide concentration. The FPase, CMCase, xylanase, and β-glucosidase activities of produced crude extracts were measured. Pretreated carnauba straw had a higher production of total cellulase (2.4 U/g dry substrate) and xylanase (172 U/g dry substrate) enzymes. The low cellulases (8.0 U/g dry substrate) and high xylanase charges (544 U/g dry substrate) present in the produced extract and applied into pretreated sugarcane bagasse hydrolysis allowed a hydrolysis efficiency of 86.96%. The in situ lignocellulolytic enzyme production can represent a relevant advance in the future in overall cost reducing enzymatic hydrolysis process of lignocellulosic materials.