Waste tempura flakes collected from local restaurants were upcycled to be utilized as a polymer filler material. The oil fraction in collected waste tempura flakes were extracted two times via centrifugal solid-liquid separation and organic solvent extraction. Then, oil-extracted residual starch was freeze-milled to produce fine powders. Waste tempura flake-derived starch powder was substituted with 1%, 3%, and 5% (wt%) of virgin starch powder to produce thermoplastic starch. Composition, X-ray diffraction, and Fourier transform infrared analyses showed that the mixture was successfully thermally plasticized. Substitution of waste tempura flake-derived starch decreased tensile strength while increasing elongation at break of some samples. Produced thermoplastic starch samples again were compounded with polylactic acid (PLA) and poly-butylene adipate-co-terephthalate (PBAT) following a mixing ratio of 3:5:2. The analyses indicated that thermoplastic starch, PLA, and PBAT were successfully compounded. The compound containing 1 wt% substituted thermoplastic starch showed the minimal mechanical strength decrease. This study revealed the possibility of upcycling waste fried food into a valuable bioplastic material. Waste tempura flakes can be utilized as both bio-jet fuel and bioplastic filler material.