This paper uses seven periods of land use/cover data from 1990 to 2020 to investigate the spatio-temporal features of land use change in a typical transect in northern China. By using the land use transfer matrix, transect analysis, and ecological contribution rate, image interpretation data obtained from the Chinese Academy of Sciences Resource and Environmental Science Data Platform were analyzed using a spatial overlay to quantitatively examine the transect’s land use changes and their impact on the eco-environment. The results indicate that (1) the transect’s land use is dominated by cropland and grassland. (2) Cropland, forest land, and waters experienced significant transitions in 1995 and 2000, which was linked to socio-economic development and policy factors. (3) The total dynamic percentage of land use change is 4.52%, with built-up land and cropland showing the highest change. (4) The transect’s eco-environmental quality (EQ) has significantly declined, with the quality index dropping from 0.3839 to 0.3773. The transformation of cropland to forest land improves the eco-environment, while the transformation to built-up land has negative impacts. Human activities adversely affect the EQ. The findings are promising for leading the development of conserving the eco-environment and supporting the formation of regionally differentiated paths of ecological civilization construction in the transect.
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