Entry Gate Research Articles

Mud Hive: An Eco-friendly Approach for Apis mellifera

The stationary mud hive, designed as a non-transportable alternative to the BIS wooden hives, adheres to the same frame dimensions. A site with direct sunlight is chosen, and the hive is built using a mud mixture, iron planks, and standard Indian bricks (7.5 × 3.54 × 3.54 inches). A brick with a bee entry gate (6 × 2 inches) is made. The entire structure is then layered with mud, providing effective thermal insulation. After drying and sintering for strength, it holds up to 10 BIS frames. This traditional construction approach offers a reliable and environmentally sustainable alternative to modern beekeeping methods.

Dynamic basis of lipopolysaccharide export by LptB2FGC

Lipopolysaccharides (LPS) confer resistance against harsh conditions, including antibiotics, in Gram-negative bacteria. The lipopolysaccharide transport (Lpt) complex, consisting of seven proteins (A-G), exports LPS across the cellular envelope. LptB2FG forms an ATP-binding cassette transporter that transfers LPS to LptC. How LptB2FG couples ATP binding and hydrolysis with LPS transport to LptC remains unclear. We observed the conformational heterogeneity of LptB2FG and LptB2FGC in micelles and/or proteoliposomes using pulsed dipolar electron spin resonance spectroscopy. Additionally, we monitored LPS binding and release using laser-induced liquid bead ion desorption mass spectrometry. The β-jellyroll domain of LptF stably interacts with the LptG and LptC β-jellyrolls in both the apo and vanadate-trapped states. ATP binding at the cytoplasmic side is allosterically coupled to the selective opening of the periplasmic LptF β-jellyroll domain. In LptB2FG, ATP binding closes the nucleotide binding domains, causing a collapse of the first lateral gate as observed in structures. However, the second lateral gate, which forms the putative entry site for LPS, exhibits a heterogeneous conformation. LptC binding limits the flexibility of this gate to two conformations, likely representing the helix of LptC as either released from or inserted into the transmembrane domains. Our results reveal the regulation of the LPS entry gate through the dynamic behavior of the LptC transmembrane helix, while its β-jellyroll domain is anchored in the periplasm. This, combined with long-range ATP-dependent allosteric gating of the LptF β-jellyroll domain, may ensure efficient and unidirectional transport of LPS across the periplasm.

Dynamic basis of lipopolysaccharide export by LptB2FGC.

Lipopolysaccharides (LPS) confer resistance against harsh conditions, including antibiotics, in Gram-negative bacteria. The lipopolysaccharide transport (Lpt) complex, consisting of seven proteins (A-G), exports LPS across the cellular envelope. LptB2FG forms an ATP-binding cassette transporter that transfers LPS to LptC. How LptB2FG couples ATP binding and hydrolysis with LPS transport to LptC remains unclear. We observed the conformational heterogeneity of LptB2FG and LptB2FGC in micelles and/or proteoliposomes using pulsed dipolar electron spin resonance spectroscopy. Additionally, we monitored LPS binding and release using laser-induced liquid bead ion desorption mass spectrometry. The β-jellyroll domain of LptF stably interacts with the LptG and LptC β-jellyrolls in both the apo and vanadate-trapped states. ATP binding at the cytoplasmic side is allosterically coupled to the selective opening of the periplasmic LptF β-jellyroll domain. In LptB2FG, ATP binding closes the nucleotide binding domains, causing a collapse of the first lateral gate as observed in structures. However, the second lateral gate, which forms the putative entry site for LPS, exhibits a heterogeneous conformation. LptC binding limits the flexibility of this gate to two conformations, likely representing the helix of LptC as either released from or inserted into the transmembrane domains. Our results reveal the regulation of the LPS entry gate through the dynamic behavior of the LptC transmembrane helix, while its β-jellyroll domain is anchored in the periplasm. This, combined with long-range ATP-dependent allosteric gating of the LptF β-jellyroll domain, may ensure efficient and unidirectional transport of LPS across the periplasm.

Identifying strategies to enhance the milking and operator efficiency of herringbone and rotary parlor systems in Ireland

International trends of increasing dairy herd sizes coupled with scarcity of labor has necessitated the enhancement of labor efficiency for dairy production systems. This study quantified the effects of infrastructure, automation, and management practices on the milking and operator efficiency of herringbone and rotary parlors used on pasture-based farms in Ireland. Data from 592 milkings across 26 farms (16 herringbones and 10 rotaries) was used. The metrics of cows milked per hour (cows/h), cows milked per operator per hour (cows/h per operator) and liters of milk harvested per hour (L/h) described milking efficiency. The metrics of total process time per cow (TPT, s/cow), milk process time per cow (MPT, s/cow), work routine time (WRT, s/cow), cluster time (CT, s/cluster), and attachment time per cow (ATC, s/cow) described operator efficiency. Automations investigated were backing gates, cluster flush, plant wash, cluster removers (ACRs), feeders, entry gates, rapid-exit, and teat spray. Additional operator presence at milking was also investigated. Herringbone and rotary parlors were assigned to quartiles from their cows/h per operator values to examine infrastructure, automations, and management practices variations. Fourth quartile herringbones based on cows/h per operator values (Q4) averaged 93 cows/h per operator using average system sizes of 24 clusters with 5 parlor automations. Q4 rotaries averaged 164 cows/h per operator using average system sizes of 47 clusters and an average CT of 13 s/cluster. Cows/h per operator values for Q4 herringbone and rotary parlors were 82% and 54% higher, respectively, than values observed on Q1 parlors, indicating the considerable potential to improve efficiency. To determine if infrastructure, automations, or additional operators at milking significantly affected operator efficiencies, general linear mixed models were developed. For parlor infrastructure, additional clusters had greater significance on operator efficiencies (MPT) for herringbones (-1.3 s/cow) as opposed to rotaries (-0.2 s/cow). Hence, increases in system size was likely to result in improved efficiencies for herringbones but less so for rotaries. For automations, ACRs significantly reduced herringbone TPT, CT, and WRT values by 13.3 s/cow, 18.9 s/cluster, and 32.6 s/cow, respectively, whereas rapid-exit significantly lowered CT by 18.6 s/cluster. We found no significant effect on rotary TPT, MPT, CT, or WRT values from the use of automatic teat sprayers. An additional operator at milking was found to significantly reduce herringbone TPT but not MPT or CT. For rotaries, a second operator had no significant effect on TPT, MPT, CT, or WRT values. We documented strong negative correlations between operator efficiencies (TPT, MPT) and milking efficiency (cows/h) for both herringbone (-0.91, -0.84) and rotaries (-0.98, -0.89). Strong negative correlations between the herringbone automation count and TPT (-0.80), MPT (-0.72), and CT (-0.75) suggested highly automated parlors were likely to achieve greater operator efficiencies than less automated parlors. The strong negative correlation (-0.81) between rotary milking efficiency (cows/h) and CT suggested lower CT values (i.e., rotation speed) resulted in increased milking efficiency. In conclusion, our study quantified the effects of parlor infrastructure, automation, and management practices on the milking and operator efficiency of herringbone and rotary parlors.

DYRK1A signalling synchronizes the mitochondrial import pathways for metabolic rewiring

Mitochondria require an extensive proteome to maintain a variety of metabolic reactions, and changes in cellular demand depend on rapid adaptation of the mitochondrial protein composition. The TOM complex, the organellar entry gate for mitochondrial precursors in the outer membrane, is a target for cytosolic kinases to modulate protein influx. DYRK1A phosphorylation of the carrier import receptor TOM70 at Ser91 enables its efficient docking and thus transfer of precursor proteins to the TOM complex. Here, we probe TOM70 phosphorylation in molecular detail and find that TOM70 is not a CK2 target nor import receptor for MIC19 as previously suggested. Instead, we identify TOM20 as a MIC19 import receptor and show off-target inhibition of the DYRK1A-TOM70 axis with the clinically used CK2 inhibitor CX4945 which activates TOM20-dependent import pathways. Taken together, modulation of DYRK1A signalling adapts the central mitochondrial protein entry gate via synchronization of TOM70- and TOM20-dependent import pathways for metabolic rewiring. Thus, DYRK1A emerges as a cytosolic surveillance kinase to regulate and fine-tune mitochondrial protein biogenesis.

Architectural interventions to mitigate the spread of SARS-CoV-2 in emergency departments

Emergency departments (EDs) are a critical entry gate for infectious agents into hospitals. In this interdisciplinary study, we explore how infection prevention and control (IPC) architectural interventions mitigate the spread of emerging respiratory pathogens using the example of SARS-CoV-2 in a prototypical ED. Using an agent-based approach, we integrated data on patients' and healthcare workers' (HCWs) routines and the architectural characteristics of key ED areas. We estimated the number of transmissions in the ED by modelling the interactions between and among patients and HCWs. Architectural interventions were guided towards the gradual separation of pathogen carriers, compliance with a minimum interpersonal distance, and deconcentrating airborne pathogens (higher air exchange rates (AERs)). Interventions were epidemiologically evaluated for their mitigation effects on diverse endpoints. Simulation results indicated that higher AERs in the ED (compared with baseline) may provide a moderate level of infection mitigation (incidence rate ratio (IRR) of 0.95 (95% confidence interval (CI) 0.93-0.98)) while the overall burden decreased more when rooms in examination areas were separated (IRR of 0.78 (95% CI 0.76-0.81)) or when the size of the ED base was increased (IRR of 0.79 (95% CI 0.78-0.81)). The reduction in SARS-CoV-2-associated nosocomial transmissions was largest when architectural interventions were combined (IRR of 0.61 (95% CI 0.59-0.63)). These modelling results highlight the importance of IPC architectural interventions; they can be devised independently of profound knowledge of an emerging pathogen, focusing on technical, constructive, and functional components. These results may inform public health decision-makers and hospital architects on how IPC architectural interventions can be optimally used in healthcare premises.

Offline Entry and Exit Gate Management System: A Barcode-Based Registration System at Jigme Namgyel Engineering College

The barcode system is used to automate data collection and is valuable to eliminate the possibility of human error, and the data obtained through the barcode is generated rapidly. Further, barcode scanning automatically enters a large amount of data into a system, making it efficient for recordkeeping and streamlining the records. Previously, Jigme Namgyel Engineering College (JNEC), had a paper-based registration system. It generated a great deal of paper, and time was consumed in long queues while students entered and exited the campus. Therefore, a QR system was developed whereby users scan outgoing/incoming QR through Google scanners and fill an online form. The system was also found to be time-consuming as the user had to punch in details manually and there was also a need for an internet connection. Although the system did help curb paper wastage. Therefore, to overcome the limitations of both systems, a Barcode System has been developed, wherein the barcode present in the user ID card is scanned and the user details will be auto-generated in the system. This system was developed and designed using Microsoft Access (MS Access). Problem-Based Learning (PBL) methodology was also implemented along with the System Development Life Cycle (SDLC). The system allows users to view and print the user record based on department. Generate users’ entry and exit reports, and also sort the report by date. The Barcode system is found to be the quickest and easiest way for the user to get registered. It is essential because it fulfills the primary role of keeping the record of outgoing or incoming users faster and easier. The Barcode system also fulfills the aim of going green by reducing paper wastage.

Unveiling a novel entry gate: Insect foregut as an alternative infection route for fungal entomopathogens

Insects and their natural microbial pathogens are intertwined in constant arms races, with pathogens continually seeking entry into susceptible hosts through distinct routes. Entomopathogenic fungi are primarily believed to infect host insects through external cuticle penetration. Here, we report a new variety, Beauveria bassiana var. majus (Bbm), that can infect insects through the previously unrecognized foregut. Dual routes of infection significantly accelerate insect mortality. The pH-responsive transcription factor PacC in Bbm exhibits rapid upregulation and efficient proteolytic processing via PalC for alkaline adaptation in the foregut. Expression of PalC is regulated by the adjacent downstream gene Aia. Compared to non-enteropathogenic strains such as ARSEF252, Aia in Bbm lacks a 249-bp fragment, resulting in its enhanced alkaline-induced expression. This induction promotes PalC upregulation and facilitates PacC activation. Expressing the active form of BbmPacC in ARSEF252 enables intestinal infection. This study uncovers the pH-responsive Aia-PalC-PacC cascade enhancing fungal alkaline tolerance for intestinal infection, laying the foundation for developing a new generation of fungal insecticides to control destructive insect pests.

A Review on Design and Fabrication of Mechanically Operated Gate System

Entryways have become increasingly common in private settings, serving as entrances to enclosed spaces or gaps in fences to regulate passage. Nowadays, many entry gates are operated by automated systems, boasting various advanced features. The demand for automated or mechanically operated doors has been steadily rising. The described automatic gate system is designed to automate entrances to parking lots of residential properties, businesses, and public car parks. It utilizes a mechanically controlled mechanism comprising a base plate connected to an L-shaped rod, which is in turn attached to a pulley fixed on the gate's main body. This setup enables the gate to be raised by spinning the pulley, and it gradually descends with the assistance of a car boot shock absorber when the vehicle's load is removed. This system ensures continuous gate opening and closing without the need for an external power source. While operating the gate manually reduces the burden on individuals, it is designed to function automatically through mechanical movements without requiring external control. Keywords: Mechanical Operated Gate, Pulley, Base plate, Boot shock

Design and Development of Smart Parking System Using RFID Technology

We can now access all non- interenet things from anywhere through Internet of thinking IoT and it made easy to access. People must grow with the technology. With the Parking what we have now, People are facing difficulties to park their Vehicles in their Parking Space in Cities. In Our study we designed a Smart Vehicle Parking, with which people can know about the nearest Parking Slot it will give the data of available Parking Slots in respective parking Space. Using the Smart Vehicle Parking People can save their time by finding available Slots So they don’t have to spend time and it they don’t have to travel already filled parking lots in a parking areas. It also reduces fuel consumption which is good for atmosphere.Radio-frequency identification (RFID) is a device that allow us to store the data in RFID tag ,it is an automated identifying technique s for the remote retrieval or transponders.An RFID tag is a radio-frequency identification and tracking device that is used to integrated into a product, animal, or human, as it is using radio waves for tracking. Some RFID tags are read from many meters away . We can use RFID technology in malls and Buildings for vehicle Parking . This Vehicle Parking System is having RFID card,RFID Reader a LED display board, vehicle counter and a audrino micro-controller. When a car is to be parked , RFID reader which is installed near the entry gate will read the RFID card. When a vehicle enter a parking slot it will open the gate and the the vehicle will be identified and when it takes a Parking slot it will be displayed in the LED Display and the available spot will reduce by and occupied slot will be increased by 1. When the same vehicle leave the parking slot, the available slot will increase by 1 and occupied slot will reduce by one . We can also book and reserve a parking slot for us, when there are no parking slot availabe it will notify us as no Parking slot is available

Smart Car Parking System Using Raspberry Pi

This paper deals with a simple smart vehicles parking system by using basic components like micro controller, IR Sensors, Servo Motor, LCD etc. Parking, offered in malls, shopping areas, etc., are provided with help of a person which is very time consuming. Because of this, we created a straightforward vehicle parking system that uses IR sensors installed at parking spaces to detect the presence of parked vehicles, an LCD display board that shows the number of vacant and occupied parking spaces, and an LCD display board that shows the number of parking spaces currently available. Therefore, the research methodology work solution creates and implements an experimental setup that will regulate traffic in parking areas while also informing drivers about free spots. The main function of the servomotor is to permit and prohibited vehicles in and out of indoor spaces by opening and turning off the entry or exit barrier according to the data obtained from the IR sensors which are present on the entry gate, exit gate, parking spots.

Conversion of Host Cell Receptor into Virus Destructor by Immunodisc to Neutralize Diverse SARS-CoV-2 Variants.

The decreasing efficacy of antiviral drugs due to viral mutations highlights the challenge of developing a single agent targeting multiple strains. Using host cell viral receptors as competitive inhibitors is promising, but their low potency and membrane-bound nature have limited this strategy. In this study, the authors show that angiotensin-converting enzyme 2 (ACE2) in a planar membrane patch can effectively neutralize all tested severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that emerged during the COVID-19 pandemic. The ACE2-incorporated membrane patch implemented using nanodiscs replicated the spike-mediated membrane fusion process outside the host cell, resulting in virus lysis, extracellular RNA release, and potent antiviral activity. While neutralizing antibodies became ineffective as the SARS-CoV-2 evolved to better penetrate host cells the ACE2-incorporated nanodiscs became more potent, highlighting the advantages of using receptor-incorporated nanodiscs for antiviral purposes. ACE2-incorporated immunodisc, an Fc fusion nanodisc developed in this study, completely protected humanized mice infected with SARS-CoV-2 after prolonged retention in the airways. This study demonstrates that the incorporation of viral receptors into immunodisc transforms the entry gate into a potent virucide for all current and future variants, a concept that can be extended to different viruses.

An ingredient co-occurrence network gives insight into e-liquid flavor complexity.

Part of the appeal of e-cigarettes lies in their available flavors. To achieve attractive flavors, e-liquids contain many different flavoring agents, which allow many flavoring combinations. To advance our knowledge of e-liquid flavors and compositions and to evaluate the effect of legislation, we determined whether there are ingredient combinations that are frequently used together. We used e-cigarette ingredient data from the European Common Entry Gate system (EU-CEG) as available on 31 December 2022. In e-liquids, we found 214 ingredient pairs with a co-occurrence odds ratio greater than 10. Together, these consisted of 62 unique ingredients. Network analysis revealed that ingredients were grouped together based on their flavor and/or chemical structure. We identified two densely connected regions (clusters) in the network. One consisted of six ingredients with sweet-vanilla-creamy flavors. The second cluster consisted of 13 ingredients. While some of these have fruity flavors, others, such as alkyl carboxylic acids and dimethyl sulfide, are known to have unpleasant flavors. Additional data and literature analyses indicated that alkyl carboxylic acids can contribute to a creamy and sweet-fruity taste, whereas dimethyl sulfide can contribute to a more refined fruity taste. These results exemplify that the flavor of e-liquids is not just the sum of its parts. Big data analyses on product data can be used to detect such patterns, but expert knowledge and additional data are needed for further interpretation. Monitoring of e-liquid flavors as well as ingredients will remain important to regulate e-liquid product attractiveness.

The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression

The transcriptional antisilencer VirB acts as a master regulator of virulence gene expression in the human pathogen Shigella flexneri. It binds DNA sequences (virS) upstream of VirB-dependent promoters and counteracts their silencing by the nucleoid-organizing protein H-NS. However, its precise mode of action remains unclear. Notably, VirB is not a classical transcription factor but related to ParB-type DNA-partitioning proteins, which have recently been recognized as DNA-sliding clamps using CTP binding and hydrolysis to control their DNA entry gate. Here, we show that VirB binds CTP, embraces DNA in a clamp-like fashion upon its CTP-dependent loading at virS sites and slides laterally on DNA after clamp closure. Mutations that prevent CTP-binding block VirB loading in vitro and abolish the formation of VirB nucleoprotein complexes as well as virulence gene expression in vivo. Thus, VirB represents a CTP-dependent molecular switch that uses a loading-and-sliding mechanism to control transcription during bacterial pathogenesis.

Acceleration of Protein Degradation by 20S Proteasome-Binding Peptides Generated by In Vitro Artificial Evolution.

Although the 20S core particle (CP) of the proteasome is an important component of the 26S holoenzyme, the stand-alone 20S CP acts directly on intrinsically disordered and oxidized/damaged proteins to degrade them in a ubiquitin-independent manner. It has been postulated that some structural features of substrate proteins are recognized by the 20S CP to promote substrate uptake, but the mechanism of substrate recognition has not been fully elucidated. In this study, we screened peptides that bind to the 20S CP from a random eight-residue pool of amino acid sequences using complementary DNA display an in vitro molecular evolution technique. The identified 20S CP-binding amino acid sequence was chemically synthesized and its effects on the 20S CP were investigated. The 20S CP-binding peptide stimulated the proteolytic activity of the inactive form of 20S CP. The peptide bound directly to one of the α-subunits, opening a gate for substrate entry on the α-ring. Furthermore, the attachment of this peptide sequence to α-synuclein enhanced its degradation by the 20S CP in vitro. In addition to these results, docking simulations indicated that this peptide binds to the top surface of the α-ring. These peptides could function as a key to control the opening of the α-ring gate.

Pelatihan Cooperate Learning Melalui Media English Exploratory Vacation pada Mahasiswa Sadar Wisata Palembang Darussalam

Tourism is the entry gate to revive the economy after the pandemic ends. Many tourists treat longing that has long been constrained by the situation brought about by covid 19. In increasing the confidence of tourists visiting Palembang City, students who are members of the Palembang Darussalam Tourism forum hold English proficiency training with a cooperative learning method that aims to further increase confidence when speaking English. This is in line with the large number of foreign tourists who enter Palembang City to travel or do business. This community service activity is focused on improving the English language skills of students who are members of the Palembang Darussalam tourism aware student forum. This activity was carried out via zoom meeting by providing information about tourism in Palembang City and directly practicing speaking English.

EUREST-RISE: An innovative networking and training project on European Tobacco Control

Abstract Aim and objective The world's leading contributor to avoidable illness and premature death is thought to be smoking and other tobacco use. An integrated set of research projects are suggested as part of EUREST-RISE to aid in the training of researchers in the analysis of tobacco product systems, their processes and functions, and the relationships between important design elements and their evaluation in user populations. Methods Currently in its first phase of execution, EUREST-RISE is a Marie Sklodowska Curie Action (MSCA) funded under Grant Agreement (Number:101008139). In the framework of particular research objectives addressed within tasks of each WP, interdisciplinary knowledge transfer will be achieved through the intersectoral secondment of researchers and their reintegration to their home institution. Results By offering a special framework for collaboration on joint research and innovation projects in three areas, EUREST-RISE expects to achieve its goal of developing a cohort of scholars with cutting-edge practical training in European tobacco product regulatory science. A) Exchange of information about the use of big data bases to address tobacco control concerns from a product-level or population-level viewpoint, including EU Common Entry Gate product-level data and European secondary population-based databases B) offer research and training on the application of sensory and chemical analyses for the evaluation of flavors in heated tobacco products, e-cigarettes, and tobacco products C) train researchers in advocacy activities and policy integration, facilitate training in peer to peer communication, and training on communication to media and the general public. Conclusions A network of excellence in European tobacco product regulation science is anticipated to be created via the EUREST-RISE initiative. Key messages • Conflicts of Interest: None. • Funding: The EUREST-RISE Project takes place with the financial support of the European Union, under a Grant Agreement (Number:101008139, PI: Dr Vardavas).

The Impact of the Resort-Based Borobudur Highland Master Plan as an Integrated Tourism Area

Tourism in Indonesia has quite good potential and is one of the sectors that spurs the economy in Indonesia. Development of Borobudur Highland which is included in the five super priority tourist destinations. One of them is the construction of hotels and resorts in the Menoreh Hills, Purworejo Regency, Central Java. The master plan was made as a tourism area plan under the Borobudur Authority Agency (BOB) covering an area of ​​309 hectares (ha). This master plan consists of five zones, namely the exclusive resort zone, the entry gate zone, the adventure zone, the cultural zone and the extreme zone. There are three themes in the development of this area, namely ecotourism, cultural tourism and adventure tourism. The method used is using descriptive qualitative. Data collection techniques through interviews, observation, and documentation. The data analyzed using the SWOT matrix. The results of this research can increase the existence of tourism objects in the Purworejo and Kulonprogo areas to international class that are sustainable, educative, and provide new breakthroughs that have an impact on investment in employment and local products around the area. The threats that can occur are changes in rainwater catchment areas to become prone to landslides, loss of springs, damage to ecosystems due to logging of trees, and disruption of existing flora and fauna habitats.

Circumventing cigarette regulation: Product characteristics of cigarette-like cigarillos on the Dutch market.

Cigarillos have been reported to provide an alternative to cigarettes with a characterizing flavor, which are banned in the European Union. Additionally, they are cheaper than cigarettes. To better inform policy making, we wanted to gain better insight into the market situation for cigarillos in the Netherlands. We analyzed product data notified by manufacturers via the European Common Entry Gate system (EU-CEG), as extracted on the 1 June 2022. First, we identified parameters that allowed classifying cigarillos into cigar-like, cigarette-like and intermediate-type cigarillos. Next, we compared product characteristics for these groups. We identified five parameters that allowed classifying cigarillos into subtypes: product weight, filter presence, leaf tobacco percentage in the product, flue-cured tobacco percentage in the product, and the number of flavorings. Almost a quarter (71/285) of the cigarillos on the Dutch market were classified as cigarette-like. Compared to other cigarillo types, these have a high number of flavorings (average n=43), including many sweet and fruity flavorings. The package types of cigarette-like cigarillos resemble those of cigarettes. However, 85% of the cigarette-like cigarillos are available in smaller pack sizes than allowed for cigarettes. When comparing data over the period 2019-2022, we found a decrease in the number of cigar-like cigarillos and an increase in the number of cigarette-like cigarillos, which hints at a shift in the market composition. Cigarette-like cigarillos can provide a way to evade cigarette regulation. Moreover, their characteristics make them attractive for consumers, including young people. Regulators should consider amending regulations to close the regulatory loopholes that allow evading tobacco legislation.

Evaluation of the influence of Inosine pranobex on the matrix protein system in patients with chronic viral cervicitis

The reproductive potential of both women and men is declining every year. Many factors contribute to the violation of the reproductive function – chemical, physical, mechanical, psychogenic, however, biological factors have the most pronounced effect on reproduction. Chronic viral cervicitis can be not only the cause of infertility and reproductive losses, but also the development of intraepithelial dysplasia, as well as cervical cancer. PVI, as a monoinfection, is quite rare along with HPV. Other UGIs (urogenital infections) act as common routes of transmission and entry gates. The most common association with PVI is herpesvirus infection. An increase in MMP, both systemically and at the local level, may indicate a violation of cell modeling processes, which contributes to the development of autoimmune inflammation with further destruction of the tissues of the reproductive tract. Activation of MMP promotes the release of HSV from the nerve ganglia and reactivation of the infection. Therapy for HPV and HVI (herpes virus infections) are debatable. There is no single standard of treatment, but there are a number of drugs that have antiviral and immunomodulatory effects. Currently, there are no studies on the dynamics of the effect of HPV and HSV infection on the state of MMPs and TIMPs during Inosine pranobex therapy. Objective: to evaluate changes in matrix metalloproteinases 2 and 9 and their tissue inhibitors types 1 and 2 in patients with human papillomavirus and herpes infections after Inosine pranobex therapy. 6 patients with papillomavirus and herpetic infections were examined and treated with drugs containing the active ingredient Inosine pranobex. The levels of MMP-2, MMP-9 and TIMP-1, TIMP-2 in blood serum were determined using specific reagents from R&D Diagnostics Inc. (USA). The dynamics of indicators in the blood serum of patients with PVI showed a decrease in the level of MMP-2, MMP-9, TIMP-1 with a simultaneous increase in TIMP-2 relative to the values before therapy. In patients with PVI and HVI, Inosine pranobex therapy showed a decrease in MMP-2 and MMP-9 levels, no changes in the content of TIMP-1, but an increase in the serum content of TIMP-2. Prior to the use of therapy, an increase in the ratio in the main groups in comparison with the control group was found, however, the largest increase was found in the group with the association of infections. After therapy, positive dynamics was established in the main groups. Thus, the ratio in group I decreased and became equal to the control values. In the II group of patients, the ratio, despite the decrease, remained higher than the control values and higher in comparison with the I group of women.