Coupling Microalgae-based Biochar with MBGS Enhances Microbial Synergy and Multi-Pollutant Removal from Saline Aquaculture Wastewater.

Bioaccumulation and adverse effects of three antibiotics in lettuce under environmentally-relevant levels.

Organic photoelectrochemical transistor (OPECT) holds significant promise for bioanalysis, but its application is constrained by dual-voltage requirements. Here, we constructed a Bi-MOF@CdS@Au/DNA-gated self-powered OPECT for enrofloxacin (ENR) detection by integrating a solar cell between the source and drain electrodes. The presence of ENR initiates specific binding with the aptamer, which induces aptamer dissociation from the electrode surface and, consequently, enhances electron transfer efficiency. This enhances the photogenerated gate voltage and promotes cation migration, leading to a marked decrease in channel current (IDS). The platform demonstrates good analytical capabilities, showing a wide linear detection range from 100 pg/mL to 100 μg/mL (R2 = 0.993) with a detection limit of 0.568 pg/mL (S/N = 3). This work demonstrates that the self-powered OPECT provides a promising platform for environmental monitoring applications.

Long-lived photogenerated carriers enabled by S-scheme heterostructured HOF-on-hollow MOF core-shell nanoarchitectures for boosting self-powered photoelectrochemical sensor to detect enrofloxacin.

The gut as a reservoir of drug-resistant pathogens: Mechanisms of ENR-driven horizontal gene transfer in aquaculture.

Synergistic surface plasmon resonance effect of Ag and S-scheme heterojunction in Ag/AgBr/Bi24O31Cl10 towards enhanced enrofloxacin removal: Performance, degradation pathways and synergistic mechanism.

Biochemical and molecular stress responses to enrofloxacin in Mytilus galloprovincialis: transient or lasting effects?

Electrochemical behavior and electro-aging of microplastics: interaction with enrofloxacin and Pb.

Detection of antibiotic-resistant Escherichia coli using surface-enhanced Raman spectroscopy and infrared spectroscopy.

Mechanistic insights and application of SS/MS/C/CaO₂ composite for the simultaneous removal of ammonia nitrogen and multiple antibiotics.

Smartphone-assisted multicolor aptamer-lateral flow assay using trimetallic nanoprobes and enzyme-free signal amplification for ENR detection.

Simultaneous Removal of Nitrate and Typical Antibiotics Using Fe/MS/C Composite: Performance, Mechanism, Ecological Impact, and Practical Application.

Fixed-bed catalytic antibiotics detoxification through singlet oxygen-mediated nonradical oxidation: Mechanisms and long-term performance.

Toxic effects of enrofloxacin on grass carp (Ctenopharyngodon idellus): an ecological risk assessment based on cross-system regulatory mechanisms and network toxicology.

Efficient removal of nitrogen, phosphorus and antibiotics in freshwater aquaculture wastewater using a novel hybrid aeration biological filter.

Dual nuclease-amplified sensitive biosensor for enrofloxacin detection using a DNase I-assisted CRISPR/Cas12a (CRISPR-DNase I) system.

A ratiometric fluorescent probe for the detection of fluoroquinolones and tetracyclines.

Mixture predicted no-effect concentrations of surfactants and antibiotics: Modeling based on experimental testing.

A dual-mode portable biosensor based on photothermal-pyroelectric-electrochromic effects for versatile detection of enrofloxacin.

Planktonic microalgae deploy multifaceted responsive and adaptive strategies against anthropogenic pollutants; however, current understanding of antibiotic resistance mechanisms remains predominantly focused on intrinsic physiological adaptations. While microalgae maintain intimate relationships with the phycosphere microbiome, the ecological roles of these associated microbes in mediating host adaptation to polluted environments are inadequately characterized. We identified a phycosphere microbiome-involved antibiotic resistance mechanism in Dictyosphaerium sp., a pollution-tolerant Chlorophyta microalgae exhibiting remarkable enrofloxacin (ENR) tolerance. Microalgal growth displayed initial inhibition followed by significant promotion under 5mg/L ENR exposure. This resilience was associated with the restructuring of phycosphere microbiome, characterized by Porphyrobacter enrichment and functional enhancement of algal fitness-promoting pathways, including upregulation of cobalamin biosynthesis genes (log2FC = 7.76) and a 33.3-fold increase in extracellular B12 accumulation. Consequently, we isolated the ENR-selected microbial taxa to elucidate their roles in microalgal stress adaptation. Co-culturing axenic Dictyosphaerium sp. with Porphyrobacter enhanced microalgal growth by 36.5% after 8-day ENR exposure, whereas non-dominant bacteria exhibited negligible effects. Based on the transcriptomic and metabolomic analyses of the algal system when Porphyrobacter was dominant, we subsequently compared the growth of axenic microalgae with and without B vitamin (B1, B6, B7, B12) supplementation. Experimental validation demonstrated the pivotal role of B12-producing Porphyrobacter in enhancing microalgal ENR adaptation through (i) stimulating extracellular polymeric substances production and subsequently enhancing ENR removal via EPS-mediated adsorption and (ii) alleviating intracellular oxidative stress via elevating superoxide dismutase and peroxidase activities and reducing malondialdehyde levels. Additionally, this B12-producing bacteria/B12-mediated adaptability exhibited cross-species conservation, improving ENR resistance in Chlorella vulgaris and Scenedesmus quadricauda, with analogous protection observed under ciprofloxacin and norfloxacin exposures. Collectively, our findings establish stress-induced enrichment of B12-producing Porphyrobacter within the phycosphere microbiome as a pivotal mechanism underlying microalgal antibiotic adaptation. This insight facilitates the rational development of microalgae-microbiome systems for enhanced wastewater treatment and sustainable bioproduction, with applications in aquatic feed supplementation, biofuel production, and biofertilizer development. Video Abstract.