Enriched Diet Research Articles

PROtein enriched MEDiterranean diet to combat undernutrition and promote healthy neuroCOGnitive ageing in older adults: The PROMED-COG consortium project.

Dementia is a major public health challenge owing to its increasing prevalence and recognised impact on disability among older adults. Observational data indicate that weight loss is associated with increased dementia risk of 30%–40% and precedes a diagnosis of cognitive impairment or dementia by at least one decade. Although relatively little is known about the mechanisms of unintentional weight loss in dementia, this provides a window of opportunity to intervene with strategies to counteract undernutrition and delay, or prevent, the onset of dementia. This article provides an overview of the PROMED‐COG project and associated work packages. The project aimes to (1) strengthen the epidemiologic evidence to better understand the potential benefits of combating undernutrition for healthy neurocognitive ageing; (2) increase scientific knowledge on the balance between a protein enriched Mediterranean diet (PROMED) and physical exercise to prevent undernutrition and promote healthy neurocognitive ageing, and generate data on mechanistic pathways; (3) stimulate collaboration and capacity building for nutrition and neurocognitive ageing research in Europe; and (4) develop public and practice recommendations to combat undernutrition and promote healthy neurocognitive ageing in older adults. Findings will provide new and critical insights into the role of undernutrition in neurocognitive ageing, how this role can differ by sex, genetic risk and timing of undernutrition exposure, and how modifications of dietary and physical activity behaviour can reduce the burden of undernutrition and neurodegeneration. The research outcomes will be useful to inform policy and practice about the dietary guidelines of older people and provide insight to industry for the development of food‐based solutions to prevent undernutrition.

Consumption of Farmed Fish, Fed with an Olive-Pomace Enriched Diet, and Its Effect on the Inflammatory, Redox, and Platelet-Activating Factor Enzyme Profile of Apparently Healthy Adults: A Double-Blind Randomized Crossover Trial.

A fish-rich diet has a beneficial effect on cardiovascular health. The platelet activating factor (PAF) is involved in the development of atherosclerosis, and in vitro results support the regulating action of bioactive nutrients on PAF metabolism. The purpose of this study is to examine whether the consumption of farmed fish fed with an olive-pomace enriched diet (EF) affects PAF metabolism and the markers of inflammation and oxidative stress compared to the consumption of conventionally fed farmed fish (CF). Thirty apparently healthy adults completed a randomized double-blind crossover trial, during which they consumed both CF and EF twice a week for 8 weeks with a six-week washout period in between. The activities of PAF acetylhydrolase (PAF-AH), lysoPAF acetyltransferase (lysoPAF-AT), DTT-insensitive CDP-choline: 1-alkyl-2-acetyl-sn-glycerol-choline-phosphotransferase (PAF-CPT) in leukocytes, and lipoprotein-associated phospholipase A2 (LpPLA2) in serum were determined. The quantities of interleukin-6 (IL-6), high sensitivity C-reactive protein (hsCRP), oxidized LDL (ox-LDL), thiobarbituric acid-reactive substances (TBARS), and glutathione peroxidase (GPx), as well as the serum oxidation, were also determined. Both types of fish exerted similar effects as there were no statistically significant differences between the two interventions except for an elevated PAF-CPT and reduced arachidonic acid (AA) in the red blood cell (RBC) membrane lipids after the EF intake.

Nifuroxazide modulates hepatic expression of LXRs/SR-BI/CES1/CYP7A1 and LDL-R and attenuates experimentally-induced hypercholesterolemia and the associated cardiovascular complications

Hyperlipidemia is a serious disorders affecting the metabolism of fats in the human body, and it is usually associated with some serious cardiovascular complications increasing the risk for sudden death. Nifuroxazide (NFR) is an oral nitrofuran antibiotic that has long been used for management of diarrhea and recently various recent out merging valuable therapeutic impacts were reported. The current study sought the concept of repositioning nifuroxazide in management of hyperlipidemia. Hyperlipidemia was induced in male rabbits using cholesterol enriched diet for 9 weeks and starting from the beginning of 5th week; NFR (100 and 300 mg/kg) were administered once daily for the further 5 weeks; till the end of the 9th week of the experiment. NFR significantly recovered balanced lipid profile as serum cholesterol, total glycerides, LDL significantly declined with significant elevation in serum HDL. Meanwhile, serum LDH, CK, ALT and AST activities were significantly corrected. These biochemical changes were correlated with significant improvement in the histopathological examination of hepatic, cardiac and aortic specimen with decreased expression of CD68 and Ki67 in the myocardium and the aorta implying retraction in macrophages' infiltration and tissue regeneration. Myocardial specimen confirmed significant recovery with preservation of cardiac muscle fibers. Aortic specimen confirmed retraction in the aortic thickness and fewer deposition of fat globules. In conclusion, NFR attenuated experimentally-induced hyperlipidemia with significant recovery of serum profile and tissue necrotic changes. The histopathological examination of hepatic, myocardial and aortic specimen confirmed the onset of tissues' recovery alongside biochemical improvement.

Parental effects of Bt toxin and vitamin A on Helicoverpa armigera.

The increase in the area cultivated with vitamin-enriched transgenic crops producing Bt toxin raises the question of whether the addition of vitamins will in any way mitigates the effect of the toxin on the phytophagous insects that feed on those crops. On the other hand, the parental effect that feeding on these enriched transgenic crops may have on the offspring of the phytophagous that survive on them is not well known. In this work, the effect of vitamin A (β-carotene) addition to diets with or without Bt toxin on Helicoverpa armigera larvae and their offspring was determined. The addition of vitamin A did not have any beneficial effect either for the larvae fed on enriched diets nor for their offspring. However, parental effects due to dietary feeding with the toxin were detected since adults from larvae fed on the Bt diet had higher mating success than those fed on the toxin-free diet, although there were no differences on the fertility of mated females regardless of whether their previous larvae fed on the Bt or non-Bt diet. A certain adaptive effect to the toxin was also noted since the mortality of larvae whose previous generation fed on diet with Bt was lower than that of the larvae that came from larvae fed on a non-Bt diet. It would be interesting to determine if H. armigera adults prefer to mate and lay eggs in the same type of crops in which they have developed or if feeding on different crops, such as corn or alfalfa, causes different paternal effects on the offspring. These aspects can be of great importance in the development of resistance of this species to the Bt toxin.

Effects of Illicium verum Hook. f. (Chinese herb) enriched diet on growth performance, immune response and disease resistance in Catla catla [Hamilton] fingerlings against Aeromonashydrophila

Our present study investigates the effects of Illicium verum enriched diet on growth performance, immune response and disease resistance in Catla catla fingerlings against Aeromonas hydrophila. Experimental groups, i.e., infected C. catla were fed with 5 g/kg, 10 g/kg and 15 g/kg of I. verum diet for a period of 30 days showed a significant increase in their final body weight (FBW) over control. There was a significant increase (p < 0.05) in total weight gain (TWG), specific growth rate (SGR), feed conversion rate (FCR), protein efficiency ratio (PER), condition factor (CF) and survival rate (SR) in 10 g/kg and 15 g/kg groups compared to control; also from 31 to 60 days these groups showed an enhanced (p < 0.05) complement activity, phagocytic activity, respiratory burst activity and lysosome activity. The experimental group fed with 10 g/kg of I. verum diet showed the least cumulative mortality rate (5%) compared to 5 g/kg (30%) and 15 g/kg (15%) experimental groups and in control (10%) and negative control (75%). Our present study highlights that challenged fish fed with the mid dosage of 10 g/kg showed a significant increase in overall growth performance, non susceptibility to diseases and tolerance against A. hydrophila in C. catla compared to other experimental groups including the group given with high dosage of 15 g/kg. Thus our results suggest 10 g/kg as the optimal dosage of I. verum that can be used to enrich the fish diet for a better performance and effective immunostimulation in commercially important C. catla for its sustainable culture and is reported for the first time.

Integrated biomarker responses in European seabass Dicentrarchus labrax (Linnaeus, 1758) chronically exposed to PVC microplastics

Few studies evaluated long-term effects of polyvinyl chloride (PVC) microplastics (MPs) ingestion in fish. The present study aimed to investigate the integrated biomarker responses in the liver and blood of 162 European seabass, Dicentrarchus labrax, exposed for 90 days to control, virgin and marine incubated PVC enriched diets (0.1 % w/w) under controlled laboratory condition.Enzymatic and tissue alterations, oxidative stress, gene expression alterations and genotoxicity were examined. Additives and environmental contaminants levels in PVC-MPs, control feed matrices and in seabass muscles were also detected.The results showed that the chronic exposure at environmentally realistic PVC-MPs concentrations in seabass, cause early warning signs of toxicological harm in liver by induction of oxidative stress, the histopathological alterations and also by the modulation of the Peroxisome proliferator-activated receptors (PPARs) and Estrogen receptor alpha (ER-α) genes expression. A trend of increase of DNA alterations and the observation of some neoformations attributable to lipomas suggest also genotoxic and cancerogenic effects of PVC.This investigation provides important data to understand the regulatory biological processes affected by PVC-MPs ingestion in marine organisms and may also support the interpretation of results provided by studies on wild species.

Nutritional Properties of Milk from Dairy Ewes Fed with a Diet Containing Grape Pomace.

The aim of the present study was to evaluate the effects of a diet containing a 10% of grape pomace (GP) on the milk yield, chemical-nutritional characteristics, total phenolic compounds (TPCs), antioxidant activity (AOA), fatty acids and proteins profile of dairy ewe’s milk. Forty-six ewes were dived into two groups: a control group (Ctrl), fed a standard diet, and an experimental group (GP+), whose diet was supplemented with 10% of GP on dry matter. The trial lasted 60 days and milk samples were collected and analyzed at the beginning (T0) and after 60 (T60) days. Dietary enrichment with GP did not affect the yield and the chemical composition of the milk. TPCs and AOA were not affected by the diet. After 60 days, the diet induced an increase in monounsaturated fatty acids (MUFA) and a decrease in medium chain saturated fatty acids (MCSFA), but the total saturated fatty acids (SFA), polyunsaturated fatty acids (PUFA), short chain saturated fatty acids (SCSFA) and long chain saturated fatty acids (LCSFA) were not modified. A decrease in the C14 desaturation index and an increase in the C18 index were also detected. Total caseins and whey protein were not affected by GP, even if a lower content of k-casein in GP+ milk compared to Ctrl milk was observed on the 60th day. The results of the present study suggest that 10% of GP can be included in the diet of lactating ewes without modifying milk gross composition but inducing significantly changes the fatty acid profile.

Effects of dietary astaxanthin enrichment on enhancing the colour and growth of red tilapia, Oreochromis sp.

The aim of this research was to examine the effects of dietary inclusion of astaxanthin from Haematococcus pluvialis in commercial fish feed on the colour and growth of red tilapia, Oreochromis sp.. A 6-week feeding trial was conducted at concentration of astaxanthin at 0% (T1), 4% (T2), 8% (T3) and 12% (T4) in feed formulation to examine the growth performance, colour pigmentation on skin, texture analysis and carotenoid contents. Results showed an increment of certain growth parameter in fish fed with astaxanthin enriched diet. Increased in weight gain (WG), protein efficiency ratio (PER) was obtained in fish fed with astaxanthin enriched compared with control. All treatments with astaxanthin-enriched provided higher redness (a*) to skin than negative control that directly influence the colour of the fish. It can be concluded that inclusion of astaxanthin from green algae into commercial fish feed can be used as an alternative natural carotenoid source in Oreochromis sp. diets to ensure good pigmentation, hence improving the skin colour for more appealing and subsequently increase the market value of the fish.

Metabolic and Microbiome Alterations Following the Enrichment of a High-Fat Diet With High Oleic Acid Peanuts Versus the Traditional Peanuts Cultivar in Mice.

A new Israeli-developed peanut cultivar, “Hanoch-Oleic” (HO), uniquely contains enlarged oleic acid contents and was designed to confer additional beneficial effects over the traditional cultivar, “Hanoch” (HN). This work elucidates metabolic changes and microbiota adaptations elicited by HO addition to a high-fat diet (HFD). Male C57BL/6 mice were fed for 18 weeks with a normal diet or a HFD with/without the addition of HN (HFDh) or HO (HFDo). Body-weight did not differ between HFD-fed mice groups, while liver and adipose weight were elevated in the HFDh and HFD groups, respectively. Insulin-sensitivity (IS) was also decreased in these groups, though to a much greater extent in the traditional peanuts-fed group. Modifications in lipids metabolism were evident by the addition of peanuts to a HFD. Liver inflammation seems to return to normal only in HFDh. Peanuts promoted an increase in α-diversity, with HFDo exhibiting changes in the abundance of microbiota that is primarily associated with ameliorated gut health and barrier capacity. In conclusion, the HO cultivar appears to be metabolically superior to the traditional peanut cultivar and was associated with an improved inflammatory state and microbial profile. Nevertheless, IS-negative effects reinforced by peanuts addition, predominantly NH, need to be comprehensively defined.

Abstract 5811: Ovarian cancer cell fate is regulated by the balance between saturated and unsaturated fatty acids

Abstract Background: Fat represents an important source of energy for ovarian cancer (OC) cells and is supplied either through import from the tumor milieu or via de novo lipogenesis. During fast tumor growth, when nutrients are scarce, lipogenesis becomes the primary source of fatty acids. Stearoyl-CoA desaturase (SCD), a rate-limiting enzyme in this pathway converts saturated (SFAs) into unsaturated fatty acids (UFAs) and is highly expressed in OC. The goal of this study was to determine how the balance between SFAs and UFAs tightly controlled by SCD, regulates OC cell survival and tumorigenicity. Methods: SCD was knocked down by shRNA or inhibited by using the small molecule CAY10566 and global effects on the lipidome and transcriptome were examined by targeted and untargeted lipidomics, stimulated Raman spectroscopy (SRS), and RNA sequencing. In OC cells in which SCD was blocked or knocked down, the effects of exogenous SFAs and UFAs on cell survival and endoplasmic reticulum (ER) stress pathway were assessed by using annexin V staining, XBP1 splicing assay and Western blotting of PERK/eIF2α/ATF4. Tumorigenicity was assessed by using an intraperitoneal (i.p.) xenograft model in nude mice. Results: RNA-seq analysis of SCD knockdown cells cultured under low serum conditions revealed activation of ER stress response pathways. Targeted lipidomics and SRS microscopy showed downregulation of UFAs vs. SFAs, while untargeted lipidomics discovered decreased fatty acyl chain plasticity among phosphatidylcholines. Activation of IRE1α/XBP1 and PERK/eIF2α/ATF4 axes was observed in cells accumulating SFAs. Stiff and disorganized ER membrane was detected by electron microscopy and Raman spectroscopy. Annexin V staining demonstrated apoptosis of OC cells under long-term mild ER stress or short-time severe ER stress induced by increased levels of SFAs. ER stress and apoptosis were rescued by addition of UFAs. In vivo SCD knockdown suppressed tumor growth and treatment with the SCD inhibitor CAY10566 reduced the number of metastases and the volume of ascites in mice fed with SFA enriched diet, but not in mice fed a balanced diet. Conclusion: Our data support that OC cells are highly susceptible to unbalanced intracellular SFAs/UFAs, undergoing ER stress and apoptosis in the presence of excessive intracellular SFAs. SCD inhibition coupled with a diet rich in saturated fats decreased cancer progression in vivo. These results support SCD as a key regulator of cancer cell fate during metabolic stress in growing tumors and point to new treatment strategies targeting lipid metabolism. Citation Format: Guangyuan Zhao, Yuying Tan, Horacio Cardenas, David Vayngart, Yinu Wang, Christina Ferreira, Ji-Xin Cheng, Daniela Matei. Ovarian cancer cell fate is regulated by the balance between saturated and unsaturated fatty acids [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5811.

Effects of dietary docosahexaenoic acid enrichment in Artemia feed on the growth, survival, and fatty acid composition of Pacific cod ( Gadus macrocephalus ) larvae

Effects of dietary docosahexaenoic acid enrichment in <i>Artemia</i> feed on the growth, survival, and fatty acid composition of Pacific cod ( <i>Gadus macrocephalus</i> ) larvae

"A designer diet layout for astronauts using a microbiome mediated approach."

Astronauts undergo space travel to bring scientific information to benefit humanity under various missions of space agencies such as NASA, European Space Agency, Indian Space Research Organization etc. During space missions, they encounter several stressors namely microgravity, fluid shifts, cosmic radiation, sleep deprivation and alteration in the circadian rhythm perturbing the quality of sleep. In addition, confined spaces makes pathogen interaction more likely if a pathobiont gets introduced into spacecraft. Microbiota is the first line оf resistаnсe tо vаriоus disorders and diseаses. It direсtly influenсes the biосhemiсаl, рhysiоlоgiсаl, аnd immunоlоgiсаl раthwаys. 'Gut microbiota' is essential for maintenance of healthy gut barrier functions. 'Dysbiosis' refers to perturbation of microbiota which is correlated with several metabolic and psychological disorders. Microbial metabolites are implicated in maintenance of human health. Investigations conducted on astronauts in international space missions and on analog terrestrial models have indicated a 'dysbiosis' of the gut microbiota associated with spaceflights. 'Dysbiosis' of the gut microbiome observed in astronauts has been implicated in immune dysregulation and a probiotic enriched diet is proposed to restore immune homeostasis. This article not just summarizes the state of art research on dysbiosis of the gut microbiome of astronauts, but also a diet mediated correction plan to restore their health especially during long term space missions. A characterization of microbial metabolites of the gut to enable administration of astronaut specific probiotic, postbiotic or synbiotic to alleviate space associated dysbiosis is proposed. It is also recommended that astronauts maintain a balanced nutritious diet throughout life to promote a resilient microbiota that is not perturbed by space missions. Further, a bioregenerative life support system wherein a probiotic may be produced in space station is proposed.

The Effects of Maternal Intake of EPA and DHA Enriched Diet During Pregnancy and Lactation on Offspring's Muscle Development and Energy Homeostasis.

EPA and DHA are n-3 long-chain polyunsaturated fatty acids with a diversity of health benefits on offspring. The objective of this study was to test the in vivo effect of maternal ingestion of EPA and DHA on fetal and offspring muscle development and energy balance. Two groups of female C57BL/6 mice were fed EPA and DHA enriched diet (FA) and diet devoid of EPA and DHA (CON) respectively throughout the entire period of gestation and lactation. Embryos at E13 and offspring at age of D1 and D21 were selected for sample collection and processing. No change in birth number and body weight were observed between groups at D1 and D21. Transient increase in the expression levels of myogenesis regulating genes was detected at D1 (p < 0.05) in FA group. Most of the expression of muscle protein synthesis regulating genes were comparable (p > 0.05) between FA and CON groups at D1 and D21. The significant increase in MHC4, and IGF-1 was not linked to increased muscle mass. A persistent increase in ISR expression (p < 0.05) but not in GLUT-4 (p > 0.05) was detected in offspring. Up-regulation of adipogenesis regulating genes was accompanied by increasing intramuscular fat accumulation in the offspring of FA group. Considerable increase in transcripts of genes regulating lipid catabolism and thermogenesis in liver (p < 0.05) was noticed in FA group at D21; whereas, only the levels of carnitine palmitoyl transferase 1A (Cpt1α) and Enoyl-CoA Hydratase And 3-Hydroxyacyl CoA Dehydrogenase (Ehhadh) increased at D1. Similarly, genes regulating lipolysis were highly expressed at D21 in FA group. EPA and DHA treatment promoted BAT development and activity by increasing the expression of BAT signature genes (p < 0.05). Also, maternal intake of EPA and DHA enriched diet enhanced browning of sWAT. Taken together, maternal ingestion of EPA/DHA may be suggested as a therapeutic option to improve body composition and counteract childhood obesity- related metabolic disorders and confer lifelong positive metabolic impact on offspring.

477-P: Vagal GLP-1–Mediated Reductions in Postprandial Lipids Are Sensitive to Fructose Feeding

Dyslipidemia is a common co-morbidity of insulin resistance, where chronic elevations in hepatic and intestinal lipoproteins are linked to cardiovascular disease. Postprandial production is regulated in-part by gut-derived hormones such as glucagon-like peptide (GLP) -1. Although degradation-resistant analogs of GLP-1 continue to work in diabetic individuals, none have examined whether GLP-1s endogenous neuroendocrine signalling pathways are affected by diet-induced dyslipidemia. Thus, we sought to test whether native GLP-1 could modulate postprandial lipids in a fructose-fed hamster model.Hamsters were fed either a chow or fructose-enriched (FF) diet for 14 days, then received a portal vein injection of either GLP-1 (7-36) or vehicle control to examine if they remained responsive to portal GLP-1. Separately, in hamsters fed either chow or a fat/fructose/cholesterol enriched (FFC) diet we performed nodose ganglia (NG) injections with GLP-1 (7-36) or vehicle. To recapitulate the loss of vagal GLP-1R signalling in the absence of dietary intervention we selectively deafferented GLP-1R containing NG neurons via injection of the cytotoxin saporin conjugated to exendin-4. After treatment, animals received a gavage of olive oil and an intraperitoneal injection of poloxamer to prevent lipoprotein clearance. Postprandial triglyceride-rich lipoproteins isolated via density ultracentrifugation were assessed via blood draws over a 6-hour period.Chow-fed hamsters saw significant reductions in post-prandial lipids after portal GLP-1 injection, whereas FF-fed hamsters were unaffected. Chow-fed hamsters were similarly responsive to GLP-1 (7-36) injection into the NG, displaying reduced postprandial lipids; however, FFC-fed hamsters showed no reduction in plasma lipids. Finally, selective deafferentation of NG GLP-1R-containing neurons caused significant rises in postprandial TG and TRL, underscoring the importance of GLP-1R insensitivity in states of metabolic disease. Disclosure S.S.Hoffman: None. D.C.Alvares: None. K.Adeli: None. Funding CIHR Foundation Grant

1307-P: Leptin Action in the Nucleus of the Solitary Tract

Obesity and diabetes are characterized by a disruption in energy balance and glucose homeostasis in association with leptin resistance. Leptin action in the hypothalamus regulates glucose levels and body weight, but whether other brain regions facilitate leptin action remain unclear. Here, we investigate whether the nucleus of the solitary tract (NTS) of the brainstem is a crucial site for leptin action in regulating glucose homeostasis in 3d high-fat (HF) -fed male rats, as recent studies report leptin activates leptin receptor in the NTS to regulate feeding. In this study, we performed brain stereotaxic bilateral surgery targeting the NTS followed one week later by catheterization of the left carotid artery and right internal jugular vein for infusion/sampling purposes. Rats that attained &amp;gt;90% of presurgical weight received 3d of Lard oil enriched HF diet and were fasted 4-6 hr before undergoing a 2min pancreatic (basal insulin) -euglycemic clamp studies together with tracer glucose infusion to assess glucose metabolism independent of changes in plasma insulin and glucagon levels. Leptin (91.7 ng/uL at 0.33 ul/hr) or saline was infused into the NTS throughout the duration of the clamp studies. Correct placement of the NTS catheter was verified by infusion of bromophenol blue dye through the brain cannula. For the first time, we found that leptin vs. saline infusion into the NTS markedly increased the exogenous glucose infusion [lep: 4.6±0.2 vs. sal: 1.3±0.4 mg/kg/min; p&amp;lt;0.005; n=12, 5] required to maintain euglycemia [lep: 137.5±2.2 vs. sal: 145±3.1 mg/dl; p&amp;gt;0.05; n=12, 5], during the clamps. The drop in plasma glucose levels was due to an inhibition of glucose production [lep: 4.9±0.1 vs. sal: 8.4±0.7 mg/kg/min; p&amp;lt;0.01; n=12, 5], but not an increase in glucose uptake [lep: 10.4±0.5 vs. sal: 9.7±0.3 mg/kg/min; p&amp;gt;0.05; n=12, 5]. These findings demonstrate that the NTS mediates leptin action on glucose homeostasis. Future studies are needed to investigate the NTS leptin signaling cascade in glucose homeostasis in obesity and diabetes. Disclosure K.Bruce: None. R.Li: None. Y.Lim: None. J.T.Yue: None. T.K.Lam: None. Funding Canadian Institutes of Health Research Foundation Grant (FDN-143204)

Effect of cow's milk with different PUFA n-6: n-3 ratios on performance, serum lipid profile, and blood parameters of grower gilts.

The concern with human health has increased the interest in producing foods enriched with polyunsaturated fatty acids (PUFA), directly or naturally, by inclusion in the animals’ diet. The positive effects such as antithrombotic, anti-inflammatory, and hypolipidemic have been observed in pigs and rats, used as human models for study. The present study evaluated the effect of cow’s milk with different lipid profiles on performance, serum fatty acid profile, biochemical analysis, and a complete blood count of gilts used as a human model. At 34 days, thirty gilts were equally distributed in three treatments. Experimental treatments were milk from cows without the oil supplementation (C), milk from cows fed an enriched diet with linseed oil (n-3), and milk from cows fed an enriched diet with soybean oil (n-6). Milk supplementation was performed until 190 days old, provided once in the morning. The n-3 and n-6 milk reduced the concentration of myristic acid in the blood and increased the leukocytes. Milk enriched with n-3 compared to n-6 reduced the stearic acid. In conclusion, milk with a better PUFA profile can reduce saturated fatty acids in the blood and alter the concentration of cells in the defense system.

Evaluation of UV-B Ameliorating Properties of Indigenous Plants Ashwagandha Withania somnifera (Dunal), Amla Emblica officinalis (Gaertn), and Prickly Chaff Flower Achyranthes aspera (L.) Supplemented Diets in Prior UV-B Exposed Catla catla

Ultraviolet B (UV-B) radiation is a potent environmental stressor and it severely affects the survival, growth, and physiology of aquatic organisms. The UV-B protective properties of three herbal ingredients enriched diets were evaluated in pre-exposedCatla catla. Fish (70.38 ± 1.18 g) were divided into five sets with three replicates each. Four sets were exposed to UV-B (80 µW/cm2) for an initial 10 days (20 min/day), and the fifth one remained unexposed (control). On day 11, feeding with enriched diets started. These diets were: diet 1 (D1) containingWithania somnifera(0.5%) root powder, diet 2 (D2) containingEmblica officinalis(1.25%) fruit powder, diet 3 (D3) containing seeds ofAchyranthes aspera(0.5%), and diet 4 (D4) control. There were two batches of D4 diet-fed fish, D4a, UV-B exposed, and D4b unexposed. Blood and tissue samples were collected on days 0, 7, 14, and 21 of feeding. The average weight reduced 26.32% in UV-B exposed catla compared to the unexposed one on day 0. Among the exposed fish, average weight was significantly higher in D3 compared to others throughout the study period. Significantly lower nitric oxide synthase (NOS) and higher thiobarbituric acid reactive substances (TBARS), carbonyl protein, superoxide dismutase (SOD), and heat shock proteins (Hsp) 70 and 90 were observed in D4a compared to the enriched diet fed catla. The NOS level was significantly higher in D3 on day 21. The TBARS level was significantly lower in D3 on days 7 and 14 and in D2 and D3 on day 21. The carbonyl protein, SOD, and Hsp70 levels were always significantly lower in D3 compared to others, and D2 followed D3 treatment. Hsp 90 was significantly lower in D2 compared to others on day 7 and in D3 on days 14 and 21. All three enriched diets helped the fish overcome the harmful effect of UV-B radiation, and the D3 diet-fed fish showed the best performance.

DESIGN AND ANALYSIS OF A DAILY DIET QUALITY FOR PRIMARY SCHOOL-AGED CHILDREN

The problem of designing a balanced diet for primary school-aged children as a very important lever to improve the health of children and adults is considered. It is demonstrated that children's nutrition differs from adult nutrition in several signs, in particular, it needs calcium in an easily digestible form, more nutrients and energy per unit of body weight, requires the use of gentle processing methods such as cooking, stewing and baking, removing some ingredients from the diet, etc. It is proposed to use mathematical models of the recipe and nutritional composition of rations for the design of rations, taking into account restrictions on the content of raw materials in the diet, ratios that ensure a high balance of fat, calcium, phosphorus and magnesium, as well as the conditions for the enrichment of diets with certain deficient nutrients regarding their daily needs. The choice of the target function for each diet model is justified based on preliminary calculations performed using the MS Excel spreadsheet. Using the proposed models, four single-use diets were developed for primary school-aged children - the first and second breakfast, lunch and dinner, which together form a balanced and varied daily diet. The rations are designed based on traditional or raw materials widespread in Ukraine with the involvement of only natural ingredients allowed by the current legislation of Ukraine for use in school meals. As a result of optimization carried out with the help of the method of built-in functions in the MathCAD system, optimal values ​​of raw ingredients contained in the diet were obtained. Based on the received data, the daily diet menu is composed. According to the results of testing the recipes of the proposed diet, their high quality was noted and the possibility of further use in children's and school nutrition was shown. According to the results of the analysis of the nutrients’ balance in the composition of the proposed single-use diets and the daily diet provided by the scientists, their high quality has been proven.

Effect of leaves and seeds of Achyranthes aspera as feed supplements on the immunological and stress parameters and related gene expressions of Asian catfish (Clarias batrachus).

The immunostimularory properties of Achyranthes aspera leaves and seeds supplemented feeds were evaluated in Asian catfish, Clarias batrachus (0.352±0.008 g). The experimental feeds contained 0.25% leaves (EFL1), 0.5% leaves (EFL2), 0.5% seeds (EFS) and 0% leaves or seeds (control feed; CF). In CF, leaves and seeds were absent. Fish were immunized with chicken-RBC after 60 days of feeding. The blood and tissue samples were collected on 7th, 14th and 21st days after immunization for various assays. The average weight of magur was significantly higher in EFS compared to the other treatments throughout the study period and EFL2 followed this group. Serum lysozyme level of fish was significantly higher in EFS on 7th and 14th days and in EFS and EFL2 on 21st day after immunization compared to the other treatments. Myeloperoxidase and nitric oxide synthase levels were always significantly higher in EFS diet fed fish compared to other treatments. The highest hemagglutination titer level was found in EFS throughout the study period. Thiobarbituric acid reactive substances (TBARS) and carbonyl protein levels were significantly lower in liver and kidney of enriched diets fed magur compared to CF treatment. TBARS and carbonyl protein levels were minimum in EFS diets fed fish. In EFS and EFL2 treatments, the expressions of TNF-α, iNOS and NF-kB were significantly higher compared to the CF group. A. aspera seeds and leaves showed significant immunostimulatory properties in Asian catfish fry.

FC 120: Lipid Changes as Early Indicator for Diabetes Induced Renal Pathology

Abstract BACKGROUND AND AIMS Diabetic nephropathy (DN), chronic loss of kidney function due to diabetes mellitus, is a common complication in diabetes which currently lacks effective long-term treatment. Microalbuminuria is now the earliest marker for DN, thereby disregarding renal changes that occur before this first indication of decreased kidney function. For better diagnosis and treatment strategies, it is important to study diabetes induced early renal changes. Renal-specific changes of the lipid metabolism could serve as an indicator for early kidney damage. Mass spectrometry imaging (MSI) allows to study the lipid metabolism in the context of tissue histology without the need for labeling (i.e. antibody or radioactive). Existing literature applying this technique focuses on later stages of diabetic kidney disease, thereby overlooking its potential to serve in an earlier stage of diagnosis. The aim of this project is to identify renal cell-specific lipid changes using MSI which could serve as damage markers for early diabetes induced renal pathology. METHOD To study diabetic renal changes, apolipoprotein E-knockout mice were treated with streptozotocin (STZ) and put on an enriched cholesterol diet to induce diabetes. After 12 weeks, both control (n = 4) and diabetic (n = 4) mice were sacrificed and kidneys were harvested for immunohistochemical and matrix assisted laser desorption/ionization (MALDI) MSI. Post-MSI immunofluorescent staining in combination with tissue morphology was used to identify different renal cell types. Analysis of MALDI-MSI data allows us to spatially segment the results per cell type for further statistical analysis of the metabolic MSI data (Figure 1A). RESULTS Two weeks after STZ induction, blood glucose levels of the diabetic mice were significantly elevated compared to control. Spatial segmentation analysis of the MSI data revealed that in both groups different renal cell types could clearly be distinguished based on their metabolic profile. Using the immunofluorescence-based cell type annotation, we zoomed in on various morphological regions of the kidney to specifically test for changes in lipid profiles. Here, the proximal tubular cells in the cortex and the outer stripe of outer medulla had the highest number of significantly changed lipids (Figure 1B). Focusing the analysis on these two groups of cells revealed specific molecular signatures to be discriminative between diabetes and control (Figure 1C). CONCLUSION Using MSI, we were able to identify renal cell type-specific differentially expressed lipids in diabetic mice compared to the healthy control group. The proximal tubular cells in the cortex and outer stripe of the outer medulla had the most altered lipid composition. These molecular signatures might serve as an early indicator of diabetes induced renal changes, thereby opening up a potential window for treatment before the kidney is damaged beyond repair.