Enriched Eggs Research Articles

Influence of Dietary Zinc Supplementation on Serum Minerals (Zinc, Calcium, Phosphorus) and Liver Enzyme Level (ALP, ALT, GGT) in Layer Chicken

The Indian industry is presently focussed in production of eggs that have higher or enriched levels of certain nutrients. These eggs are capable of safeguarding the health of consumers. Designer eggs have high market demand because of the consumers’ willingness to purchase designer eggs due to its’ nutritional qualities additional to regular eggs. Considering these facts the current study was planned to produce zinc enriched eggs by supplementing laying hens’ diet with required levels of zinc. The present study was conducted at the Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Mannuthy, for 12 weeks. A total of 28 week old thirty two laying hens, belonging to crossbred (White Leghorn N strain and Desi) housed in the animal house attached to the department were used in the experiment. The treatments of this study included a control diet (according to BIS, 2007) fed group and zinc-supplemented group. Each of the experimental treatments had four replicates with four birds with all standard managemental conditions. The functional liver status of birds was not altered due to zinc supplementation, since the present study neither caused any toxicity nor imparted any negative effect on the production performance. Highly significant effect was observed on serum concentration of zinc and calcium. However, the phosphorus levels of treated birds were not significantly different from the control group.

Development of omega-3 enriched egg using fish-oil based fowl feed supplement

Development of omega-3 enriched egg using fish-oil based fowl feed supplement

N-3 PUFA Enriched Eggs as a Source of Valuable Bioactive Substances.

This research elaborates the process of enriching table eggs with n-3 polyunsaturated fatty acids (n-3 PUFA) and presents the effect of such enriched eggs on human health. The experiment was performed on 480 TETRA SL laying hens divided into three groups. Feeding mixtures contained 5% of oils (K = soybean oil, P1 = 3.5% linseed oil + 1.5% fish oil, P2 = 3% linseed oil + 2% fish oil). Referring to the content of α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), eggs of P1 and P2 groups were significantly richer in n-3 PUFA than eggs of the control group (p = 0.001). Atherogenic (AI), thrombogenic (TI), and hypo/hypercholesterolemic (HHI) indexes of egg yolks were more favourable in enriched eggs than in conventional eggs. Fatty acid profiles in the blood of examinees that consumed conventional and enriched eggs (treatments K and P1, respectively) differed significantly in total saturated fatty acids (ΣSFA) (p = 0.041) and in the content of ALA (p = 0.010). The consumption of n-3 PUFA-enriched eggs lowered the Σn-6 PUFA/Σn-3 PUFA ratio in the examinees' blood serum (27%) and had a favourable effect on some blood biochemical indicators. This research confirmed the assumption that the use of a combination of fish and linseed oil in mixtures for laying hens in an amount of up to 5% will increase the content of omega-3 in table eggs, but it was not confirmed that the consumption of these eggs in a short period of time (21 days) has a positive effect on human health.

Patients with Chronic Coronary Syndrome Can Benefit from Consumption of Enriched Chicken Eggs: The Effects on Microvascular Function, Inflammatory Biomarkers, and Oxidative Status—Randomized Clinical Study

The aim of this clinical study was to determine the impact of the consumption of chicken eggs enriched with n-3 polyunsaturated fatty acids, selenium, vitamin E, and lutein on micro- and macrovascular endothelium-dependent dilation, inflammation biomarkers, and oxidative stress levels in participants with chronic coronary syndrome (CCS). This was a double-blind, placebo-controlled clinical study that included 30 CCS participants (9 women, 21 men) randomized into the control group (N = 15), who ate ordinary chicken eggs (three per day), and the Nutri4 group (N = 15), who ate enriched eggs (three per day) for 21 days. Microvascular and macrovascular endothelium-dependent vasodilation was evaluated by measuring forearm skin post-occlusive reactive hyperemia (PORH) and acetylcholine-induced dilation (AChID) and the flow-mediated dilation (FMD) of the brachial artery, respectively. The serum lipid profile, anti- and proinflammatory cytokine levels, serum concentration of nitric oxide synthase (NOS) isoforms, and oxidative stress biomarkers were measured before and after the diet protocols. Enriched, but not regular, chicken eggs significantly improved microvascular PORH and AChID and macrovascular FMD, increased the serum concentration of inducible NOS, decreased serum triglyceride levels, and decreased proinflammatory cytokine IL-17A and TGF-1β levels compared to initial measurements. Patients with CCS can benefit from the consumption of enriched chicken eggs due to improved lipid biomarkers, a more favorable anti-inflammatory milieu, and improved vascular relaxation at micro- and macrovascular levels.

Enhanced Microvascular Adaptation to Acute Physical Stress and Reduced Oxidative Stress in Male Athletes Who Consumed Chicken Eggs Enriched with n-3 Polyunsaturated Fatty Acids and Antioxidants-Randomized Clinical Trial.

This randomized interventional study aimed to determine the effects of n-3 polyunsaturated fatty acids, selenium, vitamin E, and lutein supplementation in the form of enriched chicken egg consumption on microvascular endothelium-dependent vasodilation, oxidative stress, and microvascular response to an acute strenuous training session (ASTS) in competitive athletes. Thirty-one male athletes were assigned to a control (n = 17) or a Nutri4 group (n = 14) who consumed three regular or enriched chicken eggs per day, respectively, for 3 weeks. Significantly enhanced endothelium-dependent responses to vascular occlusion (PORH) and iontophoresis of acetylcholine (AChID) were observed in the Nutri4 group but not in the control group after egg consumption. Formation of peroxynitrite and hydrogen peroxide in peripheral blood mononuclear cells, as well as serum concentration of 8-iso prostaglandin F2α, decreased in the Nutri4 group while remaining unchanged in controls. PORH and AChID were reduced post-ASTS compared with pre-ASTS, both before and after the diets, in both groups. However, the range of PORH responsiveness to ASTS (ΔPORH) increased after consumption of enriched eggs. These results suggest that consumption of enriched chicken eggs has a beneficial effect on microvascular endothelium-dependent vasodilation and the reduction of oxidative stress levels in competitive athletes. Also, microvascular adaptation to the ASTS was improved after consumption of Nutri4 eggs.

In-vitro bio-accessibility and antioxidant activity of commercial standard and enriched whole egg compounds influenced by production and domestic cooking practices

Commercial whole eggs can be enriched to deliver health-beneficial compounds such as lutein and polyunsaturated fatty acids (PUFA). The combined effect of domestic cooking and gastrointestinal (GI) digestion on enriched egg bio-accessibility and biological activity must be fully elucidated. Thus, this study determines how the combined factors modulate whole egg bio-accessibility and antioxidant activity. Eggs from local supermarkets were cooked and subjected to in vitro GI digestion. The eggs and hydrolysates were characterized for their degree of hydrolysis (DH), carotenoid, PUFA, peptide content, and antioxidant activity. The cooking and digestion influence the DH and carotenoids, whereas PUFA and peptide contents remain unaffected. There was no difference in the antioxidant capacity between the different types of eggs. This study introduces a holistic approach to understanding how production and domestic cooking conditions modulate the bio-accessibility and bioactivity of eggs, which could be used to maximize the health benefits of eggs to consumers.

Egg production, egg quality, and fatty acids profiles in eggs and tissues in Lohmann LSL lite hens fed algal oils rich in docosahexaenoic acid (DHA)

Enriching eggs with omega-3 fatty acids (n-3 FA), such as docosahexaenoic acid (DHA), is a well-accepted practice that benefits the egg industry and consumers. However, issues around cost, sustainability, and product acceptance have necessitated the search for alternatives to feeding hens fish oil for DHA enrichment. The effects of feeding 2 algal oils on egg production and DHA enrichment in eggs and selected tissues were investigated. The algal oils were: 1) OmegaPro (OPAO) standardized algal oil for DHA content and 2) Crude algal oil (CAO). A total of 400, 46-wk-old Lohmann LSL lite hens were housed in enriched cages (10 birds/cage) and allocated 5 diets (n=8) for a 12-wk trial. The iso-caloric and -nitrogenous diets were a standard corn and soybean meal diet, standard plus 0.25 or 0.76% OPAO and standard plus 0.23 or 0.69% CAO; algal oils diets supplied similar DHA at each level. Egg production indices (hen day egg production, feed intake, FCR, egg weight, egg mass, and eggshell quality) were monitored for 10 wk. Diet samples were analyzed for fatty acids (FA) on wk 1, 6, and 12 and eggs on wk 4, 5, 6, 9, and 12. At the end of the trial, one hen/cage was weighed and dissected for liver, breast and thigh for FA and long bones for ash content analyses. Concentration of omega-6 to omega-3 FA ratio was 12.9, 6.64, 3.48, 6.96, and 3.59 for standard, 0.23 and 0.76% OPAO, 0.25 and 0.69% CAO, respectively. Algal oils increased (P ≤ 0.046) eggshell thickness linearly. The concentration of DHA in the eggs from the birds fed the standard, 0.23 and 0.76% OPAO, 0.25 and 0.69% CAO was 84, 195, 286, 183, and 297 mg/100g egg, respectively, and algal oils enriched eggs with DHA linearly and quadratically (P ≤ 0.01). In conclusion, algal oils increased the concentration of DHA in eggs and had no adverse effects on egg production and eggshell quality.

The Influence of Storage and Cooking on the Vitamin D Content of 25-Hydroxyvitamin D3-Enriched Eggs

Food fortification is an effective approach to improve vitamin D (VD) concentrations in foods. Eggs are a useful food vehicle for enrichment with VD via its hydroxylated metabolite, 25-hydroxyvitamin D (25-D3), in hen feed. This study determined the impact of time of lay, storage conditions (ambient and refrigeration) and common cooking methods (boiling, frying, scrambling, poaching and microwaving) on the vitamin D metabolite concentration of eggs enriched with 25-D3. Processed samples were freeze-dried and analysed for D3 and 25-D3 using an HPLC-MS(/MS) method. The results indicated that storage and cooking practices influence VD metabolites, with 25-D3 showing true retention of 72–111% and concentrations of 0.67–0.96 µg/100 g of whole egg. Vitamin D3 showed true retention of 50–152% and concentrations of 0.11–0.61 µg/100 g of whole egg. Depending on the storage and method of cooking applied, the calculated total VD activity of enriched eggs ranged from 3.45 to 5.43 µg/100 g of whole egg and was 22–132% higher in comparison to standardised VD content for non-enriched British eggs. The study suggests that 25-D3 is a stable metabolite in eggs following storage and cooking, and that 25-D3-enriched eggs may serve as a potent dietary source of VD.

Exploring Egg Consumption Habits in Surat and Rajkot Cities of Gujarat: A Consumer Behavior Study

The study was conducted to figure out who are the consumers of egg in the concerned area, how much awareness do they have about the enriched egg, what they perceive of an enriched egg in comparison to a conventional egg, to understand the purchasing and consuming behavior of the consumers for an egg and to find out what are the major factors that motivate consumers to purchase enriched egg. The study was conducted in the Surat and Rajkot cities of Gujarat. The objectives of the study were achieved with the help of primary data, which was collected through face-to-face interview using a structured schedule, which was made with the help of pilot survey and review of literatures. Using convenience sampling, 200 households (100 from each city) were surveyed, among which 100 households were enriched egg consumers while the remaining 100 were conventional egg consumers. The majority of the consumers of egg are married male, with education up to graduation, working as a private employee with income in range of ₹35,001 - ₹60,000 but for enriched egg a unanimous consensus on the impact of the socioeconomic factors viz., gender, age, family type and family size, in acceptance of enriched egg, because of the variation in both the geographies can’t be established. The awareness about the enriched egg was less, even among the consumers of enriched egg. The major sources of awareness are friends/relatives, online sources, educational institutions. The date of production, price, size and color highly influence the buying decision whereas health benefit, season and avian disease outbreak highly influence the consumption of egg. The consumers are motivated to purchase enriched egg by the factors in order freshness, health claim, packaging, odorless, golden yellow yolk color and nutritional claim respectively.

Effects of dietary pomegranate seed oil addition to diets for laying hens on fatty acid profile of eggs

In an experiment with 60 laying hens (ISA Brown), 25 weeks of age, effects of dietary pomegranate seed oil supplementation on performance and egg quality were determined. In the 6-week experiment, hens were allocated to five dietary treatments (PSO0, PSO250, PSO500, PSO750 and PSO1000 with pomegranate seed oil addition at an increasing level from 0 to 1000 mg/kg diet respectively). Hens were allocated to 6 cages (replicates)/treatment of 2 hens each and received a diet at 120 g/hen/day.During the experiment, no differences (P > 0.05) occurred among pomegranate seed oil treatments in final body weight (1644 g), feed intake (119.5 g), production of cracked, thin-shelled and shell-less eggs (0.011 eggs/hen/day) and egg weight (61.26 g). However, egg production and egg mass tended to be improved for hens receiving 250 and 500 mg of added pomegranate seed oil in the diet. Feed conversion ratio was tended to be reduced for hens of the PSO250 and PSO500 treatments compared to the other ones. In addition, eggshell weight and yolk index increased, with increasing dietary pomegranate seed oil inclusion levels. The increasing dietary pomegranate seed oil inclusion levels increased egg yolk dry matter content, ash content and antioxidant activity as measured with ferric reducing antioxidant power. Regarding the egg fatty acid profile, the contents of conjugated linoleic acid, saturated fatty acid, polyunsaturated fatty acids were increased. In conclusion, dietary pomegranate seed oil supplementation, at inclusion levels up to 1000 mg/kg of concentrate mixture, tended to improve performance of laying hens and some egg quality characteristics, and increased egg yolk conjugated linoleic acid and polyunsaturated fatty acids content indicating the potential to create enriched eggs.

Natural Multi-Enriched Eggs with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E, and Lutein.

The research investigates the possibilities of enriching eggs with n-3 polyunsaturated fatty acids, selenium, vitamin E, and lutein. The research was carried out on 100 TETRA SL laying hens divided into two groups (C and E). Hens in treatment C (control group) were fed a standard feeding mixture that contained 5% soybean oil, 0.32 mg/kg organic selenium, 25.20 mg/kg vitamin E, and 20.5 mg/kg lutein (Marigold flower extract). Hens in treatment E (experimental group) were fed a mixture in which soybean oil was replaced by 1.5% fish oil + 1.5% rapeseed oil + 2.0% linseed oil. The content of other nutricines amounted to 0.47 mg/kg organic selenium, 125.2 mg/kg vitamin E, and 120.5 mg/kg lutein. Portions of total n-3 polyunsaturated fatty acids in enriched eggs were significantly increased when compared to conventional eggs (480.65:204.58 mg/100 g; p < 0.001). The content of selenium, vitamin E, and lutein was significantly higher (p < 0.001) in enriched eggs than in conventional eggs. The content of selenium in egg albumen was increased by 1.81 times, and in the yolk, it was increased by 1.18 times. At the same time, the content of vitamin E was 2.74 times higher, and lutein was 8.94 times higher in enriched eggs than in conventional eggs.

Vitamin D Fortification of Eggs Alone and in Combination with Milk in Women Aged 44-65 Years: Fortification Model and Economic Evaluation.

For almost nine decades, the fortification of foods with vitamin D has been proven effective in preventing rickets. This study aims to build and economically evaluate a fortification model based on egg biofortification and milk (including yoghurt) fortification. A cross-sectional study was carried out between 1. March and 31. May 2021. Three hundred and nineteen healthy women from the Central Slovenian region aged between 44 and 65 were recruited for the study, with 176 participants included in the final analysis. For the fortification model calculations, the vitamin D contents of unenriched milk (including yoghurt) and eggs were replaced by enriched foods containing vitamin D. The economic evaluation was done using available drug and food supplement prices. Fortification costs were calculated using vitamin D prices provided by suppliers. Mean vitamin D intake from food was 2.19±1.34 µg/d. With fortification Model 1 (enriched eggs), it would be: 6.49±4.45 µg/d, and with Model 2 (enriched eggs and milk): 10.53±6.49 µg/d. Without fortification, none of the participants would reach a daily vitamin D intake >10 µg. With fortification Model 1 (egg fortification), 15.3% would reach >10 µg and with Model 2 (egg and milk fortification) 46.2% would reach >10 µg. The economic comparison of the annual cost of 10 µg vitamin D/d/person was EUR 6.17 for prescription drugs, EUR 6.37 for food supplements, EUR 0.09 for direct milk fortification and EUR 0.12 for egg biofortification with vitamin D. Egg and milk (including yoghurt) fortification could cost-effectively increase vitamin D intake in the Slovenian population of women between 44 and 65 by almost five-fold, and could significantly lower the prevalence of vitamin D deficiency. Additional research and changes to legislation are needed before this can be introduced.

Inclusion of guanidinoacetic acid in the diet of laying hens at late phase of feeding

Context There are few studies on the effects of guanidino acetic acid (GAA) on the performance, quality and fortification of eggs from commercial laying hens. Aims This study aimed to evaluate the effects of dietary supplementation with GAA in the diet of laying hens on productive performance, economic viability and the quality and fortification of eggs. Methods In total, 720 semi-heavy 101-week-old Lohmann LB-Lite® laying hens were distributed in a completely randomised design consisting of five treatments defined by the dietary GAA concentration, with six replicates of 24 birds per experimental unit. Diets were formulated to contain 0.00, 0.05, 0.10, 0.15 or 0.20% GAA. Productive performance and economic viability were evaluated for 12 weeks. Egg quality and fortification were evaluated at 4 and 12 weeks post-GAA inclusion. Key results A negative linear response was found for the bird final weight with an increasing GAA concentration and the optimal value for feed conversion per dozen eggs, as determined by quadratic regression model, was 0.12% GAA. After 4 weeks of GAA inclusion, egg-specific weight responded linearly and positively and yolk colour had a quadratic response, with an optimal concentration at 0.12% GAA. After 12 weeks of GAA inclusion, the optimum level for yolk colour was 0.14% GAA. The treatments did no affect creatine fortification of eggs. Economic analysis showed a linear increase in feed cost with an increasing dietary GAA level. The diet with the best feed cost per kilogram of dozen eggs produced was that with 0.12% GAA inclusion level. Conclusion The inclusion of 0.12% GAA in the diet of commercial laying hens influenced performance parameters and egg quality without fortifying egg creatine concentrations. Implications An egg is a very accessible food and enrichment is a good alternative, as it would help increase consumption of egg nutrients. New research with other products is fundamental for a greater diversification of enriched eggs and better service to consumers.

Topographical distribution of the human brain to Plukenetia volubilis-based omega-3,6,9 enriched egg consumption

<span>Docosahexaenoic acid (DHA) is related to cognitive development. Consumers' brain health could benefit from changes in the omega 6:3 ratio. This study aimed to examine how the </span><em><span>Plukenetia volubilis</span></em><span>-based omega-3,6,9 enriched egg supplementation affects electrical brain activity during the attention/inhibition (Go/NoGo) test. Healthy subjects (n = 20) were chosen at random to eat 2 boiled </span><em><span>Plukenetia volubilis</span></em><span>-based omega-3,6,9 enriched eggs for 12 weeks. The ePrime v.3.0 application recorded behavioral performance during the Go/NoGo test during the electroencephalographic recording. The finding of this study was that twelve weeks of boiled </span><em><span>Plukenetia volubilis</span></em><span>-based omega-3,6,9 enriched egg consumption significantly decreased the reaction time responses compared to the baseline. The topographical distribution revealed that the mean amplitude of N1 produced a slightly larger amplitude in the 12<sup>th</sup> week compared to the baseline. The P3 component, following N1, was also larger in the 12<sup>th</sup> week compared to the baseline. After 12 weeks of consuming the </span><em><span>Plukenetia volubilis</span></em><span>-based omega-3,6,9 enriched eggs, the central nervous system activities during a Go/NoGo test were believed to be enhanced. </span>

Advanced Practical Strategies to Enhance Table Egg Production.

The global demand for table eggs has increased exponentially due to the growing human population. To meet this demand, major advances in hen genetics, nutrition, and husbandry procedures are required. Developing cost-effective and practically applicable strategies to improve egg production and quality is necessary for the development of egg industry worldwide. Consumers have shown a strong desire regarding the improvement of hens' welfare and egg quality. They also become interested in functional and designer foods. Modifications in the nutritional composition of laying hen diets significantly impact egg nutritional composition and quality preservation. According to previous scientific research, enriched egg products can benefit human health. However, producers are facing a serious challenge in optimizing breeding, housing, and dietary strategies to ensure hen health and high product quality. This review discussed several practical strategies to increase egg production, quality, and hens' welfare. These practical strategies can potentially be used in layer farms for sustainable egg production. One of these strategies is the transition from conventional to enriched or cage-free production systems, thereby improving bird behavior and welfare. In addition, widely use of plant/herbal substances as dietary supplements in layers' diets positively impacts hens' physiological, productive, reproductive, and immunological performances.

Egg Quality and Chemical Components of Arabic and Commercial Chicken Enriched with Omega 3 During Storage

The aim of this research was to study the effect of storage periode and egg types on quality and chemical components of DHA enriched egg. One hundred and twenty six each control and DHA enriched eggs used in this research. The research had been implemented using the general linier models procedure included the storage period and eggs treathment and interaction between these factors, comparison test is calculated based on Tukey Test. Internal egg quality was observed, eq,: weight of egg, yolk color and water activity. Internal quality were influenced by egg type. Albumen height and haugh unit value were influenced by both type of chicken and storage periods. Fat and cholesterol levels of egg DHA-rich Arabic and commercial were lower than those of control. However, the percentage of omega-3 fatty acids components were higher in DHA-rich Arabic and commercial than in control eggs.

Lutein and docosahexaenoic acid enriched egg consumption improves retina function in healthy Caucasian older adults

• A full-field electroretinogram was used to assess changes in retina function. • Lutein and DHA enriched eggs significantly increased plasma DHA concentrations. • Lutein and DHA enriched eggs improved rod and cone driven bipolar cell function. • Two eggs daily for six weeks did not adversely impact plasma blood lipid parameters. The purpose of this research was to investigate whether the consumption of lutein and DHA enriched eggs can improve visual function among older adults at risk of age-related eye disease. A total of 30 Caucasian older adults (64.0 ± 3.4 years) consumed enriched eggs (0.87 mg lutein/day, 220 mg DHA/day) daily for 6-weeks. Retina function was assessed by full-field electroretinogram along with plasma DHA, plasma lutein, lipid profiles, and lipoprotein subfractions at day 0 (PRE) and 6 weeks (POST). At POST, maximum amplitude of scotopic a-wave and photopic b-wave significantly increased ( P < 0.001); and plasma DHA levels increased by 35.4% ( P < 0.001). Plasma lipid parameters, lutein and LDL sub-fractions remained unchanged, with a significant increase in large HDL particles and a decrease in intermediate sized HDL particles. Overall, the consumption of two lutein and DHA enriched eggs daily improves electrophysiological retina function without adversely altering plasma lipids in Caucasian older adults.

Astaxanthin improved the storage stability of docosahexaenoic acid-enriched eggs by inhibiting oxidation of non-esterified poly-unsaturated fatty acids

This study assessed the potential and mechanism of action of astaxanthin, to improve the stability of docosahexaenoic acid (22:6(n-3); DHA) enriched egg products, during storage at 4 °C. The reduction in DHA content after 42 days of storage in astaxanthin-DHA eggs (from hens fed supplemental astaxanthin and DHA) was <3%, whereas the reduction in regular-DHA eggs (hens fed DHA only) was over 17%. Astaxanthin also decreased production of oxidation products including 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal and malondialdehyde in eggs during storage, thus markedly improving the oxidative stability of DHA-enriched eggs. The yolk lipidomic profile showed higher intensities for most DHA-containing lipids, especially DHA-phosphatidylcholine, DHA-phosphatidylethanolamine and DHA-non-esterified fatty acid, compared with regular-DHA eggs. Astaxanthin acts primarily by suppressing oxidation of DHA-non-esterified fatty acid, which minimizes the degradation of DHA and appears to be the primary protection mode of yolk DHA during storage.

Cholesterol Content and Fatty Acids Profile in Conventional and Omega-3 Enriched Eggs

This study aimed to produce table eggs enriched with n-3 polyunsaturated fatty acids by diet modification and at the same time, determine the fatty acid profile and cholesterol content in egg yolk, and compare them with that of conventionally produced eggs. Eighty laying hens of Tetra SL hybrids were allocated into two experimental groups. The diets were balanced at the level of 11.60 MJ/kg ME and 16.63% crude protein. The experiment lasted 5 weeks. In the n-3 PUFA eggs, yolk cholesterol levels were 1179.25 mg/100 g compared to the 1287.46 mg/100 g measured in the control eggs. The difference of 108.21 mg/100 g i.e. 8.4% was not statistically significant (p>0.05). The fatty acid profile in n-3 PUFA versus control eggs was as follows: SFA 30.55 vs 35.89% (p<0.001), MUFA 42.61 vs 42.28%, n-6 PUFA 22.10 vs 21.40% (p>0.05), and n-3 PUFA 4.74 vs 0.82% (p<0.001). Furthermore, ALA content was 2.28 vs 0.26%, EPA 0.31 vs 0.14%, and DHA 2.20 vs 0.43% (p<0.001) in n-3 PUFA vs control eggs, respectively. The n-6/n-3 PUFA ratio in the enriched eggs was 5.11 and in the conventional ones 23.73 (p<0.001). Based on the results of our study, it can be concluded that in n-3 PUFA-enriched eggs, the n-6/n-3 PUFA ratio is significantly improved compared to the conventional eggs. Thus, n-3 PUFA eggs are nutritionally and functionally more favorable than conventional eggs for human nutrition.

Halal production of omega 3 enriched eggs on the farm Pećigrad, B&amp;H

The aim of this research is to develop a functional food and halal Omega 3 eggs by dietary manipulation of laying hens as well as to investigate the influence of nutrient composition on egg yolk content of PUFAs. The research was conducted on the farm Pećigrad, Velika Kladuša, which has 21,162 laying hens, with an egg production rate of 70%. Laying hens were fed with halal and NON GMO feed mixtures from three suppliers. Omega 3 eggs contained 318 mg of Omega 3 PUFAs, which is 6 times more than the standard eggs. The n-6:n-3 PUFAs ratio was 18.4:1.9=9.9 in egg yolks of laying hens fed with standard feed mixture, while in egg yolks produced by laying hens fed with feed mixture enriched with flaxseed this ratio was 17.8:5.3=3.4. Consumption of eggs with the latter n-6:n-3 ratio can prevent cardiovascular diseases and provides the intake of RDA of Omega 3 FAs. Traceability and established HrCCP in the chain of suppliers, in processes of primary and industrial production, application of good practices, sustainable development resulted in halal products with additional functional properties.