Abstract Three Typha species were micropropagated successfully using immature inflorescence segments as the explant. Plantlet production via organogenesis was optimum when the explants were first placed on LS medium + 5.0 mg°liter−1 2,4-D to initiate callus production, and after 9½ weeks the callus was recultured on LS + 1.0 mg°liter−1 benzyladenine (BA). Of three species tested, T. glauca produced callus and new shoots more readily than T. latifolia or T. angustifolia. As inflorescences matured, an increased level of an auxin-like plant growth regulator, picloram, was necessary for callus induction. Excision and separate culture of green “spots” or clumps that formed on the calli enhanced shoot regeneration from the callus. Chemical names used: (2,4-dichorophenoxy)acetic acid (2,4-D) and A-(phenylmethyl)-1H-purin-6-amine (BA).