Enhanced Efficiency Fertilizers Research Articles

Long-Term Effects of Nitrogen Sources on Yields, Nitrogen Use Efficiencies, and Soil of Tilled and Irrigated Corn

Although corn is the most important and nitrogen (N)-fertilized crop, there is a lack of long-term data on the effects of organic and inorganic N fertilizers on the N balance and losses for corn systems under different tillage approaches. From 2012 to 2023, we assessed the effects of the N source on the grain yields from cultivated continuous corn receiving irrigation at a site with minimal erosion in Fort Collins, Colorado, USA, and compared these effects to no-till (NT) and strip till (ST) systems receiving inorganic N. An N balance accounting for N and carbon (C) sequestration found a system nitrogen use efficiency (NUESys) for organic N fertilizer (manure) with a tillage of 86.6%, which was higher than the NUESys of 62.6% with inorganic N fertilizer (enhanced efficiency fertilizer, EEF). Conventional tillage with manure use is a good management practice that contributed to higher grain yields (2 of 11 years), C sequestration (p < 0.05), soil organic N content (p < 0.05), and soil phosphorus (P) content than inorganic N fertilizer with tillage (p < 0.05). The tilled systems, whether receiving organic or inorganic N fertilizer, had higher yields and grain N content than the NT and ST systems receiving inorganic N fertilizer (p < 0.05). The grain production of the cultivated system receiving organic N fertilizer did not decrease with time, while the yields of the cultivated system receiving inorganic N fertilizer decreased with time (p < 0.05), suggesting that cultivated systems receiving organic N fertilizer may be more sustainable and better able to adapt to a changing climate. Additionally, a combination of manure (30% of N input) with EEF (70% of N input) contributed to a synergistic effect that increased the agronomic productivity (harvested grain yields).

Enhanced efficiency fertilizer: a review on technologies, perspectives, and research strategies

Enhanced efficiency fertilizer: a review on technologies, perspectives, and research strategies

Field Evaluation of Urea Fertilizers Enhanced by Biological Inhibitors or Dual Coating

Relative to soluble N sources, enhanced-efficiency fertilizers (EEFs) support steady turfgrass growth and dense canopy quality while abating N loss as nitrate, ammonia, and/or N2O from turfgrass systems. Modern EEFs provide turfgrass managers greater operational effect and versatility in their nutrient management efforts and compel field characterization of their temporal response. Likewise, field confirmation of commercial EEF nutrient recovery helps stakeholders select the appropriate EEF for their specific application. Our research objective was to quantify the temporal response of Kentucky bluegrass growth/yield, canopy density and color, and fertilizer N recovery to a practical application of conventional urea or an enhanced-efficiency granular fertilizer. In May 2014 and June 2018, Kentucky bluegrass plots were fertilized by granules of conventional urea, N-(n-butyl) thiophosphoric triamide (NBPT)-, and dicyandiamide (DCD)-stabilized urea, or polymer-/sulfur-coated urea (PSCU) at a N rate of 43.9 kg ha−1 (0.9 lbs/1000 sq. ft.). The dependent variable response over the two growing seasons was highly affected by efficiency enhancement. Following the repeated 16.5-week evaluations, the mean percent of fertilizer N recovered from conventional urea, stabilized urea, and PSCU totaled 57.5, 68.4, and 89.1%, respectively. In the 23 to 51 days from treatment (DFT), recovery of PSCU-N significantly exceeded that from conventional or stabilized urea.

Synthesis and anti-ureolitic activity of Biginelli adducts derived from formylphenyl boronic acids

Urease is a metalloenzyme that contains two Ni(II) ions in its active site and catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The development of effective urease inhibitors is crucial not only for mitigating nitrogen losses in agriculture but also for offering an alternative treatment against infections caused by resistant pathogens that utilize urease as a virulence factor. This study focuses on synthesizing and investigating the urease inhibition potential of Biginelli Adducts bearing a boric acid group. An unsubstituted or hydroxy-substituted boronic group in the Biginelli adducts structure enhances the urease inhibitory activity. Biophysical and kinetics studies revealed that the best Biginelli adduct (4e; IC50 = 132 ± 12 µmol/L) is a mixed inhibitor with higher affinity to the urease active site over an allosteric one. Docking studies confirm the interactions of 4e with residues essential for urease activity and demonstrate its potential to coordinate with the nickel atoms through the oxygen atoms of carbonyl or boronic acid groups. Overall, the Biginelli adduct 4e shows great potential as an additive for developing enhanced efficiency fertilizers and/or for medical applications.

Changing the Sugarcane N Credit and C Fluxes in Soil by the Application of N-Enhanced Efficiency Fertilizers

The accumulation of sugarcane residues (ASR) on the soil surface in areas with mechanized harvest increases the soil quality with a direct effect on crop production. However, the ASR presents a high C:N (carbon: nitrogen) which promotes N immobilization. The application of N enhanced efficiency fertilizers (EFF) could be an alternative to increase the sugarcane N credit and C in the soil due to inputs in soil. Our goal here was to monitor the additions of EFF in soil and the influence in routes of C stabilization and N credit. A laboratory study was run with the association of urea/U, urea + sulfur/U + S, urea + copper + boron/U + CuB, or urea-kimcoat/U + Kc with sugarcane residue in the soil. Soil C (total organic carbon/TOC, humin/HU, humic acid/HA, and fulvic acid/FA), N (soil and residues) and CO2 emissions were monitored. Results showed that the application of U and U + CuB presented a higher CO2 emission, which created a negative balance in TOC and FA. The routes of C were increased in HA, while there was a decrease in FA. The credit N of fertilizers and sugarcane residue were positive, with an average of 90% and 86%, respectively. Using machine learning, the U + S and U + Kc presented a lower CO2 emission, if compared with the performance of U and U + CuB. Based on these results we conclude that the U and U + CuB presents a lower performance with higher C losses with reduction of carbon in soil.

Development and characterization of enhanced urea through micronutrients and established technology addition

AbstractIt is necessary to increase the agronomic use efficiency of urea to reduce ammonia volatilization and increase crop yield. However, relying solely on urea for the enhanced efficiency technologies development could harm fertilizer integrity, resulting in reduced application quality and fertilizer storage time. The authors aimed at developing and characterizing the physical, chemical, and physicochemical quality of a novel enhanced efficiency fertilizers, synthesized from urea plus boron (B), zinc (Zn), nickel (Ni), or molybdenum (Mo) addition, with or without N‐(n‐butyl) thiophosphoric triamide (NBPT) associated. Hygroscopicity, hardness, salt index (SI), pH, and thermogravimetric decomposition were the parameters evaluated. Fertilizer quality was assessed through microscopic X‐ray fluorescence, microtomography, and scanning electron microscopy. Micronutrients were added by coating or granulation; they were homogeneously distributed over the fertilizer's granules. The fertilizers’ hardness increased up to 86% with Zn coated compared with uncoated. Granulated urea with B, Zn, Ni, or Mo had greater internal porosity, which resulted in lower hardness. Boron and Zn addition to the fertilizers increased the hygroscopicity in average 388% and 473%, respectively, compared with hygroscopicity observed for urea. Moreover, hygroscopicity was increased by an average of 56% with NBPT addition. Micronutrients addition to the urea granules increased the SI, while thermal decomposition stages of urea were unaffected by micronutrients addition. Enhanced efficiency fertilizers require characterization before agronomic efficiency tests due to changes in their physical, chemical, and physicochemical properties. Unfavorable changes could harm granules integrity and application efficiency in the field, resulting in economic losses to the industry and farmers.

Nitrogen: from discovery, plant assimilation, sustainable usage to current enhanced efficiency fertilizers technologies – A review

ABSTRACT From 1913 onwards, the global situation changed from a scenario of nitrogen (N) scarcity to an abundance of ammonia (NH 3 ) produced synthetically via the Haber-Bosch process. Several N compounds have been synthesized since then, with urea becoming the main source of N, accounting for 55 % of current N consumption. However, N efficiency in agroecosystems is low and, normally, N recovery in cultivated plants is less than 50 %. This occurs because a large amount of reactive N is lost to the environment, inducing various forms of pollution, threatening human and environmental health, in addition to causing a negative economic impact on the farmer. The main processes responsible for low N efficiency are NH 3 volatilization, leaching, and N denitrification. Considering global NH 3 volatilization losses of 14 %, it can be assumed that up to 8.6 million Mg of urea are lost every year in the form of NH 3 . For each ton of NH 3 produced, 1.9 to 3.8 Mg of CO 2 is emitted into the atmosphere. Therefore, increasing N use efficiency (NUE) without compromising yield is a necessity and a challenge for crop improvement programs and current management systems, in addition to reducing greenhouse gas emissions. In this context, enhanced efficiency fertilizers (EEFs), which contain technologies that minimize the potential for nutrient losses compared to conventional sources, are an alternative to increasing the efficiency of nitrogen fertilization. Currently, EEFs are classified into three categories: stabilized, slow-release, and controlled-release. This study aims to understand the technologies used to produce EEFs and the factors that govern their availability to plants. This review covers the following topics: the discovery of N, N dynamics in the soil-atmosphere system, N assimilation in plants, strategies to increase NUE in agrosystems, NH 3 synthesis, NH 3 volatilization losses, N fertilizer technologies, the importance of characterization of EEFs, conventional nitrate or ammonium-based fertilizers to reduce gaseous losses of NH 3 and future prospects for the use of N fertilizers in agriculture.

Global impact of enhanced-efficiency fertilizers on vegetable productivity and reactive nitrogen losses

Vegetables are the most consumed non-staple food globally, and their production is crucial for dietary diversity and public health. Use of enhanced-efficiency fertilizers (EEFs) in vegetable production could improve vegetable yield and quality while reducing reactive nitrogen (Nr) losses. However, different management and environmental factors has significantly distinctive impacts on the effectiveness of EEFs. In this study, a worldwide meta-analysis based on the data collected from 144 studies was performed to assess the impacts of EEF (nitrification inhibitor [NI] and polymer-coated urea [PCU]) application on vegetable yield, nitrogen (N) uptake, nitrogen use efficiency (NUE), vegetable quality and Nr losses (nitrous oxide [N2O] emissions, ammonia [NH3] volatilization, and nitrate [NO3−] leaching). The effects of the applied EEFs on vegetable yields and N2O emissions were assessed with different management practices (cultivation system, vegetable type and N application rate) and environmental conditions (climatic conditions and soil properties). Compared to conventional fertilizers, EEFs significantly improved vegetable yield (7.5–8.1 %) and quality (vitamin C increased by 10.7–13.6 %, soluble sugar increased by 9.3–10.9 %, and nitrate content reduced by 17.2–25.1 %). Meanwhile, the application of EEFs demonstrated a great potential for Nr loss reduction (N2O emissions reduced by 40.5 %, NO3− leaching reduced by 45.8 %) without compromising vegetable yield. The NI was most effective in reducing N2O emissions (40.5 %), but it significantly increased NH3 volatilization (32.4 %). While PCU not only significantly reduced N2O emissions (24.4 %) and NO3− leaching (28.7 %), but also significantly reduced NH3 volatilization (74.5 %). And N application rate, soil pH, and soil organic carbon (SOC) were the main factors affecting the yield and environmental effects of EEFs. Moreover, the yield-enhancing effect of NI and PCU were better at low soil N availability and SOC, respectively. Thus, it is important to adopt the appropriate EEF application strategy targeting specific environmental conditions and implement it at the optimal N application rate.

Leveraging digital agriculture for on-farm testing of technologies

The Precision Nitrogen Project (PNP) worked with more than 80 corn and winter wheat producers to inexpensively design and implement randomized, replicated field strip trials on whole commercial farm fields, and to provide site-specific testing of current nitrogen (N) technologies. This article proposes a conceptual framework and detailed procedure to select the N technology to be tested; design and implement field trials; generate, process, and manage field trial data; and automatically analyze, report, and share benefits from precision N technology. The selection of the N technology was farmer-driven to ensure a good fit and to increase the likelihood of future technology adoption. The technology selection method was called the “N tiered approach”, which consisted of selecting a technology that progressively increases the level of complexity without exceeding the farmer’s learning process or farm logistic constraints. The N tools were classified into (1) crop model-based, (2) remote sensing-based, (3) enhanced efficiency fertilizers, and (4) biologicals. Field strip trials comparing producers’ traditional management and the selected N technology were combined with site-specific N rate blocks placed in contrasting areas of the fields. Yield data from the N rate blocks was utilized to derive the site-specific optimal N rate. The benefits of current N technologies were quantified by comparing their yield, profit, and N use efficiency (NUE) to growers’ traditional management and to the estimated site-specific optimal N rate. Communication of the trial results back to the growers was crucial to ensure the promotion and adoption of these N technologies farm wide. The framework and overall benefits from N technologies was presented and discussed. The proposed framework allowed researchers, agronomists, and farmers to carry out on-farm precision N experimentation using novel technologies to quantify benefits of digital ag technology and promote adoption.

Evaluating novel biodegradable polymer matrix fertilizers for nitrogen-efficient agriculture.

Enhanced efficiency fertilizers (EEFs) can reduce nitrogen (N) losses in temperate agriculture but are less effective in the tropics. We aimed to design a new EEF and evaluate their performance in simple-to-complex tests with tropical soils and crops. We melt-extruded urea at different loadings into biodegradable polymer matrix composites using biodegradable polyhydroxyalkanoate (PHA) or polybutylene adipate-co-terephthalate (PBAT) polymers with urea distributed throughout the pellet. These contrast with commercially coated EEF that have a polymer-coated urea core. We hypothesized that matrix fertilizers would have an intermediate N release rate compared to fast release from urea or slow release from coated EEF. Nitrogen release rates in water and sand-soil columns confirmed that the matrix fertilizer formulations had a more progressive N release than a coated EEF. A more complex picture emerged from testing sorghum [Sorghum bicolor (L.) Moench] grown to maturity in large soil pots, as the different formulations resulted in minor differences in plant N accumulation and grain production. This confirms the need to consider soil interactions, microbial processes, crop physiology, and phenology for evaluating fertilizer performance. Promisingly, crop δ15N signatures emerged as an integrated measure of efficacy, tracking likely N conversions and losses. The three complementary evaluations combine the advantages of standardized high-throughput screening and more resource-intensive and realistic testing in a plant-soil system. We conclude that melt-blended biodegradable polymer matrix fertilizers show promise as EEF because they can be designed toward more abiotically or more microbially driven N release by selecting biopolymer type and N loading rate.

Novel core-shell microcapsules incorporating macro/micronutrients in PVA/starch matrix

Enhanced efficiency fertilizers (EEFs) have been widely used due to their ability to meet crop nutritional needs and reduce the main problem of leaching. In this work, we compared the effects of spray-dryer microspheres and microcapsules on nutrient encapsulation and identified the spheres and core-shell structures by fluorescence microscopy using Rhodamine B (RB) as the fluorescent material. The microsphere matrix was based on starch, and the microcapsules consisted of starch as the core and poly (vinyl alcohol) as the shell. The fluorescent images confirmed that the RB was incorporated into the starch, as the overlap of fluorescence and brightfield images covered the entire area for microspheres and only the core for microcapsules. Microspheres exhibited an irregular and non-spherical morphology, whereas microcapsules presented a spherical shape with a smooth and homogeneous surface featuring small aggregated particles. The microparticle structures remained unchanged even after incorporating KNO3, Fe, Cu, and Mn. Thermal analysis indicated lower initial mass loss temperatures for the microcapsules, suggesting polymer interactions during drying and formation. FTIR further supported this, which revealed structural changes and partial oxidation during drying, contributing to reduced thermodegradation. Notably, micronutrient-based microcapsules demonstrated a significantly lower burst release than macro/micronutrient-based microcapsules and micronutrient-based microspheres. Fe and Cu were released most rapidly from microspheres in the first hour, reaching 84.2% and 79.4%, respectively. In contrast, macro/micronutrient-based microcapsules released 66.1% and 67.7%, while micronutrient-based microcapsules released 53.1% and 61.5%. As for Mn, macro/micronutrient-based microcapsules exhibited the highest initial release at 72.4%, followed by micronutrient-based microcapsules at 62.2%, with microspheres releasing at 55.8%. Additionally, the Peppas-Sahlin model provided the best fit, indicating diffusional and polymer relaxation contributions. The morphology, release, and kinetics behaviors confirmed that the core-shell prolonged the nutrient release by reducing the initial burst release, thanks to the diffusion barrier provided by the PVA layer.

Effects of straw mulching, liming, and soil texture on ammonia volatilisation: a study of conventional and enhanced efficiency fertilisers

Context In no-tillage agriculture, maintenance of soil cover combined with liming without incorporation increases nitrogen (N) loss via ammonia (NH3) volatilisation, decreasing the efficiency of nitrogen fertilisers. Aims To quantify N losses by NH3 volatilisation from conventional and enhanced efficiency fertilisers applied to a clayey and a sandy loam soil subjected or not to lime (CaCO3) application and straw mulching. Methods Two laboratory experiments were carried out; one using a clayey soil, and the other using a sandy loam soil. Both experiments followed a 4 × 2 × 2 factorial design with four N sources (urea, urea-NBPT, urea-formaldehyde, and ammonium sulfate), absence and presence of liming, and absence and presence of Brachiaria ruziziensis straw mulching. NH3 volatilisation was measured using closed flasks containing filter paper soaked with sulfuric acid and quantified by titration with sodium hydroxide. Key results NH3 volatilisation was up to 62% of the N applied. Losses due to NH3 volatilisation from both soil types decreased in the following order of treatment: liming + straw mulching > straw mulching only > liming only. Urea-formaldehyde and ammonium sulfate were the most efficient in reducing NH3 emissions. However, when ammonium sulfate was applied to a clayey soil after liming, it resulted in higher NH3 emissions than conventional urea. Conclusions Urea-formaldehyde showed better performance in reducing NH3 losses due to greater stability in the presence of straw or liming. Implications Soils with straw and limestone can lead to large NH3 volatilisation losses if urea conventional is broadcast.

Modifying soluble NPK release with hydrophobized nanocellulose-based hydrogels for sustainable enhanced efficiency fertilizers

Introduction of a hydrophobic shell to a regenerated cellulose-based bead through gas-phase esterification results in slowing of plant nutrient release by an order of magnitude.

Integrating Enhanced‐Efficiency Fertilizers in 4R Nutrient Management

AbstractThe 4R nutrient stewardship framework has brought us closer to improving our nutrient use efficiency. However, given the inherent “leakiness” of the N cycle, it is challenging to increase N use efficiency to optimum levels. Newer fertilizer technologies, like enhanced‐efficiency fertilizers (EEFs), are tools that can help achieve this goal. To determine if EEFs should be incorporated into nutrient management programs, growers and crop advisers should consider the various types of EEFs available, their functions within the soil, and the timing of application relative to the crop’s nutrient uptake demand. Earn 1 CEU in Nutrient Management by reading this article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.

Enhanced-efficiency fertilizers on the growth of coffee plants under weed (Bidens Pilosa) competition stress

The interference of weeds in coffee cultivation is one of the factors contributing to losses and impairments in crop growth and productivity. Consequently, it becomes necessary to employ strategies aimed at mitigating this stress and facilitating optimal crop development through enhanced nutritional practices. This study aimed to assess the impact of various sources and modes of release of enhanced-efficiency phosphate fertilizers on the initial growth of Coffea arabica L. plants in competition with the weed Bidens pilosa in a greenhouse. The experiment was conducted at the Federal University of Vales do Jequitinhonha and Mucuri – Diamantina MG, Brazil, employing a block design in a 4 x 6 factorial arrangement with four replications. The factors corresponded to four levels of B. pilosa infestation and six phosphorus sources (Super Single, Conventional, MAP, Granulated Organomineral, Pelleted Organomineral, MAP Coated with Polymer, and a control treatment without fertilization). The findings suggest that under the experimental conditions of this research, the escalating density of weed competition from B. pilosa negatively impacts the initial growth of coffee plants. However, this effect is dependent on the source of phosphate fertilization. Fertilizers with slow-release phosphate sources are recommended for coffee plantations, as they contribute to reduced weed growth in comparison to coffee plants.

Mitigate N2O emissions while maintaining sugarcane yield using enhanced efficiency fertilisers and reduced nitrogen rates

Mitigate N2O emissions while maintaining sugarcane yield using enhanced efficiency fertilisers and reduced nitrogen rates

Improving Nitrogen Use Efficiency for Corn (Zea mays L.) Production

Nitrogen (N) fertilizer is one of the main expenses for crops in high-input agricultural systems. Insufficient use of N is not only responsible for higher cost of crop production, but also for environmental pollution. Corn is one of the most important crop in the world. Application of N is a primary method used to maintain soil nutrients and increase corn yield. economic and environmental challenges are driving increased interest in improving NUE. Opportunities abound for improving NUE through implementation of fundamental BMPs, including selection of genetics with high NUE, development of enhanced-efficiency fertilizer products (CRF and NI), precision nutrient application at optimum rate, timing and placement, and carefully water management.

Integrating enhanced efficiency fertilizers and nitrogen rates to improve Canada Western Red Spring wheat

Granular urea fertilizer applied at planting is prone to nitrogen (N) losses in certain environments. Enhanced efficiency fertilizers (EEFs) are developed to mitigate losses and optimize plant uptake. To determine the benefits of EEFs in grain yield and quality enhancement in Canada Western Red Spring (CWRS) wheat, an experiment was conducted from 2019 to 2022 at eight sites in Alberta and Saskatchewan, Canada. The effects of five N sources [urea; urea + urease inhibitor, N-( n-butyl)thiophosphoric triamide (NBPT); urea + nitrification inhibitor, nitrapyrin; urea + dual-inhibitor, NBPT + dicyandiamide; and polymer-coated urea, ESN® (Environmentally Smart Nitrogen®)] and four N rates (60, 120, 180, and 240 kg N ha−1) on CWRS wheat production were examined. Results indicated that N source affected grain yield in Dark Brown Chernozem soils but not in Black Chernozem or Dark Grey Luvisol soils. In Dark Brown Chernozem soils, a dual inhibitor increased grain yield by 3.1% and 3.9% relative to urea and polymer-coated urea, respectively, while all other EEFs attained similar results. The use of a dual inhibitor EEF led to greater net returns compared to urea and polymer-coated urea in the Dark Brown Chernozem soils. Grain protein concentration increased linearly with increasing N rate from 60 to 240 kg N ha−1. Generally, a rate of 120 kg N ha−1 was optimal for CWRS wheat grown in Canadian prairie conditions when coupled with EEFs, particularly a dual inhibitor, and grain yield and protein were often responsive.

Enhanced efficiency urea fertilizers and timing effects on N2O emissions from spring wheat production in Manitoba

AbstractOpportunities exist to reduce nitrous oxide (N2O) emissions from nitrogen (N) fertilizers using enhanced efficiency fertilizers (EEFs) and managing application timing. This study examined (1) application timing (fall/spring) and (2) fertilizer N source on N2O emissions, yield, and N uptake of Canadian hard red spring wheat (Triticum aestivum L.) in Southern Manitoba. Fertilizer N sources included granular urea and four EEF products: (1) polymer‐coated urea (environmentally smart nitrogen [ESN]); (2) urea plus nitrification inhibitor (eNtrench); (3) urea plus urease inhibitor (Limus); and (4) urea plus nitrification and urease inhibitor (SuperU). Nitrification‐inhibited products most consistently reduced N2O emissions while maintaining productivity. Compared to urea alone, urea + eNtrench was most effective in reducing cumulative N2O emissions by 47%–64% at four of six site‐years. SuperU reduced N2O emissions by 37%–57% at three of six site‐years. ESN and urea + Limus did not affect emissions in most years. Wheat yield, protein, and N uptake were unaffected by N source in five of six site‐years. Compared to spring, fall application gave greater N2O emissions by 33%–67% at three of six site‐years due to spring‐thaw emissions. Fall was inferior to spring application in wetter site years with lower yield, protein, and N uptake. Overall, nitrification‐inhibited products—either alone or with a urease inhibitor—are a promising tool to reduce N2O emissions while maintaining wheat productivity in Manitoba. However, given that there were few consistent increases in yield or protein, the additional cost of the inhibitors will be a barrier to adoption.

Drug discovery-based approach identifies new nitrification inhibitors

Nitrogen (N) fertilization is crucial to sustain global food security, but fertilizer N production is energy-demanding and subsequent environmental N losses contribute to biodiversity loss and climate change. N losses can be mitigated be interfering with microbial nitrification, and therefore the use of nitrification inhibitors in enhanced efficiency fertilizers (EEFs) is an important N management strategy to increase N use efficiency and reduce N pollution. However, currently applied nitrification inhibitors have limitations and do not target all nitrifying microorganisms. Here, to identify broad-spectrum nitrification inhibitors, we adopted a drug discovery-based approach and screened 45,400 small molecules on different groups of nitrifying microorganisms. Although a high number of potential nitrification inhibitors were identified, none of them targeted all nitrifier groups. Moreover, a high number of new nitrification inhibitors were shown to be highly effective in culture but did not reduce ammonia consumption in soil. One archaea-targeting inhibitor was not only effective in soil, but even reduced - when co-applied with a bacteria-targeting inhibitor - ammonium consumption and greenhouse gas emissions beyond what is achieved with currently applied nitrification inhibitors. This advocates for combining different types of nitrification inhibitors in EEFs to optimize N management practices and make agriculture more sustainable.