English Setter Research Articles

Long-term dietary intervention of the hydrolyzed feather meal on microbiota composition of adult female dogs

This research delves into the impact of substitution of 7% of poultry meal (PM) with hydrolyzed feather meal (HFM) on the animal performance and fecal microbiota of dogs. The study was conducted on six adult female English setter dogs, split into control (CTR), and treated (TRT) dietary treatment groups, with similarity for age, initial body weight, and body condition score. During 45-days, body weight, body condition score, muscle condition score, fecal consistency score and microbiota were monitored at the beginning of the trial and after 3, 7, 15 and 45 days. No significant differences (P > 0.05) were observed for these parameters over the whole period of the trial, neither in relation to diet, nor to time, nor to the interaction diet x time. Significant differences were observed for alpha between diets and the TRT group displayed higher evenness compared to the CTR group. Beta diversity analysis revealed distinctions in microbiota composition between the CTR and TRT groups, with the former demonstrating higher biodiversity. Discriminant analysis highlighted 9 significant taxa and 6 of them were significantly different at the Kruskal Wallis test between diets. The results showed an increase in relative abundance (RA) for the CTR group of Streptococcus, Colinsella stercoris, Ruminococcus gnavus, and Bacteroides coprophilus. Conversely, higher RA was observed in the TRT group for Peptostreptococcaceae and Bacteroides uniformis. These findings indicated that the inclusion of hydrolyzed feather meal in the diet of dogs is well accepted and do not have adverse effects in the parameters analyzed.

Exploring the impact of epidemiological and clinical factors on the progression of canine leishmaniosis by statistical and whole genome analyses: from breed predisposition to comorbidities

Canine leishmaniosis (CanL), caused by Leishmania infantum, is a complex disease of growing importance in Europe. Clinical manifestations result from the down-modulation of the host immune response through multiple host-parasite interactions. Although several factors might influence CanL progression, this is the first known study evaluating risk factors for its different clinical stages in a large referral hospital population (n = 35.669) from an endemic area, over a 20 year period. Genome-wide scans for selection signatures were also conducted to explore the genomic component of clinical susceptibility to L. infantum infection. The prevalence of CanL was 3.2% (16.7% stage I; 43.6% stage II; 32.1% stage III; 7.6% stage IV). Dog breed (crossbreed), bodyweight (<10 kg), living conditions (indoors), regular deworming treatment, and being vaccinated against Leishmania significantly decreased the transmission risk and the risk for developing severe clinical forms. Conversely, the detection of comorbidities was associated with advanced clinical forms, particularly chronic kidney disease, neoplasia, cryptorchidism, infectious tracheobronchitis and urate urolithiasis, although those did not impact the clinical outcome. Significant associations between an increased risk of severe clinical stages and findings in the anamnesis (renal or skin-related manifestations) and physical examination (ocular findings) were also detected, highlighting their diagnostic value in referred cases of CanL. Sixteen breeds were found to be significantly more susceptible to developing severe stages of leishmaniosis (e.g. Great Dane, Rottweiler, English Springer Spaniel, Boxer, American Staffordshire Terrier, Golden Retriever), while 20 breeds displayed a clinical resistantance phenotype and, thus, are more likely to mount an efficient immune response against L. infantum (e.g. Pointer, Samoyed, Spanish Mastiff, Spanish Greyhound, English Setter, Siberian Husky). Genomic analyses of these breeds retrieved 12 regions under selection, 63 candidate genes and pinpointed multiple biological pathways such as the IRE1 branch of the unfolded protein response, which could play a critical role in clinical susceptibility to L. infantum infection.

Florfenicol urinary excretion and its potential for treating canine urinary tract infections.

The canine urinary excretion of florfenicol was evaluated to explore its potential for treating urinary tract infections. Nine healthy male intact purpose-bred Beagles and four healthy client-owned dogs each received a single oral dose of florfenicol 20 mg/kg (300 mg/mL parenteral solution) with food. All voluntary urinations were collected for 12 h. Although florfenicol is reportedly bitter tasting, 7/9 Beagles and 4/4 client-owned dogs completely ingested the florfenicol and were enrolled; salivation (n = 1) and headshaking (n = 3) were observed. The last measured urine florfenicol concentrations were variable: Beagles (0.23-3.19 mcg/mL), Pug (3.01 mcg/mL) English Setter (21.29 mcg/mL), Greyhound (32.68 mcg/mL), and Standard Poodle (13.00 mcg/mL). Urine half-life was similar for the Beagles and the Pug, 0.75-1.39 h, whereas the half-life was 1.70-1.82 h for the English Setter, Greyhound, and Standard Poodle. Larger breed dogs exceeded 8 mcg/mL florfenicol (wild-type cutoff) in their urine at 12 h, whereas the Beagles and Pug had <8 mcg/mL; it is unclear if this is an individual, breed, or size difference. These data suggest oral florfenicol may need to be administered q6-12h for canine urinary tract infections, but further data are needed (more enrolled dogs, multiple-dose regimens) before considering clinical trials or breed-specific differences.

Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters

This study aims to determine the morphological differences according to gender via radiographic images taken from the carpal bones of English setters. A total of 28 bone samples, 16 females and 12 males, were used in the study. The shape differences between males and females were evaluated by placing 27 landmarks on the X-ray images. Principal Component Analyses are used to measure the distribution of variance. In addition, these analyses take the mean shape variation and compare it to shape differences. As a result of this analysis, the value of PC1 explained 24.34% of the total variation. In addition, PC2 explained 16.24% of the total variation, and PC3 explained 10.45%. In addition, the results of PCA were also described in terms of the English setter samples used. Then, Discriminant Function analysis was performed using the same software program. With this analysis, it was tried to determine the differences between the genders. According to the discriminant function test, 13 of 16 female dogs were completely separated from male dogs. Also, all 12 male dogs were separated from female dogs according to the same test results. In short, because of this study, shape differences in the carpal bones of English setters were investigated according to gender.

Breed distributions for diabetes mellitus and hypothyroidism in Norwegian dogs

BackgroundDiabetes mellitus (DM) and hypothyroidism are common canine endocrinopathies. Both canine DM and primary hypothyroidism are assumed to originate from autoimmune destruction of the respective endocrine glands and have been associated with the major histocompatibility complex (MHC) gene region. This study aims to investigate breed distributions for DM and hypothyroidism in the Norwegian canine population by calculating odds ratios (OR) from two different comparator groups.MethodsResults from canine serum samples submitted from 2001 to 2018 to the Veterinary Clinical Pathology Laboratory (VCPL) at the Faculty of Veterinary Medicine, Norwegian University of Life Sciences for analysis of fructosamine and thyroid hormones in serum were used as cases in a retrospective bivariate analysis of canine breeds. The ORs were calculated as a measure of risk for the included breeds, where all the submitted blood samples to the VCPL and dogs registered in the Norwegian Kennel Club (NKK), the national organization for dog owners, were used as two comparator groups.ResultsSignificant differences in disease prevalence between breeds were discovered using both comparator groups. Australian terrier, Swedish lapphund, Samoyed, and Schipperke were at highest risk for DM. German Shepherd, Golden retriever, German pointing dog, and Collie presented as the breeds with lowest risk for DM. For hypothyroidism, Schnauzer, Eurasier, Dunker, and English setter were at highest risk for developing the disease. The breeds at lowest risk of developing hypothyroidism were Rottweiler, Dachshund, German shepherd, and Border collie. The results from the different comparator groups gave different ORs and ranks, but the breeds with highest and lowest odds showed the same susceptibility using both comparators.ConclusionsThese findings support that there are breeds more and less prone to develop DM and hypothyroidism. A strong genetic predisposition involved in the aetiology of these two diseases is therefore likely. Interestingly, there also appeared to be an inverse relationship of odds for the two diseases for some of the breeds since some breeds that had a high OR for DM or hypothyroidism had a lower OR for the other disease. This indicates that there may be different risk alleles/haplotypes for the two diseases. The possible aetiological relationship between canine DM and hypothyroidism should be further investigated.

Internal reconstruction of an extended full‐thickness duodenal mesenteric laceration through an antimesenteric duodenotomy in a dog

AbstractA 4‐year‐old English setter was presented for acute vomiting secondary to suspected consumption of a foreign body. Unusually extensive full‐thickness lacerations along the mesenteric border of the descending duodenum were present in association with devitalisation of portions of the ascending duodenum. To preserve the duodenum, total duodenectomy with associated biliary tract diversion was not considered. Ascending duodenectomy was performed. The descending duodenal mesenteric lacerations were managed using an internal primary closure technique through an antimesenteric duodenotomy. This particular technique is described, and its potential complications are discussed in this case report. While the outcome of this case report was excellent, more studies are needed before recommending this technique as part of the instrumentarium of duodenal repair.

The effects of activity, body weight, sex and age on echocardiographic values in English setter dogs

BackgroundBreed-specific reference intervals improve echocardiographic interpretation and thereby reduce misdiagnoses, especially in athletic breeds. ObjectivesThe objectives of the study were to examine transthoracic echocardiographic values in healthy adult English setter dogs and determine the effects of activity, body weight, sex and age on these values. Animals, materials and methodsOne hundred and one adult English setter dogs, recruited from local veterinary clinics and from the Norwegian English setter club, underwent routine transthoracic echocardiography. The population was stratified into two groups based on the reported level of activity. The effects of activity level, body weight, sex and age on echocardiographic variables were examined. Results were compared with published data from other breeds and from a pre-existing species-wide allometric model. ResultsOf the 100 dogs between 19 months and 10 years of age included in the study, 72 were reported as very active and 28 as less active. Echocardiographic intervals were calculated for body size–independent echocardiographic variables. The upper limits of the intervals for left-atrial-to-aortic ratios and normalised left ventricular volumes exceeded those of various, previously published studies of other breeds. Normalised left ventricular dimensions exceeded published allometric 95th percentile upper reference values in 13% of dogs in diastole and 32% of dogs in systole. More active dogs had larger cardiac dimensions than less active dogs; however, the activity level did not predict echocardiographic variables when included in a multiple regression model. ConclusionsThe study provides breed specific transthoracic echocardiographic values for English setter dogs, thereby contributing to improve diagnostic assessment of cardiac health in this breed.

Repair of a Divergent Elbow Dislocation with Distal Ulnar Fracture in a Dog Using TightRope and External Skeletal Fixation

AbstractThe aim of this study was to report the outcome in a 6-year-old male English Setter dog that suffered a combination of divergent elbow dislocation and open distal ulnar fracture. This study is a case report. After surgical reduction in the elbow luxation, the dog was treated with the TightRope fixation system used as replacement of the lateral collateral ligament, a radioulnar positional screw, and external skeletal fixation. Removal of all implants was performed 3 months postoperatively. The 6-month follow-up visit found the dog without lameness, displaying normal activity, and with normal elbow range of motion and normal Campbell's test. Successful surgical management was achieved with good long-term results using TightRope, a positional screw, and an external skeletal fixator.

Reconstructing Chinese Pidgin English phonology on the basis of written sources

"This paper is an attempt at outlining the phonology of Chinese Pidgin English, including its syllable structure, with an emphasis on the onset and the coda. Since Chinese Pidgin English is an extinct variety, the only available sources are written records such as magazine articles (e.g. in The Chinese Repository), literary works, travelogues, and letters. Reconstructing the phonology of Chinese Pidgin English on the basis of the orthography used in these sources raises the issues of the reliability of the sources and of the methodological implications. These are addressed in light of the caveats formulated by Mühlhäusler (1997), Baker and Winer (1999), Avram (2000), among others. In line with the principle of sociolinguistic accountability, all tokens in the samples of Chinese Pidgin English are included in the analysis as well as all the contexts where they might have appeared. In addition, a comparison will be made with other contemporary records of Chinese Pidgin English, with the phonology of Hong Kong English (Setter et al. 2010) and with the L2 phonology of Chinese learners of English. Such a comparison is certainly not the perfect equivalent of Rickford’s (1986) “feedback from current usage”, given that Chinese Pidgin English is no longer spoken. However, this approach is warranted by the so-called “uniformitarian principle” (Labov 1972), which posits that current patterns are similar to those that operated in the past."

Temporo-spatial and kinetic gait parameters in English setter dogs.

Walking analysis systems have begun to be used in veterinary medicine in recent years. The pressure-sensitive walkway is one of the systems through which we can obtain temporo-spatial and kinetic variables of walking. Therefore, the aim of this study was to investigate the walking characteristics of English Setter dogs using a pressure-sensitive system. Twenty-five English Setter dogs were included in the study. Temporo-spatial and kinetic gait parameters were obtained with the pressure-sensitive walkway system. Centre of pressure values were taken separately for the forelimbs and hindlimbs and were statistically analysed. The force values in the forelimb were found to be greater than in the hindlimb during walking. According to the results of dynamic pedobarographic evaluation, the highest-pressure values were found at the 2nd and 3rd digital pads for the forelimbs and on the 3rd and 4th digital pads for the hindlimbs. During the stance, 64.58% of the weight was found to be on the forelimbs. No difference was found between the forelimbs and the hindlimbs in centre of pressure analysis. As conclusion, the gait data obtained from the English Setter dogs can be used in future research to identify animals that may have neurological or orthopaedic problems.

A genome-wide association study of deafness in three canine breeds.

Congenital deafness in the domestic dog is usually related to the presence of white pigmentation, which is controlled primarily by the piebald locus on chromosome 20 and also by merle on chromosome 10. Pigment-associated deafness is also seen in other species, including cats, mice, sheep, alpacas, horses, cows, pigs, and humans, but the genetic factors determining why some piebald or merle dogs develop deafness while others do not have yet to be determined. Here we perform a genome-wide association study (GWAS) to identify regions of the canine genome significantly associated with deafness in three dog breeds carrying piebald: Dalmatian, Australian cattle dog, and English setter. We include bilaterally deaf, unilaterally deaf, and matched control dogs from the same litter, phenotyped using the brainstem auditory evoked response (BAER) hearing test. Principal component analysis showed that we have different distributions of cases and controls in genetically distinct Dalmatian populations, therefore GWAS was performed separately for North American and UK samples. We identified one genome-wide significant association and 14 suggestive (chromosome-wide) associations using the GWAS design of bilaterally deaf vs. control Australian cattle dogs. However, these associations were not located on the same chromosome as the piebald locus, indicating the complexity of the genetics underlying this disease in the domestic dog. Because of this apparent complex genetic architecture, larger sample sizes may be needed to detect the genetic loci modulating risk in piebald dogs.

STRING data mining of GWAS data in canine hereditary pigment-associated deafness

Most canine deafness is linked to white pigmentation caused by the piebald locus, shown to be the gene MITF (melanocyte inducing transcription factor), but studies have failed to identify a deafness cause. The coding regions of MITF have not been shown to be mutated in deaf dogs, leading us to pursue genes acting on or controlled by MITF. We have genotyped DNA from 502 deaf and hearing Australian cattle dogs, Dalmatians, and English setters, breeds with a high deafness prevalence. Genome-wide significance was not attained in any of our analyses, but we did identify several suggestive associations. Genome-wide association studies (GWAS) in complex hereditary disorders frequently fail to identify causative gene variants, so advanced bioinformatics data mining techniques are needed to extract information to guide future studies.STRING diagrams are graphical representations of known and predicted networks of protein-protein interactions, identifying documented relationships between gene proteins based on the scientific literature, to identify functional gene groupings to pursue for further scrutiny. The STRING program predicts associations at a preset confidence level and suggests biological functions based on the identified genes.Starting with (1) genes within 500 kb of GWAS-suggested SNPs, (2) known pigmentation genes, (3) known human deafness genes, and (4) genes identified from proteomic analysis of the cochlea, we generated STRING diagrams that included these genes. We then reduced the number of genes by excluding genes with no relationship to auditory function, pigmentation, or relevant structures, and identified clusters of genes that warrant further investigation.

Two-dimensional echocardiographic left- atrial-to-aortic ratio in healthy adult dogs: a reexamination of reference intervals

IntroductionLeft atrial-to-aortic ratios (LA:Ao) provide a body weight independent estimate of left atrial size. However, reference intervals were established with small sample populations and for only single points in the cardiac cycle. More robust reference intervals are warranted. AnimalsTwo hundred and thirty eight apparently healthy adult dogs. Materials and methodsLA:Ao measurements were obtained at 3 points in the cardiac cycle – maximal dimension, at the closing of the aortic valve (or just before opening of the mitral valve) (LA:AoMAX); minimal dimension, at the onset of the QRS complex (LA:AoMIN) and at the onset of atrial systole (LA:AoP). LA:AoMAX was obtained from right parasternal short and long-axis views, and LA:AoMIN and LA:AoP were obtained from the right parasternal short-axis view. Dogs were excluded from analyses of reference intervals if weight-based left atrial and left ventricular diastolic dimensions exceeded reference interval limits. Effects of breed and body weight on LA:Ao measurements were examined. ResultsUpper LA:Ao reference limits mostly agreed with previously published limits, although 10% of dogs had LA:AoMAX in the short-axis view exceeding 1.6. These dogs had smaller aortae than expected for their body weight, and included mostly boxers and English setters. Reference limits for LA:AoMIN and LA:AoP were smaller than those for LA:AoMAX in either view. No LA:Ao measurements were associated with body weight. ConclusionsReference limits were either confirmed or established for the common two-dimensional methods of assessing relative left atrial size in healthy dogs. Clinicians should use caution when diagnosing mild left atrial enlargement in certain dog breeds and should examine the weight-based aortic dimensions in such cases.

Congenital sensorineural deafness in English setters in the United Kingdom: prevalence and association with phenotype and sex

BackgroundThe English setter (ES) is predisposed to congenital sensorineural deafness (CSD). CSD prevalence and association with phenotype in the UK ES population are previously unreported.MethodsThe database of the authors’ institution...

Presumptive pituitary macroadenoma in an English Setter resembling human bobble-head doll syndrome

Head bobbing (HB) or idiopathic head tremor is a benign condition characterised by episodic uncontrolled head tremors that start and stop spontaneously or when dogs are focused on a goal-oriented...

Genomic characterization of the Braque Français type Pyrénées dog and relationship with other breeds.

The evaluation of genetic variability is a useful research tool for the correct management of selection and conservation strategies in dog breeds. In addition to pedigree genealogies, genomic data allow a deeper knowledge of the variability and genetic structure of populations. To date, many dog breeds, such as small regional breeds, still remain uncharacterized. Braque Français type Pyrénées (BRA) is a dog breed originating from a very old type of gun-dog used for pointing the location of game birds to hunters. Despite the ancient background, the knowledge about levels of genetic diversity, degree of inbreeding and population structure is scarce. This may raise concerns on the possibility that few inbred bloodlines may dominate the breed, and on its future health. The aim of this work was therefore to provide a high-resolution representation of the genome-wide diversity and population structure of BRA dogs, using the 170K genome-wide SNP array. Genome-wide polymorphisms in BRA were compared with those of other worldwide dog breeds. Between-dog relationships estimated from genomic data were very similar to pedigree relationships (Pearson correlation rg,a = 0.92). Results showed that BRA generally presents moderate levels of genetic diversity when compared with the major canine breeds. The estimated effective population size (recent Ne = 51) shows a similar declining pattern over generations as all other dog breeds, pointing at a common demographic history of modern canine breeds, clearly different from the demography of feral wolves. Multidimensional scaling (MDS), Bayesian clustering and Neighbor Joining tree were used to visualize and explore the genetic relationships among breeds, and revealed that BRA was highly differentiated and presented only low levels of admixture with other breeds. Brittany Spaniel, English Setter, Gordon Setter and Weimaraner dogs are the closest breeds to BRA. The exact reason for BRA being so divergent from other dog breeds, based on these results, is not yet clear. Further studies including additional ≪braccoid≫ breeds will be needed to refine the results presented here and to investigate the origin of the BRA breed. Nonetheless, the genome-wide characterization reported here provides a comprehensive insight into the genome diversity and population structure of the Braque Français, type Pyrénées breed.

T cell-lymphoma in the eyelid of a 9-year-old English Setter

BackgroundEyelid tumours are frequently found in dogs, most of these being benign. In case of an ulcerating eyelid tumour, malignancy must be considered. We report a unique case of a low-grade peripheral T-cell lymphoma in the eyelid of a 9-year-old English Setter.Case presentationA 9-year-old Setter presented with a 6-month history of an eyelid ulcer. A malignant eyelid neoplasm was suspected, and the lesion was surgically excised. No other treatment was applied, and 19 months after excision the dog was still well. Histopathology revealed a diffuse lymphocytic infiltrate in the eyelid skin. Ulceration of the epithelium was seen, and the underlying tumour was composed of round and poorly demarcated pleomorphic tumour cells. The cytoplasm was pale and the nuclei heterogeneous. Numerous mitoses were present. The tumour cells stained strongly for CD3. The final diagnosis was a peripheral T-cell lymphoma not otherwise specified (NOS).ConclusionsThis is the first described case of a solitary T-cell lymphoma NOS in the haired eyelid skin in a dog. Lymphoma should be considered in case of a persistent eyelid ulcer and a biopsy should be performed. T-cell lymphoma is generally an aggressive disease; however, indolent cases are well known, and as this case shows, complete excision of a solitary T-cell lymphoma can be curable. Canine cutaneous epitheliotropic T-cell lymphoma is an important differential diagnosis, which must be recognized as the prognosis is very poor and systemic treatment is mandatory. The sub-classification of canine lymphoma is not complete, and further studies are needed to identify lymphoma subgroups and provide treatment guidelines.

Variation in activity levels amongst dogs of different breeds: results of a large online survey of dog owners from the UK.

Regular physical activity is an important means of promoting health, both in people and their pets. Walking is the most common method used for dogs, but there is a lack of clarity on how much daily activity different breeds of dog require. Data from an online survey of UK dog owners were collected between June and August in 2014. The University of Liverpool Ethics Committee approved the project, and owners consented to data use. The initial dataset (17028 dogs) was first cleaned to remove erroneous data, and then edited to remove mixed breed dogs, leaving a total of 12314 dogs from known pedigree breeds. Other information collected included sex, age, neuter status, breed, and amount and frequency of exercise. Exercise frequency and duration were estimated across different breeds, and compared with Kennel Club recommendations, using χ2 tests and binary logistic regression. The online survey data indicated differences amongst breeds in the amount of walking reported (P<0·001). Afghan hounds were the least exercised breed, whilst breeds reportedly exercised most included: English setter, foxhound, Irish setter and Old English sheepdog. Gundogs were most likely to be walked once per d or more (P<0·001), whilst smaller dogs were more likely to meet their UK Kennel Club guidelines for dog walking (P<0·001). The frequency of dog walking varies both within and amongst breeds, and many do not currently receive the recommended amount of exercise. This may constitute a canine welfare problem and also have an impact on the physical activity levels of their owners.

Prevalence of Dog Erythrocyte Antigen 1 in 7,414 Dogs in Italy.

The study aim was to establish the prevalence of DEA 1, the most immunogenic and clinically important blood group in canine blood transfusion, in 7,414 dogs from Italy. The potential sensitization risk following a first transfusion and the acute reaction risk following a second transfusion given without a cross-matching and blood typing test were also calculated. Dogs tested were purebred (4,798) and mongrel (2,616); 38.8% were DEA 1 negative and 61.2% were DEA 1 positive. High prevalence for DEA 1 positive blood type was found in Ariegeois and English Setter, whereas German Shepherd and Boxer had higher DEA 1 negative blood type. Breeds with blood type never reported before included French Brittany Spaniel and Pug showing a high prevalence of DEA 1 positive type, while French Bulldog and West Highland White Terrier were more often DEA 1 negative. Just 48.8% of purebred and 13.9% of mongrel dogs were considered as prospective blood donors based upon their blood type. Most of the breeds had a sensitization risk of 20.0–25.0%. Rottweiler and Ariegeois had less risk of sensitization (9.4 and 4.2%) and the minor risk of an acute transfusional reaction (0.9–0.2%). The prevalence of DEA 1 positive and negative dogs in Italy agrees with most of the data already reported in the literature.

Natural zeolite (chabazite/phillipsite) dietary supplementation influences faecal microbiota and oxidant status of working dogs

We evaluated whether chabazite/phillipsite dietary supplementation might affect the faecal microbiota, oxidant and antioxidant status of working dogs at rest undergone to a trial test. Forty English Setter dogs were involved in two replicate trials. At each replicate, dogs were divided into two homogeneous groups (10 dogs/group). During a period of 28 days, diet was supplemented (Z group) or not supplemented (C group) with chabazite/phillipsite at the dose of 5 g/head/day. On day 29, dogs were subjected to a trial test. Faecal characteristics were assessed at 0 and 29 days (within two hours from the end of the trial test). Faecal consistency was not affected by dietary supplementation (p > .05). On day 29, Lactobacillus spp. and Enterococcus spp. counts were higher and Enterobacteriaceae were lower in Z than in C group (p < .05). At day 0 and at day 29, immediately before and within one hour from the end of the trial test, blood samples were recorded to evaluate the oxidant and antioxidant status. A reduction of 40% in thiobarbituric acid reactive substances (TBARS) levels was observed in Z compared to the C group (p < .05). Differences in nitric oxide and antioxidant enzymatic system levels were not significant (p > .05). Our results suggest that chabazite/phillipsite dietary supplementation, improves the intestinal microbiota ecosystem and may counteract the oxidative damage caused by physical stress in hunting dogs at the beginning of the working season.