Protein-energy Supplementation Research Articles

Performance and Metabolic Responses of Nellore Cows Subjected to Different Supplementation Plans during Prepartum

This study assessed the effects of different prepartum supplementation plans on Nellore cows’ performance, metabolic responses, and early offspring development. Thirty-nine pregnant Nellore cows (224 ± 2.67 days of pregnancy, 5.3 ± 0.29 years of age, body weight 520 ± 15.2 kg, initial body condition score 6.0 ± 0.07) were assigned to one of four treatments: a control group receiving only mineral mixture ad libitum, and three groups receiving daily protein-energy supplements of 2, 4, or 6 g/kg BW for 60 days prepartum. Weights and body condition scores were evaluated at the start of the experiment, 7 days before calving, and at 45 and 90 days postpartum. Cows supplemented with 4 and 6 g/kg BW showed improved body weight and body condition scores prepartum and postpartum and had a shorter service period (p < 0.05). The highest blood urea nitrogen concentrations were observed in cows receiving 6 g/kg BW (p = 0.0124). There was a reduction in blood urea nitrogen at calving for the 6 g/kg BW group, while the control group showed an increase (p < 0.001). Non-esterified fatty acids concentrations were lower 21 days before calving for the 4 and 6 g/kg BW groups compared to the control (p < 0.05) and decreased postpartum for all treatments (p < 0.001). No significant differences were observed in calf birth weight or performance. Supplementing with 4 g/kg BW of protein-energy is recommended to enhance metabolic health and overall performance.

Soybean molasses can be used as a substitute for corn in grazing beef cattle supplements during the rainy season.

Soybean molasses (SBMO) is a byproduct derived from the production of soy protein concentrate, obtained through solubilization in water and alcohol. The utilization of SBMO as an animal feed ingredient shows promising potential, primarily due to its low cost and as a potential energy concentrate. This study aimed to assess the intake, digestibility, ruminal parameters (pH and ruminal ammonia - NH3), nitrogen retention (NR) and microbial protein synthesis in grazing beef cattle supplemented with SBMO as a substitute for corn during the rainy season. Five Nellore (10-month-old) bulls with an average initial weight of 246 ± 11.2kg were utilized in a 5 × 5 Latin square design. The animals were housed in five paddocks, each consisting of 0.34ha of Marandu grass (Urochloa brizantha). Five isonitrogenous protein-energy supplements (300g crude protein [CP]/kg supplement) were formulated, with SBMO replacing corn at varying levels (0, 0.25, 0.50, 0.75, or 1.00g-1g). The supplements were provided daily at a quantity of 2.0kg-1 animal. The inclusion of SBMO at any level of corn substitution did not significantly affect the intake of pasture dry matter or total dry matter (P > 0.10). Likewise, the intake of CP and, consequently, the ruminal concentration of NH3 did not differ among the SBMO levels. Increasing the inclusion of SBMO did not have a significant impact on NR (P > 0.10), indicating that animals receiving supplements containing 100% SBMO as a substitute for corn may perform similarly to animals receiving supplements with 100% corn (0% SBMO). Soybean molasses represents a viable alternative energy source for grazing beef cattle during the rainy season and can entirely replace corn without adversely affecting animal nutritional performance.

Effect of a Prenatal Balanced Energy-Protein (BEP) Supplement on Gestational Weight Gain in Rural Ethiopia

Effect of a Prenatal Balanced Energy-Protein (BEP) Supplement on Gestational Weight Gain in Rural Ethiopia

Examining Energy and Macronutrient Intake Substitution in Home-Based Diets Among Pregnant Women in a Balanced Energy-Protein (BEP) Supplementation Trial in Sarlahi, Nepal

Examining Energy and Macronutrient Intake Substitution in Home-Based Diets Among Pregnant Women in a Balanced Energy-Protein (BEP) Supplementation Trial in Sarlahi, Nepal

Maternal Fortified Balanced Energy-Protein Supplementation Increases Diversity of the Maternal Gut Microbiome and the Capacity of Carbohydrate Uptake in Infant Gut

Maternal Fortified Balanced Energy-Protein Supplementation Increases Diversity of the Maternal Gut Microbiome and the Capacity of Carbohydrate Uptake in Infant Gut

Balanced Energy Protein Supplementation in Pregnancy: Adherence and Acceptability among Pregnant Women in Rural Ethiopia

BackgroundBalanced energy protein (BEP) supplementation in pregnant women in low-and middle-income countries may reduce the risk of stillbirth and low birth weight. ObjectivesThe objective of this study was to assess the adherence to and acceptability of a corn–soy blend (CSB) BEP product among pregnant women in rural Ethiopia. MethodsThis formative study was conducted from October to November 2018 among pregnant women in the rural Amhara region of Ethiopia prior to initiation of a clinical effectiveness study (ISRCTN: 15116516). We assessed adherence and acceptability of a micronutrient-fortified CSB BEP supplement among 40 pregnant women during a 4-wk utilization period. Acceptability was assessed using a 7-point Likert-style scale about the hedonic characteristics of the BEP product at 2 wk and 4 wk. Adherence was assessed by weekly monitoring and empty sachet counts for BEP consumption over 4 wk. ResultsAdherence to the BEP was, on average, 89% over the month-long pilot. The BEP product was rated favorably (mean Likert score >6 of 7) for the following domains: color, taste, odor, and likeability at mid and endpoints. Women found the product convenient to eat [mean (standard deviation [SD] = 5.9 (1.0))] and filling (mean (SD) = 6.1 (1.5) out of 7). Scores on acceptability and perception of the product remained stable throughout the duration of use. A majority of women (63%) reported consuming the BEP as a snack to supplement meals and splitting the serving over 2 or more sessions (68%). A quarter of respondents reported sharing the supplement with family members. ConclusionsAdherence and acceptability of the CSB BEP product were high among this population in rural Amhara, Ethiopia. This formative data was important to select the final product and shape the counseling and delivery of BEP in the parent study.

The effect of balanced energy-protein supplementation provided to lactating women on maternal and infant outcomes: study protocol for a prospectively planned individual patient data (IPD) meta-analysis

Abstract* Background The high prevalence of infant stunting and maternal undernutrition in low- and middle-income countries poses a significant public health threat. The World Health Organization recommends balanced energy-protein (BEP) supplementation to pregnant women from populations with a high prevalence of underweight (prepregnancy BMI <18.5 kg/m2), leaving a notable gap in guidance for lactating women. Therefore, we established the Maternal BEP Studies Harmonization Initiative (BEP Initiative) to investigate the impact of BEP supplementation given to pregnant and/or lactating women on maternal and infant outcomes by synthesizing data from multiple clinical trials. This is a study protocol for our prospective individual participant data (IPD) meta-analysis on BEP lactation trials. Methods Data from four randomized controlled trials that include mother-infant dyads in India (n=816), Pakistan (n=957), Burkina Faso (n=800), and Nepal (n=726) will be pooled and analysed. Women were randomized to BEP (one trial had a third arm with maternal BEP plus infants receiving azithromycin) or control groups at baseline (during the first week) and received the intervention through six months postpartum. A one-stage IPD meta-analysis will be done using mixed-effects linear and log-binomial regression models to account for between-trial heterogeneity. The primary outcome of infant length-for-age z scores (LAZ) and secondary outcomes of maternal and infant indicators of nutritional status, all at six months of age, will be examined. Also, we will assess baseline characteristics as covariates and effect modifiers for the BEP to outcome relationship. Discussion This prospective IPD meta-analysis uses a one-stage IPD meta-analysis, which allows for higher statistical power to examine outcomes, more flexibility in defining variables, and can examine effect modifiers, which could inform which individuals or populations may benefit more from BEP given during lactation.

Effect of maternal postnatal balanced energy protein supplementation and infant azithromycin on infant growth outcomes: an open-label randomized controlled trial

BackgroundMaternal undernutrition is a direct risk factor for infant growth faltering. ObjectivesWe evaluated the effect of postnatal balanced energy protein (BEP) supplementation in lactating women and azithromycin (AZ) in infants on infant growth outcomes. MethodsA randomized controlled superiority trial of lactating mother–newborn dyads was conducted in Karachi, Pakistan. Mothers intending to breastfeed their newborns with mid-upper arm circumference of <23 cm and live infants between 0 and 6 d of life were randomly assigned to 1 of 3 arms in a 1:1:1 ratio. Lactating mothers in the control arm received standard-of-care counseling on exclusive breastfeeding, nutrition, infant immunization, and health promotion plus iron-folate supplementation until the infant was 6 mo old. In intervention arm 1, mothers additionally received two 75-g sachets of BEP per day. In intervention arm 2, along with the standard-of-care and BEP to the mother, the infant also received 1 dose of azithromycin (20 mg/kg) at the age of 42 d . The primary outcome was infant length velocity at 6 mo. The total sample size was 957 (319 in each arm). ResultsFrom 1 August, 2018 to 19 May, 2020, 319 lactating mother–newborn dyads were randomly assigned in each arm, and the last follow-up was completed on 20 November, 2020. The mean difference in length velocity (cm/mo) between BEP alone and control was 0.01 (95% confidence interval [CI]: −0.03, 0.06), BEP plus AZ and control was 0.08 (95% CI: 0.03, 0.13), and between BEP + AZ and BEP alone was 0.06 (95% CI: 0.01, 0.11). There were 1.46% (14/957) infant deaths in the trial, and 17.9% (171/957) nonfatal events (injectable treatment and/or hospitalizations) were recorded. ConclusionsPostnatal maternal BEP supplementation and infant AZ administration could modestly improve infant growth outcomes at 6 mo, suggesting potential benefits in simultaneously addressing maternal and infant undernutrition.This trial was registered at clinicaltrials.gov as NCT03564652.

Nutritional Performance of Grazing Beef Cattle Supplemented with High-Protein Distillers' Dried Grain.

The objective was to evaluate the effects of including high-protein distillers dried grains (HP-DDG; 430 g/CP) in supplements for beef cattle in an intensive finishing pasture system. Five Nellore bulls with an average body weight (BW) of 413.5 ± 32 kg were distributed in a 5 × 5 Latin square design. The animals were randomly allocated to Marandu palisade grass paddocks (Urochloa brizantha cv. Marandu), with 0.32 ha each. Protein-energy supplements were evaluated and formulated with different replacement levels (0, 250, 500, 750 and 1000 g/kg) of soybean meal (SBM) by HP-DDG. Supplements were offered once a day in the amount of 6.0 kg/animal. Replacing SBM with HP-DDG had no effect (p > 0.10) on the intake of total and pasture DM, OM, CP, NDFap, digestible organic matter (DOM), metabolizable protein and CP:DOM ratio. Total and pasture DM intake averaged 6.07 and 11.54 kg/day, respectively. Replacing SBM with HP-DDG reduces and increases, respectively, the intake of degradable (RDP) and undegradable (RUP) protein in the rumen (p < 0.10) with a consequent linear reduction in ruminal ammonia concentration (RAN), nitrogen excretion in urine and serum N concentration (SUN) (p < 0.10). In supplements offered in the amount of 6.0 kg animal/day, SBM can be completely replaced by HP-DDG.

Perceived progress toward scale of 14 maternal, newborn, child health, and nutrition (MNCHN) assets at subnational level in Burkina Faso, Ethiopia, India, Kenya, and Nigeria.

Inequitable coverage of evidence-based MNCHN interventions is particularly pronounced in low and middle income countries where access and delivery of these interventions can vary dramatically at the subnational level. We conducted health system assessments in nine subnational geographies in five countries (Burkina Faso, Ethiopia, India, Kenya and Nigeria) to explore progress toward scale of 14 evidence-based MNCHN interventions (iron-folic acid, oxytocin, magnesium sulfate, misoprostol; 7.1% chlorhexidine for umbilical cord care, neonatal resuscitation, kangaroo mother care, community regimen for the treatment of possible severe bacterial infection; amoxicillin dispersible tablets, multiple micronutrient supplements, balanced energy protein supplementation, early and exclusive breastfeeding, feeding of small and sick newborns, and management of severe and moderate acute malnutrition in children less than five years old). Between March and October 2021, we conducted key informant interviews with a purposive sample of 275 healthcare providers and 94 district health management (DHMT) staff to better understand bottlenecks, facilitators and uptake of the interventions across varied subnational settings. Across all interventions and geographies, providers and DHMT staff perceived lack of robust HMIS data as the most significant barrier to scale followed by weak facility infrastructure. DHMT staff viewed limited budget allocation and training as a much larger barrier than healthcare providers, most likely given their purview as subnational managers. Healthcare providers were focused on supply chain and staffing, which affect workflows and service provision. Understanding provider and health facility management views of why interventions do or do not advance towards effective coverage can assist in creating enabling environments for the scale of best practices. These types of data are most helpful when collected at the subnational level, which allows for comparisons both within and between countries to show health disparities. Importantly, this strategic data collection can provide a starting point for improvement efforts to address existing health system gaps.

Effects of Feed Ingredients with Different Protein-to-Fat Ratios on Growth, Slaughter Performance and Fat Deposition of Small-Tail Han Lambs.

The purpose of this study was to investigate the effects of feed ingredients with different protein-to-fat ratios on growth, slaughter performance and meat quality of Small-Tail Han lambs. Forty-five Small-Tail Han lambs (♂) (BW = 34.00 ± 2.5 kg, age = 120 ± 9 d) were randomly divided into groups with three different experimental treatments: (1) PF 5, with the ratio of protein to fat (CP:EE) of 50 to 5; (2) PF10, CP: EE = 50:10; (3) PF20, CP: EE = 50:20. Each treatment group had 15 lambs, and each sheep was a repeat. This experiment lasted for 65 days, with feed intake recorded daily, and animals being weighed on days 0, 30, and 65. At the conclusion of the experiment, eight lambs from each group were slaughtered to assess slaughter performance and meat quality. The results showed that the average daily gain (ADG) of the three groups were 315.27, 370.15 and 319.42 g/d, respectively. The PF10 group had the highest ADG (370.15 g) (p < 0.05). Forestomach weights (1216.88 g) of the PF10 group were significantly higher than those of the other groups (p < 0.05). There were no differences (p > 0.05) in fat percentages in various parts of body among treatments. Feed conversion of the PF10 group was higher (p < 0.05) than that of PF 5 and PF 20 groups. Furthermore, the PF10 group had a higher (p > 0.05) carcass weight and slaughter rate and there were few differences between the other groups in terms of dry matter intake, meat quality, organ weight, and fat deposition (p > 0.05). The protein-energy supplement with protein-to-fat ratio, PF10 appeared to be more desirable to promote the growth and development in Small-Tail Han Lambs.

Effects of Early Weaning on Performance and Carcass Quality of Nellore Young Bulls.

This study compared early weaning (EW; 150 days) with conventional weaning (CW; 240 days) in Nellore young bulls, evaluating performance, carcass characteristics, and meat quality. A total of 74 non-castrated male calves were divided into two weaning strategies: EW (n = 37) and CW (n = 37). During the growth phase, which lasted 454 ± 14 d for EW calves and 359 ± 16 d for CW calves, animals received a protein-energy supplement at a ratio of 5 g per kg of body weight while grazing Brachiaria brizantha cv. Marandu. The animals were managed for an 87d finishing phase in three collective feedlot pens, with a 3-week adaptation protocol, starting with corn silage to a concentrate ratio of 55:45 and reaching a ratio of 30:70 in the final diet. Body weight, average daily gain (ADG), dry matter intake (DMI), feed efficiency (FE), carcass characteristics, and meat quality were evaluated. The EW group was approximately 44 kg lighter than the CW at the time of conventional weaning (p < 0.001). However, this weight difference did not influence ADG, DMI, and FE in the finishing phase. No significant differences were observed in carcass characteristics such as yield percentage, loin area, subcutaneous fat thickness, and meat quality, except for the weight of primal cuts, which was greater in the CW group (p < 0.001). Thus, although calves weaned early are lighter throughout subsequent production phases than those weaned conventionally, performance, efficiency, carcass yield, and meat quality are not affected.

Evaluation of Muscle Long Non-Coding RNA Profile during Rearing and Finishing Phase of Bulls Subjected to Different Prenatal Nutritional Strategies.

Maternal nutrition has the ability of influence critical processes in fetal life, including muscle development. Also, in this period, epigenetic sensitivity to external stimuli is higher and produces long-lasting effects. Thus, the aim of this study was to investigate epigenetic mechanisms, including the identification and characterization of long non-coding RNA (lncRNA) from animals that had undergone different strategies of prenatal supplementation. A group of Nellore cows (n = 126) were separated into three nutritional plans: NP (control)-Not Programmed, without protein-energy supplementation; PP-Partially Programmed, protein-energy supplementation in the final third of pregnancy; and CP-Complete Programming, protein-energy supplementation during the full period of gestation. A total of 63 male offspring were used in this study, of which 15 (5 per treatment) had Longissimus thoracis muscle at 15 (biopsy) and 22 months (slaughter). Biopsy samples were subjected to RNA extraction and sequencing. Differential expression (DE) of remodeling factors and chromatin-modifying enzyme genes were performed. For the identification and characterization of lncRNA, a series of size filters and protein coding potential tests were performed. The lncRNAs identified had their differential expression and regulatory potential tested. Regarding DE of epigenetic mechanisms, no differentially expressed gene was found (p > 0.1). Identification of potential lncRNA was successful, identifying 1823 transcripts at 15 months and 1533 at 22 months. Among these, four were considered differentially expressed between treatments at 15 months and 6 were differentially expressed at 22 months. Yet, when testing regulatory potential, 13 lncRNAs were considered key regulators in the PP group, and 17 in the CP group. PP group lncRNAs possibly regulate fat-cell differentiation, in utero embryonic development, and transforming growth factor beta receptor, whereas lncRNA in the CP group regulates in utero embryonic development, fat-cell differentiation and vasculogenesis. Maternal nutrition had no effect on differential expression of epigenetic mechanisms; however, it seems to impair lncRNA regulation of epigenetics.

Influência de suplementos de baixo e médio consumo na fase de crescimento de novilhos mantidos em pastagem tropical e terminados em confinamento

ABSTRACT: The objective was to evaluate the animal production and ingestive behavior of the steers fed different supplements during the growth phase in Aruana grass, and the influence of this supplementation on production performance in feedlot phase. The treatments consisted of three supplements during the growing phase: mineral salt (0.12 g/kg of body weight - BW), low intake energy-protein supplement (1.26 g/kg of BW), and medium intake (3.76 g/kg of BW). Forage canopy characteristics, animal production, and animal behavior on pasture were evaluated in a completely randomized design with three replicates (paddocks). The titanium dioxide marker was used to determine the intake and apparent digestibility during the pasture phase. The production performance in feedlot was evaluated in a completely randomized design with eight replicates (animals). Twenty-four Aberdeen Angus steers (15 ± 1.5 months old; 364.8 ± 21.7 kg BW) were used. At the end of the growth phase the animals were confined and fed a single diet. Dry matter, crude protein, and neutral detergent fiber (NDF) contents of forage and intake were not influenced by the treatments. The average daily gain was higher for the medium intake supplement in pasture phase. The apparent digestibility of NDF (P = and chewing rate were higher for the medium intake supplement. The average daily gain and fat thickness of carcasses were higher for the medium intake supplement in the feedlot phase. The supplements of medium intake increase animal productivity during the growing phase on pastures and cattle’s finishing performance in intensive production systems.

Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming.

This study investigated changes in meat and subcutaneous fat metabolomes and possible metabolic pathways related to prenatal nutrition in beef cattle. For this purpose, 18 Nellore bulls were used for meat sampling and 15 for fat sampling. The nutritional treatments during the gestation were: NP-not programmed or control, without protein-energy supplementation; PP-partially programmed, with protein-energy supplementation (0.3% of body weight (BW)) only in the final third of pregnancy; and FP-full programming, with protein-energy supplementation (0.3% of BW) during the entire pregnancy. The meat and fat samples were collected individually 24 h after slaughter, and the metabolites were extracted using a combination of chemical reagents and mechanical processes and subsequently quantified using liquid chromatography or flow injection coupled to mass spectrometry. The data obtained were submitted to principal component analysis (PCA), analysis of variance (ANOVA), and functional enrichment analysis, with a significance level of 5%. The PCA showed an overlap between the treatments for both meat and fat. In meat, 25 metabolites were statistically different between treatments (p ≤ 0.05), belonging to four classes (glycerophospholipids, amino acids, sphingolipids, and biogenic amine). In fat, 10 significant metabolites (p ≤ 0.05) were obtained in two classes (phosphatidylcholine and lysophosphatidylcholine). The functional enrichment analysis showed alterations in the aminoacyl-tRNA pathway in meat (p = 0.030); however, there was no pathway enriched for fat. Fetal programming influenced the meat and fat metabolomes and the aminoacyl-tRNA metabolic pathway, which is an important candidate for the biological process linked to meat quality and related to fetal programming in beef cattle.

Strategies of virginiamycin supplementation in the postweaning phase on growth performance and carcass quality of beef cattle.

The present study evaluated the effects of supplementing VM in grazing cattle during the rearing phase on performance and carcass quality of beef cattle in the finishing phase. Two experiments with a randomized block design were conducted in consecutive years to contrast two post-weaning supplementation strategies using VM at 45 mg/100 kg body weight (BW). In the first year, treatments were protein supplement in the dry season and mineral supplement in the rainy season versus the addition of VM both in the protein and mineral supplements. In the second year, was contrasted with protein supplement in the dry season and protein-energy supplement in the rainy season. Performance, carcass traits, and carcass quality were evaluated at the end of both phases. In Year 1, adding VM in mineral supplement increased final backfat thickness (P=0.05), backfat gain (P=0.06), final rump fat thickness (P=0.02), and rump fat gain (P=0.01). In the finishing phase, VM-treated cattle had a greater dry matter intake (P=0.03) and tended to show a greater backfat thickness than non-treated cattle (P=0.07). In Year 2, no VM effects were observed on post-weaning phase performance and carcass traits. However, cattle-fed VM during the post-weaning phase tended to show a lower feed conversion ratio (P=0.09) and had a significantly higher gross feed efficiency (P=0.03) than non-treated cattle at slaughter. Virginiamycin supplementation during rearing on pasture improves performance and carcass fattening in the growth phase and has a residual effect in the finishing phase that may reflect greater backfat thickness and gross feed efficiency.

Assessing implementation outcomes for launching balanced energy protein supplementation: A formative study in rural Bangladesh

AbstractBalanced energy protein (BEP) supplementation is an efficacious intervention in pregnancy for improving birthweight and is recommended by World Health Organization (WHO) in countries with high maternal undernutrition. Few countries have implemented BEP programmes due in part to high cost, lack of data on acceptability and feasibility, and complexity of delivery. We sought to address implementation gaps in BEP interventions through a formative study designed to understand implementation outcomes. We conducted 52 in‐depth interviews and 8 focus‐group discussions with married women of reproductive age, family members, health care providers and pharmacists in three unions of the Gaibandha district in rural Bangladesh. Interviews were translated and transcribed in English and analysed using an analytic framework for implementation science in nutrition. BEP was viewed as an acceptable and appropriate intervention to combat undernutrition in this setting. There was a lack of clarity on who should or could be responsible for providing/distributing BEP in a way convenient to mothers. Many participants preferred door‐to‐door delivery and thought this approach could address social and gender inequities, but providers mentioned already being overworked and worried about adding new tasks. Participants were concerned about the affordability of BEP and opportunity costs associated with travel to proposed distribution sites such as ANC or pharmacies. Women in these communities do not always have the agency to travel without supervision or make purchasing decisions. BEP supplementation is a complex intervention; future trials seek to assess ways to overcome these implementation challenges and inform a long‐term systems‐owned BEP intervention.

Fetal Programming and Its Effects on Meat Quality of Nellore Bulls.

This work aimed to evaluate the effects of prenatal nutritional stimulation at different pregnancy stages on carcass traits and meat quality in bovine progeny. For this purpose, 63 Nellore bulls, born from cows submitted to three nutritional plans, were used: not programmed (NP), which did not receive protein supplementation; partially programmed (PP), which had protein-energy supplementation (0.3% of mean body weight of each batch) only in the final third of pregnancy; and full programming (FP), which received supplementation (0.3% of mean body weight of each batch) throughout pregnancy. The averages of parameters were submitted to the ANOVA, and the supplementation periods, which were different when p value < 0.05, were compared. Carcass weights and rib eye area (REA) did not differ between treatments (p > 0.05), but subcutaneous fat thickness (SFT) showed a tendency (p = 0.08) between groups. For lipids and marbling, no differences were found (p > 0.05). In the analyses of maturation time and shelf life, no difference was observed between treatments. However, there was a tendency between treatments at 14 days of maturation time for cooking loss (CL) (p = 0.08). Treatments did not affect shear force in the progenies (p > 0.05). Fetal programming had no effect on the meat quality of Nellore bulls.

PSVII-18 Applying the Marbling Window Concept to Enhance Intramuscular Fat Development in Beef Cattle

Abstract The increase in marbling deposition without increasing the overall fatness of the carcass is one of the most challenge goals in meat industry. Previous studies have suggested the nutritional interventions at the neonatal stages as a strategy to enhance the commitment of muscular mesenchymal stem cells to adipogenesis, which may contribute to enhance marbling deposition at the finishing phase. As such the objective of this study was designed the evaluate the impact nutrient supplementation during the pre-weaning stage of beef calves has on intramuscular adipogenic determination. Thirty-four female calves were assigned to two experimental treatments: Control (CON, n = 17), where calves received only a mineral mixture as a supplement; Supplemented (SUP, n = 17), where calves received an energy-protein supplement (0.5 kg·100 kg of BW-1·day-1; the same mineral mixture was used in the CON supplement). Calves were supplemented from 100 to 250 days of age. Once animals reached 250 days of age, skeletal muscle tissue samples were biopsied from the 10th and 11th rib and used for identification of mesenchymal stem cells and preadipocytes through immunofluorescence, mRNA and protein abundance of adipo-fibrogenic markers, and abundance of candidate miRNA controlling expression of adipogenic commitment. Data were analyzed considering the completely randomized design of the experiment. Heifers were randomized across paddocks, and paddocks were randomized across treatments. The PROC MIXED of SAS 9.2 was used for all analyses. The average daily gain (P = 0.08) and final body weight (P = 0.07) tended to be greater in SUP than CON calves. The number of fibro-adipogenic progenitor cells (P = 0.69) did not differ between treatments, while a greater number of intramuscular pre-adipocytes were observed in SUP than CON calves (P = 0.01). The expression of miRNA-4429 (P = 0.20) did not differ between treatments, while the expression of miRNA-129-5p (P = 0.09) and miRNA-129-2-3p (P = 0.05) tended to be greater in CON than SUP calves. Our findings demonstrate that energy-protein supplementation from 100 to 250 days of age is capable to enhance intramuscular adipogenesis in beef cattle. Moreover, our results suggest that regulation of adipogenic commitment occurs by post-transcriptional regulation through miR-129 family.

PSX-13 Effects of Different Supplementations During the Rainy Season on Nitrogen Metabolism of Beef Cattle Steers on Tropical Pasture

Abstract Phytogenic additives are used as food additives in ruminant nutrition due to their potential to alter the nitrogen (N) excretion route and minimize N emissions into the environment. This study aimed to evaluate the effect of two levels of supplementation plus a mixture of phytogenic additives (essential oils, saponins and spices) on the efficiency of N utilization by beef cattle in tropical pastures. The experiment was conducted at the Beef Cattle Sector of UNESP, Jaboticabal-BR, and lasted 84 days. Nine castrated Nelore steers (body weight 262 ± 31.2 kg) cannulated in the rumen were distributed in 3 simultaneous 3 × 3 Latin square designs. The animals were kept in pasture of Urochloa brizantha cv. Marandu during the rainy season. Treatments included ad libitum mineral supplementation (MS) as a control group, mineral supplementation added 0.1% of body weight per day plus Cargill phytogenic mixture (AMSP) and energy-protein supplementation, 0.3% of body weight per day plus Cargill phytogenic mixture (EPSP). Point urine and fecal samples were collected in the last three days of each experimental period. The concentration of total nitrogen in the urine was analyzed by the Kjeldahl procedure. The nitrogen composition of forage, supplements and feces was estimated by near-infrared spectroscopy (NIRS) using a modular FT-NIR spectrometer (NIRFlex N500, Büchi Labortechnik AG, Flawil, Switzerland). Nitrogen retention was calculated using the following equation: NR = N intake (g/d) – [N excretion in feces (g/d) + N excretion in urine (g/d)]. The nitrogen metabolism data were analyzed as a 3 × 3 Latin square with 3 replications, balanced for residual effects. Considering as fixed effect the treatment, and as random effect the Latin square, period, steer, steer × period interaction, and residues corresponding to the model. The data obtained were submitted to ANOVA and Tukey's test (P ≤ 0.05), using the statistical program R, version 3.4.4. The addition of phytogenic additives in the supplements led to decreased (P &amp;lt; 0.036) urinary excretion of N (g/day) from 41.16 g/day to 28.38 g/day and urinary excretion of N (% of N intake) from 30.66% to 23.21%, but did not differ between the different levels of phytogenic supplementation (Table 1). In addition, retained N (% of N intake) was greater (P &amp;lt; 0.01) in animals supplemented with AMSP and EPSP compared with MS, increasing N retention from 26.78% to 33.88%. This study indicates that the different levels of supplementation plus the addition of the phytogenic mixture improved the efficiency of N utilization by decreasing the excretion of N in the urine and increasing the retained N compared with MS supplementation.