Abstract Fescue toxicosis decreases beef cattle performance in a multitude of ways, including decreased health and immunity. Cattle challenged by fescue toxicosis are often grazing in an environment favorable for development of gastrointestinal nematodes (GIN). Interactions of fescue toxicosis and parasite challenge may result in further performance and economic losses. Many beef producers treat animals regularly for GIN parasites with little insight on necessity or effectiveness of anthelmintic products. Overuse of anthelmintics can result in development of anthelmintic resistance in GIN populations. To minimize the development of anthelmintic resistance, implementation of selective deworming practices should be considered. However, level of parasite burden and effects of fescue toxicosis on parasite burden need to be better understood in beef cattle herds before recommendations can be offered. Therefore, the objective was to investigate the impact of fescue toxicosis on GIN parasite infection rates through fecal egg counts (FEC), body weight (BW), hematocrit (HCT), and body condition score (BCS). Starting in April, for 14 consecutive weeks post-weaning, pregnant Angus-based cows (2 to 4 years old, n = 65) were rotationally grazed on either novel endophyte (EN) fescue or endophyte-infected (EI) fescue pastures. Hematocrit, BCS, and BW were assessed weekly, while FEC were measured bi-weekly to monitor the physiological changes as a result of consuming endophyte-infected tall fescue. All data were analyzed using Proc MIXED in SAS with repeated measures. Statistical significance was declared at P ≤ 0.05 and a trend at 0.10 > P > 0.05. Overall, FEC were low (4.2 ± 0.9 eggs/gm) in these mature cattle and grazing EI or EN fescue did not have any overall effect (P > 0.05) on the FEC. However, FEC decreased (P < 0.05) over the course of the study from week 1 to week 13. Body weight, HCT, and BCS were not affected (P > 0.05) by the type of fescue grazed. There was a tendency (P < 0.10) for 2-3 and 4-6 year old cattle to have lower BW when grazing EI compared with EN fescue while cattle >7 were not affected. Cattle gained BW (P < 0.05) over the 14-week study (471.4 and 527.7 ± 0.6 kg, 0 and4 week respectively). For cattle grazing EI fescue, HCT linearly decreased (P < 0.05) from week 1 to week 14 whereas cattle grazing EN fescue had a linear increase (P < 0.05) in HCT from week 1 to week 14. More research is warranted to investigate beef cattle FEC and the potential impact of fescue type on FEC in younger beef cattle.