Event Abstract Back to Event The role of myosin phosphatase in the synaptic transmission in the rat ventral cochlear nucleus Áron Kőszeghy1*, Balázs Pál1, Géza Szűcs1, Beata Lontay2, Ferenc Erdodi2 and Zoltan Rusznak1 1 Departments of Physiology , Medical and Health Science Centre, University of Debrecen, Hungary 2 Departments of Medical Chemistry, Medical and Health Science Centre, University of Debrecen , Hungary It has been suggested that myosin phosphatase (MP) may have a regulatory role in neurotransmission. In this work involvement of MP was investigated in the synaptic action occurring in a mammalian giant synapse (endbulbs of Held) situated in the rat ventral cochlear nucleus (VCN). Whole-cell measurements were performed on bushy neurones using a thin-slice preparation. To evoke the activation of the glutamatergic synapse situated between the the acoustic nerve fibers and the cell bodies of the bushy cells, the root of the auditory nerve was stimulated (50 Hz), and the resulting excitatory postsynaptic currents (EPSCs) were recorded. To assess the significance of the MP, the effects of tautomycetin (a protein phosphatase-1-specific inhibitor) and Y27632 (a rho-kinase-specific inhibitor) were determined on various parameters of the EPSCs. When the slices were pre-incubated in 5 µM tautomycetin, the amplitude of the first EPSC was decreased, whereas the relative amplitude of the subsequent EPSCs was increased. The decay tau of the EPSCs was also significantly increased. In contrast, exactly the opposite actions have been noted in the case of Y27632 application (10 µM): the amplitude of the first EPSC increased, the relative amplitudes of the subsequent EPSCs reduced, and the decay tau was increased. Our results suggest that MP has important roles in the regulation of the synaptic transmission occurring in the giant synpse ofthe VCN, and the regulatory effect is exerted via both pre and postsynaptic mechanisms. Supported by the OTKA (K-68416 and K-72812) Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Kőszeghy Á, Pál B, Szűcs G, Lontay B, Erdodi F and Rusznak Z (2009). The role of myosin phosphatase in the synaptic transmission in the rat ventral cochlear nucleus. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.236 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2009; Published Online: 13 Mar 2009. * Correspondence: Áron Kőszeghy, Departments of Physiology , Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary, aron.koeszeghy@meduniwien.ac.at Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Áron Kőszeghy Balázs Pál Géza Szűcs Beata Lontay Ferenc Erdodi Zoltan Rusznak Google Áron Kőszeghy Balázs Pál Géza Szűcs Beata Lontay Ferenc Erdodi Zoltan Rusznak Google Scholar Áron Kőszeghy Balázs Pál Géza Szűcs Beata Lontay Ferenc Erdodi Zoltan Rusznak PubMed Áron Kőszeghy Balázs Pál Géza Szűcs Beata Lontay Ferenc Erdodi Zoltan Rusznak Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.