Conditions Of Emotional Stress Research Articles

Evaluation of burnout and stress perception levels of Turkish dental laboratory technicians according to affecting factors during the COVID-19 pandemic

Background The coronavirus disease-2019 (COVID-19) pandemic has caused healthcare professionals to face unequal acute workplace stress and burnout. This study aimed to analyze the potential impact of COVID-19 on the burnout and associated emotional stress conditions of Turkish dental technicians. Methods A 20-question demographic scale, Maslach Burnout Inventory (MBI), Sense of Coherence-13 (SoC-13), and Perceived Stress Scale-10 (PSS-10) were used to obtain data. A total of 152 participants answered these surveys directly and reported their stress burnout levels during the COVID-19 pandemic. Results Of all participants who agreed to participate in the survey, 39.5% were females and 60.5% were males. Regardless of demographic variables, the MBI-total (37.2 ± 11.71), SoC-13 total (53.81 ± 10.29), and PSS-10 total (21.25 ± 5.5) scores indicated moderate burnout, SoC, and perceived stress levels. According to sub-scores of the MBI; mean emotional exhaustion and depersonalization indicate low-level burnout, and mean personal accomplishment indicates moderate burnout. Long working hours increase burnout. No significant differences were observed according to demographic variables, except for work experience. A positive correlation was found between perceived stress and burnout. Conclusions The findings showed that dental technicians working during the COVID-19 pandemic are influenced by emotional stress due to the outcomes of the pandemic. One reason for this situation might be the long working hours. Working arrangements, under-controlled disease risk factors, and lifestyle changes may improve stress levels. Key Messages COVID-19 outbreak exposed psychological returns to the general population, and especially to healthcare workers. Questionnaire method was applied to evaluate the burnout and stress levels among dental laboratory technicians during COVID-19 outbreak. Moderate levels of burnout and stress perception were detected. Long working time was one of the effective factors.

Design, Synthesis, and Study of the Anxiolytic Activity of New Pyrrolo[1,2-<i>a</i>]pyrazine-Containing TSPO Ligands

A new group of 1-phenylpyrrolo[1,2-a]pyrazine-3-carboxamides, in which substituents at the amide nitrogen atom are alkyl, benzyl or alkoxyphenylalkyl groups, amino acid residues or their derivatives, has been obtained in this work. The synthesized compounds have high theoretical affinities for the 18 kDa translocator protein (TSPO) and a favorable profile of ADMET characteristics, which makes them promising for drug development. Anxiolytic activity was detected for eight compounds at doses of 0.1–5.0 mg/kg when administered intraperitoneally under conditions of emotional stress in the open field test in Balb/c mice and in the elevated plus maze test in ICR mice. N-Benzhydryl-1-phenylpyrrolo[1,2-a]pyrazine-3-carboxamide was selected as a lead compound for further development as a potential anxiolytic agent, which showed the presence of anti-anxiety activity in a wide range of doses in both tests used.

Lymphocyte Index of Peripheral Blood in Rats at Different Stages of the Post-Stress Period under Conditions of Antigenic Exposure and Injection of Lipopolysaccharide.

Changes in the Shaganin lymphocyte index (ratio of the number of lymphocytes to segmented neutrophils) in the peripheral blood of rats after intraperitoneal administration of LPS (100 μg/kg) at the end of a single stress exposure in a model of 24-h restraint stress were studied. The lymphocyte index was analyzed 3 h later, on the 1st and 8th days after the stress load. Immobilization was accompanied by a decrease in this parameter 3 h after exposure. One day after the stress load, an increase in the lymphocyte index was noted, which remained on the 8th day of observation. LPS injection did not affect the changes in this parameter caused by 24-h immobilization on the 1st and 8th days of the study, but prevented a pronounced increase in the lymphocyte index on the 1st day after the stress load. The data obtained expand the existing scientific understanding of the specificity of the involvement of immunomodulatory substances in the implementation of adaptive-compensatory processes in mammals under conditions of emotional stress.

The influence of Combination Therapy Progressive Muscle Relaxation and Deep breathing Relaxation against Stress Levels of diabetics in Prolanis BPJS The Health Of The City Of Palangka Raya

Diabetes mellitus is one of the diseases with great impact on health and society, not only for its high prevalence but also for chronic complications and high mortality.. The psychological impact of diabetes began to be felt by the patient since he was diagnosed the doctor. Patients begin to experience psychological disorders such stress on himself related to the care and treatment should be undertaken. Conditions of emotional stress that require proper handling in order not to fall on the more severe conditions. This study aims to identify the influence of combination therapy progressive muscle relaxation and deep breathing relaxation against stress levels of patients with diabetes mellitus in Prolanis BPJS Kesehatan Kota Palangka Raya. The design used in this study using the design of the study : “PRE-TEST-POST-TEST DESIGN WITH CONTROL GROUP”. In this study population was patients with diabetes mellitus in Prolanis BPJS Kesehatan Kota Palangka Raya. The sampling technique in this study using total sampling technique, as many as 48 people. This research is expected to contribute positively to the management of emotional stress in patients with diabetes mellitus.

Transcriptome-Wide Identification of G-to-A RNA Editing in Chronic Social Defeat Stress Mouse Models.

Emerging evidence suggests that RNA editing is associated with stress, neurological diseases, and psychiatric disorders. However, the role of G-to-A RNA editing in chronic social defeat stress (CSDS) remains unclear. We herein identified G-to-A RNA editing and its changes in the ventral tegmental area (VTA), a key region of the brain reward system, in CSDS mouse models under emotional stress (ES) and physiological stress (PS) conditions. Our results revealed 3812 high-confidence G-to-A editing events. Among them, 56 events were significantly downregulated while 23 significantly upregulated in CSDS compared to controls. Moreover, divergent editing patterns were observed between CSDS mice under ES and PS conditions, with 42 and 21 events significantly upregulated in PS and ES, respectively. Interestingly, differential RNA editing was enriched in genes with multiple editing events. Genes differentially edited in CSDS included those genetically associated with mental or neurodevelopmental disorders, especially mood disorders, such as FAT atypical cadherin 1 and solute carrier family 6 member 1. Notably, changes of G-to-A RNA editing were also implicated in ionotropic glutamate receptors, a group of well-known targets of adenosine-to-inosine RNA editing. Such results demonstrate dynamic G-to-A RNA editing changes in the brain of CSDS mouse models, underlining its role as a potential molecular mechanism of CSDS and stress-related diseases.

Лимфоцитарный индекс периферической крови у крыс на разных стадиях постстрессорного периода в условиях антигенного воздействия при введении липополисахарида

Changes in the Shaganin lymphocyte index (ratio of the number of lymphocytes to segmented neutrophils) in the peripheral blood of rats after intraperitoneal administration of LPS (100 μg/kg) at the end of a single stress exposure in a model of 24-h restraint stress were studied. The lymphocyte index was analyzed 3 h later, on the 1st and 8th days after the stress load. Immobilization was accompanied by a decrease in this parameter 3 h after exposure. One day after the stress load, an increase in the lymphocyte index was noted, which remained on the 8th day of observation. LPS injection did not affect the changes in this parameter caused by 24-h immobilization on the 1st and 8th days of the study, but prevented a pronounced increase in the lymphocyte index on the 1st day after the stress load. The data obtained expand the existing scientific understanding of the specificity of the involvement of immunomodulatory substances in the implementation of adaptive-compensatory processes in mammals under conditions of emotional stress.

Analysis of the emotional strain influence on the indicators of the heart rhythm variability in students

To determine the features of the cardiovascular system functional state in students using some indicators of heart rate variability under emotional strain. The research involved 67 male students with an average level of daily motor activity. The age range corresponded to 18 - 23 years. All the examined in the personal history had no deviations in health status and were considered to belong to the main medical group. The tonic activity of the autonomic nervous system in the normal condition was determined using the rhythmocardiographic method on the “Varicard 2.51” hardware-software complex according to the indicators of heart rate variability mathematical analysis. Cardio intervals were recorded in the normal condition and under conditions of emotional stress (the examination session was a model of stress). The analysis of heart rate variability was carried out on the basis of the statistical analysis indicators evaluation - SDNN, pNN50 and variational pulsometry - Mo, AMo, MxDMn, IN. Depending on the initial tonicity of the autonomic nervous system, all examined students were divided into groups: vagotonics, normotonics and sympathotonics. Among the determined values, the examination stress had the greatest impact on the stress index and the share of neighboring RR intervals, which differ by more than 50 ms. If the stress index significantly increased in students with vago- and normotonia, the proportion of neighboring RR intervals, differing by more than 50 ms, decreased in all the studied groups Taking an exam led to psycho-emotional stress of the body. It caused autonomic provision restructuring of cardiac activity due to an increase in the sympathetic effect. The results of the research may be of interest to all participants of the educational process.

Interdependence of oxidative/antioxidant system indicators and thyroid status under conditions of prolonged exposure to small doses of radiation

We have studied the interdependence of the intensity of oxidative processes/antioxidant level and the thyroid status parameters in a group of students aged 18–24 who lived for a long time in the territory of enhanced radioecological control (density of soil contamination by isotopes (137Cs 3.7 ∙ 104 – 18.5 ∙ 104 Bq/m2). We examined 50 people from relatively environmentally friendly areas (control group) and 50 people from IV radiation zone (experimental group). In the experimental group, there were no individuals with clinical manifestations of thyroid pathology. However, subgroups with signs of hyperthyroidism and hypothyroidism were identified. We evaluated the level of cortisol, thyrotrophic hormone (TSH), triiodothyronine (T3), thyroxine (T4), malonic dialdehyde (MDA), ceruloplasmin (CP), transferrin (Tf), sulfhydryl groups (SH); we calculated the oxidative stress index (OSI). The research was conducted one month before the examination time and also during the exams as a factor in increased emotional stress. A lowered CP level was found in the subgroup with signs of hypothyroidism; SH groups – in all subgroups, separated by thyroid status. The oxidative stress index was higher in all students examined of the experimental group, compared with the control. The growth of MDA level is marked in the experimental group – it is the most strongly pronounced in conditions of additional emotional load in people with signs of hyperthyroidism and hypothyroidism. CP level significantly decreased in the subgroup of hyperthyroidism on the background of T3 decrease. OSI increased in all students examined from the experimental group. In the subgroup of hypothyroidism it became significantly higher than in the subgroup of euthyroidism. A positive correlation between the levels of CP and T3 was found. The highest values of the correlation coefficients were noted for subgroups with signs of hyperthyroidism and hypothyroidism, with the coefficient significance increasing under conditions of emotional stress. The index of oxidative stress in the experimental group positively correlated with the level of TSH – in terms of emotional stress, the statistical significance of the coefficients disappeared. In the subgroups divided by thyroid status, variability of interactions between OSI and T3 was observed but it was not statistically significant. It was found that the participation of thyroid status in supporting redox homeostasis in people aged 18–24 who suffered from chronic small-doze radiation exposure was realized mainly by the influence on the antioxidant system. The ability of thyroid hormones to maintain a proper antioxidant state was suppressed in this group. The unbalanced relationship between thyroid hormones and oxidative stress indicators is strongly manifested under conditions of additional emotional stress.

Effects of Intrahippocampal Injections of Melipramin on EEG, Brain Self-Stimulation, and Behavioral Indices of Rats under Conditions of Neurogenic Stress

In young (3-month-old) male Wistar rats, we studied the effects of intrahippocampal microinjections of melipramin under conditions of emotional stress induced by sessions of electrical nociceptive stimulation of the limbs. In most cases, seven-day-long stressing resulted in suppression of electrical activity of the hippocampus, exerted ambivalent effects on the frequency of brain self-stimulation (BSS) events, decreased the communicability of animals in the two-chamber test, and increased the latencies of going of the animal from the arena center and grooming episodes in the open field test. Injections of melipramin interfered with the development of stress-induced disorders in the hippocampus, decreased the normalized content of beta oscillations in the neocortex and the theta-index in the hypothalamus, increased the BSS frequency, and decreased the level of negativation of the behavioral manifestations of this phenomenon. The duration of stay of a rat near the partition in the two-chamber test increased, the mobility and number of grooming episodes in the open field enhanced, but the latency of leaving the arena center also increased (similarly to what was observed in the control). On the 8th–9th day of the poststress period, behavioral disorders of rats of the control group in the two-chamber test were smoothed to a significant extent. The stability of the examined indices or the reverse development of stress-induced disorders within the above period in animals injected with melipramin were indicative of a significant role of the hippocampus in the formation of central effects of this antidepressant and also of adaptogenic and stress-protective properties of this drug.

Specifics of adaptation capability of pediatric dentists with different psychological types in emotional stress conditions

High emotional pressure, experienced by pediatric dentists at work frequently leads to emotional exhaustion and distress. Disorders of adaptation mechanisms come out in length of time as somatic health problems. The intensity of adaptation system response significantly depends on psychological type of an exact individual. The article analyses numerical and qualitative indicators of health among pediatric dentists during their professional activity.

Effect of Xanthine Derivatives on the State of the Cardiovascular System in Conditions of Emotional Stress

Effect of Xanthine Derivatives on the State of the Cardiovascular System in Conditions of Emotional Stress

Effect of Acute Emotional Stress on Proteomic Profile of Selected Brain Areas and Lysosomal Proteolysis in Rats with Different Behavioral Activity.

We compared proteome profiles of selected brain areas (cortex, amygdala, hippocampus, and reticular formation) and measured cathepsins B and D activity in liver lysosomal fraction in rats with different behavioral activity under conditions of emotional stress. In passive rats, the expression of some proteins in various brain regions was changed and baseline cathepsin B activity was higher than in active animals. Taken together, the results attest to differences in the adaptive response formation in rats, depending on behavioral features.

Effects of sociopsychological factors on the development of occupational stress

The article presents data on social-psychological factors which cause the occupational stress. The results showed that there is a link between the level of work motivation and the physiological cost of work. We observed a number of peculiarities of occupational stress development caused by psychoemotional tension depending on the class of intensity of intellectual labor; we also studied biological age of the subjects. The speed of ageing of the employees who work under conditions of emotional stress (direct or indirect responsibility for the safety of other people) was found to increase. The study suggested promising directions of occupational stress prevention.

PP.17.23

Objective: Accurate diagnosis of haemodynamic instability is difficult in the emergency department. We need to rule out immediately the life threatening conditions which could evolve with such clinical findings but after, it could be a “long way” to the correct diagnosis. Design and method: A case report with an unexpected cause of orthostatic hypotension. Results: This is the case of a patient aged 73 with newly diagnosed uncomplicated type II diabetes, without known arterial hypertension, who presented in the emergency department with resting tachycardia, occipital headache and clinically significant orthostatic hypotension (faintness, 60mmHg decrease in SBP). In emergency we ruled out subarachnoid hemorrhage, acute hemorrhagic syndrome and pulmonary embolism by chest CT. We also ruled out acute coronary syndrome, atrioventricular or aortic obstruction by echocardiography and laboratory investigations. Orthostatic hypotension is maintained for 48 hours despite aggressive fluid reppletion, the patient relating intermittent episodes of occipital headache, nonspecific chest pain, sweating and flush occurring especially during emotional stress conditions (venipuncture) or mictionary effort. Monitoring BP/24 h reveals values rising to 220/140mmHg with spontaneous remission which rise the suspicion of pheochromocytoma. The dosage of free plasma metanefrines indicate values more than 10 times the upper limit of normal and abdominal CT identified a tumor on the right adrenal looking compatible with pheochromocytoma. Treatment with alpha and beta blockers was started and, after 2 weeks, the patient was referred to surgery. The pathological examination revealed alveolar growth pattern in anastomosed cords and in difuse, solid areas, marked cytological and nuclear pleomorphism of cells, nuclear inclusions, hipercromatism, confirming the diagnosis of pheochromocytoma. The evolution at 1 month was favorable with normalisation of blood pressure values and glycemia. Conclusions: The typical clinical presentation of pheochromocytomas is constant elevated blood pressure or normal blood pressure with significant paroxysm related to variant stimulus. However, in some undiagnosed situations with long evolution, there is an important constriction of the vascular space leading to significant orthostatic hypotension which can further hide the real diagnosis. We appreciate it is worthy to keep in mind this “edge” diagnosis after excluding the most common causes of orthostatic hypotension.

Biochemical features in rats with different behavioral activity under conditions of emotional stress.

Adaptive capacities of the organism under conditions of emotional stress are determined by individual complex of anti-stress protective mechanisms. We studied the content of coenzyme Q10 and the spectrum of polyunsaturated fatty acids omega-3 and omega-6 in the serum, liver, and brain, and LPO intensity in the serum and liver of behaviorally active and passive rats under conditions of acute emotional stress. The differences in the formation of adaptive responses in animals with various behavioral activities were demonstrated.

Genetic loci for spleen weight and blood pressure in ISIAH rats with inherited stress-induced arterial hypertension

Recently, the important role of the spleen function in hypertension development was demonstrated. In this study, the genetic control of absolute and relative spleen weight was investigated to reveal the genetic loci common for spleen traits and for arterial blood pressure at rest and under the emotional stress conditions in the ISIAH rats with inherited stress-induced arterial hypertension. The search for genetic loci for absolute and relative spleen weight was performed on 6-month old F2 (ISIAH x WAG) hybrid males derived from a cross of hypertensive ISIAH and normotensive WAG rats. One significant QTL mapped on chromosome 1 and 5 suggestive loci were found for relative spleen weight. Four suggestive loci were detected for absolute spleen weight. All detected loci were novel. The significant QTL on chromosome 1 was common for relative spleen weight and arterial blood pressure at rest and under the emotional stress conditions in ISIAH rats. The results suggest that the manifestation ofthe stress-sensitive arterial hypertension in ISIAH rats may be related to the changes in genetic control of the spleen function.

Proteomic study of rat hippocampus under conditions of emotional stress.

Proteomic differences in the hippocampus of stress-resistant and stress-sensitive rats were detected on the model of emotional stress. Differential expression of some proteins was detected in animals with different behavioral activity initially and after experimental stress exposure. Specific involvement of the hippocampus in the realization of stress response in animals with different sensitivity to emotional stress was demonstrated.

Neural changes associated with emotion processing in children experiencing peer rejection: a functional MRI study.

This study was performed to investigate differences between children who did and did not experience peer rejection in psychological state through surveys and in emotion processing during an interpersonal stress challenge task to reflect naturalistic interpersonal face-to-face relationships. A total of 20 right-handed children, 10 to 12 yr of age, completed self-rating questionnaires inquiring about peer rejection in school, depression, and anxiety. They then underwent an interpersonal stress challenge task simulating conditions of emotional stress, in reaction to positive, negative and neutral facial expression stimuli, using interpersonal feedbacks, and functional magnetic resonance imaging (FMRI) for an analysis of neural correlates during the task. Ten were the peer-rejection group, whereas the remainder were the control group. Based on the behavioral results, the peer-rejection group exhibited elevated levels of depression, state anxiety, trait anxiety and social anxiety as compared to the control group. The FMRI results revealed that the peer-rejection group exhibited greater and remarkably more extensive activation of brain regions encompassing the amygdala, orbitofrontal cortex and ventrolateral prefrontal cortex in response to negative feedback stimuli of emotional faces. The different brain reactivities characterizing emotion processing during interpersonal relationships may be present between children who do and do not experience peer rejection.Graphical

Blood Cytokines in Rats with Various Behavioral Characteristics during Emotional Stress and Treatment with Interleukin-1β

We studied the effect of acute emotional stress and exogenous IL-1beta (5 microg/kg intraperitoneally) on the cytokine profile of blood serum in Wistar rats with various behavioral characteristics in the open-field test. Blood level of proinflammatory cytokine IL-1beta decreased in behaviorally passive rats, but increased in active animals after simultaneous immobilization and electrocutaneous stimulation. These changes reflect the opposite immune responses to a similar stress exposure in rats with different emotional reactivity. Poststress variations in the concentration of circulating IL-1beta differed in rats receiving exogenous IL-1beta. Blood cytokine concentration decreased in behaviorally active rats, but remained unchanged in passive animals that were exposed to immobilization and electrocutaneous stimulation after pretreatment with IL-1beta. Emotional stress and injection of IL-1beta had no effect on blood level of an anti-inflammatory cytokine IL-4 in rats. Our results indicate that rats with various behavioral parameters are characterized by significant differences in the cytokine profile of blood serum under conditions of emotional stress and treatment with IL-1beta. These data illustrate the specific functional features of immune mechanisms, which provide an individual resistance of rats to the same stress exposure.

Pharmacological activity and clinical effects of trecrezan and other tris-(2-hydroxyethyl)ammonium salts of arylheteroacetic acids (A review)

The results of an experimental and clinical study on the pharmacological properties of new compounds representing tris-(2-hydroxyethyl)ammonium salts (TES) of arylheteroacetic acids-in particular, tris-(2-hydroxyethyl)ammonium-2-methylphenoxyacetate (trecrezan)-are summarized. Trecrezan exhibits a modulating action on immunity and hemopoiesis and offers a promising approach to immune correction for the treatment of immune deficiency and autoimmune disorders. Recovery of the proliferative activity of the early precursors of erythropoiesis with the aid of trecrezan (experimental model) leads to the normalization of immunity and hemopoiesis. A method involving trecrezan administration has been developed for the complex treatment of acute viral hepatitis B as well as hepatitis A and B complicated by occupational anamnesis. Trecrezan has also been successfully applied for the complex treatment of ischemic heart disease and multiple sclerosis. Trecrezan stimulates the regeneration of hepatocytes during partial hepatectomy and acts as an effective adjuvant in the complex therapy of tuberculosis. Trecrezan analogs (TES) show antitumor, hepatoprotective, and adaptive activity, increase the resistance of animals to oxygen insufficiency and to poisoning with heavy metal salts and organophosphorus compounds, and improve the endurance of animals under physical load and emotional stress conditions.