To compare the accommodation response (AR) to monochromatic targets in subjects with different color vision statuses, and to investigate the role of color vision in the control of accommodation and emmetropization. It was a case-control study. Accommodation was measured with a dynamic infrared optometer while subjects [17 protans, 47 deutans, and 23 normals; mean age: (20.0 ± 4.4) years] viewed a (1) red on black or (2) green on black vertical square-wave gratings of iso-luminance (3 cycles/deg; 0.9 contrast) in a Badal optic system. The grating stepped 1.00 D towards the eye from an initial position of 0 D until 5.00 D. With red-black targets, the AR in the protans (AR = 1.98 D) was worse than that in the normals (AR = 2.55 D) when the accommodation stimulus (AS) was 4.00 D (LSD, P = 0.031). The AR in the deutans were worse than that in the normals when the AS was 3.00, 4.00, and 5.00 D (3.00 D: 1.23 D vs. 1.69 D, P = 0.002; 4.00 D: 1.89 D vs. 2.55 D, P = 0.002; 5.00 D: 2.40 D vs. 3.17 D, P = 0.003). With green-black targets, the AR in the protans were worse than that in the normals when the AS was 3.00 and 4.00 D (3.00 D: 1.13 D vs. 1.61 D, P = 0.004; 4.00 D: 1.80 D vs. 2.34 D, P = 0.021). In the deutans, the AR was worse with stimuli of 3.00, 4.00, and 5.00 D (3.00 D: 1.21 D vs. 1.61 D, P = 0.003; 4.00 D: 1.65 D vs. 2.34 D, P < 0.001; 5.00 D: 2.36 D vs. 2.93 D, P = 0.007). No significant differences between the protans and deutans were found for all the stimulus conditions. In the protans, accommodation to red-black targets was better than that to green-black targets when the stimulus was 2.00, 3.00, and 5.00 D (2.00 D: t = -2.81, P = 0.013; 3.00 D: t = -4.55, P < 0.001; 5.00 D: t = -3.15, P = 0.006). In the deutans, accommodation to red-black targets was better than that to green-black targets when the stimulus was 4.00 D (t = -2.19, P = 0.034). In the normals, accommodation to red-black targets were better than that to green-black targets when the stimulus was 2.00, 4.00, and 5.00 D (2.00 D: t = -2.57, P = 0.017; 4.00 D, t = -2.67, P = 0.014; 5.00 D: t = -2.15, P = 0.043). Individuals with a color vision deficiency tend to have a larger accommodative lag than normals. Red targets tend to induce better accommodation response than green ones. Color vision may play a role in the control of accommodation and emmetropization.