During computer work in controlled laboratory conditions, wearing multifocal contact lenses (MFCLs) showed no lower muscle load but increased subjective perception of comfort with equivalent visual quality and comparable tolerance. Because musculoskeletal complaints are frequent among computer workers, this study used the muscle electrophysiological activity of shoulder and neck muscles in presbyopic computer workers who received either progressive addition lenses for general purpose (GP-PALs) or MFCLs. For this crossover study, 11 presbyopic computer workers aged 55 ± 4 years (mean ± standard deviation) were equipped with GP-PALs and MFCLs in a randomized order. Surface electromyography signals were recorded bilaterally from shoulder and neck muscles during short-term computer work tasks using an optimally adjusted visual display unit workplace. The amplitude probability distribution function, the number and total duration of EMG gaps, and sustained low-level muscle activity periods of the surface electromyography signals were calculated. Comfort and correction type preferences were assessed. Head inclination was objectively evaluated. Multifocal contact lenses elicited no significant lower muscle load than GP-PALs. The number of sustained low-level muscle activity periods longer than 60 seconds was similar between visual aids. The total amount of gaps was significantly higher with MFCLs (44 gaps) compared with progressive addition lenses for general purpose (15 gaps) in all analyzed periods for all participants. However, there were no significant differences for the median in the intraindividual comparisons (P = .22, dz = 0.52). Multifocal contact lenses scored statistically significant higher in comfort values with equivalent visual quality and comparable tolerance (P = .003, dz = 1.51). Although the study failed to show clear results, wearing MFCLs seems to enhance working comfort compared with GP-PALs subjectively.