Emergent Pollutant Research Articles

The European DeuteroNoise project: impact of anthropogenic noise on marine invertebrate deuterostomes

Underwater noise is considered an emergent pollutant negatively affecting marine fauna; however, its effects on marine invertebrates are almost unknown. In the frame of the project DeuteroNoise, financed by JPI Oceans, we tested the effects of anthropogenic underwater noise on the behavioral performances and physiology of Deuterostome species. Experimental designs for noise exposure in the laboratory were performed inside tanks and directed toward each species studied. For all species, the playback experiments were performed inside water tanks using an underwater speaker connected to an amplifier and a computer. The animals were exposed to pink noise (higher intensities at 63-125 Hz) at different intensities. Behavioral and physiological responses varied among species depending on the anatomy of their sensitive system and the studied response (e.g. movement, development, heartbeat).

Characteristics of microplastics and their abundance impacts on microbial structure and function in agricultural soils of remote areas in west China

As an emergent pollutant, microplastics (MPs) are becoming prevalent in the soil environment. However, the characteristics of MPs and the response of microbial communities to the abundance of MPs in agricultural soils in West China still need to be elucidated in detail. This study utilized the Agilent 8700 Laser Direct Infrared (LDIR) to analyze the characteristics of small-sized MPs (20–1000 μm) in soils from un-mulched and mulched agricultural fields in West China, and illustrated their correlation with microbial diversity. The results revealed a higher abundance of MPs in mulched soil ((4.12 ± 2.13) × 105 items kg−1) than that in un-mulched soil ((1.04 ± 0.26) × 105 items kg−1). The detected MPs were dominated by fragments, 20–50 μm and Polyamide (PA). High-throughput sequencing analysis indicated that alpha diversity (Chao1 and Shannon indices) in the plastisphere was lower compared to that in soil, and varied significantly with MPs abundance in soil. As the abundance of MPs increased, the proportion of soil about the degradation of organic matte and photoautotrophic taxa increased, which showed enrichment in the plastisphere. Functional predictions further indicated that MPs abundance affected potential soil functions, such as metabolic pathways associated with the C and N cycling. The plastisphere showed higher functional abundance associated with organic matter degradation, indicating higher potential health risks compared to soil environments. Based on the RDA analyses, it was determined that environmental physicochemical properties and MPs abundance had a greater impact on fungal communities than on bacterial communities. In general, the abundance of MPs affected the microbial diversity composition and potentially influenced the overall performance of soil ecosystems. This study offers empirical data on the abundance of MPs in long-term mulched agricultural fields and new insights for exploring the ecological risk issues associated with MPs.

Seawater quality criteria derivation and ecological risk assessment for dichlorvos in China

Dichlorvos (DDVP) is a widely used organophosphorus pesticide (OPP) that has been frequently detected in the marine environment of China. Water quality criteria (WQC) is however not available for this emergent pollutant in the marine environment, which hinders its ecological risk assessment. This study, therefore, screened toxicity values of DDVP and conducted toxicity tests on six marine species to supplement toxicity data. The WQC for DDVP was derived with the species sensitivity distribution (SSD) methodology, based on which the ecological risk of DDVP in the seawater of China was assessed. The results showed that the recommended short-term (SWQC) and long-term water quality criteria (LWQC) for DDVP were 1.47 and 0.0521 μg/L, respectively. Most marine waters of China showed low or negligible risk (HQ < 1, ORP < 2 %), whereas some estuarine waters warrant further concern due to higher risk. This study provides the scientific basis for seawater quality standard formulation and ecological risk management for DDVP.

Laccase-based catalytic microreactor for BPA biotransformation

A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.

The Impacts of the Multispecies Approach to Caffeine on Marine Invertebrates.

Caffeine is one of the most consumed substances by humans through foodstuffs (coffee, tea, drugs, etc.). Its human consumption releases a high quantity of caffeine into the hydrological network. Thus, caffeine is now considered an emergent pollutant sometimes found at high concentrations in oceans and seas. Surprisingly, little research has been conducted on the molecular responses induced by caffeine in marine organisms. We studied, in laboratory conditions, six phylogenetically distant species that perform distinct ecological functions (Actinia equina and Aulactinia verrucosa (cnidarians, predator), Littorina littorea (gastropod, grazer), Magallana gigas (bivalve, filter-feeder), and Carcinus maenas and Pachygrapsus marmoratus (crabs, predator and scavenger)) subjected to caffeine exposure. The antioxidant responses (catalase, CAT; glutathione peroxidase, GPx; superoxide dismutase, SOD), lipid peroxidation (MDA), and the acetylcholinesterase (AChE) activity were estimated when the organisms were exposed to environmental caffeine concentrations (5 μg/L (low), 10 μg/L (high)) over 14 days. Differential levels of responses and caffeine effects were noted in the marine invertebrates, probably in relation to their capacity to metabolization the pollutant. Surprisingly, the filter feeder (M. gigas, oyster) did not show enzymatic responses or lipid peroxidation for the two caffeine concentrations tested. The marine gastropod (grazer) appeared to be more impacted by caffeine, with an increase in activities for all antioxidative enzymes (CAT, GPx, SOD). In parallel, the two cnidarians and two crabs were less affected by the caffeine contaminations. However, caffeine was revealed as a neurotoxic agent to all species studied, inducing high inhibition of AChE activity. This study provides new insights into the sublethal impacts of caffeine at environmentally relevant concentrations in marine invertebrates.

Photo-Chemical Sucralose Degradation in an Aqueous Medium Using a Photo-Fenton System Equipped with Stainless Steel Mesh Cathodes Modified By Nanostructured TiO2- and C|TiO2-Based Films for Continuous Electro-Generation of H2O2

Artificial non-caloric sweeteners are non-bioassimilable substances that pass through the human body without biochemical changes. Sucralose, for instance, is 600 times sweeter than natural sucrose, so it is widely used in beverages and processed food products without regulation in many countries. Its environmental persistence of 337 days in sludge allows inferring that these substances can adversely affect the environment in the short or long term, thus classifying sucralose as an emergent pollutant. In this work, stainless steel (SS) mesh working electrodes were modified by TiO2- or C|TiO2-based nanoparticulate films (where C is carbon Vulcan). Both types of electrodes were employed as cathodes for the electro-generation of hydrogen peroxide (H2O2), which was the chemical precursor for photochemical producing •OH radicals able for sucralose degradation in a photo-Fenton system equipped with a 254 nm light source. Preparation of TiO2- and C|TiO2-based cathodes was performed by electrophoretic deposition method (EPD, 2 V/cm for 40 s) on AISI 304 SS mesh, followed by sintering at 450°C for 1h in the air. The EPD process was performed using aqueous colloidal suspensions containing wt./wt. ratios of C/TiO2 = 1/100 and 1/10, or TiO2 without C for comparison purposes. H2O2 electro-generation was carried out via Reaction 1 utilizing a two-electrode cell (3V-cell polarization) containing sucralose dissolved in a pH 2 aqueous sulfates buffer solution perpetually bubbled by air (volumetric flow rate of 17 mL/s) containing gaseous O2. In all the cases, a Ti grade 2.0 rod anode was immersed in the electrolyte medium with one of the following cathodes: SS* (polished, degreased, and heated at 450°C for 1h in the air as control electrode), SS||TiO2 and SS||C|TiO2 (wt./wt. ratios of C/TiO2 = 1/100 or 1/10). Furthermore, the cell was illuminated by a 254 nm lamp in order to continuous promotion of the photo-chemical Reaction 2.O2 + 2H+ + 2e- → H2O2 ...............(1)H2O2 254mn→ 2°OH...................... (2)Sucralose degradation efficiencies were registered based on the cathodes employed for continuous H2O2 electro-generation. Furthermore, the relative use time was also registered for each cathode. A comparison of these results reveals that when employed SS*, SS||TiO2, and SS||C|TiO2 (wt./wt. ratio of C/TiO2=1/100) cathodes the sucralose degradation achieved efficiencies over 90% (i.e. 99.1, 96.8, and 91.3%, respectively). In contrast, SS||C|TiO2 (wt./wt. ratio of C/TiO2=1/10) cathodes showed a sucralose degradation efficiency of only 88.6%. Furthermore, the SS||TiO2 and SS||C|TiO2 cathodes showed durability 3 times higher than for the SS* cathodes, and 1.8 times higher than for SS||C|TiO2 (wt./wt. ratio of C/TiO2=1/10). These observations indicated that both, SS||TiO2 and SS||C|TiO2 cathodes are strong candidates for assembling more efficient photo-Fenton systems for the degradation of sucralose and other artificial sweeteners.

First basin scale spatial–temporal characterization of underwater sound in the Mediterranean Sea

Anthropogenic underwater noise is an emergent pollutant. Despite several worldwide monitoring programs, only few data are available for the Mediterranean Sea, one of the global biodiversity hotspots. The results of the first continuous acoustic programme run at a transnational basin scale in the Mediterranean Sea are here presented. Recordings were done from March 2020 to June 2021, including the COVID-19 lockdown, at nine stations in the Northern Adriatic Sea. Spatial–temporal variations of the underwater sound are described, having one third octave band sound pressure levels (SPLs) from 10 Hz to 20 kHz as metrics. Higher and more variable SPLs, mainly related to vessel traffic, were found close to harbours, whereas Natura 2000 stations experienced lower SPLs. Lower values were recorded during the lockdown in five stations. Median yearly SPLs ranged between 64 and 95 as well as 70 and 100 dB re 1 µPa for 63 and 125 Hz bands, respectively. These values are comparable with those previously found in busy shallow EU basins but higher levels are expected during a business-as-usual period. This is a baseline assessment for a highly impacted and environmental valuable area, that needs to be managed in a new sustainable blue growth strategy.

Co-doped metal–organic framework MIL-125(Ti) derivate for efficient adsorption of tetracycline hydrochloride from water

Metal-organic framework (MOF) based materials have aroused much research interest owing to their unique structural features in the field of environmental purification. Herein, a MOF derivate of Co-MIL-300 was prepared by sintering the cobalt-modified MIL-125(Ti) (Co-MIL) precursor at 300 °C in an air atmosphere and used as adsorbent to remove tetracycline hydrochloride (TCH, a typical emergent pollutant) from water. The Co-MIL-300 adsorbent possesses a large specific surface area, the rich surface oxygen-containing groups, the benzene-ring structure with rich π-electrons and surface cobalt ions benefiting for TCH adsorption. The TCH adsorption on Co-MIL-300 satisfies the pseudo-second-order kinetic model and Langmuir mode well, and is found to be a spontaneous endothermic and monolayer chemical adsorption process based on covalent bonding (such as π-π conjugation interactions, hydrogen bonding, π-cation interactions, coordination bonding) adsorption mechanism. Co-MIL-300 reveals a much higher adsorption capacity than Co-MIL (2.3 times of the latter) with qmax = 321.5 mg⋅g−1 at 30 °C, and it also keeps a high adsorption capacity in a wide pH range (pH = 2–11). The coexisting CO32–, PO43-, Al3+ and Fe3+ ions can greatly inhibit TCH adsorption. Most of its adsorption capacity can be recovered in five cycles after regeneration, showing great application potential for antibiotics removal.

Size-selective microplastic uptake by freshwater organisms: Fish, mussel, and zooplankton

Microplastics, as an emergent pollutant, have garnered substantial attention within aquatic environments, yet a significant knowledge gap persists regarding the interplay of organism size and pollution impacts on microplastic uptake in freshwater ecosystems. The main aim of the current study is to assess the microplastic ingestion by aquatic organisms across diverse trophic levels. To achieve this objective, zooplankton, mussels (Anodonta anatina), and fish (Carassius gibelio) were collected from the highly polluted Susurluk River Basin in Türkiye. The size distribution encompassed 160.8 ± 56.9 μm for the prevailing zooplankton, 6.9 ± 2.2 cm for mussel, and 20.4 ± 3.1 cm for fish, respectively. While no microplastic ingestion was observed among zooplankton, the finding highlights the influence of body-size and pollution on microplastic ingestion. In contrast, A. anatina and C. gibelio contained 617 and 792 microplastic particles, respectively. Predominantly, fibers emerged as the most prevalent microplastic type across trophic levels (except zooplankton) followed by films. Notably, only fish exhibited fragments within their gastrointestinal tract. A substantial correlation emerged between microplastic abundance and mussel size and weight, but no such correlation manifested for fish. The study also revealed a positive link between microplastic count and turbidity (phosphate and high Chl a level), impacting mussel ingestion capacity due to the variability in the food availability and potential shifts in feeding preferences. Conversely, no distinct pattern emerged for fish concerning water quality parameters and ingested microplastics. Consequently, our study underscores diverse microplastic uptake patterns in freshwater ecosystems, with a predominant frequency of microplastics falling with the 0.3 mm–3.0 mm range, emphasizing the significance of size-selective uptake by organisms.

Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways.

(1) Background: Acetaminophen (APAP), an active component of many analgesic and antipyretic drugs, is one of the most concerning trace contaminants in the environment and is considered as an emergent pollutant of marine and aquatic ecosystems. Despite its biodegradability, APAP has become a recalcitrant compound due to the growth of the global population, the ease of availability, and the inefficient wastewater treatment applied. (2) Methods: In this study, we used a transcriptomic approach to obtain functional and metabolic insights about the metabolization of APAP by a phenol-degrading fungal strain, Penicillium chrysogenum var. halophenolicum. (3) Results: We determined that the transcriptomic profile exhibited by the fungal strain during APAP degradation was very dynamic, being characterized by an abundance of dysregulated transcripts which were proportional to the drug metabolization. Using a systems biology approach, we also inferred the protein functional interaction networks that could be related to APAP degradation. We proposed the involvement of intracellular and extracellular enzymes, such as amidases, cytochrome P450, laccases, and extradiol-dioxygenases, among others. (4) Conclusions: Our data suggested that the fungus could metabolize APAP via a complex metabolic pathway, generating nontoxic metabolites, which demonstrated its potential in the bioremediation of this drug.

The relative size of microalgal cells and microplastics determines the toxicity of microplastics to microalgae

Microplastics (MPs), as an emergent pollutant, are causing global attention in terms of water pollution, which poses a great detriment to aquatic organisms, like algae, fish and shellfish. This study aimed to investigate the toxicity of MPs of different particle sizes to microalgae with different cell diameters. Here, we conducted a microcosm batch experiment to investigate the toxicity of four particle sizes (0.2 µm, 0.5 µm, 1 µm and 5 µm) of polystyrene MPs (PS-MPs) to 12 species of microalgae. Our results found that PS-MPs of 0.5 and 1 µm had the highest inhibition rate on the microalgae. By classifying 12 species of microalgae into small, medium and large particles sizes, the inhibition of PS-MPs on algal growth gradually increased with larger cells, indicating that they were more susceptible to inhibition. The inhibition rate of PS-MPs on three phyla was Chlorophyta < Cyanobacteria < Bacillariophyta. The contents of reactive oxygen species and malondialdehyde in microalgae cells increased under PS-MPs exposure, suggesting that PS-MPs caused damage to the antioxidant system of microalgae. Together, these findings identified that the toxicity of MPs to microalgae was correlated with the relative size of each other, contributing to evaluate the risk of MPs for aquatic ecosystems.

Degradation of ciprofloxacin by green cobalt oxide quantum dots

Ciprofloxacin (CIPRO) is a fluoroquinolone antibiotic extensively used to treat various bacterial infections. The extensive use of CIPRO has promoted its accumulation in diverse aquatic environments as an emergent pollutant. Concentrations of CIPRO found in surface and ground waters and sea vary from ng to mg/L and represent a real risk for the rise of new resistant bacterial strains. The complex and stable chemical CIPRO structure is not favorable for biological degradation. However, magnetic Co3O4 quantum dots (Co3O4 QDs) with 5 nm of mean diameter synthesized using biomolecules from tomato (Solanum lycopersicum L.) seed extract were efficient in promoting photodegradation of this emergent pollutant under UV irradiation. UV–visible and Fourier Transform Infrared (FTIR) spectrophotometry corroborated CIPRO degradation. The photodegradation using UV light in the presence of Co3O4 QDs was the more efficient strategy to impair the bactericidal action of CIPRO and, consequently, avoid the development of bacteria-resistant strains. The Co3O4 QDs are a low-cost, efficient, and green option for CIPRO degradation.

Pollutants corrupt resilience pathways of aging in the nematode C. elegans

SummaryDelaying aging while prolonging health and lifespan is a major goal in aging research. One promising strategy is to focus on reducing negative interventions such as pollution and their accelerating effect on age-related degeneration and disease. Here, we used the short-lived model organism C. elegans to analyze whether two candidate pollutants corrupt general aging pathways. We show that the emergent pollutant silica nanoparticles (NPs) and the classic xenobiotic inorganic mercury reduce lifespan and cause a premature protein aggregation phenotype. Comparative mass spectrometry revealed that increased insolubility of proteins with important functions in proteostasis is a shared phenotype of intrinsic- and pollution-induced aging supporting the hypothesis that proteostasis is a central resilience pathway controlling lifespan and aging. The presented data demonstrate that pollutants corrupt intrinsic aging pathways. Reducing pollution is, therefore, an important step to increasing healthy aging and prolonging life expectancies on a population level in humans and animals.

Micro and Macroplastics Analysis in the Digestive Tract of a Sea Cucumber (Holothuriidae, Holothuria floridana) of the Placencia Lagoon, Belize

Microplastics have been identified as an emergent pollutant in the aquatic environment. Ingestion of microplastics in the wild by various biota has been documented around the globe, but little has been documented on sea cucumbers and none on microplastics in sea cucumbers in the Caribbean region. In this study, we document the presence of microplastics in the gastrointestinal tract of the sea cucumber Holothuria floridana collected from the Placencia Lagoon, Belize, Central America. The collection site is surrounded by an active tourist and fishing community that was observed to discharge solid waste directly and indirectly into the lagoon habitats of H. floridana. The results confirms the ingestion of microplastics by H. floridana. From 30 individuals averaging 22.5 cm in length, a total of 252 microplastic particles were extracted, isolated, and categorized. The mean number of microplastic particles per individual sea cucumber was 8.4 ±4.5, with a mean length of 0.703 mm ±0.706. The microplastics identified were plastic fragments (93%) and fibers (7%); with no evidence of plastic pellets (0%). This finding raises concerns regarding the chemical effects these plastics can have on human health, especially since sea cucumber intestines are consumed as luxury foods in Asian countries.

Potential of Nb2O5 nanofibers in photocatalytic degradation of organic pollutants.

Various photocatalytic nanomaterials for environmental remediation have been promoted due to the pollution caused by different organic pollutants. In this study, Nb2O5 nanofibers were obtained by electrospinning technique, presenting controlled crystallinity and high specific surface area to improve the photoactivity response. The structural characterization indicated Nb2O5 nanofibers with orthorhombic phase formation. The photoluminescence measurements showed different energy levels contributing to the electronic transition events. The nanofibers with a bandgap up to 3.6 eV were applied to photocatalysis of dyes (rhodamine B (RhB) or methylene blue (MB)) and fluoxetine (FLX), listed as an emergent pollutant. In the optimized condition (pH = 9), the RhB and MB photocatalysis was 59% and 93% more efficient than photolysis due to ζ = - 50 mV ± 5 for EtOH_550 sample increased interaction with MB (cationic) compared to RhB unprotonated (pKa = 3.7). Therefore, FLX (pKa = 10.7) was selected due to protonated form at pH = 9 and showed 68% ± 1 adsorption in 30 min for EtOH_550. The FLX photocatalytic degradation under UV light irradiation was up to 17% higher than the photolytic degradation. The formation of hydroxyl radicals in the photocatalytic system (EtOH_550) was proven by the Coumarine probe assay, corroborating with the greater amount of α-[2-(methylamino)ethyl]benzylalcohol (MAEB), a by-product obtained after FLX oxidation. Additionally, the material achieved specific catalytic activity for the different organic compounds (RhB, MB, or FLX). Therefore, Nb2O5 nanofibers were efficient for degrading three different pollutants under UV light, proving a viable alternative for environmental remediation.

Nanosilver and Silver Nitrate Toxicity in Ex Vivo-Exposed Gills of Fish and Mitigation by Humic Acids.

Silver nanoparticles (AgNP) are unique because of their biocide properties. Once released to environment, AgNP interact with the natural organic matter which impact on their fate, dispersion, and ultimate toxicity. We carried out an ex vivo exposure of gill of Corydoras paleatus fish to 100µg L-1 of AgNP or AgNO3, alone and in combination with 10mg L-1 of humic acids (HA), with the aim to evaluate the potential mitigation of HA on AgNP toxic effects. We analyzed Ag accumulation and oxidative stress biomarkers. The results showed high bioaccumulation after the AgNO3+HA exposure. An inhibition of glutathione-S-transferase enzymatic activity and depletion of reduced glutathione levels were registered after the AgNO3 exposure, and increased lipid peroxidation levels in the case of AgNP one. Oxidative responses were mitigated when the HA were present in the media. Overall, the knowledge about the fate of this emergent pollutant was deepened through this study.

Short-term ingestion and tissue incorporation of Polystyrene microplastic in the scleractinian coral Porites porites

Microplastics (MP), plastic fragments whose diameter is less than 5 mm, are an emergent pollutant in marine environments. Benthic organisms, such as corals, are at high risk of suffering toxic effects due to MP exposure. This study aims to evaluate the incorporation of polystyrene microparticles (PSm) on the reef building coral species Porites porites. PSm were synthesized employing the simple emulsion and solvent evaporation technique, obtaining round particles with a diameter of ≤5μm. P. porites fragments were exposed to four different PSm concentrations (1, 10, 100, 1000) mg/L over a period of 96 h. Histological techniques were used to evaluate PSm incorporation. No mortality was recorded during the bioassay. PSm incorporation in the gastrovascular cavity was evidenced in all of the tested concentrations. Additionally, in the highest PSm concentration (1000 mg/L), clusters were detected inside the gastrovascular tissue and mesenterial filaments. As complementary information, no statistically significant differences were found across PSm concentrations for catalase enzymatic activity and melanin content (PERMANOVA based on Euclidean distances) as a proxy of general exposure effects. This study found that P. porites is capable of ingesting microplastics, specifically polystyrene microspheres regardless of the tested concentrations, without presenting apparent toxic effects in a 96-hour exposure period. Moreover, it is shown that PSm can be incorporated inside the coral tissue. Further studies are needed to assess the potential toxic effects of MP ingestion with longer exposure times, as well as the possible mechanisms involved in MP recognition and incorporation.

Mitigation of silver nanoparticle toxicity by humic acids in gills of Piaractus mesopotamicus fish.

Silver nanoparticles (AgNPs) are one of the most produced nanoproducts due to their unique biocide properties. The natural organic matter has an important impact on nanoparticle's dispersion as it may alter their fate and transport, as well as their bioavailability and toxicity. Therefore, this study aimed to evaluate the mitigatory effect of humic acids (HAs) on AgNP toxicity. For this purpose, we carried out an ex vivo exposure of gill of Piaractus mesopotamicus fish to 100 μg L-1 of AgNPs or AgNO3, alone and in combination with 10 mg L-1 of HAs. In parallel, a complete AgNP characterization in the media, including the presence of HAs, was provided, and the Ag+ release was measured. We analyzed Ag bioaccumulation, antioxidant enzymes activities, lipid peroxidation, antioxidant capacity against peroxyl radicals, and reduced glutathione levels in fish tissue. Our results indicated the Ag+ release from AgNPs decreased 28% when the HAs were present in the media. The Ag accumulation in gill tissue exposed to AgNPs alone was higher than the AgNO3 exposure, and sixfold higher than the treatment with the HA addition. Moreover, after both Ag forms, the catalase enzyme augmented its activity. However, those responses were mitigated when the HAs were present in the media. Then, our results suggested the mitigation by HAs under the exposure to both Ag forms, providing valuable information about the fate and behavior of this emergent pollutant.

Triclosan induces immunosuppression and reduces survivability of striped catfish Pangasianodon hypophthalmus during the challenge to a fish pathogenic bacterium Edwardsiella tarda

Toxicological studies on the emergent pollutant, triclosan (TCS) have established the wide-ranging effects of the compound on fish and other aquatic organisms. Although the available literature describes the standalone effects of TCS on growth and metabolism of fish yet, reports about the combined effects of TCS with microbial pathogens are scarce. In a real environment, a combined exposure to TCS and pathogens is of common occurrence, therefore, such investigation facilitates in developing a better understanding about the gross effects of pollutants and microbial pathogens on aquatic organisms including fish. In this context, the experimental fish (striped catfish, Pangasianodon hypophthalmus) were exposed to three different concentrations of TCS viz. 10, 20 and 30% of 96 h LC50 (1177 μg L−1) for 45 days including two control group firstly solvent control (without TCS) group and another one (without solvent and TCS) group in triplicate. Sampling was performed fortnightly and blood, serum and tissues (liver, and gills) samples were collected for evaluating immunological and biochemical parameters. Following 45 days of the experiments, the experimental fish in each treatment group including controls were challenged with a fish pathogenic bacterium Edwardsiella tarda (LD50 dose) and fish mortality was daily monitored for calculating cumulative mortality till 7 days and further, relative per cent survivable was estimated. A significant reduction in cellular immune responses i.e. respiratory burst activity (RBA), myeloperoxidase activity (MPO), phagocytic activity (PA) and humoral immune components viz. serum lysozyme activity, total immunoglobulin in serum, ceruloplasmin level, serum total protein, albumin and globulin level was evident in TCS exposed groups in comparison to control during the experimental periods. Further, oxidative stress parameters viz. superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST) activity in liver and gill tissue exhibited a dose-dependent increase in activity with related to TCS concentration during the experimental periods. A significant reduction in relative percentage survival was observed with increasing TCS concentration. The present study reveals that TCS can inhibit the cellular and humoral components of the innate immune system of the fish and can elevate the mortality due to TCS mediated immunosuppression in fish during the bacterial infection.

Amino hydroxyapatite/chitosan hybrids reticulated with glutaraldehyde at different pH values and their use for diclofenac removal

Diclofenac sodium (DS) is an emergent pollutant, and among the methods investigated for its removal, adsorption is the most widely utilized technique. Hydroxyapatite and chitosan are biomaterials often used for adsorption. However, both biomaterials are limited due to their low chemical stability in an acidic medium; furthermore, pure hydroxyapatite interacts poorly with diclofenac. In this work, hydroxyapatite was organofunctionalized with 3-aminopropyltrimethoxysilane and further used to obtain amino hydroxyapatite /chitosan hybrids by crosslinking with glutaraldehyde at pH 3, 4, 5, and 6. X-ray diffraction patterns indicated the preservation of the hydroxyapatite phase under all pH conditions. Based on the control reaction of the amino hydroxyapatite with glutaraldehyde and its further reduction in sodium borohydride, the formation of CN moieties was highlighted as the main interaction mechanism between the aldehyde and amino groups. Therefore, crosslinking with glutaraldehyde was evaluated by infrared, Raman spectroscopy, and 13C NMR techniques; the results suggested contributions of imine formation and hydrogen bonding. The hybrid obtained at pH 3 exhibited an enhanced adsorption capacity of 125 mg g−1 at 15 min. The synergy between amino hydroxyapatite and chitosan crosslinked by glutaraldehyde was demonstrated.