Emergence Of Viral Diseases Research Articles

A Chromosome-level genome assembly of giant river prawn (Macrobrachium rosenbergii)

The giant river prawn (Macrobrachium rosenbergii) is one of the most widely cultured crustacean species. In recent years, its aquaculture has faced challenges, including the degradation of germplasm resources and the emergence of viral diseases. Genomic information can be a valuable resource for developing molecular breeding programs for this important aquaculture species. Here we constructed a high-quality reference genome for M. rosenbergii by integrating Nanopore, Illumina, and high-throughput chromosome conformation capture (Hi-C) technologies. The final genome assembly is 3.18 Gb in size, with scaffold N50 and contig N50 of 62.73 Mb and 8.92 Mb, respectively. Approximately 98.6% of the assembled sequences were anchored into 59 pseudo-chromosomes. Benchmarking Universal Single-Copy Orthologs (BUSCO) benchmark of the genome assembly reached 94.5%. Repetitive sequences comprise 43.77% of the assembled genome, and 17,436 protein-coding genes were annotated. The high-quality genome of M. rosenbergii will empower molecular breeding efforts and provide invaluable resources for comparative genomic analysis of decapod species.

Unveiling the Antiviral Efficacy of Forskolin: A Multifaceted In Vitro and In Silico Approach.

Coleus forskohlii (Willd.) Briq. is a medicinal herb of the Lamiaceae family. It is native to India and widely present in the tropical and sub-tropical regions of Egypt, China, Ethiopia, and Pakistan. The roots of C. forskohlii are edible, rich with pharmaceutically bioactive compounds, and traditionally reported to treat a variety of diseases, including inflammation, respiratory disorders, obesity, and viral ailments. Notably, the emergence of viral diseases is expected to quickly spread; consequently, these data impose a need for various approaches to develop broad active therapeutics for utilization in the management of future viral infectious outbreaks. In this study, the naturally occurring labdane diterpenoid derivative, Forskolin, was obtained from Coleus forskohlii. Additionally, we evaluated the antiviral potential of Forskolin towards three viruses, namely the herpes simplex viruses 1 and 2 (HSV-1 and HSV-2), hepatitis A virus (HAV), and coxsackievirus B4 (COX-B4). We observed that Forskolin displayed antiviral activity against HAV, COX-B4, HSV-1, and HSV-2 with IC50 values of 62.9, 73.1, 99.0, and 106.0 μg/mL, respectively. Furthermore, we explored the Forskolin's potential antiviral target using PharmMapper, a pharmacophore-based virtual screening platform. Forskolin's modeled structure was analyzed to identify potential protein targets linked to its antiviral activity, with results ranked based on Fit scores. Cathepsin L (PDB ID: 3BC3) emerged as a top-scoring hit, prompting further exploration through molecular docking and MD simulations. Our analysis revealed that Forskolin's binding mode within Cathepsin L's active site, characterized by stable hydrogen bonding and hydrophobic interactions, mirrors that of a co-crystallized inhibitor. These findings, supported by consistent RMSD profiles and similar binding free energies, suggest Forskolin's potential in inhibiting Cathepsin L, highlighting its promise as an antiviral agent.

A hybrid ANN-CNN model for predicting non-linear relationship of covid-19 cases based on weather factors

With the global increase in the emergence of viral diseases, the most recent being the Coronavirus Disease 2019 (COVID- 19) in 2020-2021, it has decimated the world with little understanding of its history and the factors that influence its transmission dynamics. Weather significantly influences the spread of respiratory infectious diseases like influenza, yet the impact of weather on COVID-19 transmission in Nigeria remains unexamined and necessitates further clarification. This study presents and compares the results of six machine learning models, the developed Hybrid ANN-CNN, ANN, CNN, LSTM, LASSO, and Multiple Linear Regression models, aiming to predict the impact of weather factors on COVID-19 cases. The dataset used in this study includes daily datasets of Nigerian COVID-19 cases and seven weather variables collected from May 1, 2020, to April 30, 2021. The results indicate that the developed Hybrid ANN-CNN outperforms the remaining five models based on Mean Absolute Error (MAE) and Root Mean Squared Error (RMSE) for all cases. Specifically, for confirmed cases, the Hybrid ANN-CNN had an MAE of 0.0274, for recovery cases 0.0257, and for death cases 0.0425. Similarly, for RMSE, the developed Hybrid ANN-CNN had values of 0.0469 for confirmed cases, 0.0813 for recovery cases, and 0.0840 for deaths. This was followed by LASSO with an MAE of 0.01384 and CNN and LSTM with 0.1384 and 0.1385, respectively.

New emerging viral disease on Giant Gourami (Osphronemus goramy Lac.) in Java, Indonesia

This study investigates the role of pathogens in the disease outbreaks and mass mortality affecting giant gourami (Osphronemus goramy) populations from 2018 to 2020, leading to significant economic losses. Presumptive diagnoses, based on clinical symptoms and references, implicated two viruses: Tilapia Lake Virus (TiLV) and Infectious Spleen and Kidney Necrosis Virus (ISKNV). Samples from four Indonesian provinces (West Java, Central Java, East Java and Yogyakarta) were tested molecularly for these viruses. The results revealed widespread viral pathogen involvement, with ISKNV prevalence ranging from 20% to 100% and TiLV prevalence consistently at 100%. These findings strongly suggest that the outbreaks were primarily caused by TiLV and ISKNV infections, potentially with other co-infecting pathogens. Bacterial pathogens, notably Aeromonas spp., Mycobacterium spp., and Pseudomonas spp., may have exacerbated the disease. This research highlights the emergence of viral diseases as a significant threat to giant gourami populations in Java, Indonesia.

Evolution and emergence of mosquito-borne viruses of medical importance: towards a routine metagenomic surveillance approach

Abstract During the last two decades, the world has witnessed the emergence and re-emergence of arthropod-borne viruses, better known as arboviruses. The close contact between sylvatic, rural and peri-urban vector species and humans has been mainly determined by the environment-modifying human activity. The resulting interactions have led to multiple dead-end host infections and have allowed sylvatic arboviruses to eventually adapt to new vectors and hosts, contributing to the establishment of urban transmission cycles of some viruses with enormous epidemiologic impact. The metagenomic next-generation sequencing (NGS) approach has allowed obtaining unbiased sequence information of millions of DNA and RNA molecules from clinical and environmental samples. Robust bioinformatics tools have enabled the assembly of individual sequence reads into contigs and scaffolds partially or completely representing the genomes of the microorganisms and viruses being present in biological samples of clinical relevance. In this review, we describe the different ecological scenarios for the emergence of viral diseases, the virus adaptation process required for the establishment of a new transmission cycle and the usefulness of NGS and computational methods for the discovery and routine genomic surveillance of mosquito-borne viruses in their ecosystems.

Current understanding of multi-country human monkeypox outbreak: a narrative review

<p>Outbreaks of infectious diseases have occurred throughout history. They appear to be increasing in frequency, particularly because of the increasing emergence of viral diseases from animals. Monkeypox is a zoonotic orthopoxviral, similar to smallpox, although with lower mortality. Cases of human monkeypox are rarely seen outside of west and central Africa. The current outbreak is the first of its kind where the transmission is reported in Europe without known links to Africa. India reported the first case of monkeypox from the Southeast Asia region in the state of Kerala, on July 14, 2022. India till now (August 2022) has reported nine monkeypox cases and one death owing to the viral zoonosis infection. It causes flu-like symptoms such as fever, chills, and rash, usually mild, and most patients recover without therapy. As with most viral illnesses, the treatment's mainstay of clinical management for a typical monkeypox infection is supportive symptom management. Acting quickly and proactively, focusing on building surveillance, promptly identifying monkeypox infection, and implementing preventive measures will be vital for containing this pandemic.</p>

Metagenomics show high spatiotemporal virus diversity and ecological compartmentalisation: Virus infections of melon, Cucumis melo, crops, and adjacent wild communities.

The emergence of viral diseases results from novel transmission dynamics between wild and crop plant communities. The bias of studies towards pathogenic viruses of crops has distracted from knowledge of non-antagonistic symbioses in wild plants. Here, we implemented a high-throughput approach to compare the viromes of melon (Cucumis melo) and wild plants of crop (Crop) and adjacent boundaries (Edge). Each of the 41-plant species examined was infected by at least one virus. The interactions of 104 virus operational taxonomic units (OTUs) with these hosts occurred largely within ecological compartments of either Crop or Edge, with Edge having traits of a reservoir community. Local scale patterns of infection were characterised by the positive correlation between plant and virus richness at each site, the tendency for increased specialist host use through seasons, and specialist host use by OTUs observed only in Crop, characterised local-scale patterns of infection. In this study of systematically sampled viromes of a crop and adjacent wild communities, most hosts showed no disease symptoms, suggesting non-antagonistic symbioses are common. The coexistence of viruses within species-rich ecological compartments of agro-systems might promote the evolution of a diversity of virus strategies for survival and transmission. These communities, including those suspected as reservoirs, are subject to sporadic changes in assemblages, and so too are the conditions that favour the emergence of disease.

Exogenous Application of dsRNA for the Control of Viruses in Cucurbits.

The recurrent emergence of viral diseases in intensive horticultural crops requires alternative control strategies. The topical application of double-stranded RNA (dsRNA) molecules homologous to pathogens has been proposed as a tool for virus control in plants. These dsRNAs induce the silencing mechanism, the RNA interference (RNAi), that degrades homologous dsRNAs. Cucumber green mottle mosaic virus (CGMMV) represents a serious threat to cucurbit crops. Since genetic resistance to the virus is not yet available in commercial varieties, we aimed to control this virus by RNAi. For this purpose, we obtained constructions both for expressing dsRNA in bacteria to treat cucumber plants by topical application and for agroinoculation in experiments done in the growth chamber. Besides, greenhouse tests were performed in spring and in summer when plants were challenged with the virus, and differences in several parameters were investigated, including the severity of symptoms, dry weight, total height, virus accumulation, and virus-derived small interfering RNAs (vsiRNAs). Spraying of plants with dsRNA reduced significatively CGMMV symptoms in the plants in growth chamber tests. Agroinfiltration experiments done under identical conditions were also effective in limiting the progress of CGMMV disease. In the greenhouse assay performed in spring, symptoms were significantly reduced in dsRNA-sprayed plants, and the development of the plants improved with respect to non-treated plants. Virus titers and vsiRNAs were clearly reduced in dsRNA-treated plants. The effect of protection of the dsRNA was less evident in the greenhouse assay carried out in the summer. Besides, we investigated the mobility of long (ds)RNA derived from spraying or agroinfiltrated dsRNA and found that it could be detected in local, close distal, and far distal points from the site of application. VsiRNAs were also detected in local and distal points and the differences in accumulation were compared. In parallel, we investigated the capacity of dsRNAs derived from genes of tomato leaf curl New Delhi virus (ToLCNDV), another economically important virus in cucurbits, to limit the disease in zucchini, both by agroinfiltration or by direct spraying, but found no protective effect. In view of the results, the topical application of dsRNAs is postulated as a promising strategy for CGMMV control in the cucumber.

66 Utilizing Third Generation Sequencing to Identify Pathogen Outbreaks Rapidly and Accurately

Abstract The temporal trend of novel pathogen identification has increased over the last decade, with viruses being the main contributor to the increase. Currently, the NAHLN provides veterinary surveillance and testing procedures for high consequence animal diseases, where in many cases, the detection of these diseases is based on real-time PCR assay (ASF, CSF, FMD, and IAV-S) or pathogen specific antibodies using ELISA methods (PRV). However, genetic shift and drift in virus genomes can lead to failure in detection of novel or emerging pathogens. Therefore, with the current trend of increased viral disease emergence, novel methods are needed to accurately and rapidly identify and characterize viral pathogens independent of knowing genomic information. In this study, we developed a novel diagnostic approach to identifying swine pathogens, using surrogate viruses. We implemented the use of Oxford Nanopore MinION Technology to detect long read sequences in real time. Surrogate viruses were used in place of common pathogens such as African Swine Fever, Classical Swine Fever, Pseudorabies, Foot and Mouth Disease, and Influenza A to investigate rapid detection limits. To increase the complexity of a sample, both DNA and RNA viruses were spiked into tissues were analyzed using Oxford Nanopore MinION Technology. This method can detect both DNA and RNA viruses simultaneously without prior knowledge. Additionally, as sequences can be detected real-time, we were able to confidently detect viral pathogens within a sample within 30 min. Additionally, we have established a library preparation and sequencing protocol as well as an informatic pipeline that could allow clinics and diagnostics centers to identify pathogens in less than 10 hours.

Immunological insights into the resistance of Nile tilapia strains to an infection with tilapia lake virus

The emergence of viral diseases affecting fish and causing very high mortality can lead to the disruption of aquaculture production. Recently, this occurred in Nile tilapia aquaculture where a disease caused by a systemic infection with a novel virus named tilapia lake virus (TiLV) caused havoc in cultured populations. With mortality surpassing 90% in young tilapia, the disease caused by TiLV has become a serious challenge for global tilapia aquaculture. In order to partly mitigate the losses, we explored the natural resistance to TiLV-induced disease in three genetic strains of tilapia which were kept at the University of Göttingen, Germany. We used two strains originating from Nilotic regions (Lake Mansala (MAN) and Lake Turkana (ELM)) and one from an unknown location (DRE). We were able to show that the virus is capable of overcoming the natural resistance of tilapia when injected, providing inaccurate mortality results that might complicate finding the resistant strains. Using the cohabitation infection model, we found an ELM strain that did not develop any clinical signs of the infection, which resulted in nearly 100% survival rate. The other two strains (DRE and MAN) showed severe clinical signs and much lower survival rates of 29.3% in the DRE strain and 6.7% in the MAN strain. The disease resistance of tilapia from the ELM strain was correlated with lower viral loads both at the mucosa and internal tissues. Our results suggest that the lower viral load could be caused by a higher magnitude of a mx1-based antiviral response in the initial phase of infection. The lower pro-inflammatory responses also found in the resistant strain might additionally contribute to its protection from developing pathological changes related to the disease. In conclusion, our results suggest the possibility of using TiLV-resistant strains as an ad hoc, cost-effective solution to the TiLV challenge. However, as the fish from the disease-resistant strain still retained significant virus loads in liver and brain and thus could become persistent virus carriers, they should be used within an integrative approach also combining biosecurity, diagnostics and vaccination measures.\\

Significance of a neglected tropical disease: lessons from a paradigmatic case of 'success in translation'.

In a previous publication, I stressed the fundamental importance of research for improving health using as an example the control of Chagas disease in the Americas.(1) For that purpose, I analysed the major scientific breakthroughs and public health events from the 1909 discovery of Chagas disease and its causative pathogen, Trypanosoma cruzi, by Carlos Chagas,(2) through the successful control of its transmission by insect vectors in large regions of the Southern Cone countries in the 90s.(3) In the twenty years since that publication, Brazil and Latin American countries had to cope with a number of serious public health threats, old and new: (i) recrudescence of well-known diseases, such as dengue and yellow fever; (ii) emergence of viral diseases that had been restricted to other continents (Zika, Chikungunya); (iii) new epidemics (H1N1) or (iv) pandemics (COVID-19). Are there still some lessons from that success story against a neglected disease of the 90s that would be relevant today in the context of these recent challenges?

Optimization: Molecular Communication Networks for Viral Disease Analysis Using Deep Leaning Autoencoder.

Developing new treatments for emerging infectious diseases in infectious and noninfectious diseases has attracted a particular attention. The emergence of viral diseases is expected to accelerate; these data indicate the need for a proactive approach to develop widely active family specific and cross family therapies for future disease outbreaks. Viral disease such as pneumonia, severe acute respiratory syndrome type 2, HIV infection, and Hepatitis-C virus can cause directly and indirectly cardiovascular disease (CVD). Emphasis should be placed not only on the development of broad-spectrum molecules and antibodies but also on host factor therapy, including the reutilization of previously approved or developing drugs. Another new class of therapeutics with great antiviral therapeutic potential is molecular communication networks using deep learning autoencoder (DL-AEs). The use of DL-AEs for diagnosis and prognosis prediction of infectious and noninfectious diseases has attracted a particular attention. MCN is map to molecular signaling and communication that are found inside and outside the human body where the goal is to develop a new black box mechanism that can serve the future robust healthcare industry (HCI). MCN has the ability to characterize the signaling process between cells and infectious disease locations at various levels of the human body called point-to-point MCN through DL-AE and provide targeted drug delivery (TDD) environment. Through MCN, and DL-AE healthcare provider can remotely measure biological signals and control certain processes in the required organism for the maintenance of the patient's health state. We use biomicrodevices to promote the real-time monitoring of human health and storage of the gathered data in the cloud. In this paper, we use the DL-based AE approach to design and implement a new drug source and target for the MCN under white Gaussian noise. Simulation results show that transceiver executions for a given medium model that reduces the bit error rate which can be learned. Then, next development of molecular diagnosis such as heart sounds is classified. Furthermore, biohealth interface for the inside and outside human body mechanism is presented, comparative perspective with up-to-date current situation about MCN.

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species.

Understanding biological mechanisms that regulate emergence of viral diseases, in particular those events engaging cross-species pathogens spillover, is becoming increasingly important in virology. Species barrier jumping has been extensively studied in animal viruses, and the critical role of a suitable intermediate host in animal viruses-generated human pandemics is highly topical. However, studies on host jumping involving plant viruses have been focused on shifting intra-species, leaving aside the putative role of “bridge hosts” in facilitating interspecies crossing. Here, we take advantage of several VPg mutants, derived from a chimeric construct of the potyvirus Plum pox virus (PPV), analyzing its differential behaviour in three herbaceous species. Our results showed that two VPg mutations in a Nicotiana clevelandii-adapted virus, emerged during adaptation to the bridge-host Arabidopsis thaliana, drastically prompted partial adaptation to Chenopodium foetidum. Although both changes are expected to facilitate productive interactions with eIF(iso)4E, polymorphims detected in PPV VPg and the three eIF(iso)4E studied, extrapolated to a recent VPg:eIF4E structural model, suggested that two adaptation ways can be operating. Remarkably, we found that VPg mutations driving host-range expansion in two non-related species, not only are not associated with cost trade-off constraints in the original host, but also improve fitness on it.

Part 1: COVID-19 and knowledge for midwifery practice—impact and care of pregnant women

The emergence of viral diseases, such as COVID-19, represents a global public health threat, particularly the high-impact animal viruses that have switched hosts and are able to be transmitted within human populations. Pandemics threaten the general population; however, there are special groups, such as pregnant women and their babies, which may be at a higher risk of, or more severely affected by infection. Pregnancy is considered a unique immunological condition; therefore, current challenges include decisions on preventing and treating infections during pregnancy and the possible implications for the fetus and newborn infant. This integrative review, the first of a two-part series, analyses selected literature on COVID-19 within maternal and newborn care, drawing on key themes relating to the impact on the pregnant woman. The themes discussed are: the nature of the immune system in pregnant and newly birthed mothers, maternal risk, mode and timing of birth, care during pregnancy and childbirth, and the transition to parenthood including the implications for practice for maternal mental wellbeing.

Developing therapeutic approaches for twenty-first-century emerging infectious viral diseases.

The twenty-first century has already recorded more than ten major epidemic or pandemic virus emergence events, including the ongoing and devastating coronavirus disease 2019 (COVID-19) pandemic. As viral disease emergence is expected to accelerate, these data dictate a need for proactive approaches to develop broadly active family-specific and cross-family therapeutics for use in future disease outbreaks. Emphasis should focus not only on the development of broad-spectrum small-molecule and antibody direct-acting antivirals, but also on host-factor therapeutics, including repurposing previously approved or in-pipeline drugs. Another new class of therapeutics with great antiviral therapeutic potential is RNA-based therapeutics. Rather than only focusing on known risks, dedicated efforts must be made toward pre-emptive research focused on outbreak-prone virus families, ultimately offering a strategy to shorten the gap between outbreak and response. Emphasis should also focus on orally available drugs for outpatient use, if possible, and on identifying combination therapies that combat viral and immune-mediated pathologies, extend the effectiveness of therapeutic windows and reduce drug resistance. While such an undertaking will require new vision, dedicated funding and private, federal and academic partnerships, this approach offers hope that global populations need never experience future pandemics such as COVID-19.

Selection of adjuvants in the design of an inactivated vaccine against respiratory infections of cattle

Infectious diseases of young farm animals are a serious problem for the livestock industry. Modern industrial technology of keeping animals, contributing to the emergence of viral diseases, often leads to a decrease in the immunological reactivity of their body, due to the underdevelopment of the immune system of young animals (primary immunodeficiency), food toxicosis, insufficient and unbalanced in various components feeding, as well as the impact of stress factors inherent in industrial technology – rearing animals, transportation, microclimate changing, forming large groups of animals, small feeding area, intensive exploitation. These factors negatively affect the immune system and metabolic processes, which leads to a significant decrease in their resistance to infectious diseases. In turn, the causative agents of viral respiratory diseases inhibit the cellular and humoral components of the immune system. Epizootological monitoring shows that viruses of infectious rhinotracheitis, viral diarrhea, parainfluenza-3 and respiratory syncytial infection in cattle are the main cause of pathological processes in respiratory diseases of young animals. Animal specific prophylactic methods are effective in preventing infectious diseases. Immunization of a pregnant herd promotes the birth of young animals with a full-fledged immune system. In mixed infections, it is difficult to determine the leading role of a particular infectious agent, therefore the most effective method for preventing such diseases is associated vaccines. In the process of designing a vaccine, the selection of antigens and an adjuvant is of great importance, contributing to the formation of a specific and long-term immunity. We studied the comparative efficacy of adjuvants and the antigenic activity of the selected viral strains of infectious rhinotracheitis, viral diarrhea, parainfluenza-3 and respiratory syncytial infection of cattle. The dynamics of biosynthesis of antiviral antibodies after immunization in cows were determined.

Begomoviruses affecting pulse and vegetable crops are unevenly distributed in distinct agroecological zones of the eastern India

AbstractIntensive cultivation, climate change and host–pathogen dynamics/interaction had led to emergence of viral diseases and vector population, thereby causing significant economic losses. In the present study, extensive surveys were conducted (during 2016–2017) followed with field experiments (2017 and 2019 kharif and 2018 rabi seasons) in the state of Chhattisgarh, India, comprising of three diverse agroecological zones, viz., northern hill zone, central plain zone and southern Bastar plateau zone, for understanding the distribution pattern of viruses and vector populations in prominent pulses and vegetable crops. Field experiments revealed that more than 90% incidence of yellow mosaic disease (YMD) was recorded in mungbean and urdbean, followed by YMD of ridge gourd and bitter gourd, leaf curl disease of tomato (ToLCD) and chilli and yellow vein mosaic disease of bhendi; however, only five per cent YMD was recorded in cow pea. Molecular and sequence analysis of viral genomic components revealed ToLCD in central plain zone of Chhattisgarh was caused by a consortium of begomoviruses, including Tomato leaf curl New Delhi virus (ToLCNDV), Tomato leaf curl Karnataka virus (ToLCKV), and Tomato leaf curl Kerala virus associated with satellite molecules. YMD of mungbean, cow pea and soybean was caused by Mungbean yellow mosaic India virus (MYMIV), while YMD of ridge gourd was caused by ToLCNDV. PCR analysis, using species‐specific primers, of samples representing the three different regions revealed that YMD of mungbean, urdbean and cowpea was positive only for MYMIV and was unevenly distributed, while YMD of ridge gourd, bitter gourd, pumpkin and sponge gourd was positive only for ToLCNDV; however, ToLCD was positive for either ToLCNDV or ToLCKV or both, and some samples were also found associated with betasatellite molecules. Amaranthus and rice bean were identified as non‐host species for the begomoviruses infecting tomato and MYMIV, as observed by surveys in the hot‐spot‐like areas, controlled whitefly transmission as well as agroinoculation. Compilation and analysis of available information on begomoviruses affecting these crops in India suggest that they are unevenly distributed in India. This is the first report of the begomovirus occurrence in pulses and vegetable crops in this agroecologically diverse region.

ПРОЯВ ЗАГАЛЬНОПЛАНЕТАРНОЇ КРИЗИ НА ПОЧАТКУ ТРЕТЬОГО ТИСЯЧОЛІТТЯ

The article reveals the essence and preconditions of the global crisis in nature and society at the beginning of the third millennium. The purpose of human life as a manifestation of its society is defined in accordance with the requirements of the objectively existing laws of human evolution on the way to extracurricular society. In particular, attention is paid to such cataclysms of the planet that interfere with human life in the present and future, such as earthquakes, devastating typhoons, temperature fluctuations, downpours, tsunamis, or, conversely, droughts that lead to vegetation, crop failures, forest fires and more , to eliminate which humanity has limited opportunities. A significant impact on the state of natural conditions of our planet is caused by changes in solar activity. Changes in the survival of mankind, such as the emergence of viral diseases, including plague, Ebola, coronavirus and others, which endanger human life and lead to changes in production and living conditions, which in turn forces people to join forces in the struggle for survival. Possible ways to eliminate or mitigate the devastating effects of the planetary crisis in the context of human existence through socio-economic restructuring within the modern era, the trends of which are currently manifested in the socio-economic confrontation of such countries, on the one hand as the United States, on the other – Russia and China, which are heading to the social and economic space. The main provisions of the methodology for quantifying social changes on the path of socio-economic restructuring of mankind in the future by indicators: the level of labor potential, human and physical capital, wages, as representatives of existing industrial relations in the world. In particular, methods have been invented to analyze and calculate the level of wages as an economic category of the transition period to the direct social order in the future of mankind on such elements as the minimum, additional and stimulating wages. The scientific approaches to determining the degree of social maturity of society in the present and future, which distort the progress of mankind to its ultimate goal – the restructuring of extracurricular, direct community structure, its existence on the planet.

ASPECTS OF IMMUNIZATION OF BIRDS BY CULTURAL VACCINES AGAINST DISEASES FOWL POX

A serious problem for the development of industrial avian diseases is the emergence of viral diseases, in particular fowl pox virus. Improvement of dysfunctional farms for fowl pox, elimination of foci of pathogens are the most difficult problems facing modern veterinary science and practice. Outbreaks in poultry farms, leading to the need for veterinary and sanitary measures, are a way to protect the poultry from infection on the farm, which is very expensive. In recent years, attempts have been made to improve the means for specific prophylaxis of avian fowl pox diseases. This article describes the widespread use of the cultural vaccine in poultry farming, notes the promise of a cultured cell system, quail embryos, characteristic of economy, the absence of extraneous contaminants, the stability of biological properties, which facilitates the work with them, and is of no small importance in the production of vaccines.
 The article describes the analysis of the possible cultivation of the strain "Baku".
 It is also shown that embryos of Japanese origin are more convenient and advantageous tissues for the cultural production of primary cell systems, as well as for biological mass, vaccine production, simplicity, economy, lack of extraneous contaminants and biological properties. It is shown that the culture of the cells of Japanese quail embryos is a promising system for creating highly immunogenic agents that provide special protection against diseases lacking extraneous contaminants and stable biological properties.

Uncovering and defragmenting the role of the Toll pathway in the innate immune responses of cultured crustaceans against viral pathogens

AbstractShrimp farming is considered the fastest growing aquaculture industry in the last decades, with an average growth in production of ~15% per year. Unfortunately, this industry has been affected by the emergence of viral diseases, and currently, there are no commercially available therapeutics against these pathogens. Therefore, a deeper understanding of the immune system components induced by viruses is imperative for the development of antiviral therapies in these species. It is well known that the Toll‐like receptor (TLR) signalling is involved in activating the innate immune responses in crustaceans. Although numerous research papers deal with the response of the individual components of this pathway involved in the antiviral response on different crustacean species, the information is highly fragmented. This review provides a detailed picture of the general structure and role of Spätzle, Toll receptors, MyD88, Tollip, Tube, Pelle, Pellino, Dorsal, Cactus and DIF, the elements constituting the Toll‐like receptor participating in the innate immune response against viral diseases in crustaceans. It summarizes the current knowledge progress about the influence of the Toll pathway components on the expression of antimicrobial peptides involved in the antiviral response of crustaceans. In addition, this review compares the structural and functional roles of the elements identified in crustaceans, with those of the popular model organism Drosophila. This systematic review may contribute to identify the existing gaps in the knowledge of the Toll pathway in crustaceans and help to carry out further studies that may be useful for the management of viral diseases.