Concentrations Of EMS Research Articles

COMPLEXATION OF SILVER (I) C 2-ETHYLIMIDAZOLE

The acid-base properties of 2-ethylimidazole were studied by pH-metric titration (EM). Using the literature data and our own results, a number of changes in the acid-base properties (pКa) of imidazoles have been established: ethylimidazole (7,93)> imidazole (7.05)>2-phenylimidazole (6,48)>2-aminobenzimidazole (6,11)> benzimidazole (5,53)>2-phenylbenzimidazole (5,23)>2-mercaptoimidazole (2,97)>1-methyl-2-mercaptoimidazole (2,11). Method The process of complexation of silver(I) with 2-ethylimidazole in the temperature range of 273-318K was investigated using potentiometric titration using a silver electrode. It has been established that the increase in temperature does not affect the stoichiometric composition of complex particles formed in the Ag-EM-H2O system. The results obtained show that as the temperature increases, the stability constants decrease. On the basis of diagrams, the distribution of complex particles depending on the ligand concentration is shown that in System Ag-EM-H2O is mainly dominated by the bis-substituted complex, the yield of which at EM concentrations above 1·10-2 mol/l is 100%. The thermodynamic functions of the formation of 2-ethylimidazole silver complexes have been calculated. It has been established that complexation reactions are exothermic. Spontaneous occurrence of complexation reactions in the system Ag-EM-H2O contributes to a negative change in enthalpy.

Inducing mutation and ascertaining lethal dosage of in vitro cultures of banana cv. Ney Poovan to ethyl methane sulfonate

In vitro mutation breeding in vegetatively propagated crops like banana offers a benefit in screening for beneficial variants in plant cells or cultured tissues. An attempt was made to induce mutants and determine the lethal dose, as it is the prerequisite to optimize the concentration and duration of the mutagen used to recover a larger population in mutation research. Shoot tip cultures were treated for 2 and 4 h at six different EMS concentrations ranging from 80 mM to 160 mM, whereas proliferating multiple shoots were exposed for 30 and 60 min at six different EMS concentrations ranging from 8 mM to 40 mM. Survival percentage, shoot length, and number of shoots reduced linearly and significantly as concentration and duration increased in both shoot tips and proliferating multiple buds. The probit curve-based analysis of mortality of treated explants revealed that the LD50 was 155.83 mM for 2 h and 113.72 mM for 4 h, respectively for shoot tip cultures, whereas for proliferating multiple buds, the LD50 value was adjusted to 39.11 mM for 30 min and 30.41 mM for 60 min. 160 mM EMS for 4 h resulted in a shorter shoot, a longer rooting duration, a lesser number of roots, and decreased root development. In proliferating multiple shoots, the smallest shoot, longest rooting duration, least number of roots, and shortest root were observed in 40 mM EMS for 60 min. Similar reductions in growth parameters were observed in proliferating multiple shoots at higher exposure to EMS for a longer duration.

Development and phenotypic screening of gamma rays and ethyl methanesulphonate mutant populations in tartary buckwheat (Fagopyrum tartaricum (L.) Gaertn)

Tartary buckwheat (F. tartaricum) is an annual self-pollinated pseudo-cereal crop of the Polygonaceae family which is grown worldwide for its nutritive and medicinal value. In order to increase genetic variability and develop genetic resources for crop improvement, gamma rays and EMS were used to develop mutagenized populations in F. tartaricum. In the present study, the biological effect of different doses and concentration of gamma rays and EMS on F. tartaricum to assess the sensitivity of mutagens to create genetic variability was determined. The results revealed that 219.15 Gy of gamma rays and 0.49 % of EMS were optimum for conducting mutagenesis in buckwheat. Both mutagens showed significant effects on the growth parameters in M1 generation. The mutagenic treatments were efficient in creating numerous types of chlorophyll mutations in M2 generation. The frequency of chlorophyll mutation was 1.67 % and 2.70 % for gamma rays and EMS, respectively. These findings shows that EMS is more efficient and effective at inducing variation than gamma rays. A diverse range of morphological mutants affecting every growth stage were screened with the highest frequency in 150 Gy gamma rays and 0.3 % of EMS treatment. . Six morphological mutant types influencing plant height, leaf morphology, growth habit, days to flowering, maturity period, seed yield and seed morphology emerged from induced phenotypic changes in individual plants of the M2 population were identified and selected. This study demonstrated the effectiveness of gamma rays and EMS in increasing genetic diversity and the successful execution of induced mutagenesis in the buckwheat. These mutants may be a valuable source of many beneficial genes in hybridization programs. It is anticipated that both these populations will be a valuable resource for functional genomics research in buckwheat.

In-vitro Screening of EMS and Sodium Azide Induced Mutant Population at Seedling Stage for Drought Tolerance in Wheat (Triticum aestivum L.)

Drought caused by climate change result in water shortages, decreased global wheat production and unevenly distributed heavy rains. About 50% of the global wheat production is affected by water deficit conditions. The effects of different concentrations of EMS and SA on HD-3226 and HI-1620 wheat genotype for drought tolerance capability were investigated at seedling stage under water and osmotic stress conditions. In the present study, 15% concentration of polyethylene glycol (PEG-6000, to mimic drought stress) was used for in-vitro screening of EMS and SA induced mutant lines of both wheat genotypes. Current study revealed that SA decreased germination of seeds, seedling length, seed vigour index and survival rate percentage while it increased morphological mutation frequency percentage as comparison to EMS treatment in both wheat genotypes under water and PEG stress. Moreover, osmotic stress significantly reduced seed germination, shoot length, root length, seed vigour index, survival rate percentage compared to control-wild type.

Optimization of Chemical Mutagen Treatment Techniques and Determination of Absorption Dose in Jasminum auriculatum Ecotype “Muthu Mullai” for Inducing Variation

Background: Chemical mutagenesis is one of the effective tools for the induction of variation in plants. The biological effect of the chemical mutagen on plant tissues is determined by the absorption dose which is calculated by the product of the concentration of the mutagen and the treatment duration. Standardizing the treatment conditions for the best possible mutagenesis is the first prerequisite in chemical mutagenesis. Methods: Five concentrations of EMS (5 mM, 10 mM, 15 mM, 20 mM and 25 mM), three treatment durations (1 hr, 2 hr and 3 hr) and two pre-treatment conditions (soaking in water and non-soaking in water) were taken into consideration in the current study to examine the significance of each factor in regulating the mutation and to determine the unknown value of “Absorption dose” from the known survival rate. Result: The present study revealed that pretreatment with water affected the absorption of EMS into plant tissues and an increase in the EMS concentration and treatment duration decreased the survival rate of the cuttings. The study led to the inference that pretreatment of cuttings of Jasminum auriculatum ecotype Muthu Mullai with water for 2 hours and treatment with EMS at different concentrations of EMS ranging from 5 mM to 30 mM for a duration of 2 hours is suggested for higher mutation frequency.

Induced Mutagenesis and Variability Study in the M2 Generation of Garden Pea (Pisum sativum L.)

Background: Mutation induction in garden pea (Pisum sativum L.) aimed to study the induced genetic variability and identify desirable mutants in the M2 generation with two varieties of garden pea, Kashi Nandini and Kashi Uday. Methods: The two varieties of garden peas, Kashi Nandini and Kashi Uday, were subjected to mutagenic treatments with the EMS at 0.05, 0.10, 0.15, 0.20, 0.30 and 0.40%. The M1 generation was raised in RBD with two replications during Rabi 2019-20. Plants from three concentrations, 0.10, 0.15 and 0.20% EMS, were evaluated in M1 plant progenies (M2) during Rabi 2020-21. The M2 was raised during Rabi 2020-21 in M1 plant progenies using M1 plants from three concentrations -0.10, 0.15 and 0.20% EMS of both varieties. Result: The two varieties that were exposed to EMS responded differently depending on the concentrations. The GCV and PCV were high for seedling height, fertile branches, pods/plant and seeds/pod in both parents at 0.15 and 0.20% EMS. The highest heritability and genetic advance were apparent for seedling height and seeds/pod at 0.10 and 0.20% EMS, while the estimates were moderate for seeds/pod with 0.20% EMS, followed by seeds/pod at 0.10% EMS and seedling height at 0.20% EMS. Desirable plants were visible for various quantitative traits in the M2 generation. These mutants showed significant differences from the controls of both varieties in the treated populations at higher concentrations of EMS. Desirable micro mutants in M2 resulted at 0.15 and 0.20% EMS, which need further evaluation in the M3 for confirmation.

Phenotype performance of M1 generation Bima Shallot (Allium cepa var ascalonicum) result of Ethyl Methane Sulfonate induced

The diversity of crops can be produced via mutagen induction. Its result can be utilized as a source of genetic material in the plant breeding activities to produce a high-quality variety. The purpose of this study was to investigate the phenotype performance of Bima varieties of Shallot (Allium cepa var ascalonicum) result of Ethyl Methane Sulfonate induced. This study used a randomized complete block design, with the treatment of five levels of EMS concentrations. Data obtained were analyzed of variance and if EMS treatment was significant difference that will be proceeded to Duncan Multiple ranged Test. The present study found that EMS treatment differed significantly for all parameters (p< 0.05). The first generation of Bima shallot exhibited growth inhibition and decrease of yield with increasing of EMS concentration than control. The reduction of these parameters may be caused by genetic changing and physiologically damage as consequences of EMS treatments. The EMS treatment of 100 ppm displayed a higher value for all parameters compared to 200, 300, and 400 ppm of EMS.

Induced chlorophyll and morphological mutations in Bambara Groundnut (Vigna subterranea (L.) Verdc.)

The present study was conducted at K-block, University of Agricultural Sciences, Bengaluru. Theexperimental material for the present investigation consists of two varieties of Bambara groundnut havinggood agronomic base belonging to different accessions viz., SB-42 (light brown, round shape and whitehilum) and S165A (dark brownish, dark spotted surface and oval shape). Five different concentrations ofEMS 0.1, 0.2, 0.3, 0.4 and 0.5 per cent were used to treat the seeds. After the treatment, the seeds were sownin the field along with control (untreated seeds) to raise M1 generation. The M2 generation was raisedduring 2014 summer season by pooling all the seeds of M1 plants on plant to row basis along with controlplants. In both the varieties of Bambara groundnut, the mutagenic effectiveness and efficiency of mutagenictreatments varied not only between the two varieties but also between the treatments. A concentration of0.3 per cent EMS was more effective than the other concentrations of EMS. Hence 0.3 per cent EMS has beenregarded as LD50 for inducing useful mutations.

Resolving the acute gamma irradiation and ethyl methanesulphonate induced lethality for Jasminum sambac L. (Aiton) cv. Ramanathapuram Gundumalli

Crop improvement in Jasminum sambac through induced mutagenesis entails the insight on the sensitiveness of the individual plant materials of a species to different classes and nature of the mutagens. The current assessment was undertaken to analyse the mutagenic effect of acute gamma irradiation and EMS in Jasminum sambac cv. Ramanathapuram Gundumalli. Semi-hardwood cuttings of the plants were exposed to different dosage levels of gamma rays varying from 0 Gy (control) to 40 Gy and decapitated rooted cuttings were treated with EMS from 0 mM (control) to 90 mM. The M1V1 generation was evaluated by treating the cuttings with four different doses of gamma rays (15 Gy, 20 Gy, 25 Gy and 30 Gy) and three concentrations of EMS (45 mM, 60 mM, 75 mM) formulated using respective LD50 doses.&nbsp; The lethality dosage (LD50) value for the cultivar Ramanathapuram Gundumalli was determined to be 21.37 Gy and 64.57 mM for gamma irradiation and EMS, respectively applying the probit curve analysis. The maximal reduction in the survival of cuttings of 75% and 71% correspondingly in gamma and EMS treatments was discerned at higher doses in comparison to the lower doses. The plants studied in M1V1 generation for growth and flowering characters exhibited variable responses pertaining to differing doses or concentrations of the specific mutagenic means. Keywords: Jasminum sambac, gamma irradiation, EMS, LD50 and semi-hardwood cuttings

Studies on Mutagenic Effectiveness and Efficiency of Gamma Rays and EMS in Kodo Millet (Paspalum scrobiculatum)

Mutation breeding is the defined approach used for crop production and has played a key role in the creation of several varieties. The present research was carried out to induce mutants in kodo millet variety CO 3 using a physical mutagen (gamma rays) and a chemical mutagen (Ethyl Methane Sulphonate) for creating novelty. Seeds were treated with five doses of gamma-rays viz., 100 Gy, 200 Gy, 300 Gy, 400 Gy, and 500 Gy at BARC, Kalpakam and with three concentrations of EMS viz., 24.15 mM, 32.20 mM and 40.25 mM. In the laboratory test, root and shoot lengths of seedlings were decreased with an increase in the dose of gamma rays and EMS. In field study, the germination percentage and survival rate of seedlings were decreased with an increase in the dose of gamma irradiation and EMS. In M2 generation, five types of chlorophyll mutations viz., albino, xantha, striata, chlorina and albomaculata were observed. Xantha and chlorina were observed in all treatments, whereas, striata and albomaculata were observed only in 200 Gy. Based on the biological damages on M2 plants, mutagenic effectiveness and efficiency were estimated. Both mutagenic effectiveness and efficiency reduced with the increase in the dose of irradiation. Regarding height reduction and lethality, the dose of 100 Gy in gamma treatment and 32.20 mM concentration in EMS treatment recorded maximum efficiency. The mutagenic effectiveness was found to be higher at gamma rays irradiated with 100 Gy and in EMS, in 24.15 mM concentration. The 100 Gray dose and 24.15 mM concentration was found to be highly effective for inducing mutation in kodo millet.

Effect of different gamma rays and EMS concentrations on growth and yield of brinjal genotypes in M3 generation

A field experiment was conducted at Horticultural ResearchFarm, Department of Horticulture, Institute of AgriculturalSciences, B.H.U., Varanasi, UP to investigate the effect ofdifferent gamma rays and EMS concentrations on growthand yield of brinjal genotypes in M3 generation. Theexperimental material comprised of three genotypes of brinjali.e. Banarasi Gol, Kashi Taru and Kashi Uttam and thedifferent concentration of mutagens i.e. gamma rays (5, 10,15 and 20kR) and EMS (0.05, 0.10, 0.15 and 0.20% EMS).These mutagens were studied for growth, yield attributesand yield of all three genotypes. The result recorded all thegrowth and yield traits were decreased as the concentrationof both the mutagens increased. The 5kR gamma rays and0.05% EMS was recorded superior with maximum value ofall characters in these treatments. The fruit yield per plantwas increased under 5kR by 39.77, 31.32 and 18.26 % andunder 0.05% EMS 6.62, 4.81 and 2.56% in Banarasi Gol, KashiTaru and Kashi Uttam, respectively over control. The overallexperimental findings showed that the seed treatments withhigher dose of mutagens were negatively responsive for allcharacters including yield of genotypes.

Physio-biochemical Parameters of Ajwain (Trachyspermum ammi L.) Induced by Gamma Rays and EMS

Background: Induced mutagenesis has proved as a crucial implement to create genetic variability for various essential traits. Trachyspermum ammi is one of the most important medicinal seed spices and its oil exhibits various pharmacological properties. Ajwain has been overlooked owing its narrow genetic base and little study has been performed to create genetic variations. Methods: Dry and healthy seeds of ajwain were treated with the different concentration of gamma rays (25,50,75 and 100Gy), EMS (0.1%, 0.2%, 0.3% and 0.4%EMS) and different doses of combination treatments (25+0.1%, 50+0.2%, 75+0.3% and 100Gy+0.4%EMS). Among both the mutagens, gamma rays were found to be more effectual mutagens as compared to EMS. Result: The aim of the present study was to find out the mutagenic consequences of gamma rays, EMS and combination treatments on growth and physio-biochemical parameters of ajwain. Studies show that higher doses of both the mutagens caused significantly negative effect on the growth parameters whereas lower doses have positively influenced the parameters. Result shows lower doses of mutagens (25, 50Gy, 0.1%, 0.2%EMS and 25+0.1%, 50Gy+0.2%EMS) proved to be more effective as it caused less biological damage and therefore would be suitable for inducing the desirable mutations and improving the agronomic traits in ajwain.

Review Paper on Successive Doses of Gamma Irradiation and EMS for Inducing Mutation in Glycine max and Brassica juncea.

Suitable dose of mutagen in mutational breeding play a key role in developing a new mutant. Every plant’s seed have different number of chromosome, time duration of S phase, and morphology of seed like size of seed, seed coat and biochemical composition of seed. On the basis of these characters different type of seeds require different wavelength of gamma rays and different concentration of EMS for inducing mutation in mutational breeding. The present work on successive dose of Gamma irradiation and EMS for Glycine max (Soybean) and Brassica juncea (Indian mustard) helps to select correct doses of mutagen for the suitable crop.

Enhanced production of extracellular lipase by ethyl methane sulfonate from soil fungal strain in submerged and solid-state fermentation condition

Enhancement in the production of enzyme by utilizing different strains of microbe is one of the main prospects in biotechnology. In the present work, ethyl methane sulfonate (EMF) was selected as the chemical mutagen for inducing mutagenesis in fungi. It is a cheap method to induce random mutation as compared to other methods of recombinant technologies. Strain improvement was done by incubating the fungal spore suspension at variable concentrations of EMS i.e. 4% (v/v) and 10% (v/v) for the time period of 60, 90, and 120 min respectively. The set of control was treated with distilled water only. The fungal colonies were found to be maximum in control plate as compared to the EMF exposed plates. The number of fungal colonies was reduced as we raised the exposure time of EMF. Specific activity and the lipase activity of wild strain and hyperproducer were evaluated under the submerged (SmF) and solid-state fermentation (SSF). The wild strain denoted the 3.2 U/ml/min of enzymatic activity under SmF and 15.87 U/g/min of activity under SSF. In contrast, the best enzymatic activity was represented by S2St1 at 10% of EMS after the time period of 60 min i.e. 11.7 U/ml/min under SmF and 99.35 U/g/min under SSF after the time period of 72 hrs. Statistical analysis by using one-way ANOVA determined that the value of F calculated was lower than the F tabulated. So, there was a significant relation between the EMS percentage and time of exposure among the mutated strains. In conclusion, this soil fungal strain can be utilized to produce lipase enzyme for numerous industrial applications.

Effect of different dosage of EMS on germination, survivability and morpho-physiological characteristics of sunflower seedling

Abstract To find out the effect of EMS dosage on sunflower variety BARI Surjamukhi-2, different EMS concentrations were compared at the molecular biology laboratory (MBL), Oilseed Research Centre (ORC), Bangladesh Agricultural Research Institute (BARI), Gazipur. A total of 10 seeds were treated with each treatment and % germination, % survival and early seedling growth was investigated. Decreased % germination was found with the increasing dose of EMS for all the treatment compared to the control, except for T 4 (0.6% EMS) treatment where a 100% seed was found to be germinated. With an exception of treatment T 4, a decreasing trend of % survivability with the increasing dose of EMS was observed. The gradual reduction with the increasing dose of EMS were observed for most of the studied characters such as leaf area, number of internodes, internode length, fresh and dry weight of leaf, dry weight of shoot, and fresh and dry weight of root in comparison to non-treated control seedlings. However, stimulatory effect with lower dosage of EMS was observed for shoot length in T 2 (0.2% EMS), root length in T 3 (0.4% EMS), and stem fresh weight in T 2 over control. Based on a quadratic regression analysis, using % survivability data, LD50 was calculated and optimum EMS dose was found 0.5%. Hence, the optimized EMS dose of 0.5% may be used to develop large scale desirable mutant of sunflower variety BARI Surjamukhi-2.

ETHYL METHANESULPHONATE (EMS) INDUCES DROUGHT TOLERANCE IN MAIZE

THIS STudy was aimed to induce genetic variation toward drought tolerance after treatment with EMS as a mutagen to two maize inbred lines and their single hybrid. The experiment was carried out at Abo-Ghraib Research Station, Baghdad during the spring season of 2019. The experiment included three irrigation intervals 7, 9 and 11, four concentrations of EMS (0, 50, 60, and 70 mM) and three maize genotypes distributed in three replications using randomized complete block design (RCBD) with split-split plot arrangement which included three irrigation intervals 7, 9 and 11 days occupied the main plot, while the sub plots included three maize genotypes, and four EMS concentrations which were distributed in sub-sub plots. Results were revealed significant differences among EMS treatments and irrigation interval treatments. The inbred line 5 in control treatment showed a significant increase in days for both anthesis and silking (70.11 and 71.94) days, respectively. Plants of Nahrain hybrid also showed a super performance in plant height, leaf area, and number of rows ear-1, with a mean of 154.47cm, 746.31cm2 and 17.83 row ear-1, respectively. The irrigation intervals of 9 and 11 days were significantly decreased the mean performance of genotypes, compared to the shortest irrigation interval (7 days) which had highest mean in most traits. None treated plants (0 mM EMS) had a significant effect on the performance of most studied traits, except the anthesis and silking time, which gave the highest values under the concentration 60 mM with 69.11 and 70.48 days, respectively. Hybrid plants interacted significantly with the EMS concentration of 0 mM producing the maximal means of plant height and leaf area (169.56cm and 759.78cm2 respectively). On the other hand, the hybrid was significantly interacted with the shortest irrigation interval in the mean traits of plant's height and leaf area (163.50 cm and 757 cm2, respectively). The triple interaction between hybrids, shortest irrigation interval and EMS concentration of 60 mM had significantly increased leaf area reached 769.67cm2, meanwhile the triple interaction between hybrid plants, shortest irrigation interval, and the EMS concentration of 0 mM recorded the highest value of plant yield (199.67g). It can be concluded from the current study that the genotypes differed in their response to 9 and 11 days irrigation intervals, with a negative effect of the later interval on the vegetative and yield traits of maize compared with shorter irrigation interval.

INDUCED GENETIC VARIABILITY IN SESAME (Sesamum indicum L): A COMPARATIVE STUDY ON THE MUTAGENIC EFFECTS OF RADIATION AND EMS IN SEED GERMINATION, POLLEN VIABILITY AND CHLOROPHYLL MUTANTS

The present study envisaged the effects of two mutagens, gamma rays and EMS on the phenotypes of two sesame varieties viz., TMV7 and SVPR1. A known quantity of dry, uniform, and healthy seeds of TMV7 and SVPR 1 were irradiated using Co60 (Cobalt 60) with different doses (250, 300, 350, 400, 450 Gy) of gamma rays. For chemical mutagenesis, different concentrations of EMS @ 0.20%, 0.40% and 0.60% was used and treated for 8 h. The dose-response curve of the probit analysis showed that the optimal lethal dose for SVPR1was lower than TMV7. The expected LD50 values of gamma radiation for TMV 7 and SVPR1 were 403.91Gy and 343.84Gy, respectively. For EMS, the expected LD50 values are 0.525 % and 0.276% for TMV7 and SVPR1 respectively. Germination and pollen fertility declined linearly with an increase in dose or concentration of the mutagens. Three classes of chlorophyll mutants viz., xantha, chlorine, and viridis in M2 generation reveals a dose dependent relationship between mutagens and frequency of chlorophyll mutants. Mutagenic effectiveness was higher at lower doses whereas mutagenic efficiency was observed higher at extremity doses in both the varieties. The overall considerations on M1 generation effects showed that SVPR1was highly sensitive to gamma rays and TMV7 produced more viable mutationsthan SVPR1. The current studies suggest gamma rays as an efficient mutagen to induce essential mutations in TMV7 for the further crop improvement program.

Being the minority hurts or helps? A moderated mediation model of group membership, cross-cultural acceptance, and school adjustment

This study examined the relations between majority/minority group membership and cross-cultural acceptance, and their linkage to school adjustment. A total of 2,016 students (ethnic minority [EM]: 51%; boys: 50%) at Grades 2, 5, 8, and 11 from 15 schools in Hong Kong participated in the study. These schools were either of low (below 30%) or high EM concentrations (over 70%). EM students at low-EM-concentration schools and Chinese students at high-EM-concentration schools both belonged to the minority groups in their respective schools. Moderated mediation analyses showed that being the numerical minority in school predicted higher school engagement and more positive affect. The associations between numerical group membership and adjustment outcomes were each mediated by the intention to accept outgroup members. In other words, higher cross-cultural acceptance was found among students who were themselves the minority in school, and stronger outgroup acceptance, in turn, predicted better adjustment.

Impact of emamectin benzoate on nucleopolyhedrosis virus infectivity of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

BackgroundAttempts based on increasing the efficacy of Baculovirus and/or reducing the application concentration of synthetic insecticides through integrated lepidopteran management are appreciated role for conserving the environment. Impact of the multiple nucleopolyhedrosis virus (SpliMNPV) with emamectin benzoate (Em) against the cotton leaf worm, Spodoptera littoralis, was examined to identify the effective strategy for applying both agents in the control program successfully.Main bodyThe LC50 and LC90 were drastically decreased from 1.9 × 106 and 1.0 × 1010 PIB/ml in SpliMNPV treatment to reach 8.87 × 101 and 1 × 104 PIB/ml, respectively in the SpliMNPV concentrations + Em LC25 treatment. This interaction was considered as potentiation. Larvicidal activity of Em was highly increased by Em concentrations + SpliMNPV LC25 treatment than the separately Em treatment; however, this interaction was considered as additive. Moreover, the mixture treatment (SpliMNPV LC99 + Em LC50) provided almost full protection of viral pathogenicity up to 48 h at natural exposure periods. Furthermore, the mixture treatment had a negative impact on the insect survival and reproduction of treated individuals.ConclusionResults indicated that the virus infectivity was increased by a mixture treatment of SpliMNPV + Em in particular facing UV sunlight, which causes virus degradation as well as reduced the effective doses of Em. These findings suggest that this simultaneous treatment maybe an effective technique to be applied in S. littoralis control strategy.

Genetic Improvement of Calendula officinal through Mutation Induction using Gamma Irradiation and Chemical Mutagens

Calendulais one of the basic plants of ornamentals. It is used in various purposes as cut flowers and in gardens. They are rich with active compounds such as chlorophyll a and b, phenols, flavonoid, and carotenoids. The present study focused on mutation induction in order to improve the morphology of the plant which can increase the turnout of its market. In order to examine which mutagen will make the most noticeable and permanent improvement of the plant. For the mutagens, seeds were treated with three doses of gamma ray (2500, 5000, 7000Gy) and two chemical mutagens; colchicine and EMS; which were applied at concentrations of 1000, 3000, and 10000 ppm. With the aim to measure the plant improvement, we measured the plant morphology, its active compounds and protein profiling before and after treatments with both γ-radiation and chemical mutagen to see which one has the most effective imprint on the plant. The morphological parameters data showed the highest plant high by the two doses; gamma dose of 7000 Gy and EMS concentration 10000 ppm, while the lowest dose of colchicine (1000 ppm) made the most impact on the plant height. As for the highest number of leaves/plant, it was obtained at 5000 Gy and 10000 ppm of EMS, while 1000 ppm of colchicine had the same effect. But the number of flowers/plant was not affected by the gamma radiation and colchicine but increased significantly by 10000 ppm of EMS. Surprise, the flower diameter was not affected by EMS while decreased at 2500 Gy and 3000 ppm of colchicine. Considering the importance of flower shape, all treatments either gamma radiation or chemical mutagens showed noticeable changes. Whereas, the biochemical parameters measuring the active compounds, both Chl-a, Chl-b and carotenoids contents increased at 5000 Gy, while flavonoid increased at 2500 Gy. Although, all doses of gamma ray eliminated the phenols content. While the lowest used concentration of colchicine (1000 ppm) increased both of Chl-a, Chl-b, flavonoid and carotenoids, even though the 10000 ppm of colchicine increased the phenol. On the other hand, the highest concentration of EMS (10000 ppm) increased Chl-a and Chl-b while carotenoids, flavonoid and phenol increased at 3000 ppm of EMS. The protein-profile analysis showed fluctuation in the gene expression. The highest performance appeared at 7000 Gy of gamma treatment,1000 -3000 ppm of colchicine, while the same result was obtained by EMS at 3000 ppm.