Elite Performance Research Articles

Advocating individual-based profiles of elite athletes to capture the multifactorial nature of elite sports performance

Elite athletes are high-performance outliers within their specific sports. Even though science seeks to understand the nature of expertise and elite performance, much knowledge remains compartmentalized within subdisciplines. Despite this multidimensionality being acknowledged, an interdisciplinary approach to understanding elite athletes is still rare. This paper synthesizes insights across scientific domains in order to describe the population and individual characteristics of elite athletes. We analyzed diagnostic data from approximately 300 German squad athletes across eight different sports (e.g., gymnastics, volleyball, ice hockey, 3 × 3 basketball etc., agefemale = 18.95 ± 4.84 years, agemale = 19.32 ± 4.19 years) with expertise values ranging from 2 (low expertise) to 16 (high expertise). Data covered muscular strength, lower-body dynamics, muscle-power genetics, blood micronutrients, basic cognitive function, mental health, social support, and training conditions. Results of logistic regressions identified basic cognitive function (B = 0.89) and well-balanced blood micronutrients (B = 1.22) as critical factors distinguishing elite athletes. Additionally, multiple linear regressions suggested that lower-body dynamics (ß = 0.72) is related to increasing expertise values. We examined interactions between determinants of elite performance, and found that social support is positively associated with mental health and training conditions, whereas muscular strength correlates with lower-body dynamics. Focusing on top elite athletes in contrast to semi-elite athletes, we found higher within-group similarities in basic cognitive function and blood micronutrients. Findings indicate the need for a systemic, individualized, and comprehensive model using individual-based profiles.

Finding a way in and making it stick: an exploration of chiropractor experiences working in team-oriented elite sport practice settings

Interprofessional healthcare teams have become the benchmark for optimising athlete health and performance in high-stakes sports. Despite a history of utility as provider partners, chiropractors are currently a relatively underutilised...

Return to play and performance after patellar fracture in American professional sports: a case–control cohort analysis

ABSTRACT Objectives This study describes the effects of patellar fracture on return to play (RTP) and functional outcomes among athletes in American professional sports. Methods Professional athletes from the National Football League (NFL), National Basketball Association (NBA), Major League Baseball (MLB), and National Hockey League (NHL) who suffered a patellar fracture between January 1965 and December 2021 were identified through injury reports and public archives. Performance scores, play time, and games played were collected for the season preceding patellar fracture and 2 seasons after RTP, and differences in recorded metrics compared to pre-injury levels and matched controls were analyzed. Results Twenty-nine of 41 (71%) injured athletes returned to play at an average of 217 days. Among all players, play volume decreased in year 1 compared to baseline but recovered to pre-injury levels in year 2. Athletes treated operatively experienced an initial decline in performance (p < 0.01) but recovered to pre-injury performance level in year 2. Nonoperative management resulted in a decline in performance in year 2 of RTP (p = 0.02). Athletes treated operatively performed significantly worse than matched controls in year 1 of RTP (64% vs. 99%; p = 0.04) but recovered to a similar level of performance as controls in year 2 (87% vs. 91%; p = 0.90). Conclusion A 71% rate of RTP was demonstrated among this limited cohort of 29 athletes in American professional sports after isolated patellar fracture. Although details regarding fracture characteristics and operative reports were not available for analysis, operative management was not associated with longer absence from play compared to nonoperative treatment. Despite the limitations of this study, the findings suggesting operative management may improve prospects of maintaining elite performance following RTP warrant further investigation. Level of evidence Case-control cohort analysis; Level of evidence, 3.

Return to Play Outcomes in Elite Athletes after Cervical and Lumbar Motion Preservation Spine Surgery: A Systematic Review.

Systematic Review. The primary purpose of this article was to survey the present literature and report on return to play outcomes in elite athletes after undergoing motion preservation spinal surgery. For elite performance, athletes require adequate mobility and throughout the trunk, torso, and spine to achieve maximal force production. Therefore, elite athletes that have failed conservative treatment may seek to undergo motion preserving surgical options such as total disc arthroplasty and lumbar microdiscectomy. Individual studies have reported on return to play (RTP) outcomes following individual motion preservation surgical procedures, no systematic reviews have formally to reported on RTP outcomes, post-operative performance, and reoperation rates on these procedures in elite athletes. A systematic review was conducted from inception until February 2024 according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. One reviewer queried PubMed for relevant studies that reported on RTP outcomes in elite athletes following motion preservation spinal surgery (MPSS) based on title and abstract (n=1,404). After the original search query, an additional reviewer screened full length articles. A total of 11 studies met the inclusion criteria. Special consideration was given to RTP rates, post-operative performance, and reoperation rates. A total of 612 elite athletes from the National Basketball Association, Major League Baseball, National Football League, National Hockey League, and other professional sporting organizations underwent cervical and lumbar MPSS to treat various spinal pathologies. Various motion sparing techniques were used to treat various pathologies. After undergoing MPSS, RTP rates ranged from 75-100% for lumbar cases and 83.3-100% for cervical cases. Most athletes successfully returned to sport between. Post-operative performance varied with some athletes performing at the same level prior to surgery and some performing at a decreased level. Motion preservation spinal surgery is a feasible option when properly indicated. Future studies are needed to compare return to sport rates, post-operative performance, and re-operation rates between motion preservation spinal surgery to spinal arthrodesis.

Commercial perspectives: Genome editing as a breeding tool for health and well-being in dairy cattle

Genome editing is the latest breeding tool capable of accelerating the rate of genetic improvement for health and well-being traits in food animals. It enables the introduction of beneficial alleles within a single generation, including those of low frequency or absent in the population, while effectively bypassing linkage drag. For the dairy industry, genome editing can be used to make rapid genetic improvements that are precise, efficient, and transgene-free for functional traits that are not practically addressed without disrupting conventional breeding goals for overall economic merit based on genomic selection. Herein, various case studies for dairy cattle breeding are presented that demonstrate applications of genome editing for enhancing heat stress tolerance, reduced disease susceptibility, and/or other qualitative traits absent in some breeds. One case highlights the success of simultaneous editing of multiple loci through recent advancements in embryonic stem cell biology. Multiplexed editing is crucial for addressing the polygenic nature inherent to many economically important traits in livestock. However, maximizing the benefits of genome editing depends on the continued discovery of targets for editing that are commercially important. Commercialization also depends on rapidly evolving regulatory statutes for risk assessment, where some countries already permit the commercialization of cattle with non-GMO genome alterations through existing regulations. New breeding technologies like genome editing are now poised to have significant impact in equipping elite performance cattle to be more resilient to infectious disease and climate change without the loss of production gains obtained from decades of selection.

The importance of paddling to surfing performance: Insights from elite athletes, coaches, and performance support practitioners

Although paddling has been widely studied, there remains a limited understanding of how a surfer's paddling ability directly influences their wave-riding performance. Using an ecological approach, this study examined the importance of paddling to surfing performance from the perspective of elite Australian athletes, coaches, and performance support practitioners. Ten elite Australian surfing coaches, ten elite Australian surfing athletes, and eight performance support practitioners completed semi-structured interviews. This approach underscores the significance of incorporating experiential knowledge from coaches, athletes, and performance support practitioners, particularly in surfing where the nature of high-performance sport requires a multi-disciplinary approach and highlights the potential value of qualitatively exploring expert perspectives. Findings showed that all participants agree that paddling is an integral aspect of surfing performance despite not being directly scored in competition. The ecological dynamics approach provided a framework for understanding the function of paddling as a tool that enables surfers to exploit the affordances of the environment and support positive performance outcomes under highly variable conditions. The findings highlight the complexities of the performer-environment relationship in surfing and draw attention to the potential for improvements in surfing performance that may be elicited by enhancing a surfer's paddling ability. This study contributes to a growing body of sports science literature investigating paddling performance in surfers and underscores the importance of paddling to surfing performance.

The isometric and isokinetic knee extension and flexion muscle strength profile of elite soccer players

BackgroundThis study aimed to further complete normative data sets for the strength profile of the thigh in soccer players by performing isometric and isokinetic measurements in a large sample per player position.MethodsIn total, 364 soccer players were divided into subgroups according to their pitch position. All players performed isometric and isokinetic strength measurements during active competition period using an isokinetic dynamometer (System 3, Biodex®, ENRAF-NONIUS, New York, USA).ResultsIsokinetic strength of m. Quadriceps was significantly (p = 0.023) higher in strikers compared to central midfielders in both the dominant (+ 9%) and the non-dominant (+ 4%) leg. No further differences were found in isometric or isokinetic strength per playing positions.ConclusionsWe have shown that strength profiles do not differ between playing positions on the field. This is valuable information for elite soccer high performance coaches and medical staffs aiming to improve daily training efficiency and rehabilitation of their players.

Neuromechanical proficiency in elite performance decision-making: An event-related potential (ERP) analysis

This research focuses intensively on the neural mechanisms of action selection responses in elite athletes during high-stakes decision-making. It emphasizes the neuromechanical dimensions of these processes, contrasting the responses of expert and novice players within a theoretical framework of neural information processing in high-performance contexts. Utilizing advanced EEG technologies, including event-related potential (ERP) analysis, the study captures a comprehensive view of both behavioral and neurophysiological data. The central aim is to unravel the intricate neural underpinnings that distinguish elite athletes in their decision-making strategies. Key findings highlight: (1) Enhanced accuracy and swifter reaction times in elite athletes during the action selection phase; (2) Significant neurophysiological differences, marked by pronounced N1 peak amplitudes with prolonged latencies, reduced P2 peak amplitudes with stable latencies, decreased P3 peak amplitudes with reduced latencies, and increased average PSW amplitudes. These discoveries significantly advance our understanding of the neural foundations of expert decision-making in high-performance sports. This study not only sheds light on the cognitive and neural dynamics of elite sports performance but also provides a foundation for developing training and performance enhancement techniques in various high-stakes domains.

The association of the ACE gene I/D polymorphism and in-game performance in elite Rugby Union players

Genetic variation has been proposed as a factor in human athletic performance. The angiotensin converting enzyme (ACE) gene has been studied extensively albeit with heterogeneous results. The insertion (I) and deletion (D) alleles have been associated with altered serum and tissue ACE, as well as endurance- and power-based performance respectively. It is suggested the distinction in rugby union performance can be partly attributed to variations in the ACE gene. The aim of this study was to identify associations between the ACE I/D genetic polymorphism and in-game performance in elite (≥ 5 games at top-flight) rugby union athletes. The athlete cohort consisted of 347 Caucasian men, divided into positional groups (backs and forwards) and positional subgroups (front five, back row, halves, centres, and back three). Ethical approval was provided by Manchester Metropolitan University with written informed consent obtained from all participants. Genotyping and genotype analysis was performed using a combination of the X9 High-Throughput Genomics System (Standard BioTools) and the StepOnePlus™ Real-Time PCR System (Applied Biosystems) and their respective corresponding software. IBM SPSS Statistics 28 (SPSS, Chicago, IL) software was used to conduct Pearson's Chi-square (χ2) test of independence comparing genotype and allelic frequencies and the Kruskal-Wallis H and Mann-Whitney U tests to investigate differences between genotypes. Genotypic and allelic frequencies did not differ at the population level, nor at the positional group/subgroup levels. Performance did not differ between genotypes nor alleles at the population and positional group levels. Back row II genotypes beat more defenders than IDs (P = 0.008) but completed fewer tackles than both IDs (P = 0.05) and DDs (P = 0.013). Halfback DDs missed fewer tackles than both IDs (P = 0.003) and IIs (P = 0.01). Centre DD genotypes made more carries than IIs (P = 0.006). Back three IIs scored more tries than both IDs (P = 0.005) and DDs (P = 0.007) and made more clean breaks than IDs (P = 0.004). Most of the existing literature has been concerned with identifying the ACE gene as a marker of elite performance. To our knowledge, this is the first study to elucidate how the inter-personal differences in rugby union performance are influenced by the ACE I/D polymorphism. The findings from this study may provide novel insight into the specialisation of training and rehabilitation modalities for athletes, based on their genetic makeup.

The effects of music on group motivation and team cohesion among novice to intermediate collegiate rowers

Within university level sport, the opportunity to form friendships, connect and create a sense of enjoyment is fundamental to the student experience. The use of music has been shown to have a positive motivational influence within individual and group physical activity settings when engaging in anaerobic activities. Furthermore, team cohesion can be positively influenced by group music self-selection. Within rowing, music with a high number of beats per minute (bpm) has shown to positively affect performance on an indoor rowing machine. Research within this area has primarily provided its focus towards exercisers or teams within an elite performance setting. Therefore, the aims of this study were to explore whether the introduction of music, through group self-selection and playback can have a positive effect upon the group motivation and team cohesion on novice to intermediate level university rowers when encountering a team anaerobic activity. Twenty novice and intermediate university-level rowers from the same participating university rowing club completed the Group Environment Questionnaire (GEQ) and subsequently a team relay of 2000 m, rowing 500 m each in groups of four, with no music stimulus. Eleven music tracks were selected based upon favoured bands or genres of those rowers, and that they contained at least 152 bpm. Tracks were subsequently presented to the participants who were asked to rate the effectiveness of the audio in terms of motivation and cohesion using the Brunel Group Music Rating Inventory (BGMRI). These scores determined the order of the tracks played for the repeat of the team relay. Once completed, the GEQ was readministered. All measures were used in conjunction with institutional ethics approval (33249-A-Feb/2024- 50074-1). A repeated measures MANOVA analysis found a positive difference in one of the four subscales of the GEQ, Group Integration – Social (GI-S; P = 0.03) F1,19 = 5.54, P &lt; 0.05, Hp2 = 0.27). Surprisingly, a supplementary t-test demonstrated a negative difference in the average number of strokes per minute (spm) during the relay t19 = 2.85, P = 0.01. Whilst the data does not replicate previously reported findings when introducing music to anaerobic indoor rowing training, the significant improvement in GI-S scores highlight some real-world implications. With an understanding of the importance of social cohesion in a university environment and the crucial impact sport can create, there is potential to develop a more robust intervention as a social tool first, before becoming a performance enhancement mechanism.

Effect of an endothelial regulatory module on plasma proteomics in exercising horses

Elite performing exercise requires an intricate modulation of the blood pressure to support the working muscles with oxygen. We have previously identified a genomic regulatory module that associates with differences in blood pressures of importance for elite performance in racehorses. This study aimed to determine the effect of the regulatory module on the protein repertoire. We sampled plasma from 12 Coldblooded trotters divided into two endothelial regulatory module haplotype groups, a sub-elite performing haplotype (SPH) and an elite performing haplotype (EPH), each at rest and exercise. The haplotype groups and their interaction were interrogated in two analyses, i) individual paired ratio analysis for identifying differentially abundant proteins of exercise (DAPE) and interaction (DAPI) between haplotype and exercise, and ii) unpaired ratio analysis for identifying differentially abundant protein of haplotype (DAPH). The proteomics analyses revealed a widespread change in plasma protein content during exercise, with a decreased tendency in protein abundance that is mainly related to lung function, tissue fluids, metabolism, calcium ion pathway and cellular energy metabolism. Furthermore, we provide the first investigation of the proteome variation due to the interaction between exercise and related blood pressure haplotypes, which this difference was related to a faster switch to the lipoprotein and lipid metabolism during exercise for EPH. The molecular signatures identified in the present study contribute to an improved understanding of exercise-related blood pressure regulation.

How much and why do political parties talk about terrorism?: evidence from the Basque Parliament (1980–2011)

ABSTRACT This article is focused on the weight that the issue of terrorism had among the discourse of the political parties sitting in the Basque Parliament between 1980 and 2011. In order to explore this question, transcripts of the most generalistic parliamentary debates were hand-coded and transformed into a quantitative variable. Results show that, from 1995 onward, when ETA began to attack politicians and other representatives of civil society, the Basque political elite started to pay more attention to the problem of terrorism in its discourse. Beyond this descriptive result, this study explores which other independent variables have mediated in the time that Basque MPs spent talking about terrorism. Thus, this article contributes to the study of how terrorist tactics influence the performance of the political elite.

The Relation of On-Ice and Off-Ice Performance at Two Different Performance Levels in Youth Ice-Hockey Players.

Ice hockey requires two levels of specific agility, involving different abilities, where the level of agility and their constraints might vary by the performance level. Therefore, this study aimed to compare the relationship level between on-ice and off-ice change of directional speed (COD) of youth hockey players at two performance levels. The study was conducted during the hockey season, including U16 elite players (n = 40) and U16 sub-elite players (n = 23). Both groups performed specific on-ice fitness tests (4-m acceleration, 30-m sprint, and 6 x 54-m tests, an on-ice Illinois agility test with and without a puck) and off-ice tests consisting of non-arm swing countermovement jumps (CMJs), broad jumps, and pull-ups. Pearson correlation showed that the acceleration performance of elite players was related to the CMJ (r = -0.46) and the broad jump (r = -0.31). Sub-elite players showed stronger dependence of the 30-m sprint on the CMJ (r = -0.77) and the broad jump (r = -0.43), the relation of pulls ups (r = -0.62) and the CMJ (r = -0.50) to the 6 x 54-m test, yet no association to acceleration. Elite players differ between off-ice and on-ice performance constraints, where their skating sprint is less related to their vertical and horizontal take-off abilities than in sub-elite players. Sub-elite players' off-ice power determines their sprint and repeated sprint performance. COD performance of elite and sub-elite players is based on different conditioning constraints.

Elite performance in para-cycling: a scoping review

Elite performance in para-cycling: a scoping review

A Comparison of Elite and Sub-elite Match-Play Running Performance of Gaelic Football Players.

Donnelly, S, Collins, K, Burns, C, O'Neill, C, and Mangan, S. A comparison of elite and sub-elite match-play running performance of Gaelic football players. J Strength Cond Res 38(5): 912-923, 2024-This research aimed to examine differences in match-play running performance between elite and sub-elite Gaelic football players. Global Positioning System devices were used to record the match-play running performance of sub-elite and elite players during the 2018 and 2019 seasons, respectively. In total, 783 samples were collected from 31 sub-elite and 30 elite players, from 1 elite and 1 sub-elite team. Comparisons were made on a team and positional level. Statistical significance was accepted at p ≤ 0.05. Significant positional differences were present between all positions with varying effect sizes (ESs) and p-values. Effect size was used to determine the magnitude of statistical difference. On a team level, the elite covered significantly more relative distance (p = 0.021, ES = 0.007) and elicited significantly higher average speed (p = 0.023, ES = 0.007) in quarters 2, 3, and 4 than the sub-elite. The elite covered significantly more relative distance high-intensity running (17-21.9 km·h-1; p = 0.002, ES = 0.012) in quarter 3 and performed significantly more power events per minute (p = 0.006, ES = 0.009) in quarter 4. In quarter 1, the sub-elite performed significantly more power events per minute (p = 0.006, ES = 0.009), relative high-speed running (≥17 km·h-1; p = 0.011, ES = 0.009), and relative distance high-intensity running (17-21.9 km·h-1; p = 0.002, ES = 0.012). Power events were defined as the estimation of the number of events that depend on anaerobic processes. The present study indicates that elite players possess superior conditioning, ability to read match-play, pacing, and anaerobic capacity. Sub-elite teams may use the present study to inform their training to potentially improve conditioning, pacing, and anaerobic capacity.

A CASE STUDY OF THE PHYSIOLOGICAL AND PSYCHOLOGICAL RESPONSES TO COACHING PROFESSIONAL AUSTRALIAN FOOTBALL

INTRODUCTION &amp; AIMS Coaching professional sport is stressful, yet there is little information detailing the physiological and psychological responses of coaches during match-play. The burden of measurement instruments during competition may preclude their use with coaches during this high-pressure part of the coaching cycle. This exploratory case study examined physiological and psychological alterations when coaching professional Australian football to determine the potential health implications and enhance health literacy in coaches. METHODS One head coach of a professional football team was monitored for heart rate (HR), stress-related hormones (C-Reactive Protein, Cortisol, Troponin, Brain Natriuretic Peptide) and psychological stress prior to, during and following seven matches and descriptive data was examined. RESULTS The HR response indicated sustained elevation during match-play, with the intermittent nature of the game causing an undulating profile. Periods of locomotion during breaks in play led to elevations in HR, with maximum HR recorded as 8% above age-predicted maximum. Further, differences in HR were evident in the final five minutes between small (145 ± 7.0 bpm) and large (113 ± 5.1 bpm) score margins. There were no irregularities for stress hormones, while the psychological questionnaire revealed differences in perceptions of accomplishment, success, recovery and stress related to match outcome. CONCLUSIONS This exploratory case study indicated that substantial elevations in HR are evident while coaching professional football, yielding implications for health management. Coaches require appropriate levels of cardiovascular health to cope with the demands of coaching and targeted health intervention programs may be warranted. Further, differences in psychological outcomes from winning or losing may reflect the need to develop recovery and coping strategies that are contextualised to match results. Since match-play observation elicits alterations to physical and psychological markers, confirmatory research with larger cohorts is warranted to examine and enhance well-being and health management strategies in these elite performers.

Landscapes of Performance: Using Local Geography for the Testing of Sport School Pupils in Sweden, 1972–2023

Does nature still matter in sport? In the balancing between natural and scientific training, Swedish upper-secondary ski schools have played an important role. This paper deals with specific landscape features for testing at three Swedish ski schools: Hallstatestet in Sollefteå, Hovfjällsracet in Torsby, and Stoltjonastestet in Järpen. The following questions will be addressed: How do the coaches at each school use local tests to analyze performance? How is the importance of local tests articulated, and what roles do history and nature play in this process? The paper concludes that the use of local landscapes to articulate elite performance connects ideas of measurability and scientization to the lingering tradition of natural training. Local landscapes thereby become a mediator between scientific and experiential knowledge about sport performance and point out how local sport heritage can be used for addressing environmental issues in sport.

Outcomes of clinical studies of mindset and lifestyle in sports performance: a systematic review

Introduction: The athlete's lifestyle and mindset become an integral part of sports performance. The quest to improve the performance of world-class athletes involves understanding the attributes, skills, strategies, support, environment, and lifestyles of elite performers. Objective: It was to develop a systematic review to highlight the main outcomes of clinical studies of mindset and lifestyle in sports performance. Methods: The PRISMA Platform systematic review rules were followed. The research was carried out from September to October 2023 in the Web of Science, Scopus, PubMed, Science Direct, Scielo, and Google Scholar databases. The quality of the studies was based on the GRADE instrument and the risk of bias was analyzed according to the Cochrane instrument. Results and Conclusion: A total of 151 articles were found, and 34 articles were evaluated in full, and 27 were included and developed in the present systematic review study. Considering the Cochrane tool for risk of bias, the overall assessment resulted in 29 studies with a high risk of bias and 44 studies that did not meet GRADE and AMSTAR-2. Most studies showed homogeneity in their results, with X2=61.9%&gt;50%. It was concluded that champion athletes attribute their good mindset as the main predictor of competitive success. Furthermore, championship performance requires a particular way of life that integrates mindset, performance factors, lifestyle, and relationships. High-performance strategies include using sharp mental rehearsal skills and practice retrieval.

Changes of Anaerobic Power and Lactate Concentration following Intense Glycolytic Efforts in Elite and Sub-Elite 400-meter Sprinters.

400-m races are based on anaerobic energy metabolism, they induce significant muscle fatigue, muscle fiber damage, and high blood lactate (LA) concentration. Despite extensive research on sprint training, our understanding of the training process that leads to world-class sprint performance is rather limited. This study aimed to determine differences in LA concentration and anaerobic power using jumping tests after an intense glycolytic effort in a group of elite and sub-elite 400-m runners. One hundred thirty male runners were divided into two groups: elite (n = 66, body mass = 73.4 ± 7.8 kg, body height = 182.1 ± 6.2 cm, age = 20.8 ± 4.0 y) running the 400-m dash below 50 s and sub-elite (n = 64, body mass = 72.0 ± 7.1 kg, body height = 182.1 ± 5.2 cm, age = 20.8 ± 4.0 y) with a 400-m personal best above 50 s. The power of the countermovement and the sequential squat jumps was measured in two sets after a warm-up, followed by two intermittent 30-s Wingate tests. LA concentration was measured eight times. It was observed that elite athletes achieved significantly higher power in both types of jumps. The maximum post-exercise LA concentration was significantly lower in the sub-elite group after the 3rd, the 6th, the 9th, and the 20th min after the cessation of two Wingate tests (p < 0.001). The rate of LA accumulation after exercise and the rate of LA utilization did not differ between the groups. It can be concluded that elite and non-elite runners differ in higher LA production but not in LA utilization. Anaerobic power and LA concentration seem to differentiate between 400 elite and sub-elite performance.

Genetic Association of MSTN Gene Variant (18:66493737T&gt;C) with Track Performance &amp; Muscle Development in Pakistani Horses

The horse, revered for its diverse traits including racing prowess, gaitedness, and distinctive behavior, plays a pivotal role in various sports. Numerous studies have linked racing performance in horses to the MSTN gene across global populations. Objectives: To investigate the genetic variability of the 18:66493737T&gt;C variant in Pakistani random-bred horses. Methods: ARMS-PCR was employed where 24 horses sourced from UVAS equine clinic were genotyped. Results: Revealing a population distribution of 54% homozygous wild-type (TT), 41% heterozygous (TC), and 4% homozygous mutant (CC) at this locus. The alternative allele frequency within elite performers and control horses stood at 0.36 and 0.12, respectively. Application of the Chi-Square association test using the PLINK data toolset yielded a highly significant p-value of 7.832×10-6. Conclusions: This underscores significant genetic variability at the locus in the Pakistani horse population, aligning with global patterns. Future studies are advocated, incorporating racing performance data and encompassing diverse indigenous horse breeds with substantial sample sizes. Identification of subject markers can inform targeted breeding strategies, contributing to the enhancement and preservation of desirable traits across various horse breeds