Elevated Temperatures Research Articles

Selective Cleavage of Cβ-O-4 Bond for Lignin Depolymerization via Paired-Electrolysis in an Undivided Cell.

The cleavage of C-O bonds is one of the most promising strategies for lignin-to-chemicals conversion, which has attracted considerable attention in recent years. However, current catalytic system capable of selectively breaking C-O bonds in lignin often requires a precious metal catalyst and/or harsh conditions such as high-pressure H2 and elevated temperatures. Herein, we report a novel protocol of paired electrolysis to effectively cleave the Cβ-O-4 bond of lignin model compounds and real lignin at room temperature and ambient pressure. For the first time, "cathodic hydrogenolysis of Cβ-O-4 linkage" and "anodic C-H/N-H cross-coupling reaction" are paired in an undivided cell, thus the cleavage of C-O bonds and the synthesis of valuable triarylamine derivatives could be simultaneously achieved in an energy-effective manner. This protocol features mild reaction conditions, high atom economy, remarkable yield with excellent chemoselectivity, and feasibility for large-scale synthesis. Mechanistic studies indicate that indirect H* (chemical absorbed hydrogen) reduction instead of direct electron transfer might be the pathway for the cathodic hydrogenolysis of Cβ-O-4 linkage.

Exploring Phase Transition and Structural Complexity in the Mixed Cation Uranium Oxide CaUNb2O8.

The structures and high-temperature phase transition of CaUNb2O8 were studied in situ using synchrotron X-ray and neutron powder diffraction. Rietveld refinements provided an accurate description of the crystal structures of both the monoclinic fergusonite-type I2/b structure observed at room temperature and the tetragonal scheelite-type I41/a structure found at high temperatures. Bond valence sum analysis showed Nb5+ to be octahedrally coordinated in the monoclinic fergusonite-type structure, akin to other ANbO4 materials. Rietveld analysis of the variable temperature data allowed for the determination of accurate unit cell parameters and atomic coordinates, as well as revealing a reversible phase transition around ∼750 °C. The Nb-O bond distances display anomalous behavior, with a discontinuity in the longer Nb-O(1') distance coinciding with the phase transition suggestive of a reconstructive phase transition. Mode analysis identified the Γ2+ mode as the primary mode that drives the phase transition; this is linearly coupled to the induced spontaneous strain within the monoclinic fergusonite-type structure. Analysis of the temperature dependence of the Nb(z) positional parameter, as well as of the ϵ1-ϵ2 and ϵ6 strain parameters, showed that the phase transition is not strictly second order, with the critical exponent β ≠ 1/2. This study demonstrates the complex structural features of mixed cation metal oxides at elevated temperatures.

Unraveling the Solution Aggregation Structures and Processing Resiliency of High-Efficiency Organic Photovoltaic Blends.

The solution aggregation structure of conjugated polymers is crucial to the morphology and resultant optoelectronic properties of organic electronics and is of considerable interest in the field. Precise characterizations of the solution aggregation structures of organic photovoltaic (OPV) blends and their temperature-dependent variations remain challenging. In this work, the temperature-dependent solution aggregation structures of three representative high-efficiency OPV blends using small-angle X-ray/neutron scattering are systematically probed. Three cases of solution processing resiliency are elucidated in state-of-the-art OPV blends. The exceptional processing resiliency of high-efficiency PBQx-TF blends can be attributed to the minimal changes in the multiscale solution aggregation structure at elevated temperatures. Importantly, a new parameter, the percentage of acceptors distributed within polymer aggregates (Ф), for the first time in OPV blend solution, establishes a direct correlation between Ф and performance is quantified. The device performance is well correlated with the Kuhn length of the cylinder related to polymer aggregates L1 at the small scale and the Ф at the large scale. Optimal device performance is achieved with L1 at ≈30nm and Ф within the range of 60±5%. This study represents a significant advancement in the aggregation structure research of organic electronics.

Designing a Novel Temperature- and Noncanonical Amino Acid-Controlled Biological Logic Gate in Escherichia coli.

Genetic code expansion (GCE) is a powerful strategy that expands the genetic code of an organism for incorporating noncanonical amino acids into proteins using engineered tRNAs and aminoacyl-tRNA synthetases (aaRSs). While GCE has opened up new possibilities for synthetic biology, little is known about the potential side effects of exogenous aaRS/tRNA pairs. In this study, we investigated the impact of exogenous aaRS and amber suppressor tRNA on gene expression in Escherichia coli. We discovered that in DH10β ΔcyaA, transformed with the F1RP/F2P two-hybrid system, the high consumption rate of cellular adenosine triphosphate by exogenous aaRS/tRNA at elevated temperatures induces temperature sensitivity in the expression of genes regulated by the cyclic AMP receptor protein (CRP). We harnessed this temperature sensitivity to create a novel biological AND gate in E. coli, responsive to both p-benzoylphenylalanine (BzF) and low temperature, using a BzF-dependent variant of E. coli chorismate mutase and split subunits of Bordetella pertussis adenylate cyclase. Our study provides new insights into the unexpected effects of exogenous aaRS/tRNA pairs and offers a new approach for constructing a biological logic gate.

Mixed-Matrix Organo-Silica–Hydrotalcite Membrane for CO2 Separation Part 1: Synthesis and Analytical Description

Hydrotalcite exhibits the capability to adsorb CO2 at elevated temperatures. High surface area and favorable coating properties are essential to harness its potential for practical applications. Stable alcohol-based dispersions are needed for thin film applications of mixed membranes containing hydrotalcite. Currently, producing such dispersions without the need for delamination and dispersing agents is a challenging task. This work introduces, for the first time, a manufacturing approach to overcoming the drawbacks mentioned above. It includes a synthesis of hydrotalcite nanoparticles, followed by agent-free delamination of their layers and final dispersion into alcohol without dispersing agents. Further, the hydrotalcite-derived sorption agent is dispersed in a matrix based on organo-silica gels derived from 1,2-bis(triethoxysilyl)ethane (BTESE). The analytical results indicate that the interconnection between hydrotalcite and BTESE-derived gel occurs via forming a strong hydrogen bonding system between the interlayer species (OH groups, CO32−) of hydrotalcite and oxygen and silanol active gel centers. These findings lay the foundation for applications involving incorporating hydrotalcite-like compounds into silica matrices, ultimately enabling the development of materials with exceptional mass transfer properties. In part 2 of this study, the gas separation performance of the organo-silica and the hydrotalcite-like materials and their combined form will be investigated.

Factors influencing seasonal chemistry patterns in Virginia mountain streams

AbstractThe relative influence of seasonal patterns in hydrological flow and seasonal differences in biological and geochemical activity on stream chemistry patterns is difficult to discern because they covary; temperate systems are characterized by lower mean flow in the summer (i.e. corresponding to deeper flow paths, elevated temperature, and biological activity), and higher mean flow in the winter (i.e. corresponding to shallower flow paths, depressed temperature, and biological dormancy). Using 2018 data, when seasonal stream flow conditions reversed, and two prior conventional water years, the relationship between monthly acid-relevant analyte concentrations and streamflow were compared within and between winter and summer to provide insight into controls on characteristic seasonal chemistry patterns at two mid-Appalachian sites with distinct geology (weatherable mafic and weather resistant siliciclastic). Acid neutralizing capacity (ANC) increased (1) with lower flow, in both seasons and (2) in summer, for all flow conditions. The compounding impacts resulted in a doubling of concentration from typical winter with high flow to summer with low flow at both sites. Base cation patterns tracked ANC at the mafic site, resulting in an ~ 60% increase of from winter with high flow to summer with low flow; distinctions between summer and winter contributed more to the seasonal pattern (72%) than changes in flow. Sulfate increased at the mafic site (1) with higher flow, in both seasons and (2) in winter, for all flow conditions, resulting in an ~ 50% increase from summer with low flow to winter with high flow; distinctions between winter and summer conditions and flow contributed similarly (40–60%) to the typical seasonal chemical pattern. The biogeochemical mechanism driving differences in stream chemistry between summer and winter for the same flow conditions is likely increased rates of natural acidification from elevated soil respiration in summer, resulting in greater bedrock weathering and sulfate adsorption. Findings highlight the significance and consistency of growing vs dormant season variations in temperature and biological activity in driving intra-annual patterns of stream solutes. This data set informs parameterization of hydro-biogeochemical models of stream chemistry in a changing climate at a biologically relevant, seasonal, timescale.

Geodynamic controls on clastic-dominated base metal deposits

Abstract. To meet the growing global demand for metal resources, new ore deposit discoveries are required. However, finding new high-grade deposits, particularly those not exposed at the Earth's surface, is very challenging. Therefore, understanding the geodynamic controls on the mineralizing processes can help identify new areas for exploration. Here we focus on clastic-dominated Zn–Pb deposits, the largest global resource of zinc and lead, which formed in sedimentary basins of extensional systems. Using numerical modelling of lithospheric extension coupled with surface erosion and sedimentation, we determine the geodynamic conditions required to generate the rare spatiotemporal window where potential metal source rocks, transport pathways, and host sequences are present. We show that the largest potential metal endowment can be expected in narrow asymmetric rifts, where the mineralization window spans about 1–3 Myr in the upper ∼ 4 km of the sedimentary infill close to shore. The narrow asymmetric rift type is characterized by rift migration, a process that successively generates hyper-extended crust through sequential faulting, resulting in one wide and one narrow conjugate margin. Rift migration also leads to (1) a sufficient life span of the migration-side border fault to accommodate a thick submarine package of sediments, including coarse (permeable) continental sediments that can act as source rock; (2) rising asthenosphere beneath the thinned lithosphere and crust, resulting in elevated temperatures in these overlying sediments that are favourable for leaching metals from the source rock; (3) the deposition of organic-rich sediments that form the host rock at shallower burial depths and lower temperatures; and (4) the generation of smaller faults that cut the major basin created by the border fault and provide additional pathways for focused fluid flow from source to host rock. Wide rifts with rift migration can have similarly favourable configurations, but these occur less frequently and less potential source rock is produced, thereby limiting potential metal endowment. In simulations of narrow symmetric rifts, the conditions to form ore deposits are rarely fulfilled. Based on these insights, exploration programmes should prioritize the narrow margins formed in asymmetric rift systems, in particular regions within several tens of kilometres from the paleo-shoreline, where we predict the highest-value deposits to have formed.

Effects of Heat Treatment on the Chemical Composition and Microstructure of Cupressus funebris Endl. Wood

The effects of heat treatment on Cupressus funebris Endl. wood were examined under different combinations of temperature, time, and pressure. The chemical composition, crystallinity, and microstructure of heat-treated wood flour and specimens were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Vacuum heat treatment led to changes in the functional groups and microstructure of C. funebris wood, and the relative lignin content decreased with increasing treatment temperature, which was significant at lower negative pressures. Cellulose crystallinity showed a change rule of first increasing and then decreasing throughout the heat treatment range, and the relative crystallinity ranged from 102.46% to 116.39%. The cellulose treated at 120 °C for 5 h at 0.02 MPa had the highest crystallinity of 44.65%. These results indicate that although heat treatment can improve cellulose crystallinity, very high temperatures can lead to decreased crystallinity. The morphology and structure of the cell wall remained stable throughout the heat treatment range; however, at elevated temperatures, slight deformation occurred, along with rupture of the intercellular layer.

High-temperature capacitive energy stroage in polymer nanocomposites through nanoconfinement

Polymeric-based dielectric materials hold great potential as energy storage media in electrostatic capacitors. However, the inferior thermal resistance of polymers leads to severely degraded dielectric energy storage capabilities at elevated temperatures, limiting their applications in harsh environments. Here we present a flexible laminated polymer nanocomposite where the polymer component is confined at the nanoscale, achieving improved thermal-mechanical-electrical stability within the resulting nanocomposite. The nanolaminate, consisting of nanoconfined polyetherimide (PEI) polymer sandwiched between solid Al2O3 layers, exhibits a high energy density of 18.9 J/cm3 with a high energy efficiency of ~ 91% at elevated temperature of 200°C. Our work demonstrates that nanoconfinement of PEI polymer results in reduced diffusion coefficient and constrained thermal dynamics, leading to a remarkable increase of 37°C in glass-transition temperature compared to bulk PEI polymer. The combined effects of nanoconfinement and interfacial trapping within the nanolaminates synergistically contribute to improved electrical breakdown strength and enhanced energy storage performance across temperature range up to 250°C. By utilizing the flexible ultrathin nanolaminate on curved surfaces such as thin metal wires, we introduce an innovative concept that enables the creation of a highly efficient and compact metal-wired capacitor, achieving substantial capacitance despite the minimal device volume.

Membranes Based on Aminated Graphene Oxide with High Selectivity Toward Organic Substances.

In this work, membranes based on graphene oxide, modified with oleylamine, have been prepared by a simple wet chemistry protocol without the use of complex equipment, elevated temperature, and additional reagents. The membrane material was characterized by a set of physicochemical methods: thermogravimetric analysis, Fourier transform infrared spectroscopy, X-ray diffractometry, and X-ray photoelectron spectroscopy. The prepared membranes are stable in both aqueous and organic media. The membranes have a high flux for organic substances and do not permeate water at room temperature and atmospheric pressure. The selectivity of the membranes toward organic substances increases with their thickness. The highest flux among the tested organic liquids is registered for methanol. The membranes have high selectivity toward ethanol/1-butanol and acetone/1-butanol pairs, which opens up the possibility of separating actual industrial mixtures. The membrane retains 90% of methylene blue from the alcohol solution. Our work expands the possibilities of using modified GO-based membranes in purification and filtration technologies.

Electronic cooling and energy harvesting using ferroelectric polymer composites

Thermal management emerges as a grand challenge of next-generation electronics. Efforts to develop compact, solid-state cooling devices have led to the exploration of the electrocaloric effect of ferroelectric polymers. Despite recent advances, the applications of electrocaloric polymers on electronics operating at elevated temperatures remain essentially unexplored. Here, we report that the ferroelectric polymer composite composed of highly-polarized barium strontium titanate nanofibers and electron-accepting [6,6] phenyl-C61-butyric acid methyl ester retains fast electrocaloric responses and stable cyclability at elevated temperatures. We demonstrate the effectiveness of electrocaloric cooling in a polymer composite for a pyroelectric energy harvesting device. The device utilizes a simulated central processing unit (CPU) as the heat source. Our results show that the device remains operational even when the CPU is overheated. Furthermore, we show that the composite functions simultaneously as a pyroelectric energy converter to harvest thermal energy from an overheated chip into electricity in the electrocaloric process. This work suggests a distinct approach for overheating protection and recycling waste heat of microelectronics.

Machine‐learning interatomic potentials for pyrolysis of polysiloxanes and properties of SiCO ceramics

AbstractWe present machine‐learning interatomic potentials (MLIPs) for simulations of Si–C–O–H compounds. The MLIPs are constructed from moment tensor potentials (MTPs) and were trained to a library of configurations that included polysiloxane structures, hypothetical crystalline and amorphous SiCOH structures, and trajectories of Si–C–O–H systems obtained via ab initio molecular dynamic (aiMD) simulations at elevated temperatures. Passive, active, and hybrid learning strategies were implemented to develop the MLIPs. The MLIPs reproduce vibrational properties of polymers and SiCOH structures obtained from aiMD simulations, thus providing a tool to identify chemical units and distinct structural characteristics through their vibrational properties. Simulations of the polymer‐to‐ceramic transformation show the development of mixed tetrahedra in SiCO ceramics and align with experimental observations. Million‐atom simulations for several nanoseconds highlight the precipitation of graphitic nanosheets from a carbon‐rich SiCO precursor. Atomistic simulations with the MLIPs deliver details of chemical reaction mechanisms during the pyrolysis of polysiloxanes, including methane abstraction and Kumada‐like rearrangements that transform the siloxane backbone. While the MLIPs still leave room for systematic improvement, they deliver simulations with “density functional theory (DFT)‐like” quality at low and high temperatures.

Evidence for phosphate-dependent control of symbiont cell division in the model anemone Exaiptasia diaphana.

The corals responsible for building tropical reefs are disappearing at an alarming rate as elevated sea temperatures cause them to bleach and lose the algal symbionts they rely on. Without these symbionts, corals are unable to harvest energy from sunlight and, therefore, struggle to thrive or even survive in the nutrient-poor waters of the tropics. To devise solutions to address the threat to coral reefs, it is necessary to understand the cellular events underpinning the bleaching process. One model for bleaching proposes that heat stress impairs algal photosynthesis and transfer of sugar to the host. Consequently, the host's demands for nitrogen decrease, increasing nitrogen availability to the symbionts, which leads to an increase in algal proliferation that overwhelms the host. Our work suggests that phosphate may play a similar role to nitrogen in this feedback loop.

Design of Cr-Based Molecular Electrocatalyst Systems for the CO2 Reduction Reaction.

ConspectusHuman influence on the climate system was recently summarized by the sixth Intergovernmental Panel on Climate Change (IPCC) Assessment Report, which noted that global surface temperatures have increased more rapidly in the last 50 years than in any other 50-year period in the last 2000 years. Elevated global surface temperatures have had detrimental impacts, including more frequent and intense extreme weather patterns like flooding, wildfires, and droughts. In order to limit greenhouse gas emissions, various climate change policies, like emissions trading schemes and carbon taxes, have been implemented in many countries. The most prevalent anthropogenic greenhouse gas emitted is carbon dioxide (CO2), which accounted for 80% of all U.S. greenhouse gas emissions in 2022. The reduction of CO2 through the use of homogeneous electrocatalysts generally follows a two-electron/two-proton pathway to produce either carbon monoxide (CO) with water (H2O) as a coproduct or formic acid (HCOOH). These reduced carbon species are relevant to industrial applications: the Fischer-Tropsch process uses CO and H2 to produce fuels and commodity chemicals, while HCOOH is an energy dense carrier for fuel cells and useful synthetic reagent. Electrochemically reducing CO2 to value-added products is a potential way to address its steadily increasing atmospheric concentrations while supplanting the use of nonrenewable petrochemical reserves through the generation of new carbon-based resources. The selective electrochemical reduction of CO2 (CO2RR) by homogeneous catalyst systems was initially achieved with late (and sometimes costly) transition metal active sites, leading the field to conclude that transition metal complexes based on metals earlier in the periodic table, like chromium (Cr), were nonprivileged for the CO2RR. However, metals early in the table have sufficient reducing power to mediate the CO2RR and therefore could be selective in the correct coordination environment. This Account describes our efforts to develop and optimize novel Cr-based CO2RR catalyst systems through redox-active ligand modification strategies and the use of redox mediators (RMs). RMs are redox-active molecules which can participate cocatalytically during an electrochemical reaction, transferring electrons─often accompanied by protons─to a catalytic active site. Through mechanistic and computational work, we have found that ligand-based redox activity is key to controlling the intrinsic selectivity of these Cr compounds for CO2 activation. Ligand-based redox activity is also essential for developing cocatalytic systems, since it enables through-space interactions with reduced RMs containing redox-active planar aromatic groups, allowing charge transfer to occur within the catalyst assembly. Following a summary of our work, we offer a perspective on the possibilities for future development of catalytic and cocatalytic systems with early transition metals for small molecule activation.

Utilization of Polycyclic Aromatic Solid Additives for Morphology and Thermal Stability Enhancement in Photoactive Layers of Organic Solar Cells.

Volatile solid additives have emerged as a promising strategy for enhancing film morphology and promoting the power conversion efficiency (PCE) of organic solar cells (OSCs). Herein, a series of novel polycyclic aromatic additives with analogous chemical structures, including fluorene (FL), dibenzothiophene (DBT), and dibenzofuran (DBF) derived from crude oils, are presented and incorporated into OSCs. All these additives exhibit strong interactions with the electron-deficient terminal groups of L8-BO within thebulk-heterojunction OSCs. Moreover, they demonstrate significant sublimation during thermal annealing, leading to increase free volumes for the rearrangement and recrystallization of L8-BO. This phenomenon leads to an improved film morphology and an elevated glass-transition temperature of the photoactive layers. Consequently, the PCE of the PM6:L8-BO blend has been boosted from 16.60% to 18.60% with 40 wt% DBF additives, with a champion PCE of 19.11% achieved for ternary PM6:L8-BO:BTP-eC9 OSCs. Furthermore, the prolonged shelf and thermal stability have been observed in OSCs with these additives. This study emphasizes the synergic effect of volatile solid additives on the performance and thermal stability of OSCs, highlighting their potential for advancing the field of photovoltaics.

Effect of a novel high‐fibre component from quinoa on the properties of bread‐making

SummaryThe study investigates the impact of incorporating a fibre‐rich component derived from quinoa (QFC) into wheat flour on the physicochemical, rheological and bread‐making characteristics. QFC, possessing triple the dietary fibre contents of quinoa grain, increased fibre levels in blends from 4% to 12%. As QFC inclusion increased from 0% to 20%, farinograph water absorption increased from 59.6% to 66.7%, while dough stability decreased from 9.9 to 3.5 min. Amylograph properties exhibited elevated gelatinisation temperature and reduced viscosities with QFC supplementation. Bread hardness surged from 997 to 2800 g with 0%–20% QFC incorporation. Sensory quality remained acceptable up to 15% QFC, beyond which it significantly declined. Additives enhanced bread volume from 2.66 to 3.34 for 15% QFC bread, along with sensory parameters. The QFC‐incorporated bread not only improved nutritional quality but also positioned itself as a viable fibre‐rich alternative in the food industry, promising benefits for health‐conscious consumers.

Pressure-Induced Enhancement in Chemical Looping Reforming of CH4: A Thermodynamic Analysis with Fe-Based Oxygen Carriers.

Chemical looping reforming of methane (CLRM) with Fe-based oxygen carriers is widely acknowledged as an environmentally friendly and cost-effective approach for syngas production, however, sintering-caused deactivate of oxygen carriers at elevated temperatures of above 900 °C is a longstanding issue restricting the development of CLRM. Here, in order to reduce the reaction temperature without compromising the chemical-looping CH4 conversion efficiency, we proposed a novel operation scheme of CLRM by manipulating the reaction pressure to shift the equilibrium of CH4 partial oxidation towards the forward direction based on the Le Chatelier's principle. The results from thermodynamic simulations showed that, at a fixed reaction temperature, the reduction in pressure led to the increase in CH4 conversion, H2 and CO selectivity, as well as carbon deposition rate of all investigated oxygen carriers. The pressure-negative CLRM with Fe3O4, Fe2O3 and MgFe2O4 could reduce the reaction temperature to below 700 °C on the premise of a satisfactory CLRM performance. In a comprehensive consideration of the CLRM performance, energy consumption, and CH4 requirement, NiFe2O4 was the Fe-based OCs best available for pressure-negative CLRM, especially for an excellent syngas yield of 23.08 mmol/gOC. This study offered a new strategy to address sintering-caused deactivation of materials in chemical looping from the reaction thermodynamics point of view.

Micro-Structure Engineering in Pd-InOx Catalysts and Mechanism Studies for CO2 Hydrogenation to Methanol

Significant interest has emerged for the application of Pd-In2O3 catalysts as high-performance catalysts for CO2 hydrogenation to CH3OH. However, precise active site control in these catalysts and understanding their reaction mechanisms remain major challenges. In this investigation, a series of Pd-InOx catalysts were synthesized, revealing three distinct types of active sites: In-O, Pd-O(H)-In, and Pd2In3. Lower Pd loadings exhibited Pd-O(H)-In sites, while higher loadings resulted in Pd2In3 intermetallic compounds. These variations impacted catalytic performance, with Pd-O(H)-In catalysts showing heightened activity at lower temperatures due to the enhanced CO2 adsorption and H2 activation, and Pd2In3 catalysts performing better at elevated temperatures due to the further enhanced H2 activation. In situ DRIFTS studies revealed an alteration in key intermediates from *HCOO over In-O bonds to *COOH over Pd-O(H)-In and Pd2In3 sites, leading to a shift in the main reaction pathway transition and product distribution. Our findings underscore the importance of active site engineering for optimizing catalytic performance and offer valuable insights for the rational design of efficient CO2 conversion catalysts.

The Effect of Artificial Ageing on the Changes in Selected Properties of Organic Coated Sheets

This contribution presents the results of research focused on changes in selected properties of organic-coated steel sheets in a corrosive environment of salt mist. The aim of this study was to characterise coated sheets and to analyse the influence of elevated temperature on changes in their selected properties. The methodology and experiments were chosen to simulate the accelerated ageing and to evaluate the surface of organic-coated steel sheets. During the research, the effect of artificial ageing due to increased temperature on changes in mechanical properties of coated sheets was monitored, which were determined by tensile testing according to STN EN 10002-1. The fracture surface of the test samples was analysed using a SEM. The following observations were made for the evaluation of coating by cross-cut tests according to EN ISO 2409. Corrosion tests were carried out by conducting salt spray corrosion tests according to EN ISO 9227. The test specimens were artificially sectioned using a laser beam. The results of the research experiments confirmed the assumption that the utilisation of the laser beam was convenient for the creation cross–cut test of the material. After the ageing test, only small significant changes in the basic mechanical properties of the tested materials were observed. It is necessary to take into account that there were changes in the appearance of the coating. The cross-cut test confirmed the results that the plastic-coated sheets could be utilised in salt corrosion environments.

Flavonols improve tomato pollen thermotolerance during germination and tube elongation by maintaining ROS homeostasis.

Elevated temperatures impair pollen performance and reproductive success, resulting in lower crop yields. The tomato (Solanum lycopersicum) anthocyanin reduced (are) mutant harbors a mutation in FLAVANONE 3-HYDROXYLASE (F3H), resulting in impaired flavonol antioxidant biosynthesis. The are mutant has reduced pollen performance and seed set relative to the VF36 parental line, phenotypes that are accentuated at elevated temperatures. Transformation of are with the wild-type F3H gene, or chemical complementation with flavonols, prevented temperature-dependent reactive oxygen species (ROS) accumulation in pollen and restored the reduced viability, germination, and tube elongation of are to VF36 levels. Overexpression of F3H in VF36 prevented temperature-driven ROS increases and impaired pollen performance, revealing that flavonol biosynthesis promotes thermotolerance. Although stigmas of are had reduced flavonol and elevated ROS levels, the growth of are pollen tubes was similarly impaired in both are and VF36 pistils. RNA-seq was performed at optimal and stress temperatures in are, VF36, and the F3H overexpression line at multiple timepoints across pollen tube elongation. The number of differentially expressed genes increased over time under elevated temperatures in all genotypes, with the greatest number in are. These findings suggest potential agricultural interventions to combat the negative effects of heat-induced ROS in pollen that lead to reproductive failure.