The current study was carried out during the period from January 2017 to June 2017 in Maysan/Amareh city for the detection of parasitic children infected with Entamoeba histolytica. One hundred child patients at the age's group between 1–10 years old from both males and females with acute diarrhea clinically presented with bloody and mucoid diarrhea used to participate in the study. Samples collected from patient's (clinic paediatric in Shaheed Al-Sadr Teaching Hospital/Iraq, Al Zahrawi Surgical Hospitaland from the private laboratories City of Ammaraa) and 25 persons as a healthy control. The results of biochemical parameters showed an elevated levels in liver the enzymes (GGT) and (LHD) was significantly higher than that observed in the non-patient (P<0.05). According to serum electrolytes, the serum level for, ionized calcium, and sodium were significantly lower for patients group (P<0.05). While for phosphorus and potassium there's no significant difference between the patients group. The PH value for patients group (7.85) was significantly higher than non-patient (control group) (7.46).