Elevated Concentrations Of Cd Research Articles

Cadmium Associated Preeclampsia: A Systematic Literature Review of Pregnancy and Birth Outcomes.

Preeclampsia (PE), caused by multiple factors, is one of the most serious complications of pregnancy. Cadmium (Cd) is a heavy metal environmental pollutant, reproductive toxicant, and endocrine disruptor, which can increase the risk of PE. Cd toxicity due to occupational, diet, and environmental factors has worsened the risk. Studies showed elevated Cd concentration in maternal blood and placenta of PE women. However, the implicit association between Cd associated PE is still not highlighted. We systematically reviewed Cd-associated PE and its effect on pregnancy and birth outcomes. Based on "Preferred reporting items for systematic reviews and meta-analyses (PRISMA)" guidelines, eighty-six studies were identified by PubMed, Web of Science (WOS), and Scopus databases. Publications were included until October 2023 and articles screened based on our inclusion criteria. Our study identified that the exposure of controlled and uncontrolled Cd induces PE, which negatively affects pregnancy and birth outcomes. Given the serious nature of this finding, Cd is a potential adverse agent that impacts pregnancy and future neonatal health. Further comprehensive studies covering the whole trimesters of pregnancy and neonatal developments are warranted. Data on the molecular mechanisms behind Cd-induced PE is also essential for potential preventive, diagnostic, or therapeutic targets.

Health risk assessment of potentially toxic elements in the riparian zone of the Sava River (southeastern Europe): effects of high and low water events

This study evaluated the source apportionment and level of contamination with potential toxic elements (PTEs)—As, Cd, Cr, Cu, Ni, Pb and Zn—in a riparian zone of the Sava River by calculating the Enrichment Factor (EF) and the Geo-Accumulation Index (Igeo). The study analysed the non-carcinogenic and carcinogenic health risks to both adults and children in the local population. Soil and sediment samples collected during high and low water events from 12 sites along 945 km of the river were screened. Elevated soil contents of As, Cr, Cu, Ni, and Zn were observed along the middle and lower stretches from Županja (ZUP) to Belgrade (BEO), and an elevated Cu content was detected in the upper stretch Čatež (CAT), highlighting these elements as a potential risk for the human population. Elevated As, Cd Cr, Ni, and Zn levels in the sediment were also detected, with the Ni content three times higher than the Permissible Exposure Limit (PEL) values, indicating high contamination. The study established that As, Cr, and Ni primarily originate from geological sources; Cu, Pb, and Zn are predominantly from anthropogenic sources; and Cd originates from both geological and anthropogenic sources. Although the EF and Igeo varied along the entire river stretch, they were significantly higher for most elements in the lower stretch, indicating intensive anthropogenic contamination and potential health risks. The carcinogenic and non-carcinogenic risks associated with soils and sediments during high and low water events did not imply a potential risk for adults. However, As, Cd, Cr, and Pb potentially pose the greatest non-carcinogenic risk for children in both high and low water events, with ingestion as the main route of exposure. According to our findings, the elevated concentrations of As, Cd, Cr, and Pb in the Sava River basin (SRB) pose a significant potential health risk to the local population based on several factors: the high levels of these elements, their potential contamination risk as determined by the EF and Igeo, and their bioavailability and solubility. Although the cumulative Total Hazard Index (THI) and Total Carcinogenic Risk (TCR) values are low, particularly in the lower stretch of the Sava River, these elements still present a high potential health threat.

Trace metal accumulation through the environment and wildlife at two derelict lead mines in Wales

Trace metal pollution is globally widespread, largely resulting from human activities. Due to the persistence and high toxicity of trace metals, these pollutants can have serious effects across ecosystems. However, few studies have directly assessed the presence and impact of trace metal pollution across ecosystems, specifically across multiple environmental sources and animal taxa. This study was designed to assess the environmental health impacts of trace metal pollution by assessing its extent and possible transfer into wildlife in the areas surrounding two abandoned metalliferous mine complexes in Wales in the UK. Water, sediment, and soil at the mine sites and in areas downstream had notably elevated concentrations of Pb, Zn, and, to a lesser extent, Cd and Cu, when compared to nearby control sites. These high trace metal concentrations were mirrored in the body burdens of aquatic invertebrates collected in the contaminated streams both at, and downstream of, the mines. Wood mice collected in contaminated areas appeared to be able to regulate their Zn and Cu tissue concentrations, but, when compared to wood mice from a nearby control site, they had significantly elevated concentrations of Cd and, particularly, Pb, detected in their kidney, liver, and bone samples. The Pb concentrations found in these tissues correlated strongly with local soil concentrations (kidney: ρ = 0.690; liver: ρ = 0.668, bone: ρ = 0.649), and were potentially indicative of Pb toxicity in between 10 % and 82 % of the rodents sampled at the mine sites and in areas downstream. The high trace metal concentrations found in the environment and in common prey species (invertebrates and rodents) indicates that trace metal pollution can have far-reaching, ecosystem-wide health impacts long after the polluting activity has ceased, and far beyond the originating site of the pollution.

Assessment of Nutrients and Heavy Metals in the Sediments of the Dali River and Wuding River

ABSTRACT Low ecological risk from these parameters. However, cadmium (Cd), mercury (Hg), and zinc (Zn) exhibited relatively high enrichment factors and geo-accumulation indices, suggesting potential contamination from anthropogenic sources. Potential ecological risk analysis indicated a very high ecological risk associated with heavy metal concentrations in the sediments of Dali River and Wuding River. Statistical analysis revealed strong correlations between metal (Ni, Cd, Cu, and Cr) concentrations and TOC, suggesting that nutrients and those heavy metals might have common pollution sources and similar transport behavior. Principal component analysis identified elevated Cd, Cu, and Ni concentrations as potentially originating from OM, while As may be derived from municipal sewage. Hg and Zn could be probably attributed to industrial effluents. The findings of this study highlight the need for effective pollution control measures to mitigate the ecological risks posed by heavy metal contamination in the sediments of Dali River and Wuding River.

Phytoremediation Potential of Flax Grown on Multimetal Contaminated Soils: A Field Experiment.

The aim of this study was to assess the phytoremediation potential of fiber flax (Linum usitatissimatum L., var. Calista) cultivated in a soil contaminated with multiple metals, under real field conditions. A two-year (2022 and 2023) field experiment was conducted in a site contaminated with elevated concentrations of Cd, Ni, Cu, Pb, and Zn due to mining and metallurgical activities. Three different nitrogen fertilization levels were tested (N0: 0 kg N ha-1, N1: 30 kg N ha-1, N2: 60 kg N ha-1), and both spring and winter sowings were conducted. At full maturity, growth parameters and yields were measured. The phytoremediation potential of flax was assessed in terms of the metal concentrations in the above-ground biomass and of the metal uptake (i.e., the potential removal of the soil metals in g ha-1 and per year). Flax demonstrated a shorter growth cycle, with shorter and thicker plants and higher yields when sown in spring compared to winter sowing. Plant growth and productivity were not evidently influenced by additional nitrogen fertilization during plant growth. The cadmium bioaccumulation factor was 1.06, indicating that flax accumulates this metal. For Ni, Cu, Pb, and Zn, the corresponding values were 0.0, 0.04, 0.004, and 0.02, suggesting that this crop excludes these metals. The order of the higher uptake in plant tissues was as follows: Zn > Pb > Cd > Cu > Ni. In conclusion, flax demonstrated tolerance to heavy metals in the soil, effectively supporting soil restoration through cultivation. Additionally, flax showed potential as a cadmium accumulator while excluding nickel, copper, lead, and zinc.

Genome-wide identification of bHLH gene family and its response to cadmium stress in Populus × canescens.

The basic helix-loop-helix (bHLH) gene family is integral to various aspects of plant development and the orchestration of stress response. This study focuses on the bHLH genes within Populus × canescens, a poplar species noted for its significant tolerance to cadmium (Cd) stress. Through our comprehensive genomic analysis, we have identified and characterized 170 bHLH genes within the P. canescens genome. These genes have been systematically classified into 22 distant subfamilies based on their evolutionary relationships. A notable conservation in gene structure and motif compositions were conserved across these subfamilies. Further analysis of the promoter regions of these genes revealed an abundance of essential cis-acting element, which are associated with plant hormonal regulation, development processes, and stress response pathway. Utilizing quantitative PCR (qPCR), we have documented the differential regulation of PcbHLHs in response to elevated Cd concentrations, with distinct expression patterns observed across various tissues. This study is poised to unravel the molecular mechanism underpinning Cd tolerance in P. canescens, offering valuable insights for the development of new cultivars with enhanced Cd accumulation capacity and tolerance. Such advancements are crucial for implementing effective phytoremediation strategies to mitigate soil pollution caused by Cd.

Vertical distribution and contamination assessment of heavy metals in the vadose zone of a 50-year-old smelter dumpsite, San Antonio Bay, Argentina

This research aimed to assess the contamination levels and mineralogical association of heavy metals in the vadose zone beneath smelter residues that were freely deposited over natural sediments from the 1960 s to the 1980 s. The results revealed elevated concentrations of heavy metals, particularly Fe, Pb, Zn, Cu, and Cd. The highest concentrations were found within the superficial layer, corresponding to the smelter slag (203,703 – 219,998 mg kg−1 Fe; 73,708 – 136,390 mg kg−1 Pb; 18,922 – 29,484 mg kg−1 Zn; 6473 – 7887 mg kg−1 Cu; 20 – 48 mg kg−1 Cd). This residue is an acidic material mainly constituted by secondary sulfates and oxyhydroxides. Although concentrations decreased with depth, the levels of these metals remained higher than the background even at depths of 300 cm. Precipitation of secondary minerals (plumbojarosite, anglesite, Zn-sulfates, and Fe oxydroxides) from the percolating leachates was detected in the vadose zone based on SEM and EDS analysis. According to their vertical distribution, Cd and Zn showed a higher mobility than Cu, Fe and Pb, which were primarily concentrated at the surface layer. The geo-accumulation index (Igeo) was Pb > Cu > Zn > Cd > Ni. The vadose zone is severely polluted and represents a very high risk to the environment based on the ecological risk factor (Eri) and risk index (RI). The metals that contribute most to this condition are Pb > Cd ≈ Cu > Zn. Given the severity of the situation, remediation of the study area is recommended.

Potentially toxic elements (PTEs) and ecological risk at waste disposal sites: An analysis of sanitary landfills.

This study presents an analysis of soil contamination caused by Ni, Zn, Cd, Cu, and Pb at municipal solid waste (MSW) landfills, with a focus on ecological risk assessment. The approach aims to assess how different landfill practices and environmental conditions affect soil contamination with potentially toxic elements (PTEs) and associated environmental risks. Soil samples were collected from MSW landfills in Poland and the Czech Republic. The research included a comprehensive assessment of PTEs in soils in the context of global environmental regulations. The degree of soil contamination by PTEs was assessed using indices: Geoaccumulation Index (Igeo), Single Pollution Index (Pi), Nemerow Pollution Index (PN), and Load Capacity of a Pollutant (PLI). The ecological risk was determined using the Risk of PTEs (ERi) and Sum of Individual Potential Risk Factors (ERI). The maximum values of the indicators observed for the Radiowo landfill were as follows: Igeo = 4.04 for Cd, Pi = 24.80 for Cd, PN = 18.22 for Cd, PLI = 2.66, ERi = 744 for Cd, ERI = 771.80. The maximum values of the indicators observed for the Zdounky landfill were as follows: Igeo = 1.04 for Cu, Pi = 3.10 for Cu, PN = 2.52 for Cu, PLI = 0.27, ERi = 25 for Cd, ERI = 41.86. The soils of the tested landfills were considered to be non-saline, with electrical conductivity (EC) values less than 2,000 μS/cm. Varying levels of PTEs were observed, and geostatistical analysis highlighted hotspots indicating pollution sources. Elevated concentrations of Cd in the soil indicated potential ecological risks. Concentrations of Cu and lead Pb were well below the thresholds set by the environmental legislation in several countries. In addition, Ni concentrations in the soils of both landfills indicated that the average levels were within acceptable limits. Principal Component Analysis (PCA) revealed common sources of PTEs. The identification of specific risk points at the Radiowo and Zdounky sites contributes to a better understanding of potential hazards in landfill environments. By establishing buffer zones and implementing regular maintenance programs, emerging environmental problems can be addressed in a timely manner.

Metal accumulation and genetic adaptation of Oryza sativa to Cadmiun and Chromium heavy metal stress: A hydroponic and RAPD analyses

This research was performed to assess the influence of Cd and Cr metals on growth, pigments, antioxidant, and genomic stability of Oryza sativa indica and Oryza sativa japonica were investigated under hydroponic conditions. The results revealed that significant metal influence on test crop growth, pigment content, metal stress balancing antioxidant activity in a dose dependent manner. Since, while at elevated (500 ppm) concentration of Cd as well as Cr metals the pigment (total chlorophyll, chlorophyll a, b and carotenoids) level was reduced than control; however antioxidant activity (total antioxidant, H2O2, and NO) was considerably improved as protective mechanisms to combat the metal toxicity and support the plant growth. Furthermore, the test crops under typical hydroponic medium (loaded with Cd and Cr as 200, 300, 400, and 500 ppm) growth conditions, effectively absorb the metals from medium and accumulated in the root and least quantity was translocated to the shoot of this test crops. Furthermore, typical RAPD analysis with 10 universal primers demonstrated that the genomic DNA of the test crops was adaptable to develop metal resistance and ensure crop growth under increased concentrations (500 ppm) of tested heavy metals. These findings suggest that these edible crops have the ability to accumulate Cd along with Cr metals, and additionally that their genetic systems have the ability to adapt to metal-stressed environments.

Spatial Analysis of Cadmium and Lead in Soils of Nagaon District of Assam, India

Aims: The study was carried out in order to find the hotspots of Cadmium and Lead accumulation in the soil due to the anthropogenic activities, so that proper management strategies can be taken on time for their remediation.
 Study Design: Different geospatial techniques as Normal Q-Q plot, histogram, trend analysis and semi-variogram cloud model is used.
 Place and Duration of Study: The study was conducted emphasizing basically in high agricultural chemicals dependent Nagaon District of Assam, India, from 2018 to 2021.
 Methodology: Heavy metals were assessed using a random sampling method with a 5 x 5 km2 grid, and a total of 160 samples were collected from a depth of 0 – 15 cm for soil. Choropleth maps were created to show the distribution and hotspots of pollution.
 Results: Geospatial analyses from different thematic maps of heavy metals revealed significant vulnerable points of elevated concentrations of Pb (>24.45 mg/kg) and Cd (>0.31 mg/kg) in soil which is presumed to be due to anthropogenic factors. The three-dimensional trend over the distribution of metals throughout the district best fitted the second-order polynomial for Cd, and Pb in soils. Significant numbers of pairs of heavy metal pairs to a certain extent were found to be spatially autocorrelated and all the pairs away from X-axis towards the extreme right corner and far above the axis reflected less influence of local characteristics of the heavy metal. Co-variance cloud with search direction from North to South revealed the existence of spatial autocorrelation and a wider spatial shift of correlation towards the southern direction.
 Conclusion: The current study provides baseline data to update the mitigation approaches to better manage the heavy metal contamination in soil in the entire district.

Bioavailability, Sources, and Transfer Behavior of Heavy Metals in Soil–Crop Systems from a High Geological Background Area Impacted by Artisanal Zn Smelting in Guizhou Province, Southwest China

The environmental risk posed by heavy metals in agricultural soil is primarily influenced by their sources, bioavailability, and geochemical transfer behavior. This study focused on Weining County, a region in Guizhou province, Southwest China, with a high geological background and long-term impact from artisanal Zn smelting. Vertical soil profiles, crop, and rhizospheric soil samples were collected and analyzed for heavy metal concentration (As, Cd, Co, Cr, Cu, Hg, Ni, Pb, V, and Zn) including the total concentration and chemical fraction. The results revealed elevated concentrations of Cd (range: 0.7–6.9 mg·kg−1), Co (range: 19.3–120.0 mg·kg−1), Cu (range: 71.6–386.0 mg·kg−1), Ni (range: 51.0–121.0 mg·kg−1), and V (range: 310.0–721.0 mg·kg−1) in all soil samples compared to the background values of Guizhou Province. Chemical fractionation analysis indicated that Cr, Ni, As, Cu, and Zn were predominantly present in the residual fraction, while Hg and Pb were predominantly found in the potentially bioavailable fraction. Cd exhibited the highest bioavailability, accounting for 58.5% of its total concentration. Enrichment factor analysis suggested that artisanal Zn smelting activities were the main sources of Cd, Pb, and Zn contamination. Furthermore, Cd, Pb, and Zn were found to be highly accumulated in the surface soil layer (0–20 cm). Notably, 90.0% of potato and 9.4% of maize grain samples exceeded the food hygiene standards for Cd concentration, posing potential health risks to consumers. The bioconcentration factor (soil-to-root) and translocation factor (root-to-grain) analyses indicated that maize roots had a higher tendency to accumulate Cd from the soil, while Zn and Cu showed a significant transferability from roots to maize grains. These findings offer valuable insights for devising heavy metal remediation strategies in similar areas.

Cadmium accumulation and isotope fractionation in typical protozoa Tetrahymena: A new perspective on remediation of Cd pollution in wastewater

Cadmium (Cd) pollution threatens water safety and human health, which has raised serious public concern. Tetrahymena is a model protozoan, possessing the potential to remediate Cd contaminated water given the rapid expression of thiols. However, the mechanism of Cd accumulation in Tetrahymena has not been well understood, which hinders its application in environmental remediation. This study elucidated the pathway of Cd accumulation in Tetrahymena using Cd isotope fractionation. Our results showed that Tetrahymena preferentially absorb light Cd isotopes, with Δ114/110CdTetrahymena−solution = −0.20 ± 0.02‰ ∼ − 0.29 ± 0.02‰, which implies that the intracellular Cd is probably in the form of Cd−S. The fractionation generated by Cd complexation with thiols is constant (Δ114/110CdTetrahymena−remaining solution ∼ −0.28 ± 0.02‰), which is not affected by the concentrations of Cd in intracellular and culture medium, nor by the physiological changes in cells. Furthermore, the detoxification process of Tetrahymena results in an increase in cellular Cd accumulation from 11.7% to 23.3% with the elevated Cd concentrations in batch Cd stress culture experiments. This study highlights the promising application of Cd isotope fractionation in Tetrahymena for the remediation of heavy metal pollution in water.

Spatial prediction and influencing factors identification of potential toxic element contamination in soil of different karst landform regions using integration model

The prediction of contamination distribution of potentially toxic elements (PTEs) in soils of Guangxi province, China and the identification of their controlling factors pose great challenges due to diverse bedrock types, intense leaching and weathering, and discontinuous terrain distributions. Herein, we integrated the random forest (RF) and empirical Bayesian kriging (EBK) to interpret and predict complex PTEs contamination distribution from three different karst landform regions (fenglin, fengcong, isolated peak plain) in Guangxi province. The modeling results are compared with the commonly used ordinary kriging and regression-kriging. In this study, our developed RF-EBK model combines the advantages of the RF and EBK model to promote the prediction accurately and efficiently. In this study, it was shown that the integration RF-EBK model exhibited desirable for Cd and As concentrations, with R2 of 0.89 and 0.83, respectively. The average RMSE and MAE of integration RF-EBK model decreased by 39% and 44%, respectively, relative to the regression-kriging with the second highest accuracy. Furthermore, the modeling results showed that approximately 41.96% and 18.96% of total area was classified as Cd and As polluted and above regions (Igeo >0) in Guangxi province, respectively. Higher Cd concentration was observed in the soil of fenglin and fengcong regions than that in isolated peak plain region due to the secondary enrichment and parent rock inheritance, while the As concentration exhibited no significant difference among the three regions. The modeling results indicated that the elevated Cd concentration might be associated with soil CaO concentration and alkaline soil environment, whereas As concentration tended to be increased with the elevating Fe2O3 concentrations in weakly acidic soil environment. This result confirmed the applicability and effectiveness of integration model in predicting complex spatial patterns of soil PTEs and identifying their controlling factors.

Impact of past mining activities on water quality in a karst area in the Cévennes region, Southern France

Sampling and analysis of groundwater and surface water were conducted to assess the potential impacts of abandoned mines on water quality in a karst area in Southern France. The results of multivariate statistical analysis and geochemical mapping revealed that water quality is affected by contaminated drainage from abandoned mine sites. Acid mine drainage with very high concentrations of Fe, Mn, Al, Pb and Zn was identified in a few samples collected from mine openings and near waste dumps. In general, neutral drainage with elevated concentrations of Fe, Mn, Zn, As, Ni and Cd was observed due to buffering by carbonate dissolution. The contamination is spatially limited around abandoned mine sites, suggesting that metal(oid)s are sequestered in secondary phases that form under near-neutral and oxidizing conditions. However, the analysis of seasonal variations in trace metal concentrations showed that the transport of metal contaminants in water is highly variable according to hydrological conditions. During low flow conditions, trace metals are likely to be rapidly sequestered in Fe-oxyhydroxides and carbonate minerals in the karst aquifer and the river sediments, while low or no surface runoff in intermittent rivers limits the transport of contaminants in the environment. On the other hand, significant amounts of metal(loid)s can be transported under high flow conditions, primarily in dissolved form. Dissolved metal(loid) concentrations in groundwater remained elevated despite dilution by uncontaminated water, likely as a result of the increased leaching of mine wastes and the flushing of contaminated waters from mine workings. This work shows that groundwater is the main source of contamination to the environment and highlights the need to better understand the fate of trace metals in karst water systems.

Environmental impact of e-waste management in Indian microscale informal sectors.

Inappropriate e-waste processing in the informal sector is a serious issue in developing countries. Field investigations in microscale informal recycling sites have been performed to study the impact of hazardous metal(loid)s (released from e-waste dismantling) on the environment (water and soil). Eight hazardous metal(loid)s (Pb, Cd, Cu, Zn, As, Hg, Ni, and Cr) were primarily found in the monitored water and soil samples (Sangrampur, West Bengal) because of widespread informal e-waste handling and primitive processing. Elevated concentrations of Cd, Pb, As, Cu, and Cr were observed in pond water samples (0.04, 1.62, 0.03, 1.40, 1.74mg/L respectively). These ponds, which are regularly used for e-waste handling/dismantling, are usually flooded during the monsoon season mixing with further larger water resources - posing a serious threat to public health. Enriched levels of Pb, Cd, Cu, and Zn were detected in collected soil samples, both top surface soil (Pb up to 2042.27 ± 206.80, Cd up to 25.90 ± 9.53, Cu up to 6967.30 ± 711.70, and Zn up to 657.10 ± 67.05mg/Kg) and deeper subsurface soil (Pb, 419.70 ± 44.70; Cd, 18.34 ± 3.81; Cu, 3928.60 ± 356.40; and Zn, 134.40 ± 33.40mg/Kg), compared to the levels of As, Hg, Ni, and Cr. Seasonal variation of soil metal(loid) content indicated that higher levels of most of the metal(loids) were detected in the pre-monsoon (Nov-May) season, possibly due to the monsoonal dilution effect, except for Pb and Cd. The results highlighted that the composition and the handling of e-waste were important factors affecting the metal(loid) concentrations. E-waste policy and legislation have great influence on the handling and disposal procedures. An improved e-waste management practice has been proposed to encourage eco-friendly and safe e-waste disposal. It is recommended that regulatory agencies and manufacturers should create a road map to convince the informal sector to develop a systematic approach towards a more standardized formal e-waste management practices at the microscale field level.

Investigating the potential impacts of coal ash runoff on the freshwater Seminole ramshorn snail (Planorbella duryi) under laboratory conditions

Coal fly ash is an industrial waste product generated by coal fired powerplants which has been shown to contain elevated concentrations of several toxic trace metals. When stored in landfills or other repositories, these trace metals can enter nearby surface waters via a number of routes including leaching or runoff. Our study examined 1) the presence and concentration of eleven trace elements in a range of lab-created coal ash leachate solutions at neutral pH using ICP-OES, 2) the physiological effects of these leachate solutions on a freshwater gastropod (Planorbella duryi), and 3) the ability of these trace metals to bioaccumulate in the tissues of exposed individuals. As, Cd, Cu, Mg, Mn, and Pb were detected in solutions at increasing concentrations concurrent with ash concentration. Exposure to leachates caused significant delays in embryonic development, reduced juvenile shell growth, decreases in egg and clutch production, and the display of avoidance behaviors. Tissues of exposed snails contained elevated concentrations of As, Cd, Cu, and Cr, with bioconcentration factors 177,550 times higher in cadmium and 85,468 times higher in arsenic in the highest treatment compared to control organisms. Our results highlight the potential harmful effects of coal ash leachates on a novel freshwater invertebrate species using several unique methodologies, providing key information regarding their potential impacts on surrounding aquatic ecosystems.

China national food safety standards of cadmium in staple foods: Issues and thinking

<p indent="0mm">Cadmium (Cd), as a class one carcinogen, is easily transferred from soil to the food chain, representing a potential threat to human health. Rapid industrialization in China during the last three decades has caused widespread contamination in agricultural soils. As a result, a considerable proportion of the rice grain in some areas of southern China has concentrations of Cd exceeding the Chinese food safety standard. This problem has raised a widespread concern regarding food safety and brought a big debate in China about whether the current food safety standard is reasonable. The food safety standard of Cd is not only critical for assessing soil environmental quality, but also important to reduce the current elevated dietary Cd intake for the general population. In this perspective, we summarize the history of establishment and revision in Chinese food safety standards of Cd in staple foods and discuss whether the current Cd safety limits for both rice and wheat are reasonable in terms of the tolerable limit for dietary Cd intake, dietary pattern, the current exposure level of Cd for the general pollution, and social-economic and international trading factors. The dietary Cd intake for the general population has doubled over the last <sc>25 years,</sc> from <sc>13.8 μg/d</sc> in 1990 to <sc>32.7 μg/d</sc> in 2011–2015, with the latter being approximately 61% of the provisional tolerable intake recommended by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). The growing body of evidence suggests no safe Cd intake limit for human health and consequently, dietary intake of Cd should be reduced as much as possible. The milling (i.e., polishing) process has an uncertain effect on change in Cd concentration in polished rice compared to the brown rice because of the uncertainty of the Cd spatial distribution pattern in rice grain. Furthermore, polished rice has higher bioavailability of Cd to humans than brown rice. These differences should be considered when extrapolating the food safety standard between brown rice and polished rice. According to the current exposure level and risk of Cd through consumption of rice, we contend that the current food safety limit for rice grain <sc>(0.2 mg/kg)</sc> in China is lenient for both the southern and northern general populations. It is unadvisable to be looser in further revisions of Chinese food safety standards. For wheat grain, the current safety limit <sc>(0.1 mg/kg)</sc> is reasonable for the northern population but strict for the southern population. Given the current contamination status and national food security and safety, the principle of ALARA (as low as reasonably achievable) should be applied to guild the amendment of the national food safety standard of Cd. There are several scientific perspectives to be further studied. First, epidemiological studies are required to establish the tolerable intake limit suitable to the Chinese ethnic characteristics and dietary patterns. Second, the new food safety standard should consider the subgroup populations with high Cd exposure risk, including infants, children, and local farmers who mainly consume home-grown rice with elevated Cd concentrations in Cd-contaminated regions. To reduce Cd exposure to the local population, effective mitigation strategies include exchanging high Cd home-grown rice with low Cd rice and increasing dietary nutrients and human nutrition.

Abundance and characteristics of sediment-bound magnetic minerals and trace elements in karst ditch wetland: A case study from Guizhou Province, China

This study investigated sediment-bound magnetic properties and selected trace elements level in the karst ditch wetland, Caohai National Nature Reserve, Guizhou Province, China. Sediment-bound magnetic signals were quantified using low-frequency mass magnetic susceptibility (χLF), anhysteretic remanent magnetization susceptibility (χARM), saturation isothermal remanent magnetization (SIRM), and percentage frequency-dependent susceptibility (χfd%). Concentrations of Cd, Cr, Sb and Zn were determined using inductively coupled plasma mass spectrometry. Sediment χLF, χARM, SIRM, and χfd% were higher than those of bedrocks and mainly altered by the pedogenic processes. The estimated χfd% ranged from 6.15 % to 14.62 % and reflected the magnetic grain sizes were largely concentrated in the range of superparamagnetic particles. The elevated concentrations of sediment-bound Cd, Cr, Sb, and Zn supported the significant contribution of the anthropogenic sources in the karst ditch wetlands. The weak relationship between magnetic signals and selected trace elements (p < 0.05) suggested the occurrence of few sediment-bound iron-containing minerals associated with selected trace elements. These results indicated that a minor contribution of anthropogenic sources of selected trace elements to the elevated sediment magnetic signals in the karst ditch wetlands.

Intracellular sequestration of cadmium and zinc in ectomycorrhizal fungus Amanita muscaria (Agaricales, Amanitaceae) and characterization of its metallothionein gene

Amanita muscaria is an ectomycorrhizal mushroom that commonly grows at metal-polluted sites. Sporocarps from the lead smelter-polluted area near Příbram (Central Bohemia, Czech Republic) showed elevated concentrations of Cd and Zn. Size exclusion chromatography of the cell extracts of the sporocarps from both polluted and unpolluted sites indicated that substantial part of intracellular Cd and Zn was sequestered in 6-kDa complexes, presumably with metallothionein(s) (MT). When the cultured mycelial isolates were compared, those from Příbram were more Cd-tolerant and accumulated slightly less Cd and Zn than those from the unpolluted site. The analysis of the available A.muscaria sequence data returned a 67-amino acid (AA) MT encoded by the AmMT1 gene. Weak Cd and Zn responsiveness of AmMT1 in the mycelia suggested its metal homeostasis function in A.muscaria, rather than a major role in detoxification. The AmMT1 belongs to a ubiquitous peptide group in the Agaricomycetes consisting of 60–70-AA MTs containing seven cysteinyl domains and a conserved histidyl, features observed also in a newly predicted, atypical 45-AA RaMT1 of the Zn-accumulator Russula bresadolae in which the C-terminal cysteinyl domains VI and VII are missing. Heterologous expression in metal-sensitive yeast mutants indicated that AmMT1 and RaMT1 encode functional peptides that can protect cells against Cd, Zn, and Cu toxicity. The metal protection phenotype observed in yeasts with mutant variants of AmMT1 and RaMT1 further indicated that the conserved histidyl seems to play a structural, not metal binding role, and the cysteinyls of the C-terminal domains VI and VII are important for Cu binding. The data provide an important insight into the metal handling of site-associated ectomycorrhizal species disturbed by excess metals and the properties of MTs common in Agaricomycetes.

Passive Treatment of Circumneutral Mine Drainage from the St. Louis Mine Tunnel, Rico CO: Part 2—Vertical Biotreatment Train Pilot Study

AbstractThis is the second of three papers dealing with metal-bearing circumneutral mine drainage from the inactive Rico-Argentine mine site located at an elevation of ≈ 2740 m (9000 feet) in the San Juan mountain range in southwestern Colorado. This paper evaluates two years of mine drainage treatment using a passive system that included a vertical-flow engineered biotreatment cell. The collapsed St. Louis Tunnel (SLT) discharges circumneutral mine water from several sources that contains elevated concentrations of Cd, Cu, Fe, Mn, Zn. A demonstration-scale 114 L/min (30 gpm) gravity-flow passive treatment system was installed, consisting of a settling basin (utilizing coagulant addition to improve suspended solids settling efficiency), an anaerobic sulfate-reducing bioreactor, and an aeration cascade for effluent polishing. The treatment system generally met target treatment goals for Cd, Cu, Fe, and Pb. Nanophase ZnS in system effluent decreased the frequency of meeting total Zn project treatment goals. Unexpectedly high levels of Mn removal were observed in both the anaerobic bioreactor and the aeration cascade. Large seasonal variations in influent metals concentrations and pH present the greatest challenge in managing system performance.