Electrochemical Detection Of Glucose Research Articles

Controlling electrodeposited Ni layers by different-sized graphene oxides enables conductive e-textiles for the highly sensitive electrochemical detection of glucose

Controlling electrodeposited Ni layers by different-sized graphene oxides enables conductive e-textiles for the highly sensitive electrochemical detection of glucose

Highly Efficient Non-Enzymatic Electrochemical Glucose Biosensor Based on Copper Metal Organic Framework Coated on Graphite Sheet

Detection of glucose is highly informative, creating a constant demand for fabricating high-precision glucose biosensors. Metal–organic frameworks, a family of porous materials renowned for their tunability, can be an excellent choice for developing such sensors. We have developed a highly-sensitive, non-enzymatic sensor for electrochemical detection of glucose fabricated using Copper Metal–Organic Framework (Cu MOF), synthesized by a simple, room-temperature stirring method using Benzene-1,3,5-tricarboxylic acid (BTC) as ligand and Copper nitrate trihydrate as precursor. The synthesized nanostructure was characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray analytical techniques. Powder X-ray diffraction study and thermogravimetric analysis were also done. Further, Brunnauer-Emmett-Teller analysis revealed the porous nature of Cu MOF. The materials exhibited strong electro-catalytic activity for glucose oxidation as revealed from cyclic voltammetry and chronoamperometric studies done under alkaline pH conditions. The Cu MOF deposited on a conducting graphite sheet electrode displayed a significantly low detection limit of 0.019 mM through a broad detection range (1–15 mM) and a strong sensitivity of 229.4 μAmM−1 cm2. Overall, the Cu MOF/GS exhibits exceptional stability, short response time (less than 1 s), and good repeatability and reproducibility, making it a promising future material for non-enzymatic glucose detection.

Use of laser-induced graphene and magnetite nanoparticles as anchors in electrochemical glucose detection devices

Use of laser-induced graphene and magnetite nanoparticles as anchors in electrochemical glucose detection devices

Ag@TiO2 nanoribbon array: a high-performance sensor for electrochemical non-enzymatic glucose detection in beverage sample

Developing an accurate, cost-effective, reliable, and stable glucose detection sensor for the food industry poses a significant yet challenging endeavor. Herein, we present a silver nanoparticle-decorated titanium dioxide nanoribbon array on titanium plate (Ag@TiO2/TP) as an efficient electrode for non-enzymatic glucose detection in alkaline environments. Electrochemical evaluations of the Ag@TiO2/TP electrode reveal a broad linear response range (0.001 mM - 4 mM), high sensitivity (19,106 and 4264 μA mM−1 cm−2), rapid response time (6 s), and a notably low detection limit (0.18 μM, S/N = 3). Moreover, its efficacy in measuring glucose in beverage samples shows its practical applicability. The impressive performance and structural benefits of the Ag@TiO2/TP electrode highlight its potential in advancing electrochemical sensors for small molecule detection.

Tuning Co/Ni Ratio in Co–Ni Bimetallic Hybrid for Electrochemical Detection of Glucose

Tuning Co/Ni Ratio in Co–Ni Bimetallic Hybrid for Electrochemical Detection of Glucose

3D gyroscopic copper-based metal-organic framework for high sensitivity detection of glucose and H2O2

In this work, gyroscopic Cu-BDC-NH2 are obtained by a simple one-step solvothermal method, which can be used for non-enzymatic electrochemical detection of hydrogen peroxide (H2O2) and glucose. The gyroscopic morphology, abundant active sites and excellent biocompatibility of Cu-BDC-NH2 improve its excellent electrocatalytic property for detecting glucose and H2O2. The prepared Cu-BDC-NH2 modified electrode nanomaterials can realize the ultra-sensitive detection of glucose and H2O2 with sensitivity of 6121 and 1212 μA mM−1 cm−2, and detection limits of 1.2 and 1.5 µM, respectively. Meanwhile, Cu-BDC-NH2-modified electrodes exhibit excellent reproducibility, anti-interference and stability. Additionally, the sensor is effectively used to determine glucose and H2O2 in human serums. It suggests that it has a promising future for detecting glucose and hydrogen peroxide in real-world samples.

Designing of high performance MoS2@VZnS//AC hybrid battery supercapacitor device for the electrochemical energy storage and glucose detection

Due to their high conductive properties and superior specific capacitance, metal sulfides exhibit remarkable electrical conductivity. Transition metal dichalcogenides (TMDs) are regarded ideal options for energy harvesting devices due to their unique 2D arrangement and remarkable electrochemical properties. In this work, the typical exfoliation method (tip sonication method) was used to synthesize the molybdenum disulfide (MoS2). By using the hydrothermal method, the vanadium zinc sulfide is synthesized. The composite was analyzed through SEM, XRD, and XPS techniques to investigate its morphological, structural, and compositional characteristics comprehensively. The MoS2@VZnS electrode achieved a specific capacity (Qs) of 1025 Cg−1 at 3 mVs−1 after being tested in 1 M KOH. In a two-electrode set-up, activated carbon is used as a negative electrode, and MoS2@VZnS is used as a positive electrode for the fabrication of a hybrid supercapacitor. The asymmetric device has demonstrated a Qs of 285.4 Cg−1 at 1.4 Ag−1. The fabricated asymmetric supercapacitor device has manifested an excellent energy density (Ed) of 39.5 Whkg−1 and power density (Pd) of 2462.35 Wkg−1. Furthermore, in the durability test of MoS2@VZnS//AC, it exhibited an impressive capacity retention of 91.32% after enduring 5,000 cycles. Besides this, an investigation was conducted on a glucose electrochemical sensor utilizing MoS2@VZnS, to enhance its performance. The device showed extraordinary performance and considerable sensitivity against glucose, even up to a very high value. The multifunctional MoS2@VZnS nanocomposite electrode material provides new opportunities to design hybrid devices in energy storage devices and biomedical applications.

Copper-cobalt dual-site on N-doped carbon nanotube with dual-promoted synergy for glucose electrochemical detection

Doping specific active sites and accelerating the decisive step of glucose catalysis to construct highly active glucose sensing electrochemical catalysts remains a major challenge for glucose sensing. Herein, we report the detailed design of Cu–Co dual active site N-doped carbon nanotube (CuCo-NCNTs) obtained by electrodeposition modification, programmed warming and calcination for electrochemical glucose detection. In the CuCo-NCNTs material system, Cu serves as the main active site for glucose sensing. Co with good adsorption of hydroxyl groups acts as the site providing hydroxyl groups to provide oxygen source for Cu oxidized glucose sensing. The synergistic effect between the two active sites in the Cu–Co system and the abundant micro-reactive sites exposed by carbon nanotubes greatly ensure the excellent electrocatalytic performance of glucose oxidation reaction. Therefore, CuCo-NCNTs have good electrocatalytic performance with a sensitivity of 0.84 mA mM−1 cm−2 and a detection limit of 1 μM, and also have excellent stability and specificity. DFT calculations elucidate the decisive steps of H-atom removal in the oxidation of glucose by Cu active site N-doped carbon nanotube (Cu-NCNTs) and Co active site N-doped carbon nanotube (CuCo-NCNTs) materials, illustrating the role of oxygen source provided by hydroxyl group adsorption in the electrochemical sensing process of glucose, thus demonstrating that the electrochemical sensing signal of glucose can be effectively enhanced when cobalt species that readily adsorb hydroxyl groups are introduced into the materials.

Enhancing self-powered wearable device performance: ZIF-8/rGO hybrid nanostructures for extended operation and electrochemical glucose detection

The growing demand for wearable devices designed for continuous monitoring necessitates an extended power supply, prompting the development of self-powered sensing devices capable of prolonged operation. Multifunctional materials have become a prime focus for self-powered systems. One such material is ZIF-8, a porous crystalline metal–organic framework (MOF) that holds promise for non-enzymatic glucose detection due to its expansive surface area, functional sites, electrocatalytic activity, and biocompatibility. Our research details the successful synthesis of ZIF-8 and a composite consisting of ZIF-8 and reduced graphene oxide (rGO). We extensively investigated the structural, elemental, and morphological attributes of these materials using various characterization techniques. Our examination of glucose detection involved cyclic voltammetry and amperometry studies. The hybrid electrode, benefiting from the synergistic interplay between ZIF-8 and rGO, exhibited an exceptional sensitivity of 5047.18 μA mM−1 cm−2 and a detection limit of 0.3 μM, spanning the range of 0.005 mM to 5 mM. Furthermore, we explored these materials' potential in the supercapacitance realm. The ZIF-8/rGO hybrid electrode demonstrated an impressive specific capacitance of 287F/g at a current density of 1 Ag−1, displaying pseudocapacitive behaviour. A proof-of-concept prototype device, utilizing ZIF-8/rGO, was fabricated and investigated for its potential in wearable glucose sensing. The device yielded satisfactory results, showcasing its capability as a self-powered wearable sensor. In general, ZIF-8/rGO hybrid nanostructure emerged as a promising candidate for supercapacitor and electrochemical glucose detection applications.

Electrochemically Deposited Cu(II)/Cu(I) Oxide Heterostructure as Highly Sensitive Platform for Electrochemical Detection of Glucose and Methanol

Electrochemically Deposited Cu(II)/Cu(I) Oxide Heterostructure as Highly Sensitive Platform for Electrochemical Detection of Glucose and Methanol

Low-interference and sensitive electrochemical detection of glucose and lactate using boron-doped diamond electrode and electron mediator menadione.

To minimize background interference in electrochemical enzymatic biosensors employing electron mediators, it is essential for the electrochemical oxidation of electroactive interfering species (ISs), such as ascorbic acid (AA), to proceed slowly, and for the redox reactions between electron mediators and ISs to occur at a low rate. In this study, we introduce a novel combination of a working electrode and an electron mediator that effectively mitigates interference effects. Compared to commonly used electrodes such as Au, glassy carbon, and indium tin oxide (ITO), boron-doped diamond (BDD) electrodes demonstrate significantly lower anodic current (i.e., lower background levels) in the presence of AA. Additionally, menadione (MD) exhibits notably slower reactivity with AA compared to other electron mediators such as Ru(NH3)63+, 4-amino-1-naphthol, and 1,4-naphthoquinone, primarily due to the lower formal potential of MD compared to AA. This synergistic combination of BDD electrode and MD is effectively applied in three biosensors: (i) glucose detection using electrochemical-enzymatic (EN) redox cycling, (ii) glucose detection using electrochemical-enzymatic-enzymatic (ENN) redox cycling, and (iii) lactate detection using ENN redox cycling. Our developed approach significantly outperforms the combination of ITO electrode and MD in minimizing IS interference. Glucose in artificial serum can be detected with detection limits of ~ 20μM and ~ 3μM in EN and ENN redox cycling, respectively. Furthermore, lactate in human serum can be detected with a detection limit of ~ 30μM. This study demonstrates sensitive glucose and lactate detection with minimal interference, eliminating the need for (bio)chemical agents to remove interfering species.

Copper-based bimetallic metal–organic framework in-situ embedded of heterogeneous Cu nanoparticles for enhanced nonenzymatic glucose sensing

Copper-based metal–organic frameworks (MOFs) are recently emerging as encouraging electrocatalysts with high performance and stability for electrochemical detection of glucose. Herein, a simple one-step solvothermal in-situ derivative strategy is proposed to fabricate the novel heterogeneous electrocatalyst of Cu nanoparticles embedded bimetallic Cu@MCu-BDC-NH2 (M = Ni, Co and Zn) MOFs, which can enhance the conductivity via tailor the electronic structure and heterojunction interface. The optimal bimetallic Cu@CoCu-BDC-NH2 heterostructure exposes more active sites, the electronic interaction between metal-O enhanced the reactivity and electrical conductivity, and prominently boost the kinetics of glucose detection. The optimized Cu@CoCu-BDC-NH2 heterojunction structure as modified electrode nanomaterials exhibit the low detection limit (0.27 μM), wide linear range (0.5 μM–2 mM) and the high sensitivity (21157 μA mM−1 cm−2), which are successfully used in human serum and hold great stability and anti-interference. The density functional theory (DFT) calculation demonstrates that the adsorption energies of Cu@CoCu-BDC-NH2 heterostructure for glucose are −0.3 eV, suggesting good adsorption capacity for glucose. This work not only successfully establish Cu nanoparticles heterostructure interface with bimetallic MOFs for high-performance glucose sensors, but also emphasizes the importance of regulate the electronic microstructure of bimetallic MOFs for enhanced reaction kinetics.

Development of Redox-Active Lyotropic Lipid Cubic Phases for Biosensing Platforms.

Enzyme-based electrochemical biosensors play an important role in point-of-care diagnostics for personalized medicine. For such devices, lipid cubic phases (LCP) represent an attractive method to immobilize enzymes onto conductive surfaces with no need for chemical linking. However, research has been held back by the lack of effective strategies to stably co-immobilize enzymes with a redox shuttle that enhances the electrical connection between the enzyme redox center and the electrode. In this study, we show that a monoolein (MO) LCP system doped with an amphiphilic redox mediator (ferrocenylmethyl)dodecyldimethylammonium bromide (Fc12) can be used for enzyme immobilization to generate an effective biosensing platform. Small-angle X-ray scattering (SAXS) showed that MO LCP can incorporate Fc12 while maintaining the Pn3m symmetry morphology. Cyclic voltammograms of Fc12/MO showed quasi-reversible behavior, which implied that Fc12 was able to freely diffuse in the lipid membrane of LCP with a diffusion coefficient of 1.9 ± 0.2 × 10-8 cm2 s-1 at room temperature. Glucose oxidase (GOx) was then chosen as a model enzyme and incorporated into 0.2%Fc12/MO to evaluate the activity of the platform. GOx hosted in 0.2%Fc12/MO followed Michaelis-Menten kinetics toward glucose with a KM and Imax of 8.9 ± 0.5 mM and 1.4 ± 0.2 μA, respectively, and a linearity range of 2-17 mM glucose. Our results therefore demonstrate that GOx immobilized onto 0.2% Fc12/MO is a suitable platform for the electrochemical detection of glucose.

Rapid and facile preparation of NiFe-layered double hydroxide nanosheets as self-supported electrode for glucose detection in drink sample

Layered double hydroxides have been widely used for electrochemical glucose detection due to their layered structure with more active sites, but they suffer from lower electrical conductivity and long-time hydrothermal preparation. In this paper, NiFe-layered double hydroxide nanosheets supported on nickel foam (NiFe-LDH NSs/NF) was prepared using an ultrafast and facile method via in-situ corroding foam nickel in FeCl3 solution under room temperature, and the whole synthetic process can be accomplished within several minutes. The as-fabricated NiFe-LDH NSs/NF shows significant catalytic activity in the glucose oxidation, showing its great promise in glucose detection. As a self-supported electrode, NiFe-LDH NSs/NF is favorable for glucose detection, with a sensitivity of 9.79 and 3.29 mA mM−1 cm−2 within the linear range of 0.001 to 1.16 mM and 1.16 to 4.67 mM, respectively. Moreover, NiFe-LDH NSs/NF is also selective and reliable towards glucose detection in drink sample.

A Multifunctional Biosensor via MXene Assisted by Conductive Metal–Organic Framework for Healthcare Monitoring

AbstractFor real‐time care of metabolic diseases, it is highly demanded to simultaneously monitor metabolite content and diagnose muscle strength in a rapid and convenient way. However, such a biosensor with integrated functions of detecting chemical and biopotential signals at the same time has seldom been reported. In this work, a multifunctional biosensor, integrating the electrochemical detection of uric acid (UA) and glucose (Glu) in sweat, electrophysiological signal acquisition, and electrostimulation therapy, is designed. Hence, an electrophysiological MOF/MXene electrode is fabricated using highly conductive MXene and porous conductive metal–organic framework (c‐MOF, Ni3(HITP)2). Compared with commercial Ag/AgCl electrode, MOF/MXene electrode has lower electrode/skin interface impedance, higher stability and signal‐to‐noise (SNR). By utilizing the high charge storage capacity (CSC) of MOF/MXene electrode, it can realize muscle treatment by electrostimulation. Besides, with abundant active regions of c‐MOF, the electrode exhibits excellent electrochemical performance in detecting sweat metabolites. Multimodal signals detected by such integrated biosensors are wirelessly transmitted to a mobile terminal, showing significant potential on muscle theranostics and daily non‐invasive monitoring. Additionally, they offer timely nutritional guidance for individuals dealing with hyperuricemia or hyperglycemia.

Zwitterionic hydrogel for preserving stability and activity of oxidase enzyme for electrochemical biosensor

Enzymatic electrochemical biosensor is the most common analytical platform for medical diagnosis. To mimic the biological environment of the enzyme for maintaining the function of biosensor, zwitterionic hydrogels have been recognized as effective matrices for enzymatic immobilization. Herein, a zwitterionic hydrogel derived from a copolymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-N-methacryloyloxyethyl tyrosine methylester (MAT)] (PMM) was firstly applied as versatile coating to preserve stability and activity of oxidase enzymes, glucose oxidase (GOx) and lactate oxidase (LOx) for enzymatic electrochemical sensor. A screen-printed carbon electrode (SPCE) was sequentially coated with nitrogen-doped graphene (NDG), oxidase enzyme, and PMM mixed with Ru(II)bpy32+ and (NH4)2S2O8 followed by visible light irradiation for 3 min to induce PMM gelation. Electrochemical detection of glucose and lactate using the modified SPCE was performed via amperometry in the presence of hydrogen peroxide. The activity of both GOx and LOx immobilized on the modified SPCE was well maintained for 49 days at 87 and 80 %, respectively. Additionally, two different electrodes, a screen-printed graphene electrode (SPGE), and a screen-printed silver electrode (SPAgE), similarly modified gave the same satisfactory detection of spiked glucose and lactate in human plasma and sweat with 93–118 % recovery. This indicates the potential of the PMM hydrogel as a universal platform for preservation of enzymes which can be easily fabricated without the need for specific chemical modification of the electrode.

Nanoporous CuZn supported CuO nanoparticle electrodes for non-enzymatic electrochemical glucose detection

Nanoporous CuZn supported CuO nanoparticle electrodes for non-enzymatic electrochemical glucose detection

A Wearable Touch-Activated Device Integrated with Hollow Microneedles for Continuous Sampling and Sensing of Dermal Interstitial Fluid.

Dermal interstitial fluid (ISF) is emerging as a rich source of biomarkers that complements conventional biofluids such as blood and urine. However, the impact of ISF sampling in clinical applications has been limited owing to the challenges associated with extraction. The implementation of microneedle-based wearable devices that can extract dermal ISF in a pain-free and easy-to-use manner has attracted growing attention in recent years. Here, a fully integrated touch-activated wearable device based on a laser-drilled hollow microneedle (HMN) patch for continuous sampling and sensing of dermal ISF is introduced. The developed platform can produce and maintain the required vacuum pressure (as low as ≈ -53kPa) to collect adequate volumes of ISF (≈2µL needle-1 h-1 ) for medical applications. The vacuum system can be activated through a one-touch finger operation. A parametric study is performed to investigate the effect of microneedle array size, vacuum pressure, and extraction duration on collected ISF. The capability of the proposed platform for continuous health monitoring is further demonstrated by the electrochemical detection of glucose and pH levels of ISF in animal models. This HMN-based system provides an alternative tool to the existing invasive techniques for ISF collection and sensing for medical diagnosis and treatment.

Green synthesis of copper oxide nanoparticles using Amaranthus dubius leaf extract for sensor and photocatalytic applications

Copper oxide nanoparticles (CuO NPs) were produced through an environmentally friendly green synthesis. The characteristics of these green synthesized CuO NPs, including their structural, optical, morphological, and electrochemical properties, were examined using various characterization techniques. X-ray diffraction analysis revealed that the CuO NPs have a monoclinic structure with a C2/c space group. Electrochemical detection of glucose was carried out using cyclic voltammetry. The green synthesized CuO NPs exhibited excellent catalytic properties for both electrochemical sensing and photocatalysis. Significantly, these CuO NPs exhibited excellent selectivity and sensitivity in glucose detection, with a sensitivity of 370 μA mM−1 cm−2 and a detection limit of 1.0 μM. Furthermore, the CuO NPs demonstrated a substantial 84 % degradation of dyes within 150 min. These results underscore the potential of the green synthesized CuO NPs as a promising material for applications in both sensing and dye degradation.

Immobilizing glucose oxidase on AuCu hydrogels for enhanced electrochromic biosensing

Development of highly sensitive and accurate biosensors still faces a great challenge. Herein, glucose oxidase (GOx) is efficiently immobilized on the AuCu hydrogels owing to their porous structure and interfacial interaction, demonstrating enhanced catalytic activity, satisfactory stability and recyclability. Besides, by integration of AuCu@GOx and electrochromic material of Prussian blue, a sensitive and stable biosensing platform based on the excellent electrochromic property of Prussian blue and the enhanced enzyme activity of AuCu@GOx is developed, which enables the electrochemical and visual dual-mode detection of glucose. The as-constructed biosensing platform possesses a wide linear range, and good selectivity for glucose detection with a limit of detection of 0.82 μM in visual mode and 0.84 μM in electrochemical mode. This easy-to-operate biosensing platform opens a door for the practical application of the multi-mode strategy for glucose detection.