Elastic Modulus Of Gels Research Articles

Natural Polyelectrolyte Hydrogels with Two Types of Cross-Links with Different Energy

For the first time, gels of a polymer cross-linked by two types of ionic cross-links of different energies, namely trivalent chromium (III) ions and divalent iron (II) ions, were prepared. The negatively charged polysaccharide xanthan, a polyelectrolyte of natural origin, was used as a polymer. A study of xanthan gels cross-linked with each cross-linker separately was carried out to identify the optimal concentrations of each cross-linker for the production of a double cross-linked gel. The mechanical properties of hydrogels under oscillatory shear deformations were then studied. It was demonstrated that the simultaneous use of two cross-linkers resulted in a synergistic increase in the elastic modulus (plateau storage modulus) compared to gels cross-linked with each cross-linker separately. For a gel with two types of cross-links, the elastic modulus was 16 Pa. In contrast, for gels cross-linked with either chromium (III) cations or iron (II) cations, the elastic modulus was approximately 0.6 and 0.5 Pa, respectively. The strong effect can be attributed to the different nature of cross-linking between xanthan macromolecules and chromium (III) and iron (II) cations, as well as the different strength of the cross-links formed. The optimal range of pH values was determined in which a synergistic increase in the elastic modulus of gels with two types of cross-links was observed compared to the corresponding gels cross-linked with each cross-linker separately. Consequently, the concurrent utilisation of two cross-linking agents that generate cross-links with disparate energy levels represents an efficacious strategy to improve the tensile strength of polymer hydrogels.

Crack-filling effect of gel on time-dependent mechanical behavior of concrete damaged by alkali–silica reaction

This study aims to investigate the crack-filling effect of gel generated by the alkali–silica reaction (ASR) on the mechanical behavior of concrete and propose an analytical model to predict the compression behavior of ASR-damaged concrete. To identify the mechanical contribution of the gel-filling cracks, the distribution of the chemical compositions and elastic moduli of the crack-filling gels were observed using SEM–EDS analysis and nano-indentation tests. The experimental results showed that the elastic modulus of the gel with a high-calcium composition ranged from 30 to 40 GPa, which is comparable to the elastic modulus of concrete. In addition, through literature reviews of the time-dependent compression behaviors of ASR-affected concrete, it was hypothesized that crack-filling gels can contribute to the recovery of ASR damage, and that the mechanical contribution of gels depends on the ASR crack patterns and changes with time. Based on these assumptions, the resistance of the gels filling the cracks in the compression, shear, and recontact behaviors of ASR-damaged concrete was reflected in the proposed model, in which the gel resistance increased with time and became larger as the ratio of microcracks to total cracks increased. The proposed model was verified through a comprehensive comparison of analysis and test results of compression behavior of ASR-damaged specimens, and it appeared that the change in mechanical properties of concrete according to the degree of damage (ASR expansion) and time was well simulated by the proposed model.

Tribological and Rheological Study of Thixotropic Gels of 2D Nanoparticles.

A thixotropic colloidal gel constituting an aqueous dispersion of synthetic clay Laponite with varying concentrations of salt has been studied for its rheological and tribological performance as a lubricant. We observed that the incorporation of NaCl induces notable enhancements in the colloidal gel's relaxation time, elastic modulus, and yield stress. Although an increase in NaCl concentration decreases the material's relaxation time dependence on waiting time (tw), overall, the strength of its thixotropic character has been observed to increase with an increase in salt concentration. The analysis of friction and wear indicated that the utilization of a thixotropic colloidal gel of Laponite with a higher concentration of NaCl resulted in progressively greater reductions in both the coefficient of friction and specific wear rates under various load-speed conditions. Severe abrasive wear on disc surface under dry test, gradually mitigated upon the introduction of these lubricants. Two simultaneous lubricating mechanisms, first, the smooth sliding of the friction pair, facilitated by the alignment of Laponite particles in the direction of shear forces, and second, the stable structure of Laponite, coupled with the addition of NaCl, enabling continuous replenishment of the wear track with lubricant, are attributed to lubrication effectiveness.

Inline Determination of the Gel Elastic Modulus During Milk Coagulation Using a Multifiber Optical Probe

AbstractFrom a state-of-the-art point of view, it is currently possible to optically monitor the enzymatic coagulation of milk for real time estimation of the elastic modulus to cut the gel at optimum gel firmness. However, European cheese industry produces a wide variety of cheeses, many of them artisanal, and has a very fragmented productive structure with many small-, medium-sized companies. Therefore, if the technology is to be successfully uptake, it must be not only accurate but very low-cost. The objective of this work was to evaluate a low-cost commercial multifiber probe, for inline optical determination of curd firmness during cheese making. Preliminary tests were carried out to select the most appropriate fiber core size and wavelength and after that coagulation trials were performed following a fully randomized factorial design with two factors, i.e., concentration of protein (3.2, 3.6 and 4.0%) and added calcium (150, 200 and 250 mg L−1), with three replicates. The observed linear increase of the least square means of the initial voltage with the protein content (V0 = 0.15[P, %] + 0.88; R2 = 0.999), will be likely synergistic with the elastic modulus prediction if the model needs to be corrected for protein. Finally, the multifiber probe allowed predicting curd firmness using the proposed model with SEP values < 7 Pa. The present work has proven that a low-cost multifiber probe is suitable for accurate, real-time prediction of curd firmness during cheese manufacture.

Spatial and Temporal Control of 3D Hydrogel Viscoelasticity through Phototuning.

The mechanical properties of the extracellular environment can regulate a variety of cellular functions, such as spreading, migration, proliferation, and even differentiation and phenotypic determination. Much effort has been directed at understanding the effects of the extracellular matrix (ECM) elastic modulus and, more recently, stress relaxation on cellular processes. In physiological contexts such as development, wound healing, and fibrotic disease progression, ECM mechanical properties change substantially over time or space. Dynamically tunable hydrogel platforms have been developed to spatiotemporally modulate a gel's elastic modulus. However, dynamically altering the stress relaxation rate of a hydrogel remains a challenge. Here, we present a strategy to tune hydrogel stress relaxation rates in time or space using a light-triggered tethering of poly(ethylene glycol) to alginate. We show that the stress relaxation rate can be tuned without altering the elastic modulus of the hydrogel. We found that cells are capable of sensing and responding to dynamic stress relaxation rate changes, both morphologically and through differences in proliferation rates. We also exploited the light-based technique to generate spatial patterns of stress relaxation rates in 3D hydrogels. We anticipate that user-directed control of the 3D hydrogel stress relaxation rate will be a powerful tool that enables studies that mimic dynamic ECM contexts or as a means to guide cell fate in space and time for tissue engineering applications.

Coagulation and proteolysis of high-protein milks in the gastric environment

Gastric digestion of 2 commercial ultrafiltered milks and milk enriched with skim milk powder (to simulate concentration by reverse osmosis) was investigated and compared with the digestion of nonconcentrated milk. Curd formation and proteolysis of high-protein milks in simulated gastric conditions were studied using oscillatory rheology, extrusion testing, and gel electrophoresis. The presence of pepsin in the gastric fluid triggered coagulation at pH >6 and the elastic modulus of gels from high-protein milks was ~5 times larger than the gel from reference milk. Despite similar protein concentrations, the coagulum from milk enriched with skim milk powder showed higher resistance to shear deformation than the coagula from ultrafiltered milks. The gel structure was also more heterogeneous. During digestion, the degradation of coagula from high-protein milks was slowed down compared with the coagulum from reference milk, and intact milk proteins were still detected after 120 min. Differences in the digestion patterns of coagula from high-protein milks were observed and were associated with the proportion of minerals bound to caseins and the denaturation rate of whey proteins.

Ionogel Electrolytes Supported by Zwitterionic Copolymers Featuring Lithium Ion-Mediated, Noncovalent Cross-Links

A series of ionogel electrolytes, composed of an ionic liquid/lithium salt solution supported by a linear zwitterionic (ZI) copolymer scaffold containing solely noncovalent cross-links, were created in order to study the effects of varying the ZI fraction within the copolymer as well as the lithium ion concentration on gel mechanical properties and ion transport. The copolymer scaffold was synthesized via in situ UV photopolymerization of the ZI monomer 2-methacryloyloxyethyl phosphorylcholine (MPC) together with the non-ZI comonomer 2,2,2-trifluoroethyl methacrylate (TFEMA) in varying ratios, using a fixed fraction of 25 mol % total monomers. Ionic liquid electrolyte (ILE) solutions of 0.1, 0.3, 0.5, or 1 M lithium bis(trifluoromethylsulfonyl)imide (LiTFSI) dissolved in the ionic liquid 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide (BMP TFSI) were employed. For ionogels created using the 1 M LiTFSI ILE, varying the MPC content from 6.3 mol % to 17 mol % in the precursor solution resulted in a substantial increase in gel elastic modulus, from 20 kPa to 11 MPa. In contrast, room temperature ionic conductivity values for the same samples remained nearly constant, varying from 0.57–0.70 mS cm–1. While the in situ photopolymerization approach remains highly versatile for ionogel formation, an alternative method is introduced here for the first time that can produce ionogels “on-demand” using a pre-made ZI copolymer/ionic liquid solution to which a Li+ ILE is subsequently added.

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton.

Cells can actively change their shapes and become motile, a property that depends on their ability to actively reorganize their internal structure. This feature is attributed to the mechanical and dynamic properties of the cell cytoskeleton, notably, the actomyosin cytoskeleton, which is an active gel of polar actin filaments, myosin motors, and accessory proteins that exhibit intrinsic contraction properties. The usually accepted view is that the cytoskeleton behaves as a viscoelastic material. However, this model cannot always explain the experimental results, which are more consistent with a picture describing the cytoskeleton as a poroelastic active material-an elastic network embedded with cytosol. Contractility gradients generated by the myosin motors drive the flow of the cytosol across the gel pores, which infers that the mechanics of the cytoskeleton and the cytosol are tightly coupled. One main feature of poroelasticity is the diffusive relaxation of stresses in the network, characterized by an effective diffusion constant that depends on the gel elastic modulus, porosity, and cytosol (solvent) viscosity. As cells have many ways to regulate their structure and material properties, our current understanding of how cytoskeleton mechanics and cytosol flow dynamics are coupled remains poorly understood. Here, an in vitro reconstitution approach is employed to characterize the material properties of poroelastic actomyosin gels as a model system for the cell cytoskeleton. Gel contraction is driven by myosin motor contractility, which leads to the emergence of a flow of the penetrating solvent. The paper describes how to prepare these gels and run experiments. We also discuss how to measure and analyze the solvent flow and gel contraction both at the local and global scales. The various scaling relations used for data quantification are given. Finally, the experimental challenges and common pitfalls are discussed, including their relevance to cell cytoskeleton mechanics.

Exploring relationships between seaweeds carrageenan contents and extracted hybrid carrageenan properties in wild and cultivated Mastocarpus stellatus, Chondrus crispus and Ahnfeltiopsis devoniensis.

The types and amounts of carrageenans present in a set of Mastocarpus stellatus, Chondrus crispus and Ahnfeltiopsis devoniensis, are assessed by solid-state NMR performed directly on dried and powdered seaweeds. The resulting chemical analysis is compared with the chemical structure and gel elasticity of the hybrid carrageenans extracted from these three carrageenophytes. ν-carrageenan is only found in a single sample of M. stellatus, whereas C. crispus and A. devoniensis essentially contain ι- and μ-carrageenans. A significant amount of κ-carrageenan is found in all samples of M. stellatus, whereas this type of carrageenan was found in a single sample of A. devoniensis. Hybrid carrageenans (HK) made of κ-, ι-, μ- and ν-carrageenan units were found in all hot extracts from the seaweeds, and their respective contents are in line with earlier reports on the HK extracted from these seaweeds. No correlations between the carrageenan contents in eight seaweeds and the chemical structures of their corresponding extracted HK can be found. However, a positive nonlinear correlation between the intrinsic viscosity of extracted HK and their gel elastic modulus is found in a set of 8 carrageenophytes.

Eco-Friendly Synthesized PVA/Chitosan/Oxalic Acid Nanocomposite Hydrogels Embedding Silver Nanoparticles as Antibacterial Materials.

PVA/chitosan (PVA/CS) composite hydrogels incorporating silver nanoparticles (AgNPs) were prepared by double-cross-linked procedures: freeze–thawing and electrostatic interactions. Oxalic acid (OA) was used both for solubilization and ionic cross-linking of CS. AgNPs covered by CS (CS-AgNPs) with an average diameter of 9 nm and 18% silver were obtained in the presence of CS, acting as reducing agent and particle stabilizer. The increase of the number of freeze–thaw cycles, as well as of the PVA:CS and OA:CS ratios, resulted in an increase of the gel fraction and elastic modulus. Practically, the elastic modulus of the hydrogels increased from 3.5 kPa in the absence of OA to 11.6 kPa at a 1:1 OA:CS weight ratio, proving that OA was involved in physical cross-linking. The physicochemical properties were not altered by the addition of CS-AgNPs in low concentration; however, concentrations higher than 3% resulted in low gel fraction and elastic modulus. The amount of silver released from the composite hydrogels is very low (<0.4%), showing that AgNPs were well trapped within the polymeric matrix. The composite hydrogels displayed antimicrobial activity against S. aureus, K. pneumoniae or P. gingivalis. The low cytotoxicity and the antibacterial efficacy of hydrogels recommend them for wound and periodontitis treatment.

Synthesis and Characterization of BaSO4-CaCO3-Alginate Nanocomposite Materials as Contrast Agents for Fine Vascular Imaging.

Microcomputed tomography is an important technique for distinguishing the vascular network from tissues with similar X-ray attenuation. Here, we describe a composite of barium sulfate (BaSO4) nanoparticles, calcium carbonate (CaCO3) nanoparticles, and alginate that provides improved performance over microscale BaSO4 particles, which are currently used clinically as X-ray contrast agents. BaSO4 and CaCO3 nanoparticles were synthesized using a polyol method with tetraethylene glycol as solvent and capping agent. The nanoparticles show good colloidal stability in aqueous solutions. A deliverable nanocomposite gel contrast agent was produced by encapsulation of the BaSO4 and CaCO3 nanoparticles in an alginate gel matrix. The gelation time was controlled by addition of d-(+)-gluconic acid δ-lactone, which controls the rate of dissolution of the CaCO3 nanoparticles that produce Ca2+ which cross-links the gel. Rapid cross-linking of the gel by Ba2+ was minimized by producing BaSO4 nanoparticles with an excess of surface sulfate. The resulting BaSO4-CaCO3 nanoparticle alginate gel mechanical properties were characterized, including the gel storage modulus, peak stress and elastic modulus, and radiodensity. The resulting nanocomposite has good viscosity control and good final gel stiffness. The nanocomposite has gelation times between 30 and 35 min, adequate for full body perfusion. This is the first nanoscale composite of a radiopaque metal salt to be developed in combination with an alginate hydrogel and designed for medical perfusion and vascular imaging applications.

Preparation of konjac glucomannan/xanthan gum/sodium alginate composite gel by freezing combining moisture regulation

Specific foods such as connective tissue analogues are increasingly popular for the growing market demands. Hydrogels are considered to possess the potential to simulate connective tissue, however the relatively low mechanical strength of the conventional hydrogel greatly limited its application prospects. In the present study, we aimed to explore the effects of different freezing time, freezing-thawing (FT) cycles, and moisture content on the mechanical properties of konjac glucomannan/xanthan gum/sodium alginate (KGM/XG/SA) composite gel. After getting the freezing treatment, a noticeable change could be observed in the texture properties of the composite gel. As the FT cycles increased, the gel's network became denser, with its pores getting smaller. According to mechanical testing results, it is advisable to freeze the gel for 46 h at −20 °C, while the mechanical strength of the FT-2 is the best among samples. In addition, as the moisture content decreased from 92% to 60%, gel's elastic modulus, tensile strength and toughness increased significantly, and their maximum values were 27.1-, 12.9- and 10.7-fold of the non-dehydrated samples, respectively. Their porous structure would disappear gradually after undergoing the dehydration treatment, resulting in an extremely dense gel network with a significant increase in its mechanical strength. The present study suggests that both the freezing treatment and moisture regulation could effectively change the texture properties of the KGM/XG/SA composite gel, which provides a facile and efficient strategy for designing hydrogels products with exceptional texture and sensory characteristics.

Guar Gel Binders for Silicon Nanoparticle Anodes: Relating Binder Rheology to Electrode Performance.

Binding agents are a critical component of Si-based anodes for lithium-ion batteries. Herein, we introduce a composite hydrogel binder consisting of carbon black (CB) and guar, which is chemically cross-linked with glutaraldehyde as a means to reinforce the electrode structure during lithiation and improve electronic conductivity. Dynamic rheological measurements are used to monitor the cross-linking reaction and show that rheology plays a significant role in binder performance. The cross-linking reaction occurs at a faster rate and produces stronger networks in the presence of CB, as evidenced from higher gel elastic modulus in guar + CB gels than guar gels alone. Silicon nanoparticle (SiNP) electrodes that use binders with low cross-link densities (trxn < 2 days) demonstrate discharge capacities ∼1200 mAh g-1 and Coulombic efficiencies >99.8% after 300 cycles at 1-C rate. Low cross-link densities likely increase the capacity of SiNP anodes because of binder-Si hydrogen-bonding interactions that accommodate volume expansions. In addition, the cross-linked binder demonstrates the potential for self-healing, as evidenced by an increased elastic modulus after the gel was mechanically fragmented, which may preserve the electrode microstructure during lithiation and increase capacity retention. The composite hydrogel with integrated conductive additives gives promise to a new type of binder for next-generation lithium-ion batteries.

Effects of the structure and gel properties of myofibrillar protein on chicken breast quality treated with ultrasound-assisted potassium alginate

This paper aimed to evaluate the effects of the structure and gel properties of myofibrillar protein (MP) on 300-days-old chicken breast quality treated with ultrasound (300 W) and low addition (4 mg/mL) of potassium alginate (UPA). The results showed that UPA group exhibited lower liquid loss and optimized texture correlated with the formed water barrier and damaged myofibrils. UPA reduced the MP size and increased its solubility, and the decreased myosin thermostability and dissociated actomyosin reduced heating time for improved texture. UPA improved the gel strength, elastic modulus and ordered-arrangement of network. During gelation, the aggregation of myosin head was weakened and cross-linking of myosin tail and PA molecules was enhanced by hydrophobic interactions. UPA further inhibited the formation of disulfide bonds of myosin head and increased gel firmness. The lower myosin gelling temperature thus accelerated gel formation, and enhanced protein association with PA molecules facilitated the better gel performance.

Gelation of whey protein fractal aggregates induced by the interplay between added HCl, CaCl2 and NaCl

Gelation of whey protein fractal aggregates was induced by simultaneously adding salt (CaCl2 or NaCl) and acid (HCl). Further, simultaneous addition of both specifically binding (Ca2+) and non-binding (Na+) cations was studied at a fixed protein charge density. The effect of adding mixtures of ions on pH, gelation kinetics, elastic modulus and gel microstructure was investigated. For all studied systems the time of gelation (tg) had an Arrhenius dependence on temperature, characterised by an activation energy (Ea). The value of Ea depended on the type and concentration of added ions. The elastic modulus of gels was found to be independent of the ion composition, reaching 0.2–0.3 kPa at a protein concentration of 40 g L−1 and 1 kPa at 60 g L−1. At conditions of strong electrostatic repulsion between aggregates, addition of NaCl reduced tg. However, when the repulsions were weak, further addition of NaCl increased tg.

Preparation of a New Material Based on Epoxy Oligomers for Forming Corrosion-Protective Coatings

New adhesive materials based on a single-part hot-cured epoxy adhesive for forming corrosion-protective coatings were obtained. Perfluoropentanoic acid N-methylpiperazide and polyethylenepolyamine were used as curing catalysts. The adhesive compounds in the cured state were characterized by the degree of swelling, gel fraction content, Vickers hardness, elastic modulus, and creep strain; their corrosion-protective properties with respect to low-carbon steel (St. 3) and aluminum–magnesium alloy (AMg-6) were determined. The chemical structure of the organic solvent and amine curing agent largely determines the operation characteristics of the coatings obtained.

A Recrosslinkable Preformed Particle Gel for Conformance Control in Heterogeneous Reservoirs Containing Linear-Flow Features

Summary Preformed particle gels (PPGs) have been successfully applied to control conformance for mature oil fields because of their advantages over conventional in-situ gels. However, field applications have demonstrated that current particle gels cannot efficiently plug open fractures, fracture-like channels, or conduits that exist in many mature oil fields. The objective of this study is to systematically evaluate a new recrosslinkable-PPG (RPPG) product that can be used to efficiently control the conformance for abnormal features. The RPPG can swell to 38 times its initial volume, and the equilibrium swelling ratio is independent of the brine salinity. Temperature and the particle size showed a gradient effect on the swelling rate of the gel. Additionally, the particle gels can recrosslink to form a rubber-like bulky material in the large-opening features after placement that significantly enhances the plugging efficiency. We systematically evaluated the effect of temperature and RPPG swelling ratio on the recrosslinking time, the gel strength after crosslinking, and the gel thermostability. Coreflooding tests were run to test whether RPPG can significantly improve the fracture-plugging efficiency compared with a traditional PPG that cannot recrosslink after pumping. The RPPG can be customized for mature reservoirs with a temperature from 23 to 80°C with a controllable size from tens of nanometers to a few millimeters. The recrosslinking time can be controlled from 2 to 80 hours, depending on the swelling ratio and temperature. The gel elastic modulus after recrosslinking can achieve from 300 to 10 800 Pa, depending on the swelling ratio and the temperature. Coreflooding tests showed that the breakthrough pressure of the recrosslinked RPPG can reach up to 300 psi/ft for a fracture with a 0.2-cm aperture, which is more than five times higher than that of the conventional PPG.

Multiple Thermal Gelation of Hydroxypropyl Methylcellulose and Kappa-Carrageenan Solutions and their Interaction with Salts

The multiple gelation behavior of aqueous solutions of kappa-carrageenan (KCG) and low molecular weight hydroxypropyl methylcellulose (HPMC) was studied with the presence of various salts. Multiple gelation behavior of aqueous solutions of HPMC/KCG/salt mixture were found. The shear viscosity of HPMC/KCG blend increased by one orders of magnitude, while the viscosity of HPMC/KCG/potassium chloride (KCl) mixture increased by three orders of magnitude as compared to HPMC solution at temperatures below apparent gelation. The dynamic elastic modulus of HPMC/KCG blend increased by two orders of magnitude, while the elastic modulus of HPMC/KCG/potassium chloride (KCl) mixture increased by three orders of magnitude as compared to HPMC solution at temperatures below apparent gelation temperature. The gel elastic modulus of the solution blend of HPMC/KCG/salt mixture decreased in the order of KCl &gt; NaCl &gt; CaCl2. Thermal analysis revealed a linear relationship between the depression of melting temperature and the salt concentrations, which is independent of KCG. The free water content computed by enthalpy data showed that free water content decreased with increasing salt concentrations. The secondary peak which typically associated with bound water appeared in the mixture of HPMC and KCG in the presence of KCl. As the concentrations of KCl salt increased, the bound water peak also amplified and lifted to a higher temperature.

Role of sonication pre-treatment and cation valence in the sol-gel transition of nano-cellulose suspensions

Sol-gel transition of carboxylated cellulose nanocrystals has been investigated using rheology, SAXS, NMR and optical spectroscopies to unveil the distinctive roles of ultrasound treatments and addition of various cations. Besides cellulose fiber fragmentation, sonication treatment induces fast gelling of the solution. The gelation is independent of the addition of cations, while the final rheological properties are highly influenced by the type, concentration and sequence of the operations since the cations must be added prior to sonication to produce stiff gels. The gel elastic modulus was found to increase proportionally to the ionic charge rather than the cationic size. In cases where ions were added after sonication, SAXS analysis of the Na+ hydrogel and Ca2+ hydrogel indicated the presence of structurally ordered domains in which water is confined, and 1H-NMR investigation showed the dynamics of water exchange within the hydrogels. Conversely, separated phases containing essentially free water were characteristic of the hydrogels obtained by sonication after Ca2+ addition, confirming that this ion induces irreversible fiber aggregation. The rheological properties of the hydrogels depend on the duration of the ultrasound treatments, enabling the design of programmed materials with tailored energy dissipation response.

Changes in E-cadherin rigidity sensing regulate cell adhesion

Mechanical cues are sensed and transduced by cell adhesion complexes to regulate diverse cell behaviors. Extracellular matrix (ECM) rigidity sensing by integrin adhesions has been well studied, but rigidity sensing by cadherins during cell adhesion is largely unexplored. Using mechanically tunable polyacrylamide (PA) gels functionalized with the extracellular domain of E-cadherin (Ecad-Fc), we showed that E-cadherin-dependent epithelial cell adhesion was sensitive to changes in PA gel elastic modulus that produced striking differences in cell morphology, actin organization, and membrane dynamics. Traction force microscopy (TFM) revealed that cells produced the greatest tractions at the cell periphery, where distinct types of actin-based membrane protrusions formed. Cells responded to substrate rigidity by reorganizing the distribution and size of high-traction-stress regions at the cell periphery. Differences in adhesion and protrusion dynamics were mediated by balancing the activities of specific signaling molecules. Cell adhesion to a 30-kPa Ecad-Fc PA gel required Cdc42- and formin-dependent filopodia formation, whereas adhesion to a 60-kPa Ecad-Fc PA gel induced Arp2/3-dependent lamellipodial protrusions. A quantitative 3D cell-cell adhesion assay and live cell imaging of cell-cell contact formation revealed that inhibition of Cdc42, formin, and Arp2/3 activities blocked the initiation, but not the maintenance of established cell-cell adhesions. These results indicate that the same signaling molecules activated by E-cadherin rigidity sensing on PA gels contribute to actin organization and membrane dynamics during cell-cell adhesion. We hypothesize that a transition in the stiffness of E-cadherin homotypic interactions regulates actin and membrane dynamics during initial stages of cell-cell adhesion.