Growth Of Eggplant Research Articles

The Impact of Grafting with Different Rootstocks on Eggplant (Solanum melongena L.) Growth and Its Rhizosphere Soil Microecology

This study investigated the effects of grafting on eggplant growth, yield, and disease resistance, with a focus on microbial dynamics in the rhizosphere. Eggplant scions were grafted onto rootstocks of wild eggplant and tomato, with self-rooted eggplants serving as controls. Greenhouse experiments were conducted over an eight-month growing period, using standard field practices such as film mulching and integrated water–fertilizer management. High-throughput sequencing was used to analyze the biological properties and microbial community of the rhizosphere soil. Results showed that plants grafted onto ‘Huimei Zhenba’ and ‘Torvum’ rootstocks yielded up to 36.89% more than self-rooted controls, achieving yields of 4619.59 kg and 4399.73 kg per 667 m², respectively. The disease incidence of bacterial wilt was reduced to as low as 3.33% in the ‘Huimei Zhenba’ treatment, compared to 55.56% in non-grafted controls. Additionally, grafted plants exhibited increased stem diameter and chlorophyll content, with the TL/HM combination reaching 54.23 ± 3.17 SPAD units. The enhanced microbial biomass of carbon, nitrogen, and phosphorus, particularly in the TL/HM treatment (377.59 mg/kg, 28.31 mg/kg, and 36.30 mg/kg, respectively), supports a more nutrient-rich rhizosphere environment. Moreover, soil enzyme activities, such as β-glucosidase and phosphatase, were significantly higher in grafted plants, enhancing nutrient cycling and potentially increasing resistance to pathogens. Overall, grafted eggplants demonstrated enhanced soil microbial biomass, enzyme activity, and a more diverse microbial community, which are critical factors contributing to the improved yield and disease resistance observed in grafted crops.

Selected Actinomycetes as Potential Biostimulant of Eggplant (Solanum melongena L) in Mali

Aims: This study aims to formulate at least one efficient biostimulant based on actinomycetes that promotes good eggplant growth in Mali. Study Design: The experimental design was a completely randomized block design with three replications and four treatments randomly distributed. The treatments were three Actinomycete strains (Act, S1 and St1) and a non inoculated control and the mode of application (inoculation or spraying) of these treatments were considered as blocking factor. Place and Duration of Study: The trials were conducted in Bamako, in the Greenhouse of the LaboREM-Biotech situated on the colline of Badalabougou. Methodology: Eggplant seeds inoculated or not with Actinomyces sp. Ts1, Actinomyces sp. S1 and Actinomyces sp. Act; were randomly sown in plastic pots filled with approximately 2.50 kg of air-dried soil. The first block sown with coated seeds and the second with non-inoculated seeds and seedlings from them pulverized. Leaf length, leaf area, leaf diameter, leaf area, leaf fresh weight, leaf dry weight, root fresh weight, root dry weight, plant height and root length were determined. Results: Eggplant seed inoculated with all the tested Actinomycetes strains had significantly enhanced leaf length, leaf diameter, leaf area, leaves fresh weight. The highest results were obtained with the strain Act, followed by strain St1. Sprayed treatment improved eggplant leaf length, leaf diameter, leaf area, leaves fresh weight, leaves dry weight, root fresh weight, root dry weight; compared to the coated treatment Conclusion: Field application of actinomyctes may reduce production cost, by increasing eggplant growth and fruit yield while reducing production cost. The present work showed that Act and Ts1 using spray method were best suited for eggplant. Actinomyces sp. Act produced the highest leaf length, leaf diameter, leaf area, leaves fresh weight. Therefore, Actinomyces sp. Act is suggested as bioinoculant for eggplant.

Construction of Greenhouse for Horticultural Cultivation with Soil Media and Hydroponics

Theneral background greenhouse technology is increasingly recognized as an efficient solution for sustainable agricultural practices, especially in regions with limited fertile land. Specific background In Kedungpeluk Village, where the majority of residents work as fish farmers, there are also unused and arid lands. These underutilized spaces provide an opportunity for greenhouse-based horticultural cultivation using soil media and hydroponic technology. Knowledge gap however, limited knowledge and infrastructure have prevented local farmers from effectively utilizing these lands for sustainable crop production. Aims this community engagement project aims to build and utilize a greenhouse system to cultivate horticultural plants, focusing on optimizing both soil and hydroponic methods. Results the project successfully transformed barren land into productive greenhouses that facilitate the growth of tomatoes, chilies, eggplants, pak choy, and water spinach. The greenhouse structure, designed with transparent roofing, enhances sunlight exposure, promoting optimal plant growth. Both soil and hydroponic methods showed potential for increasing crop yields, with soil cultivation being more flexible but requiring extra care for pests, while hydroponics provided faster results with minimal weed and pest interference. Novelty this project introduces a dual-method approach combining traditional soil cultivation with modern hydroponic technology in a controlled greenhouse environment. Implications the findings highlight that greenhouse farming is a viable, modern agricultural solution for rural communities, offering increased productivity and quality crops, while also addressing the challenges of limited fertile land and changing climate conditions. This project serves as a model for sustainable agriculture in similar settings. Highlights: Dual-method approach: Combining soil and hydroponic techniques in a greenhouse. Efficient land use: Transforming unused arid land for productive horticulture. Controlled environment: Optimizing plant growth through sunlight, temperature, and humidity control. Keywords: Greenhouse, Horticulture, Soil Media, Hydroponics, Sustainable Agriculture

Employing Titanium Dioxide Nanoparticles as Biostimulant against Salinity: Improving Antioxidative Defense and Reactive Oxygen Species Balancing in Eggplant Seedlings.

Salinity is a major abiotic stress that affects the agricultural sector and poses a significant threat to sustainable crop production. Nanoparticles (NPs) act as biostimulants and significantly mitigate abiotic stress. In this context, this experiment was designed to assess the effects of foliar application of titanium dioxide (TiO2) nanoparticles at 200 and 400 ppm on the growth of eggplant (Solanum melongena) seedlings under moderate (75 mM) and high (150 mM) salinity stress. The TiO2-NPs employed were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and scanning electron microscopy (SEM) analysis. The seedlings were assessed physiologically, growth-wise, and biochemically. The seedlings were significantly affected by their physiological attributes (Fv'/Fm', Fv/Fm, NPQ), growth (root length, shoot length, number of leaves, fresh biomass, dry biomass, leaf greenness), antioxidative enzymes (SOD, POD, CAT, APx, GR), stress indicators (H2O2, MDA), and toxic ion (Na+) concentrations. The maximum decrease in physiological and growth attributes in eggplant seedling leaves was observed with no TiO2-NP application at 150 mM NaCl. Applying TiO2-NPs at 200 ppm showed significantly less decrease in Fv'/Fm', root length, shoot length, number of leaves, fresh biomass, dry biomass, and leaf greenness. In contrast, there were larger increases in SOD, POD, CAT, APx, GR, and TSP. This led to less accumulation of H2O2, MDA, and Na+. No significant difference was observed in higher concentrations of TiO2-NPs compared to the control. Therefore, TiO2-NPs at 200 ppm might be used to grow eggplant seedlings at moderate and high salinity.

Irrigation and oil palm empty fruit bunch mulch enhance eggplant growth, radiation interception and dry matter yield

Organic mulching is a well-known management practice that conserves soil water and nutrients as well as increases crop yield. Nonetheless, research on combined organic mulching using oil palm empty fruit bunch (EFB) and irrigation is limited. Field-based experiments were conducted over three seasons to test the sole and combined effects of EFB as organic mulch and irrigation on the growth, total dry matter yield (TDMY), accumulated intercepted photosynthetically active radiation (AIPAR), and radiation use efficiency (RUE) of African eggplant (Solanum aethiopicum L.) in a low fertile tropical sandy clay loam soil. Air-dried EFB was used as an organic mulch by spreading it on the soil surface at rates of 0 (EFB0), 20 (EFB20), and 40 t ha−1 (EFB40), and either fully-irrigated (I100), deficit-irrigated (I40), or non-irrigated (I0). The I100 plots were irrigated to field capacity (FC) every 3–4 days based on PR2 Profile Probe measurements and the resultant irrigation volume supplied to the plants via drip irrigation tubes. The I40 plots received 40 % of the water given to the I100 plots, and the I0 plots were solely rain-fed. At the end of the third season, the 40 t ha−1 EFB-mulch increased soil pH, electrical conductivity (EC), soil organic carbon, potassium, cation exchange capacity, and the soil’s specific surface area. In the first season, all the measured eggplant growth and yield parameters were neither responsive to irrigation only, EFB-mulch only, or both. In the second and third seasons, the EFB20 and EFB40 treatments significantly (p < 0.05) increased leaf chlorophyll content index (LCCI), photosystem II (Fv/Fm ratio), absolute performance index (PIabs), TDMY, AIPAR, and RUE compared to the non-mulched control treatment. Soil pH was high in the EFB-mulched plots and correlated positively with TDMY and AIPAR. The I100 significantly improved LCCI, Fv/Fm, PIabs, and TDMY during the second season. In the third season, a highly significant interaction between irrigation and mulching was detected on TDMY, AIPAR, RUE, LCCI, Fv/Fm ratio, and pH (H2O). This indicated a positive effect on soil nutrient availability especially phosphorus as TDMY and AIPAR correlated with soil pH. The I100 and I40 significantly increased AIPAR by 48.1 % and 37.2 %, and RUE by 26.7 % and 11.0 %, respectively, compared to I0 during the third season. The total dry matter yield of the African eggplant was enhanced by EFB-mulch, with the effect increasing over up to three growing seasons, especially when combined with irrigation during dry periods.

YOLOv5s-BiPCNeXt, a Lightweight Model for Detecting Disease in Eggplant Leaves.

Ensuring the healthy growth of eggplants requires the precise detection of leaf diseases, which can significantly boost yield and economic income. Improving the efficiency of plant disease identification in natural scenes is currently a crucial issue. This study aims to provide an efficient detection method suitable for disease detection in natural scenes. A lightweight detection model, YOLOv5s-BiPCNeXt, is proposed. This model utilizes the MobileNeXt backbone to reduce network parameters and computational complexity and includes a lightweight C3-BiPC neck module. Additionally, a multi-scale cross-spatial attention mechanism (EMA) is integrated into the neck network, and the nearest neighbor interpolation algorithm is replaced with the content-aware feature recombination operator (CARAFE), enhancing the model's ability to perceive multidimensional information and extract multiscale disease features and improving the spatial resolution of the disease feature map. These improvements enhance the detection accuracy for eggplant leaves, effectively reducing missed and incorrect detections caused by complex backgrounds and improving the detection and localization of small lesions at the early stages of brown spot and powdery mildew diseases. Experimental results show that the YOLOv5s-BiPCNeXt model achieves an average precision (AP) of 94.9% for brown spot disease, 95.0% for powdery mildew, and 99.5% for healthy leaves. Deployed on a Jetson Orin Nano edge detection device, the model attains an average recognition speed of 26 FPS (Frame Per Second), meeting real-time requirements. Compared to other algorithms, YOLOv5s-BiPCNeXt demonstrates superior overall performance, accurately detecting plant diseases under natural conditions and offering valuable technical support for the prevention and treatment of eggplant leaf diseases.

Effects of different water stresses under subsurface infiltration irrigation on eggplant growth and water productivity

Soil moisture deficiency limits eggplant growth and affects the yield and quality. Identifying an appropriate deficit irrigation strategy to enhance irrigation water productivity is an urgent task. Additionally, the dynamic response of eggplants to water stress at various phenological stages needs further clarification. In this study, eggplants were used as experimental materials, with field capacity (FC) (Senyigit et al.) serving as the irrigation standard. Two levels of deficit irrigation treatments (60 % FC and 50 % FC) were applied at three key phenological stages of eggplant (seedling stage, flowering and fruiting, and prime fruit stage). A randomized block design was used with non-deficit irrigation (80 % FC) during the phenological stages as the control (W1), resulting in seven treatments, each with three replicates. The results showed that at the same phenological stage, plant height, stem diameter, leaf area index (LAI), dry matter accumulation, total fresh root weight, total root length, total root surface area, and total root volume decreased as the irrigation threshold was lowered. The content of vitamin C, soluble sugars, souble proteins, and crude fiber increased as the irrigation threshold decreased. Leaf photosynthetic parameters (photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), and intercellular CO2 concentration (Ci)) were positively correlated with the irrigation threshold. The W1 treatment had the highest yield (89.1 t·ha−1 in 2018; 88.8 t·ha−1 in 2019), followed by the W2 treatment (86.1 t·ha−1 in 2018; 85.6 t·ha−1 in 2019). Water deficit during the prime fruit stage (W6, W7) had the most significant impact on yield. W1 had the highest water productivity (19.4 kg·m−3 in 2018; 19.2 kg·m−3 in 2019), with no significant difference from W2 (19.4 kg·m−3 in 2018; 19.0 kg·m−3 in 2019). Using the Jensen function, the water sensitivity of eggplants at different phenological stages was ranked as follows:prime fruit stage > flowering and fruiting stage > seedling stage. Path analysis indicated that dry matter accumulation, single fruit weight, and single plant yield were important indicators affecting yield. Considering irrigation amount, eggplant growth, fruit yield and quality, and water productivity, the optimal deficit irrigation thresholds for eggplants in arid regions are determined to be 60 % FC during the seedling stage, 60 % FC during the flowering and fruiting stage, and 80 % FC during the prime fruit stage. The findings of this study offer valuable insights for precision agricultural irrigation practices.

Growth, yield and quality response of Eggplant (Solanum melongena L.) to blended NPSB fertilizer rates and intra-row spacing in Boloso Bombe district, Wolaita zone, South Ethiopia

Eggplant is the most important fruit vegetable crop in many countries, including Ethiopia. In spite of this, its production is hindered by low nutrient availability in soil and suboptimal intra-row spacing. In order to address these issues, a field investigation took place in the Wolaita zone during 2022/23 growing season to evaluate the effects of various concentrations of blended NPSB fertilizer and intra-row spacing on the growth, yield, and quality of eggplants. Five various blended NPSB fertilizer rates (0, 50, 100, 150, and 200 kg ha−1) and three intra-row spacing distances (30, 40, and 50 cm) were used in the investigation, which was designed as a randomized complete block with three replicates. Based on an analysis of variance, it was discovered that the main effects of NPSB fertilizer rates and intra-row spacing significantly (p < 0.01) affected many of parameters, including dry matter content, total soluble solids, fruit number, fruit length, and days to 50 % flowering. Additionally, the interaction effect between NPSB fertilizer and intra-row spacing significantly (p < 0.05) impacted the number of days to first fruit harvest, plant height, leaf area, branch number, leaf number, fruit diameter, fresh fruit yield, marketable fresh fruit yield, unmarketable fresh fruit yield, and total fresh fruit yield. According to the current investigation, the highest marketable fresh fruit yield (121.04 t ha−1) was obtained by 150 kg ha−1 NPSB with 40 cm intra-row spacing. These findings suggest that 150 kg ha−1 of blended NPSB fertilizer with 40 cm distance between plant spacing is optimal for eggplant cultivation in the study area and analogous agro-ecological settings. This optimized approach can effectively support eggplant growers in maximizing both yield and quality outcomes.

Grafting increases superoxide dismutase and catalase activity to overcome the impact of the two-spotted spider mite on eggplant growth and productivity

Grafting vegetable crops is considered a valuable tool for improving the yield and quality of fruits under various stress conditions. This study aimed to assess the influence of grafting eggplant Solanum melongena (cv. A338) onto Solanum torvum rootstock (cv. STT3) on the incidence of the two-spotted spider mite Tetranychus urticae (TSSM). An experimental study was conducted to investigate the defense response elicited, as assessed by measuring the activities of antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT), as well as hydrogen peroxide (H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;) and chlorophyll contents in grafted and non-grafted eggplants under different TSSM infestation levels (10, 30 and 50 adults per plant). In addition, the effect of grafting on plant growth and yield of eggplant was evaluated. According to the findings of the present study, grafted plants had a lower density of TSSM than non-grafted plants. The number of eggs, nymphs, and adults on grafted plants was 40.11%, 31.71%, and 27.54%, respectively, lower than on non-grafted plants. With increasing TSSM density, the activities of CAT and SOD were increased at varying degrees. In terms of SOD activity, the grafted plants outperformed both the non-grafted scion and rootstock at all TSSM densities. In addition, the results demonstrated that plants with greater CAT activity were able to maintain a lower H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; content and vice versa. The lower negative impacts on photosynthetic pigments demonstrated that grafted plants on S. torvum were more resistant to mite-induced damage to chlorophyll and carotenoids as mite density elevated. The grafted plants were superior in vegetative growth, and the total fruit weight was found to be two times higher than non-grafted plants. The features modified by grafting, such as the increased activities of SOD and CAT, can alleviate the impact of TSSM. Therefore, grafting could constitute a valuable tool when combined with other strategies of Integrated Pest Management (IPM) in the two-spotted spider mite control programs.

Utilization of Tasikmadu Starfruit Waste Compost for The Growth and Yield of Purple Eggplant (Solanum melongena L.) In Supporting Sustainable Agriculture

Based on the literature study, the demand for eggplant in East Java is increasing, encouraging farmers to increase productivity by using chemical fertilizer. In the long term, this practice could harm the environment. This study was conducted to obtain the right method and dosage of compost made from starfruit waste for the growth and yield of eggplant. The study was designed using a Factorial Randomized Block Design. The first factor was organic fertilizer with 5 levels, namely: Soil + goat manure 1:1, Soil + compost from composter 1:1; Soil + compost from bio-pore1:1; Soil + goat manure + compost from composter 1:1:1, and Soil + goat manure + compost from bio-pore 1:1:1. The second factor was NPK fertilizer with 4 levels, namely: 0 gram/liter, 1 gram/liter; 2 gram/ liter; dan 3 gram/liter. There were three replications for each treatment. The results showed that the best organic fertilizer was a combination of manure and compost, from composter and bio-pore, which had the highest result for plant height, number of leaves, biomass increase, and the number of eggplant fruits. The best concentration of NPK fertilizer for eggplant growth and physiology was 3 grams per liter.

Efficacy of Vermicast from Local Earthworms as Growing Media of Eggplant (Solanum melongena L.)

The study aimed to determine the effectiveness of vermicast from local earthworms as a growing medium for eggplant, with the goal of exploring the potential of organic fertilizers in boosting vegetable yields and promoting sustainable agriculture practices. It investigated the efficacy of vermicast derived from local earthworms in enhancing seed germination and seedling growth of eggplant using a randomized complete block design with four treatments and three replications. The experiment compared garden soil, vermicast, carbonized rice hull, and combinations of these growing media, evaluating germination percentage, seedling height, number of leaves, and leaf area. Results showed significant differences among treatments, with vermicast and carbonized rice hull exhibiting superior performance compared with garden soil. Carbonized rice hull showed the highest germination percentage in the second and third weeks, while vermicast from local earthworms led to the tallest seedlings and highest leaf area in subsequent weeks, suggesting its preference for cultivating eggplant seedlings. The study highlights the potential of vermicast from local earthworms as a sustainable and effective organic soil amendment for enhancing eggplant growth.

Plant protein hydrolysate and arbuscular mycorrhizal fungi synergistically orchestrate eggplant tolerance to iodine supply: A two-year study

Biofortification is a promising strategy to overcome iodine (I) deficiencies in the world population. However, since iodine is not essential for plants, its administration may cause phytotoxicity issues. The objective of this work was to evaluate the effects of two biostimulants [arbuscular mycorrhizal fungi (AM) and plant protein hydrolysates (PH)], used alone or in combination, on eggplant plants treated with three different I doses (0, 300 or 600 mg L−1). Results underlined that the highest I dosage significantly reduced plant growth, yield, fruit dry matter content, chlorophylls and stomatal conductance compared to the control. Whereas, I increased total anthocyanins, chlorogenic acid, antioxidant activity, fruit I concentration, proline, H2O2 and malondialdehyde of the biofortified plants compared to non-biofortified plants. The biostimulants enhanced eggplant growth and yield, fruit dry matter content, chlorophylls, total anthocyanins and chlorogenic acid compared to the control, especially when both biostimulants were supplied. Interestingly, it was often recorded an interaction between I and biostimulants, suggesting that PH and AM had a buffer effect on I toxicity, specifically when combined (PH+AM). Overall, our study pointed out that the mutual use of microbial (AM) and non-microbial (PH) biostimulants and the application of 300 mg I L−1 might be an helpful approach to relieve the detrimental effects of high I dosages and, simultaneously, to increase crop yield and fruit quality of eggplant.

The Effect of Using NPK Fertilizer and Liquid Organic Fertilizer Vegetable Waste on the Vegetative Growth of Purple Eggplant (Solanum melongena L.)

An key factor in promoting the growth and development of plants is fertilizer. Both organic and inorganic fertilizers can be used for fertilization. If inorganic fertilizers are applied over an extended period of time without the incorporation of organic matter, the soil's quality may be compromised, leading to suboptimal plant development. The purpose of this study is to ascertain the optimal fertilizer dose for purple eggplant growth response, as well as the effects of NPK fertilizer, liquid organic fertilizer made from vegetable waste, and a combination of both on the vegetative growth of purple eggplant plants. A two-factor Complete Random Design was employed in this study, with three replicates and five treatment levels for each of the liquid organic fertilizer made from vegetable waste and NPK fertilizer treatment. The Anova two-way test was used to examine the data, and then the DMRT test was performed. The application of liquid organic fertilizer made from vegetable waste and the combination of the two fertilizers had a real effect on plant height, leaf area, wet weight, and dry weight but no real effect on the number of leaves, according to the results. On the other hand, the application of NPK fertilizer had a real effect on plant height, number of leaves, leaf area, wet weight, and dry weight. The results of the DMRT test (5%) indicated that the optimal dosage for plant height, wet weight, and dry weight was 1.5 grams of NPK and 10 ml of liquid organic fertilizer made from vegetable waste; for the number of leaves, it was 1.5 grams of NPK and 2 grams, and for the area of leaves, it was 1 gram of NPK.

Mustard (Brassica rapa) and Eggplant (Solanum melongena) Cultivation in Agrivoltaic System in Coastal Area, Case Study of Baron Technopark, Yogyakarta

Agrivoltaic is a system developed from plant cultivation and photovoltaic installations. Opportunities for creating this system in Indonesia are still quite large, including in the Gunungkidul Yogyakarta area. This study aimed to determine the growth response and yield of mustard greens and eggplant in the Agrivoltaic system in Baron, Gunungkidul, Yogyakarta, as a preliminary study of the use of photovoltaic for plant cultivation activities. The research was conducted in a photovoltaic installed in the Baron Techno Park area, BRIN, Gunungkidul Regency, Yogyakarta. An unpaired T-test design was used in this research, which compared the growth and yield of mustard greens and eggplant under and outside the solar panels. The development of mustard and eggplant plants showed quite a high variation between rows below and outside the panel. Therefore, the rows were also used as a treatment in this research. The results showed that mustard greens and eggplants can be grown in agrivoltaic systems. The location of the plants (below and outside the panel) did not affect the growth and yield of mustard plants, while eggplant did. The position of the row inside and outside the panel also affects the growth and yield of mustard greens and eggplant. Mustard and eggplant plants planted outside the panels will be harvested 15 days faster than those under the panels

Increasing the Growth and Production of Eggplant (Solanum melongena L) by Providing Wood Biochar

The cultivation of eggplant (Solanum melongena L.) is a significant aspect of horticultural production in Indonesia. However, the productivity of eggplant remains minimal due to inadequate cultivation practices. An encouraging approach involves utilizing wood biochar as a supplement to improve soil fertility and promote plant development. This study aimed to assess the impact of varying levels of wood biochar application on the development and productivity of eggplant. The study utilized a Randomized Block Design (RBD) to investigate the effects of four different treatments: no biochar, 50 g of biochar per polybag, 100 g of biochar per polybag, and 150 g of biochar per polybag. The findings indicated that the incorporation of wood biochar had a notable impact on the growth and yield of the plants, as evidenced by the increase in plant height, leaf width, fruit weight per fruit, number of fruits per plant, and fruit weight per plant. Among the different treatments, B3 exhibited the most favorable outcomes. The analysis of variance (ANOVA) revealed that there were statistically significant differences between the various treatments (p &lt; 0.05). In summary, using wood biochar has proven to successfully improve eggplant yield in Indonesian soil.

Restoration of fumigated soil biota with plant growth-promoting rhizobacteria to counteract Meloidogyne incognita (Tylenchida: Heteroderidae) boosts eggplant growth and defenses

This study investigated the effectiveness of two plant growth-promoting rhizobacteria (PGPR) formulations, Bio Arc® 6% WP (BA, Bacillus megaterium) and BECTO Grow Roots® (BGR; Serratia marcescens, Pseudomonas putida, and P. fluorescens). Additionally, it focused on naturally infested soil that had undergone metam potassium fumigation to reduce root-knot nematode (RKN), specifically Meloidogyne incognita. It aimed to monitor eggplant response to soil biodiversity changes for 60 days of the experiment under greenhouse conditions. Our data showed that soil fumigation eliminated nematodes and reduced other microorganisms significantly. In sterilized soil, plant fresh root weight significantly decreased after the elimination of soil biota. Moreover, BGR achieved the highest plant growth vigor [fresh root (18.48%), fresh shoot (23.52%), and fresh plant weight (21.03%)], followed by BA [fresh root (11.98%), fresh shoot (20.77%), and fresh plant weight (16.38%), respectively]. Plant growth traits increased significantly following the second reinoculation of PGPR after a month. Also, the same data trend was observed in treatments inoculated with RKN + PGPR. Furthermore, BGR treatments exhibited consistent efficacy in controlling nematode infection, maintaining superior vegetative growth compared to BA treatments over time. However, BA treatments displayed a decline in plant growth when faced with repeated nematode infection. The range of gall, egg mass, and second-stage juvenile (J2s) numbers diminished after inoculation-infected plants with RKN and PGPR compared with infected plants alone. Biochemical changes in eggplant roots cultivated in sterilized soil exposed significant enhancement in total sugars, protein, and nonenzymatic antioxidants (flavonoids and phenolic content), with antioxidant activity enhanced upon inoculation of PGPR and RKN. Oppositely, the levels of enzymatic antioxidants (catalase, peroxidase, and superoxide dismutase) decreased significantly with single and double PGPR application. However, the decline was less pronounced in the BGR treatments than in BA. In contradiction of oxidative enzymes, reinoculated PGPR + RKN multiplied significantly in reinfested RKN treatments. Our findings support the use of PGPR combinations, in particular, for preventive inoculation against M. incognita. They also enhance plant growth-promoting factors and stimulate plant defenses against inevitable soil reinfestation following soil fumigation.

Physiological responses and initial growth of eggplant under nutrient exclusion from nutrient solution

Physiological responses and initial growth of eggplant under nutrient exclusion from nutrient solution

Amelioration of phytotoxic impact of biosynthesized zinc oxide nanoparticles: Plant growth promoting rhizobacteria facilitates the growth and biochemical responses of Eggplant (Solanum melongena) under nanoparticles stress

The consistently increasing use of zinc oxide nanoparticles (ZnONPs) in crop optimization practices and their persistence in agro-environment necessitate expounding their influence on sustainable agro-environment. Attempts have been made to understand nanoparticle-plant beneficial bacteria (PBB)- plant interactions; the knowledge of toxic impact of nanomaterials on soil-PBB-vegetable systems and alleviating nanotoxicity using PBB is scarce and inconsistent. This study aims at bio-fabrication of ZnONPs from Rosa indica petal extracts and investigates the impact of PBB on growth and biochemical responses of biofertilized eggplants exposed to phyto-synthesized nano-ZnO. Microscopic and spectroscopic techniques revealed nanostructure, triangular shape, size 32.5 nm, and different functional groups of ZnONPs and petal extracts. Inoculation of Pseudomonas fluorescens and Azotobacter chroococcum improved germination efficiency by 22% and 18% and vegetative growth of eggplants by 14% and 15% under NPs stress. Bio-inoculation enhanced total chlorophyll content by 36% and 14 %, increasing further with higher ZnONP concentrations. Superoxide dismutase and catalase activity in nano-ZnO and P. fluorescens inoculated eggplant shoots reduced by 15–23% and 9–11%. Moreover, in situ experiment unveiled distortion and accumulation of NPs in roots revealed by scanning electron microscope and confocal laser microscope. The present study highlights the phytotoxicity of biosynthesized ZnONPs to eggplants and demonstrates that PBB improved agronomic traits of eggplants while declining phytochemicals and antioxidant levels. These findings suggest that P. fluorescens and A. chroococcum, with NPs ameliorative activity, can be cost-effective and environment-friendly strategy for alleviating NPs toxicity and promoting eggplant production under abiotic stress, fulfilling vegetable demands.

Alleviation of heat stress by ACC Deaminase and Exopolysaccharide Producing Plant-Growth-Promoting Rhizobacteria in Solanum melongena L.

Background: The current study was carried out to assess the alternative role of high temperature tolerant plant growth promoting rhizobacteria (PGPR) in alleviation of heat stress adverse effect in eggplant.Methods: PGPR was assessed for PGP potentialities and investigated for1‐aminocyclopropane‐1‐carboxylate (ACC deaminase), and exopolysaccharide under normal and high temperature environment. The impact of PGPR application on plant physiology and biochemistry was investigated under normal and high temperature environments and plant growth regulators were evaluated using High Performance Liquid Chromatography (HPLC).Results: A significant impediment for morphological and physiological parameters of eggplant cultivars was observed during heat stress in absence of PGPR inoculation. The results showed that ACC‐deaminase produced by PGPR boosted up significantly the extracellular polymeric substances (EPS) accumulation, conversion of ACC into α‐ketobutyrate and ammonia, and reduce consequently the impact of high temperature on eggplant development.Conclusion: PGPR inoculation showed an alternative strategy to improve eggplant growth and development under high temperature condition in order to preserve agriculture sustainability and healthy crops.Keywords: ACC‐deaminase; Extracellular polymeric substances; Heat stress; PGPR; Solanum melongena

Microbial consortium with multifunctional attributes for the plant growth of eggplant (Solanum melongena L.).

In the past few decades, the pressure of higher food production to satisfy the demand of ever rising population has inevitably increased the use synthetic agrochemicals which have deterioration effects. Biostimulants containing beneficial microbes (single inoculants and microbial consortium) were found as an ideal substitute of synthetic chemical fertilizers. In recent years, microbial consortium is known as a better bioinoculant in comparison to single inoculant bioformulation because of multifarious plant growth-promoting advantages. Looking at the advantageous effect of consortium, in present investigation, different bacteria were isolated from rhizospheric soil and plant samples collected from the Himalayan mountains on the green slopes of the Shivaliks, Himachal Pradesh. The isolated bacteria were screened for nitrogen (N) fixation, phosphorus (P)solubilization and potassium (K) solubilization plant growth promotingattributes, and efficient strains were identified through 16S rRNA gene sequencingand BLASTn analysis. The bacteria showing a positive effect in NPK uptake were developed as bacterial consortium for the growth promotion of eggplant crop. A total of 188 rhizospheric and endophytic bacteria were sorted out, among which 13 were exhibiting nitrogenase activity, whereas 43 and 31 were exhibiting P and K solubilization traits, respectively. The selected three efficient and potential bacterial strains were identified using 16S rRNA gene sequencing as Enterobacter ludwigii EU-BEN-22 (N-fixer; 35.68 ± 00.9 nmol C2H4 per mg protein per h), Micrococcus indicus EU-BRP-6 (P-solubilizer; 201 ± 0.004 mg/L), and Pseudomonas gessardii EU-BRK-55 (K-solubilizer; 51.3 ± 1.7 mg/mL), and they were used to develop a bacterial consortium. The bacterial consortium evaluation on eggplant resulted in the improvement of growth (root/shoot length and biomass) and physiological parameters (chlorophyll, carotenoids, total soluble sugar, and phenolic content) of the plants with respect to single culture inoculation, chemical fertilizer, and untreated control. A bacterial consortium having potential to promote plant growth could be used as bioinoculant for horticulture crops growing in hilly regions.