Egg Transport Research Articles

The role of larval transport on recruitment dynamics of red mullet (Mullus barbatus) in the Central Mediterranean Sea

Recruitment success depends on external forcing mechanisms such as ocean currents that affect the transport of eggs and larvae to favorable habitats. In this study, we investigated the role of larval transport in the recruitment of Mullus barbatus in the Central Mediterranean Sea by modeling the recruits' abundance as a function of both spawning stock size and dispersal rates of the species’ early life stages. Our analysis involved twenty years of data on recruits and spawners abundance obtained from scientific trawl surveys, and data on larval dispersal rates derived from a combination of actualized published sources and original data. By calculating the estimates of retention, import and uniformity of the contribution of the spawning areas distributed among different Geographical Sub Areas (GSAs) in the Sicilian nurseries, we assessed their contribution to recruitment using modified Ricker stock size-recruits models. In particular, our results show that a high uniform contribution from spawning areas within GSA16, mainly related to the oceanographic patterns promoting larval retention, together with spawners abundance, significantly reduced the variability of red mullet recruitment. We further highlighted that when switching from a higher to a lower level of evenness of contribution to the recruit population from different spawning areas in the GSA16, the expected spawning stock abundance per recruit for a given fishing pattern can suffer a rapid short-term decline, which is likely to have negative consequences for stock assessment and management decisions. Our results suggest that larval transport plays a crucial role in explaining the interannual variability of recruitment, thereby contributing to a better understanding of stock size variation. Additionally, our study enhances the understanding of the spatial dynamics involved in the recruitment of this species, which is of increasing interest within fisheries management frameworks.

Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination

The rheological properties of the extracellular fluid in the female reproductive tract vary spatiotemporally, however, the effect on the behaviour of epithelial cells that line the tract is unexplored. Here, we reveal that epithelial cells respond to the elevated viscosity of culture media by modulating their development and functionality to enhance cilia formation and coordination. Specifically, ciliation increases by 4-fold and cilia beating frequency decreases by 30% when cells are cultured at 100 mPa·s. Further, cilia manifest a coordinated beating pattern that can facilitate the formation of metachronal waves. At the cellular level, viscous loading activates the TRPV4 channel in the epithelial cells to increase intracellular Ca2+, subsequently decreasing the mitochondrial membrane potential level for ATP production to maintain cell viability and function. Our findings provide additional insights into the role of elevated tubal fluid viscosity in promoting ciliation and coordinating their beating—a potential mechanism to facilitate the transport of egg and embryo, suggesting possible therapeutic opportunities for infertility treatment.

Northeast Arctic haddock (Melanogrammus aeglefinus) spawning grounds and drift to nursery areas in the Barents Sea

AbstractThe Northeast Arctic stock of haddock (Melanogrammus aeglefinus) is the most northerly stock of the species. It is one of the largest haddock stocks but not the most studied. Similar to the more extensively studied sympatric cod (Gadus morhua) and herring (Clupea harengus) stocks, it relies on Atlantic currents for egg and larvae transportation from spawning grounds to nursery areas in the Barents Sea. The exact locations of the spawning grounds have not been known and the maps that have been published differ substantially. Here, we present results from the first survey dedicated to exploring the spawning habitat of Northeast Arctic (NEA) haddock, and a particle drift and larvae growth model with physical forcing from a high‐resolution ocean model. Gadoid eggs, later identified as haddock by DNA analyses, as well as spawning individuals were sampled at the survey. Spawning haddock was found in Atlantic water in temperatures 3.6–6.2°C and depths of 240–560 m. Sampled eggs were mostly in early development stages, suggesting that they were largely spawned in the surveyed area. Using the drift model, we found that most of the modelled particles released as eggs at our surveyed area ended up in the nursery area in the Barents Sea. A crude estimate of the spawning stock biomass based on the survey suggests that the most updated spawning ground map of NEA haddock might be too restricted in its extent. Particle drift and growth with physical forcing for the years 2012–2022 indicated small interannual variation in aberrant drift, and that growth driven by ambient temperature could be important in explaining interannual variation in haddock recruitment.

The effect of transport temperature and duration on survival and growth of house cricket nymphs and eggs

Abstract The transport of live insects is becoming increasingly common within the food and feed industry. However, very little information is available as to its effects on the insects themselves. The aim of this study was to monitor how variation in transport conditions, such as duration of transport and external temperature and humidity, directly and indirectly affect the survival and growth of nymphs of the house cricket, Acheta domesticus. In addition, we investigated the effect of external temperature on the viability of eggs upon completion of a transport event. We investigated how four different temperatures (5 °C, 15 °C, 25 °C, 35 °C) and four possible transport durations (1, 2, 3, 6 days) affected survival and growth of the cricket nymphs. To gain insight into the potential transport of eggs, its effect on egg hatch-rate was studied by storing eggs at three different temperatures (6 °C, 18 °C and 30 °C) before moving the eggs to optimal hatching conditions. We found that humidity remained constant inside the transport boxes and was different from the exterior humidity. Temperature had a significant effect on nymph survival and growth. The extreme temperatures negatively affected nymph survival, and increased temperature was correlated with increased growth. The development of eggs was also sensitive to temperature, and transport of eggs at suboptimal temperatures prolonged incubation time considerably. Finally, based on the results of this study, we discuss temperature optimisation as the most critical factor to consider during transport of live house cricket nymphs and eggs.

Population connectivity of small yellow croaker (Larmichthys polyactis) in the southern Yellow Sea: Implications from multiple otolith-based approaches

Connectivity is a fundamental aspect governing the viability of marine species populations, particularly during their early life stage. However, a dearth of comprehensive studies exists regarding connectivity patterns in Larmichthys polyactis within the southern Yellow Sea region. To address this gap, we conducted a thorough investigation into the population connectivity of L. polyactis collected from Haizhou Bay (HZB) and Lvsi (LS) fishing grounds, employing a suite of otolith-based methodologies. Initially, our analysis of otolith microstructure revealed a notable incubation peak occurring between early and mid-April for juvenile L. polyactis sampled in HZB, despite the sea surface temperature (SST) during that period not aligning with the known spawning requirements of L. polyactis. By juxtaposing the spawning period and associated SST conditions of L. polyactis across different fishing grounds, we deduced a high likelihood that the HZB samples originated from the LS. Subsequently, we investigated the stable isotope ratios (δ13C and δ18O) of L. polyactis collected in HZB, LS, and offshore locations, focusing on the core zone indicative of the early life stage. Statistical analysis using Dunn’s multiple comparison test revealed that no significant disparity in otolith δ13C and δ18O values between HZB and LS samples (P > 0.01). However, these values were notably lower than those observed I n offshore samples (P < 0.01). Furthermore, through otolith morphological analysis, we identified two distinct aggregations within the HZB samples, one aligning with the entirety of the LS samples and another representing the remaining HZB sample subset. This finding provides additional support for the population connectivity between HZB and LS stocks. Drawing upon these findings, we posit the existence of a a mixed population of L. polyactis within HZB, likely resulting from the transportation of eggs or larvae originating from LS to HZB via prevailing northward currents. An enhanced understanding of connectivity dynamics among L. polyactis holds significant implications for the formulation of effective management strategies aimed at bolstering stock recovery efforts.

Human-aided dispersal and population bottlenecks facilitate parasitism escape in the most invasive mosquito species.

During biological invasion process, species encounter new environments and partially escape some ecological constraints they faced in their native range, while they face new ones. The Asian tiger mosquito Aedes albopictus is one of the most iconic invasive species introduced in every inhabited continent due to international trade. It has also been shown to be infected by a prevalent yet disregarded microbial entomoparasite Ascogregarina taiwanensis. In this study, we aimed at deciphering the factors that shape the global dynamics of A. taiwanensis infection in natural A. albopictus populations. We showed that A. albopictus populations are highly colonized by several parasite genotypes but recently introduced ones are escaping it. We further performed experiments based on the invasion process to explain such pattern. To that end, we hypothesized that (i) mosquito passive dispersal (i.e. human-aided egg transportation) may affect the parasite infectiveness, (ii) founder effects (i.e. population establishment by a small number of mosquitoes) may influence the parasite dynamics, and (iii) unparasitized mosquitoes are more prompt to found new populations through active flight dispersal. The two first hypotheses were supported as we showed that parasite infection decreases over time when dry eggs are stored and that experimental increase in mosquitoes' density improves the parasite horizontal transmission to larvae. Surprisingly, parasitized mosquitoes tend to be more active than their unparasitized relatives. Finally, this study highlights the importance of global trade as a driver of biological invasion of the most invasive arthropod vector species.

Programmable spin and transport of a living shrimp egg through photoacoustic pressure.

In the fields of biomedicine and microfluidics, the non-contact capture, manipulation, and spin of micro-particles hold great importance. In this study, we propose a programmable non-contact manipulation technique that utilizes photoacoustic effect to spin and transport living shrimp eggs. By directing a modulated pulsed laser toward a liquid-covered stainless-steel membrane, we can excite patterned Lamb waves within the membrane. These Lamb waves occur at the interface between the membrane and the liquid, enabling the manipulation of nearby particles. Experimental results demonstrate the successful capture, spin, and transport of shrimp eggs in diameter of 220 µm over a distance of about 5 mm. Calculations indicate that the acoustic radiation force and torque generated by our photoacoustic manipulation system are more than 299.5 nN and 41.0 nN·mm, respectively. The system surpasses traditional optical tweezers in terms of force and traditional acoustic tweezers in terms of flexibility. Consequently, this non-contact manipulation system significantly expands the possibilities for applications in various fields, including embryo screening, cell manipulation, and microfluidics.

Methods and Tools Used for Biosecurity Assessment in Livestock Farms in Africa: A Scoping Review

Farm biosecurity has gained increasing attention worldwide during the last decades because of its role in reducing the occurrence of diseases and improving animal performance. Recently, recommendations to reinforce the concept of farm biosecurity in lower- and middle-income countries have been advised. Therefore, this review aims to provide a comprehensive description of the methods and tools used to assess biosecurity compliance in livestock farms in Africa and formulate recommendations. The present review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analysis extension for scoping reviews guidelines. Peer-reviewed studies reporting biosecurity assessment in poultry, cattle, pig, goat, or sheep farms in Africa were included. Five databases were searched with no date restrictions. A total of 41 studies across 17 countries were finally selected. Selected studies were all published after 2008, and an increasing trend in the number of papers published per year was noticed. In total, 41 different methods for biosecurity assessment were found to be used in African countries, meaning that even within the same country, the same animal species, and the same farming system, different methods were utilized. In many papers, the methods used for biosecurity evaluation were poorly described. In addition, during the biosecurity assessment, measures related to the purchase of laying hens, egg transport and management, calves, calving and dairy management, and nursery units were almost not considered. These measures should be contemplated in future studies since they are related to important risk factors for the introduction and dissemination of infectious diseases. Interestingly, some measures not considered in European biosecurity tools were identified in the selected studies. The observed high difference in methods used may be due to the lack of regulations on biosecurity in African countries; therefore, the authors recommend the development and implementation of a harmonized and contextualized method for the assessment of biosecurity in livestock farms in Africa.

Genetic analyses verify sexually mature escaped farmed Atlantic cod and farmed cod eggs in the natural environment.

Elucidating the effects of domesticated organisms escaping into the natural environment represents a topic of importance in both evolutionary and conservation biology. However, when excluding the abundant data on salmonids, there is a lack of knowledge on this topic for marine fish aquaculture, which continues to expand globally. In order to bridge this empirical gap, we investigated a suspected escape of sexually mature domesticated Atlantic cod from a commercial marine fish farm in northern Norway. This involved genotyping samples of fish from cages on the farm, putatively identified escapees and wild cod captured in the region and samples of recently spawned eggs collected in the sea. Genetic analyses confirmed a farmed ancestry of the suspected escapees, and significantly, 27% of the sampled cod eggs. Furthermore, statistical analyses revealed a strong reduction in genetic variation in all samples of the farmed cod, including low effective population size and high degree of siblingship. These results thus document the escape of sexually mature adult cod and the release of fertilized domesticated cod eggs into the natural environment. Although it is possible that some of the mature escapees spawned post-escape, the fact that only a single egg of potential hybrid farmed × wild origin was identified, together with the high number of mature cod in the farm, points to within cage spawning as the primary source of these eggs. This suggestion is supported by oceanic particle-drift modelling, verifying that transport of eggs between the farm and the egg sampling locations was plausible. This study represents a rare documentation of interaction between domesticated and wild populations for a marine fish, pointing towards potential impacts on the local wild population.

Casting light on the European anchovy: from biology to conservation and industry

This manuscript explores the role of European anchovies (Engraulis encrasicolus) in the central Mediterranean Region, shedding light on their ecological significance, conservation challenges, and sustainable utilization. The European anchovy is one of Europe’s most important fish resources in the Mediterranean basin, and it is considered a keystone species, playing a pivotal role in both ecological and socio-economic dimensions. However, in recent decades, European anchovy, together with Sardina pilchardus (commonly known as European sardine), has suffered a population decline for several reasons. Consequently, it is necessary to improve the management of anchovy fisheries by understanding the reproductive modes and characteristics, the influence of currents on the passive transport of eggs and larvae, the feeding habits, the environmental adaptability (e.g., salinity), and the distribution of ecotypes along the Italian coasts. Such information is fundamental for the management of fisheries, especially artisanal ones, and to control frauds, especially in protected, geographically referred, and traditional high-quality commercial products. Various aspects, ranging from their population dynamics to their industrial processing and the ecological implications of these activities were delved, highlighting the knowledge about anchovy populations and ecotypes and its importance in maintaining ecosystem balance and sustaining human communities. The ecological interaction of anchovies within the food web, as essential data in the conservation actions and management of these resources was emphasized. In addition, the metabolic and stomach contents diversity among anchovy populations and ecotypes was discussed, enhancing our understanding of their adaptability to varying environmental conditions. The manuscript then explores the traditional and industrial processing of anchovies, encompassing aspects ranging from fishing techniques (i.e., methods of capture) to their industrial significance, sustainability concerns, issues of fraud, and the establishment of geographical traceability. Finally, the opportunities for sustainable and biotechnological utilization of anchovy discards were also further explored, demonstrating the potential for waste reduction and resource optimization.

ВИЗНАЧЕННЯ ОПТИМАЛЬНИХ ЦЕНТРІВ РОЗТАШУВАННЯ РАЙОНІВ ЗАБЕЗПЕЧЕННЯ ЯЙЦЯМИ ГЯНДЖІНСЬКО-ДАШКАСАНСЬКОГО ТА КАЗАХСКО-ТОВУЗСЬКОГО ЕКОНОМІЧНИХ РАЙОНІВ

The purpose of the article. Most of the products of the main demand among livestock products are perishable products. Along with the requirements attributed to the quality of this category of products, the requirements for their storage distinguish them from other products. This feature is due to the need to maintain the required temperature regime throughout the entire period of existence of perishable products, from the moment of their manufacture to the moment of consumption. Thus, the quality of the transported product is ensured, on which the safety of the population directly depends. Thus, the process of transporting perishable products is considered actual in its essence. This makes it necessary to investigate, analyze and solve existing problems in the specified area. This article is devoted to the problem of transportation of eggs to the supply points of livestock products produced in farms. The methodology and methods used are the theoretical and methodological basis of the research, the works of local and foreign scientists dedicated to the deployment of production forces, the transport provision of cargo transportation, as well as the operation of the collector transport system, scientific literature on statistics, probability, graphs and management theories, economic mathematical modeling. The main scientific innovation put forward is the development of the methodology, mathematical model of the optimal location of supply and reception points in the territory according to the minimum cost of transportation and the maximum profit, and the determination of optimal centers. The result of the research is the determination of the optimal location centers by establishing a mathematical model of the supply points of the eggs belonging to livestock products in Ganja-Dashkasan and Gazakh-Tovuz economic regions. Based on the software developed in the MathCAD computer optimization system, it is possible to determine the coordinates of the optimal location centers of the supply points, which are considered relevant as a large-scale problem in a short period of time. The high accuracy and authenticity of the obtained results indicate the possibility and significance of the wide application of the developed methodology in agriculture.

Convergent differentiation of multiciliated cells

Multiciliated cells (MCCs) are epithelial cells that control body fluid flow and contribute to the clearance of pathogenic microbes and other particles from the airways, egg transport in oviducts, and circulation of cerebrospinal fluid in the central nervous system. Although MCCs have shared functions to control fluid flow via coordinated motility of multiple ciliary structures, they are found in multiple mammalian tissues originating from distinct germ layers and differentiate via distinct developmental pathways. To understand the similarities and differences of MCCs in multiple tissues, we investigated single-cell transcriptome data of nasal epithelial cells, bronchial tubes, fallopian tubes, and ependymal cells in the subventricular zone from humans and mice by cross-species data integration. Expression of cilia-associated genes was indistinguishable between these MCCs, although cell populations had unique properties by the species and tissue, demonstrating that they share the same final differentiation status for ciliary functions. We further analyzed the final differentiation step of MCCs from their distinctive progenitors and confirmed their convergent gene set expression for ciliogenesis at the final step. These results may provide new insight into understanding ciliogenesis during the developmental process.

The influence of freshwater inflow and seascape context on occurrence of juvenile spotted seatrout Cynoscion nebulosus across a temperate estuary.

Spotted seatrout, a popular recreational sport fish in the southeastern United States, are affected by freshwater flow conditions and the availability of estuarine habitat. However, the relative influence of these factors, particularly on early life stages of seatrout, remains uncertain. We used generalized linear models to quantify relationships between the probability of encountering juvenile spotted seatrout during seine surveys and various factors, including freshwater inflow conditions, the availability and richness of estuarine habitats (seagrass, salt marsh, oyster beds) around (400-m radius) fish collection sites (seascape-scale context), as well as distance to the nearest inlet to the Gulf of Mexico (estuary-scale context) across shallow waters (< 1.5 m depth) of Apalachicola Bay, Florida. Modelling results showed a consistent positive correlation between seagrass area and the probability of encountering juvenile seatrout (all four size classes from 15mm-200mm Standard Length (SL)). The probability of encountering the two smallest juvenile seatrout size classes (15-50mm and 51-100mm SL) was also related to freshwater inflow conditions, particularly within a 3-month period prior to and including peak recruitment. Freshwater inflow may affect early life stages by influencing passive transport of eggs and larvae, planktonic food availability, and predation pressure through increases in turbidity. In contrast, encounter probabilities of the two larger size classes (101-150mm and 151-200mm) were unrelated to freshwater inflow. Inflow-related processes may be less important to the larger juveniles as they have typically settled out of the plankton into benthic habitats which provide refuge from predation and abundant benthic food sources which are not as closely tied to freshwater inflow effects. In addition, models revealed that occurrence of the larger juveniles was related to the availability of nearby habitat types such as oyster beds and salt marshes, suggesting that increased mobility as seatrout grow may allow them to use nearby habitat types as additional sources of food and refuge. These results add to a growing body of literature aimed at understanding the influence of freshwater inflow as well as seascape context on vulnerable juvenile life stages of fishery species to provide more informed strategies for freshwater inflow management and habitat conservation.

Classical Estrogen Signaling in Ciliated Epithelial Cells of the Oviduct Is Nonessential for Fertility in Female Mice.

Ciliary action performs a critical role in the oviduct (Fallopian tube) during pregnancy establishment through sperm and egg transport. The disruption of normal ciliary function in the oviduct affects oocyte pick-up and is a contributing factor to female infertility. Estrogen is an important regulator of ciliary action in the oviduct and promotes ciliogenesis in several species. Global loss of estrogen receptor α (ESR1) leads to infertility. We have previously shown that ESR1 in the oviductal epithelial cell layer is required for female fertility. Here, we assessed the role of estrogen on transcriptional regulation of ciliated epithelial cells of the oviduct using single-cell RNA-sequencing analysis. We observed minor variations in ciliated cell genes in the proximal region (isthmus and uterotubal junction) of the oviduct. However, 17β-estradiol treatment had little impact on the gene expression profile of ciliated epithelial cells. We also conditionally ablated Esr1 from ciliated epithelial cells of the oviduct (called ciliated Esr1d/d mice). Our studies showed that ciliated Esr1d/d females had fertility rates comparable to control females, did not display any disruptions in preimplantation embryo development or embryo transport to the uterus, and had comparable cilia formation to control females. However, we observed some incomplete deletion of Esr1 in the ciliated epithelial cells, especially in the ampulla region. Nevertheless, our data suggest that ESR1 expression in ciliated cells of the oviduct is dispensable for ciliogenesis and nonessential for female fertility in mice.

Secretory oviducts contribute to the high egg-laying rate of physogastric termite queens (Isoptera: Termitidae)

Physogastric termite queens are characterized by a notorious enlargement of the abdomen triggered by an equal development of the ovaries. Other physogastry-related modifications have been reported on the fat body, cuticle, midgut, tracheal system, and hemolymph. Surprisingly, modifications on the lateral oviducts of these females, important sites for ovulation and egg transport, have received little attention. Here we took advantage of the high fecundity of physogastric queens in three termitid species to evaluate ovary development and also to compare the morphophysiological features of the lateral oviducts between early-mated and physogastric queens of Cornitermes cumulans. Older queens show well-developed ovaries, with numerous ovarioles connected to the lateral oviducts through pedicels. At these sites, several corpora lutea were observed, residual follicle cells from previous ovulation events. Such features were absent among early-mated queens and reflect then the maturity and ageing of the queens. Histological and histochemical analyses indicated that secretory activity of the lateral oviducts was also restricted to physogastric queens, in which proteins, but not polysaccharides, are secreted into the oviduct lumen. The likely function of these proteins, based on previous studies, is to lubricate the lateral oviducts and stimulate muscular contractions to the egg transport. The physogastry of termite queens is a notorious feature, characterized by several body modifications, especially concerning the ovaries. Our results shed light on the physogastry-related changes in the lateral oviducts of termite queens, as their increasing secretory activity is in agreement with the high number of eggs produced and transporting through these structures. Thus, such changes correspond to an important step allowing the high egg-laying rate shown by physogastric termite queens.

Ultrastructure of the female pedal gonad in Phoxichilidium femoratum (Chelicerata, Pycnogonida)

Phoxichilidium femoratum is a common species of sea spiders – a small and unique group of chelicerates with unusual adult anatomy. In particular, substantial parts of the reproductive system in pycnogonids (unlike euchelicerates) are located in the appendages. Existing studies of pycnogonid gonads are often limited to light-microscopic level, cover a small range of species, and focus on the contents of the gonad diverticula. Ultrastructural data are rare and contradictory, and the organisation of the gonad wall and the gonoducts is unknown. Here we present a detailed light and transmission electron microscopy-based examination of the pedal portion of the adult female reproductive system in Phoxichilidium femoratum Rathke, 1799. We describe its gross anatomy and the ultrastructure of the gonad diverticulum, oviduct and gonopore, as well as development of the oocytes. Each gonad diverticulum is enclosed in the extracellular matrix of the horizontal septum and bears some internal cellular lining. However, neither the gonad lining, nor the septum sheath cells, ever form a continuous epithelial layer. Oocytes, which undergo maturation in the diverticulum, remain, until very late in the process, attached to the gonad wall though specialised stalk cells. Interestingly, stalk cells do not participate in egg envelope or yolk formation: both are synthesized endogenously in the oocytes. The oviduct is supplied with musculature, which assists in egg transport to the gonopore, whereas the gonopore itself is surrounded by specialised glands.

Simulated transport of flathead grey mullet Mugil cephalus eggs: Effects of density and duration

Flathead grey mullet has a great potential as a low trophic level aquaculture species, however captive rearing is still hampered by the lack of well-established production protocols. Here we examined the effects of transport time and egg loading density on water quality, egg survival, hatching success and early larvae performance when shipped for 8, 16 and 24 h at 500 and 1500 eggs L−1. When no water conditioners were used, survival immediately after transport was consistently over 60% up to 16 h shipment for both densities tested, with minimum values of 40% for 1500 eggs L−1. Density and transport time did not influence hatching rate (p > .05) which ranged between 31 and 54% after 8 and 16 hour transport. However, manipulation after 8 and 24 h transport led to a significant drop in early larvae survival regardless the density tested (20% and 5% respectively) compared to 16 h transport (40%) (p < .05). Hence, the results indicate that grey mullet is sensitive when manipulated as early hatched larvae and when eggs undergo blastophore closure at circa 20 h post-fertilization.The tested egg stocking densities do not deteriorate water quality to lethal levels in terms of pH, NO2 and NO3 especially for short and medium transportation times, however, 24 h transportation induces an accumulation of ammonia affecting the long-term survival of larvae. When basic technology is available (i.e. no water conditioners, refrigerated vehicles), we suggest manipulating grey mullet eggs at 28 h post fertilization for best results in terms of hatching rate and early larvae survival.

Optimization of stocking density and shipping duration for transportation of Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel), eggs, and experimental verification of polyethylene glycol treatment to reduce collision damage

AbstractPacific bluefin tuna (PBT), Thunnus orientalis, is one of the most important species for aquaculture worldwide. For the conservation of this species and sustainable development of the tuna farming industry, production based on closed‐cycle aquaculture should be promoted. In this study, to develop an efficient method for transporting PBT eggs, we simulated egg transportation to optimize stocking density and shipping duration, which are the two variables critical to the transportation procedure of PBT. Based on the findings of this study, we concluded that PBT eggs should be transported at a density of 1 × 104 eggs/L within 12 h in the field. Furthermore, we showed that collisions between eggs during transportation negatively affected the normal hatching rates of eggs, and polyethylene glycol (PEG) treatment (at 1000 μg/mL for 12 h) could reduce such damage. Our results suggest that PEG treatment is a practical approach that contributes to the stable transportation of PBT eggs, particularly in cases where the effects of physical shock damage is considerably increased.

Reassortant viruses threatening fish aquaculture

AbstractAquaculture provides more than half of fish destined for human consumption worldwide. In aquaculture, infectious diseases triggered by viruses are amongst the major cause of mortality of farmed fish. The cohabitation of different virus strains in the same geographical area opens the possibility to natural reassortment. Virus reassortment is a characteristic recombination process of segmented viruses in which different viruses merge their genome by the shuffling of complete segments. This is possible when two or more viral strains are cohabitating into a single host, boosting their probability to concatenate their viral cycles, getting to pack segments from distinct parental strains. Cohabitation of different viruses in a geographical area, and therefore the possibility of natural reassortment, has been aided by the increment in the transport of eggs and larvae between facilities around the world. Reassortant viruses have been described to show special tropisms for fish species opposed from those affected by parental virus strains, entailing an emerging threat for aquaculture but also for wild populations. To date, outbreaks of reassortant strains of infectious pancreatic necrosis virus, nodavirus (NNV), infectious salmon anaemia virus and tilapia lake virus have been described. Unfortunately, only the characterization of reassortant NNV has been explored. The aim of this review is compiling the information available concerning reassortant viruses affecting fish species of relevance for aquaculture highlighting their potential impact for the sector. Additionally, recent research advances in host‐NNV interaction are also included.

Sperm utilize tumor necrosis factor alpha to shut down a 2-methoxyestradiol nongenomic pathway that accelerates oviductal egg transport in the rat.

Mating shuts down the 2-methoxyestradiol (2ME) nongenomic pathway that accelerates oviductal egg transport in the rat. This study shows that sperm cells, but not vaginocervical stimulation, utilize TNF-α to shut down this 2ME nongenomic pathway. The transport of oocytes or embryos throughout the oviduct to the implantation site in the uterus is defined as egg transport. In the rat, 2-methoxyestradiol (2ME) accelerates egg transport through the oviduct via a nongenomic pathway. Mating is known to shut down this 2ME pathway and then trigger an estradiol genomic pathway that accelerates egg transport. Here, we tested whether intrauterine insemination (IUI) or vaginocervical stimulation (VCS) shuts down the 2ME nongenomic pathway that accelerates egg transport, and if these mating components require tumor necrosis factor alpha (TNF-α). Levels of TNF-α and the mRNA for TNF-α receptors were measured in the oviduct of IUI or VCS rats. The tissue distribution of TNF-α receptor proteins and the concentration of the mRNA for catechol-O-methyl transferase (Comt) and 2ME were also analyzed in the oviduct. Finally, we assessed whether 2ME accelerates egg transport in IUI or VCS rats previously treated with the TNF-α antagonist W9P9QY. Results show that IUI, but not VCS, increased TNF-α and their receptors in the oviduct. IUI and VCS did not change the tissue distribution of TNF-α receptors; however, both decreased the oviductal concentration of Comt and 2ME. IUI and VCS each blocked the 2ME-induced egg transport acceleration; however, only the IUI was antagonized by the TNF-α antagonist. We concluded that IUI and VCS inhibit the 2ME nongenomic pathway that accelerates egg transport; however, the vias of action are distinct, with a TNF-α increase on spermatozoa presence being required for the shutdown of the 2ME pathway.