Spam reviews pose a significant challenge to the integrity of online platforms, misleading consumers and undermining the credibility of genuine feedback. This paper introduces an innovative AI-generated spam review detection framework that leverages Deep Learning algorithms and Natural Language Processing (NLP) techniques to identify and mitigate spam reviews effectively. Our framework utilizes multiple Deep Learning models, including Convolutional Neural Networks (CNNs), Long Short-Term Memory (LSTM) networks, Gated Recurrent Unit (GRU), and Bidirectional LSTM (BiLSTM), to capture intricate patterns in textual data. The system processes and analyzes large volumes of review content to detect deceptive patterns by utilizing advanced NLP and text embedding techniques such as One-Hot Encoding, Word2Vec, and Term Frequency-Inverse Document Frequency (TF-IDF). By combining three embedding techniques with four Deep Learning algorithms, a total of twelve exhaustive experiments were conducted to detect AI-generated spam reviews. The experimental results demonstrate that our approach outperforms the traditional machine learning models, offering a robust solution for ensuring the authenticity of online reviews. Among the models evaluated, those employing Word2Vec embeddings, particularly the BiLSTM_Word2Vec model, exhibited the strongest performance. The BiLSTM model with Word2Vec achieved the highest performance, with an exceptional accuracy of 98.46%, a precision of 0.98, a recall of 0.97, and an F1-score of 0.98, reflecting a near-perfect balance between precision and recall. Its high F2-score (0.9810) and F0.5-score (0.9857) further highlight its effectiveness in accurately detecting AI-generated spam while minimizing false positives, making it the most reliable option for this task. Similarly, the Word2Vec-based LSTM model also performed exceptionally well, with an accuracy of 97.58%, a precision of 0.97, a recall of 0.96, and an F1-score of 0.97. The CNN model with Word2Vec similarly delivered strong results, achieving an accuracy of 97.61%, a precision of 0.97, a recall of 0.96, and an F1-score of 0.97. This study is unique in its focus on detecting spam reviews specifically generated by AI-based tools rather than solely detecting spam reviews or AI-generated text. This research contributes to the field of spam detection by offering a scalable, efficient, and accurate framework that can be integrated into various online platforms, enhancing user trust and the decision-making processes.