Efficiency Of Microbial Protein Synthesis Research Articles

Evaluation Additional of Synbiotic Powder in Feed to Improve Milk Production and Quality of Dairy Cattle

Evaluation Additional of Synbiotic Powder in Feed to Improve Milk Production and Quality of Dairy Cattle

The balance of rumen degradable protein and readily available carbohydrate in sheep rations on in vitro fermentability.

Protein and carbohydrates are substances needed by ruminants, especially sheep. Providing protein and carbohydrates must pay attention to their degradation. In addition, balancing nutrients to meet the nutritional needs of rumen microbes is very important because the unbalanced availability of rumen degradable protein (RDP) and readily available carbohydrate (RAC) at one time can cause suboptimal microbial protein synthesis efficiency. Completely randomized design with a nonfactorial pattern of five treatments with three replications. Treatment includes RDP and RAC ratios, namely R1 = 2.30, R2 = 2.00, R3 = 1.70, R4 = 1.50, and R5 = 1.30. The results showed that the balance of RDP and RAC in sheep ration formulation in vitro had a very significant effect (p < 0.01) on NH3, microbial protein synthesis, total gas, total microbes, and organic matter digestibility (OMD) but had no significant effect (p > 0.05) on rumen pH and dry matter digestibility (DMD). The optimal balance of RDP and RAC in the formulation of sheep rations in vitro was obtained at a ratio of 2.30 with NH3 (mM) 8.47, rumen pH 5.97, microbial protein synthesis (mg/100 ml) 123, gas (ml/g of material) 145, total microbes (cells/ml) 2.012 × 106, (log CFU cells/ml) 6.3025, DMD 61.0%, and OMD 63.1%.

Effect of pregnancy and feeding level on voluntary intake, digestion, and microbial nitrogen synthesis in Zebu beef cows.

The objective of this research was to evaluate how pregnancy and feeding regimens affect the feed intake, digestibility, and efficiency of microbial nitrogen (N) synthesis in beef cows. Forty-four multiparous Nellore cows, comprising 32 gestating and 12 non-gestating cows, with an average weight of 451 ± 10kg, were assigned to either a HIGH (ad libitum) or LOW (limited feeding at 1.2 times maintenance based on the NRC) feeding regimen during the gestational period. The dry matter intake (DMI) in kg/d was significantly greater (P < 0.01) in HIGH-fed cows. The DMI reduced (P < 0.05) in proportion to the shrunk body weight (SBW) as days of pregnancy (DOP) increased. The interaction between feeding level and DOP was significant (P < 0.05) for the digestibility of dry matter (DM), organic matter (OM), N compounds, ether extract (EE), ash- and protein-free neutral detergent fiber (NDFap), gross energy (GE), and total digestible nutrients (TDN). Except for DM and TDN digestibility, there was a reduced nutrient digestibility as gestation progressed in HIGH-fed cows. In contrast, digestibility increased as a function of DOP in LOW-fed cows. Microbial N synthesis (g/day) was significantly higher in HIGH-fed cows (P < 0.001) compared to LOW-fed cows. The efficiency of microbial N production per g of N intake and kg of digestible OM intake was (P = 0.021) and tended (P = 0.051) to be greater in LOW-fed cows compared to HIGH-fed cows. In summary, HIGH-fed Nellore cows reduce feed intake and digestibility with advancing gestation, affecting feed utilization. In addition, LOW-fed cows, showed higher microbial protein synthesis efficiency, potentially making them more nutrient-efficient under challenging nutritional conditions.

Assessment of Nutritional Potential and Methane Mitigation Efficacy of Napier Grass Cultivars

Napier grass is cultivated as a high-yielding forage crop and widely utilized in the nutrition of grazing animals such as cattle and goats due to its rapid growth rate, expansive leaf surface, and ability to thrive even in low-quality soils, making it a valuable animal feed option. In this study, the chemical compositions, in vitro gas (GP) and methane (CH4) production, metabolizable energy (ME), organic matter digestibility (OMD), true digestible dry matter (TDDM), microbial protein (MP), partitioning factor (PF), microbial protein synthesis efficiency (MPSE, and relative feed values (RFV) of the grasses Packhong1 (Penisetum purpureum x Penisetum americanum), Chinese Pennisetum (Penisetum purpureum x Penisetum alopecurides), and Dwarf Elephant (Pennisetum purpureum cv. Mott) were investigated. Significant differences were found in the chemical contents of napier varieties used in the study (p

Dose response to postruminal urea in lactating dairy cattle

Inclusion of urea in dairy cattle diets is often limited by negative effects of high levels of feed urea on dry matter intake (DMI) and efficiency of rumen N utilization. We hypothesized that supplying urea postruminally would mitigate these limitations and allow greater inclusion of urea in dairy cattle diets. Four rumen-fistulated Holstein-Friesian dairy cows (7 ± 2.1 lactations, 110 ± 30.8 d in milk; mean ± standard deviation) were randomly assigned to a 4 × 4 Latin square design to examine DMI, milk production and composition, digestibility, rumen fermentation, N balance, and plasma constituents in response to 4 levels of urea continuously infused into the abomasum (0, 163, 325, and 488 g/d). Urea doses were targeted to linearly increase the crude protein (CP) content of total DMI (diet plus infusion) by 0%, 2%, 4%, and 6% and equated to 0%, 0.7%, 1.4%, and 2.1% of expected DMI, respectively. Each 28-d infusion period consisted of a 7-d dose step-up period, 14 d of adaptation, and a 7-d measurement period. The diet was fed ad libitum as a total mixed ration [10.9% CP, 42.5% corn silage, 3.5% grass hay, 3.5% wheat straw, and 50.5% concentrate (dry matter basis)] and was formulated to meet 100%, 82%, and 53% of net energy, metabolizable protein, and rumen-degradable protein requirements, respectively. Linear, quadratic, and cubic effects of urea dose were assessed using polynomial regression assuming the fixed effect of treatment and random effects of period and cow. Dry matter intake and energy-corrected milk yield responded quadratically to urea dose, and milk urea content increased linearly with increasing urea dose. Apparent total-tract digestibility of CP increased linearly with increasing urea dose and ruminal NH3-N concentration responded quadratically to urea dose. Mean total VFA concentration was not affected by urea dose. The proportion of N intake excreted in feces decreased linearly and that excreted in urine increased linearly in response to increasing urea dose. The proportion of N intake excreted in milk increased linearly with increasing urea dose. Urinary urea excretion increased linearly with increasing urea dose. Microbial N flow responded cubically to urea dose, but the efficiency of microbial protein synthesis was not affected. Plasma urea concentration increased linearly with increasing urea dose. Regression analysis estimated that when supplemented on top of a low-CP diet, 179 g/d of postruminal urea would maximize DMI at 23.4 kg/d, corresponding to a dietary urea inclusion level of 0.8% of DMI, which is in line with the current recommendations for urea inclusion in dairy cattle diets. Overall, these results indicate that postruminal delivery of urea does not mitigate DMI depression as urea dose increases.

Supplementing branched-chain volatile fatty acids in dual-flow cultures varying in dietary forage and corn oil concentrations. II: Biohydrogenation and incorporation into bacterial lipids

To maintain membrane homeostasis, ruminal bacteria synthesize branched-chain fatty acids (BCFA) or their derivatives (vinyl ethers) that are recovered during methylation procedures as branched-chain aldehydes (BCALD). Many strains of cellulolytic bacteria require 1 or more branched-chain volatile fatty acid (BCVFA). Therefore, the objective of this study was to investigate BCVFA incorporation into bacterial lipids under different dietary conditions. The study was an incomplete block design with 8 continuous culture fermenters used in 4 periods with treatments (n = 4) arranged as a 2 × 2 × 2 factorial. The factors were high (HF) or low forage (LF, 67 or 33% forage, 33:67 alfalfa:orchardgrass), without or with supplemental corn oil (CO; 3% dry matter, 1.5% linoleic fatty acid), and without or with 2.15 mmol/d (5 mg/d 13C each of isovalerate, isobutyrate, and 2-methylbutyrate). After methylation of bacterial pellets collected from each fermenter's effluent, fatty acids and fatty aldehydes were separated before analysis by gas chromatography and isotope ratio mass spectrometry. Supplementation of BCVFA did not influence biohydrogenation extent. Label was only recovered in branched-chain lipids. Lower forage inclusion decreased BCFA in bacterial fatty acid profile from 9.45% with HF to 7.06% with LF and decreased BCALD in bacterial aldehyde profile from 55.4% with HF to 51.4% with LF. Supplemental CO tended to decrease iso even-chain BCFA and decreased iso even-chain BCALD in their bacterial lipid profiles. The main 18:1 isomer was cis-9 18:1, which increased (P < 0.01) by 25% from CO (data not shown). Dose recovery in bacterial lipids was 43.3% lower with LF than HF. Supplemental CO decreased recovery in the HF diet but increased recovery with LF (diet × CO interaction). Recovery from anteiso odd-chain BCFA and BCALD was the greatest; therefore, 2-methylbutyrate was the BCVFA primer most used for branched-chain lipid synthesis. Recovery in iso odd-chain fatty acids (isovalerate as primer) was greater than label recovery in iso even-chain fatty acids (isobutyrate as primer). Fatty aldehydes were less than 6% of total bacterial lipids, but 26.0% of 13C recovered in lipids were recovered in BCALD because greater than 50% of aldehydes were branched-chain. Because BCFA and BCALD are important in the function and growth of bacteria, especially cellulolytics, BCVFA supplementation can support the rumen microbial consortium, increasing fiber degradation and efficiency of microbial protein synthesis.

Supplementing branched-chain volatile fatty acids in dual-flow cultures varying in dietary forage and corn oil concentrations. I: Digestibility, microbial protein, and prokaryotic community structure

Branched-chain amino acids are deaminated by amylolytic bacteria to branched-chain volatile fatty acids (BCVFA), which are growth factors for cellulolytic bacteria. Our objective was to determine the dietary conditions that would increase the uptake of BCVFA by rumen bacteria. We hypothesized that increased forage would increase cellulolytic bacterial abundance and incorporation of BCVFA into their structure. Supplemental polyunsaturated fatty acids, supplied via corn oil (CO), should inhibit cellulolytic bacteria growth, but we hypothesized that additional BCVFA would alleviate that inhibition. Further, supplemental BCVFA should increase neutral detergent fiber degradation and efficiency of bacterial protein synthesis more with the high forage and low polyunsaturated fatty acid dietary combination. The study was an incomplete block design with 8 dual-flow continuous cultures used in 4 periods with 8 treatments (n = 4 per treatment) arranged as a 2 × 2 × 2 factorial. The factors were: high forage (HF) or low forage (LF; 67 or 33%), without or with supplemental CO (3% dry matter), and without or with 2.15 mmol/d (which included 5 mg/d of 13C each of BCVFA isovalerate, isobutyrate, and 2-methylbutyrate). The isonitrogenous diets consisted of 33:67 alfalfa:orchardgrass pellet, and was replaced with a concentrate pellet that mainly consisted of ground corn, soybean meal, and soybean hulls for the LF diet. The main effect of supplementing BCVFA increased neutral detergent fiber (NDF) degradability by 7.6%, and CO increased NDF degradability only in LF diets. Supplemental BCVFA increased bacterial N by 1.5 g/kg organic matter truly degraded (6.6%) and 0.05 g/g truly degraded N (6.5%). The relative sequence abundance decreased with LF for Fibrobacter succinogenes, Ruminococcus flavefaciens, and genus Butyrivibrio compared with HF. Recovery of the total 13C dose in bacterial pellets decreased from 144 µg/ mg with HF to 98.9 µg/ mg with LF. Although isotope recovery in bacteria was greater with HF, BCVFA supplementation increased NDF degradability and efficiency of microbial protein synthesis under all dietary conditions. Therefore, supplemental BCVFA has potential to improve feed efficiency in dairy cows even with dietary conditions that might otherwise inhibit cellulolytic bacteria.

Fermentation parameters in the rumen of goats supplemented with polyphenol oxidase derived from Gliricidia sepium leaves under in vitro conditions

Abstract. Huda AN, Sabarudin A, Jayanegara A, Soetanto H. 2023. Fermentation parameters in the rumen of goats supplemented with polyphenol oxidase derived from Gliricidia sepium leaves under in vitro conditions. Biodiversitas 24: 3282-3290. The growing demand for healthy foods derived from ruminant products is restricted by the high content of saturated fatty acids in meat or milk due to the process of rumen biohydrogenation. Recent reports from intensive studies using polyphenol oxidase (PPO), an enzyme in plants’ secondary metabolism, as the inhibitor of rumen biohydrogenation cost doubt of ruminant products. PPO is assumed to increase the supply of UFA by reducing the rate of biohydrogenation due to rumen microbes’ activity. Microbes of the rumen, including species of bacteria and protozoa are responsible for rumen biohydrogenation. The present study investigated the effect of adding PPO derived from Gliricidia sepium leaves on fermentation parameters in the rumen, including protozoa density and diversity, fatty acid profile, volatile fatty acid profile, and the efficiency of microbial protein synthesis under in vitro conditions. The experiment was carried out through an in vitro rumen fermentation method, consisting of five treatments, i.e., negative control, positive control, the addition of 0.1 ml, 0.3 ml, and 0.5 ml PPO emulsion. The most dominant type of protozoa among all treatments was Entodinium spp. PPO emulsion increases the efficiency of microbial protein synthesis and volatile fatty acid concentration. 0.5 ml PPO emulsion increases linoleic acid. In conclusion, this finding poses a question on the role of rumen protozoa in lipid biohydrogenation in the rumen. A further experiment on the benefits of adding PPO regarding rumen biohydrogenation under in vivo conditions is warranted.

Effects of the addition of non-fibre carbohydrates with different rumen degradation rates in dairy cow high-forage diets using the Rumen Simulation Technique.

Nutrient synchronisation of protein and carbohydrates is a promising practice to improve ruminal nutrient utilisation. However, dietary sources supplying these nutrients can vary in ruminal nutrient availability due to differing degradation rates, therefore potentially affecting utilisation of nitrogen (N). The effects of the addition of non-fibre carbohydrates (NFCs) with different rumen degradation rates in high-forage diets on ruminal fermentation, efficiency and microbial flow were investigated in vitro using the Rumen Simulation Technique (RUSITEC). Four diets were tested: control with 100% ryegrass silage (GRS) and substitution of 20% on a DM basis of ryegrass silage with corn grain (CORN), processed corn (OZ) or sucrose (SUC). The four diets were assigned to 16 vessels in two sets of RUSITEC apparatuses in a randomised block design over a 17 d experimental trial; 10 d consisted of adaptation and 7 d for sample collection. Rumen fluid was collected from four rumen-cannulated dry Holstein-Friesian dairy cows and was treated without mixing. Then, rumen fluid from each cow was used to inoculate four vessels, and diet treatments were randomly allocated to each one. This was repeated for all cows resulting in 16 vessels. The inclusion of SUC in ryegrass silage diets improved DM and organic matter digestibility. The only diet to significantly lower ammonia-N concentration compared with GRS was SUC. The outflows of non-ammonia-N, microbial-N, and efficiency of microbial protein synthesis were not affected by diet type. However, the efficiency of nitrogen utilisation was improved by SUC compared with GRS. This indicates that the inclusion of an energy source with a high rumen degradation rate in high-forage diets improves rumen fermentation, digestibility, and N utilisation. Specifically, this effect was observed for the more readily available energy source, SUC, compared with the more slowly degradable NFC sources, CORN and OZ.

Roles of tree and shrub legume leaves as protein sources for cattle raised by small farmers in Bangkalan Regency

Inclusion of tree or shrub legume leaves in the ration of beef cattle improved quality and the main indicators are the increase in crude protein content and digestibility. The present study was done from March to September 2023 involving 30 farmers in Bangkalan regency who raised madura cattle and the information gathered were type and number of feeds offered daily. Tree and shrub legume leaves such as Gliricidia, Moringa, Indigofera, and jackfruit are the most common protein sources used to supplement field grass or straws. Samples of forage were taken and the following rations were formulated to simulate cattle feed: A (grass only); B (60% grass + 40% gliricidia); C (60% grass + 40% moringa); D (60% grass + 40% Indigofera); E (60% grass + 40% jackfruit) leaves. Parameters measured using an in vitro gas production technique showed that supplementation with tree and shrub legume leaves on field grass basal diet improved dry matter digestibility (DMD) and organic matter (OMD) values and this was closely related to improvements of rumen NH3 concentration which also contributed to efficiency of microbial protein synthesis (ESPM) value improvement. It can be concluded that tree and shrub legume leaves have shown important roles in improving the quality of field grass based diet for Madura cattle.

Nitrogen balance and microbial protein synthesis in dairy cows fed with peanut meal to replace soybean meal.

This research was carried out to evaluate the effect of replacing dietary soybean meal with peanut meal, on intake and digestibility, microbial protein synthesis, and nitrogen balance in lactating cows. Twelve crossbred Holstein × Gyr cows at 84days in milk (DIM) were distributed in a triple 4 × 4 Latin square. The treatments consisted of increasing peanut meal levels (0, 330, 670, and 1000g/kg dry matter basis) in replacement of soybean meal in the diet. Replacing soybean meal with peanut meal had no effect on the intake and digestibility of dry matter and nutrients. Nitrogen balance and percentage of retained were similar among treatments. Microbial nitrogen synthesis and microbial protein synthesis efficiency were similar among treatments. Thus, in regions where its cost is lower, peanut meal can totally replace soybean meal in dairy cow diets without affecting nitrogen balance and microbial protein synthesis.

Blend of secondary metabolites from mesquite to improve nutrient digestibility, microbial protein, efficient use of nitrogen, ruminal parameters, and blood metabolites in sheep.

Although chemical additives are able to improve the efficiency of ruminal fermentation, they can leave residues in the meat. However, a blend of secondary metabolites can improve ruminal fermentation without harming the population welfare. Five levels (0.0, 1.5, 3.0, 4.5, and 6.0g/day) of a blend of secondary metabolites from mesquite extract in sheep feed to promote increases in the nutritional value, ruminal parameters, nitrogen (N) use efficiency, microbial protein (MP) synthesis, and blood metabolites. Ten intact male Santa Inês sheep with average body weight of 55 ± 9.81kg were used in a 5 × 5 Latin square design, replicated twice. There was a quadratic response of the digestibility of dry matter (DM), organic matter (OM), crude protein (CP), and total digestible nutrients (TDN). Microbial protein concentrations, MP synthesis efficiency, propionic acid levels, and acetic/propionic acid ratio also showed a quadratic response. The blend promoted a quadratic effect on plasma glucose and lactate levels. On the other hand, it decreased the concentrations of ammoniacal nitrogen, plasma urea, and plasma cholesterol. It is recommended to supply a blend of secondary metabolites at 3.43g/day.

Dose-response effects of the Savory (Satureja khuzistanica) essential oil and extract on rumen fermentation characteristics, microbial protein synthesis and methane production in vitro

Abstract The objective of the present study was to investigate dose-response effects of the essential oil (EO) and dry extract (EX) of Satureja khuzistanica (SK) on in vitro gas production kinetics, rumen fermentation, ruminal methanogenesis and microbial protein synthesis. So, EO and EX were tested at 0 (as control); 150 (low dose); 300, 450 (intermediate doses) and 600 mg/L (high dose). The gas produced over 24 h of incubation (GP24) decreased linearly with both EO and EX dosages (P&lt;0.01). In vitro methane production was reduced by both EO (14–69%, depending on the included dose) and EX (7–58%). Microbial protein (MP) as well as the efficiency of microbial protein synthesis (EMPS) were improved by EO (18.8–49.8% and 20.4–61.5% for MP and EMPS, respectively) and to a lesser extent by EX (8.3–25.7% and 4.6–24.2% for MP and EMPS, respectively). Ammonia concentration was dropped in linear and quadratic manners with EO (P&lt;0.05), and linearly with EX dosages (P&lt;0.01). EO and EX exhibited depressive effects (in linear and quadratic (P&lt;0.05), and linear manners (P&lt;0.01), respectively) on total protozoa count. A mixed linear and quadratic effect was observed from both EO and EX on total VFA concentration (P&lt;0.01). Total VFA concentration increased at 300 mg/L of EX, but decreased at high dose of both EO and EX. The acetate proportion increased with EO intermediate and high dosages, but it decreased at the expense of propionate at low and intermediate doses of EX. In total, these findings confirmed previous research on the great capacity of plant-based feed additives in positively modulating rumen fermentation that their effects may vary depending on the doses used. Specifically, these results suggest that EO and EX have high potentials to improve rumen functions at intermediate doses, which needs to be confirmed by in vivo experiments.

Nitrogen utilization and nutrient intake and digestibility can be improved in sheep fed cotton-burrs with crude glycerin

The present study evaluated the replacement of corn grain with increasing levels of crude glycerin (CG) in cotton burrs-based diets for sheep in terms of intake and digestibility, nitrogen (N) metabolism, microbial protein synthesis (MPS) efficiency, blood glucose, and ruminal parameters. Five male sheep (body weight 52.2 ± 5.5 kg), castrated and rumen-fistulated, were used in a randomized 5 × 5 Latin square design. The experimental period consisted of 17 d (10 d for adaptation and 7 d for sampling). Isonitrogenous diets with 112 g crude protein (CP) per kg dry matter (DM) were formulated to contain 0 (control), 50, 100, 150, and 200 g CG/kg DM. Compared to the control diet, feeding CG up to 200 g/kg DM quadratically changed (P < 0.01) nutrient intake. Maximum intake of DM, organic matter (OM), neutral detergent fiber (aNDF), and water was achieved when feeding 150 g CG/kg DM. Compared to zero CG inclusion, feeding 200 g CG/kg DM linearly increased (P ≤ 0.03) the digestibility of OM and aNDF by 3.4 % and 13.6 %, respectively, while DM digestibility tended to increase linearly by 6 % (P = 0.05). The apparent N digestibility increased linearly (P < 0.02) by 6.8 % and 10.4 % with GC inclusion levels of 150 and 200 g/kg DM, respectively. Nitrogen intake, fecal and urinary N, absorbed N, and N absorbed per unit of N intake were all quadratically changed (P ≤ 0.03) by CG inclusion. These parameters increased by 34.7 %, 15.7 %, 34.5 %, 49.3 %, and 10.7 %, respectively, when a diet of 150 g CG/kg DM was fed. Feeding different levels of CG in a sheep diet did not affect (P ≥ 0.12) ruminal MPS (g microbial N/d) and MPS efficiency (g microbial CP/kg total digestible nutrients), ruminal pH, and concentrations of ruminal ammoniacal-N and blood glucose. Crude glycerin included up to 150 g/kg in cotton burrs-based diets for sheep improved N utilization and intake and digestibility of nutrients without affecting ruminal parameters, glucose blood concentrations, and MPS efficiency.

Effect of vitamin blend supplementation on the ingestive, digestive, and ruminal parameters of Nellore cattle

Rumen cannulated Nellore bulls (n = 4) were used in a 4 × 4 Latin square design, with a 2 × 2 factorial scheme to evaluate the effects of vitamin supplementation on: (1) ingestive, digestive, and ruminal parameters; (2) serum concentrations of 25-hydroxyvitamin D (25(OH)D); and (3) in situ degradability of complete diets. The factors consisted of two fat-soluble vitamin blend (ADE) supplementation levels (ADE− or ADE+) and two B vitamin blend (biotin, niacin, and thiamine) supplementation levels (B-blend− or B-blend+). Thus, the treatments evaluated consisted of no vitamin supplementation (ADE− B-blend−), supplementation of a B vitamin blend (ADE− B-blend+), supplementation with a fat-soluble vitamin blend (ADE+ B-blend−), or a combination of these two blends (ADE+ B-blend+). Nutrient intake and DM, as well as ruminal digestibility were not affected (P > 0.05) by any of the vitamin supplementations. The apparent total-tract digestibility of DM, organic matter (OM), neutral detergent fiber corrected for residual ash and protein (apNDF), crude protein (CP), and non-fiber carbohydrates (NFC) were not influenced by the experimental diets (P > 0.05). Furthermore, an increase in starch total-tract digestibility was verified with ADE supplementation in the diets (P = 0.031). None of the in situ parameters were affected (P > 0.05) by vitamin supplementation. Further, the treatments did not affect ruminal kinetics (ki, kp, and kd,) of DM, OM, and starch (P > 0.05). The omasal flow of total N, NH3-N, non-ammonia N, microbial N, and non-ammonia non-microbial N (P > 0.05) or the efficiency of microbial protein synthesis (P > 0.05) were not affected by vitamin blend supplementation. The total fat volatile acid (VFA) concentrations, as well lactate concentration were not affected (P > 0.05) by vitamin blend supplementation. Bulls that consumed the combination of ADE and B-blend supplementation had lower ruminal NH3-N concentrations (P = 0.008). B-blend vitamin supplementation decreased ruminal pH (P = 0.003). As expected, concentrations of serum 25(OH)D increased (P = 0.001) in Nellore bulls fed diets with the combination of fat-soluble vitamin (ADE) and B-blend supplementation. Supplementing Nellore bulls with a vitamin blend containing 25(OH)D3 was a successful strategy for increasing circulating concentrations of 25(OH)D. The B vitamin blend (biotin, niacin, and thiamine) supplementation did not improve ruminal fermentation, ingestive or digestive parameters. However, the fat-soluble vitamin blend (ADE) supplementation increased total-tract starch digestibility.

Determining The Feed Value, Feed Quality, and in Vitro Fermentation Properties of Italian Ryegrass (Lolium multiflorum L.) Harvested in Different Periods

The purpose of this study was to determine the chemical content, in vitro gas and methane production, relative feed value (RFV), relative feed quality (RFQ), in vitro digestibility parameters, and microbial protein production of the Italian ryegrass plants harvested in different periods of the 2020 production season in Erzincan. In this study an ANKOM Daisy Incubator was used to determine the in vitro digestibility parameters. In vitro gas production technique was used todetermine gas production and predicted parameters. It was found that there were significant differences between the Italian ryegrass plants harvested in different periods in terms of composition, RFV, RFQ, and in vitro digestibility and fermentation parameters. The RFV, metabolizable energy (ME), net energy (NE), true NDF digestibility (TNDFD), organic matter digestibility degree (OMDD), total digestible nutrient (TDN), dry matter intake (DMI), and RFQ values of the Italian ryegrass plants were found to be 247.32, 9.13, 5.68, 62.26%, 54.15%, 55.35%, 4.82%, and 215.81, respectively. On the other hand, after 24 hours of incubation it’s in vitro gas (GP), and methane production (ml and %), true dry matter digestibility (TDMD) values, partitioning factor (PF), microbial protein (MP), microbial protein synthesis efficiency (MPSE), and true digestibility (TD) were found to be 105.41, 17.35, 16.42, 281.72, 2.68, 57.68, 20.32, and 59.82, respectively. In conclusion, determining the digestibility of plants via the measurement of RFV, RFQ, digestion parameters, and gas production in different harvest periods provided insights into the potential of Italian ryegrass as a feed material.

Inclusion of Different Level Leucaena in Dairy Ration to Balance Rumen Degradable and Undegradable Protein Ratio

Abstract Sustainability use of expensive protein source in a dairy ration requires ration formulation based on rumen degradable and undegradable protein ratio (RDUP). It improves the efficiency of microbial protein synthesis and less feed residue to the environment. Providing less expensive green protein sources such as leucaena (Leucaena leucocephala) is also an alternative. This study aimed to evaluate the effect of dairy ration based on RDUP with leucaena addition on in vitro fermentability and digestibility. This study was conducted in a 4 x 3 factorial randomized block design. Factor 1 was RDUP levels (R1= 60:40; R2= 55:45; R3= 50:50; R4= 45:55) and factor 2 was Leucaena addition (L1= 10%; L2= 20%; L3= 30%). The data were analyzed using ANOVA and followed by the Duncan test. The result showed that increasing RDUP levels increased NH3 concentration and dry matter digestibility. Treatment L2 resulted in a high NH3 concentration compared to other L treatments. The combination of R1 with L2 and L3 resulted in the highest total VFA concentration than other treatments. Protozoa population increased with increasing leucaena in the ration. It is concluded that dairy ration with 60:40 rumen degradable protein to undegradable protein ratio and 20% leucaena can improve ruminal fermentation and digestibility.

Dose response relationships between linseed or rapeseed oils supply and rumen microbial metabolism in continuous culture on maize silage-based diet

Little quantitative information is available on how dietary lipids concurrently alter the main rumen microbial functions in relation with their incorporation level. In a three-period experiment, linseed (Linum usitatissimum, LO) and rapeseed (Brassica napus L., RO) oils were added at 0, 40 or 80 g/kg dry matter input (DMI) to five 1-L dual outflow fermenters that were on a maize silage-based diet for nine days per period. RO supply decreased butyrate specific production. The amount of hexoses fermented (HF) increased by 9% at 40 g/kg LO. The production of CH4 was lower at 80 g/kg LO by 46% compared with controls. Conversely, the supply of LO significantly increased H2 and H2S productions in an antagonistic mode. The specific productions of propionate, butyrate, CH4 and H2 were altered by LO. The supply of RO increased the ammonia daily outflow (by 23% at 40 g/kg) and decreased the organic N outflow (by 13% at 80 g/kg). The degradabilities of dietary fractions were not affected by RO, neither the OM partitioning between dietary, fermented and microbial outflows. The OM true degradability decreased at 80 g/kg LO compared with controls and 40 g/kg LO. When LO was supplied, isovalerate and ammonia-N outflows were higher, organic and microbial N outflows and EMPS were lower along with changes in the OM outflow partitioning. Overall microbial processes appeared to differ in their responses to fatty acids saturation. Moreover, most effects were present at 40 g/kg DMI and diminished or even plateaued at 80 g/kg DMI. Highlights Four per cent oil lowered butyrate specific production Four per cent Linseed oil lowered microbial protein synthesis efficiency Linseed oil lowered methanogenesis in proportion to input level

Effect of Grape Marc Added Diet on Live Weight Gain, Blood Parameters, Nitrogen Excretion, and Behaviour of Sheep.

Simple SummaryThis experiment explored how feeding grape wine production waste product grape marc impacts on sheep production. Forty merino sheep were divided into two groups; one group received a sheep industry standard diet (control), and one group received a treatment diet which had 20% of the control diet replaced by grape marc. The results showed that the grape marc diet led to a higher intake and faecal nitrogen/urinary nitrogen ratio, but no difference in sheep live weight gain, behaviour, and parasitic egg count compared with control diet-fed sheep. Overall, the results showed that feeding grape wine production waste product grape marc as a cheap feed, to replace 20% of the control ration, can maintain sheep productivity, health, and environmental sustainability.A 39-day field experiment was conducted to assess the effect of grape marc (GM) feeding on sheep productivity, health, and environmental sustainability. Forty merino sheep were divided into two dietary groups, each having five replications (n = 4 sheep/replication). Experimental diet consisted of: (i) control: 55% lucerne hay + 40% wheat grain + 5% faba bean; (ii) GM treatment: control diet with 20% replaced by GM on a dry matter (DM) basis. The GM treatment contained 2–10% higher phytochemical contents than the control. The DMI from the GM treatment was 15% higher than the control (p < 0.001). No difference was found in sheep live weight gain, behaviour, and quality between groups (p > 0.05). No difference was found in total faecal production, faecal organic matter, and nitrogen contents (p > 0.05) and parasitic egg count. The GM treatment led to higher nitrogen intake (23.1 vs. 27.2 g/d) and faecal nitrogen excretion (6.3 vs. 8.7 g/d) compared to the control. Urinary creatinine, allantoin, and purine derivatives were lower in the GM treatment than control (p < 0.05). However, both groups had similar purine derivatives/DMI (i.e., indicator of rumen microbial protein synthesis efficiency; p > 0.05). Overall, the results showed that GM can replace 20% of the control ration to maintain sheep productivity, health, and environmental sustainability.

Volatile fatty acids concentration and efficiency of microbial protein synthesis of concentrate cassava peel diet with different levels of protein sources

This study was conducted to evaluate the effects of different levels of protein sources in a concentrate cassava peel diet on volatile fatty acid (VFA) concentration and efficiency of microbial protein synthesis (EMPS) by in vitro method. The five diet treatments in this study consisted of cassava meal (CM), cassava peel (CP), cassava leaves (CL) and moringa leaves (ML) with the proportion applied were T1=CM70% + CP20% + CL5% + ML5%; T2=CM60% + CP20% + CL10% + ML10%; T3=CM50% + CP20% + CL15% + ML15%; T4=CM40% + CP20% + CL20% + ML20%; T5=CM30% + CP20% + CL25% + ML25%. The experiment used a randomized block design with five treatments and three replications. The results showed that increasing the level of protein sources in the ration tended to increase the value of total VFA, acetate, propionate, CO2, and CH4 in which T3 had the highest values. However, the effect of treatments were significant in EMPS value (P&lt;0.01). It was concluded that increasing levels of cassava and moringa leaves in the concentrate cassava peel diet tended to increase the value of total VFA, acetate, propionate, butyrate, CO2, and CH4 which led to a significant increase in EMPS values.