Efficiency Of Maize Research Articles

Genetic and molecular mechanisms underlying nitrogen use efficiency in maize

Genetic and molecular mechanisms underlying nitrogen use efficiency in maize

Maize (Zea Mays L.) Response to Deficit Irrigation Levels at Different Growth Stages on its Yield and Water Use Efficiency at Koka, Central Rift Valley of Ethiopia

Water scarcity is among the major limitations for crop production. Improving water use efficiency of irrigated crops through water management options is crucial in water scarce areas. Field experiment was carried out at Wondo Genet Agricultural Research Center Koka Research site, to investigate the effect of water stress at different growth stage on yield and water use efficiency of maize. one optimum irrigation and eight growth stage based deficit levels(100% ETC at all growth stages, 75% ETC at all growth stages, 50% ETC at all growth stages, 75% ETC at development growth stage, 50% ETC at development growth stage, 75% ETC at mid growth stage, 50% ETC at mid growth stage, 75% ETC at late growth stage and 50% ETC at late growth stage) were imposed on maize (Zea mays L.) variety Melkassa II as a treatment and laid out in randomized complete block design (RCBD) with three replications. Results indicated that the different levels of growth stage based deficit levels had significant (p<0.01) effect on crop yield, harvest index and water use efficiency. Grain yield reduced with increased stress, whereas water use efficiency was increased with stress level increased. The highest grain yield of 6.4 t/ha and WUE of 1.01 kg/m3 were obtained at 100% ETC and 50% ETC at all growth stages, respectively. Also, 75% ETC at development stage and late stage treatments showed no significant variation with 100% ETC in grain yield. Water use efficiency observed at 75% ETC all growth stages treatment was statistically similar with that of 50% ETC at all growth stages treatment. Grain yield obtained from 50% ETC at mid growth stage was similar with 50% ETC at all growth stages, and water use efficiency was the least and this shows that maize was sensitive to moisture stress at mid growth stage than development and late growth stages. Therefore, maize could be irrigated at 75% ETC at all growth stages and by stressing development or late growth stages up to 50% ETC to increase water use efficiency with a small grain yield reduction for stressed scenario and for non stressed scenario with 100% ETC at all growth stages.

Substituting partial chemical nitrogen fertilizers with organic fertilizers maintains grain yield and increases nitrogen use efficiency in maize

IntroductionLong-term application of excessive nitrogen (N) not only leads to low N use efficiency (NUE) but also exacerbates the risk of environmental pollution due to N losses. Substituting partial chemical N with organic fertilizer (SP) is an environmentally friendly and sustainable fertilization practice. However, the appropriate rate of SP in rainfed maize cropping systems in semi-arid regions of China is unknown.MethodsTherefore, we conducted a field experiment between 2021 and 2022 in a semi-arid region of Northern China to investigate the effects of SP on maize growth, carbon and N metabolism (C/NM), and NUE. The following treatments were used in the experiment: no N application (CK), 100% chemical N (SP0, 210 kg N ha–1), and SP substituting 15% (SP1), 30% (SP2), 45% (SP3), and 60% (SP4) of the chemical N. The relationship between these indicators and grain yield (GY) was explored using the Mantel test and structural equation modeling (SEM).Results and discussionThe results found that the SP1 and SP2 treatments improved the assimilates production capacity of the canopy by increasing the leaf area index, total chlorophyll content, and net photosynthetic rate, improving dry matter accumulation (DMA) by 6.2%–10.6%, compared to the SP0 treatment. SP1 and SP2 treatments increased total soluble sugars, starch, free amino acids, and soluble protein contents in ear leaves via increasing the enzymatic reactions related to C/NM in ear leaves during the reproductive growth stage compared with SP0 treatment. The highest plant nitrogen uptake (PNU) and nitrogen recovery efficiency were obtained under the SP2 treatment, and the GY and nitrogen agronomic efficiency were higher than the SP0 treatment by 9.2% and 27.8%. However, SP3 and SP4 treatments reduced DMA and GY by inhibiting C/NM in ear leaves compared to SP0 treatment. Mantel test and SEM results revealed that SP treatments indirectly increased GY and PNU by directly positively regulating C/NM in maize ear leaves. Therefore, in the semi-arid regions, substituting 30% of the chemical N with SP could be considered. This fertilizer regime may avoid GY reduction and improve NUE. This study provides new insights into sustainable cultivation pathways for maize in semi-arid regions.

Developmental regulators in promoting genetic transformation efficiency in maize and other plants

Given global agricultural challenges such as population growth, climate change, and limitations on resources and the environment, as well as increasing diversity in breeding goals, relying on traditional breeding methods is inadequate to provide food security requirements and promote sustainable development. Genetic transformation technology has become an effective tool for performing functional genomics research and molecular breeding. In this study, we conducted an in-depth analysis of 1669 literary works to investigate the potential of developmental regulators (DRs) in enhancing the efficiency of plant genetic transformation, with a concentration on their use in maize. Through multi-omics data analysis, we identified 12 homologous DRs from various species that are potentially applicable to maize. We identified a total of 41 possible disease resistances (DRs) for maize genetic transformation. Further experimental verification of ZmWIND1, a novel regulator belonging to the ERF/AP2 transcription factor (TF) family, showed that it significantly improved the efficiency of plant regeneration and transformation efficiency in maize. Specifically, compared to the control group, the callus induction rates for the pG3GB411-ZmWIND1 vector increased to 60.22 % and 47.85 % in Xiang249 and Zheng58, respectively. Transformation efficiency increased to 37.5 % in Xiang249 and 16.56 % in Zheng58, both significantly surpassing the control group. These findings have the potential to broaden the range of transformable maize varieties and lines, as well as introduce new genetic transformation methods in agricultural biotechnology, underscoring the immense potential to enhance genetic transformation efficiency through systematic exploration and application of DRs in maize.

The effect of irrigation with magnetized wastewater on soil heavy metals, water productivity and heavy metals in aerial parts and grains of maize

Rising population strains food resources; reusing wastewater increases but brings microbial and heavy metal pollution, impacting nature and human health. Among environmental pollutants, heavy metals in wastewater are a major concern. Using magnetized water is a method to improve water and soil quality. The aim of this research is to investigate the effect of using treated magnetized wastewater on the chemical properties and tracking of heavy metals in the soil, performance and yield components, water efficiency, and absorption of heavy metals by maize plant. Irrigation treatments consisted of various water and wastewater blending ratios under both magnetic and non-magnetic field application conditions. The results showed that the effect of irrigation water and mixing of water and wastewater on electrical conductivity, soil salts and heavy metals in different depths were significant at 1% probability level. On average, irrigation with magnetized wastewater caused a significant increase in grain yield (9.8%) and biological yield of maize (10.63%) compared to non-magnetized wastewater treatment. Irrigation with magnetized wastewater caused a significant increase in biological (10.92%) and physical (10.13%) productivities compared to non-magnetized wastewater treatment. With applying a magnetic field resulted in a reduction of 17.99%, 23.25%, 17.86%, and 17.12% in the concentration of lead, cadmium, zinc, and nickel in the aerial parts of the plant, respectively, compared to the non-magnetized water treatment. Magnetized water increases the water use efficiency of maize and irrigation management with this technology can be useful in more effective and economical use of limited water resources.

Exogenous application of plant bio-regulators improve yield and water use efficiency of maize under drought stress

Moisture stress in rainfed areas has significant adverse impact on plant growth and yield. Exogenous application of plant bio-regulators (PBRs) plays an important role to mitigate drought stress in plants. A field experiment was conducted during rainy (kharif) seasons of 2018 and 2019 to explore the positive role of PBRs on the yield, economic feasibility and water use efficiency of maize grown under rainfed conditions. Ten treatments including PBRs, mulching and control were evaluated in a randomized block design. The result showed that foliar spray of salicylic acid (0.5 mM) with 1% urea solution improved the grain yield of maize by 24% as compared to control plot. Other PBRs such as glycine betaine, gibberellic acid and thiourea also increased the grain yield in the range of 10–21%. Application of 1% urea solution with PBRs synergise the effect of PBR to alleviate drought stress. The foliar applied PBRs maintained higher membrane stability index (73–78%), significantly increased relative water content (5–11%), chlorophyll content and decreased the proline content (11–21%) as compared to plants grown in control plot. Significant increase were noted in the rain and crop water use efficiency of maize with the foliar application of PBRs. Net returns were improved by 33% with application of salicylic acid (0.5 mM) + 1% urea as compared to control (₹49 × 103 /ha). Thus, foliar spray of salicylic acid (0.5 mM) with 1% urea solution could be an economic feasible strategy to reduce the adverse effects of drought and may increase the maize yield and water use efficiency.

Oil-Coated Ammonium Sulfate Improves Maize Nutrient Uptake and Regulates Nitrogen Leaching Rates in Sandy Soil

Ammonium sulfate (AS) has been utilized in agriculture; however, there is a dearth of research on its application in maize cultivation subsequent to the implementation of nitrification inhibitors or coating treatments. This study aimed to analyze the impacts of various combinations of AS fertilizers on soil nutrients, plant nutrient uptake, yield, and fertilizer utilization efficiency in maize cultivation to establish an optimal and stabilized disposal method for AS. A completely randomized design was employed with five treatments (AU, the control using urea; AS, treatment using ammonium sulfate; ASN, treatment using ammonium sulfate with a nitrification inhibitor; ASG, treatment using oil-coated ammonium sulfate; and ASD, treatment using oil–humic acid-coated ammonium sulfate). The results show the following: (1) Compared with AU and AS, ASN, ASG, and ASD decreased the leaching rates of total nitrogen (TN), ammonium nitrogen (NH4+-N), and nitrate nitrogen (NO3−-N), and more residual N had accumulated in the soil. The first-order kinetic equation Nt = N0(1 − e−kt) could better fit the process of N accumulation and release, and the N-release rate constant was in the order of AU > CK > AS > ASG > ASN > ASD. (2) Compared with the AU and AS treatments, the plant dry weight, grain dry weight, spike width, spike length, and yields of maize increased by 8.85–11.08%, 12.98–14.15%, 2.95–3.52%, 5.50–5.65%, and 43.21–51.10%, respectively, under the ASG treatment. A path analysis revealed the main decision coefficient of the effective spike number on the maize yield. Furthermore, the accumulation levels of N, P, and K within above-ground plants significantly increased under the ASG treatment compared with those under the AU and AS treatments. N, P, and K partial factor productivity under the ASG treatment increased by 47.12%, 47.15%, and 73.40% on average, while grain N, P, and K balance increased by 50.45%, 47.10%, 55.61% on average, compared with the AU and AS treatments. Therefore, the ASG treatment exhibited the optimal slow-release effect on nutrients and achieved excellent performance in enhancing the production and efficiency of maize.

Integrated effect of irrigation rate and plant density on yield, yield components and water use efficiency of maize

Integrated effect of irrigation rate and plant density on yield, yield components and water use efficiency of maize

COI1 F-box proteins regulate DELLA protein levels, growth, and photosynthetic efficiency in maize.

The F-box protein Coronatine Insensitive (COI) is a receptor for the jasmonic acid signaling pathway in plants. To investigate the functions of the 6 maize (Zea mays) COI proteins (COI1a, COI1b, COI1c, COI1d, COI2a, and COI2b), we generated single, double, and quadruple loss-of-function mutants. The pollen of the coi2a coi2b double mutant was inviable. The coi1 quadruple mutant (coi1-4x) exhibited shorter internodes, decreased photosynthesis, leaf discoloration, microelement deficiencies, and accumulation of DWARF8 and/or DWARF9, 2 DELLA family proteins that repress the gibberellic acid (GA) signaling pathway. Coexpression of COI and DELLA in Nicotiana benthamiana showed that the COI proteins trigger proteasome-dependent DELLA degradation. Many genes that are downregulated in the coi1-4x mutant are GA-inducible. In addition, most of the proteins encoded by the downregulated genes are predicted to be bundle sheath- or mesophyll-enriched, including those encoding C4-specific photosynthetic enzymes. Heterologous expression of maize Coi genes in N. benthamiana showed that COI2a is nucleus-localized and interacts with maize jasmonate zinc-finger inflorescence meristem domain (JAZ) proteins, the canonical COI repressor partners. However, maize COI1a and COI1c showed only partial nuclear localization and reduced binding efficiency to the tested JAZ proteins. Together, these results show the divergent functions of the 6 COI proteins in regulating maize growth and defense pathways.

Effects of Different Doses of Nitrogen on Growth and Nitrogen Use Efficiency of Maize (Zea mays L.) in Berekum Municipality of Ghana

Background: Adequate and balanced use of plant nutrients could increase crop performance and reduce adverse environmental effects. Farmers in Berekum have little information on the specific rate of nutrient, especially nitrogen application for hybrid and open pollinated maize varieties. Methods: A field experiment was carried out in Berekum Municipality of Ghana to determine the effects of different doses of N fertilizer (0, 90, 120 and 150 kg N ha-1) on growth and nitrogen use efficiency (NUE) of Pannar 12 (hybrid) and Omankwa (open-pollinated) maize varieties. The experiment was conducted in a factorial randomized block design and replicated thrice. Result: Experimental results revealed that incremental doses of nitrogen significantly impacted vegetative growth and NUE of maize. The studies indicated that the growth response in terms of plant height, number of leaves per plant and stem diameter of Omankwa was better than that of Pannar 12. It was found that in the main cropping period of 2019, the maximum NUE of 14.58 kg/kg was gained when Pannar 12 was grown with 150 kg N ha-1, while the highest NUE (15.91 kg/kg) was recorded when Omankwa was grown with 90 kg N ha-1. In the minor season of 2019, NUE was highest (13.54 kg/kg) when Pannar 12 was grown with 150 kg N ha-1, while application of 120 kg N ha-1 to Omankwa resulted in the highest NUE (10.85 kg/kg). The studies indicated that application of nitrogen at 90, 120 and 150 kg N ha-1 improved NUE in maize. However, NUE was optimized in Pannar 12 due to application of 150 kg N ha-1, while application of 90 kg N ha-1 was enough to optimize NUE in Omankwa.

Combined Application of Leguminous Green Manure and Straw Determined Grain Yield and Nutrient Use Efficiency in Wheat-Maize-Sunflower Rotations System in Northwest China.

Leguminous green manure (LGM) has a reputation for improving crop productivity. However, little is known about the beneficial interactions with straw on crop yield and nutrient (N, P, K) use efficiency. Herein, a 9-year field experiment (from 2015 to 2023) containing three treatments-(1) chemical fertilizer as the control (CK), (2) NPK + straw return (Straw) and (3) NPK + straw return with LGM (Straw + LGM)-was conducted to investigate whether the combined application of LGM and straw can increase productivity and nutrient use efficiency in the wheat-maize-sunflower diversified cropping rotation. The results showed that in the third rotation (2021-2023), Straw + LGM significantly increased wheat yield by 10.2% and maize yield by 19.9% compared to CK. The total equivalent yield under Straw + LGM was the highest (26.09 Mg ha-1), exceeding Straw and CK treatments by 2.7% and 12.3%, respectively. For each 2 Mg ha-1 increase in straw returned to the field, sunflower yield increased by 0.2 Mg ha-1, whereas for each 1 Mg ha-1 increase in LGM yield from the previous crop, sunflower yield increased by 0.45 Mg ha-1. Compared to CK, the co-application of LGM and straw increased the N use efficiency of maize in the first and third rotation cycle by 70.6% and 55.8%, respectively, and the P use efficiency by 147.8% in the third rotation cycle. Moreover, Straw treatment led to an increase of net income from wheat and sunflower by 14.5% and 44.6%, while Straw + LGM increased the net income from maize by 15.8% in the third rotation cycle. Combining leguminous green manure with a diversified cropping rotation has greater potential to improve nutrient use efficiency, crop productivity and net income, which can be recommended as a sustainable agronomic practice in the Hetao District, Northwest China.

Methods for Measuring Nutrient Uptake in Maize Using Nitrogen Stable Isotopes.

Nitrogen is a key nutrient for plant growth and development, and understanding nutrient uptake is central to improving nitrogen use efficiency in crops, including maize. Reducing the need for fertilizer without reducing yield is extremely important, as nitrogen fertilizers come with a high environmental cost, in terms of both emissions from manufacturing and losses to waterways or volatilization off fields. Maize develops multiple different root types, including primary, seminal, crown, and brace roots. Part of improving efficiency in maize involves understanding the differences in nutrient uptake via each distinct root type, but these differences have been largely ignored to date. Here, we describe a protocol that uses stable isotopes for determining nitrogen uptake rates by maize root types. We describe the steps both for intact roots, for which we use rhizoboxes with openable front windows that allow access to the roots without disturbing the rest of the plant, and for field-grown plants, for which intact analysis is not feasible and requires excising the roots. The methods described here can also be modified to measure uptake kinetics and for monitoring nutrient translocation between roots and shoots. Advancing our understanding of root physiology and nutrient dynamics will improve breeding opportunities for efficient nutrient uptake varieties, reducing the need for fertilizer additions.

Exploring Nitric Oxide as a Regulator in Salt Tolerance: Insights into Photosynthetic Efficiency in Maize.

The growing issue of salinity is a significant threat to global agriculture, affecting diverse regions worldwide. Nitric oxide (NO) serves as an essential signal molecule in regulating photosynthetic performance under physiological and stress conditions. The present study reveals the protective effects of different concentrations (0-300 µM) of sodium nitroprusside (SNP, a donor of NO) on the functions of the main complexes within the photosynthetic apparatus of maize (Zea mays L. Kerala) under salt stress (150 mM NaCl). The data showed that SNP alleviates salt-induced oxidative stress and prevents changes in the fluidity of thylakoid membranes (Laurdan GP) and energy redistribution between the two photosystems (77K chlorophyll fluorescence ratio F735/F685). Chlorophyll fluorescence measurements demonstrated that the foliar spray with SNP under salt stress prevents the decline of photosystem II (PSII) open reaction centers (qP) and improves their efficiency (Φexc), thereby influencing QA- reoxidation. The data also revealed that SNP protects the rate constants for two pathways of QA- reoxidation (k1 and k2) from the changes caused by NaCl treatment alone. Additionally, there is a predominance of QA- interaction with plastoquinone in comparison to the recombination of electrons in QA QB- with the oxygen-evolving complex (OEC). The analysis of flash oxygen evolution showed that SNP treatment prevents a salt-induced 10% increase in PSII centers in the S0 state, i.e., protects the initial S0-S1 state distribution, and the modification of the Mn cluster in the OEC. Moreover, this study demonstrates that SNP-induced defense occurs on both the donor and acceptor sides of the PSII, leading to the protection of overall photosystems performance (PIABS) and efficient electron transfer from the PSII donor side to the reduction of PSI end electron acceptors (PItotal). This study clearly shows that the optimal protection under salt stress occurs at approximately 50-63 nmoles NO/g FW in leaves, corresponding to foliar spray with 50-150 µM SNP.

Polyaspartic Acid Urea Increased Maize Yield by Enhancing Leaf N Turnover Efficiency and Soil Microbial Diversity

The release rates of different nitrogen (N) fertilizers and their matching with plant demand determine crop yields. A field experiment was conducted to investigate the effect of using no fertilizer (N0), regular urea applied at rates of 180 kg ha−1 (N180) and 240 kg ha−1 (N240), controlled-release urea applied at a rate of 180 kg ha−1 (H180), and polyaspartic acid urea (PASP) on maize seed yield, soil microbial community diversity, and leaf N-converting enzymes. XianYu 688 was selected as the test maize variety. All cobs in the sample plots were collected per unit area to estimate maize yield. The enzyme-linked immunosorbent assay (ELISA) was used to determine leaf N-converting enzyme activities. Soil DNA was extracted using the Power Max Soil DNA Isolation Kit and subsequently sequenced using the Illumina HiSeq platform (PE 2500) to determine the microbial diversity and communities. The results showed that the highest seed yields were obtained under N240 and PASP180 treatments. The N-partial factor productivity of the PASP180 fertilizer was significantly higher than that of the other treatments. PASP treatment significantly increased maize seed yield due to the potential of storing more N in the ear leaves. Additionally, partial N productivity showed a significant positive correlation with the soil microbial Shannon, Chao1, and ACE indices, indicating that increased soil microbial diversity promoted N efficiency in maize. Further analysis revealed that PASP treatment increased seed yield by promoting leaf N-converting enzyme activity and soil microbial diversity. The results revealed that nitrate reductase (NR), glutamate synthase (GOGAT), and glutaminase (GLNS) enzyme activities in maize leaves were higher under the PASP treatment than under other fertilizer treatments. The PASP treatment significantly enhanced soil microbial diversity at different maize stages. Our study revealed the effects of using different N fertilizers on seed yield by examining their impact on soil microbial diversity and leaf N-converting enzyme activity. This study provides essential insights into maize production and soil fertility maintenance in the North China Plain.

Impact of Different Doses of Herbicides on Weed Density and Weed Control Efficiency in Maize

A field experiment was conducted at Chandra Shekhar Azad University of Agriculture and Technology, Kanpur during Kharif season of 2021. To evaluate the effect of different doses of Mesotrione 2.27% w/w + Atrazine 22.7% w/w SC on growth and yield of maize (Zea mays L.). The experiment was laid down in the Randomized block design with ten treatments which was replicated three times. Experimental Field was infested with Echinochloa colona, Digitaria sanguinalis, Dactyloctenium aegyptium, Commelina benghalensis, Trianthema and Cyperus rotundus. Ready-mix herbicide, Mesotrione 2.27% w/w + Atrazine 22.7% w/w SC (Calaris Xtra) manufactured by Syngenta was used in the experiment. Among the herbicidal treatments, the application of Mesotrione 2.27% w/w + Atrazine 22.7% w/w SC @ 1.750 kg a.i. ha-1 recorded the lowest weed density and highest weed control efficiency (approx. 90%) at every growth stage of maize. However, among herbicidal treatments, grain yield of maize was maximum with the application of Mesotrione 2.27% w/w + Atrazine 22.7% w/w SC @ 1.0 kg a.i. ha-1(T4) which might be due do toxicity effect of herbicide.

Modeling N fertilization impact on water cycle and water use efficiency of maize, finger‐millet, and lablab crops in South India

AbstractThe understanding of the impact of nitrogen (N) fertilization on the field water cycle and corresponding water use efficiency (WUE) is very important for optimizing fertilization rates and conserving stressed water resources. We modeled soil moisture dynamics of maize (Zea mays L.), finger millet (Eleusine coracana Gaertn.), and lablab [Lablab purpureus (L..) Sweet] plots using calibrated HYDRUS‐1D model on two experimental sites (rain‐fed and irrigated) for three seasons under different N treatments. The results indicate that the effects of N depended on plant specific properties such as N‐fixation and drought tolerance, and on plant available water content governed by soil structure and rainfall seasonal variability. Maize WUE of plots which received 150 kg/ha of urea (46 N) were 10–30 kg/ha/mm higher than plots which received none; likewise, millet that received 50 kg/ha of urea had a 7–10 kg/ha/mm higher WUE than control plots in both experiments. However, differences in water cycle components were noticeable between N treatments only in the rain‐fed experiment, where higher N levels led to around 60 and 30 mm higher transpiration, 30 and 20 mm lower evaporation, and 30 and 15 mm lower percolation per season for maize and millet, respectively. In 2018, which was the driest year, the difference in maize WUE between the high and low N treatments was only 1 kg/ha/mm, which corresponded with low actual to potential transpiration ratios (). This indicates higher sensitivity of maize to water stress compared to the other crops. The results of lablab indicate a positive impact of N fertilization on WUE only under water‐limited conditions.

Evaluation of urea loaded nanoclay biopolymer composites with Zn and P solubilizing microbes for nitrogen uptake and use efficiency in maize (Zea mays)-wheat (Triticum aestivum) cropping system

A field experiment was conducted during rainy (kharif) 2022 (July 2022–October 2022) and winter (rabi) 2022–23 (November 2022–March 2023) seasons at ICAR-Indian Agricultural Research Institute, New Delhi to evaluate a series of Zn and P solubilizing microbial culture enriched nanoclay biopolymer composite (NCBPC) loaded with nitrogenous fertilizer (urea) and the efficiency of the products for maize (Zea mays L.) and wheat (Triticum aestivum L.). Experiment consisted of 10 treatments, viz. T1, Control; T2, 100% N though urea; T3; T5; T7; and T9, 75% N as urea loaded NCBPC-A (prepared using acrylic acid + acrylamide + mango kernel flour) alone or along with P or Zn or P + Zn solubilizers; T4; T6; T8 and T10, 75% N as urea loaded NCBPC-B (prepared using acrylic acid + acrylamide + maize flour) alone or along with P or Zn or P + Zn solubilizers in a randomized block design (RBD) and replicated thrice. In both maize and wheat crop, highest grain (5.09 and 5.32 t/ha) and straw yield (6.56 and 7.45 t/ha), apparent N recovery (51.26 and 47.26%) and agronomic efficiency (12 and 13.3 kg grain yield obtained/kg N application) were obtained in treatment T10 followed by T9. In addition, total N uptake significantly enhanced by 20.1–28.4% in maize and 22.1–30.8% in wheat (T9 and T10); apparent nitrogen recovery (ANR) improved by 12.9–18.2 and 15.2–21.1% and agronomic efficiency (AE) triggered by 19.5–21.2 and 15.4–20.8% in maize and wheat crops respectively, under T9 and T10 treatments over standard urea fertilization (T2). Thus, the study concludes that, 25% N requirement could be cut down through application of 75% N (urea) loaded NCBPCs in conjunction with Zn or P or Zn + P solubilizing microbial culture as compared to sole urea application under maize-wheat cropping system.

Effect on biomass production and phosphorus use efficiency of maize by using citric acid amended di-ammonium phosphate fertilizer

Phosphorus (P) is a critical factor influencing maize (Zea mays L.) development and yield. To explore the influence of P on maize growth, a pot experiment was conducted during the summer of 2021. There were five treatments T1 = No DAP/CA, T2 = 5 ml (80 mg kg−1 soil) DAP, T3 = 5 ml (120 mg kg−1 soil) DAP, T4 = 5 ml (80 mg kg−1 soil) DAP and 2 mM citric acid, T5 = 5 ml (120 mg kg−1 soil) DAP and 2 mM citric acid. Citric acid-amended P fertilizers (i.e., CA and DAP) exhibited significant effects (p < 0.05) on all the parameters studied in comparison to the sole application of DAP. Among phosphorus fertilized treatments, the DAP 120 + CA produced significantly higher biomass yield (16.37 g pot−1), while sole DAP 80 produced lower biomass (10.61 g pot−1); the DAP 120 + CA produced significantly higher plant length (34.273 cm), while sole DAP 80 produced lower plant length (30.467 cm); DAP 80 + CA produced significantly higher phosphorus recovery (25.583%), while sole DAP 120 produced lower phosphorus recovery (19.863%); DAP 120 + CA showed significantly higher phosphorus use (69.565 mg plant-1), while sole DAP 80 showed lower phosphorus use (39.154 mg plant-1); the highest total PUE (40.503%) was recorded in DAP 120 + CA treatment, while sole DAP 80 treatments showed lower PUE (22.743%); and the highest PCN (4.25 mg g-1) was recorded in DAP 120 + CA treatment while sole DAP 80 treatments showed lower PCN (3.69 mg g-1). In conclusion, the use of citric acid-amended P fertilizers (CA and DAP) significantly improved all parameters studied, compared to the sole application of DAP. The DAP 120 + CA treatment showed the highest values for biomass yield, plant length, phosphorus recovery, phosphorus use, total PUE, and PCN, while the sole DAP 80 treatment showed lower values for these parameters. The findings suggest that growing maize using citric acid-amended DAP could be more beneficial to get a higher crop yield of maize. Thus, fertilizer application in maize agriculture can be a technique for boosting both economic and environmental profitability.

Effect of zeolite and potassium application on nutrient uptake and potassium use efficiency of maize

Effect of zeolite and potassium application on nutrient uptake and potassium use efficiency of maize

CO2 elevation and N fertilizer supply modulate leaf physiology, crop growth and water use efficiency of maize in response to progressive soil drought

AbstractElevated atmospheric CO2 concentration (e[CO2]) and varied nitrogen (N) fertilization levels may mediate the different responses of C4 crops to progressive soil drought. In this study, the effects of reduced N (N1, 0.8 g pot−1) and adequate N (N2, 1.6 g pot−1) supply on leaf physiology, plant growth and water use efficiency (WUE) of maize (C4 crop) exposed to progressive soil drought grown at ambient CO2 (a[CO2], 400 ppm) and elevated CO2 (e[CO2], 800 ppm) concentration were investigated. The results indicated that compared with a[CO2], net photosynthetic rate (An) and leaf water potential (Ψl) at e[CO2] were maintained in maize leaves, while stomatal conductance (gs), transpiration rate and leaf hydraulic conductance were decreased, leading to enhanced WUE from stomatal to leaf scale. Despite An and Ψl of e[CO2] plants were more sensitive to progressive soil drought under both N fertilization levels, e[CO2] would increase leaf ABA concentration ([ABA]leaf) but decline the gs response to [ABA]leaf under N1 supply. e[CO2] coupled with N1 fertilization was conducive to enlarging leaf area, promoting specific leaf area, root and total dry mass, whereas reduced stomatal aperture and plant water use under progressive drought stress, contributing to an improvement in plant WUE, implying a better modulation of maize leaf stomata and water status under reduced N supply combined with e[CO2] responding to progressive soil drought. These findings in the current study would provide valuable advice for N management on maize (C4) crop efficient water use in a drier and CO2‐enriched environment.