Efficiency Of Feed Utilization Research Articles

Effects of dietary multienzymes on the growth performance, digestive enzyme activity, nutrient digestibility, excreta noxious gas emission and nutrient transporter gene expression in white feather broilers.

Adding multienzymes to poultry feed rations is recognized as a nutritional strategy aimed at improving poultry performance and health status. Nonetheless, some literatures present an ongoing debate about the extent of multienzymes beneficial impact on poultry growth performance. This study aimed to explore the impacts of dietary multienzyme supplementation on broilers, focusing specifically on growth performance, carcass characteristics, apparent nutrient digestibility, excreta noxious gas emission, and intestinal nutrient transporter gene expression. A total of 3200 broilers were randomly assigned to five groups (8 replicates per treatment group) and treated with the following: normal control (CON), CON + 100g/t multienzyme (ME100), CON + 150g/t multienzyme (ME150), CON + 200g/t multienzyme (ME200), and CON + 250g/t multienzyme (ME250). Supplementing with multienzymes significantly influenced the feed conversion rate (linear, P = 0.007; quadratic, P = 0.024) and the European broiler index (linear, P = 0.004; quadratic, P = 0.016) in broilers. Dietary multienzymes significantly influenced apparent metabolizable energy (quadratic, P = 0.015) and neutral detergent fiber (quadratic, P < 0.001). Moreover, multienzyme supplementation in the diet also decreased the emission of ammonia (linear, P = 0.001; quadratic, P = 0.006) and hydrogen sulfide (quadratic, P = 0.006) in the excreta. In addition, dietary multi-enzyme notably elevated (P < 0.05) the mRNA expression of nutrient transporter genes, including peptide transporter 1 (PePT1), Na-dependent neutral amino acid transporter (B0AT), glucose transporter 2 (GLUT2), and fatty acid binding protein1 (FABP1). These findings suggest that dietary supplementation with multienzymes can improve the efficiency of feed utilization, and the digestion and absorption of nutrients and reduce excreta gas emission. Furthermore, this study provides a theoretical basis for advancing the use of multienzymes in broiler production.

Pengaruh Penambahan Ampas Tahu Dan Keong Mas (&lt;i&gt;Pomacea canaliculata&lt;/i&gt;) Pada Ratro Pellet Komersil Terhadap Pertumbuhan Ikan Lele (C&lt;i&gt;larias gariepinus&lt;/i&gt;)

The need for fish food is one of the essentials that can determine the level of success of the fish growth and development process. This research aims to analyze the effect of a combination of pelleted feed and animal feed on the growth and survival of catfish (Clarias gariepinus). The research was carried out for 60 days, at the Mataram University fish production and reproduction laboratory using an experimental method with a completely randomized design (CRD) consisting of 5 treatments and 3 replications, namely: A: 100% commercial feed (control), B: Fish Pellets 50% + Golden Snail 25% + Tofu Dregs 25%, C: Fish Pellets 25% + Golden Snails 50% + Tofu Dregs 25, D: Fish Pellets 25% + Golden Snails 25% + Tofu Dregs 50% and E: Fish Pellets 34% + Keong Mas 33% + Tofu dregs 33%. Test parameters include absolute weight, absolute length, specific weight growth rate, specific length growth rate, feed conversion ratio, feed utilization efficiency and survival and water quality. Data were analyzed using Analysis of Variance (ANOVA) and continued with the Duncan test. The results of this study showed that the best growth and survival of catfish was found in the treatment with 34% commercial feed, 33% golden snails and 33% tofu dregs (P4) with an absolute weight of 15.09 g, an absolute length of 7.05 cm , the specific weight growth rate was 3.86%/day, the length growth rate was 1.3%/day, the feed conversion ratio was 1.18, the feed efficiency rate was 85.91% and the survival rate was 73.33%. The proximate test results showed that the highest content was found in the treatment given 25% commercial feed, 50% golden snails and 25% tofu dregs (P2) with a dry content of 36.39%, crude fiber 10.86% and crude protein 31.48%.

Impact of Fish Feed Formulated with Microalgae Biomass in Experimental Tanks with (Oreochromis Niloticus) Tilapia Culture

This project aims to evaluate a fish feed developed in CIATEJ with little modification, which included algal biomass and vegetal protein. This product was tested at the laboratory scale and the pilot-scale upscaling was needed for use with impact assessment studies in real ambient conditions. This food has antioxidant capacity due to the inclusion of microalgae. Additionally, it is nutritionally balanced with plant-based protein sources, partially replacing fishmeal. The feed, compared to commercial feed, has a higher protein content (5%) and lower fat content (3%). Evaluating this feed was of great importance to corroborate its consumption, which can reduce oxidative stress, reduce water contamination, and improve the growth rate and quality of the meat in fish. FCR values of almost 1 were reported here, indicating the more efficient utilization of formulated feed by Oreochromis niloticus. The main component of tilapia meat was moisture 74.31%, followed by protein 14.44%, lipids 6.24%, carbohydrates 0.10%, fibre 1.39%, and ash 3.62%. in experimental feed. In our results in fish filate, we found a 12% increase in protein percentage and a 29% decrease in fat percentage compared to the commercial feed, as expected from the higher protein and lower fat formulated feed. The amount of protein increment in fish filet we achieved in 2 months, the experimental diets significantly influenced the content of polyunsaturated fatty acids at 2.4% while saturated fatty acids were 1.3%. Omega 6 and Omega 9 were 2.33% and 1.73% respectively. The polyunsaturated fatty acid/saturated fatty acid ratio was also higher.

Let’s Shape a Sustainable Future through the Membrane Science and Engineering

Membrane science and engineering are significant in addressing pressing global challenges across diverse sectors. Membrane-based processes enable the efficient utilization of feed, minimize energy consumption, and reduce waste generation, thereby contributing to the development of economically viable and environmentally friendly industrial practices. The universal membrane market 2027 is estimated to achieve 10.1 billion USD with a 9.7% compound annual growth rate (CAGR) (www.marketsandmarkets.com).

Tracking investigation of archaeal composition and methanogenesis function from parental to offspring pigs

Archaea play a crucial role in microbial systems, including driving biochemical reactions and affecting host health by producing methane through hydrogen. The study of swine gut archaea has a positive significance in reducing methane emissions and improving feed utilization efficiency. However, the development and functional changes of archaea in the pig intestines have been overlooked for a long time. In this study, 54 fecal samples were collected from 36 parental pigs (18 boars and 18 pregnant/lactating sows), and 108 fecal samples from 18 offspring pigs during lactation, nursery, growing, and finishing stages were tracked and collected for metagenomic sequencing. We obtained 14 archaeal non-redundant metagenome-assembled genomes (MAGs). These archaea were classified as Methanobacteriota and Thermoplasmatota at the phylum level, and Methanobrevibacter, Methanosphaera, MX-02, and UBA71 at the genus level, involving hydrogenotrophic, methylotrophic, and acetoclastic pathways. The hydrogenotrophic pathway dominated the methanogenesis function, and the vast majority of archaea participated in it. Dietary changes profoundly affected the archaeal composition and methanogenesis function in pigs. The abundance of hydrogen-producing bacteria in parental pigs fed high-fiber diets was higher than that in offspring pigs fed low-fiber diets. The methanogenesis function was positively correlated with fiber decomposition functions and negatively correlated with the starch decomposition function. Increased abundance of sulfate reductase and fumarate reductase, as well as decreased acetate/propionate ratio, indicated that the upregulation of alternative hydrogen uptake pathways competing with methanogens may be the reason for the reduced methanogenesis function. These findings contribute to providing information and direction in the pig industry for the development of strategies to reduce methane emissions, improve feed efficiency, and maintain intestinal health.

Characterization of a novel fast-growing zebrafish: a new approach to growth hormone transgenesis.

The manipulation of the somatotropic axis, governing growth, has been a focus of numerous transgenic approaches aimed at developing fast-growing fish for research, medicine and aquaculture purposes. However, the excessively high growth hormone (GH) levels in these transgenic fish often result in deformities that impact both fish health and consumer acceptance. In an effort to mitigate these issues and synchronize exogenous GH expression with reproductive processes, we employed a novel transgenic construct driven by a tilapia luteinizing hormone (LH) promoter. This approach was anticipated to induce more localized and lower exogenous GH secretion. In this study, we characterized the growth and reproduction of these transgenic LHp-GH zebrafish using hormonal and physiological parameters. Our findings reveal that LHp-GH fish exhibited accelerated growth in both length and weight, along with a lower feed conversion ratio, indicating more efficient feed utilization, all while maintaining unchanged body proportions. These fish demonstrated higher expression levels of LH and GH in the pituitary and elevated IGF-1 levels in the liver compared to wild-type fish. An examination of reproductive function in LHp-GH fish unveiled lower pituitary LH and FSH contents, smaller follicle diameter in female gonads, and reduced relative fecundity. However, in transgenic males, neither the distribution of spermatogenesis stages nor sperm concentrations differed significantly between the fish lines. These results suggest that coupling exogenous GH expression with endogenous LH expression in females directs resource investment toward somatic growth at the expense of reproductive processes. Consequently, we conclude that incorporating GH under the LH promoter represents a suitable construct for the genetic engineering of commercial fish species, providing accelerated growth while preserving body proportions.

Longitudinal modeling of residual carbon dioxide and residual feed intake in the Nordic Red dairy cattle

Feed utilization efficiency is an important trait in dairy production playing a significant role in reducing feed costs and lowering methane emission. One of the metrics used to measure feed efficiency in dairy cows is residual feed intake (RFI). This metric requires routine measurement of feed intake. Since there is a positive high correlation between heat production and carbon dioxide (CO2) production on the one hand and heat production and efficiency on the other hand, residual carbon dioxide (RCO2) might be a useful metric to improve feed efficiency. The objectives of this study were to model the trajectories of RCO2 and RFI as well as to estimate their repeatabilities and correlations at different stages of lactation. Daily CO2 output and feed intake were recorded from 46 primiparous Nordic Red dairy cows using two Greenfeed Emissions Monitoring™ systems from 2 to 305 days in milk (DIM). Edited data comprised 5 995 daily averages. To calculate predicted values of CO2 and DM intake (DMI), prediction models were developed by fitting multiple regression models to observations. Subsequently, RCO2 and RFI were calculated by subtracting predicted values of CO2 and DMI from their corresponding actual observations. A random regression bivariate model was fitted to estimate repeatabilities and animal correlations within lactation at different DIMs between RCO2 and RFI traits. The model fitted included fixed effects of year-month of recording, lactation month, fixed regressions as well as random regressions for the animal effect. The residual variance was considered to be heterogeneous. Repeatabilities and animal correlations of RCO2 and RFI between selected DIM (for every 30 DIM i.e., 6, 36,…, 246 and 276) were calculated. Repeatability of RCO2 was high at the beginning of lactation (0.72 at DIM 6) and decreased around the peak of milk production (0.27 at DIM 96) and again increased gradually toward the end of lactation. Similarly, RFI also had high repeatability at the beginning (0.86 at DIM 6); however, it decreased in mid-lactation (0.37 at DIM 156) and then increased toward the end of lactation. Animal correlations between RCO2 and RFI were moderate to high on the same DIM and ranged from 0.37 to 0.88. Overall, we found that animals with higher CO2 production than expected also consume more DMI than expected, but the moderate correlation between RCO2 and RFI found in this study calls for more research to assess the potential of RCO2 to become a new feed efficiency metric.

The Potential of Fish Protein Hydrolysate Supplementation in Nile Tilapia Diets: Effects on Growth and Health Performance, Disease Resistance, and Farm Economic Analysis.

Fish protein hydrolysate (FPH) has shown immense potential as a dietary protein supplement and immunostimulant in aquaculture, especially in Nile tilapia production. Four isoproteic diets (30% crude protein) were prepared by including FPH at varying percentages (0%, 0.5%, 1%, and 2%). Nile tilapia fed with FPH diets for 90 days, and their growth performance, feed utilization, blood biochemistry, liver and gut morphology, and resistance against Streptococcus iniae were investigated. The findings revealed that diets physical attributes such as pellet durability index and water stability were remarkably (p < 0.05) varied between experimental diet groups. Furthermore, the test diets were more palatable when FPH was included at 1% and 2%. Fish that were fed with a 2% FPH-treated diet had significantly (p < 0.05) greater growth indices than other treatments. Additionally, their feed utilization was significantly (p < 0.05) improved. The experimental diets and intestinal total bacteria count (TBC) exhibited a rising trend with FPH levels, where the 2% FPH-treated diet recorded the highest TBC. Neutrophil (109/L), lymphocyte (109/L), eosinophil (109/L), and red blood cell(1012/L) counts were significantly (p < 0.05) higher in the 2% FPH-treated group, while the white blood cell (109/L), and basophil (109/L) counts were not influenced by the FPH inclusion. Moreover, the FPH-treated groups displayed lower creatinine, bilirubin, and urea levels than the control. The histological examination demonstrated that themid-intestine of 2% FPH-fed Nile tilapia had an unbroken epithelial wall, more villi with frequent distribution of goblet cells, wider tunica muscularis, and stronger stratum compactum bonding than other treatments. Additionally, this group exhibited more nuclei and erythrocytes and less vacuolar cytoplasm in liver than their counterparts. Nile tilapia that were given a diet containing 2% FPH had significantly (p < 0.05) higher resistance (83.33%) to S. iniae during the bacterial challenge test. A significant (p < 0.05) enhancement in farm economic efficiency was observed in the higher inclusion of FPH in diets. In summary, 2% FPH supplementation in Nile tilapia diets improved their growth performance, feed utilization, health status, disease resistance, and farm economic efficiency.

Beneficial effects of American ginseng (Panax quinquefolius L.) extract residue as a feed additive on production, health status, and gastrointestinal bacteria in sika deer (Cervus nippon).

American ginseng residue is an industrial by-product of ginseng saponin extraction, including polysaccharides and amino acids; however, it is often discarded into the natural environment, representing a waste of resources as well as an environmental issue. In this study, we examined the effects of adding American ginseng residue to the basal diet of sika deer. Twelve antler-bearing male sika deer were assigned randomly to groups fed a diet supplemented with 0% (CON), 1% (LGR), and 3% (HGR) American ginseng residue, respectively, (n = 4 per group) for 5 weeks. Supplementation with 3% American ginseng residue significantly increased antler production and feed utilization efficiency in antler-bearing sika deer (p < 0.05). There were no significant differences in serum biochemical indexes among the three groups, but serum immunoglobulin A and glutathione peroxidase levels were significantly increased in the LGR and HGR groups (p < 0.05). Supplementation with American ginseng residue affected rumen fermentation in sika deer, significantly increasing the rumen contents of acetic acid, propionic acid, and total volatile fatty acids, and decreasing rumen fluid pH (p < 0.05), but had no significant effect on microbial protein or ammoniacal nitrogen content. American ginseng residue also affected the rumen bacterial composition, with significant up-regulation of Bacteroidota abundance in the HGR group, significant increases in Fibrobacterota and Fibrobacter abundance in the LGR group, and a significant decrease in Oscillospiraceae_UCG-005. Supplementation with ginseng residue had no significant effect on volatile fatty acids in the feces of sika deer, but did affect the composition of fecal bacteria, with significant decreases in Desulfobacterota and Rikenellaceae_RC9_gut_group in the HGR group, and a significant increase in Ruminococcus in the LGR group (p < 0.05). In addition, the abundance of Paeniclostridium in the feces decreased linearly with increasing concentration of ginseng residue, with a significant difference among the groups (p < 0.05). This study comprehensively evaluated the effects of American ginseng residue as a potential feed additive on the production performance and gastrointestinal bacterial community in antler-bearing sika deer. The results indicated that ginseng residue was a suitable feed additive for improving production performance and health in sika deer.

Effect of Monosodium Glutamate in Artificial Food on Feed Utilization Efficiency, Growth, and Survival of Fingerling Tilapia (Oreochromis niloticus)

Tilapia (Oreochromis niloticus) is one of the leading freshwater fish commodities in Indonesia. The problem often faced by tilapia farmers is the low level of feed utilization, especially protein, which is still low, resulting in suboptimal growth. The glutamate content in monosodium glutamate (MSG) can act as a precursor for the formation of arginine and glutamine, which can increase the production of protease and lipase enzymes that are excreted into the intestines during the feed hydrolysis process, which is useful in increasing the absorption of nutrients in the form of protein and fat. This research was conducted using fingerling tilapia as a test fish with an average individual length of 6.59 ± 0.21 cm/fish and an average individual weight of 5.33 ± 0.27 g/fish. This research was carried out in an aquarium container measuring 40x30x30 cm with a water volume of 27 l and a density of 15 fish/aquarium. The aim of this research was to determine the effect of MSG in fish feed on total feed consumption (TKP), feed utilization efficiency (FUE), protein efficiency ratio (PER), protein retention, relative growth rate (RGR), and survival (SR) in fingerling tilapia. This research used an experimental method with a completely randomized design (CRD) of 4 treatments with 3 replications. Treatments A, B, C, and D are fed with the addition of 0 MSG in commercial feed; 0.75; 1.5; and 2.25 g/kg. Variables observed included TKP, FUE, PER, protein retention, RGR, and SR. The research results showed that MSG in feed had a significant effect (P &lt; 0.05) on the FUE, PER, protein retention, and RGR values but had no effect (P &lt; 0.05) on the TKP and SR values. FUE value; PER, protein retention; The highest RGR was obtained in treatment D (2.25 g/kg); FUE (87.21±2.28); PER(1.83±0.05); protein retention (50.47±0.76); The RGR (3.06 ± 0.03) and survival value for all treatments were found to be 100%. The water quality during the research was in optimal condition.

Effects of Phytase Enzyme Supplementation on Protein Digestibility, Efficiency of Feed Utilization, Growth and Carcass Protein Content of Tambaqui (Colossoma macropomum)

The success of intensive cultivation of tambaqui (Colossoma macropomum) is largely determined by the availability of high-quality feed. High-quality fish diets consist of fish meal produced from animal-based proteins in order to provide fish with the protein requirement. In intensive farming systems, feed costs account for 60–70% of total production costs. Due to fish meal's increasing cost; hence, less expensive ingredients are required to substitute fish meal. Soybean meal can be used as an alternative source of protein to substitute for fish meal due to its lower price and high protein content in fish diet. Phytate acid, one of the antinutritional materials contained within soybean meal, may decrease the nutrient content of feed by affecting with the fish's ability to absorb nutrients. The research aimed to examine the effect of adding the phytase enzyme to diet feed on increasing protein digestibility, efficiency of feed utilization and growth of tambaqui fish. The fingerlings of tambaqui used as test fish had an average weight of 3.85 ± 0.08 g/fish. The diet treatments in this study were phytase enzyme addition in diet with different doses, namely: A (0 FTU/kg feed), B (250 FTU/kg feed), C (500 FTU/kg feed), D (750 FTU/kg feed) and E (1000 FTU/kg feed). The results showed that phytase enzyme addition in diet significantly increased protein digestibility, efficiency of feed utilization, growth, and carcass protein content of tambaqui fish. The most effectiveness phytase enzyme dose for tambaqui fish was 1000 FTU/kg feed, which resulted in highest values of ADCp, PER, FCR, and RGR at 68.46±0.28%, 2.27±0.05, 1.53±0.04, 74.59±0.45%, and 6.01±0.19%/day, respectively.

Influence of nitrogen substitution at an equivalent total nitrogen level on bacterial and fungal communities, as well as enzyme activities of the ditch bottom soil in a rice-fish coculture system.

Rice-fish coculture system (RFS) operates through effectively utilizing water and land resources in a complementary form, but it requires more efficient utilization of fertilizer and feed without compromising rice yield. However, the knowledge of how to regulate the proportion of nitrogen (N) supplied from fertilizer and feed at an equivalent total N levelto improve the benefits of RFS remains limited. Therefore, four treatments (S0: RFS with 0% N fromfertilizer and 100% N from feed; S25: RFS with 25% N from fertilizer and 75% N from feed; S50: RFS with 50% N from fertilizer and 50% N from feed; S75: RFS with 75% N from fertilizer and 25% N from feed) were conducted to assess the variation of ditch bottom soil properties, microbial communities and enzyme activities, as well as to obtain the optimal ratio of N supplied from fish feed and fertilizer. The experiments showed that the contents of soil organic matter, total carbon and total N, and the activities of urease, N-acetyl-β-D-glucosaminidase, protease, β-1,4-glucosidase and catalase in the ditch bottom soil significantly reduced in S25 treatment, compared with the other three treatments. Ammonium N content decreased with increasing percentage of the basal fertilizer, whereas nitrate N content and pH value showed an adverse trend. However, the bacterial and fungal communities were unaffected by the ratio shifts between fertilizer-N and feed-N, but their dominant phyla were influenced by the ditch bottom soil N level. Moreover, the bacterial community composition was positively related to nitrate N, whereas fungal diversity was positively correlated with pH, ammonium N and nitrate N, and urease. We also found that the treatment of N input with 25% N from fertilizer and 75% N from feed can reduce N deposition in the ditch bottom soil in the rice-fish coculture system. Our findings indicate that under the equivalent total N input level, the relative higher ratio of N from fish feed increased (S0 treatment) or reduced (S25 treatment) the deposition of N in the ditch bottom soil, and improved fish production, but decreased rice yield; while the higher ratio of N from basal fertilizer increased the transportation of nutrients into the ditch bottom soil and rice yield, but reduced fish production. So when considering multi-balance and multiple benefits, we recommend that a selective substitution ratio within 50% ~ 75% from fish feed to substitute for the basal fertilizer under the equivalent total N input may achieve a good balance of rice and fish production improvement, and reduce nutrients wastage to the ditch bottom, as well as alleviate the potential of non-point source pollution. This study also provides an evidence for regulating and optimizing the ratio of N supplied from fertilizer and fish feed at an equivalent total N level through monitoring the nutrient accumulation in ditch bottom soil in the rice-fish coculture system. © 2024 Society of Chemical Industry.

Plant-based carotenoid supplementation: Growth, feed utilization efficiency, and coloration in false clownfish (Amphiprion ocellaris)

The false clownfish (Amphiprion ocellaris) is a prominent species in the marine ornamental trade, valued for its vibrant orange-red coloration. However, aquaculture-bred individuals often exhibit less intense coloration than their wild counterparts, presenting a challenge for both breeders and aquarists. This study evaluates the effects of carotenoid-enriched diets, sourced from natural ingredients such as pumpkin, bell pepper, carrot, and gac, as well as a synthetic source like astaxanthin, on the coloration and growth of false clownfish. In a controlled experiment, juvenile fish with an initial average length of 3.21 ± 0.03 cm and weight of 0.61 ± 0.02 g were allocated to 60-liter tanks and fed the experimental diets over a 75-day trial period. Carotenoid supplementation was standardized at 250 mg/kg across diets, with a control group receiving no added carotenoids. The experimental design was completely randomized, involving three replicates per dietary treatment. The results indicated that diets supplemented with gac and bell pepper significantly enhanced growth and feed efficiency (p &lt; 0.05). Notably, the skin redness intensity (a* value) was increased by 75.73% in the gac-supplemented group, 89.20% in the bell pepper group, and 91.99% in the astaxanthin group, relative to the control (p &lt; 0.05). Additionally, carotenoid deposition in the integument was significantly higher in all supplemented groups, with an increase of 83.74% in the astaxanthin group, 89.59% in the gac group, and 98.43% in the bell pepper group, compared to the control (p &lt; 0.05). These findings suggest that natural carotenoids, particularly from gac and bell pepper, can be effective alternatives to synthetic astaxanthin, potentially improving the attractiveness and commercial value of captive-bred false clownfish and alleviating the exploitation of wild populations.

Dietary low‐fat content supplemented with oxytetracycline impairs physiological functions in Nile tilapia (Oreochromis niloticus) fingerlings

AbstractNutrition modulates the vulnerability of animals to xenobiotics insults including antibiotics in cultured fish. However, studies exploring the role of low‐fat diet (LFD) in modulating adverse effects of antibiotics are currently limited. This study explored the physiological effects of feeding LFD supplemented with oxytetracycline (OTC) in Nile tilapia (Oreochromis niloticus) fingerlings. Thirty Nile tilapia (8.64 ± 0.44 g) were tagged and randomly stocked into three tanks and fed on a control diet (CD, 70 g/kg lipid), LFD (20 g/kg lipid) and the LFD supplemented with 2.00 g/kg diet of OTC (80 mg/kg body weight/day), hereafter LFD + OTC for 9 weeks. The results indicated that the Nile tilapia fed on LFD + OTC reduced growth performance and feed utilization efficiency than those fed on CD and LFD. Moreover, the fish fed on LFD + OTC had lower body composition, nutrients digestibility and mesenteric fat index than those fed on CD and LFD. Feeding the fish with LFD + OTC decreased antioxidant capacity in the liver than those fed on CD and LFD. The Nile tilapia fed on LFD + OTC increased hepatotoxicity than those fed on CD and LFD. Feeding the Nile tilapia on LFD + OTC decreased immunity response in the kidney and liver than those fed on CD and LFD. The LFD + OTC affected nutrients metabolism in the liver and serum than other diets. Taken together, feeding LFD with OTC impairs physiological functions of Nile tilapia by inhibiting growth performance, antioxidant capacity, immunity response and nutrient metabolism.

The effect of bromelin in artificial feed on feed utilization efficiency, growth, and survival of gourami seeds (Osphronemus goramy)

The effect of bromelin in artificial feed on feed utilization efficiency, growth, and survival of gourami seeds (Osphronemus goramy)

Modelling the impact of replacing fish oil with plant oils: A meta‐analysis to match the optimal plant oil for major cultured fish

AbstractThe replacement of fish oil with plant oils is a common practice in aquaculture to compensate for the shortage of fish oil supply and has been thoroughly studied. However, because the experimental conditions vary in many aspects, the results are usually inconsistent quantitatively. Therefore, a meta‐analysis was conducted based on a dataset containing 328 articles to systematically model the influence of plant oils on the main aquaculture fish. In addition to demonstrating the species‐specific dose‐dependent impact of plant oils on fish growth, feed utilization efficiency, lipid deposition and health parameters, we have also investigated the role of other factors in this process. We found that the adverse effects caused by plant oils worsen as the experimental duration extends for Atlantic salmon, while Gilthead seabream and European seabass gradually adapt to the change. Further, water temperature could be a decisive factor for Rainbow trout, and early adaptation to plant oils would be helpful for Gilthead seabream. Moreover, sufficient fishmeal supply could alleviate the adverse effects caused by plant oils for most species, but for Gilthead seabream and European seabass, the growth suppression effect of plant oil was larger when fed high‐fishmeal diets. Besides, by comprehensively considering the effects of plant oils, we matched the potential optimal plant oil for each fish species. Our article quantitatively modelled the adverse effects of replacing fish oil with plant oils, investigated the influences of assistant factors and offered a panorama of the research status with emphasis on potential breakthrough orientations.

SESBANIA GRANDIFLORA LEAF EXTRACT ENHANCES GROWTH AND NON-SPECIFIC IMMUNE RESPONSES OF SHRIMP (Penaeus monodon)

This study determined the effects of Sesbania grandiflora (SG) leaf extract on the growth, feed utilization efficiency and non-specific immunological responses in black tiger shrimp (Penaeus monodon). Three diets were prepared by mixing the methanol extract of SG onto a pellet feed (34% protein) at concentrations of 0% (control), 0.05% (T1) and 0.1% (T2), respectively. Ten shrimps (mean weight 2.1g), were reared in each tank of 80 L, and the weight and immunological reaction of shrimp were received after a feeding trail of 28 days. The dietary extract treated shrimp had better growth performance (WG, SGR), and feed utilization efficiency (FCR, PER) compared to the control group. The treated shrimp exhibited enhanced immunological responses; the total haemocyte count, prophenoloxidase activity, superoxide dismutase activity, and shorter haemolymph clotting time compared to the control shrimp. In conclusion, a lower level of SG (0.05%) was found to be most effective in terms of immune response enhancement; hence, this plant could be a good source for feed additives for enhancing sustainable shrimp production.

The Effect of Probiotic-Induced Fermentation of Restaurant Waste on the Growth of Common Carp (Cyprinus carpio) Fry

The study explores using the fermentation process to convert organic restaurant waste into fish feed, a promising solution for aquaculture. The aim is to determine the best type and dosage of probiotics for fermenting restaurant waste (FRWM) and evaluate their impact on common carp (Cyprinus carpio) fry growth. Three commercial probiotics (P1, P2, and P3) were used at an 8% concentration for fermenting the waste. The best result from the fermentation trial was tested as feed containing different FRWM proportions: 0% (TA), 10% (TB), 20% (TC), 30% (TD), and 40% (TE) with 30% protein content. The trial lasted 42 days, assessing waste chemical composition before and after fermentation, growth rate, amylase and protease activity, feed conversion, survival rate, and water quality. Results showed P1 was most effective, increasing FRWM protein content by 84.25% and reducing crude fiber and fat by 15.17% and 3.94%, respectively. TE (40% FRWM) yielded the best outcomes, with fry common carp achieving a 1.05% daily growth rate and a feed conversion ratio of 0.80, indicating efficient feed utilization. TC (20% FRWM) exhibited the highest survival rate at 88.33%. The inclusion of FRWM influenced amylase activity, increasing enzyme levels and improving feed efficiency and growth rate. In conclusion, using FRWM, fermented with P1 probiotics, showed significant improvements in its composition, leading to enhanced fish growth and feed utilization. The 40% FRWM diet demonstrated the best fish growth rate and feed conversion performance. These findings support using fermented restaurant waste as sustainable and nutritious fish feed in aquaculture.

Integrated alternative approaches to select feed-efficient rainbow trout families to enhance the plant protein utilization

Improving feed utilization efficiency is a challenge in aquaculture. Therefore, we developed an indirect benchmark to use in selecting trout for improved efficiency of feed utilization on plant protein (soy)-based diets, with the long-term goal of reducing the cost of commercial trout production. We used a four-part integrative approach to identify feed efficient individuals among 1595 fish coming from 12 genetically selected families by establishing the phenotypic relationship between feed conversion ratio (FCR) and body weight variations using compensatory feeding regimes. Additionally, we examined the nutritional composition of fish filet for each efficiency phenotype during the compensatory regimen. Our findings showed that the fish with the lowest weight loss during a feed deprivation period and the highest weight gain during the refeeding period (FD−/RF +) demonstrated the lowest FCR (FCR = 0.99) and consisted of individuals from several lines. This finding confirms the possibility of improving feed efficiency in mixed lines. Although feeding period has an effect on nutritional composition of fillet, such selection criteria did not show an effect on groups. Overall, successful selection for the improvement of feed efficiency will have a broad application to commercial fish selective breeding programs, leading to increased aquaculture sustainability in the long run.

Layer chicken microbiota: a comprehensive analysis of spatial and temporal dynamics across all major gut sections

BackgroundThe gut microbiota influences chicken health, welfare, and productivity. A diverse and balanced microbiota has been associated with improved growth, efficient feed utilisation, a well-developed immune system, disease resistance, and stress tolerance in chickens. Previous studies on chicken gut microbiota have predominantly focused on broiler chickens and have usually been limited to one or two sections of the digestive system, under controlled research environments, and often sampled at a single time point. To extend these studies, this investigation examined the microbiota of commercially raised layer chickens across all major gut sections of the digestive system and with regular sampling from rearing to the end of production at 80 weeks. The aim was to build a detailed picture of microbiota development across the entire digestive system of layer chickens and study spatial and temporal dynamics.ResultsThe taxonomic composition of gut microbiota differed significantly between birds in the rearing and production stages, indicating a shift after laying onset. Similar microbiota compositions were observed between proventriculus and gizzard, as well as between jejunum and ileum, likely due to their anatomical proximity. Lactobacillus dominated the upper gut in pullets and the lower gut in older birds. The oesophagus had a high proportion of Proteobacteria, including opportunistic pathogens such as Gallibacterium. Relative abundance of Gallibacterium increased after peak production in multiple gut sections. Aeriscardovia was enriched in the late-lay phase compared to younger birds in multiple gut sections. Age influenced microbial richness and diversity in different organs. The upper gut showed decreased diversity over time, possibly influenced by dietary changes, while the lower gut, specifically cecum and colon, displayed increased richness as birds matured. However, age-related changes were inconsistent across all organs, suggesting the influence of organ-specific factors in microbiota maturation.ConclusionAddressing a gap in previous research, this study explored the microbiota across all major gut sections and tracked their dynamics from rearing to the end of the production cycle in commercially raised layer chickens. This study provides a comprehensive understanding of microbiota structure and development which help to develop targeted strategies to optimise gut health and overall productivity in poultry production.