Efficacy Of Liquid-based Cytology Research Articles

Efficacy of Liquid-based Cytology vs Conventional PAP Smear in a Tertiary Care Hospital in Northeast Bihar: A Retrospective Study

Efficacy of Liquid-based Cytology vs Conventional PAP Smear in a Tertiary Care Hospital in Northeast Bihar: A Retrospective Study

Evaluation of Liquid Based Cytology (LBC) and Conventional Pap’s Smear Test as Methods of Cervical Cancer Screening

Background: Cervical cancer screening can actually prevent most cervical cancers and also facilitates early detection which allows fruitful treatment.Objective: To evaluate and compare the efficacy of Liquid-based cytology (LBC) with conventional Pap smear test.Methods: This crosssectional study was conducted between March 2015 and June 2017. A total of 72 women participated in this study attending the Out-Patient Department (OPD) of Gynaecology (Colposcopy Clinic) of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh, for VIA test. The smears prepared by the conventional Pap smear method and Liquid-based cytology method were observed by two independent observers in Department of Pathology of the same institution.Results: The mean age of the patients was 40.22±12.29 years. Considering histopathology as gold standard, in conventional Pap smear preparation our data suggested its sensitivity 87.5%, specificity 92.2%, accuracy 91.7%, positive predictive value 58.3% and negative predictive value 98.3%. In contrast, in Liquid-based cytology, we found its sensitivity 75.0%, specificity 90.6%, accuracy 88.9%, positive predictive value 50.0% and negative predictive value 96.7%.Conclusion: LBC has practical advantages over conventional preparation by producing monolayer of cells, smaller area to screen, reproducibility and availability of doing adjunct molecular techniques from residual sample. However, conventional preparation shows better sensitivity and positive predictive value and almost similar specificity, negative predictive value and accuracy with LBC.International Journal of Human and Health Sciences Vol. 06 No. 01 January’22 Page: 36-40

Liquid-Based Cytology in the Detection of Premalignant Lesions in Patients with "Atypia in Squamous Cells" in Conventional Cytology.

The management of patients with "Atypical Squamous Cells" (ASC) in conventional papanicolaou smears (CPS) is based on the risk of high-grade squamous intraepithelial lesion (HSIL). The efficacy of liquid-based cytology (LBC) to detect this premalignant lesion is variable, with little evidence of its performance in Colombian patients. The aim of this study is to determine the performance of LBC in the detection of premalignant lesions, in patients with ASC in CPS. Were obtained patients who attended colposcopy clinic due the result of ASC in CPS. An LBC was taken, which was interpreted by two pathologists without access to other results. The performance of LBC to detect HSIL, was determined, considering as a gold standard: histopathological study/negative-satisfactory colposcopy. Were included 114 patients, with a mean age of 38.4 years (SD ± 13.3). LBC had abnormal results in 40.36% (n = 46), with a slightly higher proportion of low-grade squamous intraepithelial lesion (LSIL) than HSIL. The total of abnormal diagnoses by colposcopy and/or biopsy was 51.75% (n = 59), with a predominance of LSIL (36.84%). The sensitivity of the liquid-based cytology to detect premalignant lesions was 76.5%, specificity: 66.0%, positive predictive value: 28.3% and negative predictive value: 94.1%; The Cohen's kappa index of LBC for detecting HSIL was 0.2492 for the total population and 0.2907 for ≥30 years. Although LBC decreases abnormal cytology and increases the detection of HSIL, which improves diagnostic accuracy; sensitivity and predictive values for detecting HSIL are not significantly different between CPS and LBC.

Цервикальный скрининг: прошлое, настоящее, будущее

This review aims to summarize currently available information on cervical screening, including screening programs and efficacy of liquid-based cytology and co-testing. We analyzed relevant foreign and Russian publications available in the Pubmed and eLIBRARY electronic databases. Current screening strategies include mandatory HPV testing and cytological examination. HPV self-sampling tests and immunocytochemical examination of cervical smears for P16/Ki67 are also relevant. Screening programs should cover > 70% of the female population. Key words: CIN, cervical cancer, human papillomavirus, cytological method, co-testing, Pap test, self-sampling test

Comparison of liquid-based cytology with conventional smear cytology for EUS-guided FNA of solid pancreatic masses: a prospective randomized noninferiority study

There are limited data on the efficacy of liquid-based cytology (LBC) for EUS-guided FNA specimens. We aimed to evaluate the diagnostic efficacy of LBC for solid pancreatic neoplasms compared with conventional smears (CSs). In this randomized, crossover, noninferiority trial, we randomly assigned (1:1) patients with suspected pancreatic cancer to the LBC group or the CS group. Aspirates from the first needle pass were processed by one method, aspirates from the second pass by the other method, and specimens from the last pass were processed as core biopsy samples. The primary endpoint was the diagnostic efficacy of each method, with the final diagnosis as the gold standard. A noninferiority margin of-10% was assumed. Of 170 randomized patients, 165 were classified as malignant and 5 as benign. Unsatisfactory samples were less frequent in the LBC group (1.78%) compared with the CS group (5.33%). The diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of LBC versus CS were 88.0% versus83.8% (P= .276), 87.7% versus 83.2% (P= .256), 100% versus 100% (P= .999), 100% versus 100% (P= .999), and 16.7% versus 16.1% (P= .953), respectively. A bloody background was significantly more frequent in the CS group (CS, 85.2%; LBC, 1.8%; P< .001), whereas the nuclear features were similar for both groups. The diagnostic usefulness of LBC was comparable with that of CS. The cytomorphologic featuresdid not differ significantly between the 2 methods, and the reduced bloody backgrounds allowed bettervisibility in the LBC method. (Clinical trial registration number: NCT03606148.).

Diagnostic efficacy of liquid-based cytology in endoscopic ultrasound-guided fine-needle aspiration for solid pancreas lesion: Single academic institution experience

Diagnostic efficacy of liquid-based cytology in endoscopic ultrasound-guided fine-needle aspiration for solid pancreas lesion: Single academic institution experience