Chronic low back pain (CLBP) is prevalent among IT professionals and often accompanied by psychological symptoms such as depression, stress, and sleep disturbances. While conventional treatments primarily target pain management, holistic approaches like yoga show promising results in addressing both physical and psychosomatic factors. This randomized controlled trial (RCT) aims to assess the impact of a yoga intervention on depression, stress, and sleep quality in IT professionals with CLBP. A total of 83 IT professionals with CLBP were randomly allocated to either a yoga (n = 40) intervention group or a control group (n = 40) receiving standard care. The intervention group underwent a structured yoga program tailored for CLBP, while the control group followed a standard care protocol. Outcome measures, including pain intensity, depression levels, perceived stress, and sleep quality, were assessed using validated standard questionnaires. Preliminary analysis reveals significant enhancements in the yoga intervention group compared to the control group. Specifically, participants in the yoga group reported a statistically significant reduction in pain intensity (p < 0.05), along with notable decreases in depression scores (p < 0.05), anxiety score (p < 0.05), and stress levels (p < 0.05). Additionally, the yoga intervention group demonstrated enhanced sleep quality (p < 0.05) compared to the control group. This RCT provides compelling evidence for the efficacy of yoga as an adjunctive therapy for CLBP management in IT professionals. The findings emphasize the potential of yoga in alleviating both physical and psychological symptoms associated with CLBP. Healthcare providers may consider incorporating yoga into treatment programs for CLBP in IT professionals to optimize outcomes and enhance overall well-being. Moreover, these results emphasize the importance of holistic interventions in addressing the complex nature of CLBP in the workplace.