Effects Of Various Extracts Research Articles

COMPARATIVE ANTI-TRICHOMONAS VAGINALIS ACTIVITY EVALUATION OF SAMBUCUS NIGRA L. FLOWERS AND FRUITS EXTRACTS

Background: Sambucus nigra or also known as Black elderberry or European elderberry is been used for the teatment and prevention of various diseases. The plant posseses health beneficial effects such as antimicrobial, antioxidant, antiulcerogenic, antidepressant, antidiabetic, antiviral and antiinflammatory. However, there is limited report on the antitrichomoniasis activity of S. nigra. Objectives: The study aimed to comparatively analyze the in vitro trichomonicidal effects of various extracts prepared from both fruits and flowers of S. nigra. Half maximal inhibitory concentration (IC50) and minimum parasiticide (MPC) values of the extracts against metronidazole-resistant Trichomonas vaginalis 50143 strain were determined by the liquid microdilution method using 96-well microplates. Results: After 48 hrs incubation with parasites, the highest activities were observed for 70% hydroethanolic extract, chloroform extract and methanol extract of fruits with IC50 values of 513, 531 and 566 µg/ml, respectively. Fruit extracts exhibited higher activity than flower extracts, and no activity was observed in methanol extract of flowers. Conclusion: This study is the first comparative analysis of the anti-Trichomonas vaginalis activity of elderberry extracts. This study will serve as a reference source in isolating the components of elderberry fruits with antiparasitic activity. Peer Review History: Received 23 March 2024; Revised 6 May 2024; Accepted 27Metronidazole June; Available online 15 July 2024 Academic Editor: Dr. Amany Mohamed Alboghdadly, Ibn Sina National College for Medical Studies in Jeddah, Saudi Arabia, amanyalboghdadly@gmail.com Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my Dr. George Zhu, Tehran University of Medical Sciences, Tehran, Iran, sansan4240732@163.com

Comprehensive Review of Cyclamen: Development, Bioactive Properties, and Therapeutic Applications.

Plants are being researched as potential sources of novel drugs, which has led to a recent acceleration in the discovery of new bioactive compounds. Research on tissue culture technology for the synthesis and processing of plant compounds has skyrocketed, surpassing all expectations. These plants can be bought either raw or as extracts, where some of the chemicals are extracted by mashing the plant in water, alcohol, or another solvent. The use of herbal medicine may open new chances for reducing the onset of infections and treating different diseases including cancer. A perennial plant that blooms in the winter, Cyclamen, is one of the most widely used potted flowers in many nations. Alkaloids, flavonoids, phenols, tannins, saponins, sterols, and glycosides are the main active components of Cyclamen. Analgesic, cytotoxic, antioxidant, antimicrobial, and anti-inflammatory properties have all been demonstrated as potential effects of various extracts of Cyclamen tubers. However, the use of this medicinal plant in official medicine will require further research in the areas of pharmacology. Furthermore, it is necessary to create standard operating procedures for a crude herbal medication. In this regard, this review aims to highlight the key characteristics of the Cyclamen plant, such as its various parts, species, stages of development, and geographic range; pinpoint its intriguing bioactivities, its antioxidant, anti-inflammatory, and its anti-cancerous effects; and ascertain its potential medicinal uses and the main future perspectives.

Investigation of anti-diabetic effects of various extracts of Jatropha tanjorensis in BRIN-BD11 clonal pancreatic beta cells

Investigation of anti-diabetic effects of various extracts of Jatropha tanjorensis in BRIN-BD11 clonal pancreatic beta cells

Evaluation of the anti-obesity effect of Sambucus nigra L. (elderberry) and Vitex agnus-castus L. (chasteberry) extracts in high-fat diet-induced obese rats.

The aim of this study was to investigate the effects of S. nigra L. and V. agnus-castus L. plants on obesity in vivo. Extracts were prepared from S. nigra leaves, flowers, fruits and from V. agnus-castus leaves, flowers, and fruits using 100% water and 70% ethanol. The total phenol and flavonoid contents of the extracts were quantified spectrophotometrically. The findings revealed that the ethanol extracts of V. agnus-castus and S. nigra flowers had the highest phenolic content, while the ethanol extracts of S. nigra flowers and V. agnus-castus leaves had the highest flavonoid content. Qualification and quantification of the phenolic contents of the extracts were carried out using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses. The study investigated the effects of various extracts on plasma levels of leptin, insulin, triiodothyronine (T3), thyroxine (T4), triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and lipase enzyme in obesity-induced rats. The results showed that the ethanol extract of V. agnus-castus flowers, as well as the ethanol and water extracts of V. agnus-castus leaves, resulted in body weight reduction in rats with obesity. Additionally, these extracts were found to decrease serum levels of LDL, triglycerides, leptin, lipase, TNF-α, and IL-1β while increasing levels of HDL and adiponectin. The LC-HRMS results demonstrated that all three extracts exhibited relatively high concentrations of luteolin-7-glycoside and kaempferol, in comparison to the other extracts. The ethanol extract of V. agnus-castus flowers contained 653.04mg/100g of luteolin-7-glycoside and 62.63mg/100g of kaempferol. The ethanol extract of V. agnus-castus leaves contained 1,720.26mg/100g of luteolin-7-glycoside and 95.85mg/100g of kaempferol. The water extract of V. agnus-castus leaves contained 690.49mg/100g of luteolin-7-glycoside and 194.41mg/100g of kaempferol. The study suggests that the ethanol extract of V. agnus-castus flowers and leaves, as well as the water extract of V. agnus-castus leaves, may have potential benefits in treating obesity. However, further controlled clinical studies are necessary to evaluate the clinical efficacy of V. agnus-castus in treating obesity and investigate the in vivo anti-obesogenic effects of luteolin-7-glycoside and kaempferol separately, both in their pure form and in combination.

Comfrey (Symphytum officinale L.) root oleaginous macerate: preparation and antimicrobial action

Medicinal comfrey (Symphytum officinale L.) preparations have anti-inflammatory, antimicrobial and pain-relieving effects, so extracts from the roots of comfrey can be incorporated into various preparations for local effects. Clinical studies were confirmed the antimicrobial activity of the plant. But there is still a need to study the antimicrobial effect of various extracts and preparations from this plant. Research problem - what extraction method will ensure the antimicrobial effect of an oil extract of comfrey? The purpose of the study: to select the method of extracting the raw material of the roots of comfrey with oil, to determine and compare the antimicrobial effect. The maceration methods were applied to the extraction of the comfrey: classic and modified. The results showed that the method of extraction from the plant raw material and the modifications of the method have an effect on the quality and effect of the extracts. Based on the result, it can be stated that maceration at higher than room temperature reduced the antimicrobial activity of the obtained macerate.

Semi Automated Movement Based Screening of Aqueous Extracts of Cinnamon Leaf, Almond, Beetroot Carrot, Ashwagandha and Tulsi on Caenorhabditis Elegans Model System for Health Beneficial Impact

The research was conducted at University of Agricultural Sciences Raichur during 2021-22. In this research Caenorhabditis elegans is utilized by the researchers to know the effects of various extracts, drugs, nano particles, xenobiotics, magnetic effects on behaviour, neuronal effects, feeding pathways, apoptosis, ageing related pathways. Experiments were performed to evaluate the traditional herbs (Cinnamon, Almond, Beetroot Carrot, Ashwagandha and Tulsi) consumption for their health beneficial effects by monitoring the movement as an end point. Movement of an organism is considered as the health and age influencing factor. As the movement is well maintained in organism means health is also well maintained. Hence consistent movement is the factor for the healthy life. In the present study the organism movement has been monitored from L4 onwards every day for 30 minute till 21st day of the survival by automated system. The worm movement during this period has been categorised based on activity in three categories from L4 to 4days the activity ranged from 28.0 units to 10 IR units, 5th to 15th day 27.82 to 14.67 and from 16th to 21st day ranged from 17.87 to 14.44 IR units in non exposed N2 wild type worms. There is a gradual increase in the activity has been recorded on the exposure to health beneficial extracts in a single dose of supplementation. Hence, the study concludes that the health supplements can impact on the move ability of worms and maintains consistent movement till 21 days. Further studies are required to establish comprehensive health beneficial effects of these extracts at organism level.

Application of In Silico Analysis to Determine Morphogenesis in Plant Tissue Culture

The work proposed and tested a new approach to optimizing biotechnological processes, including the process of microclonal propagation. The proposed method is based on constructing a map of the similarity of the structures of molecules of secondary metabolites of plant extracts and molecules that regulate the processes of plant morphogenesis (primarily phytohormones) with subsequent prediction of the action of the extract. Lichen extract of Cetraria islandica (L.) Ach. (Parmeliaceae)was used as an example, for which the range of secondary metabolites contained is well known. The structural similarity of aliphatic secondary compounds of lichen (protolichesteric and lichesteric acids) with strigolactones (to a greater extent), as well as with gibberellins and brassinosteroids, was revealed. Based on the analysis of the results obtained, a prediction was made about the dose-dependent effect of the lichen extract of C. islandica on growth processes and rhizogenesis of microshootsin vitro. This hypothesis was experimentally tested in experiments with microclonal propagation of higher plants Lonicera caerulea L. and Populus tremula L. As a result of the work carried out, it was established that the addition of extract from C. islandicaat a concentration of 10–50 mg/L in the nutrient medium increased the multiplication rate of L. caerulea (by 31%) and P. tremula (by 8%). The rhizogenic activity of the lichen extract at the same concentrations (10–50 mg/L of medium), similar to the activity of strigolactones and gibberellins, has been experimentally proven. The extract has also been shown to have a positive effect of C. islandica (50 mg/L) on elongation of microshoots of both cultures and hemogenesisof P. tremula.The proposed approach allows for optimizing studies aimed at identifying the effect of various extracts on plant morphogenesis in vitroby preliminary constructing a map of the similarity of secondary metabolites contained in extracts (including according to literature data) and known growth regulators (including phytohormones) with subsequent prediction of the effect of the extract.

Clinical and pharmacological aspects of vervain (Verbena officinalis L.) using in the treatment of respiratory system diseases (literature review)

In the pharmacotherapy of the respiratory system diseases, phytotherapy attracts attention due to the high preventive and therapeutic potential of biologically active substances of medicinal plants. At the same time, verification of the effectiveness and safety of herbal drugs is necessary. That is why scientific research of folk medicine plants including common verbena (Verbena officinalis L.) of the Verbenaceae family has been carried out.
 The aim of the work is the analysis of verified data on the chemical composition of the herb verbena, the results of pharmacological and toxicological studies directed at determining the effectiveness and safety in the treatment of infectious and inflammatory diseases of the respiratory tract.
 The search for verified data using Internet resources, comparative analysis, systematization and generalization of data were carried out.
 The main secondary metabolites that determine the profile of verbena herb biological activity are iridoid glycosides (verbenalin, aucubine and gastatoside), as well as phenylpropanoid glycosides (verbascoside, isoverbascoside and eukovoside). Thus, the antibacterial and antifungal activity of certain verbena extracts against clinically significant strains of bacteria and fungi has been proven. The anti-inflammatory effect of various extracts of the Verbena herb was established on carrageenan-induced and formalin-induced edema models. The antioxidant effect of certain verbena herb fractions has been proven by in vitro methods. It is important at presence of diseases for which oxidative stress is an important pathogenetic factor. The results of clinical studies have confirmed the anti-inflammatory and antibacterial activity of the vervaine herb decoction applied topically in the case of chronic gingivitis. The expediency of verbascoside use for increasing the barrier functions of the oral mucosa, promoting epithelization, reducing pain and other signs of inflammation in oral mucositis against the background of tumour chemotherapy has been substantiated. Toxicological studies have shown that different verbena herb extracts belong to the class of low-toxic or practically non-toxic substances when taken orally.
 Thus, the antimicrobial, anti-inflammatory and antioxidant effects of verbena, as well as its high level of safety, substantiate the prospects for the use of this medicinal plant in the treatment of the respiratory system diseases.

LCMS Determination and Cytotoxicity of Abrus precatorius on L6 and SK-N-MC Cell Lines.

The present study aimed to investigate the cytotoxic effect of various extracts derived from Abrus precatorius Linn. leaves on rat L6 and human SK-N-MC neuroblastoma cell lines and determine the secondary metabolites responsible for the cytotoxicity of Abrus precatorius. Successive solvent extraction of A.precatorius leaves was carried out using the Soxhlet apparatus with solvents such as petroleum ether, chloroform, ethyl acetate, and ethanol. HPTLC fingerprinting and LC-MS studies were performed to assess the presence of secondary metabolites, such as flavonoids and phenols, in the ethyl acetate extract. Furthermore, the cytotoxic effect of extracts was tested on rat skeletal muscle cell line L6 and human neuroblastoma cell line SK-N-MC using MTT assay. The total phenolic content of ethyl acetate and ethanol extracts of A.precatorius were 72.67 and 60.73 mg, respectively, of GAE/g dry weight of the extract. The total flavonoid content of ethyl acetate and ethanol extract of A.precatorius were 107.33 and 40.66 mg of Quercetin equivalents/g dry weight of the extract. LCMS analysis demonstrated that the flavonoids in specific Naringenin, Diosmetin, Glycitin, and Genistein might play a prominent role in the cytotoxicity of A.precatorius. The cytotoxicity study revealed that the extracts of A.precatorius were non-toxic to rat L6 myotubes, and the IC50 values of the various extracts, such as APPE, APCH, APEA, and APET, were >100 µg/ml. The extracts exhibited cytotoxic activity against human neuroblastoma SK-N-MC cells, and the IC50 values of APPE, APCH, APEA, APET, and the standard drug "Cisplatin" were >100, >100, 64.88, >100, and 3.72 µg/ml, respectively. It was concluded from the study that the extracts of Abrus precatorius were cytotoxic to neuroblastoma cell lines but non-toxic to normal cell lines. HPTLC and LC-MS studies confirmed that flavonoids in the ethyl acetate extract could be responsible for the biological activity.

Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts.

Surgical site infection remains a devastating and feared complication of surgery caused mainly by Staphylococcus aureus (S. aureus). More specifically, methicillin-resistant S. aureus (MRSA) infection poses a serious threat to global health. Therefore, developing new antibacterial agents to address drug resistance are urgently needed. Compounds derived from natural berries have shown a strong antimicrobial potential. This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry (Rubus chamaemorus) and raspberry (Rubus idaeus), on the development of an MRSA biofilm and as treatment on a mature MRSA biofilm. Furthermore, we evaluated the ability of two cloudberry seed-coat fractions, hydrothermal extract and ethanol extract, and the wet-milled hydrothermal extract of a raspberry press cake to inhibit and treat biofilm development in a wound-like medium. To do so, we used a model strain and two clinical strains isolated from infected patients. All berry extracts prevented biofilm development of the three MRSA strains, except the raspberry press cake hydrothermal extract, which produced a diminished anti-staphylococcal effect. The studied arctic berry extracts can be used as a treatment for a mature MRSA biofilm, however some limitations in their use exist.

Green synthesis and characterization of silver nanoparticles from Nephelium lappaceum fruit peel

In the present work, the qualitative analysis of Nephelium lappaceum fruit peel was performed from aqueous, ethanol and methanol extracts. The phytochemicals present in the Nephelium lappaceum fruit peel were alkaloids, steroids, tannins, soponins, terpenoids, phenols and flavonoids. Silver nanoparticles produced by green synthesis using fruit peel extracts as reducing agents have photocatalytic properties. The nanoparticles synthesized fruit peel was studied and characterized through UV-Visible spectroscopy and FT-IR. Seven pathogens, E. coli, P. aeruginosa, S. typhi, Klebsiella sp, Bacillus sp, S. aureus and Proteus sp and two fungal pathogens, Candida sp and Fusarium sp, were tested for antibacterial activity of Nephelium lappaceum fruit peel aqueous, ethanol and methanol extracts. Total antioxidant capacity, DPPH, reducing power and ABTS were used to test the free radical scavenging activities of Nephelium lappaceum fruit peel extract. Membrane stabilisation and heat induced hemolysis, were used to test the anti-inflammatory effects of various extracts of Nephelium lappaceum fruit peel. In comparison to aqueous and ethanol extracts, methanol extracts had the highest anti-inflammatory efficacy.

Antifungal effects of various extracts from three Artemisia species against dermatophytosis fungal agents

Antifungal effects of various extracts from three Artemisia species against dermatophytosis fungal agents

Contraceptive efficacy and antioxidant potential of Leptadenia reticulata bark extracts in male albino rats

Birth control measures available are primarily for women which are hormonal supplements that are increasing cancer risks and reproductive health issues. Male contraceptive options are effective and available, i.e. barrier methods and vasectomy. Condoms are failure-prone and single-use, while a vasectomy is a permanent sterilization method done surgically, and reversion is not always successful and expensive. A promising oral male contraceptive drug candidate is yet to be discovered. This study investigated the contraceptive efficacy and antioxidant potential of various extracts of Leptadenia reticulata bark in male rats. To study the effects of various extracts (ethanolic and petroleum ether) of L. reticulata bark in male rats, oral administration at the dose level of 250 mg/kg body weight/ day was done for 60 days. Observations were made for body and organ weight, hematology, serum biochemical chemistry, testosterone and antioxidants, lipid profile, sperm parameters (density and motility) and histological changes (reproductive organs). As compared to control in treated groups (TP and bark petroleum ether extract), a significant reduction (P ≤ 0.001) was perceived in sperm motility and density, as well as reproductive organ weight, serum testosterone, and serum antioxidant parameters like SOD. Histological observations revealed arrest in spermatogenesis and reduced seminiferous tubule diameter, mature Leydig cells, secondary spermatogonia, and spermatids which caused a substantial increase in LPO and GSH. From the research findings, it can be concluded that bark petroleum ether extract of L. reticulata possesses contraceptive potential in male albino rats and can serve as a safe and reversible oral contraceptive for males.

Allelopathic effect of phenolic acids in various extracts of wheat against Fusarium wilt in faba bean.

Allelopathy is the main reason for disease control in intercropping systems. The effects of different extracts, root secretions and phenolic acids of wheat and faba bean on Fusarium oxysporum f. fabae (FOF) growth were studied to explore the allelopathy mechanism of wheat in disease control of faba bean. Various extracts and root exudate of faba bean were promoted but those of wheat inhibited the growth and reproduction of FOF. High-performance liquid chromatography revealed significant differences in the contents of phenolic acids in the various extracts and root exudate of wheat and faba bean. The total content of syringic acid (SA) was much higher, but that of other five phenolic acids were lower in wheat than in faba bean. The in vitro addition of these phenolic acids revealed that cinnamic acid (CA), p-hydroxybenzoic acid (PHBA), benzoic acid (BA), vanillic acid (VA) and ferulic acid (FA) exhibited significant promoting effects and SA exhibited strong inhibitory effects on the growth of FOF. These results suggest that the inhibitory effect of various extracts and root exudates from wheat on FOF growth may be due to differences in phenolic acid content and high levels of SA.

A Review on the Cytotoxicity and Antibacterial Effect of Marine Organisms of Persian Gulf.

Marine organisms contain several natural products and bioactive compounds, including hydrolyzed proteins, antioxidant peptides, gelatin, collagen, ω-3 unsaturated fatty acids, vitamin A, vitamin D, calcium phosphate, hydroxyapatite, chitosan, lectin, and various toxins. They can inhibit diverse diseases, be used in pharmaceutical compounds, or as antibiotics and pigments. In this regard, these microorganisms are of crucial medicinal and economical importance. Thanks to new technologies and advanced laboratory methods, bioactive compounds can be extracted from aquatic organisms. In this review study, the cytotoxicity (IC50) and antibacterial effect of various extracts from marine organisms of the Persian Gulf are explored, compiled, and compared. Due to their easy accessibility, most of the studies are green, red, and brown algae.

Phytochemical Profiling, Antioxidant and Anti-Inflammatory Activity of Plants Belonging to the Lavandula Genus.

Lavender is a valuable medicinal plant belonging to the Lamiaceae family. Currently 39 species are known, but only Lavandula angustifolia is a pharmacopoeial raw material. Lavender has a long history of medicinal use and mainly exhibits antioxidant, anti-inflammatory, sedative, antidepressant, spasmolytic, anticholinesterases, antifungal and antibacterial properties. Used internally, it relieves symptoms of mental stress and insomnia and supports digestion. Topical use of lavender in aromatherapy, neuralgia and antiseptics is also known. The constant interest in lavender, and in particular in Lavandula angustifolia, in the field of medicine and pharmacy is evidenced by the growing number of publications. In view of so many studies, it seems important to review traditional and modern extraction techniques that determine the chemical composition responsible for the antioxidant and anti-inflammatory effects of various extracts from the species of the Lavandula genus.

In Vitro Anti-microbial effect of various extracts of Ġokṣura (Tribulus terrestris) fruits on common pathogens causing Urinary Tract Infection

Introduction: The present study was carried out with an objective to investigate the antimicrobial potentials of various extracts of Gokṣura (Tribulus terrestris Linn.) fruits on common uropathogen strains. Material and Methods: Aqueous, ethanol, chloroform, petroleum ether extracts of fruits of Tribulus terrestris were evaluated for potential antimicrobial activity against certain uropathogen strains. The antimicrobial activity was determined in the extracts using agar well diffusion method. The antibacterial activities of extracts (5%, 10% and 15% w/v) of Tribulus terrestris were tested against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumonia and Enterococcus faecalis. Zone of inhibition of extracts were compared with that of standard drug Azithromycin 1 % w/v (Positive control) and DMSO(Negative control) for antibacterial activity. Observations and Results: Inhibition of the bacterial growth was shown against the tested organisms in all extracts but ethanol extract at 15% concentration showed highest activity against all pathogens. The phytochemical analyses of the plants were also carried out which was found to be similar to standard values of API. Conclusion: The results of this study showed that Tribulus terrestris possesses significant antibacterial activity against common uropathogens.

Comparative Investigation of Hair Shampoos Formulated Using Different Herbal Extracts

Cosmetics have fascinated the human race for ages. Cosmetics have been found to benefit in several ways such as enhancing appearance, protection, cleansing etc. If fortified with the benefit of herbal ingredients, these personal care products have proven to be a boon for many. The current study involves the formulation of seven shampoos using different herbal extracts and their comparative investigation for physicochemical and performance characteristics. Various herbal extracts using components such as coffee, fenugreek, green tea, bringaraj, onion seeds, orange oil, and lemon oil were developed and compared against the marketed standard. The effect of various extracts on the parameters such as surface tension, foam test and foam stability test, viscosity, cleansing test, sag test, dirt dispersion, wetting time and pH was successfully tested. All the formulations were found to be beneficial in eliminating oil, dirt, and dandruff, encouraging smooth, silky hair appearance, fortifying and darkening hair without altering or harming hair tresses. Our findings suggest the incorporation of fenugreek, lemon and orange peels to increase the cleansing ability. However, the inclusion of onion seeds and green tea has been found to be beneficial in the generation and stability of the foam.

Anti-Inflammatory and Antioxidant Effect of Various Extracts of Coleus forskohlii Root

Currently, medicinal plants are attracting a great deal of interest as sources of chemicals with antioxidant and anti-inflammatory characteristics. Concerning inflammatory processes and oxidative stress, we evaluated the possible therapeutic benefit of several extracts (methanolic, ethanolic, aqueous, and chloroform) of Coleus forskohlii root. This research used the in vitro antioxidant model as DPPH radical scavenging and anti inflammatory action as protein denaturation and HRBC membrane lysis. For this objective, a rather novel strategy based on the measurement of anti-inflammatory and antioxidant characteristics in combination was used. Using in vitro models, the methanolic extract of Coleus forskohlii root has the highest antioxidant and anti-inflammatory activity, followed by ethanol, aqueous, and chloroform extracts. The root of Coleus forskohlii might serve as a promising material for the creation of innovative and safer plant-based anti-inflammatory and antioxidant medicines.

ANTICANCER ACTIVITY OF NATURAL COMPOUND FROM LEMON GRASS LEAVES EXTRACT ON LUNG CARCINOMA CELL LINE A549: AN IN VITRO STUDY

Lemon grass is a widely cultivated plant, whose extracts are known to possess anti-cancer properties. However, studies related to the effect of different solvent extracts of lemon grass on lung cancer cell lines are scarce. This study was conducted to study the effect of various extracts on the viability of the A549 lung carcinoma cell line. Four solvents (hexane, chloroform, ethyl acetate and ethanol) were used for extraction of lemon grass. The effect of these solvent extracts on A549 cell line was studied. Cell viability was studied by 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) method. The effect of apoptosis on exposure to aforementioned extracts was observed with help of nuclear staining assays and western blotting techniques. Apoptosis was confirmed by nuclear staining using acridine orange ethidium bromide (Ao/EtBr), while pro- and anti-apoptotic cells expressions analysis was conducted using western blotting. All extracts showed cytotoxicity on lung carcinoma cell line, wherein, ethyl acetate and hexane extracts showed up to 48.6±3.8% and 51.7±1.7% viability at 250µg/mL concentration. Up regulation of pro-apoptotic gene expressions like Caspase-3 and inhibition of anti-apoptotic gene expression like Bcl-2 was observed after 24h. An inhibitory concentration (IC50) of 220.01 µg/mL was obtained for the ethyl acetate extract. When observed under fluorescence microscope, stained cells showed an orange red color in the nuclei, indicating the ethyl acetate extract had induced apoptosis potentially for 24 h exposure cells expression, when compared with control cells. The results show that the ethyl acetate extracts were efficient in inhibiting the A549 cell line under in vitro condition, suggesting this extract can be further used as a potential chemotherapy drug.