The nitrates in pot cultures of gray wooded soil were generally affected by the various amendments as follows: increased by applications of neutral or acid subsurface peat and not depressed by neutral or acid surface peat; increased by ammonium phosphate alone and in combination with the surface and subsurface peats, and by ammonium sulphate in combination with the subsurface peats, but slightly depressed by triple superphosphate in combination with the subsurface peats.The wheat crop yields in pot cultures of gray wooded soil were generally affected by the various amendments as follows: increased somewhat by peat alone, and especially by subsurface peat; increased substantially by ammonium phosphate alone, but increased to a greater extent in all three series by a combination of ammonium phosphate and subsurface peat; increased substantially by ammonium sulphate and subsurface peat, but not by triple superphosphate and peat.The plate counts of bacteria in treated cultures of gray wooded soil increased for two to four months, and then decreased. The ammonium phosphate plus subsurface peat treatment gave the highest counts, the ammonium phosphate plus surface peat the second highest, and the ammonium sulphate plus subsurface peat the third highest. The differences between the effects of triple superphosphate plus subsurface peat, ammonium phosphate, surface peat, and subsurface peat, were small. The control gave only a small increase during the course of the experiment.The ratio method showed that there are very large numbers of bacteria in gray wooded soil. Instead of ranging from about 500,000 to about 8,000,000 per gm. of soil as with the plate count method, the numbers varied from about 200,000,000 to 2,000,000,000. The numbers reached a maximum after three months' incubation. The ammonium phosphate plus subsurface peat treatment again gave the highest average number, and the succeeding order was also about the same as in the case of the plate counts.When mixed with gray wooded soil, surface peat, though highly carbonaceous, did not cause any significant decrease in nitrate at first, and produced some increase later. Straw caused some decrease at first, but the later recovery counterbalanced this loss. Cotton caused a decrease to practically none for about three months, and even when combined with ammonium phosphate caused some decrease. Ammonium phosphate alone and in combination with straw and surface peat produced large increases in nitrate. In a duplicate experiment with black soil the trends were similar, but the nitrate was not reduced to the same extent by straw and cotton, and when ammonium phosphate was added with the organic matter, nitrate was not reduced by peat, straw, or cotton.