Effects Of Treadmill Training Research Articles

Effects of interval treadmill training on spatiotemporal parameters in children with cerebral palsy: A machine learning approach

Quantifying individualized rehabilitation responses and optimizing therapy for each person is challenging. For interventions like treadmill training, there are multiple parameters, such as speed or incline, that can be adjusted throughout sessions. This study evaluates if causal modeling and Bayesian Additive Regression Trees (BART) can be used to accurately track the direct effects of treadmill training on gait. We developed a Directed Acyclic Graph (DAG) to specify the assumed relationship between training input parameters and spatiotemporal outcomes during Short Burst Locomotor Treadmill Training (SBLTT), a therapy designed specifically for children with cerebral palsy (CP). We evaluated outcomes after 24 sessions of SBLTT for simulated datasets of 150 virtual participants and experimental data from four children with CP, ages 4–13 years old. Individual BART models were created from treadmill data of each step. Simulated datasets demonstrated that BART could accurately identify specified responses to training, including strong correlations for step length progression (R2 = 0.73) and plateaus (R2 = 0.87). Model fit was stronger for participants with less step-to-step variability but did not impact model accuracy. For experimental data, participants’ step lengths increased by 26 ± 13 % after 24 sessions. Using BART to control for speed or incline, we found that step length increased for three participants (direct effect: 13.5 ± 4.5 %), while one participant decreased step length (−11.6 %). SBLTT had minimal effects on step length asymmetry and step width. Tools such as BART can leverage step-by-step data collected during training for researchers and clinicians to monitor progression, optimize rehabilitation protocols, and inform the causal mechanisms driving individual responses.

The Effects of Treadmill Training with Visual Feedback and Rhythmic Auditory Cue on Gait and Balance in Cerebral Palsy: A Randomized Controlled Trial

The Effects of Treadmill Training with Visual Feedback and Rhythmic Auditory Cue on Gait and Balance in Cerebral Palsy: A Randomized Controlled Trial

Effects of treadmill training combined with transcranial direct current stimulation on mobility, motor performance, balance function, and other brain-related outcomes in stroke survivors: a systematic review and meta-analysis.

Treadmill training (TT) is a gait training technique that has commonly been used in neurorehabilitation, and has positive effects on gait, mobility, and related outcomes in stroke survivors. Transcranial direct current stimulation (tDCS) is a non-invasive approach for modulating brain cortex excitability. To evaluate the available scientific evidence on the effects of TT combined with tDCS on mobility, motor performance, balance function, and brain-related outcomes in stroke survivors. Five databases namely the Cochrane library, PEDro, Web of Science, PubMed, and EMBASE, were searched for relevant studies from inception to March, 2024. Only randomized controlled trials were included, and their methodological quality and risk of bias (ROB) were evaluated using the PEDro scale and Cochrane ROB assessment tool respectively. Qualitative and quantitative syntheses (using fixed effects meta-analysis) were employed to analyze the data. The results revealed that TT combined with active tDCS had significant beneficial effects on some mobility parameters, some gait spatiotemporal parameters, some gait kinematic parameters, gait endurance, gait ability, and corticomotor excitability in stroke survivors, but no significant difference on gait speed (P > 0.05), functional mobility (P > 0.05), motor performance (P > 0.05), or some balance functions (P > 0.05), compared with the control conditions. TT combined with active tDCS significantly improves some gait/mobility outcomes and corticomotor excitability in stroke survivors.

Treadmill exercise training inhibits morphine CPP by reversing morphine effects on GABA neurotransmission in D2-MSNs of the accumbens-pallidal pathway in male mice.

Relapse is a major challenge in the treatment of drug addiction, and exercise has been shown to decrease relapse to drug seeking in animal models. However, the neural circuitry mechanisms by which exercise inhibits morphine relapse remain unclear. In this study, we report that 4-week treadmill training prevented morphine conditioned place preference (CPP) expression during abstinence by acting through the nucleus accumbens (NAc)-ventral pallidum (VP) pathway. We found that neuronal excitability was reduced in D2-dopamine receptor-expressing medium spiny neurons (D2-MSNs) following repeated exposure to morphine and forced abstinence. Enhancing the excitability of NAc D2-MSNs via treadmill training decreased the expression of morphine CPP. We also found that the effects of treadmill training were mediated by decreasing enkephalin levels and that restoring opioid modulation of GABA neurotransmission in the VP, which increased neurotransmitter release from NAc D2-MSNs to VP, decreased morphine CPP. Our findings suggest the inhibitory effect of exercise on morphine CPP is mediated by reversing morphine-induced neuroadaptations in the NAc-to-VP pathway.

A systematic review evaluating the efficacy of treadmill training in geriatric care as an intervention for improving balance and reducing fall risks in elderly population

Background & Purpose Falls and balance issues are significant concerns for the elderly. Treadmill training is increasingly recognized as a potential intervention to improve balance and reduce fall risk in this population. This systematic review evaluates the effectiveness of treadmill training on balance in the elderly. Methods A comprehensive search was conducted in databases including MEDLINE, EMBASE, CINAHL Plus, PEDro, Cochrane Library, and ERIC from January 1, 1980, to May 31, 2023. The search focused on treadmill training’s impact on balance in older adults. From 74 identified studies, outcome measures were categorized into groups like “Balance Improvement,” “Gait Improvement,” “Mobility Enhancement,” “Muscle Strength Improvement,” “Cognitive Function and Quality of Life,” and others. Articles were excluded for reasons like irrelevance to treadmill training, language barriers, or duplication, resulting in 16 final studies. Results Treadmill training shows diverse positive effects on the elderly. Perturbation-based training reduces falls, and treadmill walking enhances balance and quality of life, particularly in institutionalized older individuals. Benefits were noted for Parkinson’s patients’ gait, cognitive changes in neurophysiology, fitness and mobility improvements through underwater treadmill sessions, and refined gait in hemiparetic patients. Conclusion Treadmill training interventions are promising for improving balance and mobility in the elderly, including those with Parkinson’s disease, spinal cord injuries, and age-related balance issues. While these interventions show potential, further research is needed to determine optimal protocols, durations, and combinations with other interventions. This could lead to a more comprehensive approach for addressing balance and mobility challenges in the elderly, promoting healthy aging and reducing fall risks.

Effects of Treadmill Training on Gross Motor Function, Spasticity and Gait Speed in Ambulatory Children with Spastic Cerebral Palsy

Background: Cerebral palsy is described as a permanent, non-progressive disorder related to neurodevelopmental abnormalities in an underdeveloped brain which leads to additional brain lesions resulting in secondary motor disturbances and postural abnormalities Objective: To determine the effectiveness of treadmill training on gross motor function, spasticity and gait speed in ambulatory children with spastic cerebral palsy. Methodology: It was a Randomized clinical trial, which was done in Bahria International Hospital, Lahore by convenient sampling technique. A sample size of 50 patients were divided into two groups. Patients in Group A underwent conventional physiotherapy, whereas those in Group B received a specialized treadmill training program tailored for children with spastic cerebral palsy, alongside their standard physical therapy sessions. Result: Our data is normally distributed so wo applied Independent sample t test for between group analysis which shows that mean of group A at baseline is 5.40±0.49 and group B is 5.20±0.76, at 6th weeks group A is 4.80±0.48 and group B is 4.33±.0.88and at 12th weeks group A is 3.93±0.58 and group B is 2.13±0.68.p-value shows that there is no significant difference between two groups at baseline and at 6th weeks but at 12th weeks there is a significant differences as p-value is less than 0.00.Repeated measure ANOVA shows both groups shows significant results but mean difference of treadmill training group were more in all outcome. Conclusion: - Our study concludes that treadmill training, along with physiotherapy, is beneficial for children with spastic cerebral palsy in improving gross motor function, spasticity, and gait speed.

The Impact of Treadmill Training on Tissue Integrity, Axon Growth, and Astrocyte Modulation.

Spinal cord injury (SCI) presents a complex challenge in neurorehabilitation, demanding innovative therapeutic strategies to facilitate functional recovery. This study investigates the effects of treadmill training on SCI recovery, emphasizing motor function enhancement, neural tissue preservation, and axonal growth. Our research, conducted on a rat model, demonstrates that controlled treadmill exercises significantly improve motor functions post-SCI, as evidenced by improved scores on the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale and enhanced electromyography readings. Notably, the training facilitates the preservation of spinal cord tissue, effectively reducing secondary damage and promoting the maintenance of neural fibers in the injured area. A key finding is the significant stimulation of axonal growth around the injury epicenter in trained rats, marked by increased growth-associated protein 43 (GAP43) expression. Despite these advancements, the study notes a limited impact of treadmill training on motoneuron adaptation and highlights minimal changes in the astrocyte and neuron-glial antigen 2 (NG2) response. This suggests that, while treadmill training is instrumental in functional improvements post-SCI, its influence on certain neural cell types and glial populations is constrained.

Effects of Tredmil Training with and without Mirror Therapy on Lower Limb Function, Dynamic Balance, and Gait in Chronic Stroke Patients

Stroke, a brain attack, causes approximately 17 million new strokes annually, leading to motor deficits in up to 80% of patients, 30% experiencing long-term deficits in independent walking, and two-thirds experiencing functional limitations in lower limbs. The objective of this research is to determine the effect of treadmill training with and without mirror therapy on lower limb function, dynamic balance, and gait in chronic stroke patients. This research involved 24 chronic stroke patients divided into two groups: Group A received lower-limb treadmill training with mirror treatment, and Group B received treadmill training without mirror treatment. Both groups received traditional physiotherapy methods. The study found that MAS was non-parametric, while other measures were parametric. The trial lasted eight weeks, using six-minute walk testing, the modified Ashworth scale, and Fugl-Meyer assessment (lower extremity). The study found that the "mirror therapy" group had better results than the "control group" group, with P values of 0.023 and 0.00, respectively, for the outcome measures "MAS post-intervention" and "6MWT post-intervention." The "Mirror Therapy" group also improved more than the "Control Group" group in the outcome measure "FMA-LE Post-Intervention."This study concluded that there was a significant association between treadmill training and mirror therapy and lower limb functioning, dynamic balance, and gait in chronic stroke patients.

Effects of Treadmill Training with Knowledge of Result on Balance in Stroke Patients

Effects of Treadmill Training with Knowledge of Result on Balance in Stroke Patients

Effect of treadmill training combined with ankle weight on balance in spastic cerebral palsied children: A randomized controlled trial

The aim of this study was to examine the impact of treadmill training combined with ankle weights on balance and dorsiflexor muscle strength in children with spastic hemiparetic cerebral palsy (CP). This clinical, randomized trial included forty boys and girls suffering from spastic hemiparetic cerebral palsy, ranging in age from 6 to 9 years old. They were split into two equal groups (control group = 20, study group = 20). Both groups received a program of physiotherapy in addition to treadmill exercise, while the study group also received ankle weights for eight weeks. The Biodex stability system was employed to evaluate stability indices, including the overall stability index (OASI), anteroposterior stability index (APSI), and mediolateral stability index (MLSI). Functional balance was measured using the Pediatric Balance Scale (PBS). A Lafayette manual muscle tester (MMT) was used to record the peak force of ankle dorsiflexor muscle strength for both groups, pre- and post-therapy. Post-therapy results showed a significant decrease in all stability indices at stability levels 8 and 4, and a significant increase was observed in functional balance and ankle dorsiflexor muscle strength in both groups (p<0.05). The study group showed a more significant improvement in all outcome measures after therapy than the control group (p<0.05). The program of physiotherapy, treadmill training, and ankle weights was effective in improving balance and dorsiflexor muscle strength among children with spastic hemiparetic cerebral palsy, with ankle weights contributing to greater progress.

Inhibition of CX3CL1 by treadmill training prevents osteoclast-induced fibrocartilage complex resorption during TBI healing.

The healing of tendon-bone injuries is very difficult, often resulting in poor biomechanical performance and unsatisfactory functional recovery. The tendon-bone insertion has a complex four distinct layers structure, and previous studies have often focused on promoting the regeneration of the fibrocartilage layer, neglecting the role of its bone end repair in tendon-bone healing. This study focuses on the role of treadmill training in promoting bone regeneration at the tendon-bone insertion and its related mechanisms. After establishing the tendon-bone insertion injury model, the effect of treadmill training on tendon-bone healing was verified by Micro CT and HE staining; then the effect of CX3CL1 on osteoclast differentiation was verified by TRAP staining and cell culture; and finally the functional recovery of the mice was verified by biomechanical testing and behavioral test. Treadmill training suppresses the secretion of CX3CL1 and inhibits the differentiation of local osteoclasts after tendon-bone injury, ultimately reducing osteolysis and promoting tendon bone healing. Our research has found the interaction between treadmill training and the CX3CL1-C3CR1 axis, providing a certain theoretical basis for rehabilitation training.

Effects of treadmill training on myelin proteomic markers and cerebellum morphology in a rat model of cuprizone-induced toxic demyelination

BackgroundMultiple sclerosis (MS) is the most common demyelinating disease of the central nervous system (CNS). If demyelination is persistent, it will result in irreversible axonal injury and loss. The purpose of the current study was to investigate the effects of treadmill training on myelin proteomic markers and cerebellum morphology in a rat model of cuprizone-induced toxic demyelination. MethodsThirty male rats were randomly assigned to five groups (n = 6 per group), consisting of a healthy control group (Control), a cuprizone (CPZ) group, and three exercise training groups: exercise training before and during the CPZ administration (EX-CPZ-EX), exercise training before the CPZ administration (EX-CPZ), and exercise training during the CPZ administration (CPZ-EX). A rat model of CPZ-induced toxic demyelination consisted of feeding the rats cuprizone pellets (0.2%) for 6 weeks. All exercise groups performed a treadmill training protocol 5 days/week for 6 weeks. Levels of Myelin proteolipid protein (PLP), Myelin oligodendrocyte glycoprotein (MOG), axonal injury in the cerebellar tissue, and volume, weight, and length of the cerebellum were determined. ResultsResults indicated a significant decrease in PLP and MOG in the CPZ groups compared to the Control group (****p < 0.0001). There was a significant increase in PLP and MOG and a significant decrease in axonal injury in the EX-CPZ-EX group as compared to other CPZ groups (****p < 0.0001), and CPZ-MS and CPZ-EX were not significantly different from one another. However, there were no significant differences between the groups for the volume, weight, or length of the cerebellum. ConclusionTreadmill training improved myelin sheath structural proteins and axonal injury in cerebellar tissue in a rat model of CPZ-induced toxic demyelination.

EFFECTS OF TREADMILL GAIT TRAINING ON BALANCE IN PARKINSON'S PATIENTS AFTER DEEP BRAIN STIMULATION.

After deep brain stimulation (DBS), patients with Parkinson's disease (PD) typically still present significant gait and postural stability problems, and thus additional interventions are needed. In this way, our purpose was evaluate the comparative effectiveness of treadmill training, with and without body weight support, on balance outcomes among patients with PD after DBS. Eleven patients with PD that were using bilateral subthalamic nucleus DBS were evaluated using Time Up and Go test (TUG); Berg Balance Scale (BBS) and Static Posturography. In phase 1, all subjects participated in 8-weeks of treadmill training in conjunction with conventional physiotherapy. After six weeks (wash-out), each patient then participated in a subsequent 8-weeks of treadmill training with partial body weight support. After the phase 1, there were improvements on the cognitive TUG performance (Before: 15.7 ± 1,8 sec; After: 13.7 ± 3.1 sec; p < 0.01) and an increase of anteroposterior and medio-lateral body oscillation with eyes closed. After the phase 2, there were improvements in conventional (Before: 12.3 ± 2.0 sec; After: 10.7 ± 1.7 sec; p < 0.01) and cognitive (Before: 14.6 ± 3.5 sec; After: 12.5 ± 1.6 sec; p < 0.05) TUG performances. There were no significant changes in the Berg Balance Scale following either training protocol. Both trainings improved static and dynamic balance and had similar results; however, supported treadmill training seemed to be a potentially superior option, as patients tended to feel safer. Level of Evidence II, therapeutic studies - investigation of treatment outcomes.

Postural control deficits due to bilateral pyramidal tract lesions exemplified by hereditary spastic paraplegia (HSP) originate from increased feedback time delay and reduced long-term error corrections.

Pyramidal tract lesions determine the clinical syndrome of Hereditary Spastic Paraplegia (HSP). The clinical impairments of HSP are typically exemplified by their deficits in mobility, leading to falls and injuries. The first aim of this study was to identify the cause for postural abnormalities caused by pyramidal tract lesions in HSP. The second aim was to specify the effect of treadmill training for postural abnormalities. We examined nine HSP patients before and after treadmill training, as well as nine healthy control subjects during perturbed and unperturbed stance. We found that HSP was associated with larger sway amplitudes and velocities. Body excursions following platform tilts were larger, and upper body excursions showed a phase lead. Model-based analysis detected a greater time delay and a reduced long-term error correction of postural reactions in the center of mass. HSP patients performed significantly better in clinical assessments after treadmill training. In addition, treadmill training reduced sway amplitudes and body excursions, most likely by increasing positional and velocity error correction gain as a compensatory mechanism, while the time delay and long-term error correction gain remained largely unaffected. Moreover, the upper body's phase lead was reduced. We conclude that HSP leads to very specific postural impairments. While postural control generally benefits from treadmill training, the effect seems to mainly rely on compensatory mechanisms, whereas the original deficits are not affected significantly.

Myelin repair of spinal cord injury in adult mice induced by treadmill training upregulated peroxisome proliferator-activated receptor gamma coactivator 1 alpha.

Growing evidence has proven the efficacy of physical exercise in remyelination and motor function performance after spinal cord injury (SCI). However, the molecular mechanisms of treadmill training on myelin repair and functional recovery after SCI have not yet been fully studied. Here, we explored the effect of treadmill training on upregulating peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α)-mediated myelin repair and functional recovery in a mouse model of thoracic T10 contusion injury. A 4-week treadmill training scheme was conducted on mice with SCI. The expression levels of oligodendrogenesis-related protein and PGC1α were detected by immunofluorescence, RNA fluorescence in situ hybridization and western blotting. Transmission electron microscopy (TEM) was used to observe myelin structure. The Basso Mouse Scale (BMS) and CatWalk automated gait analysis system were used for motor function recovery evaluation. Motor evoked potentials (MEPs) were also identified. In addition, adeno-associated virus (AAV)-mediated PGC1α knockdown in OLs was used to further unravel the role of PGC1α in exercise-induced remyelination. We found that treadmill training boosts oligodendrocyte precursor cells (OPCs) proliferation, potentiates oligodendrocytes (OLs) maturation, and increases myelin-related protein and myelin sheath thickness, thus impelling myelin repair and hindlimb functional performance as well as the speed and amplitude of nerve conduction after SCI. Additionally, downregulating PGC1α through AAV attenuated these positive effects of treadmill training. Collectively, our results suggest that treadmill training enhances remyelination and functional recovery by upregulating PGC1α, which should provide a step forward in the understanding of the effects of physical exercise on myelin repair.

Effectiveness of Treadmill Training Alone Versus Treadmill Training with External Clues on Improving Gait and Functional Ability in Parkinson’s Patient

Parkinson's disease is a progressive neurological disease caused by degeneration of nerve cells in Substantia Nigra. It is characterized by postural instability, slowing of movement and tremor. Treadmill Training with Visual clues extensively used as an intervention to improve gait and Functional mobility. Objective: To compare the Treadmill training alone versus treadmill training with visual clues on improve gait and functional ability in Parkinson’s disease. Methods: It is assessor blind randomized controlled trial conducted in CMA Research and Teaching hospital, Lahore from 6th June 2022 to 6th January 2023.The sample size of 74 were divided into two groups. Group A received treadmill training session for 30 minutes while. The Group B received treadmill training with external clues like visual, auditory, etc. Each Participant received total 56 sessions for 28 weeks. The outcome measures was functional Activity measures with Wisconsin gait scale for Gait and Motor Aspects of Experiences of Daily Living (M-EDL) questionnaire. The Data were assessing at baseline by using these two scales. Results: The between groups comparison of post value showed that Group B showed a significant (&lt;0.05) improvement in the (M-EDL) scale for the activities of daily living and significant (&lt;0.05) improvement in the step length, stance width, hip hiking, pelvic rotation, external rotation, and toe clearance. Conclusions: It was concluded that the treadmill training with external clues is better as compared to the treadmill training alone for improving the gait and functional ability in the Parkinson patient.

Exploring Potential Predictors of Treadmill Training Effects in People With Parkinson Disease

ObjectiveTo explore the potential predictors of people with Parkinson disease (PD) who would benefit the most from treadmill training. DesignA cohort study. SettingMedical university rehabilitation settings. ParticipantsSeventy participants diagnosed of idiopathic PD. InterventionsTwelve sessions of treadmill training. Main Outcome MeasuresHierarchical logistic regression models were used to explore significant predictors of the treadmill training effect with respect to 3 health domains: Unified Parkinson's Disease Rating Scales part III (UPDRS III); gait speed; Parkinson's Disease Questionnaire-39 (PDQ-39). A receiver operating characteristic (ROC) curve analysis was conducted to identify proper cut-off points for clinical use. ResultsMale sex (adjusted odds ratio [OR]: 3.73, P=.036) significantly predicted the improvement of UPDRS III. Individuals with a slower baseline gait speed (cut-off: 0.92 m/s, adjusted OR: 14.06, P<.001) and higher baseline balance confidence measured by the Activity-specific Balance Confidence scale (cut-off: 84.5 points, adjusted OR: 4.66, P=.022) have greater potential to achieve clinically relevant improvements in gait speed. A poorer baseline PDQ-39 score (cut-off: 23.1, adjusted OR: 7.47, P<.001) predicted a greater quality of life improvement after treadmill training. ConclusionsThese findings provide a guideline for clinicians to easily identify suitable candidates for treadmill training. Generalization to more advanced patients with PD warrants further investigation.

Effect Of Treadmill Training In A Rodent Model Of Autism Spectrum Disorder

Effect Of Treadmill Training In A Rodent Model Of Autism Spectrum Disorder

The effects of treadmill training on postural control and balance in children with spastic diplegic cerebral palsy: A cross-over controlled study

Background &amp; Objective: Treadmill training (TT) is used for several targets as in walking speed and endurance in rehabilitation programs of children with cerebral palsy (CP). However, its effects on postural stability have not been fully explored. The aim of this study was to investigate the effects of unsupported TT on postural control (PC) parameters and balance in children with spastic diplegic CP. Methods: Twelve children with CP, level I-II according to Gross-Motor-Function-Classification- System (GMFCS) were included. Participants were divided into two groups using randomized-sampling method. The study was designed as a cross-over study. In the first phase, the first group underwent routine physiotherapy-rehabilitation program (PTR) 3 sessions/week, 45 minutes per session, for 12 weeks. In the second group, 20 minutes of TT starting with 0.5 km/h speed, was added to the same PTR (n=6). At the end of 12 week, 4-week-long wash-out period was given. After this 4 weeks period, both groups crossed-over for another 12 weeks at the second phase of therapy. All tests was applied at baseline and at end of the first and second phases. PC was evaluated with Balance-Master computerized posturography (Neurocom Inc.) which consisted of tests of modified-clinical-sensory- balance-interaction (MCSBT), weight-shifting-in-standing (WSST), limits-of-stability (LoST) and rhythmic-weight-shifting (RWST). Results: The two groups were similar in age, body composition, GMFCS Levels and spasticity levels before the treatment and after the wash-period (p&gt;0,05). After TT, there were significant improvements in PC parameters, MCSBT: composite-balance-score (p=0.02), center of gravity alignment (p=0.02); WSST: symmetry (p=0.03); LoST: backward weight-shifting (p=0,02), end point reaching (p=0.02-0.04), maximum-orientation (p=0.02-0.04); RWST: direction- control (p=0.02-0.04), on-axis-velocity (p=0.02-0.04). Conclusion: Including TT in PTR treatment program can enhance PC and balance in children with CP.

Effect of treadmill training on fibrocartilage complex repair in tendon-bone insertion healing in the postinflammatory stage.

Mechanical stimulation is a key factor in the development and healing of tendon-bone insertion. Treadmill training is an important rehabilitation treatment. This study aims to investigate the benefits of treadmill training initiated on postoperative day 7 for tendon-bone insertion healing. A tendon-bone insertion injury healing model was established in 92 C57BL/6 male mice. All mice were divided into control and training groups by random digital table method. The control group mice had full free activity in the cage, and the training group mice started the treadmill training on postoperative day 7. The quality of tendon-bone insertion healing was evaluated by histology, immunohistochemistry, reverse transcription quantitative polymerase chain reaction, Western blotting, micro-CT, micro-MRI, open field tests, and CatWalk gait and biomechanical assessments. Our results showed a significantly higher tendon-bone insertion histomorphological score in the training group, and the messenger RNA and protein expression levels of type II collagen (COL2A1), SOX9, and type X collagen (COL10A1) were significantly elevated. Additionally, tendon-bone insertion resulted in less scar hyperplasia after treadmill training, the bone mineral density (BMD) and bone volume/tissue volume (BV/TV) were significantly improved, and the force required to induce failure became stronger in the training group. Functionally, the motor ability, limb stride length, and stride frequency of mice with tendon-bone insertion injuries were significantly improved in the training group compared with the control group. Treadmill training initiated on postoperative day 7 is beneficial to tendon-bone insertion healing, promoting biomechanical strength and motor function. Our findings are expected to guide clinical rehabilitation training programmes.