Effect Of Taurodeoxycholate Research Articles

Effect of taurodeoxycholate and taurocholate on the secretion of mucous glycoprotein (MGP) by porcine gallbladder epithelium

Effect of taurodeoxycholate and taurocholate on the secretion of mucous glycoprotein (MGP) by porcine gallbladder epithelium

Calcium Fluxes Caused by Bile Salts in Rat Pancreatic Acini

The effect of bile salt on Ca2+ uptake, Ca2+ efflux, and cytosolic free Ca2+ concentration ([Ca2+]i) in rat pancreatic acini has been studied. The dihydroxy bile salts, taurodeoxycholate (TDC) and taurochenodeoxycholate (TCDC), were found to accelerate Ca2+ uptake. Dihydroxy bile salt and the trihydroxy bile salt taurocholate (TC) stimulated Ca2+ efflux from the acini. Verapamil increased the Ca2+ efflux induced by dihydroxy bile salts but did not influence the Ca2+ uptake. Under calcium-equilibrated conditions TDC and TCDC caused a quick net Ca2+ efflux, followed by an increase in Ca2+ uptake, whereas TC only caused a net Ca2+ efflux. TDC and TCDC, but not TC, increased the [Ca2+]i. This effect of TDC and TCDC was abolished in Ca2+-free EDTA-containing (0.2 mM) medium. The amylase release caused by bile salts was related in time with the Ca2+ fluxes. In conclusion, bile salts may change the Ca2+ homeostasis of pancreatic acini in different ways, depending on the type of bile salt. This change of Ca2+ homeostasis may play an important role in the bile salt-stimulated amylase release.

Effects of taurodeoxycholate on in vivo water and solute transport in rat jejunum in absence and presence of calcium.

Bile acids and fatty acids enhance the permeability of brush-border membrane vesicles for calcium. It has been postulated that increased influx of calcium into the enterocyte might be responsible for the fluid secretion induced by dihydroxy bile acids and fatty acids. During in vivo perfusion studies of the rat jejunum, 15 mM taurodeoxycholate induced secretion of electrolytes and water (P less than 0.001), reduced glucose absorption (P less than 0.001), and enhanced the absorption of mannitol (P less than 0.0125) and calcium (P less than 0.001). Calcium absorption continued to be enhanced during perfusion of a CaCl2-containing solution following the perfusion with taurodeoxycholate (P less than 0.05). In view of the previously demonstrated enhanced permeability of the apical brush-border membrane in the presence of bile acids, it is very likely that some calcium enters the enterocyte along the steep concentration gradient in the presence of taurodeoxycholate. In spite of enhanced calcium absorption, 15 mM CaCl2 had no effect on control absorption rates or on fluid secretion induced by taurodeoxycholate. The data indicate that the effects of bile acids on intestinal transport are not mediated by an influx of calcium into the enterocyte.

Effect of taurodeoxycholate, colipase and temperature on the interfacial inactivation of porcine pancreatic lipase.

Beside their inhibitory effect upon lipase adsorption, bile salts at low concentration (around 0.2 mM) have repeatedly been shown to enhance lipolysis slightly. From the data reported in this paper, this activation has been attributed to a stabilization of the adsorbed lipase brought about by low concentrations of bile salts. This hypothesis relies on the following observations. (a) For a given temperature, the activation by bile salts depends on the substrate. It is maximum for trihexanoin (trihexanoylglycerol) and does not exist for tripropionin (tripropionylglycerol). (b) In the absence of bile salts, the optimal activities are obtained for different temperatures depending on the substrate. (c) For a given substrate, the activation by a low concentration of bile salts depends on the temperature. It increases when the temperature is raised (up to 35-40 degrees C) and completely disappears at a sufficiently low temperature (around 10 degrees C). (d) This temperature effect does not seem to be due to a modification of the physical parameters of the interface as measured by the interfacial tension. (r) Colipase, like bile salts, increases the lipase activity on short-chain triglycerides but only at high temperature when lipase denaturation occurs. It has no influence upon the activity when the temperature is sufficiently low (around 10 degrees C).

High-resolution proton magnetic resonance study of porcine colipase and its interactions with taurodeoxycholate.

A high-resolution 270-MHz proton NMR study of procine colipase I has been performed, and the resonances in the aromatic region of the spectrum have been assigned to amino acid residues by pH titration and decoupling experiments. The apparent pKa values of the three tyrosines were calculated to be 10.2, 10.3, and 11.8 with one of the tyrosines having properties of a "buried" residue. A tentative assignment to the amino acid residues in the primary seuqence of colipase will be discussed. The effects of taurodeoxycholate (TDC) and a positively charged deoxycholate derivative on the aromatic region of the colipase NMR spectrum indicate that all tyrosines and one histidine are affected by the bile-salt binding, suggesting that the TDC molecules bind near these residues to a hydrophobic region on colipase. Measurements and calculations on the line width of the C(18) methyl group resonance suggest that the line-width increase of this resonance upon interaction of TDC with colipase to a large extent can be explained as due to the slower tumbling of the TDC molecules bound to colipase.

Acid pH enhances the effect of taurodeoxycholate on water transport in the human jejunum

Acid pH enhances the effect of taurodeoxycholate on water transport in the human jejunum