Effects Of Stimulus Preexposure Research Articles

Supplemental Material for The Effects of Stimulus Pre-Exposure and Conditioning on Overt Visual Attention

Supplemental Material for The Effects of Stimulus Pre-Exposure and Conditioning on Overt Visual Attention

Response reduction and stimulus pre-exposure effects in a human conditioning method.

Two experiments with humans determined whether reduced conditioning following pre-exposure to the conditioned stimulus could be explained by conditioned inhibition (Experiment 1 [E1]) or extinction of responding that the conditioned stimulus (CS) might elicit during pre-exposure (Experiment 2 [E2]). In a video game task (Nelson et al., 2014), participants learned to respond to lights that signaled attacking spaceships. In E1, a red light was either pre-exposed or not pre-exposed between groups prior to conditioning with a green light. Summation tests of red combined with green produced no evidence of conditioned inhibition. In E2, participants received either no pre-exposure to the light, exposure in the same context in which the conditioning would occur, or exposure in a different context. These conditions were factorially combined with whether the light and spaceship were similar (same color) or dissimilar (different colors). In the similar conditions, the light elicited weak responding during pre-exposure, which extinguished. Such extinction did not occur in the dissimilar conditions. Conditioning occurred more rapidly in the similar conditions than in the dissimilar ones, but both conditions showed an equivalent context-dependent pre-exposure effect. Pre-exposure reduced conditioning regardless of whether it reduced responding prior to conditioning. The data are consistent with animal research (Lubow et al., 1968) showing no relation between responding during pre-exposure and the effects of stimulus pre-exposure. Theories which account for the effects of stimulus pre-exposure are discussed, with the conclusion that the data are most consistent with the ideas presented by Wagner (1981). (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Anterior thalamic nuclei, but not retrosplenial cortex, lesions abolish latent inhibition in rats.

The present study examined the effects of excitotoxic lesions in 2 closely related structures, the anterior thalamic nuclei and the retrosplenial cortex, on latent inhibition. Latent inhibition occurs when nonreinforced preexposure to a stimulus retards the subsequent acquisition of conditioned responding to that stimulus. Latent inhibition was assessed in a within-subject procedure with auditory stimuli and food reinforcement. As expected, sham-operated animals were slower to acquire conditioned responding to a stimulus that had previously been experienced without consequence, relative to a non-preexposed stimulus. This latent inhibition effect was absent in rats with excitotoxic lesions in the anterior thalamic nuclei, as these animals conditioned to both stimuli at equivalent rates. The retrosplenial lesions appeared to spare latent inhibition, as these animals displayed a robust stimulus preexposure effect. The demonstration here that anterior thalamic nuclei lesions abolish latent inhibition is consistent with emerging evidence of the importance of these thalamic nuclei for attentional control.

The Hall-Rodriguez theory of latent inhibition: Further assessment of compound stimulus preexposure effects.

According to a recent theory (Hall & Rodriguez, 2010), the latent inhibition produced by nonreinforced exposure to a target stimulus (B) will be deepened by subsequent exposure of that stimulus in compound with another (AB). This effect of compound exposure is taken to depend on the addition of a novel A to the familiar B and is not predicted for equivalent preexposure on which AB trials precede the A trials. This prediction was tested in 2 experiments using rats. Experiment 1 used an aversive procedure with flavors as the stimuli; Experiment 2 used an appetitive procedure with visual and auditory stimuli. In both, we found that conditioning with B as the conditioned stimulus proceeded more slowly (i.e., latent inhibition was greater) in subjects given the B-AB sequence in preexposure than in subjects given the AB-B sequence.

The unconditioned stimulus pre‐exposure effect in preweanling rats in taste aversion learning: Role of the training context and injection cues

The unconditioned stimulus pre-exposure effect (US-PE) refers to the interference paradigm in which acquisition of the conditioned response is retarded due to prior experience with the US. Most studies analyzing the psychological mechanisms underlying this effect have been conducted with adult rats. The most widely accepted hypothesis explains this effect as a contextual blocking effect. Contextual cues associated with the US block the conditioned stimulus (CS)-US association during conditioning. The modulatory role of a context devoid of distinctive olfactory attributes is not observable until approximately PD23 in rats, including modulation of interference paradigms such as latent inhibition or extinction. In this study, we analyzed US-PE in preweanling rats along with the role of the training context in this effect in terms of conditioned taste aversion preparation. Pre-exposure to LiCl before conditioning retarded the acquisition of taste aversion. The US-PE was observed in preweanling rats when, during pre-exposure, subjects were exposed to the conditioning context, and this effect was not attenuated either by the administration of the US in a familiar environment (Experiment 1a), or by the presence of an alternative, more salient context during pre-exposure (Experiment 1b). Additionally, the US-PE was still observed when the route by which the US was administered was changed between the pre-exposure and conditioning phases (Experiment 2a) as well as when the injection cues were removed during conditioning (Experiment 2b). These experiments show a strong US-PE in preweanling rats and fail to support the contextual blocking hypothesis, at least in this stage of ontogeny.

Positive and negative affect produce opposing task-irrelevant stimulus preexposure effects.

In three experiments, groups were exposed to either positive or negative affect video clips, after which they were presented with a series of task-irrelevant stimuli. In the subsequent test task, subjects were required to learn an association between the previously irrelevant stimulus and a consequence, and between a new stimulus and a consequence. Induced positive affect produced a latent inhibition effect (poorer evidence of learning with the previously irrelevant stimulus than with the novel stimulus). In opposition to this, induced negative affect resulted in better evidence of learning with a previously irrelevant stimulus than with a novel stimulus. In general, the opposing effects also were present in participants scoring high on self-report questionnaires of depression (Experiments 2 and 3). These unique findings were predicted and accounted for on the basis of two principles: (a) positive affect broadens the attentional field and negative affect contracts it; and (b) task-irrelevant stimuli are processed in two successive stages, the first encodes stimulus properties, and the second encodes stimulus relationships. The opposing influences of negative and positive mood on the processing of irrelevant stimuli have implications for the role of emotion in general theories of cognition, and possibly for resolving some of the inconsistent findings in research with schizophrenia patients.

Enhanced conditioned eyeblink response acquisition and proactive interference in anxiety vulnerable individuals.

In classical conditioning, proactive interference may arise from experience with the conditioned stimulus (CS), the unconditional stimulus (US), or both, prior to their paired presentations. Interest in the application of proactive interference has extended to clinical populations as either a risk factor for disorders or as a secondary sign. Although the current literature is dense with comparisons of stimulus pre-exposure effects in animals, such comparisons are lacking in human subjects. As such, interpretation of proactive interference over studies as well as its generalization and utility in clinical research is limited. The present study was designed to assess eyeblink response acquisition after equal numbers of CS, US, and explicitly unpaired CS and US pre-exposures, as well as to evaluate how anxiety vulnerability might modulate proactive interference. In the current study, anxiety vulnerability was assessed using the State/Trait Anxiety Inventories as well as the adult and retrospective measures of behavioral inhibition (AMBI and RMBI, respectively). Participants were exposed to 1 of 4 possible pre-exposure contingencies: 30 CS, 30 US, 30 CS, and 30 US explicitly unpaired pre-exposures, or Context pre-exposure, immediately prior to standard delay training. Robust proactive interference was evident in all pre-exposure groups relative to Context pre-exposure, independent of anxiety classification, with CR acquisition attenuated at similar rates. In addition, trait anxious individuals were found to have enhanced overall acquisition as well as greater proactive interference relative to non-vulnerable individuals. The findings suggest that anxiety vulnerable individuals learn implicit associations faster, an effect which persists after the introduction of new stimulus contingencies. This effect is not due to enhanced sensitivity to the US. Such differences would have implications for the development of anxiety psychopathology within a learning framework.

Signaling the unconditioned stimulus during the preexposure phase does not attenuate the unconditioned stimulus preexposure effect in preweanling rats

The unconditioned stimulus preexposure effect (US-PE) is defined as an attenuation of the conditioned response after preexposure to the US prior to conditioning. Evidence exists that this effect can be weakened or eliminated by the presence of a signal predicting the US during the preexposure phase. This evidence has been found consistently across a variety of procedures in adult rats. The aim of the present study was to evaluate whether, in infant rats, signaling the US (LiCl) during preexposure with a salient cue (almond odor) attenuates the US-PE. During the preexposure phase, preweanling rats received three (Experiment 1) or one (Experiment 2) preexposures to LiCl, preceded by exposure to almond odor. Appropriate control groups were also included in these experiments. After preexposure, two conditioning trials were carried out in which subjects were given LiCl after saccharin consumption. During preexposure, three (Experiment 1a), although not one (Experiment 2a), contingent exposures to almond odor and LiCl resulted in a strong odor aversion. Extinction of the learned taste aversion was facilitated by prior experience with LiCl (Experiments 1b and 2b). This effect was observed regardless of whether or not LiCl was signaled by the almond odor. These results do not coincide with the associative hypotheses proposed to explain the US-PE, nor are they concurrent with alternative explanations based on the learned helplessness phenomenon.

Reinforced stimulus preexposure effects as a function of US intensity: Implications for understanding the Hall–Pearce effect

Three conditioned suppression experiments examined the Hall–Pearce (1979) negative transfer effect in rats. Experiment 1 replicated the effect: CS-US weak pairings retarded subsequent fear conditioning to the CS as a result of CS-US strong pairings. The size of this retardation was less than that produced by non-reinforced CS presentations (latent inhibition). When the magnitude of the US weak was reduced in Experiment 2, the Hall–Pearce effect was greater than latent inhibition. Experiment 3 confirmed the findings of the two previous experiments, and demonstrated that magnitude of the Hall–Pearce negative transfer effect is inversely related to the magnitude of the US weak. From these findings it is suggested that the Hall–Pearce effect consists of a balance between a positive transfer of associative strength, and negative transfer based on CS- and US-preexposure effects.

Latent inhibition in within-subject designs: The roles of masking, schizotypy, and gender

Latent inhibition (LI) is defined as poorer associative learning with a previously exposed, irrelevant stimulus than with a non-preexposed, novel stimulus. This study examined how type of within-subject procedure, class of schizotypal symptoms, and gender modulate LI. Two within-subject procedures were examined, one excluding, and one including a masking task. The former yielded a stronger stimulus preexposure effect than the latter. However, the stimulus preexposure effect was attenuated by total schizotypy score in the masked, but not in the non-masked procedure. The results with schizotypy factors (positive and negative symptoms) as well as with schizotypy factors × gender interactions were inconsistent with those of other studies. It was suggested that in addition to LI, interference and novel pop-out effects were also produced by the procedures, particularly in the non-masked condition.

Disruption of the US pre-exposure effect and latent inhibition in two-way active avoidance by systemic amphetamine in C57BL/6 mice

Pre-exposure to either one of the two to-be-associated stimuli alone is known to reduce the efficiency of the learning of their association when they are subsequently paired explicitly. In classical conditioning, pre-exposure to the conditioned stimulus (CS) gives rise to latent inhibition (LI); and pre-exposure to the unconditioned stimulus (US) results in the US pre-exposure effect (USPEE). Considerable evidence supports an important role of central dopamine in the regulation and modulation of LI; it has been suggested that the USPEE may be similarly controlled by dopamine, but this parallelism has only been directly demonstrated in the conditioned taste aversion paradigm. The present study tested this hypothesis by comparing the efficacy of systemic amphetamine treatment to affect the expression of LI and the USPEE in a two-way active avoidance paradigm. C57BL/6 male mice were tested in active avoidance using a tone CS and a foot-shock US. Twenty-four hours before, they were pre-exposed to 100 presentations of the CS or the US, or to the test apparatus only. Amphetamine (2.5 mg/kg) or saline was administered before stimulus pre-exposure and conditioned avoidance test, in which the mice learned to avoid the shock by shuttling in response to the tone. Amphetamine disrupted both stimulus pre-exposure effects, thus, lending further support to the hypothesis that the USPEE is similar to LI in its sensitivity to dopamine receptor agonist. Hence, the USPEE paradigm may represent a valuable addition to the study of dopamine-sensitive processes of selective learning currently implicated in LI and Kamin blocking.

Transfer of configural learning between the components of a preexposed stimulus compound: Implications for elemental and configural models of learning.

In 2 experiments, rats received preexposure to 2 compound contexts: AB and CD for the congruent group and AC and BD for the incongruent group. Subsequently, all rats received a configural discrimination in which separate placement in contexts A or B indicated that presentations of stimulus X would be followed by food and presentations of Y would not, and separate placement in contexts C and D indicated that Y would be followed by food and X would not. In both experiments, rats in the congruent group acquired the conditional discrimination more rapidly than those in the incongruent group. These results are inconsistent with conventional associative accounts of either stimulus preexposure effects or configural learning and instead provide support for a connectionist account.

Latent inhibition is disrupted by nucleus accumbens shell lesion but is abnormally persistent following entire nucleus accumbens lesion: The neural site controlling the expression and disruption of the stimulus preexposure effect

Latent inhibition (LI) is the proactive interference of repeated nonreinforced preexposure to a stimulus with subsequent performance on a learning task involving that stimulus. The present experiments investigated the role of the nucleus accumbens (NAC) in LI. LI was measured in a thirst motivated conditioned emotional response procedure with low or high number of conditioning trials, and in two-way active avoidance procedure with the stages of preexposure and conditioning taking place in the same or different contexts. Sham-lesioned rats showed LI with low but not high number of conditioning trials and if preexposure and conditioning took place in the same context but not if the context was changed between the stages. Lesion to the shell subregion of the NAC disrupted LI but LI was preserved in rats with a combined lesion to the NAC shell and core subregions. Moreover, rats with a combined shell-core lesion persisted in showing LI in spite of high number of conditioning trials and in spite of context change. These results show that the NAC is not essential for the acquisition of LI but rather plays a key role in regulating the expression of LI. Moreover, they suggest that the two subregions of the NAC contribute competitively and cooperatively to this process, selecting the response appropriate to the stimulus-no event or the stimulus-reinforcement association in conditioning.

Delay-Induced Super-Latent Inhibition as a Function of Order of Exposure to Two Flavours Prior to Compound Conditioning

A number of recent conditioned taste aversion (CTA) experiments have demonstrated a super-latent inhibition (LI) effect--namely, a time-induced increase in the effects of stimulus preexposure when the interval between acquisition and test is spent in a context that is different from the other experimental contexts. Two CTA experiments with rats were conducted to examine the role of primacy in producing super-LI. In Experiment 1, one of two flavours was pre-exposed, following which a second flavour was preexposed. After the second preexposure, animals were conditioned by pairing a compound of the two preexposed flavours with LiCl. The test stage was conducted 1 or 21 days after conditioning, with the interval being spent in either the same or different contexts. In the test, animals were confronted with two bottles, each with one of the two preexposed flavours. Super-LI was obtained only for the first preexposed flavour in the 21-day delay group that spent the interval in a different context. Experiment 2 was designed to ensure that the effects in Experiment 1 represented LI, and to control for order of presentation of the flavours and time between preexposure and acquisition. The results replicated those of Experiment 1. The two experiments support the importance of primacy in the general super-LI experiment where CS-alone preexposure precedes CS-US.

Lesions to the basolateral amygdala and the orbitofrontal cortex but not to the medial prefrontal cortex produce an abnormally persistent latent inhibition in rats

Repeated nonreinforced preexposure to a stimulus interferes with the establishment of conditioned responding to this stimulus when it is subsequently paired with reinforcement. This stimulus–preexposure effect is known as latent inhibition (LI). Rather remarkably, LI appears to be resistant to the effects of numerous lesions, including the prefrontal cortex (PFC) and the basolateral amygdala (BLA). However, intact behavioral expression of LI following damage to given brain regions does not preclude the possibility that such regions participate in the regulation of LI expression in the intact brain. The present study showed that lesions of the BLA and the orbitofrontal cortex (OFC) but not of the medial PFC (mPFC) led to an abnormally persistent LI which emerged under conditions that disrupted LI in control rats. LI was measured in a thirst motivated conditioned emotional response procedure by comparing suppression of drinking in response to a tone in rats which received 0 (nonpreexposed) or 40 tone presentations (preexposed) followed by either two or five tone-shock pairings. Control rats showed LI with 40 preexposures and two conditioning trials, but raising the number of conditioning trials to five disrupted LI. OFC- and BLA-lesioned rats showed LI under the former condition but in addition persisted in exhibiting LI under the latter condition. Rats with lesion of the mPFC did not show persistent LI. Thus, although LI does not depend on the integrity of BLA and OFC (because it is present in BLA- and OFC- lesioned rats even under conditions disrupting the phenomenon in normal rats), these regions play an important role in the modulation of its expression, more specifically, in the control of the non-expression of LI when the impact of conditioning increases beyond a certain level.

Latent inhibition is spared by n-methyl- d-aspartate (nmda)-induced ventral hippocampal lesions, but is attenuated following local activation of the ventral hippocampus by intracerebral nmda infusion

Repeated non-reinforced exposures of a neutral stimulus retard the development of a conditioned response to that stimulus when it is subsequently paired with a significant event. This stimulus pre-exposure effect is known as latent inhibition (LI). Early lesion studies have initially suggested an important role for the hippocampus in the normal development and expression of LI. This view has since been modified with the emergence of data derived from selective cell body lesions of the hippocampus and of the entorhinal cortex, with an abolition of LI only seen after lesions of the latter. This suggests that the significance of the hippocampus might have been overestimated in the past, possibly due to interruption of fibres en passage. However, intact behavioural expression of LI following hippocampal damage does not preclude the suggestion that the hippocampus participates in the control and regulation of LI expression in intact animals. The present study demonstrated that whilst cell body lesions of the ventral hippocampus spared LI (as expected), chemical activation of the ventral hippocampus by local N-methyl- d-aspartate infusion disrupted LI. These results parallel our earlier observations on prepulse inhibition (PPI) with similar manipulations [Neuroreport 10 (1999) 2533]. Thus, although the ventral hippocampus is itself not responsible for the behavioural manifestation of LI and PPI, it exerts at least a modulatory control over the form and/or magnitude of their expression. Our results should prompt a re-evaluation of the relative roles of the hippocampus and retrohippocampus in the development and expression of LI.

Repeated administration of LiCl produces an unconditioned stimulus preexposure effect in backward excitatory CTA but not habituation of the unconditioned increment in neophobia

In one experiment using conditioned taste aversion and the unconditioned stimulus (US) preexposure procedure, one group of rats was given LiCl exposure for 3 days, whereas two other groups received saline. Following this phase, all groups were given a novel flavour (saccharine) to drink following either LiCl (group preexposed and one of the control groups) or saline injections (the remaining control group) and the consumption of the flavour was assessed. After this neophobia test, the acquired saccharine aversion was evaluated. The results show that three LiCl injections are enough to produce a US preexposure effect on backward excitatory taste aversion conditioning, whereas this number of injections procedure does not produce habituation of the increment in neophobia, an unconditioned response to the LiCl. The results are discussed taking into account different mechanisms involved in US preexposure effect.

Pre-exposure to wheel running disrupts taste aversion conditioning

When rats are given access to a running wheel after drinking a flavored solution, they subsequently drink less of that flavor solution. It has been suggested that running produces a conditioned taste aversion (CTA). This study explored whether CTA is eliminated by prior exposure to wheel running [i.e., unconditioned stimulus (UCS) pre-exposure effect]. The rats in the experimental group (UW) were allowed to wheel run for 1 h daily for seven consecutive days of pre-exposure. Rats in the two other groups had either access to locked wheels (LW group) or were maintained in their home cages (HC group) during the pre-exposure days. All rats were then exposed to four paired and four unpaired trials using a “ABBAABBA” design. Conditioning trials were composed of one flavored liquid followed by 60-min access to wheel running. For the unpaired trials, rats received a different flavor not followed by the opportunity to run. All rats were then initially tested for water consumption followed by tests of the two flavors (paired or unpaired) in a counterbalanced design. Rats in the UW group show no CTA to the liquid paired with wheel running, whereas LW and HC groups developed CTA. These results indicate that pre-exposure to wheel running (i.e., the UCS), eliminates subsequent CTA.

Some Parameters of Stimulus Preexposure that Affect Conditioning and Generalization of Taste Aversions in Infant Rats

The effects of stimulus preexposure on conditioning and generalization of a taste aversion were evaluated in infant rats, manipulating stimulus similarity and duration, and the length and procedure (intermixed vs. blocked) of preexposure. Preexposure to simple tastes retarded conditioning and reduced generalization (Experiment 1a), whereas preexposure to compound tastes facilitated conditioning and increased generalization (Experiment 1b). Increasing the number of preexposure trials retarded conditioning and decreased generalization with compound tastes (Experiment 1c). These experiments failed to find a differential effect of intermixed vs. blocked stimulus preexposure. In Experiment 2, a 15 min exposure to the conditioned stimulus during conditioning resulted in a weak aversion, whereas a 60-min exposure resulted in a strong aversion. In Experiment 3, the strength of the aversion and the duration of the conditioned stimulus were directly related in nonpreexposed pups, but inversely related in preexposed pups. In infant rats, the level of generalization between stimuli is determined by how preexposure affects acquisition rate.

Latent Inhibition

AbstractLatent inhibition (LI) refers to the deleterious effects of nonreinforced stimulus preexposure on its subsequent conditioning. LI is considered to index an organism's ability to ignore inconsequential stimuli and as such provides a useful tool to measure attentional processes as well as their disruption and enhancement by various pharmacological, physiological and behavioral manipulations. During the last decade, LI has received increasing interest as an animal model of schizophrenia and of antipsychotic drug action. This unit describes the measurement of LI in the conditioned emotional response and two‐way active avoidance procedures, including parametric variations which allow the demonstration of the disruption and the potentiation of this phenomenon.