Constraints from imposed deformations are external constraints that occur, for example, through differential settlements in statically indeterminate systems and are directly proportional to stiffness. The flexural stiffness of reinforced concrete structures is a complex issue that is considerably dependent on concrete cracking. Considering constraints in the design is not easy because many variables must also be considered. The constraint value depends on loading conditions with favourable or unfavourable effects. To obtain realistic results, experimental investigations on constraints derived from differential settlements are conducted. The degree of reinforcement, slenderness, concrete strength, and the effect of constraints are investigated as parameters. The tests showed that the constraint was reduced nearly constantly from the beginning of loading and was no longer present when the maximum load was reached. For this reason, the non-constant stiffness and the effect of stiffness distribution on the internal force distribution could be identified.