Effects Of Resource Competition Research Articles

Effects of resource competition on evolution and adaptive radiation

Effects of resource competition on evolution and adaptive radiation

Leaf senescence, nitrogen remobilization, and productivity of maize in two semiarid intercropping systems

It is unclear how interspecific resource competition affects crop leaf senescence in association with nitrogen (N) remobilization and overyielding in semiarid intercropping systems. To address this issue, maize intercropped with soybean (weakly competitive species) and wheat (strongly competitive species) was examined across three consecutive years to explore the effects of resource competition on maize relative and absolute yield gain, leaf senescence traits, N remobilization and physiological activities in a typical semiarid site. High yield (171.8 g plant−1), relative yield (0.63) and net effect (0.99 t ha−1) of maize were observed in the maizesoybean intercropping system, while the opposite trend was found in the maizewheat intercropping systems. Overall, maize benefited from intercropping with soybean but was negatively affected by intercropping with wheat. Interestingly, both intercropping systems significantly delayed the onset time of maize leaf senescence by approximately 5–20 days and prolonged the senescence duration by approximately 8–18 days. Intercropping with soybean increased maize leaf dry matter remobilization from 7.3 to 12.5 g per plant−1 and reduced N remobilization from 0.81 to 0.45 g plant−1, respectively. However, when intercropped with wheat, dry matter and N remobilization of maize leaves were decreased, at 4.8 and 0.13 g plant−1, respectively. Therefore, excessive delay and prolonged leaf senescence reduced leaf dry matter translocation and N to the grain which impaired yield formation. Our findings provided novel insights into the physiological mechanism of overyielding from the perspective of leaf senescence traits and remobilization function in the semiarid intercropping systems.

Metabolic resource overlap impacts competition among phyllosphere bacteria

The phyllosphere is densely colonised by microbial communities, despite sparse and heterogeneously distributed resources. The limitation of resources is expected to drive bacterial competition resulting in exclusion or coexistence based on fitness differences and resource overlap between individual colonisers. We studied the impact of resource competition by determining the effects of different bacterial colonisers on the growth of the model epiphyte Pantoea eucalypti 299R (Pe299R). Resource overlap was predicted based on genome-scale metabolic modelling. By combining results of metabolic modelling and pairwise competitions in the Arabidopsis thaliana phyllosphere and in vitro, we found that ten resources sufficed to explain fitness of Pe299R. An effect of both resource overlap and phylogenetic relationships was found on competition outcomes in vitro as well as in the phyllosphere. However, effects of resource competition were much weaker in the phyllosphere when compared to in vitro experiments. When investigating growth dynamics and reproductive success at the single-cell resolution, resource overlap and phylogenetic relationships are only weakly correlated with epiphytic Pe299R reproductive success, indicating that the leaf’s spatial heterogeneity mitigates resource competition. Although the correlation is weak, the presence of competitors led to the development of Pe299R subpopulations that experienced different life histories and cell divisions. In some in planta competitions, Pe299R benefitted from the presence of epiphytes despite high resource overlap to the competitor strain suggesting other factors having stronger effects than resource competition. This study provides fundamental insights into how bacterial communities are shaped in heterogeneous environments and a framework to predict competition outcomes.

Interactions between fitness components across the life cycle constrain competitor coexistence.

Numerous mechanisms can promote competitor coexistence. Yet, these mechanisms are often considered in isolation from one another. Consequently, whether multiple mechanisms shaping coexistence combine to promote or constrain species coexistence remains an open question. Here, we aim to understand how multiple mechanisms interact within and between life stages to determine frequency-dependent population growth, which has a key role stabilizing local competitor coexistence. We conducted field experiments in three lakes manipulating relative frequencies of two Enallagma damselfly species to evaluate demographic contributions of three mechanisms affecting different fitness components across the life cycle: the effect of resource competition on individual growth rate, predation shaping mortality rates, and mating harassment determining fecundity. We then used a demographic model that incorporates carry-over effects between life stages to decompose the relative effect of each fitness component generating frequency-dependent population growth. This decomposition showed that fitness components combined to increase population growth rates for one species when rare, but they combined to decrease population growth rates for the other species when rare, leading to predicted exclusion in most lakes. Because interactions between fitness components within and between life stages vary among populations, these results show that local coexistence is population specific. Moreover, we show that multiple mechanisms do not necessarily increase competitor coexistence, as they can also combine to yield exclusion. Identifying coexistence mechanisms in other systems will require greater focus on determining contributions of different fitness components across the life cycle shaping competitor coexistence in a way that captures the potential for population-level variation.

Validation of a new spatially explicit process-based model (HETEROFOR) to simulate structurally and compositionally complex forest stands in eastern North America

Abstract. Process-based forest growth models with spatially explicit representation are relevant tools to investigate innovative silviculture practices and/or climate change effects because they are based on key ecophysiological processes and account for the effects of local competition for resources on tree growth. Such models are rare and are often calibrated for a very limited number of species and rarely for mixed and/or uneven-aged stands, and none are suitable for the temperate forests of Québec. The aim of this study was to calibrate and evaluate HETEROFOR (HETEROgeneous FORest), a process-based and spatially explicit model based on resource sharing, for 23 functionally diverse tree species in forest stands with contrasting species compositions and environmental conditions in southern Quebec. Using data from the forest inventory of Quebec, we evaluated the ability of HETEROFOR to predict the short-term growth (5–16 years) of these species at the tree and stand levels and the long-term dynamics (120 years) of red and sugar maple stands. The comparison between the prediction quality of the calibration and evaluation datasets showed the robustness of the model performance in predicting individual-tree growth. The model reproduced correctly the individual basal area increment (BAI) of the validation dataset, with a mean Pearson's correlation coefficient of 0.56 and a mean bias of 18 %. Our results also highlighted that considering tree position is of importance for predicting individual-tree growth most accurately in complex stands with both vertically and horizontally heterogeneous structures. The model also showed a good ability to reproduce BAI at the stand level, both for monospecific (bias of −3.7 %; Pearson's r=0.55) and multi-species stands (bias of −9.1 %; Pearson's r=0.62). Long-term simulations of red maple and sugar maple showed that HETEROFOR was able to accurately predict the growth (basal area and height) and mortality processes from the seedling stage to the mature stand. Our results suggest that HETEROFOR is a reliable option to simulate forest growth in southern Quebec and to test new forestry practices under future climate scenarios.

Architectural effects regulate resource allocation within the inflorescences with nonlinear blooming patterns.

Spatial and temporal resource allocations within inflorescences have been well-studied in many plants based on flowering sequence or floral position. However, there had been few attempts to investigate architectural effects and resource competition in species where the blooming pattern does not follow a linear positional pattern within the inflorescence. Moreover, most flowering plants show female-biased sex allocation in early or basal flowers, but it is unclear in species with inherent and changeless ovule production. We investigated intra-inflorescence variation in reproductive traits of Salvia przewalskii, a perennial herb with 4-ouvle ovary flowers and flowering sequence-floral position decoupled inflorescences. To detect the effects of resource competition and architectural effects on reproductive success, we manipulated inflorescence (removed floral buds by position and flowering sequence) and pollination (opened and supplemented pollination). Pollen production and dry mass deceased from bottom to top flowers but did not significantly differ following flowering sequence, resulting in male-biased sex allocation in basal flowers. The seed production, fruit set, and bud development exhibited significant declining trends from proximal to distal positions regardless of the thinning and pollen treatments. Meanwhile, the seed production, fruit set, and bud development success did not significant differ when thinning was conducted according to flowering sequence. Architectural effects plays a crucial role in resource allocation within decoupled flowering inflorescences. Moreover, our results highlighted that inherent floral traits such as changeless ovule production, may modify architectural effects on sex allocation.

Heterogeneity in resource competition covaries with individual variation in long-term social relationships

Abstract Resource competition among conspecifics is central to social evolution, as it serves as one of the primary selective pressures of group living. This is because the degree of competition for resources impacts the costs and benefits of social interactions. Despite this, how heterogeneity in resource competition drives variation in the type and quantity of long-term social relationships individuals foster has been overlooked. By measuring male mating competition and female foraging competition in a highly social, long-lived mammal, we demonstrate that individual variation in long-term intrasexual social relationships covaries with preferred habitat and experienced resource competition, and this effect differs based on the sex of the individual. Specifically, greater resource competition resulted in fewer social preferences, but the magnitude of the effect varied by both habitat and sex, whereas for social avoidances, both the directionality and magnitude of the effect of resource competition varied by habitat and sex. Together our work shows how fine-scale variation in individual socioecological niches (i.e., unique physical and social environments) can drive extensive variation in individual social behavior (here long-term relationships) within a population, thereby broadening current theories of social evolution.

Emergent correlations in gene expression dynamics as footprints of resource competition.

Genetic circuits need a cellular environment to operate in, which naturally couples the circuit function with the overall functionality of gene regulatory network. To execute their functions, all gene circuits draw resources in the form of RNA polymerases, ribosomes, and tRNAs. Recent experiments pointed out that the role of resource competition on synthetic circuit outputs could be immense. However, the effect of complexity of the circuit architecture on resource sharing dynamics is yet unexplored. In this paper, we employ mathematical modelling and in-silico experiments to identify the sources of resource trade-off and to quantify its impact on the function of a genetic circuit, keeping our focus on regulation of immediate downstream proteins, which are often used as protein read-outs. We show that estimating gene expression dynamics from readings of downstream protein data might be unreliable when the resource is limited and ribosome affinities are asymmetric. We focus on the impact of mRNA copy number and ribosome binding site (RBS) strength on the nonlinear isocline that emerges with two regimes, prominently separated by a tipping point, and study how correlation and competition dominate each other depending on various circuit parameters. Focusing further on genetic toggle circuit, we have identified major effects of resource competition in this model motif and quantified the observations. The observations are testable in wet-lab experiments, as all the parameters chosen are experimentally relevant.

Invading parasites: spillover of an alien nematode reduces survival in a native species

It is widely assumed that spillover of alien parasites to native host species severely impacts naïve populations, ultimately conferring a competitive advantage to invading hosts that introduced them. Despite such host-switching events occurring in biological invasions, studies demonstrating the impact of alien macroparasites on native animal hosts are surprisingly few. In Europe, native red squirrels (Sciurus vulgaris) are replaced by introduced North American grey squirrels (S. carolinensis) mainly through resource competition, and, only in the United Kingdom and Ireland, by competition mediated by a viral disease. In Italy such disease is absent, but spillover of an introduced North American nematode (Strongyloides robustus) from grey to red squirrels is known to occur. Here, we used long-term (9 years) capture-mark-recapture and parasitological data of red squirrels in areas co-inhabited by grey squirrels in Northern Italy to investigate the impact of this alien helminth on naïve native squirrels’ body mass, local survival, and reproduction of females. We found no negative effect of the alien parasite on body mass or reproductive success, but intensity of infection by S. robustus reduced survival of both male and female squirrels. Significantly, survival of squirrels co-infected by their native nematode, Trypanoxyuris sciuri, was less affected by S. robustus, suggesting a protective effect of the native helminth against the new infection. Hence, we demonstrate that alien S. robustus spillover adds to the detrimental effects of resource competition and stress induced by grey squirrels, further reducing the fitness of the native species in the presence of the invasive competitor.

Size-asymmetric competition among snails disrupts production of human-infectious Schistosoma mansoni cercariae.

Parasites can harm hosts and influence populations, communities, and ecosystems. However, parasites are reciprocally affected by population- and community-level dynamics. Understanding feedbacks between infection dynamics and larger-scale epidemiological and ecological processes could improve predictions and reveal novel control methods. We evaluated how exploitative resource competition among hosts, a fundamental aspect of population biology, influences within-host infection dynamics of the widespread human parasite Schistosoma mansoni in its intermediate host, Biomphalaria glabrata. We added size-dependent consumption of shared resources to a parameterized bioenergetics model to predict a priori the growth, parasite production, and survival of an infected focal host coexisting with an uninfected conspecific competitor in an experiment that varied competitor size. The model quantitatively anticipated that competitors disrupt growth and parasite production and that these effects increase with competitor size. Fitting the model to these data improved its match to host survivorship. Thus, resource competition alters infection dynamics, there are strong size asymmetries in these effects, and size-asymmetric resource competition effects on infection dynamics can be accurately predicted by bioenergetics theory. More broadly, this framework can assess parasite transmission and control in other contexts, such as in resource competitive host communities, or in response to eutrophication, food supplementation, or culling.

Agent-based model for post-earthquake housing recovery

A framework of agent-based models for housing recovery is presented and used to investigate post-earthquake recovery in the City of Vancouver, Canada. Housing recovery is modeled for a portfolio of buildings, contrasting with the practice of assessing the reconstruction of buildings in isolation. Thus, the presented approach better captures the effect of competition for resources, infrastructure disruptions, and socioeconomic factors on recovery. The analyses include models for damage, inspection, financing, power infrastructure, and labor/materials for repairs. The presented approach is applied to simulate the recovery of 114,832 residential buildings in 22 neighborhoods in Vancouver. Results indicate that recovery after a strong earthquake will take more than three years. The density of old and rented buildings, and the income and immigration status of the homeowners are shown to be good predictors of the speed of recovery for a neighborhood. Mitigation measures are compared and it is shown that retrofitting the most physically vulnerable buildings or doubling the available workforce are effective at reducing housing recovery times. It is demonstrated that the equity in recovery between low and high socioeconomic status homeowners is improved if mitigation measures are implemented. The results presented in this article can inform disaster recovery plans and mitigation actions in Vancouver and similar communities.

Effect of Tree Presence and Soil Characteristics on Soybean Yield and Quality in an Innovative Alley-Cropping System

Agroforestry is indicated as a farming practice suited to enhance ecosystem services generated by cropping systems. However, farmers are often reluctant to implement agroforestry systems due to the potential yield loss of crops. In a field trial, soybean was intercropped with poplar short-rotation-coppice rows in an alley-cropping system with 13.5 m wide alleys, in order to assess the effect of tree presence on soybean yield and quality. The light availability (LA) was significantly affected by the tree presence, with an increasing effect along the season due to tree growth, being at its lowest in the tree–crop interface positions (West and East). Significant effects of the tree-distance and LA were registered on soybean yield, with the highest reduction of soybean yield in the West (−78%) and East (−35%) positions. Crude fat content in soybean grain did not vary among positions in the alley, while crude protein content was the lowest in the most shaded position (West −8% than the highest value). The assessment of spatial variability among plots of measured soil characteristics highlighted no significant effect of pedological conditions upon soybean yield. Thus, in our study, the LA effect was more important than soil characteristics in determining a detrimental effect of competition for resources between soybean and poplar. Conversely, soybean quality was affected by soil characteristics, since crude fat significantly correlated with soil nutrients, pH, soil organic matter and soil texture.

Professional Friendship, Resource Competition, and Collaboration in a Homeless Service Delivery Network

ABSTRACTWhile existing literature on human services interorganizational network has focused on collaboration, networked service providers do compete for the same public and private funding sources. Interorganizational competition for scarce resources could be detrimental to collaboration. Yet organizational leaders’ external professional friendship ties with peers – an understudied type of informal relationship – may mitigate the effects of resource competition. Using data collected from a homeless service delivery network, we investigate such relational dynamics. Quantitative network analysis shows that professional friendship ties facilitate collaboration and moderate the negative impact of competition on collaboration. Both funders and managers need to invest in developing professional friendship networks.

Resource competition and allelopathy in two peat mosses: implication for niche differentiation

Separating the effect of resource competition from allelopathy in plants is challenging and it has never been attempted in closely related co-occurring bryophytes. In peatlands, peat mosses (Sphagnum spp.) show niche differentiation along water table level (WTL) gradient. Our aim was to evaluate whether the hummock species, S. magellanicum would be a winner at low WTL due to its allelopathic advantage and the hollow species, S. angustifolium would win by virtue of its superior competitive ability but not of allelopathy at high WTL due to dilution of its allelochemicals. We used a nested, field experimental design, with two WTL treatments—low WTL (hummock habitat) and high WTL (hollow habitat)—and three different inter-specific interactions: 1) monoculture; 2) mixed culture without activated charcoal; and 3) mixed culture with activated charcoal added to the neighbor. We measured growth and biochemical traits of the two species and compared the index of relative neighbor effect on each other. We discovered a trade-off between biomass production (competitive outcome) and phenolic content (allelopathy) in these species. At low WTL, allelopathy of the hummock species is the main mechanism to suppress the hollow species, whereas at high WTL, competition is the main driver to suppress the hummock species. Competitive advantage in Sphagnum is mediated by both resource competition and allelopathy of the co-occurring species through niche differentiation along a WTL gradient. Unlike vascular plants, Sphagnum mosses can serve as excellent model organisms in studying allelopathic interaction since they bypass the complexity of plant-soil interactions.

Combined Effects of Natural Enemies and Competition for Resources on a Forest Defoliator: A Theoretical and Empirical Analysis.

Explanations for the dynamics of insect outbreaks often focus on natural enemies, on the grounds that parasitoid and pathogen attack rates are high during outbreaks. While natural enemy models can successfully reproduce outbreak cycles, experiments have repeatedly demonstrated the importance of resource quality and abundance. Experiments, however, are rarely invoked in modeling studies. Here we combine mechanistic models, observational data, and field experiments to quantify the roles of parasitoid attacks and resource competition on the jack pine budworm, Choristoneura pinus. By fitting models to a combination of observational and experimental data, we show that parasitoid attacks are the main source of larval budworm mortality at low and intermediate budworm densities but that resource competition is the main source of mortality at high densities. Our results further show that the effects of resource competition become more severe with increasing host tree age and that the effects of parasitoids are moderated by strong competition between parasitoids for hosts. Allowing for these effects in a model of insect outbreaks leads to realistic outbreak cycles, while a host-parasitoid model without resource competition produces an unrealistic stable equilibrium. The effects of resource competition are modulated by tree age, which in turn depends on fire regimes. Our model therefore suggests that increases in fire frequency due to climate change may interact in complex ways with budworm outbreaks. Our work shows that resource competition can be as important as natural enemies in modulating insect outbreaks, while demonstrating the usefulness of high-performance computing in experimental field ecology.

Carry-over effects of resource competition and social environment on aggression

AbstractAggressive behavior is common in many species and is often adaptive because it enables individuals to gain access to limited resources. However, aggression is also highly plastic and the degree of plasticity could be influenced by factors such as resource limitation and the social environment. In this study, we examined how the effects of social experience and resource limitation could persist to affect future aggressive interactions. Using naturally inbred strains of Drosophila melanogaster that differ in aggressiveness, we manipulated the level of available resources by varying fly density (two treatments: high and low per capita resources) and group composition by varying strain frequency (five treatments: homogeneous strains, or mixed at 1:3, 1:1 or 3:1 ratios of the more aggressive to less-aggressive strain). For each treatment group, we measured aggression before and after flies were placed through a 4-day period of fixed resources. There was no consistent effect of resource competition on aggression. Instead, changes in aggression depended on resource availability in combination with group composition. In homogeneous groups made up of only one strain, all males became more aggressive following the fixed-resource period, regardless of fly density. In mixed-strain treatments at high density, we observed plastic shifts in aggression of males from both strains, but the direction of plastic responses depended on social composition. Our results show that aggression may not only be influenced by the intensity of previous competitive experiences caused by resource limitation, but also through social effects caused by the composition of the group.

The measurement of interpersonal interactions with continuous spatiotemporal data: Application to a study of the effects of resource competition on racial group interactions.

We describe a sequential qualitative ➔ quantitative mixed-method procedure used to construct conceptually grounded quantitative metrics of interpersonal behavior from continuous spatiotemporal data. Metrics were developed from data collected during an experiment in which racially diverse participants interacted with self-resembling avatars at social events hosted in the virtual world Second Life. In the qualitative stage, the researchers conceptualized four distinct patterns of movement from overhead video recreations of participants interacting during the social events. In the quantitative stage, these patterns of movement were operationalized into metrics to reflect each type of observed interpersonal behavior. The metrics were normalized through a series of transformations, and construct validity was assessed through correlations with self-report measures of intergroup behavior. Finally, the metrics were applied to an analysis of the virtual-world study examining the influence of resource competition on racial group interactions. The findings contribute to our understanding of the influence of resource competition on Blacks', Asians', and Whites' group dynamics. Applications of these metrics for the future of the psychological study of interpersonal behavior are discussed.

Modeling light availability for crop strips planted within apple orchard

Rational design and management of the crop strips structure within fruit orchards are essential to make use of the economic and ecological advantages of intercropping but avoid the negative effects of resource competition on the fruit production. A better understanding of the light transmission processes could provide basis for quantifying resource competition and growth dynamics in the agroforestry system. Modeling work was combined with field experiment in this study to investigate light transmission in an apple tree (Malus pumila M.) and cocksfoot (Dactylis glomerata L.) intercropping system. The experiment was conducted in 2016 and 2017 in a plantation of apple at a spacing of 4 m × 4 m on the Loess Plateau of China. Three floor managing patterns were applied: clean tillage (CT), 2.4-meter-wide cocksfoot strips set up between tree rows that harvested more frequently to maintain a low coverage (LC), and cocksfoot strips with a greater coverage (GC). The light model simulates light transmission in the tree crown with a geometrical model that considering the canopy spatial heterogeneity, and describes light extinction in the crop strips with a strip-path radiation transmission model which is suitable for strip-path canopy structures. Results showed that the model well predicted fraction of incident light over and transmitted through the cocksfoot strips with determination coefficient of 0.707 and 0.892 respectively and root mean square error of 0.096 and 0.037. Averaged over the two seasons, light interception by cocksfoot were 439.1 and 504.7 MJ m−2 in LC and GC respectively, accounted for 23.1% and 26.5% of the total incoming light. Light use efficiency of cocksfoot in LC was far greater than that in the GC in both seasons. Planting cocksfoot showed no significant adverse effects on apple production, 6.32 and 5.67 t ha−1 additional dry forage were produced in the LC and GC plots respectively in 2016, the production were only 3.02 and 2.07 t ha−1 in 2017 as limited by water availability. Simulations with information on apple tree orchards of different age showed that seasonal mean fraction of light interception by cocksfoot under 3–10 years old apple trees varied from 0.41 to 0.29 for GC and from 0.36 to 0.25 for LC treatments, but the fraction became <0.15 for 15 years and older orchards. This study provides basics for quantifying light and other resource competition in tree and crop intercropping systems and gives insights into floor management for rain-fed apple orchards on the Loess Plateau area of China.

Variability in space and time: contrasting fruit distribution patterns in the deceptive orchid Orchis militaris

In angiosperms, a decrease in fruit production towards the apex of individual inflorescences is usually observed. Orchids are thought to be primarily pollination-limited species, and non-uniform pollination could cause this decrease pattern in several species. Fruit production was investigated in relation to flower position and floral display size in Orchis militaris (Orchidaceae), a deceptive species. Over 2years, eight populations of O.militaris were studied and fruit position along the inflorescence was recorded. Generalised linear models were performed to examine the effect of population, year, flower position and floral display size on fruit production. The dominant pattern was characterised by a higher fruit set in the middle part of the inflorescence (parabolic pattern). A non-directional pattern of fruit production was also detected in some populations. Within a given population, patterns were generally consistent among years. In one of the two study years and in one of the eight populations specifically, the proximal-to-distal decrease in fruit production was dramatic in plants with a large floral display but weak or absent in small displays. Our study demonstrates the intraspecific diversity of fruit distribution patterns in O.militaris. Non-uniform pollination along the inflorescence is likely to be responsible for the parabolic pattern, while irregular visitation could explain the non-directional pattern of fruit production. Pattern variation among years and between populations could arise from spatiotemporal variation in pollinator assemblages. Resource competition effects could explain the interaction effect between display size and flower position.

Vertical foraging shifts in Hawaiian forest birds in response to invasive rat removal.

Worldwide, native species increasingly contend with the interacting stressors of habitat fragmentation and invasive species, yet their combined effects have rarely been examined. Direct negative effects of invasive omnivores are well documented, but the indirect effects of resource competition or those caused by predator avoidance are unknown. Here we isolated and examined the independent and interactive effects of invasive omnivorous Black rats (Rattus rattus) and forest fragment size on the interactions between avian predators and their arthropod prey. Our study examines whether invasive omnivores and ecosystem fragment size impact: 1) the vertical distribution of arthropod species composition and abundance, and 2) the vertical profile of foraging behaviors of five native and two non-native bird species found in our study system. We predicted that the reduced edge effects and greater structural complexity and canopy height of larger fragments would limit the total and proportional habitat space frequented by rats and thus limit their impact on both arthropod biomass and birds’ foraging behavior. We experimentally removed invasive omnivorous Black rats across a 100-fold (0.1 to 12 ha) size gradient of forest fragments on Hawai‘i Island, and paired foraging observations of forest passerines with arthropod sampling in the 16 rat-removed and 18 control fragments. Rat removal was associated with shifts in the vertical distribution of arthropod biomass, irrespective of fragment size. Bird foraging behavior mirrored this shift, and the impact of rat removal was greater for birds that primarily eat fruit and insects compared with those that consume nectar. Evidence from this model study system indicates that invasive rats indirectly alter the feeding behavior of native birds, and consequently impact multiple trophic levels. This study suggests that native species can modify their foraging behavior in response to invasive species removal and presumably arrival through behavioral plasticity.