Effect Of Pyridoxine Supplementation Research Articles

The effect of pyridoxine supplementation on quality of life of patients with chronic lymphocytic leukaemia

Objective : Pyridoxine, is essential in the metabolism of many classes of food, we aimed at determining the effect of its supplementation on the quality of life (QoL) of patients with chronic lymphocytic leukaemia (CLL). Methods : This study compared the (QoL) and haematological parameters of CLL patients before and after the administration of pyridoxine. Data obtained were analyzed using SPSS version 19. Results : There was improvement in the QoL of the patients after pyridoxine supplementation; Majority of the parameters that make up the physical functional scales was significantly higher after pyridoxine supplementation. There were also significant improvements in insomnia, appetite loss and constipation after pyridoxine supplementation. Conclusion : Pyridoxine supplementation in patients with CLL marginally improved quality of life. Keywords : Pyridoxine, Supplementation, Quality of life, Leukaemia, CLL

Effects of pyridoxine supplementation or in combination with other antioxidants on motility, in vitro capacitation and acrosome reaction of goat buck spermatozoa during cryopreservation

Effects of semen extenders supplemented with pyridoxine or in combination with other antioxidants on motility, in vitro capacitation and acrosome reaction of spermatozoa obtained from West African Dwarf (WAD) goat bucks during cryopreservation were studied. Tris-based extenders supplemented with 2, 4, 6 and 8mM of pyridoxine in study I, and 2mM of pyridoxine (P) supplementation combined with 2mM of vitamin E, vitamin C and melatonin (PE, PC, PM and PCEM) each in study II, were diluted with pooled semen samples. Sperm motility, capacitation and acrosome reaction were assessed following cryopreservation. The results showed higher (P<0.05) sperm motility and acrosome reaction in extenders supplemented with pyridoxine alone or combined with vitamin C, vitamin E and melatonin compared to the control. The improvement in sperm motility was optimal in extenders supplemented with 6mM and PC while the highest percentage of capacitation was observed in extenders supplemented with 4mM, 6mM, PE and PCEM compared to other treatments and the control (P<0.05). The findings revealed that pyridoxine alone at 6mM was consistently effective for improving motility, capacitation and acrosome reaction of cryopreserved spermatozoa in Tris-egg yolk extender of WAD goat bucks during cryopreservation.

Effects of Dietary Pyridoxine on Growth and Physiological Responses of Labeo rohita Fingerlings Reared in High Water Temperature

The role of dietary pyridoxine (vitamin B6) on the growth and physiological responses of Labeo rohita fingerlings reared in a high temperature of 33°C was examined in a 60-day trial. Two hundred and seventy fingerlings (6.71±0.32 g) were randomly distributed into six treatments in triplicate. Five iso-caloric and iso-nitrogenous purified diets were prepared with graded levels of pyridoxine: 0, 10, 50, 100, and 200 mg. The control group was given a diet containing 10 mg pyridoxine and reared at ambient temperature (26°C). Pyridoxine supplementation at 100 or 200 mg/kg diet significantly augmented the specific growth rate but there were no significant (p&gt;0.05) effects of pyridoxine supplementation on the feed conversion ratio. Superoxide-dismutase, catalase, blood glucose, and serum cortisol were significantly lower and acetylcholinesterase (AchE) significantly (p&lt;0.05) higher in the pyridoxine-fed groups. Results indicate that dietary pyridoxine supplementation at the rate of 100 mg/kg diet augments growth and helps lower stress in L. rohita fingerlings reared in a high water temperature.

Pyridoxine supplementation for the treatment of levetiracetam-induced behavior side effects in children: Preliminary results

Behavioral side effects related to the use of levetiracetam (LEV) in epilepsy are increasingly being recognized. Patients followed in our center have reported improvement of these side effects after starting pyridoxine (vitamin B(6)) supplements. Using mailed questionnaires, retrospective chart reviews, and phone call follow-ups, we analyzed 42 pediatric patients who had been treated with LEV and pyridoxine. Twenty-two patients started pyridoxine after being on LEV, and significant behavioral improvement was observed in nine (41%), no effect in eight (36%), deterioration in four (18%), and an uncertain effect in one. The effects of pyridoxine supplementation were observed during the first week. The remaining patients (20) were already on pyridoxine before LEV was started, started pyridoxine and LEV at the same time, or took pyridoxine intermittently. Pyridoxine is an easily available, inexpensive, and safe therapeutic option. Given these preliminary results, we plan to conduct a placebo-controlled cross-over study to better characterize these observations.

The effect of pyridoxine supplementation on dietary protein utilization in gilthead seabream fry

A study was undertaken to ascertain the effect of pyridoxine in diets of varying protein to energy ratio (P: E) for Mediterranean gilthead seabream fry (Sparus aurata) with respect to protein utilization. Diets were formulated to provide three levels of added pyridoxine (0·5, 5·0 and 50·0 mg/kg), at two protein levels (320 or 470 g/kg) whilst maintaining a similar gross isocalorific profile at 20 MJ/kg. Feeding level was proportionately 0·02 of the fish body weight per day, resulting in the following effects after a 70-day feeding trial. Low P: E ratio diets (15 g/MJ) did not perform as well as high P: E diets (24 g/MJ), with respect to growth related indices. Higher inclusions of pyridoxine into diets of equal P: E ratios, caused elevations in food conversion efficiency, protein efficiency ratio and apparent net protein utilization. A marked improvement of growth rate concomitant with pyridoxine level only occurred in the high P: E diets. It was concluded that incremental additions of pyridoxine in the diet, increased the efficiency of protein utilization for all diets tested. However, the protein sparing effect oflipid was not achieved for the low protein diets indicating the relatively high requirement for protein in the growth of the juvenile seabream.

Effects of Pyridoxine Supplementation on Blood Glucocorticoids Level, Aspartate Aminotransferase Activity and Glucose Tolerance Pattern Under Acute Hypoxic Stress

Studies were conducted on male adult rabbits to find out the changes in blood glucocorticoid levels along with the changes in aspartate aminotransferase activity in blood and the role of pyridoxine on the glucose tolerance pattern under hypoxic stress. Hypoxic stress was produced by exposing the animals to a simulated altitude of 7,000 m for 6 h. In the first set of experiments 10 rabbits were used. Blood haemoglobin level, plasma and erythrocyte glucocorticoid levels and erythrocyte GOT activity were measured just before and after the exposure to hypoxia. Erythrocyte GOT activity was measured both without and with 50 mg of pyridoxal phosphate addition to the incubation mixture. Glucocorticoid levels in plasma increased by 11% whereas in erythrocytes the increase was 55% after hypoxia. Percent stimulation of erythrocyte GOT activity with pyridoxal phosphate before exposure to hypoxia was 180% but increased to 321% after exposure. In the second set of experiments another 10 rabbits were used. First they were exposed to hypoxia without pyridoxine hydrochloride feeding and then after 7 days with 3 mg of pyridoxine hydrochloride feeding. For glucose tolerance tests the animals were fed with 1 g of glucose immediately after the hypoxic exposures. Plasma reduced glutathione (GSH), LDH and ICDH activities increased and GOT activity was depressed after hypoxic stress, but when the animals were fed pyridoxine hydrochloride prior to the exposure the enzyme activities remained unaltered after hypoxic stress. Pyridoxine hydrochloride did not alter the pattern of glucose tolerance after hypoxic stress.

Effect of pyridoxine supplementation on plasma oxalate concentrations in patients receiving dialysis

Plasma oxalate and erythrocyte glutamic oxaloacetate transaminase activity (EGOT) (an indicator of nutritional status with respect to pyridoxine) were measured in 21 patients maintained on regular continuous ambulatory peritoneal dialysis or haemodialysis before and after a 4-month period of supplementation with pyridoxine, 100 mg day-1. Prior to supplementation 10/21 patients showed subnormal EGOT activity, although the increment in activity on addition of pyridoxal-5-phosphate in vitro was within the normal range in all cases. Mean plasma oxalate was 31.5 mumol l-1 (SEM 2.9) prior to supplementation and did not change significantly with supplementation, despite normalization of EGOT activity in all but 2/21 patients. We conclude that pyridoxine deficiency does not contribute significantly to hyperoxalaemia in patients receiving dialysis and that 100 mg of pyridoxine daily is insufficient to reduce oxalate generation by a pharmacological action on glycine transamination.

Pyridoxine supplementation: effect on lymphocyte responses in elderly persons

The effect of pyridoxine supplementation on lymphocyte responsiveness was investigated in 15 persons aged 65–81 y. Eleven subjects received 50 mg/d pyridoxine HCl (PN). Four subjects received a placebo. Lymphocyte proliferation to T and B cell mitogens, lymphocyte subpopulations with monoclonal antibodies, and plasma pyridoxal 5′-phosphate (PLP) were measured before and after 1 and 2 mo of supplementation. After 1 and 2 mo plasma PLP levels increased by 195 +/- 88 nM and 201 +/- 84 nM, respectively, in subjects receiving PN. With PN supplementation, lymphocyte proliferation increased significantly in response to phytohemagglutinin (p less than 0.01), pokeweed mitogen (p less than 0.01), and Staphylococcus aureus (Cowain I) (p less than 0.05). For PN-treated subjects with low presupplement plasma PLP levels, lymphocyte blastogenesis also increased significantly (p less than 0.01) in response to concanavalin A. Percentages of T3+ and T4+ but not T8+ cells increased significantly (p less than 0.05) in PN-treated subjects. These results suggest that improving vitamin B-6 status is important in stimulating immunocompetence in the elderly.

Homocystinuria: the effect of pyridoxine supplementation on cultured skin fibroblasts.

Six skin fibroblast cultures, two derived from pyridoxine responsive homocystinurics, two from pyridoxine non-responsive homocystinurics, one from an obligate heterozygote and one from a normal control were grown for three passages in the presence of 9.7 mu mol/l, 243 mu mol/l and 486 mu mol/l of pyridoxine respectively. Cystathionine beta-synthase activity was stimulated in all four homocystinuric cell strains at 486 mu mol/l; aspartate and alanine aminotransferase activity was also increased, particularly in the pyridoxine responsive cell strains. There was, however, evidence of cytotoxicity at the pyridoxine levels of 243 mu mol/l and 486 mu mol/l.

Effect of Pyridoxine Supplementation on Glutamic-Pyruvic Transaminase and In Vitro Stimulation in Erythrocytes of Normal Women

Effect of Pyridoxine Supplementation on Glutamic-Pyruvic Transaminase and In Vitro Stimulation in Erythrocytes of Normal Women