Effects Of Pinaverium Bromide Research Articles

Improvement of Quality of Nonanesthetic Colonoscopy by Preoperative Administration of Pinaverium Bromide.

Background:Nonanesthetic colonoscopy is popular in clinical practice in China. However, intestinal spasms often result in a prolonged examination time, increased operating difficulties, decreased polyp detection rate, and failure to complete the procedure clinically. Therefore, exploring alternative approaches that can reduce the pain in patients during colonoscopy is of utmost importance, and finding the optimal preoperative administration to improve the quality of nonanesthetic colonoscopy is also necessary. This study aimed to investigate the effects of the prophylactic administration of pinaverium bromide before colonoscopy and the effects of pinaverium bromide alone at different time points or combined with scopolamine butylbromide.Methods:A randomized controlled trial was performed on a cohort of 1000 patients who underwent colonoscopy in outpatient clinic of Wuhan Union Hospital. The patients were randomly assigned to the following groups: Group A, given oral pinaverium bromide (100 mg, three times a day) one day before examination combined with intramuscular injection of scopolamine butylbromide (20 mg) 10 min before colonoscopy; Group B0, given pinaverium bromide alone on the day of colonoscopy (100 mg, three times a day); Group B1, given pinaverium bromide alone (100 mg, three times a day) one day before colonoscopy; Group B2, given pinaverium bromide alone (100 mg, three times a day) two days before colonoscopy; and Group C, given scopolamine butylbromide alone (20 mg) before colonoscopy. The successful rate of colonoscopy, procedure time, degree of abdominal pain, and polyp detection rate were recorded and compared among all groups.Results:The successful rate of colonoscopy in Group B1 (82.0%) and Group B2 (83.0%) was significantly higher than that in Group B0 (62.0%, all P < 0.01). The time to reach the ileocecal region in Group B1 and Group B2 were lower than those in Group B0 (all P < 0.05). However, no significant differences were observed in polyp detection rate between Group B1 (24.0%) or Group B2 (26.0%), and Group B0 (22.4%, all P > 0.05). Furthermore, there were no significant differences in the various parameters examined between Group B1 and Group B2 (P > 0.05). The successful rate of colonoscopy in Group A (92.0%) was significantly higher than that in Group B1 (82.0%) and Group C (80.0%; both P < 0.05). Moreover, the time for the colonoscope to reach the ileocecal region in Group A were markedly shorter as compared to those in Group B1 and Group C (P < 0.05). The polyp detection rate in Group A was 32.0%, significantly higher than that in Group B1 (24.0%, P < 0.05) and Group C (24.2%, P < 0.05).Conclusion:Administration of pinaverium bromide alone one day before examination was beneficial to relieve symptoms of abdominal pain during nonanesthetic colonoscopy. In addition, therapeutic effects were improved when pinaverium bromide administration was combined with intramuscular injection of scopolamine butylbromide. Therefore, the combined use of pinaverium bromide with scopolamine butylbromide might have great application value to improve the quality of nonanesthetic colonoscopy in the preoperative preparation.

Observation of effects of pinaverium bromide combined with Paroxetine? Hydrochloride Tablets in treatmentment of irritable bowel syndrome with despondent

Objective To study the therapeutic effects of pinaverium bromide combined with Paroxetine? Hydrochloride Tablets in treatmentment of irritable bowel syndrome(IBS)with despondent. Methods 120patients of IBS with despondent were divided into two groups, One group treated with pinaverium bromide 50 mg tid named Comparison group;the other group treated with pinaverium bromide 50 mg tid combined with Paroxetine? Hydrochloride Tablets 1 sheet per day named treatment group. Results After 2 weeks、4 weeks of treatment, the semiology scores were all decreased in two groups. But in treatment group,the therapeutic effects of all symptom and SDS grading Were significantly higher than Comparison group( P ＜ 0.05 ). Conclusion Pinaverium bromide combined with Paroxetine Hydrochloride Tablets can effectively allevite symptoms of IBS with despondent. Key words: Pinaverium bromide; Paroxetine Hydrochloride Tablets; Despondent; Irritable bowel syndrome

Effects of pinaverium bromide on the development of visceral hypersensitivity and expression of proteinase activated-receptor 2

Objective To investigate the effect of pinaverium bromide on the development of visceral hypersensitivity and the expression of proteinase-activated-receptor 2(PAR2)in rats with water avoidance stress (WAS).Methods The Wistar rats were randomly divided into 3 groups with 12 each.The control group was submitted to sham-WAS and the group A1 was submitted to WAS.The rats in group A2 were submitted to WAS and received pinaverium bromide treatment (30 mg·kg~(-1)·d~(-1)).The stool pellets were counted during WAS and sham-WAS.The magnitude of visceromotor response (VMR) during colorectal distention was used to measure the changes of visceral hypersensitivity.The PAR2 expression was detected by immunofluorescence staining and Western blotting.Results Increased stool pellets were found in group A1 compared to the control group (8.10±2.75 vs.4.13±1.36,P<0.001).Pinaverium bromide treatment decreased stool pellets during WAS (5.87±1.78,P<0.001).The magnitude of VMR (60 mmHg) was higher in group Al compared to the control group (8.64±2.88 vs.3.80±1.04,P<0.001).Pinaverium bromidetreatment could decrease the magnitude of VMR effectively after WAS (6.09±0.77,P=0.02).The expression of PAR2 in group Al was significantly higher than that in the control group (rAU:0.70±0.06 vs.0.32±0.04;M:279.08±100.91 vs.77.16±10.74,P<0.001).The increased PAR2 expression induced by WAS could be inhibited by pinaverium bromide (rAU:0.53±0.06,IA:180.59±25.75,P<0.05).Conclusions Pinaverium bromide may decrease the expression of PAR2 and inhibit the development of visceral hypersensitivity in WAS rats. Key words: Visceral hypersensitivity; Pinaverium bromide; Proteinase activated-receptor 2

Involvement of a CCK-dependent capsaicin-sensitive afferent pathway in the inhibitory effect of pinaverium bromide on the colonic motor response to eating in rats.

The effects of pinaverium bromide on the stimulation of colonic motility induced by meal and cholecystokinin (CCK) were investigated in rats chronically fitted with intraparietal electrodes on the proximal colon and previously treated or not by capsaicin. Pinaverium bromide inhibited in a dose-related manner (2-50 mg/kg, per os) the increase in colonic spike burst frequency induced by a 3 g meal or CCK-8 (2 micrograms/kg, i.v.). The CCK-A and CCK-B antagonists, devazepide and L 365260 (100 micrograms/kg, i.p.), respectively, inhibited the postprandial colonic motor response while only L 365260 reduced the CCK-induced stimulation. The effects of pinaverium bromide and CCK antagonists were not observed in capsaicin-treated animals. Moreover, CCK-8 (2 micrograms/kg, i.v.) did not stimulate colonic motility after capsaicin treatment. The inhibition of postprandial colonic motility by pinaverium bromide, given orally at therapeutic doses, involves a CCK-dependent pathway which requires the integrity of capsaicin-sensitive afferents.

Effect of pinaverium bromide on jejunal motility and colonic transit time in healthy humans

Pinaverium bromide is a specific calcium channel blocker used in the treatment of irritable bowel syndrome (IBS) for its spasmolytic activity. The aim of the present study was to evaluate the effect of orally administered pinaverium bromide on jejunal motility and total and segmental colonic transit time in control subjects. Gastrointestinal studies were performed in 10 healthy volunteers (30 ± 3 years), before and after a treatment phase of 14 days (150 mg/d). Jejunal motility was measured by prolonged manometry (14h) and colonic transit time by a multiple ingestion, single marker technique. No significant modification of phase III of the migrating motor complexes was demonstrated. On the contrary, a significant ( p < 0.01) but weak decrease of the frequency of contraction was found. Unlike previous studies, no decrease of total or segmental colonic transit time was demonstrated. Jejunum, colon / motility / calcium-channel antagonist.

Effects of pinaverium bromide and verapamil on the motility of the rat isolated colon.

Pinaverium bromide was 30 times less potent than verapamil in inhibiting intraluminal pressure responses of in vitro rat colonic segments to barium chloride, acetylcholine, FK 33-824 or field stimulation. The inhibitory effects of both verapamil and pinaverium bromide on the pressure responses to field stimulation were antagonized similarly by exogenous calcium administration. These results support the concept that pinaverium bromide acts on calcium channels in the smooth muscle cell membrane.

Comparison of pinaverium bromide, manganese chloride and D600 effects on electrical and mechanical activities in rat uterine smooth muscle

The effects of pinaverium bromide. were compared with those of D600 and manganese chloride (Mn), on membrane potentials, ionic currents and isometric contractions in uterine smooth muscle strips from pregnant rats. Pinaverium bromide (10 −7-10 −6 M) depressed twitch contractions and K-contractures within 15–20 min while D600 (2 × 10 −6 M) and Mn (10 −3 M) abolished both contractions. D600 and pinaverium bromide were more potent inhibitors in K-depolarized preparations than in polarized tissues. At a supramaximal dose (10 −5 M), pinaverium bromide decreased the rate of rise, amplitude, and rate of repolarization of the action potential, and prolonged the potential duration. The inward Ca current was depressed and the reduction in Ca i was responsible for the decrease in K current. Pinaverium bromide (10 −5 M) depressed the myometrial contractions induced in Ca-free solution by acetylcholine (10 −4 M) and by prolonged membrane depolarizations. Mn (2.5 × 10 −3 M) only reduced the Ach-induced contraction and D600 (10 −5 M) had no effect on intracellular Ca stores. The results indicate that pinaverium bromide has Ca channel blocking properties similar to those of currently used Ca antagonists; it may also exert an effect to depress contractions supported by intracellular Ca release.

Effect of pinaverium bromide on electrical and mechanical activity of smooth muscle cells.

Pinaverium bromide exerts antagonistic effects on the contractions induced by BaCl2 in intestinal smooth muscle. We have therefore investigated its effects on the electrical and mechanical activity and on the 45Ca exchange of guinea-pig taenia coli and ileum. In the concentration range 10−7 to 10−5 M this drug does not exert an effect on the resting potential of either preparation. At 10−6 M no effects could be observed on the spontaneous electrical activity in taenia coli. However, in the ileum the frequency of the action potentials, as well as their amplitude and maximum rate of rise and of repolarization were significantly reduced, causing inhibitory effects on the concomitant mechanical responses. This effect is more pronounced at 10−5 M, and in some tissues the spontaneous activity is completely abolished. Similar effects have been observed in taenia coli at this concentration of pinaverium bromide. The contractions induced by K-depolarization and by 10−5 M carbachol are inhibited in both preparations in a dose-dependent way. Also the contraction induced by carbachol in Ca free solution, as well as the restoration of that contraction by exposing the tissues to Ca containing solutions are reduced in the presence of 10−5 M pinaverium bromide. Pinaverium also inhibits the stimulation of the 45Ca efflux induced by K-depolarization and by stimulation with 10−5 M carbachol. In rabbit ear artery it was found that the contraction induced by noradrenaline is much less sensitive to pinaverium bromide than that induced by depolarization. It was also observed that this substance does not significantly affect the noradrenaline induced contraction in Ca free solution, neither does it prevent the restoration of that contraction by exposure of the tissue to Ca containing solutions. These effects are similar to those observed with other Caentry blocking agents, and therefore suggest that pinaverium bromide mainly acts by blocking voltage-dependent Ca-channels.